20 results about "Polyproteins" patented technology

The present disclosure relates to methods, devices and systems for exposing microorganisms or compositions comprising functional proteins to an oscillating electric field substantially free of a magnetic component in order to improve stress responses, survival and metabolite or protein production. In embodiments, a controller drives one or more insulated emitters with a true AC signal or pulsed DC signal, in stepped, swept or shuffled patterns of an electric field oscillating at one or more frequencies in the range 210 Hz to 99 kHz. Exposure to the field provides surmounting energy that enhances protein breathing, catalysis, and fundamental functions of multiprotein complexes including transcription factors and ribosomes, thereby improving stress resistance of microorganisms, their viability during fermentation, drying, storage, transport, and / or increasing production of functional or therapeutic proteins, metabolites or microbial lysates. The technology is applicable to manufacture of probiotics, therapeutics, metabolites, food, alcohol, feed and bioenergy, cosmetics, nutraceuticals and biopharmaceuticals..

The invention discloses a cell strain capable of stably expressing a multi-protein tag as well as a construction method and application of the cell strain, and belongs to the technical field of biology. The cell strain is transfected by a lentiviral vector, a fusion expression vector containing P2A self-cleavage polypeptide and a plurality of protein tag coding sequences is integrated into a host cell, and a stable expression cell strain and a fusion protein expressing a plurality of protein tags including green fluorescent protein are obtained through antibiotic screening. The cell strain can be used for antibody specificity verification, protein interaction research and multi-label system standardization experiment platforms, and has the advantages of stable expression, simple operation, reliable result and the like.

Provided is a multi-protein degradation agent having an imide skeleton, which can degrade multiple proteins comprising c-Myc, GSPT1, CK1α and IKZF (1 / 2 / 3) proteins, and therefore can be used for treating diseases or disorders related thereto, such as cancer.

The present invention discloses the application of PHT-427 in the preparation of a drug for inhibiting the activity of the monkeypox core protease. By using high-throughput drug screening technology and through multiple screenings, a small molecule compound PHT-427 that can efficiently inhibit the core protease I7L was obtained. Through experiments, it was confirmed that the IC 50 value of PHT-427 for the monkeypox virus core protease I7L is 9 μM. In vitro enzyme activity experiments demonstrated that the compound PHT-427 can specifically bind to the active site of the monkeypox virus core protease, significantly inhibit the catalytic function of this protease, block the cleavage processing of viral polyproteins and the maturation and assembly of virus particles, and thereby exert an antiviral effect.

The present disclosure provides engineered Class 1 Type I CRISPR-Cas (Cascade) systems that comprise multi-protein effector complexes, nucleoprotein complexes comprising Type I CRISPR-Cas subunit proteins and nucleic acid guides, polynucleotides encoding Type I CRISPR-Cas subunit proteins, and guide polynucleotides. Also, disclosed are methods for making and using the engineered Class 1 Type I CRISPR-Cas systems of the present invention.

The invention relates to the technical field of early tumor screening and molecular diagnosis, in particular to a protein detection marker for screening early gastric cancer, and particularly relates to a mass spectrum-based multi-protein marker detection method. The protein composition shows excellent diagnostic performance in early gastric cancer detection, achieves an AUC value of 0.95 or above in a clinical queue, and has clinical application potential.

The present application provides a kind of based on PBA data's microorganism structure proteomics analysis method, by inputting the PBA original data of microorganism structure, extract the encoding nucleotide fragment containing specific information from the PBA original data, obtain intermediate transmission data;Then extract the protein expression data of the microorganism structure from the intermediate transmission data.From protein expression data, the total protein expression data of sample, the protein combination data of multiple protein co-expression, the subgroup data obtained after clustering analysis based on the protein expression data of the microorganism structure are analyzed to obtain machine learning.In the multi-level of protein expression, protein combination, subgroup, potential biomarker screening can be carried out.The analysis method described in the present application analyzes the total protein expression, protein combination form, microorganism structure subgroup and its protein fingerprint characteristics and quantification on the level of single microorganism structure, fills the blank of bioinformatics analysis in the field of microorganism structure proteomics detection.

The invention discloses a multiple fluorescent staining method for multiple protein targets, and relates to the technical field of multiple immunofluorescent staining. According to the method provided by the invention, the number of dyeable protein targets in the tissue slice can be greatly increased, multiple fluorescent staining steps can be simplified, and by adopting the method provided by the invention, imaging can be carried out after all the targets are directly stained on the basis of one tissue slice. The number of tissue slices required by dyeing imaging is reduced, dyeing of more than 6 protein targets can be completed by only one tissue slice, and errors caused by slice continuity differences are avoided. In addition, repeated elution and re-dyeing are not needed, so that the problems of antigen loss and weakening and the like caused by repeated elution are avoided.

