338 results about "Caenorhabditis elegans" patented technology

This invention relates generally to methods for the diagnosis and therapeutic treatment of Friedreich Ataxia. Friedreich ataxia (FRDA) is an autosomal recessive, degenerative disease that involves the central and peripheral nervous system and the heart. A gene, X25, was identified in the critical region for the FRDA locus on chromosome 9q13. The gene encodes a 210 amino acid protein, frataxin, that has homologues in distant species such as C. elegans and yeast. A few FRDA patients have been found to have point mutations in X25, but the vast majority are homozygous for a variable, unstable GAA trinucleotide expansion in the first X25 intron. Mature X25 mRNA was severely reduced in abundance in individuals with FRDA. Carriers and individuals at risk for developing FRDA can be ascertained by the methods of the present invention. Further, the methods of the present invention provide treatment to those individuals having FRDA.

Recombinant expression of fat-1 gene of Caenorhabditis elegans in a wide variety of cells, including cells of Arabidopsis thaliana and Saccharomyces cerevisiae, produces a polypeptide having ω-3 desaturase activity.

The invention relates to a method for inducing nematode-trapping fungi to synchronously produce trapping organs, which belongs to the field of applied microbiology. The method comprises the steps of culture of nematodes, preparation of a nematode extract, culture of nematode-trapping fungi and induction of trapping organs. The method can utilize the nematode extract to induce the nematode-trapping fungi to synchronously produce a large number of trapping organs (160 trapping organs / mg mycelium) by culturing the nematodes (Caenorhabditis elegans) and preparing the nematode extract by utilizing ultrasonic waves for crushing and centrifugalizing. The method has the advantages of simple and easy operation and good repeatability, and can obtain a large number of synchronous three-dimensional fungal nets, thereby providing good experimental materials for further research of the molecular mechanism of forming the trapping organs of the nematode-trapping fungi.

The invention discloses a method for carrying out toxicity analysis on tail water of a sewage treatment plant by using caenorhabditis elegans. The method comprises the following steps of (1) cultivation of the caenorhabditis elegans; (2) cultivation of escherichia coli OP 50; (3) anabiosis of the caenorhabditis elegans; (4) passage of caenorhabditis elegans; (5) synchronization of the caenorhabditis elegans; and (6) testing the comprehensive toxicity of a to-be-detected tail water sample by using the caenorhabditis elegans. The method provided by the invention has the beneficial effects that model organism-caenorhabditis elegans is utilized to carry out toxicity analysis on the tail water of the sewage treatment plant, and the size of the comprehensive toxicity of the waste water is judged by contrasting the difference of fatality rate, reproduction rate, motor behavior and generation cycle of nematodes and exposing nematodes of a blank control group.

The invention discloses an anti-aging evaluation method based on a model organism caenorhabditis elegans and an application of the method and relates to performance evaluation methods for phytochemicals, anti-aging medicines, healthcare products and the like. In the invention, under non-toxic concentration, the anti-aging effect of the to-be-tested substances is evaluated. With the evaluation method, existence of the anti-aging effect of the to-be-tested substances can be evaluated in a short period, wherein the evaluation is completed mainly through detection on life, heat stress capability,anti-ultraviolet irradiation capability, anti-oxidizing damage capability, reproductive capability, body length and lipofuscin content of the caenorhabditis elegans. The method is advantaged in that the model organism, caenorhabditis elegans, is short in growth period, so that the anti-aging effect can be observed rapidly in several weeks. The method has short experimental period and is convenientto carry out.

The invention discloses recombinant bacillus subtilis for accumulating acetylglucosamine and application thereof, and belongs to the field of genetic engineering. According to the recombinant bacillus subtilis for accumulating the acetylglucosamine and the application thereof, recombinant bacillus subtilis BSGN6-PxylA-glmS serves as an original strain, the glucosamine synthesizing route is enhanced by excessively expressing glucosamine histone acetyltransferase coding genes (CeGNA1) from caenorhabditis elegans, and the genetically engineered bacterium bacillus subtilis for accumulating the acetylglucosamine is obtained; the yield on the shake flask level reaches 7.31 g / L, and a foundation is laid for further producing the glucosamine by transforming the bacillus subtilis in metabolic engineering.

A novel small molecule antagonizes two types of acyl homoserine lactone receptors: membrane-bound and cytoplasmic. A focused library of analogs and derivatives of the original antagonist was synthesized. Analog and derivative molecules harbor a range of activities. The novel small molecule and most potent antagonist protects the eukaryote Caenorhabditis elegans from quorum-sensing-mediated killing by the bacterial pathogen Chromobacterium violaceum. The saving of C. elegans demonstrates the use of these molecules as small molecule antimicrobials.

