105 results about "Caenorhabditis elegans" patented technology

The invention relates to the technical field of biological detection, and discloses a caenorhabditis elegans reproductive toxicity intelligent evaluation system and an application method.The caenorhabditis elegans reproductive toxicity intelligent evaluation system comprises a standardized exposure and imaging module used for executing a high-flux pollutant exposure experiment under a controlled environment condition and synchronously collecting nematode behavior and gonad image data; and the multi-modal data acquisition unit is connected to the standardized exposure and imaging module and is used for extracting and preprocessing behavioral parameters and reproductive toxicity indexes from the acquired images. According to the method, 12 behavior parameters and 8 core reproductive indexes are automatically extracted by constructing a multi-modal data acquisition unit and utilizing an improved U-Net model and a specific algorithm, so that full-process automation from image acquisition to key index quantification is realized. The problems of low efficiency, large subjective deviation and poor repeatability caused by dependence on manual interpretation in the prior art are solved, and the objectivity of the evaluation result and the high-throughput screening efficiency are remarkably improved.

The invention relates to the technical field of chemical toxicology, in particular to an evaluation method for comprehensively analyzing the immune induction effect of a plasticizer by using caenorhabditis elegans, according to the evaluation method, a mother worm breeding mode is changed to replace a lysis solution to synchronize nematodes, experimental interference is reduced, the result accuracy is improved, monitoring indexes are newly increased, intestinal injuries and in-vivo bacterial indexes are increased, and the evaluation method is suitable for comprehensive analysis of the immune induction effect of the plasticizer. The evaluation is more accurate and reliable by combining oxidative stress indexes and performing two-dimensional comprehensive evaluation from an intestinal immune defense line and oxidative stress, and by drawing a multi-index monitoring shape graph, adopting a comprehensive biomarker response index analysis method and associating immune-related representative indexes, the evaluation accuracy is improved. According to the method, the change of each index and the immunity intensity on the caenorhabditis elegans under different exposure conditions are intuitively presented, the immune defense effect of the caenorhabditis elegans under the stress of the plasticizer can be quickly and effectively evaluated without transcriptome and metabonomics, and efficient evaluation is realized.

The invention relates to an uncaria alkaloid extract as well as a preparation method and application thereof. The method comprises the following steps: heating and leaching uncaria stem and branch powder with acidic ethanol to obtain an acid alcohol leaching solution; carrying out reduced pressure distillation and concentration on the leach liquor to obtain an uncaria total alkaloid extract; removing acid-insoluble substances from the total alkaloid extract, degreasing, adjusting alkali, extracting with chloroform, and recovering a chloroform layer to obtain uncaria crude alkaloid; and dissolving the uncaria crude alkaloid in distilled water, adding into a macroporous resin chromatographic column, adsorbing, flushing, eluting by using a 20% ethanol solution with five column volumes, and collecting the eluent to obtain the uncaria alkaloid extract. The ramulus uncariae cum uncis alkaloid extract PURA1 has the effects of improving the pathological phenotype of the Alzheimer's disease model caenorhabditis elegans and improving the oxidative stress resistance of the caenorhabditis elegans, plays a role in prolonging the service life of the caenorhabditis elegans by regulating and controlling the p38MAPK signal channel of the caenorhabditis elegans, and can be used for preparing the medicine for relieving the Alzheimer's disease and resisting aging.

The invention relates to the technical field of biological detection, and discloses a metabonomics-based caenorhabditis elegans compound pollutant exposure evaluation method which comprises the following specific steps: S1, constructing a compound pollutant exposure system; s2, carrying out nematode culture and exposure treatment; s3, extracting in-vivo metabolites of the nematodes; s4, carrying out metabonomics analysis; s5, carrying out bioinformatics analysis; and S6, verifying the combined toxicity effect. By establishing the metabonomics-based caenorhabditis elegans compound pollutant exposure evaluation method, the limitation of a traditional single pollutant evaluation system is broken through, and systematic and high-sensitivity detection of multi-pollutant combined toxicity is realized; in addition, by constructing a standardized composite exposure system and an efficient metabolite extraction process and combining multi-dimensional mass spectrum data and bioinformatics modeling, a synergistic, antagonistic or additive effect mechanism among pollutants is deeply analyzed from the molecular level.

