94 results about "Lactobacillus johnsonii" patented technology

The present invention demonstrated the potential use of Lactobacillus johnsonii D115 as a probiotic, as a prophylactic agent or as a surface treatment of materials against human and animal pathogens such as Brachyspira pilosicoli, Brachyspira hyodysenteriae, Shigella sonnei, Vibrio cholera, Vibrio parahaemolyticus, Campylobacter jejuni, Streptococcus pneumoniae, Enterococcus faecalis, Enterococcus faecium, Clostridium perfringens, Yersinia enterocolitica, Escherichia coli, Klebbsiella pneumoniae, Staphylococcus aureus, Salmonella spp., Bacillus cereus, Aspergillus niger and Fusarium chlamydosporum. The proteineous antimicrobial compound was partially characterized and found to be heat tolerant up to 121° C. for 15 min, and acid tolerant up to pH1 for 30 min at 40° C. The compound is also stable to enzymatic digestion, being able to retain more than 60% antimicrobial activity when treated with pepsin and trypsin.

The invention provides a slimming enzyme and a preparation method thereof. The preparation method comprises the following steps: (1), preparing and pretreating materials in proportion; (2), aerobically fermenting the treated materials via high-sugar Angel yeast; (3), after fermentation with the yeast, aerobically fermenting with acetic acid bacteria; (4), after fermentation with the acetic acid bacteria, anaerobically fermenting with probiotics; (5), after fermentation with the probiotics, fermenting at low temperature and filtering to obtain the finished enzyme. The product produced by the above method has slight vinegar aroma and sour and sweet taste, is not less than 1046 U / ml in lipase activity and not less than 6.8*108 in the count of live Lactobacillus johnsonii and has significant slimming effect.

The invention discloses a probiotics-Chinese herbal medicine compound composition capable of loosening bowels to relieve constipation and application of the probiotics-Chinese herbal medicine compoundcomposition and belongs to the technical field of health-care foods. The composition comprises 13-15 kinds of lactobacillus rhamnosus, lactobacillus plantarum, lactobacillus paracasei, bifidobacterium lactis, lactobacillus acidophilus, bifidobacterium longum, lactobacillus reuteri, lactobacillus helveticus, lactobacillus fermentum, lactobacillus johnsonii, lactobacillus casei, lactobacillus crispatus, bifidobacterium breve, bifidobacterium bifidum and bifidobacterium infantis, 10-13 kinds of isomalto-oligosaccharide, inulin, lactitol, stachyose, fructo-oligosaccharide, xylo-oligosaccharide, Mannan-oligosaccharide, galacto-oligosaccharide, oat beta-glucan, glucan, soluble soybean polysaccharide, resistant dextrin and chitosan-oligosaccharide and 2-4 kinds of malts, fructus crataegi, blackplums and fermented soybeans. According to the probiotics-Chinese herbal medicine compound composition and the application thereof, probiotics, prebiotics and traditional Chinese medicine powder are compounded and can jointly promote intestinal canal health.

The invention relates to the technical field of food enzymes, and discloses a symbiotic microflora enzyme. The symbiotic microflora enzyme contains symbiotic microflora which has the effect of preventing, resisting and inhibiting helicobacter pylori. The symbiotic microflora contains main microflora and auxiliary microflora which aim at enhancing microflora effects and increasing the microflora number, wherein the main microflora comprises lactobacillus acidophilus, lactobacillus bulgaricus, lactobacillus plantarum, lactobacillus rhamnosus, Roy's lactobacillus, lactobacillus casei, lactobacillus gasseri, lactobacillus johnsonii, lactobacillus salivarius, lactobacillus brevis, lactic streptococci, bacillus subtilis, bifidobacterium bifidum, bifidobacterium adolescentis, bifidobacterium infantis, bifidobacterium breve and bifidobacterium longum. After the symbiotic microflora enzyme is drunk, the contained powerful and rich symbiotic microflora can naturally exist in the digestive system of the human body, obtains the living space advantage in the ecological environment, and achieves the effect of eliminating helicobacter pylori.