The invention provides a method for separating multiple proteins in a milk raw material and application of milk protein.The method comprises the steps that the temperature of feed liquid is controlled to range from 50 DEG C to 55 DEG C, the influences of salt balance and beta-casein dissociation caused by the temperature are eliminated, and the stability of the milk protein in the feed liquid is kept; and then whey protein and casein are separated through chymosin treatment. Specifically, in the invention, chymosin treatment is carried out in a first reaction solution, so that whey in the first reaction solution can be separated out, which is caused by the fact that a k-CN micelle net-shaped structure is continuously rearranged in a curding process, and the whey distributed in the net-shaped structure is transferred. In the invention, by controlling the pH of a reaction system and the mass ratio of calcium to phosphorus in the chymosin treatment process, the content of whey separated out from the first reaction liquid can be remarkably increased, so that the yield of lactoprotein is further increased.

The invention discloses a multi-mode bar code based on a tubular DNA origami nanostructure and a preparation method of the multi-mode bar code, and belongs to the technical field of nanomedicine. Comprising a tubular DNA origami nanostructure and a plurality of fluorescently-labeled nucleic acid aptamers located on one side of the tubular DNA origami nanostructure, and the plurality of nucleic acid aptamers can be specifically combined with various cell surface target proteins and form an aptamer array; and biotin-modified oligonucleotides are arranged on the other side of the tubular DNA origami nanostructure, so that a biotin array is formed and is used for combining streptavidin-modified magnetic beads. By controlling stoichiometry, spatial orientation and modularization of the aptamer, efficient capture, multi-protein phenotype analysis and mild release of tumor cells with highly heterogeneous phenotypes are realized. The problem that in the prior art, capture and multiple analysis are difficult to integrate in rare cell analysis is solved, and a universal and intelligent nano platform is provided for cell sorting, proteomics analysis and molecular typing in precision medical treatment.

The invention relates to a protein interaction screening system based on a bacterial two-hybrid technology, which comprises a bait protein and prey protein co-expression plasmid pCDFduet1-lambda cI-RNAP, the sequence of which is as shown in SEQ ID No. 1; a reporter plasmid is pACYC184-cIamp; the sequence of the lac promoter-GFP is as shown in SEQ ID No. 2, and the lac promoter-GFP is as shown in SEQ ID No. 2; the positive control plasmid is a pCDFduet1-lambda cI-LG2-RNAP-Gal11p plasmid, and the sequence of the positive control plasmid is as shown in SEQ ID No. 3; the invention further relates to a high-throughput protein interaction screening method of mycobacterium tuberculosis toxin-antitoxin and application of the protein interaction screening system. The protein interaction screening system can realize high-throughput, high-sensitivity and semi-quantitative many-to-many protein interaction analysis by utilizing a flow cytometry sorting technology and three-generation sequencing analysis, effectively solves the problem of false negative caused by non-uniform conversion efficiency of a traditional double-plasmid system, remarkably improves the practicability of high-throughput screening, and has a good application prospect. The large-scale screening bottleneck is broken through, and wide application prospects are achieved.

The application discloses a kind of application of multi-protein marker and kit and system for early diagnosis of breast cancer, belong to biomarker technical field.The multi-protein marker includes PTN, IL12, CCL3, CASP-8 and ADGRG1.The application first five proteins PTN, IL12, CCL3, CASP-8 and ADGRG1 are used as combination marker for early diagnosis of breast cancer, and corresponding ELISA kit, diagnostic system and judging method are constructed, and the diagnostic method is high in sensitivity and specificity, can improve the early detection rate of breast cancer while guaranteeing specificity, and the early screening kit and diagnostic system constructed can also be applied to the screening of therapeutic drug for breast cancer, has wide application prospect, solves the problem of insufficient sensitivity, low specificity and poor stability of single index in the existing early diagnosis of breast cancer serology marker.

The invention discloses a 2-pyrrolidone tolerance target mining method based on proteomics and application of the 2-pyrrolidone tolerance target mining method, and belongs to the technical field of genetic engineering. According to the invention, based on a high-resolution proteome map, a system response network of toxicity stress is analyzed, and a multi-protein regulation node considering membrane steady state, redox steady state and pathway enzyme activity is more clearly locked; a protein combination is designed in a calculation-experiment closed loop mode, terminal tolerance improvement of yield and synchronous amplification of the yield can be achieved without sacrificing growth and substrate utilization rate, the influence of genes Ncg10464, Ncg11062, Ncg10453, Ncg10983, cg2886, cg0095, cg0344 and cg0507 on the tolerance of corynebacterium glutamicum to 2-pyrrolidone is excavated, the genes are used as a modification target, a gene engineering bacterium is constructed, and the gene engineering bacterium is applied to the field of gene engineering. The yield of 2-pyrrolidone can be obviously improved, and the highest yield can reach 20.69 g / L.