A method of measuring generation toxicity of medicine and personal nursing products using caenorhabditis elegans, relates to a method of measuring generation toxicity of medicine and personal nursing products (PPCPs) using caenorhabditis elegans. After being contaminated on different concentration gradients and exposure time by synchronized caenorhabditis elegans, life, hatching time, generation time, body size, body corner frequency, inverse motion and olfactory stimulation of caenorhabditis elegans in parental generation P0, first filial generation F1 and second fillial generation F2 are measured, and the result is compared. These indexes characterize toxic effect of PPCPs from different sides, to estimate generation toxicity. The invention is convenient in operation and storage, little in apparatus, provides instruction for measurement and forecast for PPCPs toxicity in real environment from two sides of exposure dose and exposure time, also provides fast estimating approach for procedure of PPCPs, actual effect judgement of bursting event handling, and zoology risk of the novel PPCps.

The invention discloses an application of Chinese angelica polypeptide with effects of resisting oxidization and delaying ageing in preparation of food, particularly of health food, and further discloses a preparation method of the Chinese angelica polypeptide. The preparation method is simple and easy to implement, safe and non-toxic, stable and efficient, and is suitable for industrial production. In vitro, the Chinese angelica polypeptide has a characteristic of a certain hydroxyl radical clearance rate, which means that the Chinese angelica polypeptide has capacity of clearing active oxygen; at an integral animal level, an oxidative stress model and an ageing-associated model are used to perform a test, the test result discloses that the survival time of caenorhabditis elegans under an oxidative stress condition can be remarkably prolonged, the lipofuscin level in the caenorhabditis elegans is reduced, average lifetime of the caenorhabditis elegans is prolonged; under the oxidative stress condition and the ageing condition, the Chinese angelica polypeptide can be used for realizing remarkable anti-oxidization and ageing delaying effects by lowering the reactive oxide species (ROS) in vivo and lowering the content of lipid peroxide.

The present invention provides a robotic system for culturing and conducting experiments on C. elegans comprising a nutating tower, a well plate positioner, a reagent assembly, a liquid dispensing assembly, a wash and camera assembly, a housing assembly, a tip tray, and a three axes positioner wherein the nutating tower stores and nutates well plates. The present invention further provides a method of using the above-described system to culture C. elegans.

The invention relates to a pseudomonas syringae, a screening method thereof and application to kill nematode. The classification name of the pseudomonas syringae is pseudomonas syringae MB03, colonies has protuberances and show milk white, the thalline is in the shape of a rod through microscopic examination and belongs to a gram-negative bacterium. The screening method comprises: acquiring frozen plant tissue, chopping and culturing in a medium, and finally picking growing single colony, and performing separation purification through streaking. The application to kill nematode means that the thalline, a fermentation supernatant, a cell disruption solution, or extracellular crude protein and / or intracellular crude protein of pseudomonas syringae MB03 subjected to ammonium sulfate precipitation is used to kill caenorhabditis elegans and meloidogyne incognita. The advantages comprise that pseudomonas syringae is low in production cost and free of pollution to environment, and has relatively killing effect on caenorhabditis elegans and meloidogyne incognita. The factor, killing nematode, in pseudomonas syringae is protein, so that pseudomonas syringae is sutiable for molecular gene operation, and has great value on microbe control resource and mechanism research.

The invention relates to a method for identifying waste oil by using Caenorhabditis elegans dormancy as a biological marker, and relates to a waste oil identifying method. The method of the invention solves the problems of low sensitivity, poor accuracy, complex operation and high cost existed in the current waste oil identification method. The method comprises the following steps: 1, uniformly mixing NaCl, agar powder, peptone, a CaCl2 aqueous solution, a MgSO4 aqueous solution, a phosphate buffer and distilled water to obtain a nutrient solution, then sterilizing, and drying to obtain a medium; and 2) coating escherichia coli OP50 bacterial liquid in a prepared medium in the step 1), culturing for certain time, and culturing Caenorhabditis elegans larva for 46 hours. The method has the following advantages that 1, effective identification of high refining waste oil with low specific component trace can be realized; and 2, the operation is convenient, the cost is low, the method is easy to train and grasp. The method of the invention is mainly used for identifying the waste oil.

