33 results about "Infectious bursitis" patented technology

The invention relates to the technical field of biological medicines, and particularly discloses a preparation method and application of intestinal tract targeted DNA (deoxyribonucleic acid) nano origame.The preparation method is technically characterized by comprising the following steps: S1, synthesizing planar DNA nano origametes (DONs); s2, assembling the DONs and the siRNA (small interfering ribonucleic acid); and S3, synthesizing the tubular DNA nano origami loaded with siRNA: uniformly mixing the purified DONs-siRNA origami with 10 times of excessive APE1 chain and cross-linked chain, annealing from 45 DEG C to 25 DEG C, and synthesizing the tubular DNA nano origami. According to the method disclosed by the invention, a nano delivery carrier which has intestinal targeting, is accurately loaded and can release siRNA as required is developed by utilizing a DNA (Deoxyribonucleic Acid) nano technology, so that efficient treatment of IBD (Infectious Bursal Disease) by siRNA is realized.

The invention discloses an antigen fusion protein and application thereof in preparation of a live vaccine for preventing infectious bursal disease. Specifically disclosed are antigen fusion proteins comprising a VP2 protein, a trimer tag, and a C3d protein. The invention also discloses recombinant Eimeria heap constructed by using the antigen fusion protein and application of the recombinant Eimeria heap in preparation of IBDV live vaccines. The oocyst of the recombinant Eimeria heap can effectively induce humoral immune response aiming at IBDV as a vaccine active ingredient, the level of an induced antibody is obviously higher than that of an insect strain expressed by a single VP2 antigen, viruses can be more effectively neutralized, the immune protection efficacy is improved, the safety is high, and the oocyst has the potential of serving as a live vector genetic engineering vaccine. The IBDV live vaccine can be orally taken through drinking water or feed, vaccination is simple, large-scale immunization of chicken flocks can be achieved, and the IBDV live vaccine is economical and efficient and has a wide prospect of being applied to prevention and control of the IBDV of the chicken flocks.

The application provides application of a formulation of Usnea, Usnic acid or Usnic acid salt in preparation of a product for preventing and treating infectious bursal disease of poultry or complications caused by infection. The application can achieve an outstanding effect of resisting chicken infectious bursal disease by adding the formulation of Usnea or Usnic acid and its salt in feed or water. Meanwhile, the formulation of Usnea or Usnic acid and its salt is low in cost and simple in application as a means of resisting chicken infectious bursal disease, and is beneficial to large-scale promotion.

This invention relates to a traditional Chinese medicine preparation for treating infectious bursal disease (IBD), its preparation method, and its application. The preparation comprises the following components: Astragalus membranaceus 45-50 parts, Rehmannia glutinosa 30-45 parts, Isatis indigotica 25-30 parts, Isatis tinctoria 25-30 parts, Gypsum fibrosum 10-45 parts, Lonicera japonica 10-20 parts, Forsythia suspensa 10-30 parts, Taraxacum mongolicum 10-20 parts, Atractylodes macrocephala 15-20 parts, Paeonia lactiflora 15-30 parts, Lilium brownii 15 parts, Anemarrhena asphodeloides 15 parts, and Glycyrrhiza uralensis 10-30 parts. The preparation is achieved through extraction and concentration processes and is effective in treating IBD in chickens caused by both traditional infectious bursal disease virus (IBDV) and novel variant infectious bursal disease virus (nvvIBDV). This medicine significantly improves the cure rate against both traditional and variant strains, showing a particularly significant advantage in treating variant strains.

The invention belongs to the technical field of traditional Chinese medicine, and provides a traditional Chinese medicine composition for treating inflammatory bowel disease and a preparation method thereof, the traditional Chinese medicine composition comprises the following raw materials by weight: 0.5-5 parts of ginseng, 4-20 parts of Radix Astragali, 3-20 parts of Rhizoma Polygonati, 0.5-6 parts of licorice, 0.5-6 parts of cassia twig, 0.5-5 parts of processed monkshood, and 1-9 parts of ginger. The pharmaceutical composition disclosed by the invention consists of ginseng, astragalus membranaceus, rhizoma polygonati, liquorice, cassia twig, radix aconiti carmichaeli praeparata and ginger, is used for treating and conditioning IBD (Infectious Bursal Disease) with unknown etiology, and has a remarkable effect.