The application discloses a molecular recruitment co-localization system, comprising: a spatially targeted visualization module for recruiting target proteins to a specific site and generating a fluorescence co-localization signal in living cells based on the target proteins recruited to the specific site; a multi-protein interaction verification module for observing protein interactions in a multi-protein complex based on different pairs of proteins recruited to the specific site at a specified genetic locus; a protein binding capacity evaluation module for quantitatively analyzing the binding capacity between proteins based on the co-localization percentage and signal-to-noise ratio of the proteins; a protein phase separation capacity evaluation module for rapidly evaluating whether different proteins or protein domains have phase separation capacity based on the recruitment of the different proteins or protein domains to a specific location; and a dynamic observation and quantitative evaluation module for observing the dynamic changes of proteins in the phase separation process in real time by combining multi-channel fluorescence and quantum dot probes and providing preliminary quantitative data for the physical properties of the condensate.

This invention belongs to the field of biomedical technology, specifically relating to lactoferrin peptides and the application of lactoferrin in the preparation of inhibitors of 3C protease. The lactoferrin peptides of this invention contain the amino acid sequence shown in SEQ ID NO.1. The lactoferrin peptides and lactoferrin provided by this invention can specifically bind to and inhibit the activity of 3C protease, directly inhibiting viral replication by blocking its cleavage function of viral polyproteins. The mechanism is clear and has high affinity. Since 3C protease is highly conserved in the Picornaviridae family, it has broad-spectrum inhibitory effects against enteroviruses such as EV-A71 and CV-A9, and has a wide range of applications. Furthermore, it is derived from natural substances, has good biocompatibility and high safety, and can be made into various forms such as pharmaceuticals and antiviral daily necessities, with diverse application scenarios. This invention provides a new approach for developing antiviral drugs targeting 3C protease based on natural proteins, and has significant theoretical and applied value.

This invention relates to the fields of early tumor screening and molecular diagnostics, and particularly to protein biomarkers for screening early gastric cancer, especially a mass spectrometry-based method for detecting multiple protein biomarkers. The protein combinations described in this invention exhibit excellent diagnostic performance in early gastric cancer detection, achieving an AUC value of over 0.95 in a clinical cohort, demonstrating potential for clinical application.

The present disclosure provides engineered Class 1 Type I CRISPR-Cas (Cascade) systems, including a multiprotein effector complex, a nucleoprotein complex comprising a Type I CRISPR-Cas subunit protein and a nucleic acid guide, a polynucleotide encoding a Type I CRISPR-Cas subunit protein, and a guide polynucleotide. Also disclosed are methods of making and using the engineered Class 1 Type I CRISPR-Cas systems of the present disclosure.

The invention relates to a multicolor joint detection test strip for simultaneously detecting multiple proteins in transgenes as well as a preparation method and application of the multicolor joint detection test strip. According to the multi-color joint inspection test paper, a group of colored nano microspheres with different colors and compatible analysis conditions are screened as visual optical signal probes, BT-Cry1Ab / Ac, CP4-EPSPS and PAT / bar target antibodies are marked in a covalent bonding mode and are preset on a combination pad, different positions of a nitrocellulose membrane detection area are coated with transgenic target antibodies of different components, and the visual optical signal probes are used for detecting the target antibodies. And preparing the multicolor joint inspection test strip. According to the test paper, BT-Cry1Ab, BT-Cry1Ac, CP4EPSPS and PAT / bar protein can be detected at the same time, different colors are displayed at different positions of a nitrocellulose membrane detection area, the result is accurate, good specificity and stability and high sensitivity are achieved, the screening efficiency can be remarkably improved, and cross interference among all components is effectively reduced.

The invention relates to a polyprotein peptide composition and application of the polyprotein peptide composition in the aspect of hypoproteinemia, and belongs to the field of biological medicine. The polyprotein peptide composition comprises the following components in parts by weight: 40-60 parts of ovalbumin peptide; 15 to 30 parts of fish collagen peptide; 10 to 20 parts of sea cucumber oligopeptide; 5 to 15 parts of cordyceps militaris oligopeptide; and 5 to 15 parts of yeast beta-glucan. The ovalbumin peptide is used as a core protein source, the fish collagen peptide, the sea cucumber oligopeptide, the cordyceps militaris oligopeptide and the yeast beta-glucan are compounded, the components achieve a synergistic effect according to a specific weight ratio, the A / G protein inversion state of a patient suffering from the hypoproteinemia can be rapidly improved, wound granulation tissue hyperplasia and healing are promoted, physical strength is enhanced, fatigue is resisted, and the curative effect is good. The composition can be used for treating hypoproteinemia, enhancing lymphocyte proliferation and NK cell activity, reducing side effects of chemoradiotherapy, improving the curative effect of anti-tumor treatment and remarkably improving the prognosis of patients, and can be widely applied to nutrition intervention, postoperative rehabilitation and tumor chemoradiotherapy adjuvant therapy of hypoproteinemia patients.