29 results about "Environmental toxicology" patented technology

The invention relates to the technical field of environmental toxicology and ecological risk assessment, in particular to a system and a method for predicting chronic toxicity of bioaccumulation pollutants, and the system comprises a data acquisition and processing module, a model training and optimization module, a toxicity prediction module and an ecological risk assessment module. According to the method, full-process prediction is realized through data standardization, feature engineering, model training and model prediction; tissue residual toxicity data is obtained and standardized, a multi-dimensional characteristic matrix containing pollutant physicochemical properties, species biological characteristics and experimental conditions is constructed, a toxicity prediction model is optimized by using a machine learning algorithm, and tissue residual toxicity prediction of fishes in different life stages is realized. And calculating a risk quotient based on the predicted value, and generating a risk assessment report. The problems that an existing system depends on water body concentration toxicity data, is lack of biological accumulation pollutant chronic toxicity prediction capacity and does not aim at an endangered characteristic optimization model are solved.

The invention relates to the technical field of environmental toxicology, in particular to a construction method and application of micro-nano plastic combined toxicity (AOP) based on multi-omics integration, and the construction method comprises the following steps: respectively carrying out single exposure culture of multi-target risk substances by adopting human-derived hepatocyte HepaRG cells; determining a combined exposure dose; cell treatment and sample preparation; transcriptome analysis; analyzing metabolome; transcriptome and metabolome data are integrated, pathway cross analysis is carried out, a gene-metabolite interaction network is constructed, and a core regulation and control module is identified; and constructing an AOP framework: determining a molecular initial event, a key event and a harmful outcome of the AOP framework based on the integrated data, and importing an AOP-WIKI platform to generate a standardized AOP framework. According to the method, the limitation of a traditional single omics and fixed dose model is broken through, full-chain dynamic association from a molecular initial event to a harmful outcome is realized, and a scientific basis is provided for combined pollution risk assessment in the fields of environment and food.

The invention provides a method for sequencing new organic silicon pollutants in a surface water body according to toxicology priority indexes, and belongs to the technical field of environmental toxicology and ecological risk assessment. The new organic silicon pollutants in the surface water body are sequenced through comprehensive concentration, detection rate, degradation half-life period, toxicity parameters and multi-dimensional indexes such as binding energy and migration ability obtained through molecular docking analysis; according to the method, a molecular docking technology is introduced into an ecological risk evaluation system for the first time, and key risk factors are highlighted by quantifying binding energy of a target substance and a biological receptor and combining differentiated weight distribution. The method has the advantages that 1) the limitation of a single toxicity index is broken through, and multi-factor comprehensive risk quantification is realized; 2) optimizing environment monitoring and governance resource allocation based on the scientific sorting of ToxPi; and 3) scientificity and accuracy of evaluation are improved, and efficient decision support is provided for prevention and control of organosilicon pollution of surface water.

The utility model relates to the technical field of cell culture, and discloses an environmental toxicology research cell culture device which comprises a culture box, the culture box is internally divided into a culture chamber and an equipment chamber, electric heating pipes are arranged in interlayers of the inner walls of the culture chamber, and the bottom of the culture chamber is communicated with a treatment box through an exhaust pipe I; an exhaust pipe II is arranged at the bottom of the treatment box, a plurality of activated carbon plates are inserted into the treatment box, a plurality of L-shaped brackets are symmetrically arranged in the culture chamber, a placing plate is arranged on the brackets in a sliding manner, a fixing assembly for fixing the placing plate is arranged on the brackets, the fixing assembly comprises a fixing plate, a cavity is formed in each bracket, and the fixing plate is arranged in the cavity. One end of the fixing plate is connected into the cavity through a telescopic spring, a limiting groove is formed in the support, the fixing plate is slidably located in the limiting groove, harmful gas is adsorbed and filtered through the activated carbon plate, pollution to the environment is reduced, the stability of the placing plate is guaranteed through the fixing assembly, and a culture sample is prevented from being scattered and leaked.

The invention belongs to the technical field of biomedicine and environmental toxicology evaluation, and particularly relates to a method for evaluating the risk of pulmonary fibrosis toxicity caused by inhalable micro-nano plastic. The method specifically comprises the following steps: collecting a lung sample into which micro-nano plastic is sucked, and analyzing the lung sample; building a pulmonary fibrosis model; analyzing and calculating the toxicity risk. The invention provides an economical and effective method which is simple and convenient to operate for environmental health monitoring and biomedical research so as to evaluate the potential risk of the micro-nano plastic on the health of the respiratory system of the mammal.

