165 results about "Caenorhabditis elegans" patented technology

The invention discloses sturgeon ovary active peptide with ovary aging resistance and a preparation method of the sturgeon ovary active peptide. According to the invention, sturgeon ovary is taken as a raw material, and peptide with the highest ovarian aging resistance activity is selected from different enzymolysis combinations through an in-vitro anti-oxidation test and an ovarian granular cell and caenorhabditis elegans test; three sturgeon ovary active peptide sequences with ovary senescence resistance activity are screened by virtue of bioinformatics technologies such as mass spectrum identification, molecular docking and the like, and the amino acid sequences of the sturgeon ovary active peptide sequences are Phe-Met-Ile (FMI), Phe-Asp-Pro-Phe (FDPF) and Ser-Trp-Pro-Trp-Gln (SWPWQ). The sturgeon ovarian peptide prepared by the method not only has remarkable anti-premature ovarian failure activity and successfully solves the problem of high-valued utilization of sturgeon byproducts, but also can be used as a natural and efficient functional factor, is widely applied to functional products in the related fields of health care products, cosmetics, medicines and the like, and has a wide application prospect. And the method shows extremely wide market application prospects and huge economic values.

The embodiment of the invention discloses a sleep peptide and differential metabolite detection method for caenorhabditis elegans, belongs to the technical field of organism metabolism, and aims to research sleep-awakening key metabolite of caenorhabditis elegans and a regulation mechanism of the sleep-awakening key metabolite. According to the sleep peptide for caenorhabditis elegans, the sleep peptide is an F4 monomer as shown in SEQ ID NO: 1. Compared with the prior art, the sleep and awakening process of the caenorhabditis elegans under the intervention of the goat milk-derived sleep peptide is analyzed through a metabonomics technology, the key metabolite for regulating and controlling sleep and awakening is successfully screened out, and a core metabolic pathway related to the key metabolite is disclosed. The application also constructs a metabolite-pathway-target regulation framework, fills the blank of sleep research in the aspect of metabolic regulation, provides a scientific basis for a sleep intervention strategy based on metabolic regulation, and also lays a foundation for developing metabolic markers of sleep states in the future.

The invention relates to the technical field of environmental health risk evaluation, in particular to a method for evaluating the risk of Parkinson's disease induced by environmental pollutants. The method for evaluating the risk of inducing the Parkinson's disease by the environmental pollutants comprises the following steps: carrying out a subacute exposure experiment by taking caenorhabditis elegans as a model organism; observing the transport capacity of the caenorhabditis elegans, observing and counting the abnormal movement ability of the caenorhabditis elegans, and determining the lipofuscin level of the caenorhabditis elegans, the damage degree of dopaminergic neurons, the dopamine level and the expression and aggregation conditions of alpha-synuclein protein, so as to evaluate the risk of the Parkinson's disease induced by environmental pollutants; the environmental pollutant is 6: 2 chlorinated polyfluoroalkyl ether sulfonate. According to the method, the PD toxicity evaluation model is generated by constructing a behavior characteristic and molecular characteristic scheme and combining multi-strain nematode data, and the problem that in the prior art, the risk of inducing PD by environmental pollutants is difficult to comprehensively reveal due to the fact that a single dimension is concerned is solved.

The invention discloses a method for increasing the extraction yield of tremella polysaccharide and application, dry tremella is used as a raw material, tremella polysaccharide is effectively extracted by utilizing an ultrasonic-cellulase wall breaking coupling green solvent method, the extraction yield of tremella polysaccharide is effectively increased, the method is green and environment-friendly and is easy to control, and the obtained tremella polysaccharide has excellent biological activity and can be used for preparing the tremella polysaccharide. The water loss rate of water can be reduced, and the moisturizing and anti-wrinkle capabilities are good; the caenorhabditis elegans can effectively remove hydroxyl free radicals, DPPH free radicals and ABTS free radicals, shows strong oxidation resistance, can significantly prolong the survival rate of caenorhabditis elegans under hydrogen peroxide stress, and shows that the caenorhabditis elegans has good oxidative stress resistance.