The invention relates to a construction method of a caenorhabditis elegans hyperuricemia model. The method comprises the following steps that 1, the uric acid content in caenorhabditis elegans is measured through a high performance liquid chromatography method; 2, an optimal drug delivery and culture mode is determined; 3, an optical drug delivery substance and drug delivery dosage are determined; 4, the optimal drug delivery time of xanthine is determined; 5, series experiment evaluation is conducted on the caenorhabditis elegans hyperuricemia model obtained through the construction method, and the caenorhabditis elegans hyperuricemia model which is low in damage, efficient, stable and reliable is obtained. According to the construction method, the hyperuricemia animal model is constructed by means of the model organism caenorhabditis elegans which has the high conservative property to human genes and has the similar uric acid metabolic pathway with human beings, the model has typical pathological characterization and is stable and reliable, various quantity indexes have the statistical significance, and a powerful tool is provided for large-scale and high-throughput drug screening of gout and the hyperuricemia symptom.

The invention discloses a chalcone derivative containing 1, 1-dichloropropene, a preparation method and application thereof. The derivative has a general formula (I) shown as the specification, wherein R1 is selected from 4-chlorphenyl, 4-fluorophenyl, benzyl, 4-chlorobenzyl and other substituents; R2 is selected from methyl, ethyl, benzyl, 4-chlorobenzyl, ethyl acetate and other substituents. The derivative provided by the invention has the characteristics of simple structure, simple preparation process and low production cost, and has good activity to rice bacterial leaf blight, rice baeterial leaf streak pathogens and caenorhabditis elegans.

The invention relates to a water extraction method of ricinine, application of extract and a toxicity evaluation method of ricinine. Magnetic-force heating and mixing auxiliary ultrasonic extraction technology is adopted, water is used as extraction solvent, the method of extracting high-purity ricinine from castorseed shells and castor old leaves is utilized, ricinine is used for controlling grain storing pests such as sitophilus zeamais and tribolium castaneum, caenorhabditis elegans are used for toxicity evaluation of ricinine, and lethality, fecundity, body length and activity of enzyme inside body of caenorhabditis elegans are determined. The water extraction method of ricinine, application of extract and the toxicity evaluation method of ricinine are simple in operation and low in cost, reduce use of organic reagents, protect the environment, and provide necessary conditions for large-scale industrial production of ricinine biological pesticides due to the fact that extraction solution can be directly used for controlling grain storing pests.

The invention discloses a method for identifying a plasticizer in a stilled spirit by caenorhabditis elegans, and relates to a method for indentifying the plasticizer. The method solves the problems in a conventional plasticizer detection method that the operation is complex, the dependence degree for a device is large, and the cost is high. The method comprises the steps as follows: step one, preparing a cholesterol ethanol solution; step two, preparing a phosphate buffer solution; step three, preparing culture media of elegans and step four, coating the culture media of the elegans with a colon bacillus OP50 bacterium solution uniformly, culturing the culture media for 8-12h at the temperature of 37 DEG C, putting in 8-12 sexually mature caenorhabditis elegans, culturing for 12h, and then observing the egg-laying quantity of the elegans. If the egg-laying quantity of the elegans is lower than a normal level, the fact that a distilled spirit to be detected contains the plasticizer is indicated. The detection method has low requirements for experimental conditions, and does not require any special high-precision analytical instruments or detection devices, the experiment can be operated conveniently, the cost is low, and the method can be mastered easily.

The invention discloses a micro-fluidic electrical impedance detecting and sorting chip for Caenorhabditis elegans. The chip comprises a substrate, a micro-fluidic channel layer and a gas cavity layerwhich are sequentially stacked from bottom to top, a pair of spaced coplanar microelectrodes is arranged on the substrate, the micro-fluidic channel layer comprises a nematode micro-channel positioned at the upstream, a target nematode channel and a remaining nematode channel which are positioned at the bifurcated downstream, a lower nematode suspension inlet, a lower target nematode sample outlet and a lower remaining nematode sample outlet, and the gas cavity layer comprises a target cavity, a remaining cavity, an upper nematode suspension inlet, an upper target nematode sample outlet and an upper remaining nematode sample outlet. The coplanar microelectrodes are arranged in the micro-fluidic electrical impedance detecting and sorting chip to detect nematode samples to be sorted, and the on-off of a compressed air introduced to the target cavity and the remaining cavity is controlled to realize the sorting of the target nematode, so the sorting chip can achieve high throughput and automation, and can effectively guarantee the detection efficiency.

The present invention relates to methods and compositions for high content drug screening in C. elegans which may be used to identify compounds that treat disorders associated with protein aggregation.