The invention relates to the technical field of cell culture, in particular to an efficient separation culture method for hepatocytes of small yellow croakers and a hepatocyte source identification method. The invention provides a method for efficiently separating and culturing hepatocytes of small yellow croakers, which comprises the following steps: selecting liver tissues of juvenile small yellow croakers, cleaning with a PBS (Phosphate Buffer Solution) containing high-concentration double antibodies, and digesting with pancreatin to quickly obtain hepatocytes; optimizing cell culture to obtain an L-15 culture medium containing 10% of FBS, 1% of sodium pyruvate and 0.2% of NaCl; and cell source identification is carried out by amplifying mitochondrial COI gene and 16S rRNA gene sequences. According to the method, the separation efficiency of the hepatocytes of the small yellow croakers is remarkably improved, the pollution rate is reduced, the result reliability is improved through double-gene molecular identification, the technical blank of construction of hepatocyte lines of the small yellow croakers is filled, and the method has wide application potential in the fields of aquaculture, fish physiology research, environmental toxicology detection and the like.

The invention belongs to the field of environmental toxicology and immunology, and particularly relates to an immunotoxicity evaluation method and application thereof. The method comprises the following steps: adding a target pollutant into a culture medium to obtain an exposure culture solution containing the target pollutant, incubating macrophages through the exposure culture solution containing the target pollutant, detecting the macrophages after incubation to obtain results of evaluation indexes, and performing immunotoxicity evaluation based on the results of the evaluation indexes, the target pollutants are chemicals or actual water samples; the evaluation indexes comprise basic cell toxicity, macrophage phagocytic ability and macrophage anti-infection ability. The invention provides a sensitive and reliable immunotoxicity evaluation system, and fills the blank of immunotoxicity evaluation of chemicals, especially raw water. The defects of a traditional concentration method are avoided through a direct exposure method, and the immunotoxicity of the water sample is reflected more truly. The established QSAR model provides a theoretical tool for predicting the immunotoxicity of novel chemicals.

The invention provides an antibiotic mixture toxicity prediction method and device, and belongs to the technical field of environmental toxicology and ecological risk assessment. According to the method, a coupled mixed descriptor of a target antibiotic mixture is obtained through analysis of a minimum redundancy maximum correlation algorithm and a genetic algorithm, and the coupled mixed descriptor sets a corresponding mixing rule for each common descriptor in a plurality of common descriptors in the target antibiotic mixture. The coupled mixed descriptor is analyzed by adopting a neural network prediction model based on a Transform framework, the toxicity prediction value of the target antibiotic mixture is obtained, accurate prediction of the toxicity of the antibiotic mixture can be achieved in the process, environment restoration can be conducted according to the prediction result of the toxicity of the antibiotic mixture, and then environment protection is achieved.

The invention provides a reclaimed water environment toxicology detection method adopting biomarkers, which comprises the following steps: respectively preparing four biological sensing units by using expanded nephroplasmosis, chlorella vulgaris, zebra fish and jonquil; the four biological sensing units comprise an ecological sensing unit, a photosynthetic sensing unit, a developmental toxicity sensing unit and a plant sensing unit; the method comprises the following steps: continuously pumping a water sample to be detected, filtering suspended impurities, introducing the water sample to be detected into four micro-chambers for culturing, detecting toxicological data after the culture is completed, and respectively placing one of four biological sensing units in each of the four micro-chambers; and performing data processing on the toxicology data, and outputting a toxicity monitoring report. Therefore, the scientific and reliable reclaimed water environment toxicology detection early warning method is constructed, the detection precision and the ecological coverage range are effectively balanced, the limitation of existing chemical indexes is made up, and safe recycling of water resources is guaranteed.

