9 results about "Environmental toxicology" patented technology

The invention belongs to the field of environmental toxicology and immunology, and particularly relates to an immunotoxicity evaluation method and application thereof. The method comprises the following steps: adding a target pollutant into a culture medium to obtain an exposure culture solution containing the target pollutant, incubating macrophages through the exposure culture solution containing the target pollutant, detecting the macrophages after incubation to obtain results of evaluation indexes, and performing immunotoxicity evaluation based on the results of the evaluation indexes, the target pollutants are chemicals or actual water samples; the evaluation indexes comprise basic cell toxicity, macrophage phagocytic ability and macrophage anti-infection ability. The invention provides a sensitive and reliable immunotoxicity evaluation system, and fills the blank of immunotoxicity evaluation of chemicals, especially raw water. The defects of a traditional concentration method are avoided through a direct exposure method, and the immunotoxicity of the water sample is reflected more truly. The established QSAR model provides a theoretical tool for predicting the immunotoxicity of novel chemicals.

The invention provides an antibiotic mixture toxicity prediction method and device, and belongs to the technical field of environmental toxicology and ecological risk assessment. According to the method, a coupled mixed descriptor of a target antibiotic mixture is obtained through analysis of a minimum redundancy maximum correlation algorithm and a genetic algorithm, and the coupled mixed descriptor sets a corresponding mixing rule for each common descriptor in a plurality of common descriptors in the target antibiotic mixture. The coupled mixed descriptor is analyzed by adopting a neural network prediction model based on a Transform framework, the toxicity prediction value of the target antibiotic mixture is obtained, accurate prediction of the toxicity of the antibiotic mixture can be achieved in the process, environment restoration can be conducted according to the prediction result of the toxicity of the antibiotic mixture, and then environment protection is achieved.

The application discloses a kind of microplastic biological effect evaluation methods based on typical soil animal multilevel response index, it is related to environmental toxicology evaluation and ecological technical field, by setting microplastic treatment group and control group, the multilevel response index of soil animal individual, organ, tissue, molecule level is determined, and the integrated biomarker response index method is used to calculate comprehensive effect index, and constructs microplastic effect comprehensive evaluation model.The application can realize the comprehensive evaluation of microplastic biological effect and early warning of low concentration pollution, improves the comprehensiveness and accuracy of ecological risk assessment, provides reliable technical support for soil microplastic pollution control.

The invention belongs to the technical field of environmental toxicology and biological detection, and particularly relates to a pollutant immunotoxicity comprehensive evaluation method based on a zebra fish model. The invention aims to establish a multi-level and multi-index fused immunotoxicity evaluation system, and by combining indexes such as immune cell quantity, humoral immune factors and immune-related gene expression and constructing a comprehensive immunotoxicity index (ITI), quantitative and comprehensive judgment of pollutant immune injury is realized. Therefore, a scientific basis is provided for rapid identification and risk evaluation of immunotoxicity of environmental pollutants.

This invention belongs to the field of environmental toxicology and molecular detection technology, and provides a rapid and accurate method for detecting ferroptosis induced by environmental pollutants and its applications. This method utilizes early zebrafish embryos as a model organism and combines morphological, biochemical, and molecular biological indicators to establish a standardized ferroptosis detection system for identifying and quantitatively evaluating pesticide exposure-induced ferroptosis. This method features a short detection cycle (completed within 96 hours), high sensitivity, and strong specificity, effectively distinguishing between general oxidative stress and iron-dependent cell death, providing a novel detection tool for pesticide environmental toxicology research and ecological risk assessment.

The invention relates to the technical field of cell culture, in particular to an environment toxicology research cell culture device which comprises a culture box and culture dishes, and a plurality of tray assemblies used for placing the culture dishes are arranged in the culture box; a plurality of indirect boxes in one-to-one correspondence with the tray assemblies are installed at the top end of the incubator, indirect channels communicated with the interior of the incubator are formed in the inner bottom walls of the indirect boxes, and partition assemblies used for controlling the communication condition of the interiors of the indirect boxes and the interior of the incubator are installed in the indirect channels; a plurality of lifting assemblies are mounted on the inner bottom wall of the culture box; the lifting assemblies are used for lifting the culture dishes on the corresponding tray assemblies into the corresponding indirect boxes along the corresponding indirect channels. Through cooperation of the indirect box, the separation assembly and the lifting assembly, independent taking and placing of the culture dishes are achieved; during taking and placing, the indirect channel is closed, so that the incubator can be blocked from being communicated with the outside, and the conditions of temperature, humidity, CO2 concentration and other environment mutation caused by overall box opening are reduced.

The invention relates to the field of environmental toxicology and developmental biology, in particular to a reproductive development toxicity evaluation method based on plastic greenhouse pesticide combined contamination. The combined action toxicity of beta-cypermethrin and emamectin benzoate is systematically evaluated from multiple dimensions of hormone level, ovarian tissue structure, placenta function, progeny development, cell viability and mitochondrial state through the design of combining an in-vivo model and in-vitro cell verification, and the potential hazard mechanism of beta-cypermethrin and emamectin benzoate in the reproductive development process is disclosed. Therefore, a scientific basis is provided for pesticide toxicology risk assessment and safety limit value formulation.

This invention relates to the fields of environmental toxicology and organoid technology, and particularly to a method for assessing the toxicity of environmental microplastic exposure based on testicular organoids. The method includes: processing waste testicular tissue into a single-cell suspension, the single-cell suspension comprising supporting cells, germ cells, and interstitial cells; seeding the single-cell suspension onto a biomimetic hydrogel scaffold for culture to obtain mature testicular organoids, which are then cultured in a target microplastic exposure solution to establish a microplastic exposure model; determining the raw values ​​of toxicity assessment indicators corresponding to the microplastic exposure model, including: morphological score data, grayscale values ​​of cell marker expression, DNA damage indicators, testosterone secretion levels, and cytotoxicity indicators; calculating a comprehensive toxicity score based on the raw values ​​of each toxicity assessment indicator, and assessing the reproductive toxicity risk level of the target microplastic exposure based on the comprehensive toxicity score. This application can improve the accuracy of assessing the reproductive toxicity risk level of microplastic exposure.

The invention discloses a method for detecting early neurodevelopment toxicity of pollutants based on a neural crest specific molecular marker and application, and belongs to the technical field of environmental toxicology and molecular diagnosis. The method comprises the following steps: 1) exposing an early embryo to a to-be-detected sample or pollutant; 2) detecting the change of the specific molecular marker of the neural crest and the peripheral neuron; and 3) evaluating the early neurodevelopmental toxicity according to the marker expression difference. According to the method, the problems of early warning lag and low sensitivity of the traditional neural behavior endpoint (zebra fish 6dpf) are solved, the early neurotoxicity of low-concentration pollutants can be detected within 24 hours of pollutant exposure (zebra fish sox10 and prph), and the method can be used for early toxicity early warning and risk assessment in environmental monitoring and chemical risk assessment.