The invention discloses a bacillus coagulans strain capable of resisting streptococcus and applications of the bacillus coagulans strain. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and is assigned with the accession number of CGMCC No.12196. The strain is prepared into fungus powder which is added to an animal feed, the fungus powder also can beapplied as a microbial preparation for resisting streptococcus, and after the feed containing the strain is used for feeding tilapia mossambica, streptococcicosis can be effectively prevented and treated. The strain is mixed with bacillus subtilis and lactobacillus johnsonii, then a compound microbial preparation is prepared, and the compound microbial preparation can improve the growth performance of tilapia mossambica, and improve the activity of superoxide dismutase and catalase of the liver and lysozyme in serum. In addition, the fermentation broth of the strain can be adopted as a breeding environment modifier, after the fermentation liquor of the strain is sprayed to an aquaculture pond of tilapia mossambica, the quantity of streptococcus in the aquaculture water can be reduced, andnitrite in the water can be eliminated under the low oxygen condition.

The invention discloses a Lactobacillus johnsonii, and a preparation and application thereof, and discloses an optimal culture medium for the strain and a preparation method of a freeze-dried preparation of the strain. The microbial preservation number of the Lactobacillus johnsonii ZLJ010 is CGMCC No.5762. The strain is definite in hereditary basis, and has the characteristics of good growth performance and high acid production, acid resistance, heat resistance and bacterium inhibition capabilities. When being used as a feed additive for sows, the freeze-dried preparation of the strain can improve the intestinal tract health of the sows, reduce the number of harmful bacteria in feces, enhance the body immunity and increase the production performance of the sows.

A biological fermentation pig forage is prepared from the following raw materials in parts by weight: 45-53 parts of corn flour, 8-10 parts of herb of common nipplewort, 3-4 parts of konjac glucomannan, 3-4 parts of glucosamine sulfate, 35-40 parts of soybean meal, 35-40 parts of barley vinasse, 17-25 parts of wheat bran, 10-13 parts of euglena, 3-4 parts of spora lygodii, 1-1.5 parts of lactobacillus fermentum, 1-1.5 parts of aspergillus niger, 1.5-2 parts of lactobacillus plantaum, 1.5-2 parts of lactobacillus johnsonii, 0.2-0.3 part of sodium selenosulfate, 3-4 parts of vitamin e acetate, and 10-12 parts of illite powder. The biological fermentation pig forage is improved in digestion rate, and is capable of increasing digestion capability of animals on the forage and improving animal growth performance, especially is capable of enhancing immunity, possesses protrude effects on reducing pig cold and mycoplasma pneumoniae of swine, and is capable of improving pork tenderness and reducing muscle shearing force.

A method for administering viable microorganism compositions for poultry, comprising: administering a viable microorganism composition comprising viable lactic acid bacteria belonging to Lactobacillus reuteri and Lactobacillus johnsonii at the stage of newborn fledgling; and administering a viable microorganism composition which comprises a viable microorganism belonging to Bacillus subtilis.

The invention belongs to the technical field of biology, and in particular relates to a protective agent for vacuum freeze drying of lactobacillus johnsonii, and application thereof. The protective agent provided by the invention contains no sorbitol substances, has lower cost, can provide effective protection for lactobacillus johnsonii in a vacuum freeze drying process, and is favorable to improvement of the utilization efficiency of a lactobacillus microbial feed additive, the protective rate of the protective agent is high, and the yield of produced live bacteria is above 109.

The invention discloses a multi-joint bacterium lacticum composition. The multi-joint bacterium lacticum composition is an advantage bacterial strain selected by a separating screen in the vagina of ahealthy female in China and at least comprises three active components of Lactobacillus johnsonii (Lactobacillus johnsonii), Lactobacillusgasseri (Lactobacillusgasseri), Lactobacillus rhamnosus ( Lactobacillus rhamnosus ), Lactobacillus crispatus ( Lactobacillus crispatus ) and Lactobacillus jensenii (Lactobacillus jensenii ).

