6 results about "Diarrheal diseases" patented technology

The application discloses an ELISA method for detecting porcine epidemic diarrhea virus antibody SIgA and belongs to the technical field of biology. The method comprises the steps of taking S1 protein as a coating antigen and detecting porcine epidemic diarrhea virus antibody SIgA in a sample to be detected, wherein the amino acid sequence of the S1 protein is a truncated sequence from the 19th to the 719th. The application preliminarily establishes an indirect ELISA method for detecting porcine epidemic diarrhea virus specific SIgA antibodies in serum, intestinal tissue and milk by optimizing conditions, and determines that the optimal antigen coating concentration and coating time are 0.25 mu g / ml and 4 DEG C overnight 12h-16h respectively, and the optimal detection conditions are determined. The method for detecting PEDV specific SIgA in serum, intestinal tissue and milk established in the research is simple and rapid, has good sensitivity and specificity, and provides a basis and a detection standard for evaluating the mucosal immune level of PEDV.

The application discloses a kind of pig multiple diarrhea virus detection method and application based on tNGS technology.The method realizes the accurate detection of pig epidemic diarrhea virus, porcine transmissible gastroenteritis virus and porcine delta coronavirus by tNGS technology.The primer set involved is used to amplify the S1 and M genes of PEDV, the N gene of TGEV and the M gene of PDCoV.The application also provides a kit comprising the primer set and a corresponding tNGS detection method.Clinical sample verification shows that the method is completely consistent with the qPCR detection results, has high sensitivity, strong specificity, can simultaneously identify mixed infection and other advantages, and has high detection throughput, controllable cost, is suitable for accurate diagnosis, epidemiological monitoring and prevention and control of diarrhea virus in pig farm, and has wide application prospect.

The invention discloses and relates to an antiviral RNA molecule capable of inhibiting porcine diarrhea virus and a design, preparation and toxicity detection method, the antiviral RNA molecule is PEDV-mhRNA, the antiviral RNA molecule is formed by connecting a plurality of siRNAs of S protein coding genes of targeted PEDV in series, an RNAi medicine capable of being used for preventing and treating PEDV is provided for domestic pig breeding, and the antiviral RNA molecule can be used for preventing and treating the PEDV. According to the present invention, the replication and the proliferation of the PEDV in the vero cell can be significantly inhibited, the method can be used for the prevention and the treatment of the PEDV, the RNA is adopted as the anti-virus factor, the characteristics of strong specificity, good anti-virus effect, no harm to the virus host and other organisms and the like are provided, the applicability is strong, the economic benefits of the breeding industry can be improved, and the promotion and application significance is provided.

The invention provides a synchronous detection primer for type II norovirus, group A rotavirus and adenovirus, which comprises specific primer pairs designed for conserved genes of three pathogens of type II norovirus, group A rotavirus and adenovirus, namely an ORF1 / ORF2 primer pair, a VP6 gene primer pair and a Hexon gene primer pair. The invention also provides a detection probe, which comprises an ORF1 / ORF2 capture probe, a VP6 capture probe and a Hexon capture probe. Meanwhile, the invention further provides application of the primer and the probe in preparation of infectious diarrhea related virus detection products, a specific detection kit and a specific detection method. According to the present invention, the multiple detection of three common diarrhea viruses can be achieved, the detection can be completed in a short time, the amplification product can be rapidly and accurately identified, the color development can be performed, the detection sensitivity and the specificity can be improved, the large-scale instrument is not required, and the cost is low.

This invention belongs to the field of virus detection technology, specifically relating to a monoclonal antibody against porcine delta coronavirus N protein and a latex immunochromatographic test strip containing this antibody. This invention uses prokaryotically expressed PDCoV N protein as an immunogen to immunize mice, and screens for specific monoclonal antibodies 2G3 and 9C4 through cell fusion and subcloning techniques. The test strip includes a PVC base plate, a sample pad, a conjugation pad, a nitrocellulose membrane (NC membrane), and an absorbent pad; a control line C and a detection line T are respectively set on the NC membrane. This test strip can specifically recognize PDCoV, with a detection limit as low as 10. 3.0 TCID 50 The virus has a concentration of 1000 μL / mL and does not cross-react with other common clinical diarrhea viruses. The results can be interpreted in just 15 minutes, making it fast, sensitive, and easy to operate.

The invention provides a primer probe combination for detecting diarrhea viruses through quadruple TaqMan qPCR and application of the primer probe combination, and belongs to the technical field of diarrhea virus detection. The primer probe combination for detecting the diarrhea virus through quadruple TaqMan qPCR provided by the invention comprises primer probes for detecting rotavirus, norovirus, human astrovirus and adenovirus respectively. The primer probe combination provided by the invention has the advantages of high sensitivity, stability, specificity and the like when being used for quadruple TaqMan fluorescent quantitative PCR detection.