The invention relates to the technical field of environmental toxicology and health risk assessment, in particular to a multi-organ molecular response-based algal toxin health risk prediction and early warning system, which comprises a collection and preprocessing module, a data processing module and an early warning module, the molecular response model construction module is used for constructing a molecular response model; the risk prediction module is used for constructing a risk prediction model; the intelligent early warning and response strategy module is used for matching early warning levels and carrying out color marking and character prompting on the display platform; the graphical display module is also used for marking the geographic position of the sampling section corresponding to the water sample data as a high-risk area and performing graphical display; and the management module is also used for outputting management suggestions for assisting decision response according to the health indexes and the early warning levels in a matching manner and pushing the management suggestions to the supervision terminal or the APP for reminding. The technical bottlenecks of an existing algal toxin health risk assessment and early warning system in the aspects of toxicity mechanism deficiency, model non-migration, early warning response lag and the like are overcome.

The application discloses a method for evaluating environmental risks of drugs in a water environment based on a fish plasma model, and belongs to the technical field of environmental toxicology and ecological risk assessment. The fish plasma-water distribution coefficient is calculated by a drug lipophilicity parameter (Log Dow), the fish steady-state plasma concentration is obtained by combining the measured / predicted environmental concentration, the fish treatment plasma concentration is converted by the difference between the half-inhibitory concentrations of the drug on fish and human, the pharmacological effect risk of the drug on fish is quantitatively evaluated by a risk quotient, and thus high-risk drugs are screened. Compared with the traditional risk quotient method, the application has the advantages of being in line with the drug action mechanism, taking into account the in-vivo enrichment effect, being high in evaluation accuracy, and not needing to rely on a large amount of acute / chronic toxicity data, and can efficiently support the priority control and risk management of drug pollutants in the water environment.

The invention discloses a subtropical estuary benthic fish mullet chubil AHR1b gene recombinant expression plasmid and application thereof, and belongs to the technical field of environmental toxicology. The AHR1b gene recombinant expression plasmid is prepared by introducing an AHR1b gene into an expression vector. The invention also discloses a luciferase reporter gene system, which comprises an AHR1b gene recombinant expression plasmid, an ARNT1 gene recombinant expression plasmid and a firefly luciferase reporter plasmid containing a mouse CYP1A1 gene promoter. According to the invention, a luciferase reporter gene system is utilized to carry out comprehensive characterization on TCDD toxicity and evaluate the sensitivity and the specificity of a mugilogobius chuchuyi AHR1b and ARNT1 subtype dependency reporter gene system, so that a theoretical basis is provided for supporting high-throughput and high-sensitivity environment monitoring, and meanwhile, a species-specific early warning technology is provided for offshore and estuary DLCs pollution.

The application discloses a kind of microplastic biological effect evaluation methods based on typical soil animal multilevel response index, it is related to environmental toxicology evaluation and ecological technical field, by setting microplastic treatment group and control group, the multilevel response index of soil animal individual, organ, tissue, molecule level is determined, and the integrated biomarker response index method is used to calculate comprehensive effect index, and constructs microplastic effect comprehensive evaluation model.The application can realize the comprehensive evaluation of microplastic biological effect and early warning of low concentration pollution, improves the comprehensiveness and accuracy of ecological risk assessment, provides reliable technical support for soil microplastic pollution control.

The invention relates to the technical field of biomedical detection and environmental toxicology evaluation, and discloses a method for detecting caenorhabditis elegans closed-loop self-adaptive neurological function based on a specific neural circuit probe, which comprises the following steps: S1, preparing a specific neural circuit probe caenorhabditis elegans strain, S2, exposing the caenorhabditis elegans strain to a pollutant to be detected, and S3, detecting the caenorhabditis elegans closed-loop self-adaptive neurological function. The method comprises the steps of S1, carrying out closed-loop adaptive neural function detection on a nematode strain, S4, generating a control input signal based on errors through a digital controller, S5, synchronously collecting time sequence data of actual behavior output and the control input signal, S6, quantifying a neural adaptive damage characteristic spectrum index, and S7, completing neurotoxicity evaluation based on the index. According to the method, high-sensitivity detection on neurotoxicity is realized, a nervous system executes a continuous tracking task, when the function of a neural circuit is only slightly reduced, the control effort paid by the system for maintaining the task is remarkably increased, and sub-lethal nerve injury which is difficult to find by a traditional method can be quantified.