The invention relates to an microcin C7 heptapeptide with an amino acid sequence of MATINAN. The heptapeptide has stronger biological characteristics of heat resistance, pepsin resistance, trypsin resistance and chymotrypsin resistance, high stability and strong bacteriostatic activity. The invention further provides a high-throughput screening method for obtaining a heat-resistant and protease-resistant strain. A lactobacillus johnsonii strain capable of producing the heptapeptide is screened out; the lactobacillus johnsonii strain is deposited in China General Microbiological Culture Collection Center on October 16th, 2019, and has the accession number CGMCC No.18695. The lactobacillus johnsonii strain has advantages of good stress resistance and high secretion amount and is low in cost.An important foundation is laid for industrial large-scale preparation of microcin C7 heptapeptide in the fields of animal husbandry feed, cosmetics, health care products and the like.

The invention discloses a composition capable of reducing halitosis and application thereof. The composition comprises composite probiotics, the composite probiotics comprise lactobacillus salivarius,bifidobacteria, lactobacillus rhamnosus, lactobacillus acidophilus, lactobacillus reuteri, streptococcus salivarius and lactobacillus johnsonii, wherein each gram of composition at least contains 2 billions of lactobacillus salivarius, 1 billions of bifidobacteria, 2 billions of lactobacillus johnsonii, 4 billions of lactobacillus rhamnosus, 2 billions of lactobacillus acidophilus, 2 billions oflactobacillus reuteri and 2 billions of streptococcus salivarius. The composition can also be widely applied in the preparation of drugs for treating halitosis, oral care products, cosmetic compositions, food or health-care products. Compared with the prior art, the composition disclosed by the solution of the invention can treat both intra-oral halitosis and extra-oral halitosis, and has the advantages of good therapeutic effect, broad application prospect and the like.

The invention discloses Lactobacillus johnsonii (Ljohn-1). Through the utilization of a dominant strain belonging to the vaginas of the women in our country and the utilization of applied technologiesthat the dominant strain can be applied to vaginal micro-ecological preparations, the cure rates of bacterial vaginitis can be increased, and the recurrence rates of the bacterial vaginitis can be reduced. The strain has been deposited with the China Center for Type Culture Collection in June, 2019 and assigned the accession number CCTCC No. M 2019426.

The invention provides a composite probiotic composition for inhibiting helicobacter pylori and an application of the composite probiotic composition. The composite probiotic composition comprises lactobacillus reuteri with a viable count of 1-2*10<10> CFU / g and lactobacillus johnsonii with a viable count of 3-4*10<10> CFU / g, and comprises the following other components in parts by weight: 80 to 160 parts of fructo-oligosaccharide, 400 to 600 parts of resistant dextrin, 200 to 400 parts of galactooligosaccharide, 8 to 12 parts of a liquorice root extract, 4 to 6 parts of a strawberry extract,and the balance of maltodextrin, wherein the total amount of the composition is 2000 parts. Probiotics contained in the composite probiotic composition are high in helicobacter pylori inhibiting capacity, the added components are beneficial to field planting of the probiotics and improvement of activity of the probiotics, and the stomach environment can be remarkably improved, so that helicobacterpylori can be effectively eradicated, and the composite probiotic composition has great application potential in the aspect of treating diseases caused by helicobacter pylori.

A food composition and pharmaceutical composition with strains of lactic acid bacteria for modulating blood glucose are provided, comprising an isolated lactic acid bacteria strain. The isolated lactic acid bacteria strain is at least one selected from a group including a GL-104 strain of Lactobacillus reuteri (CCTCC NO: M209138), an AP-32 strain of Lactobacillus salivarius subsp. salicinius (CCTCC NO: M2011127), a TYCA06 strain of Lactobacillus acidophilus (CGMCC No: 15210), and an MH-68 strain of Lactobacillus johnsonii (CCTCC NO: M2011128), or a combination thereof.

The invention discloses application of Lactobacillus johnsonii (preservation No.: CGMCC No.4926) to improvement of weaned pig production performance. After a proper amount of Lactobacillus johnsonii is added into feed, the effects of reducing weaned pig feed to gain (F:G) and improving weaned pig feed conversion rate are remarkable.

The invention discloses application of ((i) lactobacillus johnsonii( / i)) BS15 with collection number of CCTCC NO:M2013663 in preventing and treating non-alcoholic fatty liver (NAFLD) caused by high-fat food. The lactobacillus johnsonii BS15 remarkably lowers degeneration degree of fat mouse liver fat and apoptosis rate of liver cells.