This invention discloses a method for screening key neurotoxic substances in food raw materials and their products using a *C. elegans* model combined with metabolomics. Food samples are processed to prepare a stock solution, which is then used to cultivate *C. elegans*. The sample exhibiting the greatest neurotoxicity to the nematodes is identified by analyzing the number of *C. elegans* movements and neuronal fluorescence images. Subsequently, key neurotoxic substances in food are systematically screened using a combination of non-targeted liquid chromatography-tandem mass spectrometry (LC-MS / MS) and a metabolomics platform. This application establishes a novel method for screening neurotoxic substances by combining nematode activity with non-targeted LC-MS / MS determination and a non-targeted metabolic platform, clarifying key neurotoxic substances in food and providing a theoretical basis for food quality control and high-quality development.

The invention relates to the field of microorganisms and application, in particular to brevibacillus mat, a microbial agent containing the brevibacillus mat and application of the brevibacillus mat. The invention provides a brevibacillus sschleri strain KY987 which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the brevibacillus sschleri strain KY987 is CGMCC No. 37625. The strain not only has the activity of killing main pathogenic bacteria of soybean root rot, but also has broad-spectrum bactericidal activity on various plant pathogenic bacteria. In addition, the strain also has caenorhabditis elegans killing activity, is easy to grow, and is suitable for realizing large-scale industrial application.

The invention belongs to the technical field of cosmetic detection, and relates to a method for evaluating the skin barrier repair effect of cosmetics and application of the method, and the method comprises the following steps: carrying out grouping culture on nematodes after the same period: a culture of a sample group contains a to-be-detected sample; the culture of the control group does not contain the sample to be detected, and other culture conditions are consistent with those of the sample group; the cultured nematodes are collected, nematode precipitates are centrifugally taken and dyed, the dyed nematodes are subjected to fluorescence intensity detection, and if the fluorescence intensity of the nematodes in the sample group is remarkably different from that of the nematodes in the control group, the sample to be detected has the skin barrier repairing effect. The method for evaluating the repairing effect of the cosmetic is provided on the basis of caenorhabditis elegans, the repairing degree of the product to the caenorhabditis elegans is judged by measuring the fluorescence intensity of the caenorhabditis elegans, and then the repairing effect of the product is reflected. The experimental period is short, the experimental consumable cost is low, and ethical conflicts do not exist.

The application discloses Bifidobacterium Pseudocatenulatum and application thereof, and the Bifidobacterium Pseudocatenulatum YSBP01CCTCC NO: M 20232067. Experiments prove that the Bifidobacterium Pseudocatenulatum can enhance the antioxidant capacity of Caenorhabditis elegans, prolong the life of the nematode, improve the activity and the reproductive capacity of the nematode, reduce fat accumulation, improve the mitochondrial membrane potential, and delay the aging of the body. The Bifidobacterium Pseudocatenulatum can also significantly inhibit the weight increase of a high-fat-diet mouse, reduce the TG level in serum of the mouse, significantly reduce the fasting blood glucose concentration, and effectively prevent and relieve obesity and type 2 diabetes.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to application of an ephedra-apricot kernel cough-relieving traditional Chinese medicine composition in preparation of a medicine for treating Parkinson's disease, the ephedra-apricot kernel cough-relieving traditional Chinese medicine composition provided by the invention shows a remarkable treatment effect on Parkinson's disease in a caenorhabditis elegans Parkinson's disease pathological model, and the medicine composition can be used for remarkably treating the Parkinson's disease. The polypeptide has the potential of treating the Parkinson's disease and can be applied to preparation of drugs for treating the Parkinson's disease.

The invention discloses wheat malt seedling functional dietary fibers as well as a preparation method and application of the wheat malt seedling functional dietary fibers. The soluble dietary fiber and the insoluble dietary fiber of the wheat malt seedlings are extracted by adopting a wall breaking-extraction mode of synergy of biological enzyme and free radicals. The wheat malt seedling soluble dietary fibers can prolong the life of caenorhabditis elegans in an oxidative stress state, improve the antioxidant enzyme activity and improve the anti-apoptosis capability; the intestinal flora of the obese C57BL / 6J mouse induced by the high-fat feed is regulated; the wheat malt seedling insoluble dietary fiber has better binding water capacity, water-holding capacity and cholesterol and sodium cholate adsorption capacity, and can be used as a functional food additive for improving the texture and nutritional characteristics of food; and the wheat malt seedlings can also be used for assisting in reducing serum cholesterol level, preventing arteriosclerosis, promoting defecation, preventing constipation, colorectal cancer and the like in the field of health medical treatment, so that high-quality reutilization of the wheat malt seedlings is realized.

