394 results about "Infectious bursitis" patented technology

The invention aims at providing an I-colony fowl adenovirus 4 strain, which is preserved with preservation number of CCTCC No. V201541. The I-colony fowl adenovirus 4 YBAV-4 strain disclosed by the invention is excellent in specificity and immunogenicity; a specific precipitation line does not appear in specific positive serum chicken SPF chicken serum such as infected cell sap and egg drop syndrome resisting virus, chicken infectious bursal disease virus, Newcastle disease virus, chicken infectious laryngotracheitis virus, chicken Marek's disease virus, avian influenza and the like, while an obvious specific precipitation line appears in I-colony fowl adenovirus 4 specific serum. The strain disclosed by the invention, as a vaccine strain which is good in manufacturing effect, is capable of preventing chicken hydropericardium syndrome, and the strain is applicable to identification of virus serotype and investigation on epidemiology.

The present invention provides an infectious bursal disease virus (IBDV) subunit antigen-containing vaccine composition, which contains an immune amount of recombinant VP2 protein and an adjuvant. According to the present invention, with the subunit gene engineering vaccine composition, the infectious bursal disease can be effectively prevented and controlled; and with the purification technology adopted by the recombinant antigen protein, the endotoxin content in the vaccine composition is significantly reduced, the side reaction of the chicken individuals is significantly reduced, and the safety of the vaccine is substantially increased.

The invention discloses a recombinant low-virulent vaccine strain of chicken infectious bursal disease viruses (IBDV) and application thereof. In the invention, a major protective antigen gene VP2 of an epidemic superhigh virulent strain is cloned, the nucleotide of the gene VP2 is modified by mutation and then used for replacing a corresponding segment of a Gt genome of a low-virulent strain of the IBDV, so that the infectious clone of a recombinant genome of the IBDV is constructed, and the recombinant low-virulent vaccine strain is saved and identified by using an IBDV reverse genetic operation system. The microbial collection number of the vaccine strain is CGMCC No.3749. The recombinant low-virulent vaccine strain of the invention has high replicability, genetic stability and safety. The immune effect of the low-virulent vaccine strain of the invention is as good as that of the medium-virulent vaccine strain, but is superior to that of the low-virulent vaccine strain. The biological safety of the low-virulent vaccine strain of the invention is superior to that of the medium-virulent vaccine strain. As the vaccine strain, the recombinant low-virulent vaccine strain of the invention has the characteristics of high efficiency and low toxicity, is a good candidate vaccine strain and can be used for controlling chicken infectious bursal disease.

The invention relates to an infectious bursal disease virus (IBDV) HQ strain CGMCC NO.4935 and a preparation method of inactivated vaccines and combined vaccines for infectious bursal disease (IBD). The preparation method mainly comprises the following steps of: (1) carrying out chain amplification on cell seeds; (2) adding cell growth-promoting liquid into a sterilized bioreactor, and inoculating cells for preparing vaccines for suspension culture; (3) replacing maintenance liquid containing an IBDV strain when cells are of the maximum density, and continuing to culture; (4) collecting viruses in time, and measuring virus titer; and (5) inactivating virus liquid, and preparing inactivated vaccines and combined vaccines thereof for the IBD according to different proportions. The method provided by the invention increases the cell density, improves the virus titer, improves the vaccine titer, reduces the side reaction, reduces the labor intensity, lowers the production cost, improves the controllability of production processes, and ensures the uniformity and stability of product quality. The produced inactivated vaccines and combined vaccines thereof for the IBD have the advantagesof good safety and high immune efficiency and have a complete protective effect on the IBDV attack.

The invention provides an application of protocatechuic acid in the preparation of drugs for preventing and controlling livestock and poultry virus infectious diseases. The viruses include infectious bursal disease virus, avian influenza virus, infectious bronchitis virus and/or porcine transmissible gastroenteri tis virus. Meanwhile, the invention also relates to a protocatechuic acid preparation and a preparation method thereof.

The invention discloses a novel chicken infectious bursal disease virusVP 2c DNA (SEQ ID NO: 1), construction of its expression carrier, expressed recombined VP2 protein and the application of said recombined protein in preparing subunit genetic engineering vaccine against chicken infection bursal disease virus. The chicken infectious bursal disease virusVP 2c DNA can be highly expressed in yeast cell, and expressed recombined protein possesses biological activity and immunogenicity of the chicken infectious bursal disease virus natural protein . The expressed recombined protein can be produced into vaccine, and it is demonstrated through immunity chicken test that: the protein subunit vaccine can effectively induce body to generate spcial humoral immune response, make immunity chicken get 90% protection from deadly attack of highly pathogenic vv IBDV, and effectively prevent virus proliferation in the body.