The application belongs to the technical field of biological medicine, and discloses a method for preparing an avian infectious bursal disease VP2 nanoparticle oral vaccine by using Kluyveromyces marxianus and application thereof. The applicant assembles and prepares a double replicon expression vector suitable for Kluyveromyces marxianus through screening, and the vector achieves the effect of high conversion rate and high expression efficiency in Kluyveromyces marxianus. The double replicon expression vector pGKD32 is shown in SEQ ID NO. 2. The applicant successfully expresses the avian infectious bursal disease VP2 in the form of nanoparticles by using the Kluyveromyces marxianus expression system provided in the application, and provides a new idea for the research and development of the avian infectious bursal disease virus oral immunization strategy.

The invention discloses colloidal gold immunochromatography test paper for rapidly detecting pigeon I-type paramyxovirus and a preparation method of the colloidal gold immunochromatography test paper. Viral diseases of pigeons, especially pigeon plague virus and pigeon adenovirus, are rapidly detected on the basis of a newly developed test strip, and the operation is simple and convenient; according to the design of antigens and antibodies of the pigeon plague virus and the pigeon adenovirus, rapid detection of the pigeon plague virus and the pigeon adenovirus can be conveniently carried out, and the obtained test strip is high in specificity and high in sensitivity. The pigeon I-type paramyxovirus detection kit has the advantages that the pigeon I-type paramyxovirus detection kit does not have cross reaction with chicken infectious bronchitis, chicken egg drop syndrome, chicken Marek's disease, avian influenza, fowl adenovirus, infectious bursal disease, escherichia coli, salmonella and other samples, but can have cross reaction with Newcastle disease, so that the pigeon I-type paramyxovirus detection kit can be used for diagnosing pigeon I-type paramyxovirus and Newcastle disease.

The invention discloses an IBDV (Infectious Bursal Disease Virus) mutant based on VP1 protein T425 and R426 synergistic modification and application of the IBDV mutant. The invention identifies that threonine at the 425th site on the VP1 protein is a new phosphorylation site for the first time, and reveals that threonine at the 425th site and adjacent arginine methylation modification at the 426th site have a synergistic positive feedback regulation relationship. Based on the mechanism, a T425 and R426 double-site modification deleted virus mutant is successfully constructed, and the mutant shows a phenotype with severely weakened replication ability. In addition, the invention also provides an antibody capable of specifically recognizing pT425, an application of a GSK-3 kinase inhibitor in inhibition of virus replication, and a related screening and detection method. The invention provides a new target and strategy for developing novel IBDV attenuated vaccines, antiviral drugs and diagnostic reagents.

The invention belongs to the technical field of biological medicines, and particularly relates to application of mesenchymal stem cells combined with mesalazine in preparation of an anti-IBD (infectious bursal disease) medicine. The mesenchymal stem cells are mesenchymal stem cells from human umbilical cord; the human umbilical cord mesenchymal stem cells highly express CD73, CD90 and CD65, and low express CD34, CD45 and HLA-DR (Human Leukocyte Antigen-DR). The research finds that the hUC-MSCs has a remarkable treatment effect on a chronic IBD mouse model when being singly used or combined with 5-ASA for treatment, the hUC-MSCs and drug combination can restore the intestinal microecological balance by adjusting the composition of intestinal flora so as to improve the symptoms of IBD, and the hUC-MSCs and the drug combination have a synergistic effect when being combined with 5-ASA for treatment. The invention provides a new thought and experimental basis for clinical treatment of IBD.