The invention relates to streptomyces lateritius A001 and application thereof. The streptomyces lateritius A001 separated from the soil is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.21851. A fermentation supernatant and a thallus extract of the strain have a relatively good anti-aging effect on caenorhabditis elegans, and can be used for assisting in preparing anti-aging products, so that the strain is applied to relieving aging and prolonging the service life, has a good practical application value, and provides an excellent basic strain for further research and development of microorganism-derived anti-aging products.

The invention discloses a method for detecting persistent toxicity of soluble heavy metals by using caenorhabditis elegans, relating to a method of detecting the toxic effect of the soluble heavy metals under the conditions of long term and low concentration by carrying out the persistent exposure covering multiple successive generations of soluble heavy metals through adopting caenorhabditis elegans. In the invention, the egg initiation concentration which does not affect the normal egg-hatch and is in the presence without heavy metals, and the food initiation concentration which can meet the needs of growth and development of eggs are determined; toxicity transmission strength and transmission paths and the like of the heavy metals under the long-term actual envrionemnt exposure are obtained through mutual comparison of same indexes among different generations under the condition that the exposure time, and the exposure doses, particularly the coexistence of foods and heavy metals and other aspects stay closer to the actual environmental exposure condition. The invention makes up the deficiency of the traditional method for testing toxicity by using caenorhabditis elegans, has aclear pertinence, and supplements and extends the toxicity research method which is under the condition of simulating actual environmental exposure.

The invention discloses a method for screening a drug which is resistant to pan-drug-resistant acinetobacter baumannii by using caenorhabditis elegans. First, a caenorhabditis elegans-pan-drug-resistant acinetobacter baumannii infection model used for screening the efficacy of a composition is constructed, wherein caenorhabditis elegans has double gene mutation of glp-4 and sek-1, the culture medium of the caenorhabditis elegans-pan-drug-resistant acinetobacter baumannii co-culture consists of 20% of BHI, 5-20[mu]m of nalidixic acid and 5-20[mu]g / ml of FeCl3; the concentration of the pan-drug-resistant acinetobacter baumannii is 1*10<6>-1*10<9>CFU / mL; the duration time of the bacterial infection on the caenorhabditis elegans is 6-12 hours; and the time of the treatment on an infected modelby a drug is 24-48 hours. The model can be used for fast and high-throughput screening of in-vivo antibacterial activity of various compounds or drugs or compositions, and compared with an in-vivo animal infection model, the model has the huge advantages of low preparation cost, short period and easy operation. Compared with an in vitro model, the model can be used to screen out compounds which have large in-vivo toxicity, poor metabolism and low in vitro-in vivo correlation.

The invention relates to a multifunctional chemical crosslinking agent. The multifunctional chemical crosslinking agent is a three-arm type structure consisting of two reaction functional group arms and an arm containing an affine functional group and a cleavage site. The invention further provides a preparation method of the multifunctional chemical crosslinking agent. The multifunctional crosslinking agent is very excellent in both of water solubility and stability, and high in crosslinking efficiency; when the multifunctional crosslinking agent is applied to protein analysis; its good crosslinking peptide fragment identification ability is presented in samples of simple systems such as BSA, and ten standard protein mixtures; the crosslinking agent also can identify considerate crosslinking site in complex biological samples such as escherichia coli 70S ribosome, escherichia coli full cell lysis buffer and caenorhabditis elegans full cell lysis buffer, and present very good application value; thereby providing a powerful tool for the future study of interaction of complex biological system proteins.

The invention provides a caenorhabditis elegans capture system and a caenorhabditis elegans capture method. The caenorhabditis elegans capture system comprises a caenorhabditis elegans location device, a caenorhabditis elegans capture device, and a control device. During caenorhabditis elegans capture, a digital microscope acquires image information of an observed caenorhabditis elegans in a caenorhabditis elegans culture dish and transmits the image information to the control terminal, the control terminal outputs a control instruction according to the image information and controls a 3D motion mechanism through a control panel to adjust the relative positions of the digital microscope and the caenorhabditis elegans culture dish so as to enable caenorhabditis elegans location image information meeting the image identification requirement to be obtained through the digital microscope, and the caenorhabditis elegans capture device captures the located caenorhabditis elegans under control according to the caenorhabditis elegans location information. Compared with the prior art, the caenorhabditis elegans capture system and the caenorhabditis elegans capture method of the invention have the advantages of quick and accurate location, efficient caenorhabditis elegans capture and the like.