The invention discloses a method for high-throughput screening of toxic substances targeting OAT1 protein, and belongs to the technical field of crossing of bioinformatics, environmental toxicology and ecological toxicology. A three-dimensional structure of the hOAT1 is constructed through homologous modeling, a small molecule compound library is constructed according to an existing chemical catalog in China, and the binding affinity of the hOAT1 and the chemicals is detected in a high-throughput manner, so that substances toxic to the hOAT1 in the compound library are obtained through screening. Compared with a traditional experimental method, the method has the advantages of high efficiency, low cost, high specificity, capability of avoiding analyzing actual blood samples with complex components and the like.

The invention belongs to the technical field of environmental toxicology and biological detection, and particularly relates to a pollutant immunotoxicity comprehensive evaluation method based on a zebra fish model. The invention aims to establish a multi-level and multi-index fused immunotoxicity evaluation system, and by combining indexes such as immune cell quantity, humoral immune factors and immune-related gene expression and constructing a comprehensive immunotoxicity index (ITI), quantitative and comprehensive judgment of pollutant immune injury is realized. Therefore, a scientific basis is provided for rapid identification and risk evaluation of immunotoxicity of environmental pollutants.

The utility model provides a zebra fish behavioral analysis device which comprises a box body, and a box cover is hinged to the box body. The interior of the box body is divided into a three-box social contact area, a novel water tank experiment area, a T labyrinth area, a Y labyrinth area and an open field area by partition plates; two first plate frames are arranged in the three-box social contact area, and through holes are formed in the first plate frames; the Y labyrinth area is provided with a Y-shaped channel defined by a second plate frame. The T-shaped labyrinth area is provided with a T-shaped channel defined by a third plate frame. Grid lines are drawn at the bottom of the open field area; grooves corresponding to the three-box social contact area, the novel air box experiment area, the T labyrinth area, the Y labyrinth area and the open field area are formed in the box cover, and cameras are arranged in the grooves; the camera is electrically connected with a touch control module arranged outside the box body, and animal behavior analysis software is arranged in the touch control module. The zebra fish behavioristics analysis device provided by the utility model can accurately record and analyze various behavioristics of the zebra fish, and further facilitates research and development in the fields of neurobiology, environmental toxicology and the like.

This invention belongs to the field of environmental toxicology and molecular detection technology, and provides a rapid and accurate method for detecting ferroptosis induced by environmental pollutants and its applications. This method utilizes early zebrafish embryos as a model organism and combines morphological, biochemical, and molecular biological indicators to establish a standardized ferroptosis detection system for identifying and quantitatively evaluating pesticide exposure-induced ferroptosis. This method features a short detection cycle (completed within 96 hours), high sensitivity, and strong specificity, effectively distinguishing between general oxidative stress and iron-dependent cell death, providing a novel detection tool for pesticide environmental toxicology research and ecological risk assessment.

The invention relates to the technical field of cell culture, in particular to an environment toxicology research cell culture device which comprises a culture box and culture dishes, and a plurality of tray assemblies used for placing the culture dishes are arranged in the culture box; a plurality of indirect boxes in one-to-one correspondence with the tray assemblies are installed at the top end of the incubator, indirect channels communicated with the interior of the incubator are formed in the inner bottom walls of the indirect boxes, and partition assemblies used for controlling the communication condition of the interiors of the indirect boxes and the interior of the incubator are installed in the indirect channels; a plurality of lifting assemblies are mounted on the inner bottom wall of the culture box; the lifting assemblies are used for lifting the culture dishes on the corresponding tray assemblies into the corresponding indirect boxes along the corresponding indirect channels. Through cooperation of the indirect box, the separation assembly and the lifting assembly, independent taking and placing of the culture dishes are achieved; during taking and placing, the indirect channel is closed, so that the incubator can be blocked from being communicated with the outside, and the conditions of temperature, humidity, CO2 concentration and other environment mutation caused by overall box opening are reduced.

The invention discloses a living cell mitochondrial H2O2 sensor based on gene coding fluorescent protein. The invention provides a recombinant cell. A preparation method of the recombinant cell comprises the following steps: (1) preparing a recombinant lentivirus for expressing a mitochondrial targeting HyPer7 probe; the mitochondrial targeting HyPer7 probe is a protein, wherein a mitochondrial targeting section and a HyPer7 probe section are sequentially arranged from an N end to a C end; and (2) transfecting THP-1 cells with the recombinant lentivirus to obtain recombinant cells. The invention also provides a macrophage obtained by inducing the recombinant cell. The recombinant cell or the macrophage can be used for H2O2-related environmental toxicology research, and has important research value and application prospect.