The invention discloses two types of ScFv (Single Chain Variable Fragment) antibodies, encoding genes of the antibodies and applications of the antibodies for preparing the preparations for treating or preventing infectious bursal disease of chicken. Two single-chain antibodies (namely, the ScFv antibodies) are provided; and the ScFv antibodies can be specially bound with the protein 2 (VP2) (Virus Protein 2) in an IBDV (Infectious Bursal Disease Virus) structure and a plurality of IBDV strains to block the cytopathic effect (CPE) of the chicken embryo fibroblast by the IBDV so as to protect the young chicken infected with IBDV. The immune serum and egg yolk antibody are tedious in preparation, high in production cost, unstable in effect, difficult to control industrialized production quality, capable of causing horizontal disease spread and the like in a use process; the ScFv antibodies disclosed by the invention can overcome the above disadvantages and have the advantages of strong specificity, good treatment effect, controllable industrialized production quality, capability of preventing the horizontal disease spread caused by the egg yolk antibody and the like; and therefore, by virtue of the ScFv antibodies, a new situation is opened up in the IBDV prevention and treatment history, and even in the entire prevention and treatment history for animal virus diseases.

The invention relates to a bacterium for the production of genetically-engineered subunit oil-adjuvant vaccines against infectious bursal disease, i.e. Escherichia coli BL21/pET-28a-VP2, CCTCC No: M204038. The process for constructing the bacterium consists of inserting VP2 protein gene of the infectious bursal disease virus onto expression plasmid pET-28a to construct prokaryotic expression plasmid, then using the prokaryotic expression plasmid to transform the escherichia coli BL21, using lactose as inducer to induce Escherichia coli BL21/pET-28a-VP2 expression VP2 protein, purifying and charging oil-adjuvant to obtain the vaccines.

The invention discloses a method for extracting chicken egg yolk antibodies against infectious bursal disease, which comprises the following steps of: taking an immune egg yolk, breaking the immune egg yolk, and purifying by respectively adopting acetic acid-sodium acetate buffer solution, octylic acid solution and ammonium sulfate; and filtering by using a filter membrane to obtain the egg yolk antibodies. In the method for extracting the chicken egg yolk antibodies against the infectious bursal disease, the acetic acid-sodium acetate buffer solution, the octylic acid solution and the ammonium sulfate are respectively adopted for purification, and the filter membrane is adopted for filtration finally, so the loss of the antibodies and the damage to the antibodies are reduced, and the productivity of the egg yolk antibodies is improved; and the method has the advantages of simple operation, and convenient production, promotion and application.

The invention relates to a method for preparing a vaccine by breeding infectious bursal disease virus (IBDV) by a chicken embryo source cell line. The method mainly comprises the following steps of: 1) subculturing cells DF-1 for preparing vaccines; 2) breeding IBDV HQ cell seeds; 3) breeding virus liquid for preparing the vaccines; 4) concentrating and inactivating the virus liquid for preparingthe vaccine; 5) preparing other virus liquid of newcastle disease method, newcastle disease-infectious bronchitis virus method, and newcastle disease-infectious bronchitis virus-egg drop syndrome method combined vaccine and concentrating; and 6) proportioning inactivated combined vaccine, emulsifying and sub-packaging. The production process is simple, and stable and is easy to operate, eliminates biological potential safety hazard existing in the conventional vaccine production, and overcomes the defects that large-scale production of the vaccines is limited by supply of chicken embryos; cost and batch-to-batch variation are reduced; the virus titer and the quality of vaccine are improved; basis is laid for culturing virus liquid on large scale by a suspension culture technology in vaccine industry; and the produced IBDV inactivated vaccine and combined vaccine have high safety and immune efficacy, and have the complete immune protection effect on IBDV attack.

The invention relates to virus-like particle recombinant protein of a virus variation strain VP2 gene of an infectious bursal disease, belonging to the field of biologic pharmacy. The IBDV variation strain (AH1) VP2 gene is cloned, converted and transfected to obtain a recombinant baculovirus vBac-VP2; an infected Sf9 insect cell has specific fluorescence, and the antigen valence of the infected Sf9 insect cell is above 1.6*10<3>; the molecular weight of a recombinant VP2 protein is 53kDa, and the recombinant VP2 protein is in the state of virus-like particles; an indirect ELISA detection method established for an envelope antigen by the purified recombinant VP2 protein has good specificity and sensibility; an immune chicken can resist IBDV virulent attack, and the protection ratio achieves 100 percent. The novel virus-like particle recombinant VP2 protein prepared by the IBDV variation strain VP2 gene has high pertinence on the immune prevention of a current prevalent IBDV virulent strain and good practical value and popularization prospects.