The application discloses application of leucyl phenylalanine in preparation of a medicine for Parkinson's disease. Specifically, it is found that leucyl phenylalanine can relieve dopamine neuron dendrite degeneration of a Caenorhabditis elegans model of Parkinson's disease, enhance autophagy level, and improve proteasome activity, thereby providing an effective treatment scheme for treating Parkinson's disease.

The invention discloses application of a caenorhabditis elegans reproductive disorder model to screening of strains promoting reproduction health, and relates to the technical field of biologica.Cyclophosphamide medicine intervention is applied to caenorhabditis elegans, so that the normal reproductive system of caenorhabditis elegans is damaged, and the reproductive disorder model is prepared; then 3985 gene knockout strains in an escherichia coli Keio gene knockout library are utilized, and strains with the reproductive capacity remarkably improved for reproductive disorder are screened; and carrying out homologous gene knockout on the probiotics by using a gene editing technology, and screening out the probiotics with the potential of promoting reproductive health. The reproductive disorder model is stable and repeatable, can be used for screening strains or drugs for improving the reproductive function, and also can be used for screening candidate strains or drugs for reducing the reproductive toxicity of cyclophosphamide. According to the method, an effective tool is provided for screening of reproduction-related probiotic strains or screening of related drugs, and meanwhile, the strains screened based on the model provide new candidate strains for treatment and research of reproductive disorder diseases.

The utility model discloses a track type caenorhabditis elegans space biology experiment device, which relates to the technical field of caenorhabditis elegans and comprises an experiment main body, and a temperature controller is fixedly connected to the center of the back of the outer wall of the experiment main body. The limiting boxes are arranged at the four corners of the inner wall of the experiment body, and the first electric lead screws are arranged on the inner walls of the limiting boxes, so that vertical movement of the supporting frame is controlled, the output end of the sprayer can be close to a culture medium, and part of a culture solution is prevented from being sprayed outside; a second electric screw rod is started, and a moving block arranged on the outer wall of the second electric screw rod is controlled, so that the moving block controls the longitudinal movement of the culture table through the screw rod, and the problems that when caenorhabditis elegans is subjected to a culture experiment, nematode groups in a culture medium are usually fed in a manual dripping mode, and when a large number of experiment samples are faced, the caenorhabditis elegans cannot be fed are solved. And a large amount of time is often consumed, so that the experiment efficiency is relatively low.

The invention belongs to the technical field of medicines, and particularly relates to application of vitamin B12 in preparation of a medicine for treating retinitis pigmentosa. Specifically, based on the high conservative property of human PRPF31 and caenorhabditis elegans prp-31 genes, a prp-31 gene knock-down and mutation nematode disease model is constructed, and the model can reproduce multiple pathological phenotypes such as oxidative stress, lysosome dysfunction and apoptosis caused by PRPF31 defects. Experiments prove that the active form (such as mecobalamin) of the vitamin B12 can actively pass through the blood retina barrier by virtue of an endogenous transport mechanism through oral administration, the pathological damage can be reversed by virtue of multi-channel synergy, and the pathological phenotype of a model organism can be remarkably improved, so that the vitamin B12 has a good practical application value.

The invention discloses a germplasm creation method of hermetia illucens rich in highly unsaturated fatty acids, and belongs to the technical field of insect genetic breeding and aquatic feed raw material development. The fat-1 gene of caenorhabditis elegans is introduced into a hermetia illucens genome through a transgenic technology, a fat-1 gene transfer type hermetia illucens strain is obtained, the HUFA content in the hermetia illucens of the strain is remarkably improved compared with that of a wild type, and particularly, the content of DPA (22: 5n-3), DHA (22: 6n-3) and other essential fatty acids for the human body is remarkably increased. The method can be used for creating excellent germplasm resources of hermetia illucens, and provides an effective technical approach and germplasm resources for developing novel functional aquatic feed and relieving fish meal and fish oil resource pressure.

