34 results about "Environmental toxicology" patented technology

The invention belongs to the fields of environmental toxicology and sewage biological treatment, and specifically relates to a method for improving the impact load of microorganisms in a sewage biological denitrification system against heavy metal pollution. In the sewage biological denitrification system containing heavy metal pollutants, external sources are added by An N-acyl homoserine lactone compound composed of a lactonized homoserine part and an acyl side chain with a carbon number of 6 to 14; the method of the invention has the advantages of simple operation, high processing efficiency, and a small amount of medicament can significantly improve microbial The ability to resist the impact load of heavy metals can effectively improve the effluent quality of the sewage biological treatment system under the stress of heavy metal pollution. It is economical and environmentally friendly without secondary pollution. When the sewage biological treatment system is subjected to the toxic impact of heavy metal pollutants, it can timely and effectively improve system stability and The recovery ability of the system performance can be called the sewage emergency treatment technology under the heavy metal sudden pollution accident.

The invention provides an in-vivo screening method for examining thyroid disruption and application, which relate to the technical field of environmental toxicology. The in-vivo screening method for examining thyroid disruption provided by the invention exposes xenopus laevis tadpoles at the stage of NF48 to chemical contaminant to examine the metamorphic development index of the xenopus laevis tadpoles, the in-vivo screening method for examining thyroid disruption is highly efficient and highly sensitive, the error is small, and the examination result is accurate.

The invention discloses a method for efficient transfection of turbot muscle cells. The transfection is realized by performing a nucleofection method on a constructed turbot muscle cell line serving as a material and a plasmid which is connected with a green fluorescent protein (GFP) gene which serves as an exogenous gene; the transfection efficiency is detected by a fluorescence inversion microscope and a flow cytometer. By the adoption of the transfection method disclosed by the invention, the transfection efficiency of the constructed turbot muscle cell line for the GFP gene can reach 65 percent, and cells have good growing states. The method is configured to supply a powerful tool to research the fields such as fish cell signal transduction, fish functional genomics, fish threpsology and aquatic environmental toxicology.