The invention provides a GeXP quick detection kit and detection method for simultaneously identifying 8 chicken infectious immunosuppression disease pathogens. The kit is used on the basis of a GeXP system, and comprises 8 pairs of PCR (polymerase chain reaction) primers. The detection result indicates that the kit has the advantages of high specificity and high sensitivity and can be used for simultaneously identifying and detecting chicken Marek's disease virus, A, B and J subgroup avian leucovirus, avian reticuloendothelium hyperplasia virus, avian reovirus, chicken infectious anemia virus and infectious bursal disease virus.

The invention discloses an optimum condition for proliferating Guangxi epidemically representative strains of infectious bursal disease virus (IBDV) in chicken embryos. The optimum condition is an orthogonal design combination of seed virus inoculation route, inoculated embryo day age, inoculation density, embryo gathering time and gathering part when the virus candidate strain i.e. Guangxi epidemically representative strains 040124, BH11 and TSC-2(9), which are designed by biostatistics software, are proliferated in chicken embryos, and an optimum production parameter is explored to supply useful data to the commercial production of vaccines. The invention has the advantages of exploring the optimum condition and supplying useful data for the commercial production of the vaccines.

A preparation method of a purified vitelline freezing antibody for chickens infectious bursal disease is characterized in that: high purity antigens are adopted to vaccinate IBDV B87 to SPF chickens, then velvet urine, idiosome, velvet urine membrence are acquired, grinded, filtered and inactivated to antigens, and the steps are: producing immune eggs, collecting high immune egg, separating vitelline, diluting with distilled water, inactivating, extraction, decentering, inactivating, freezing and checking; the eggs is radiated by Co60 to kill viruses and bacterium, and finally froze by novel immuno-enhancer - Astragali Polysaccharoses and preserved under a temperature of 2-8 DEG C. Composite drugs for prevention and therapy are prepared by adopting the vitelline antibody for chickens infectious bursal disease as major components and cooperating with a novel immuno-enhancer, to decrease the jeopardy of the chicken breeding industry, and improve the living level of people and benefit mankind.

The invention relates to a preparation method of newcastle diseases, infectious bursal disease bigeminy high immunity-yolk-antibody lyophilized powder. The bigeminy yolk-antibody lyophilized powder is prepared by preparing vaccines by preparing strains with strong immunity, preparing high immunity eggs, conducting disinfection, acidizing, octanoic acid treatment, refined exaction purification treatment, preparation and freeze-drying. The newcastle diseases, infectious bursal disease bigeminy high immunity-yolk-antibody lyophilized powder prepared by using the method is a preparation capable of simultaneously preparing the newcastle diseases and chicken infectious bursal diseases, clinically reduces manipulation strength and reduces raise cost. A newcastle diseases, infectious bursal disease bigeminy high immunity-yolk-antibody lyophilized preparation is convenient to transport and store, is long in saving time and can keep high valence of antibody.

The invention provides a concentrated freeze-dried yolk antibody composite preparation for infectious bursal disease, which contains the following components in part by weight: 10.0 to 99.5 parts of infectious bursal disease virus resistant yolk antibody and 0.5 to 20.0 parts of Chinese medicinal polysaccharide, wherein the Chinese medicinal polysaccharide is extracted from one or a composition of more than two of the following Chinese medicines in part by weight: 10 to 50 parts of Chinese angelica, 50 to 100 parts of szechwon tangshen root, 50 to 80 parts of membranous milkvetch root and 30 to 50 parts of Indian buead. The preparation is a freeze-dried preparation prepared by combining the components such as the infectious bursal disease virus resistant yolk antibody and the polysaccharide under the guidance of immunity engineering of Chinese medical science, is clinically used for the infectious bursal disease, has the effect of treating infectious bursal disease virus infection, and excites the immunity and disease resistance of organisms; and simultaneously, the polysaccharide component in the preparation has powerful protection effect on the yolk antibody.