The invention discloses lactobacillus johnsonii A21065 capable of resisting oxidation, prolonging life and delaying Parkinson's disease as well as a product and application of the lactobacillus johnsonii A21065, and belongs to the technical field of functional strains. The preservation number of the lactobacillus johnsonii A21065 is GDMCC (China General Microbiological Culture Collection Center) No: 65767. In a caenorhabditis elegans model, the lactobacillus johnsonii A21065 shows that the lactobacillus johnsonii A21065 has better effects of improving the oxidation resistance of nematodes, resisting environmental stress and prolonging the life, also shows a good effect in in-vitro experiments of resisting artificial gastrointestinal fluid, resisting cholate and the like, meanwhile, the lactobacillus johnsonii A21065 also has an effect of improving the cognition of a Parkinson nematode model, and the lactobacillus johnsonii A21065 has a good application prospect. The invention has important significance for preventing and / or treating Parkinson's disease.

The invention provides a crocodile source Aeromonas daka argH gene deletion strain, a crocodile source Aeromonas daka argH gene back-filling strain and a construction method and application of the crocodile source Aeromonas daka argH gene deletion strain and the crocodile source Aeromonas daka argH gene back-filling strain, an argH gene recombinant suicide plasmid pRE112-delta argH is constructed by adopting a seamless cloning method, an Aeromonas daka strain is jointed with E.coli WM3064 containing the pRE112-delta argH to obtain the argH gene deletion strain delta argH, and a corresponding gene back-filling strain C delta argH is further constructed. Through biological characteristic analysis, the argH gene deletion reduces synthesis of arginine of Aeromonas dakara and biofilm formation amount, reduces growth rate, proteolytic activity and resistance to crocodile-derived antibacterial peptide, but enhances hemolytic activity, and significantly improves the survival rate of Caenorhabditis elegans, so that the Aeromonas dakara has a good application prospect in treatment of caenorhabditis elegans, and the Aeromonas dakara has a good application prospect in treatment of caenorhabditis elegans. Important reference is provided for further researching the regulation effect of the argH gene in a bacterial pathogenic mechanism and developing related prevention and control strategies.

The invention relates to the technical field of biological detection, and discloses a caenorhabditis elegans reproductive toxicity intelligent evaluation system and an application method.The caenorhabditis elegans reproductive toxicity intelligent evaluation system comprises a standardized exposure and imaging module used for executing a high-flux pollutant exposure experiment under a controlled environment condition and synchronously collecting nematode behavior and gonad image data; and the multi-modal data acquisition unit is connected to the standardized exposure and imaging module and is used for extracting and preprocessing behavioral parameters and reproductive toxicity indexes from the acquired images. According to the method, 12 behavior parameters and 8 core reproductive indexes are automatically extracted by constructing a multi-modal data acquisition unit and utilizing an improved U-Net model and a specific algorithm, so that full-process automation from image acquisition to key index quantification is realized. The problems of low efficiency, large subjective deviation and poor repeatability caused by dependence on manual interpretation in the prior art are solved, and the objectivity of the evaluation result and the high-throughput screening efficiency are remarkably improved.

The invention relates to the technical field of chemical toxicology, in particular to an evaluation method for comprehensively analyzing the immune induction effect of a plasticizer by using caenorhabditis elegans, according to the evaluation method, a mother worm breeding mode is changed to replace a lysis solution to synchronize nematodes, experimental interference is reduced, the result accuracy is improved, monitoring indexes are newly increased, intestinal injuries and in-vivo bacterial indexes are increased, and the evaluation method is suitable for comprehensive analysis of the immune induction effect of the plasticizer. The evaluation is more accurate and reliable by combining oxidative stress indexes and performing two-dimensional comprehensive evaluation from an intestinal immune defense line and oxidative stress, and by drawing a multi-index monitoring shape graph, adopting a comprehensive biomarker response index analysis method and associating immune-related representative indexes, the evaluation accuracy is improved. According to the method, the change of each index and the immunity intensity on the caenorhabditis elegans under different exposure conditions are intuitively presented, the immune defense effect of the caenorhabditis elegans under the stress of the plasticizer can be quickly and effectively evaluated without transcriptome and metabonomics, and efficient evaluation is realized.