The invention relates to the cross technical field of environmental toxicology and computational biology, and discloses an F-53B induced intestinal flora disorder and hepatotoxicity evaluation system and method based on multi-omics conjoint analysis, and the method comprises the following steps: constructing a zebrafish-mouse cross-species exposure model of gradient dose (0-100 [mu] g / L) and multiple time points (24h / 7d / 28d), tracing by adopting a stable isotope labeling method; multiple omics data are collected, an incidence matrix is generated through algorithm fusion, a Bayesian causal network model for analyzing a causal chain and a toxicity prediction digital twinning system are constructed, a dynamic coupling relation is simulated, and an intervention effect is tested. The evaluation system comprises a plurality of modules, overcomes the defects of traditional detection, analyzes a dynamic mechanism, defines a microbiome-host interaction relationship, relieves the cross-species prediction problem, integrates multi-omics data, provides toxicity prediction and intervention guidance, can also be used for screening antagonists and drug hepatotoxicity early warning, and is wide in application prospect.

The application discloses a subtropical estuary benthic fish Oxyeleotris marmoratus AHR1b gene recombination expression plasmid and application thereof, and belongs to the technical field of environmental toxicology. The AHR1b gene recombination expression plasmid is prepared by introducing the AHR1b gene into an expression vector. A luciferase reporter gene system is also disclosed, which comprises the AHR1b gene recombination expression plasmid, an ARNT1 gene recombination expression plasmid and a firefly luciferase reporter plasmid containing a mouse CYP1A1 gene promoter. The luciferase reporter gene system is used to comprehensively characterize TCDD toxicity, to evaluate the sensitivity and specificity of the Oxyeleotris marmoratus AHR1b and ARNT1 subtype-dependent reporter gene system, to provide a theoretical basis for supporting high-throughput and high-sensitivity environmental monitoring, and to provide a species-specific early warning technology for offshore and estuary DLCs pollution.

The invention discloses an AHR2a and ARNT1 gene recombinant expression plasmid of subtropical estuary benthic fish mullet chubil and application of the recombinant expression plasmid, and belongs to the field of environmental toxicology. According to the recombinant expression plasmids, AHR2a and ARNT1 genes are respectively connected with an expression vector to construct a recombinant expression plasmid I and a recombinant expression plasmid II. The invention also constructs a luciferase reporter gene system containing the two recombinant expression plasmids and a firefly luciferase reporter plasmid containing a mouse CYP1A1 gene promoter. By utilizing the luciferase reporter gene system, the toxicity of dioxin and dioxin-like chemical substances and complex environmental wastewater can be comprehensively represented, the sensitivity and the specificity of the mugilogobius chuenensis AHR2a and ARNT1 subtype dependency reporter gene system are evaluated, and a theoretical basis is provided for supporting high-throughput and high-sensitivity environmental monitoring; and a species-specific early warning technology is provided for offshore and estuary DLCs pollution.

The invention relates to the field of environmental toxicology and developmental biology, in particular to a reproductive development toxicity evaluation method based on plastic greenhouse pesticide combined contamination. The combined action toxicity of beta-cypermethrin and emamectin benzoate is systematically evaluated from multiple dimensions of hormone level, ovarian tissue structure, placenta function, progeny development, cell viability and mitochondrial state through the design of combining an in-vivo model and in-vitro cell verification, and the potential hazard mechanism of beta-cypermethrin and emamectin benzoate in the reproductive development process is disclosed. Therefore, a scientific basis is provided for pesticide toxicology risk assessment and safety limit value formulation.

This invention relates to the fields of environmental toxicology and organoid technology, and particularly to a method for assessing the toxicity of environmental microplastic exposure based on testicular organoids. The method includes: processing waste testicular tissue into a single-cell suspension, the single-cell suspension comprising supporting cells, germ cells, and interstitial cells; seeding the single-cell suspension onto a biomimetic hydrogel scaffold for culture to obtain mature testicular organoids, which are then cultured in a target microplastic exposure solution to establish a microplastic exposure model; determining the raw values ​​of toxicity assessment indicators corresponding to the microplastic exposure model, including: morphological score data, grayscale values ​​of cell marker expression, DNA damage indicators, testosterone secretion levels, and cytotoxicity indicators; calculating a comprehensive toxicity score based on the raw values ​​of each toxicity assessment indicator, and assessing the reproductive toxicity risk level of the target microplastic exposure based on the comprehensive toxicity score. This application can improve the accuracy of assessing the reproductive toxicity risk level of microplastic exposure.