The invention discloses a chicken infectious bursal disease virus VP2 protein, a VP2 protein virus-like particle vaccine and a preparation method thereof. The method comprises the following steps: firstly, optimally designing an IBDV VP2 protein; secondly, a pCold II-SUMO prokaryotic expression vector is obtained by adding SUMO protein on the basis of an escherichia coli cold shock expression vector pCold II, the prokaryotic soluble expression efficiency of the VP2 protein is improved, and the concentration of the purified VP2 protein with the SUMO label can reach 1.00 mg / mL; after the SUMO label is removed, the VP2 protein can be 100% assembled into VLPs in a specific solution, and after emulsification-demulsification, the VP2 protein still keeps a complete VLPs structure instead of a VP2 monomer, the stability is good, and experimental results show that the VLPs vaccine can 100% protect SPF chicken from IBDV virulent virus attack. Besides, the preparation method of the VP2 protein is simple, molecular sieve chromatography purification with high cost is avoided, complex processes such as ultracentrifugation are not needed, the recovery rate of two-step affinity purification is close to 60%, the purity of the purified VP2 protein is about 80%, and industrial-scale preparation of IBDV structural protein and IBDV VLPs vaccines becomes possible.

A vaccine includes a recombinant herpesvirus of turkeys (HVT) vector. The HVT vector has a heterologous polynucleotide coding for and expressing an Infectious Bursal Disease Virus (IBDV) viral protein 2 (VP2) antigen and a heterologous polynucleotide coding for and expressing an Infectious Laryngotracheitis Virus (ILTV) glycoprotein D (gD) antigen. The two heterologous polynucleotides are inserted into one locus in a non-essential region of the HVT genome selected from intergenic region 1 locus, intergenic region 2 locus, intergenic region 3 locus, UL43 locus, US10 locus, US2 locus, and SORF3 / US2 locus. The two heterologous polynucleotides are linked by internal ribosome entry site (IRES). The expression of the two heterologous polynucleotides is driven by a cytomegalovirus (CMV) immediate early (IE) promoter.

The invention discloses a vaccine composition, an immunogen of the vaccine composition is a fusion protein containing a VP2 protein of a new isolate of chicken infectious bursal disease and an auxiliary protein, and the immunogen can be independently assembled into subvirus particles. The vaccine composition has good safety to chickens, the titer of an antibody generated by immunizing the chickens is high, and the challenge protection effect of the immunized chickens is good. The vaccine has an application prospect.

The present invention refers in general to the field of veterinary medicine and especially to the development of vectored vaccines using the Gallid alphaherpesvirus 3 (GaHV-3) or Marek's disease serotype 2 (MDV-2) vector, which contains protective antigens against different avian pathogens such as Newcastle disease virus (NDV), avian infectious laryngotracheitis virus (ILTV), infectious bursal disease virus (IBDV) or Gumboro disease virus, avian influenza virus (AIV), infectious bronchitis virus (IBV).

The invention belongs to the technical field of poultry vaccines, and particularly relates to a chicken new tributary method adeno-quintuplet vaccine and a preparation method thereof.The chicken new tributary method adeno-quintuplet vaccine comprises chicken Newcastle disease venom, infectious bronchitis venom, avian influenza venom, infectious bursal disease virus liquid and fowl adenovirus venom, and the preparation method of the Newcastle disease venom comprises the steps that firstly, frozen MDCK suspension cells are provided; the frozen MDCK suspension cells are recovered at the temperature of 40-45 DEG C; 2, transferring the resuscitated MDCK suspension cells to a serum-free culture medium; according to the invention, the inactivated vaccine is prepared from the Newcastle disease venom, the infectious bronchitis venom, the avian influenza venom, the infectious bursal disease virus liquid and the fowl adenovirus venom by adopting a superstrong concentration process and a high-quality adjuvant, so that after animals are immunized, antibodies are generated quickly, the titer is high, the protection period is long, and the occurrence and spreading of epidemic diseases are prevented; and people can conveniently put multi-combined vaccines to cope with continuously changing epidemic disease prevention and control situations.