The invention relates to an uncaria alkaloid extract as well as a preparation method and application thereof. The method comprises the following steps: heating and leaching uncaria stem and branch powder with acidic ethanol to obtain an acid alcohol leaching solution; carrying out reduced pressure distillation and concentration on the leach liquor to obtain an uncaria total alkaloid extract; removing acid-insoluble substances from the total alkaloid extract, degreasing, adjusting alkali, extracting with chloroform, and recovering a chloroform layer to obtain uncaria crude alkaloid; and dissolving the uncaria crude alkaloid in distilled water, adding into a macroporous resin chromatographic column, adsorbing, flushing, eluting by using a 20% ethanol solution with five column volumes, and collecting the eluent to obtain the uncaria alkaloid extract. The ramulus uncariae cum uncis alkaloid extract PURA1 has the effects of improving the pathological phenotype of the Alzheimer's disease model caenorhabditis elegans and improving the oxidative stress resistance of the caenorhabditis elegans, plays a role in prolonging the service life of the caenorhabditis elegans by regulating and controlling the p38MAPK signal channel of the caenorhabditis elegans, and can be used for preparing the medicine for relieving the Alzheimer's disease and resisting aging.

The invention belongs to the technical field of preparation and application of natural products, and particularly relates to a perilla frutescens extract product for activating mitochondrial autophagy and resisting aging and application of the perilla frutescens extract product. Perillaldehyde is obtained by purifying perilla leaves, and the purity reaches 99%. The compound has DPPH free radical scavenging capacity and tyrosinase activity inhibition capacity, and the DPPH free radical scavenging capacity is equivalent to that of vitamin E; the service life of the high-concentration perillaldehyde group is prolonged by 8%; the service life of caenorhabditis elegans under the environments of thermal stimulation, ultraviolet stimulation and oxide stimulation is remarkably prolonged, and the adversity service life prolonging rate of a high-concentration perillaldehyde group is at least 20%; sOD (superoxide dismutase) and CAT (catalase) activity of caenorhabditis elegans is remarkably improved, H2O2 accumulation is reduced, and anti-stress capability is improved; the caenorhabditis elegans mitochondrial autophagy is promoted, the mitochondrial autophagy pathway related genes are adjusted, the mitochondrial function is improved, the NAD < + > / NADH ratio is increased, the body metabolism is maintained, and the aging is delayed; the perillaldehyde capable of promoting the mitochondrial autophagy pathway can be applied to development of anti-aging drugs, health care products, food, cosmetics and other products.

The invention discloses a method for preparing alkyl glycoside by using caenorhabditis elegans, and belongs to the technical field of biochemical engineering. The alkyl glycoside with the carbon chain length of C7-C15 series is extracted from caenorhabditis elegans for the first time, and the specific preparation steps are as follows: culturing caenorhabditis elegans by using an oat culture medium; the method comprises the following steps: ultrasonically crushing caenorhabditis elegans, freezing with liquid nitrogen, and freeze-drying at an ultralow temperature; extracting the dry powder by using an extracting agent; carrying out rotary evaporation on the obtained extract to obtain a solid substance, redissolving the solid substance with methanol, standing, centrifuging after standing, taking a supernatant, and repeating the steps of standing and centrifuging until no precipitate appears in the supernatant to obtain a precipitate-free supernatant; and filtering the obtained precipitate-free supernatant by using a filter head, and then carrying out LC-MS detection to obtain different alkyl glycosides. The C7-C15 series alkyl glycoside is synchronously obtained through nematode metabolism regulation and a multi-mode separation process, nematode biological metabolites and a green extraction agent are adopted in the whole process, and toxic by-products are avoided.