The invention belongs to the field of environmental toxicology research and application, and relates to a construction method of a physiological toxicokinetic model of organic pollutants in East Asian cowfish bodies. Comprising the following steps: determining the kinetic process of absorption, distribution, metabolism and excretion of organic pollutants in East Asian cowfish bodies; determining an atrioventricular composition structure of the physiological toxicokinetic model of the organic pollutants in the East Asian cowfish; establishing an in-vivo organic pollutant content change differential equation of the East Asian cowfish; acquiring and collecting model parameters: physiological parameters of East Asian Jiangguinea pigs, biochemical parameters of organic pollutants and toxicokinetic parameters of the organic pollutants; and solving and verifying the model. Compared with other models, the model adopts real physiological parameters of species, has higher prediction accuracy and has the potential of cross-species extrapolation among marine mammals. The model has a very wide development prospect in the field of environmental toxicology, and can gradually replace animal experiments to become an important means for ecological toxicology research of pollutants.

The invention relates to the field of biotechnology and environmental toxicology research, and particularly discloses a construction method of a GR reporter gene stably transfected cell strain based on human renal epithelial cells HEK-293T, and the construction method comprises the following steps: S1, constructing a GAL 4 / USA-ffucc-GFP-Puro reporter gene vector; s2, a GAL4DBD-GRLBD-BSD gene overexpression vector is constructed, and the GAL4DBD- And S3, carrying out lentivirus transduction and cell strain drug screening on the 293T cell, namely introducing a GAL4 / USA-ffuc-GFP-Puro reporter gene vector and a GAL4DBD-GRLBD-BSD gene overexpression vector into the cell, and carrying out screening, so as to construct the HEK-293T-GAL4 / USA-ffuc-GFP-GAL4DBD-GRLBD monoclonal stably transfected cell strain. The stably transfected cell strain can be used for evaluating the transcriptional activation or inhibition effect of an exogenous compound on a GR receptor, so that the stably transfected cell strain has important application prospects in the aspects of GR receptor function research, drug activity test, environmental endocrine disrupter (EDCs) screening and the like.

The application relates to the technical field of environment detection, and discloses an environment toxicology detection sampling device and a use method thereof, which comprises a fixing frame, the bottom of the fixing frame is fixedly connected with a large water pipe, when the environment toxicology detection sampling device is used, sampling errors may exist, an electric telescopic rod drives a sliding rod one to move through a fixed plate one, the sliding rod one drives a circular plate to move while moving, a conical telescopic rod one on the circular plate pushes a circular porous plate to slide on the sliding rod one, under the action of the conical telescopic rod one, water reflux through the holes of the circular porous plate is prevented, because half of the inside of the large water pipe is in a vacuum state, water is sucked into the inside of the large water pipe through a water suction pipe, the electric telescopic rod moves back and forth, under the action of a limiting block, the circular porous plate is pushed and reset to the original position, water is continuously pumped out, because the water suction pipe has different lengths, water of different depths is sucked, and the detection accuracy is improved.

The invention discloses a method for detecting early neurodevelopment toxicity of pollutants based on a neural crest specific molecular marker and application, and belongs to the technical field of environmental toxicology and molecular diagnosis. The method comprises the following steps: 1) exposing an early embryo to a to-be-detected sample or pollutant; 2) detecting the change of the specific molecular marker of the neural crest and the peripheral neuron; and 3) evaluating the early neurodevelopmental toxicity according to the marker expression difference. According to the method, the problems of early warning lag and low sensitivity of the traditional neural behavior endpoint (zebra fish 6dpf) are solved, the early neurotoxicity of low-concentration pollutants can be detected within 24 hours of pollutant exposure (zebra fish sox10 and prph), and the method can be used for early toxicity early warning and risk assessment in environmental monitoring and chemical risk assessment.