The invention discloses a method for identifying a plasticizer in a stilled spirit by caenorhabditis elegans, and relates to a method for indentifying the plasticizer. The method solves the problems in a conventional plasticizer detection method that the operation is complex, the dependence degree for a device is large, and the cost is high. The method comprises the steps as follows: step one, preparing a cholesterol ethanol solution; step two, preparing a phosphate buffer solution; step three, preparing culture media of elegans and step four, coating the culture media of the elegans with a colon bacillus OP50 bacterium solution uniformly, culturing the culture media for 8-12h at the temperature of 37 DEG C, putting in 8-12 sexually mature caenorhabditis elegans, culturing for 12h, and then observing the egg-laying quantity of the elegans. If the egg-laying quantity of the elegans is lower than a normal level, the fact that a distilled spirit to be detected contains the plasticizer is indicated. The detection method has low requirements for experimental conditions, and does not require any special high-precision analytical instruments or detection devices, the experiment can be operated conveniently, the cost is low, and the method can be mastered easily.