The invention belongs to the technical field of biological detection, and particularly discloses a pair of echinococcosis detection markers based on serum circulating miRNA and application of the echinococcosis detection markers, the detection markers are a combination of miR-192-5p and miR-122-5p, the abundance of the miR-192-5p and the miR-122-5p in the serum of an echinococcosis patient is remarkably reduced, the miR-192-5p and the miR-122-5p are in negative correlation with echinococcosis infection, and the threshold values are 0.35 and 0.04 respectively. During detection, the relative expression level (taking caenorhabditis elegans miR-39-3p as an external reference) of caenorhabditis elegans miR-39-3p in serum is determined through qPCR, and the positive and negative properties are judged according to a threshold value; the area under the ROC curve of the marker combination reaches 0.97, and a double-blind test shows that the positive rate and the negative rate of human and mouse serum detection are 100%. The method solves the problems of missed diagnosis and misdiagnosis in existing echinococcosis detection, can be used for preparing a detection kit, realizes accurate detection, curative effect evaluation and prognosis management of echinococcosis, and has extremely high clinical application value.

The invention discloses a simple device for disinfecting and cooling a caenorhabditis elegans scalpel. The simple device comprises a bottom bracket mechanism, three alcohol lamps, three overhanging supporting mechanisms and three elastic clamping mechanisms, the three alcohol lamps are respectively placed at three heating point positions on the bottom bracket mechanism and are positioned at different heights; the three overhanging supporting mechanisms are all rotationally installed on the bottom support mechanism and located at different height positions. The three elastic clamping mechanisms are fixed to the overhanging ends of the three overhanging supporting mechanisms correspondingly and used for elastically clamping the surgical blade. According to the simple device for disinfecting and cooling the scalpel, through cooperation of the three overhanging supporting mechanisms and the three elastic clamping mechanisms, stable clamping of the three scalpel blades can be achieved, manual handheld heating is not needed, and therefore the stability of heating and disinfecting of the scalpel blades is guaranteed, and the safety in the operation process can also be guaranteed.

The invention relates to the technical field of biological detection and toxicological evaluation, and discloses a neural behavior multi-parameter integration-based caenorhabditis elegans exposure evaluation method which comprises the following specific steps: S1, synchronous culture of caenorhabditis elegans; s2, pollutant exposure treatment; s3, multi-parameter behavior detection; s4, data standardization processing; and S5, constructing a comprehensive evaluation model, and performing weighted integration on four core parameters including the movement speed, the chemotactic index, the synaptic spot density and the calcium signal amplitude after standardization to construct a neurotoxicity index NTI. According to the method disclosed by the invention, a multi-layer and multi-dimensional neurotoxicity evaluation system is constructed by integrating four types of neurobehavior parameters including motion behaviors, chemotactic reactions, synaptic morphology and neuron calcium signals, and the defect that a traditional single endpoint index cannot comprehensively reflect overall disturbance of a neural regulation network is overcome; by utilizing the high homology of the nervous system of caenorhabditis elegans and the human nerve gene, the clinical correlation and mechanism analysis capability of environmental pollutant neurotoxicity evaluation are remarkably improved.

The present invention relates to an animal model for oxidative stress research and use thereof, and more specifically, the present invention can utilize a mutant of RCAT having a regulatory function for an antioxidant stress regulator in Caenorhabditis elegans and a human cell line expressing RCAT as animal and human cell line models for oxidative stress research, using the mutant and the human cell line.

The invention discloses an acanthopanax senticosus-polysaccharide protein compound as well as a preparation method and application thereof, and belongs to the technical field of biology. According to the preparation method of the acanthopanax senticosus-proteoglycan protein compound, acanthopanax senticosus is taken as a raw material, and the acanthopanax senticosus-proteoglycan protein compound is extracted by adopting a microwave-enzyme synergistic extraction method. The acanthopanax senticosus-polysaccharide protein compound disclosed by the invention can inhibit Abeta-induced apoptosis in CL4176 caenorhabditis elegans, and shows remarkable effects in the aspects of prolonging the service life, reducing the paralysis rate, improving the athletic ability, enhancing antioxidant defense and the like.