The invention provides a neglect-proof drinking immunization tablet for infectious bursal disease (IBD), which comprises an antigen for IBD, a heat-resistant protective agent and an effervescent material, wherein the antigen for IBD and the heat-resistant protective agent are mixed to prepare a heat-resistant antigen freeze-dried particle, and then, the particle and the effervescent material are evenly mixed and pressed to obtain the neglect-proof drinking immunization tablet. Because a color indicator is added into the effervescent material, by checking whether chicken beak tips are dyed or not, the chicken which does not drink water can be easily found, and the chicken can drink water, thereby effectively avoiding the occurrence of an immunization neglect phenomenon; and a metal chelator, an immune potentiator and a heat-resistant protective agent are added, and a tablet is prepared, thus the vaccine is convenient to transport and store, and the storage time exceeds 24 months. When in use, the vaccine tablet provided by the invention only needs to be put into a proper amount of water for chicken flocks to drink to enable the chicken flocks to be immunized, thereby realizing the effective immunization of IBD of the chicken flocks, ensuring the immunization uniformity, preventing the immunization neglect, and enhancing the vaccine immunization effect. The tablet provided by the invention is most suitable for medium-and-large-sized chicken farms.

The invention provides an anti-chicken infectious bursal disease (IBD) recombinant protein subunit vaccine. The vaccine is a fusion protein having high immunogenicity of Salmonella typhimurium flagellin and an infectious bursal disease virus (VP2). The above flagellin + VP2 fusion protein is obtained through the expression of a recombinant baculovirus containing a flagellin + VP2 gene by utilizing a Bac-to-Bac baculovirus expression system. The recombinant baculovirus obtained through the system has a short period, and the expressed flagellin + VP2 fusion protein has high immune protection force.

The invention discloses a heat resistant lyophilized protectant for infectious bursal disease living vaccine and a preparation method thereof. The heat resistant lyophilized protectant comprises the following compositions in percentage by mass volume: 2 to 7 percent of lactose, 0.2 to 0.6 percent of milk extract, 0.2 to 0.8 percent of lactoalbumin hydrolysate, and the balance being water for injection. The protectant and the method overcome the defects of complicated compositions, high cost and the like generally existing in the prior heat resistant lyophilized protectant; and the heat resistant lyophilized protectant has the advantages of good heat resistance, effective protection of the living vaccine, simple formulation compositions and formulation method, low production cost, mass production and the like, and can ensure that the infectious bursal disease living vaccine can be stored for more than 24 months at a temperature of between 2 and 8 DEG C, and for 1 month at 37 DEG C. The protectant and the method solve the problems of low-temperature refrigerating transportation, inconvenient storage, high cost and the like required by the infectious bursal disease living vaccine.

The invention relates to a compound preparation for treating the virosis of birds and the preparation method of the compound preparation. The compound preparation is prepared by mixing raw materials by weight portions, 20 to 50 parts of milk veteh and 30 to 50 parts of grifola frondosa. Preparation procedures are as follow: The raw materials are extracted by soaking, decocted by heating and precipitated by filtering; therefore the Chinese herbal medicine is prepared. The compound preparation has the beneficial effect as follows: (1) the new preparation that can control the virosis of the birds and strengthen the disease resistance of organism is created for clinical treatment; (2) the compound mode is reasonable, the antiviral capability is strong and all the selected medicine is the effective medicine for treating the virosis of birds; (3) the compound preparation has the advantages of advanced technology, stable preparation and long storage life; (4) the compound preparation has the advantages of being safe to use, definite healing effect and controllable quality; (5) the compound preparation has the advantages of wide source of the raw materials, being avirulent, no side effect and being uneasy to cause drugfastness, so as to meet the standard of international hygienic examination. The compound preparation is especially applicable for treating infectious bursal disease, atypical newcastle disease and infective bronchitis, and the treating effect is remarkable.

The invention relates to a method for producing a quadruple inactivated vaccine for newcastle disease, infectious bronchitis, avian influenza (H9 subtype) and infectious bursal disease. In the method, the inactivated vaccine is prepared by adopting a newcastle disease virus La Sota strain, an infectious bronchitis virus M41 strain, an avian influenza virus (H9 subtype) YBF003 strain, and an Escherichia coli genetic engineering bacteria E. coil BL21/pET28a-VP2 strain which expresses an infectious bursal disease virus VP2 protein serving as production bacteria toxic species, a super concentration process and a high-quality adjuvant. After immunizing animals, antibodies are produced rapidly; the potency is high; the protection period is long; and the outbreak and the spreading of epidemic diseases are reduced.