The application provides a method for expressing chicken infectious bursal disease virus VP2 protein and application thereof, and belongs to the technical field of genetic engineering and veterinary biological products. The application constructs a recombinant eukaryotic expression vector containing an optimized IBDV VP2 gene sequence and transfects the recombinant eukaryotic expression vector into LMH suspension cells, and the high-efficiency expression is carried out under a serum-free suspension culture condition. The chicken infectious bursal disease virus subunit vaccine prepared by using the expression method has high safety and good immunization effect (the attack protection rate can reach 100%), and is suitable for large-scale industrial production.

The invention discloses a dual fluorescent quantitative PCR (Polymerase Chain Reaction) detection method for an infectious bursal disease virus strain, and belongs to the technical field of strain detection. The invention establishes a dual fluorescent quantitative PCR (Polymerase Chain Reaction) detection method capable of identifying the Lx strain and the Lx Mut422 strain of the infectious bursal disease through one-time PCR. Comprising the following steps: designing a specific primer and a fluorescence labeling probe according to a specific gene sequence of the infectious bursal disease virus; preparing a PCR (Polymerase Chain Reaction) amplification reaction system, and carrying out fluorescent quantitative PCR amplification by taking the RNA of the sample to be detected as a template; and detecting a fluorescence signal of an amplification product, and specifically detecting the Lx strain and the Lx Mut422 strain of the infectious bursal disease. According to the detection method provided by the invention, high sensitivity of a PCR technology and accuracy of a fluorescent quantitative technology are combined, and rapid and accurate identification of the Lx strain and the Lx Mut422 strain of the infectious bursal disease can be realized.

The invention belongs to the technical field of biology, and discloses an infectious bursal disease virus suitable for full suspension culture and application of the infectious bursal disease virus. The invention discloses a bursal disease virus strain, the bursal disease virus strain is named as IBDV-DHN and preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: V202572. The bursal disease virus IBDV-DHN strain is obtained through separation and screening, and the virus strain can adapt to full suspension culture and can be used for large-scale production of bursal disease vaccines. Meanwhile, the strain can adapt to reverse genetic modification, and the pathogenicity, the interspecific transmission capacity and the virus variation mechanism of the IBDV are explored from the molecular level. The virus strain can be used as a strain skeleton to construct vaccine candidate strains with different specificity, provides more specific immune protection for immunizing poultry, and has a wide application prospect.

The invention belongs to the technical field of biology, and particularly relates to a high-expression IBDV (infectious bursal disease virus) VP2 virus-like particle vaccine and a preparation method thereof, and the high-expression IBDV VP2 virus-like particle vaccine is prepared from chicken infectious bursal disease virus VP2 nucleotide sequences SEQ ID NO.1-3. According to the invention, codon optimization is carried out on five amino acids in a VP2 sequence of a chicken infectious bursal disease (IBDV) strain according to the preference of a yeast codon to form three different sequences, and the three different sequences are matched with different signal peptides to screen a codon-optimized sequence with the highest expression quantity, so that the protein expression quantity reaches more than 1g / L, and the immune cost is reduced. After the yeast fermentation liquor is simply purified, the purity of 70% or above can be achieved, the agar diffusion titer can reach 1: 128 after animals are immunized, and after chickens are immunized, the target protein can well protect BC6-85 strain viruses.

The invention discloses a hybridoma cell with a microbial preservation number of CCTCC (China Center For Type Culture Collection) NO: C2024404, and further discloses a monoclonal antibody secreted by the hybridoma cell. The monoclonal antibody can recognize the chicken infectious bursal disease virus VP2 protein with the amino acid sequence shown as SEQ ID NO.1 or SEQ ID NO.4, but cannot recognize the chicken infectious bursal disease virus VP2 protein with the amino acid sequence shown as SEQ ID NO.2 or SEQ ID NO.3, and has the application value of chicken infectious bursal disease virus strain specific recognition.