The invention discloses laurel essential oil with anti-aging activity and application of the laurel essential oil. According to the method, laurel essential oil is used for acting on caenorhabditis elegans to verify the aging delaying effect of the caenorhabditis elegans, after the caenorhabditis elegans are counted and subjected to synchronization treatment, a life determination test of the caenorhabditis elegans is carried out, a blank control group, a DMSO solvent group, a positive drug group and a laurel essential oil experimental group are set respectively, a survival curve is drawn, and statistical analysis is carried out. Results show that the lifetime of nematodes in a laurel essential oil treatment group is remarkably prolonged, and the anti-aging effect of the laurel essential oil treatment group is superior to that of a Meanwhile, target function analysis on the laurel essential oil shows that the essential oil regulates and controls senescence-related pathways. The further determination of the effect of monomer components in the laurel essential oil on delaying caenorhabditis elegans aging shows that p-cymene, methyleugenol, ocimene, linalool, sabinene, 4-terpene alcohol, terpinene and linalyl acetate show significant anti-aging activity.

The invention discloses an anti-aging drug screening method based on metabonomics. Firstly, a group of biomarkers, including L-valine, 4-hydroxyproline, L-serine, L-leucine, L-proline and melatonin, which are closely related to the caenorhabditis elegans aging process are identified in the invention. On the basis, the invention provides a drug screening method which comprises the following steps: performing metabonomics analysis on the nematodes subjected to drug administration treatment, detecting the content change of the biomarker group, and if the content of the biomarker group has obvious callback (up-regulation) compared with that of an old control group, judging that the drug has a potential anti-aging effect. The technical defects that a traditional nematode life experiment is long in period, tedious in operation, low in flux, high in subjectivity and the like are overcome, and a new anti-aging drug screening strategy which is rapid, stable and efficient and can preliminarily reveal drug effects is provided.

The invention discloses a system and a method for measuring motion information of caenorhabditis elegans based on speckle images. The system comprises a bearing assembly, a laser assembly is arranged on one side of the bearing assembly, and an imaging assembly is arranged on one side, away from the laser assembly, of the bearing assembly; the laser assembly transmits the bearing assembly in the first direction to provide a light source for the imaging assembly. Or the laser assembly and the imaging assembly are located on the same side of the bearing assembly; the laser assembly irradiates the bearing assembly in the second direction to provide a light source for the imaging assembly. The imaging assembly obtains a plurality of original speckle images of the caenorhabditis elegans and transmits the original speckle images to the processing assembly; according to the measurement system provided by the invention, the original speckle image of the caenorhabditis elegans is directly obtained, analysis is carried out according to the original speckle image, the contrast map is determined, the motion information of the caenorhabditis elegans is determined according to the contrast map, and on the premise that the caenorhabditis elegans does not need to be transferred, the measurement accuracy of the caenorhabditis elegans is improved. And rapid and objective evaluation of nematode motion information is realized.

The present application relates to the technical field of medicine, and provides application of strong loquat juice in preparation of a medicine for improving and / or treating muscle atrophy. The strong loquat juice is applied in preparation of the medicine for improving and / or treating muscle atrophy, in particular age-related muscle atrophy or inflammation factor-induced muscle atrophy, and the strong loquat juice improves and / or treats muscle atrophy by improving motor function, promoting myotube differentiation and hypertrophy, and maintaining muscle fiber structural integrity. The present application discloses the improvement effect of the strong loquat juice on muscle structure and function in cell and animal models: in a TNF-alpha-induced C2C12 myotube atrophy model, the strong loquat juice can significantly increase myotube diameter and improve myosin heavy chain expression; in an aging-related Caenorhabditis elegans model, the strong loquat juice can significantly enhance body bending ability, pharyngeal pump movement rate and muscle fiber structural integrity.

The invention provides a novel application of quercetin-3-O-beta-D-glucuronide, belongs to the technical field of medicines, and particularly relates to an application of the quercetin-3-O-beta-D-glucuronide in preparation of anti-aging medicines. According to the invention, quercetin-3-O-beta-D-glucuronide is extracted and separated from lotus leaves, and quercetin-3-O-beta-D-glucuronide can obviously prolong the service life of caenorhabditis elegans and has the effect of delaying senescence, so that quercetin-3-O-beta-D-glucuronide has the potential of delaying senescence, and can be used for preparing a medicine for treating caenorhabditis elegans. The traditional Chinese medicine composition can be applied to preparation of anti-aging medicines and also can be applied to preparation of health-care products for delaying aging.