The invention discloses a scFv antibody, an encoding gene thereof and application thereof to preparation of preparations for treating or preventing an infectious bursal disease. The scFv antibody is formed by a heavy chain variable region, a light chain variable region and a connecting region between the heavy chain variable region and the light chain variable region. The heavy chain variable region is (a) or (b), wherein (a) refers to protein which is formed by amino acid residues at the first site to the 124th site of the sequence 1, and (b) refers to protein which is subject to substitution and/or depletion and/or addition of the amino acid residues of (a) and has the same activity; and the light chain variable region is (c) or (d), wherein (c) refers to protein which is formed by amino acid residues at the 140th site to the 244th site of the sequence 1, and (d) refers to protein which is subject to substitution and/or depletion and/or addition of the amino acid residues of (c) and has the same activity. The scFv antibody provided by the invention has the advantages of high specificity, good curative effect and the like and creates a new situation in the prevention and treatment history of IBDV (Infectious Bursal Disease Virus), and even in the prevention and treatment history of the entire animal virus diseases.

The invention discloses a VP2 gene restructuring Newcastle disease LaSota weak virus strain rLasota-VP2 of Infectious bursal disease virus, IBDV and appliance in the Newcastal disease virus,NDV and IBDV vaccine.

The invention discloses a visual protein chip for detecting serum antibody of new-castle disease virus of chickens, infectious bronchitis virus of chickens, avian influenza virus and infectious bursal disease virus of chickens , which is prepared by the following steps: purifying and diluting whole proteins of the four virus respectively; pointing samples of the positive control serum, the negative control serum and the four virus proteins onto a chip carrier respectively; drying, fixing, sealing and washing the samples to obtain the visual protein chip. The visual protein chip uses the purified whole proteins as capturing antigens to detect the virus-specific antibodies in chicken serum so as to simplify the preparation technology and reduce the production cost, and the visual protein chip has better specificity but no cross, has high reliability of results and has the advantages of quickness, simplicity and convenience, high sensitivity, good specificity and the like. When the serum is diluted by 6,400 times, the visual protein chip still can detect the antibodies, the sensitivity is 400 times of that of the prior AGP detection method. According to the detection to serum samples in-place, the detection rate of the visual protein chip is higher than the proir AGP method remarkably.

The invention provides a traditional Chinese medicine microbial ecological agent for preventing and treating infectious bursal disease of chicken and a preparation method thereof. The preparation method comprises the following steps: adding water to radix isatidis, honeysuckle, forsythia, folium isatidis, humulus scandens, radix scutellariae, dangshen, ageratum, plantain, astragalus membranaceus, angelica sinensis and liquorice, boiling by using high heat, preserving heat for 30-60 minutes by using slow fire, cooling, filtering, collecting filtrate, adding white sugar, boiling, cooling to a normal temperature, then inoculating lactobacillus, zygosaccharomyces bailii and acetobacter xylinum, and fermenting for 3-7 days so as to obtain the microbial ecological agent. After the microbial ecological agent is diluted proportionally, the diluted microbial ecological agent is directly drunk by chicken. The traditional Chinese medicine microbial ecological agent provided by the invention has the effects of resisting viruses, inducing interferon, increasing the quantity of effective microbial communities in intestinal tracts, boosting immunity and improving disease resistance of chickens without toxic or side effects and the like; the preparation method is simple, convenient to use and high in viable bacteria content and has no residues. The traditional Chinese medicine microbial ecological agent provided by the invention has a certain curative effect on infectious bronchitis and henpox and relatively good market prospect.

The invention discloses an infectious bursal disease virus strain A11 and application thereof, and belongs to the field of the microbial technology. Infectious bursal disease viruses are separated from infectious bursal disease vaccine immunity failure chicken flocks appearing in recent years, biological characteristic analysis is carried out on obtained virus isolate strains, and the infectious bursal disease virus strain A11 with good biological characteristics is screened out from the obtained virus isolate strains and is a currently-popular infectious bursal disease virus with super-strong virulence. The virulence of the virus is attenuated with a chicken embryo and cell continuous passage method to culture the low-virulence strain A11, the low-virulence strain A11 has super-strong reproductivity in cells, the virus yield in cells reaches up to 10<11> TCID50/0.1 ml, immune protection resisting infectious bursal disease viruses can be rapidly generated by immunizing chicks with the strain A11, immune protection can be achieved after 1 week, and the strain A11 has huge application value in developing new specific infectious bursal disease vaccines.