The present application relates to the field of microbiological technology, and particularly relates to a chicken infectious bursal disease virus, egg yolk antibody and a preparation method thereof. The chicken infectious bursal disease virus has a preservation number of CGMCC No. 46653. The strain is an independent new strain through VP2 gene sequencing and genetic evolution analysis. The present application also provides a method for preparing the egg yolk antibody by using the strain: immunizing hens by using an increasing dose immunization procedure to obtain immune hen eggs; disinfecting the immune hen eggs and separating egg yolk; extracting, settling and purifying the egg yolk to obtain the egg yolk antibody. In the method, a double disinfection system combining quaternary ammonium salt disinfectant and aldehyde disinfectant is used for disinfection; and a settling agent containing a high molecular flocculating agent and a protein stabilizer is added in the settling process. The titer of the egg yolk antibody prepared by the method can reach 1:256 or above, and the egg yolk antibody has good prevention and treatment effects on the chicken infectious bursal disease virus.

The invention provides a recombinant coccidiosis vector for expressing VP2 protein and a fluorescent label and a detection method of the recombinant coccidiosis vector, and belongs to the technical field of genetic engineering. The invention also provides sgRNA (small guide ribonucleic acid) targeting the ETH00009555 gene, and the sgRNA can be used for carrying out accurate gene fixed-point insertion; a gene editing system is established by sgRNA and homologous recombinant fragments, so that target fragments such as chicken infectious bursal disease virus VP2 protein and fluorescent label protein can be accurately subjected to homologous recombination on the ETH00009555 gene, and a recombinant coccidiosis vector for expressing the VP2 protein and a fluorescent label is constructed. The recombinant coccidium vector DNA is subjected to PCR (Polymerase Chain Reaction) detection and sequencing, and the result shows that the VP2 gene and the fluorescent tag gene are successfully recombined with the ETH00009555 gene; and the recombinant coccidiosis vector has an obvious fluorescence signal under a fluorescence microscope, which indicates that the recombinant coccidiosis vector successfully expresses the VP2 protein and the fluorescence tag protein.

The invention relates to the technical field of veterinary medicine and animal models, in particular to a modeling method for inducing broiler chicken immunosuppression and secondary bacterial infection. The method comprises the following steps: (a) starting to continuously feed broiler chickens with daily ration containing mycotoxin at the age of 6-10 days until molding is finished; (b) inoculating infectious bursal disease virus on the broiler chicken at the age of 12-16 days; and (c) inoculating avian pathogenic escherichia coli after inoculating the infectious bursal disease virus for a period of time. According to the modeling method, the clinical pathogenesis process is highly simulated, the mechanism fits the reality, the model success rate is high and stable, standardized operation is achieved, repeatability is good, simplicity, convenience and safety are achieved, and the biological safety risk of virulent strains is avoided; the application range is wide, and an excellent platform is provided for research and development; the method is low in cost and suitable for scientific research and large-scale drug screening popularization.

The present invention discloses novel recombinant multivalent non-pathogenic Marek's Disease virus constructs that encode and express both Infectious Laryngotracheitis Virus protein antigens and an Infectious Bursal Disease virus protein antigen, and methods of their use in poultry vaccines.

The present invention relates to the veterinary field in general and specifically to the development of vectored vaccines using the Gallid alphaherpesvirus 3 (GaHV-3) or Marek's disease serotype 2 (MDV-2) vector, which contains protective antigens against different avian pathogens such as Newcastle disease virus (NDV), infectious laryngotracheitis virus (ILTV), infectious bursal disease virus (IBDV) or Gumboro disease, avian influenza virus (AIV), and infectious bronchitis virus (IBV).

The invention belongs to the technical field of chicken disease prevention, and particularly relates to a quadruple inactivated vaccine for preventing chicken diseases, which is prepared by respectively proliferating four viruses, namely a newcastle disease virus LaSota strain, an infectious bronchitis virus M41 strain, an infectious bursal disease virus BJQ902 strain and a viral arthritis virus T98 strain, on an SPF chicken embryo and a chicken embryo fibroblast to obtain a virus solution, the preparation method comprises the following steps: carrying out 2-4 times concentration, inactivating by using a formaldehyde solution, proportioning according to a certain ratio, emulsifying, preparing an inactivated vaccine, carrying out safety and immune effect tests on the inactivated vaccine by using SPF (specific pathogen free) chickens, carrying out serology and challenge protection tests on the inactivated vaccine, carrying out physical property detection on the prepared inactivated vaccine, and continuously observing for 14 days, according to the present invention, the immune effect test results show that the Newcastle disease antibody level can achieve more than 6.0 log2 after the immunization for 28 d, and the antibody titer after the secondary immunization of the infectious bronchitis is 9 times of the first immunization of the infectious bronchitis;