The present invention relates to a preparation method of lipid lowering and weight losing compound probiotic plant enzyme powder. The preparation method is as follows: raw materials are refinedly selected; germinated oats are prepared; the raw materials of rhizoma polygonati, poria cocos, stir-fried hyacinth beans, germinated oats, Chinese yams, cortex cinnamomi, coix seeds, semen euryales, konjac and old radish roots are all soaked into hot water, the soaked materials are put into an extraction tank, water with an amount of 10 times of the amount of the raw materials is added, and extraction is performed at 100 DEG C or higher for 30-50 minutes; water with the amount of 8 times of the amount of filtered residues is added, and extraction is performed, and water with the amount of 6 times of the amount of the filtered residues is added, and extraction is performed, extracts are blended, the blended extract is filtered, the filtered extract is concentrated, filtered residues are dried, the dried filtered residues are crushed, the crushed filtered residues are sieved, filtering liquid is sprayed, the materials are mixed evenly, the mixture is placed in a fermentation bed, inoculation with saccharomyces cerevisiae, lactobacillus johnsonii and lactobacillus helveticus is conducted in an aseptic environment, and solid fermentation is conducted for 24-72 hours to obtain the lipid lowering and weight losing compound probiotic plant enzyme powder. The prepared plant enzyme powder can effectively reduce body weight, body fat, subcutaneous fat thickness, waist circumference and hip circumference, and lower the content of cholesterol and triglycerides. Examinations of electrocardiogram, etc. show no abnormities, endurance is not declined, and a dietary structure is not changed, indicating no harms on body health.

The invention belongs to the technical field of lactobacillus culture, and relates to a feeding lactobacillus johnsonii high-density fermentation medium and application thereof. By using the high-density fermentation medium and the lactobacillus johnsonii fermented by a fermentation method for the fermentation medium, the bacterial density is remarkably improved relative to common fermentation, the number of viable bacteria is not less than 1,010cfu / ml and is improved by over 80 times compared with the viable bacteria cultured by a common MRS culture medium, and high-density fermentation of the lactobacillus johnsonii is realized; and according to the fermentation medium and the application thereof, the separation cost of biomass is reduced, the production period is shortened, the production cost is reduced, and the production efficiency is improved.

The invention discloses an application of lactobacillus johnsonii CGMCC No.4926 in preparation of feed used for improving livestock immunity level. When the lactobacillus johnsonii is added into the feed in appropriate dosage, blood serum Newcastle disease antibody titer and IgA level of laying hens, laying chicks and broilers can be obviously improved.

The invention relates to the technical field of microorganisms, and particularly provides lactobacillus johnsonii and an application thereof. The preservation number of the lactobacillus johnsonii provided by the invention is CCTCC NO: M 2019976. The application of the lactobacillus johnsonii in preparation of products for regulating intestinal flora and the application of the lactobacillus johnsonii in preparation of antioxidants also belong to the protection range of the application. The strain provided by the invention is an excellent strain with potential probiotic properties, can be usedas a probiotic for antibacterial drugs, food additives and animal feed additives, and has high application prospects.

The invention discloses a strain of Lactobacillus johnsonii and its application in diarrhea of piglets, lambs and calves. The strain is isolated from healthy pig feces and has good in-vitro probioticpotential and safety. The Lactobacillus johnsonii of the invention and other strains are jointly applied to young animals with diarrhea, and the strain has obvious prevention and treatment effects ondiarrhea cases of different degrees.

The invention discloses broiler feed on the basis of lactobacillus johnsonii BS15 and a method for preparing the broiler feed. The broiler feed on the basis of the lactobacillus johnsonii BS15 contains 1*10<8>-1*10<9> cfu / kg of the lactobacillus johnsonii BS15. The service time of the boiler feed starts from one day after chicks are hatched to the broiler slaughtering time; the broiler feed and feed are mixed with each other and then are fed to broilers. The broiler feed and the method have the advantages that the problem of drug residues due to antibiotics can be solved, and gaps in the field after the antibiotics are forbidden can be filled; the intestinal tracts of the broilers can be regulated, the purpose of allowing the broilers to healthfully grow can be achieved, and growth promotion and disease prevention functions are integrated by a novel prevention and control process; the broiler feed is used for assisting in establishing healthful digestive systems of the broilers, and accordingly deterioration of the meat quality of the broilers can be prevented when sub-clinical necrotic enteritis occurs.