The invention relates to volatile compound methyl thiobutyrate and application thereof, and belongs to the technical field of a microbial biological pesticide. The volatile compound methyl thiobutyrate is detected by the following steps that a, the used bacterial strain is achromobacter (Achoromobacter sp.) CD-253, and the preservation number is CGMCC No6358; b, cultivating a CD-253 bacterial strain in an LB culture medium by using a conventional fermentation method, and carrying out headspace extraction on a fermentation liquor by using a solid-phase microextraction technology; c, carrying out gas chromatography-mass spectrometer (GC-MS) analysis on an extracting matter; and d, carrying out comparison of a national Institute of standards and Technology (NIST) database to obtain volatile metabolite methyl thiobutyrate. The volatile compound methyl thiobutyrate has inhibiting effects on caenorhabditis elegans (Caenorhabditis elegans) and meloidogyne incognita (Meloidogyne incognita), and can be applied to preparation of a vermifuge preparation and a precusor substance. Compared with the traditional nematicides pesticide, the volatile compound methyl thiobutyrate has the advantages of no environment pollution, low cost and excellent nematode killing effect.

The invention provides a method for detecting the toxicity of an antibacterial agent. The method comprises the steps that caenorhabditis elegans is exposed in a to-be-detected sample, and according tothe body length, the body bending frequency, the head swing frequency, the offspring number, the incubation time, the generation time and / or the population scale of the caenorhabditis elegans, the toxicity of the antibacterial agent to growth and development, neural systems and / or reproductive systems is evaluated, wherein the to-be-detected sample contains the antibacterial agent. Compared withthe prior art, the method for detecting the toxicity of the antibacterial agent, provided by the invention, is rapid and efficient, has a short experiment period and few requirements on an instrument,and can estimate a safety application range of the antibacterial agent in daily care products; and meanwhile, the detection method provided by the invention provides a technical basis for speculatingthe influence of the antibacterial agent on offspring, and the influence of the antibacterial agent on parental nematodes and offspring of the parental nematodes can be obtained.

The invention discloses a bacterial Lysobacter soli 6262 fermentation liquid and application thereof. The bacterial Lysobacter soli 6262 fermentation liquid is obtained through bacterial strain screening and Lysobacter soli 6262 fermentation liquid preparation. The bacterial strain Lysobacter soli 6262 is preserved at the General Microbiology Center of the China Committee for Culture Collection of Microorganisms on January 09, 2017 and the preservation number is CGMCC N0.13555. The bacterial Lysobacter soli 6262 fermentation liquid is applied to preparation of a biological agent for killing meloidogyne incognita (Meloidogyne incognita) and caenorhabditis elegans (Caenorhabditis elegans). The bacterial Lysobacter soli 6262 fermentation liquid has the beneficial effects of being high in efficiency, low in toxicity and free of environmental pollution, can be used for preparing a biological pesticide preparation for killing the meloidogyne incognita (M.incognita) and the caenorhabditis elegans (C.elegans).

The invention belongs to the field of biotechnology and specifically discloses a garlic antimicrobial peptide AR117 and its application. The applicant builds a garlic cDNA library and finally isolatesthe garlic antimicrobial peptide AR117. The garlic antimicrobial peptide AR117 has a sequence shown in the formula of SEQ ID NO. 2. The applicant finds that the AR117 has a strong inhibitory effect on the growth of tomato canker, bacterial wilt, wheat seedlings and phytophthora capsici, which are difficult to be controlled by chemical drugs, can also inhibit bacillus cereus causing food poisoning, abdominal pain, vomiting and diarrhea and bacillus anthracis causing human anthracnose, and can effectively influence caenorhabditis elegans feeding. The hemolysis experiment result shows that the gene can inhibit nematodes and does not damage the mammals and human body. The antibacterial peptide gene is screened from garlic and can be used for biological control in the prevention and control ofplant diseases and insect pests in the later research, or can be used as an antibacterial and anti-nematode drug.

The invention discloses a biological metabonomics analysis method for screening antioxidant active substances, and belongs to the technical field of metabonomics analysis. Metabolites of caenorhabditis elegans samples are detected and analyzed by adopting a GCTOF / MS technology. ChromaTOF software and MS DIAL software carry out data conversion, calibration of a data baseline, peak alignment, identification, peak area calculation and the like, and normalize the peak area. Multivariate statistical analysis on five groups of caenorhabditis elegans samples including a normal control group, a modelgroup and three anthocyanin pretreatment groups are carried out, and biological metabolites with remarkable differences are screened. 15, 8, 9 and 14 different metabolites with antioxidant action mechanisms are respectively obtained through screening and checking, a model group and three anthocyanin pretreatment groups, recognition and high-throughput analysis of the antioxidant action mechanismsare realized by utilizing callback change of abundance of the different metabolites, and a metabolite path is visualized.