The invention discloses a group of paired monoclonal antibodies for resisting an infectious bursal disease virus, a quantum dot test strip as well as a preparation method and application of the quantum dot test strip. A mouse is immunized through IBDV-VP2 recombinant protein to obtain monoclonal antibodies 8G10 and 2D2, the 8G10 serves as a capture antibody, the 2D2 serves as a labeled antibody, and the two antibodies can be specifically combined with the infectious bursal disease virus. According to the detection test strip disclosed by the invention, the monoclonal antibody 2D2 marked by quantum dots is adopted as a detection probe, the chicken infectious bursal disease virus antigen can be rapidly, specifically and highly sensitively detected on the basis of a double-antibody sandwich principle, and the lowest detection limit can reach 102.5 TCID50 / mL. The method is suitable for on-site rapid diagnosis, animal quarantine and epidemiological investigation of the infectious bursal disease, and has important application value.

The present invention describes a recombinant herpesvirus of turkeys (rHVT) that can be used as a vector vaccine for poultry against infection and disease from multiple poultry pathogens. Specifically the rHVT expresses an infectious bursal disease virus (IBDV) viral protein 2 (VP2) gene and a Newcastle disease virus (NDV) fusion (F) protein gene from a first and a second expression cassette inserted in the unique small (Us) region, and expresses an avian influenza vims (AIV) haemagglutinin (HA) gene from a third expression cassette inserted in the unique long (UL) region of the genome of said rHVT either between the UL40 and UL41 genes, or between the UL44 and UL45 genes. This rHVT can be used to vaccinate poultry against MDV, IBDV, NDV and AIV.

The invention discloses application of mussel polysaccharide in preparation of a product for treating and / or preventing IBD (infectious bursal disease). The structure of the mussel polysaccharide is alpha-pyranoid glucan formed by glucosidic bonds of (1-> 4)-alpha-D-Glc and (1-> 2)-alpha-D-Glc, the molecular weight is 300-3500 kDa, and the mussel polysaccharide shows remarkable inhibition of DSS-induced body weight loss, diarrhea, hemafecia and other symptoms in an in-vivo model, and recovers the colon length. Moreover, the mussel polysaccharide can also increase the mRNA expression level of an antioxidant gene in the colon of a model animal, inhibit oxidative stress and reduce the loss of tight junction protein. The results indicate that the mussel polysaccharide is an active substance of the IBD prevention and treatment medicine and food, and has value in development and utilization of marine biological resources.

The invention discloses a medicine for treating chicken infectious bursal disease, and belongs to the technical field of veterinary biological products. The invention finds that the PD-1 signaling pathway inhibitor BMS-202 can inhibit the duplication of IBDV (infectious bursal disease virus) of different genotypes and relieve weight inhibition and bursal atrophy caused by IBDV for the first time, new chicken infectious bursal disease treatment drugs can be developed based on BMS-202, and the BMS-202 has important significance in prevention and treatment of IBDV.

The invention also discloses an infectious bursal disease virus propolis live vaccine and a preparation method thereof. The obtained infectious bursal disease virus propolis live vaccine can better induce and regulate host immune response, relieve toxic and side effects of the vaccine and reduce immunosuppression, and meanwhile has the effect of promoting growth. The vaccine product disclosed by the invention can be stably stored, and the virus multiplication capacity of the vaccine is not influenced. The obtained vaccine can replace a traditional live vaccine to be applied to the poultry breeding industry, and the poultry production efficiency can be remarkably improved.