The invention discloses an application of Lactobacillus johnsonii CGMCC No. 4926 in improving production performance of laying chickens. The Lactobacillus johnsonii is added into feed in a dose of 1.0*104-108cfu / g, and accordingly feed conversion rate is improved and feed growth ratio of the laying chickens is lowered evidently.

The invention relates to a preparation method of compound nutritional powder of rice germs in a saline and alkaline land. The preparation method comprises the following steps of: choosing rice in the saline and alkaline land and mixing unknown minoriaoi, poria cocos, longan and oat with brown sugar water to obtain a culture liquid; inoculating compound probiotics of brewer's yeast, candida utilis and lactobacillus johnsonii to the culture liquid for fermental cultivation to obtain a domestication liquid; soaking the rice in the saline and alkaline land in the domestication liquid; putting the rice in a germinator to accelerate germination, and thorough rinsing the rice with water; drying the rice; and initially crushing the rice, adding oat powder and poria cocos powder, and fully stirring the mixture and performing superfine grinding to obtain the compound nutritional powder of rice germs in the saline and alkaline land. The rice in the saline and alkaline land is domesticated, cultured and germinated in the domestication liquid, and is good in palatability, solubleness and digestive absorption. The nutritional powder prepared by the preparation method provided by the invention is convenient to eat, unique in taste, rich in nutrition and long in expiration date; compared with universal 927 coarse rice powder, the lysine specific value is 136%, and the arginine specific value is 889%. Compared with trace elements in common rice meal, the contents of potassium, magnesium, zinc, selenium and manganese are high.

Provided herein are, inter alia, microbial compositions and methods of using the same. The microbial compositions provided include, inter alia, therapeutically effective amounts of Lactobacillus johnsonii, Faecalibacterium prausnitzii, Akkermansia muciniphila, Myxococcus xanthus and Pediococcus pentosaceus and are particularly useful for methods of treating and preventing inflammatory diseases.

The invention provides a composite probiotic preparation for targeting treatment on tumor and a preparation method of the composite probiotic preparation. By adopting the technical scheme, on the basis of physiological actions of various probiotics and synergic relationships of the probiotics, bifidobacterium adolescentis, lactobacillus crispatus, lactobacillus jensenii, lactobacillus vaginalis and lactobacillus johnsonii are adopted as preparation main bodies, on the basis, a compounding scheme is designed from angles of amount proportioning, numbers of viable organisms, and the like, and aneffective preparation method is provided. By adopting the preparation, targeting treatment on tumor can be achieved in an oral taking mode, and meanwhile the preparation can have synergetic effects with other anti-tumor medicines to promote anti-tumor effects of the preparation. The preparation is safe and non-toxic, free of conspicuous side effect, free of limits of tumor sizes and types, and good in quantitative treatment effect on various types of tumor.

The invention provides a brasenia schreberi enzyme and a preparation method thereof. The brasenia schreberi enzyme is prepared by fermenting the following raw materials and a compound microorganism: 42 weight percent to 63 weight percent of brasenia schreberi and 37 weight percent to 58 weight percent of fruits, wherein the fruits are of a mixture of oranges, apples, grapes, strawberries, pineapples, bananas, peaches, pears, almonds, muskmelons, watermelons, coconuts and blueberries; the compound microorganism is a mixed bacterium of lactobacillus delbrueckii subsp.lactis, lactobacillus gasseri, bifidobacterium adolescentis and bifidobacterium longum or a mixed bacterium of lactobacillus johnsonii, lactobacillus reuteri, bifidobacterium bifidum and bifidobacterium breve. The preparation method comprises the following steps: carrying out dual fermentation on the brasenia schreberi and the fruits through the compound microorganism and filtering and concentrating. By adopting the enzyme provided by the invention, the nutrient utilization rate of the brasenia schreberi is improved and the capability of human bodies is enhanced.