The present application belongs to the technical field of bioactive polysaccharides, and particularly relates to astragalus polysaccharide for improving gait, and a preparation method and application thereof. The astragalus polysaccharide is prepared by hot water extraction, and low-molecular astragalus polysaccharide is prepared by degrading the astragalus polysaccharide with weak acid. Further, the obtained astragalus polysaccharide and low-molecular astragalus polysaccharide are taken as objects, and behavioral study is carried out on Caenorhabditis elegans as a model, and the turning ratio of Caenorhabditis elegans is taken as a gait detection index, so as to confirm that the astragalus polysaccharide and low-molecular astragalus polysaccharide both have the effect of improving gait. The astragalus polysaccharide and low-molecular astragalus polysaccharide provided by the present application can be further used for the development of food, medicine and health products, and has a wide application prospect.

The invention discloses a Hannaea yeast ZAFU HH1, which is classified and named as Hannaea zeae, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 28, 2023, and the preservation number is CGMCC No.27725. The Hannaea yeast ZAFU HH1 is named as Hannaea zeae and is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 28, 2023. The invention also discloses a microbial inoculum prepared from the strain. The invention also discloses application of the bacillus subtilis and a microbial agent prepared from the bacillus subtilis in conversion of zearalenone. The yeast Hannai ZAFU HH1 has excellent ZEN conversion capability, the conversion product is identified as a derivative with lower toxicity, and the safety of the ZEN conversion product is evaluated by using caenorhabditis elegans as a model organism, so that the conversion product is proved to have good biological safety. In addition, under the conditions of low temperature stress and acid stress, the yeast Hannai ZAFU HH1 still keeps a high ZEN conversion rate.

The invention discloses an automatic device for disinfecting and cooling a caenorhabditis elegans scalpel. The automatic device comprises a control box, a supporting bottom plate, a supporting stand column, two alcohol lamps, a rotary driving mechanism, an air cooling mechanism and four blade clamping mechanisms. The supporting stand column is rotationally and vertically installed in the center of the supporting bottom plate. The four blade clamping mechanisms are installed at the upper ends of the supporting stand columns. The two alcohol lamps are arranged at the two heating points; the rotation driving mechanism is used for driving the supporting stand column to rotate. And the air cooling mechanism is arranged at the cooling point. According to the automatic device for disinfecting and cooling the scalpel, the supporting stand column can be rotationally driven, so that the point positions where the four blade clamping mechanisms are located are switched at regular time, and finally, the heated and disinfected scalpel blade is automatically fed into the air cooling mechanism to be rapidly cooled, so that the disinfecting efficiency of the scalpel blade is improved; and the scalding danger possibly caused by manual handheld heating can be avoided, and automatic heating and cooling of the surgical blade are achieved.

The invention belongs to the technical field of biology, and discloses an application of M6 peptide in enhancing the oxidation resistance of cells, caenorhabditis elegans and mice. The invention discloses application of M6 peptide or pharmaceutically acceptable salt thereof in antioxidation or preparation of antioxidation products. The amino acid sequence of the M6 peptide is shown as SEQ ID NO: 1. The M6 peptide can enhance the oxidation resistance of cells and relieve H2O2-induced cell apoptosis; the antioxidant capacity of the caenorhabditis elegans can be enhanced, oxidative damage is relieved, and the service life of the caenorhabditis elegans is prolonged; and in-vivo oxidative damage, induced by D-galactose, of mice can be relieved by enhancing the activity of CAT and SOD (superoxide dismutase). The M6 peptide is expected to become a novel medicine for treating oxidative damage related diseases and delaying the oxidative damage process.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to application of active ingredients of cistanche deserticola to preparation of a medicine for treating Alzheimer's disease. The active ingredients of the cistanche deserticola 70% ethanol extract, the cistanche deserticola water extract and the cistanche deserticola glycoside A provided by the invention show a remarkable treatment effect on the Alzheimer's disease in a caenorhabditis elegans Alzheimer's disease pathological model; it is found that the cistanche deserticola water extract, the cistanche deserticola 70% ethanol extract and the cistanche deserticola glycoside A can remarkably delay the paralysis rate of the paralysis phenotype of caenorhabditis elegans with the Alzheimer's disease, have the potential of treating the Alzheimer's disease and can be applied to preparation of the medicine for treating the Alzheimer's disease.