The invention relates to the technical field of biological detection, and discloses a metabonomics-based caenorhabditis elegans compound pollutant exposure evaluation method which comprises the following specific steps: S1, constructing a compound pollutant exposure system; s2, carrying out nematode culture and exposure treatment; s3, extracting in-vivo metabolites of the nematodes; s4, carrying out metabonomics analysis; s5, carrying out bioinformatics analysis; and S6, verifying the combined toxicity effect. By establishing the metabonomics-based caenorhabditis elegans compound pollutant exposure evaluation method, the limitation of a traditional single pollutant evaluation system is broken through, and systematic and high-sensitivity detection of multi-pollutant combined toxicity is realized; in addition, by constructing a standardized composite exposure system and an efficient metabolite extraction process and combining multi-dimensional mass spectrum data and bioinformatics modeling, a synergistic, antagonistic or additive effect mechanism among pollutants is deeply analyzed from the molecular level.

This invention discloses a method for screening key neurotoxic substances in food raw materials and their products using a *C. elegans* model combined with metabolomics. Food samples are processed to prepare a stock solution, which is then used to cultivate *C. elegans*. The sample exhibiting the greatest neurotoxicity to the nematodes is identified by analyzing the number of *C. elegans* movements and neuronal fluorescence images. Subsequently, key neurotoxic substances in food are systematically screened using a combination of non-targeted liquid chromatography-tandem mass spectrometry (LC-MS / MS) and a metabolomics platform. This application establishes a novel method for screening neurotoxic substances by combining nematode activity with non-targeted LC-MS / MS determination and a non-targeted metabolic platform, clarifying key neurotoxic substances in food and providing a theoretical basis for food quality control and high-quality development.

The invention relates to the field of microorganisms and application, in particular to brevibacillus mat, a microbial agent containing the brevibacillus mat and application of the brevibacillus mat. The invention provides a brevibacillus sschleri strain KY987 which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the brevibacillus sschleri strain KY987 is CGMCC No. 37625. The strain not only has the activity of killing main pathogenic bacteria of soybean root rot, but also has broad-spectrum bactericidal activity on various plant pathogenic bacteria. In addition, the strain also has caenorhabditis elegans killing activity, is easy to grow, and is suitable for realizing large-scale industrial application.

The application relates to the field of biotechnology, in particular to the application of Caenorhabditis elegans in constructing an animal model for detecting combined toxicity of antibiotics and metal ions. The application provides the application of Caenorhabditis elegans in constructing an animal model for detecting combined toxicity of antibiotics and metal ions. The application takes Caenorhabditis elegans as a model organism, and provides a simple, fast, low-cost, short-cycle and reproducible method for constructing an animal model for detecting combined toxicity. The application provides technical support for combined toxicity of antibiotics and metal ions in a natural environment and specific action aspects, and has practical application value.

The invention belongs to the technical field of cosmetic detection, and relates to a method for evaluating the skin barrier repair effect of cosmetics and application of the method, and the method comprises the following steps: carrying out grouping culture on nematodes after the same period: a culture of a sample group contains a to-be-detected sample; the culture of the control group does not contain the sample to be detected, and other culture conditions are consistent with those of the sample group; the cultured nematodes are collected, nematode precipitates are centrifugally taken and dyed, the dyed nematodes are subjected to fluorescence intensity detection, and if the fluorescence intensity of the nematodes in the sample group is remarkably different from that of the nematodes in the control group, the sample to be detected has the skin barrier repairing effect. The method for evaluating the repairing effect of the cosmetic is provided on the basis of caenorhabditis elegans, the repairing degree of the product to the caenorhabditis elegans is judged by measuring the fluorescence intensity of the caenorhabditis elegans, and then the repairing effect of the product is reflected. The experimental period is short, the experimental consumable cost is low, and ethical conflicts do not exist.

The present invention discloses a method for preparing a polysaccharide from Acanthopanax senticosus medicinal residue that reduces Aβ deposition in Caenorhabditis elegans. The method comprises the following steps: using waste generated in the elegans glycoside extraction process as a raw material, drying and crushing the waste, adding water and stirring evenly, subjecting the waste to dynamic high-pressure microfluidization homogenization, adding a surfactant to the treated liquid for ultrasonic extraction, centrifuging the obtained polysaccharide extract to obtain the supernatant, concentrating the supernatant under reduced pressure, and precipitating the polysaccharide from the Acanthopanax medicinal residue with alcohol. The present invention uses Acanthopanax medicinal residue as a raw material, recovers and reuses the active ingredients in the Acanthopanax medicinal residue, and promotes the sustainable development of the Acanthopanax medicinal residue industry. The preparation method disclosed in the present invention has the advantages of being easy to operate, time-saving, efficient, and pollution-free. The Acanthopanax medicinal residue polysaccharide prepared by the present invention has the efficacy of reducing Aβ deposition in Caenorhabditis elegans.

The application discloses Bifidobacterium Pseudocatenulatum and application thereof, and the Bifidobacterium Pseudocatenulatum YSBP01CCTCC NO: M 20232067. Experiments prove that the Bifidobacterium Pseudocatenulatum can enhance the antioxidant capacity of Caenorhabditis elegans, prolong the life of the nematode, improve the activity and the reproductive capacity of the nematode, reduce fat accumulation, improve the mitochondrial membrane potential, and delay the aging of the body. The Bifidobacterium Pseudocatenulatum can also significantly inhibit the weight increase of a high-fat-diet mouse, reduce the TG level in serum of the mouse, significantly reduce the fasting blood glucose concentration, and effectively prevent and relieve obesity and type 2 diabetes.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to application of an ephedra-apricot kernel cough-relieving traditional Chinese medicine composition in preparation of a medicine for treating Parkinson's disease, the ephedra-apricot kernel cough-relieving traditional Chinese medicine composition provided by the invention shows a remarkable treatment effect on Parkinson's disease in a caenorhabditis elegans Parkinson's disease pathological model, and the medicine composition can be used for remarkably treating the Parkinson's disease. The polypeptide has the potential of treating the Parkinson's disease and can be applied to preparation of drugs for treating the Parkinson's disease.

The invention relates to the technical field of biological control, and discloses an application of steviosin or a substance containing the steviosin in preparation of a nematode control product. According to a caenorhabditis elegans toxicity tracking method, stevia rebaudiana mother liquor sugar is separated according to the polarity of the substance; two compounds are separated and purified from mother liquor sugar by using normal phase silica gel, reverse phase silica gel, LH20 gel and liquid chromatography in sequence, the compounds are identified by using mass spectrum and nuclear magnetism, the compounds are determined to be Steretin F and Steretin E, the Steretin F and the Steretin E are proved to have nematicidal toxicity, the Steretin F has the strongest toxicity, LC50 is 1.57, the toxicity effect is stronger than that of thiazoline and weaker than that of abamectin, and the Steretin F and the Steretin E have the nematicidal toxicity. A new direction is provided for application of stevia rebaudiana, and an important theoretical and practical basis is provided for development and large-scale application of natural insecticidal compounds.

The invention discloses wheat malt seedling functional dietary fibers as well as a preparation method and application of the wheat malt seedling functional dietary fibers. The soluble dietary fiber and the insoluble dietary fiber of the wheat malt seedlings are extracted by adopting a wall breaking-extraction mode of synergy of biological enzyme and free radicals. The wheat malt seedling soluble dietary fibers can prolong the life of caenorhabditis elegans in an oxidative stress state, improve the antioxidant enzyme activity and improve the anti-apoptosis capability; the intestinal flora of the obese C57BL / 6J mouse induced by the high-fat feed is regulated; the wheat malt seedling insoluble dietary fiber has better binding water capacity, water-holding capacity and cholesterol and sodium cholate adsorption capacity, and can be used as a functional food additive for improving the texture and nutritional characteristics of food; and the wheat malt seedlings can also be used for assisting in reducing serum cholesterol level, preventing arteriosclerosis, promoting defecation, preventing constipation, colorectal cancer and the like in the field of health medical treatment, so that high-quality reutilization of the wheat malt seedlings is realized.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a poppy seed oil, shiny-leaved yellowhorn oil and cistanche water extract composition for treating Alzheimer's disease and application of the poppy seed oil, shiny-leaved yellowhorn oil and cistanche water extract composition. The poppy seed oil, shiny-leaved yellowhorn oil and cistanche water extract composition provided by the invention shows a remarkable treatment effect on the Alzheimer's disease in a caenorhabditis elegans obesity pathological model, can remarkably reduce the paralysis proportion of human-derived A beta protein transgenic caenorhabditis elegans, has the potential of treating the Alzheimer's disease, and can be used for treating the Alzheimer's disease. The compound can be applied to preparation of medicines for treating the Alzheimer's disease, and has a wide application prospect.

The invention discloses an application of a tetrahydroindazole derivative in preparation of a medicine for delaying senescence and prolonging life, the tetrahydroindazole derivative is specifically N-(4-(pyridine-4-) phenyl)-N '-(4, 5, 6, 7-tetrahydro-1H-indazole-5-) oxamide, and the structural formula of the tetrahydroindazole derivative is shown as a formula I. The invention further discloses a preparation method of the tetrahydroindazole derivative. By investigating the influence of N-(4-(pyridine-4-) phenyl)-N '-(4, 5, 6, 7-tetrahydro-1H-indazole-5-) oxamide on the life of caenorhabditis elegans, the obtained experimental result shows that N-(4-(pyridine-4-) phenyl)-N'-(4, 5, 6, 7-tetrahydro-1H-indazole-5-) oxamide has relatively strong anti-aging activity, and can effectively prolong the life of caenorhabditis elegans. Therefore, the compound is expected to be used in preparation and development of medicines for delaying senescence and prolonging life.

The invention provides application of arachidonic acid in delaying reproductive aging, and particularly relates to the technical field of reproductive health. The invention provides application of arachidonic acid in preparation of a product for delaying reproductive aging and / or prolonging the reproductive period. By taking caenorhabditis elegans and mice as examples, the arachidonic acid can obviously activate reproductive aging gonad lysosome, protect oocyte quality and prolong reproductive cycle, thereby delaying reproductive aging. The invention provides a new application prospect of arachidonic acid in the aspects of delaying ovarian aging and protecting fertility.

The application discloses application of leucyl phenylalanine in preparation of a medicine for Parkinson's disease. Specifically, it is found that leucyl phenylalanine can relieve dopamine neuron dendrite degeneration of a Caenorhabditis elegans model of Parkinson's disease, enhance autophagy level, and improve proteasome activity, thereby providing an effective treatment scheme for treating Parkinson's disease.

The invention discloses application of a caenorhabditis elegans reproductive disorder model to screening of strains promoting reproduction health, and relates to the technical field of biologica.Cyclophosphamide medicine intervention is applied to caenorhabditis elegans, so that the normal reproductive system of caenorhabditis elegans is damaged, and the reproductive disorder model is prepared; then 3985 gene knockout strains in an escherichia coli Keio gene knockout library are utilized, and strains with the reproductive capacity remarkably improved for reproductive disorder are screened; and carrying out homologous gene knockout on the probiotics by using a gene editing technology, and screening out the probiotics with the potential of promoting reproductive health. The reproductive disorder model is stable and repeatable, can be used for screening strains or drugs for improving the reproductive function, and also can be used for screening candidate strains or drugs for reducing the reproductive toxicity of cyclophosphamide. According to the method, an effective tool is provided for screening of reproduction-related probiotic strains or screening of related drugs, and meanwhile, the strains screened based on the model provide new candidate strains for treatment and research of reproductive disorder diseases.

The present invention belongs to the technical field of organic chemical synthesis, and specifically relates to a rhein nitroxide derivative, a synthesis method, and an application thereof. The present invention uses rhein, an antioxidant pharmacological substance from the traditional Chinese medicine rhubarb, as a research object, selects its carboxyl group at position 3 as a modification site, and introduces four active intermediate nitroxides into the rhein structure based on the amide condensation and esterification reaction mechanisms and the pharmacophore splicing principle. Thirty rhein nitroxide derivatives were designed and synthesized, thereby establishing a method for synthesizing novel rhein nitroxide derivatives with mild reaction conditions, environmental friendliness, and high yield. The provided novel rhein nitroxide derivatives have the ability to scavenge DPPH and ABTS free radicals, have a protective effect on oxidatively damaged cells, and have the ability to delay the lifespan of Caenorhabditis elegans. Their effects are superior to those of rhein, providing a new approach for the development of antioxidant and anti-aging drugs.

The utility model discloses a track type caenorhabditis elegans space biology experiment device, which relates to the technical field of caenorhabditis elegans and comprises an experiment main body, and a temperature controller is fixedly connected to the center of the back of the outer wall of the experiment main body. The limiting boxes are arranged at the four corners of the inner wall of the experiment body, and the first electric lead screws are arranged on the inner walls of the limiting boxes, so that vertical movement of the supporting frame is controlled, the output end of the sprayer can be close to a culture medium, and part of a culture solution is prevented from being sprayed outside; a second electric screw rod is started, and a moving block arranged on the outer wall of the second electric screw rod is controlled, so that the moving block controls the longitudinal movement of the culture table through the screw rod, and the problems that when caenorhabditis elegans is subjected to a culture experiment, nematode groups in a culture medium are usually fed in a manual dripping mode, and when a large number of experiment samples are faced, the caenorhabditis elegans cannot be fed are solved. And a large amount of time is often consumed, so that the experiment efficiency is relatively low.

The invention belongs to the technical field of medicines, and particularly relates to application of vitamin B12 in preparation of a medicine for treating retinitis pigmentosa. Specifically, based on the high conservative property of human PRPF31 and caenorhabditis elegans prp-31 genes, a prp-31 gene knock-down and mutation nematode disease model is constructed, and the model can reproduce multiple pathological phenotypes such as oxidative stress, lysosome dysfunction and apoptosis caused by PRPF31 defects. Experiments prove that the active form (such as mecobalamin) of the vitamin B12 can actively pass through the blood retina barrier by virtue of an endogenous transport mechanism through oral administration, the pathological damage can be reversed by virtue of multi-channel synergy, and the pathological phenotype of a model organism can be remarkably improved, so that the vitamin B12 has a good practical application value.

The invention discloses a germplasm creation method of hermetia illucens rich in highly unsaturated fatty acids, and belongs to the technical field of insect genetic breeding and aquatic feed raw material development. The fat-1 gene of caenorhabditis elegans is introduced into a hermetia illucens genome through a transgenic technology, a fat-1 gene transfer type hermetia illucens strain is obtained, the HUFA content in the hermetia illucens of the strain is remarkably improved compared with that of a wild type, and particularly, the content of DPA (22: 5n-3), DHA (22: 6n-3) and other essential fatty acids for the human body is remarkably increased. The method can be used for creating excellent germplasm resources of hermetia illucens, and provides an effective technical approach and germplasm resources for developing novel functional aquatic feed and relieving fish meal and fish oil resource pressure.