65 results about "Genomic Segment" patented technology

The invention discloses an SNP (Single Nucleotide Polymorphism) marker-based rice disease resistance character whole genome association analysis method, which relates to the technical field of biology, and is characterized by comprising the following steps: selecting a representative genetic recombination population, carrying out high-density SNP genetic typing on the population, and combining recombination event positioning and linkage analysis to obtain the disease resistance character whole genome association analysis of rice. Obtaining genetic parameters reflecting recombination frequencies of different genome segments; on the basis of the obtained original genetic data, parent and offspring genotype information is further integrated, fine positioning and statistics are carried out on recombination breaking points, and a high-resolution genetic map is constructed. By constructing a high-resolution genetic map and combining feature engineering and machine learning, accurate identification and dynamic SNP encryption of a high-recombination region are realized, the marker coverage and associated signal capture capability of the region is enhanced, the functional site leak detection risk is reduced, and the detection efficiency is improved. The positioning accuracy and the molecular breeding application effect of the rice disease resistance character related candidate gene are improved.

The present disclosure encompasses a lipid nanoparticle (LNP) comprising a polypeptide comprising a nucleic acid sequence encoding an engineered meganuclease that binds and cleaves a recognition sequence within a Hepatitis B virus (HBV) genome. Further, the disclosure encompasses pharmaceutical compositions comprising the LNPs, and the use of such compositions for inactivating a pol gene of an HBV genome or an HBV genome fragment in a cell and treating HBV infections or diseases associated with HBV infections.

The application provides a human Helicobacter pylori detection method and kit based on the nature of four-strand DNA oxidoreductase. The method uses isothermal amplification technology, amplifies the genomic fragment containing the four-strand DNA sequence in the human Helicobacter pylori genome, and realizes rapid and visual detection of the human Helicobacter pylori by adding hematin and oxidoreductase substrate for color reaction.

Provided according to one aspect of the present disclosure is a composition for wound healing or tissue regeneration that comprises a genomic DNA fragment extracted from human placenta-derived mesenchymal stem cells and a placental growth factor and which exhibits excellent tissue regeneration ability. Provided according to another aspect of the present disclosure is a preparation method which enables the efficient, biocompatible, economical, and environmentally friendly production of a composition for wound healing or tissue regeneration having excellent tissue regeneration ability.

The application belongs to the technical field of biological information, and particularly relates to a method for locating ancestral haplotype genes for deep hybrid lines. In the application, the ancestral genotype of a sample to be analyzed is obtained, and then a genomic segment significantly associated with a phenotype is obtained. Then, whole genome analysis is performed. Compared with the prior art, the method for locating ancestral haplotype genes for deep hybrid lines can effectively reduce false positives, and can also be used for heterosis analysis, capture of dominant and epistatic effects between genetic markers and the like.

This invention discloses a chikungunya virus genome fragment armored RNA reference, its preparation method, and its application. The preparation process involves the following steps: (1) constructing the prokaryotic expression backbone vector pACYCAR; (2) cloning the CKV genome fragment CKA (nt17-2276); (3) constructing the prokaryotic expression vector pACYCAR-CKA; (4) inducing the expression of armored RNA; and (5) purifying the armored RNA. This invention solves the technical problems of high biosafety risk, poor stability, and insufficient gene fragment coverage in existing CKV nucleic acid detection references. It fills the gap in the lack of high-quality references for CKV detection.

A genome-wide R-loop detection method, in particular, a genome-wide R-loop detection method independent of affinity enrichment and a use. According to the genome-wide R-loop detection method, nucleases are used to perform enzyme digestion on genomic DNA fragments, and R-loop detection can be achieved by performing one-step enzyme digestion simply using a system of Nuclease P1, T5 exonuclease, and Lambda exonuclease. The whole R-loop detection process does not require affinity enrichment or labeling treatment. The method is not only simple and easy to operate, but also can avoid the loss of samples and fragments, thereby reducing the cost and risk of detection, and improving the applicability.

The invention provides an OsSPL17 gene for improving the strength of rice stalks and application of the OsSPL17 gene, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the OsSPL17 gene disclosed by the invention is as shown in SEQ ID NO. 1. According to the invention, a transgenic vector is constructed by cloning a promoter and a genome segment of an OsSPL17 gene, and transgenic rice of which an OsSPL17 self-promoter drives an OsSPL17 self-genome is obtained. Experimental results show that compared with a wild type, the stem thickness, the stem wall thickness and the stem breaking resistance of the transgenic rice are remarkably increased, which indicates that the OsSPL17 plays an important regulation role in rice stem development and can be used for cultivating lodging-resistant rice varieties.

The application provides a porcine parvovirus detection method and kit based on the nature of four-strand DNA oxidoreductase. The method uses isothermal amplification technology to amplify the genomic fragment containing the four-strand DNA sequence in the porcine parvovirus genome, and realizes rapid and visual detection of porcine parvovirus by adding hematin and oxidoreductase substrate for color reaction.

Methods arc provided for making a plant genome having a desired nucleic acid sequence. One example method includes identifying genomic segments of a plant that include at least one desired nucleic acid sequence, using a trained neural network, which is biology-informed through first pre-training connections between a genetic data layer and at least one intermediate layer and second pre-training connection between the at least one intermediate layer and a phenotypic layer. The method also includes recombining the at least one at least one desired nucleic acid sequence with a second nucleic acid sequence to form a target plant genome.

The present invention relates to a methylation biomarker for breast cancer detection, its combination, and its application. The methylation biomarker for breast cancer detection is selected from at least one of 26 genomic fragments with plasma cfDNA methylation modification patterns. The present invention also relates to a kit for detecting the above-mentioned methylation biomarker or its combination. The present invention combines the analysis of the co-methylation characteristics of multiple methylated cytosines on at least one genomic fragment as a biomarker for determining the incidence of breast cancer. Its accuracy is far greater than that of other plasma biomarker detection and can reduce the occurrence of false positives and false negatives in breast cancer detection.

A method of sequencing a target nucleic acid polymer by (a) modifying a target nucleic acid polymer to produce a modified nucleic acid polymer; (b) producing fragments of the modified nucleic acid polymer, wherein the fragments are attached to locations on a solid support surface (c) determining nucleotide sequences from the fragments at the locations; and (d) producing a representation of the nucleotide sequence for the target nucleic acid polymer based on the nucleotide sequences from the fragments and the relative distances between the locations on the solid support surface.

The invention provides systems and methods for determining patterns of modification to a genome of a subject by representing the genome using a graph, such as a directed acyclic graph (DAG) with divergent paths for regions that are potentially subject to modification, profiling segments of the genome for evidence of epigenetic modification, and aligning the profiled segments to the DAG to determine locations and patterns of the epigenetic modification within the genome.

The invention provides a construction method of a high-throughput sequencing library. The method comprises the following steps: carrying out nucleic acid interruption in a single-tube reaction chamber by adopting transposase with a universal joint, adding the universal joint, and then carrying out at least two rounds of ligation reactions in a branched-combination combination manner to introduce different tag combinations into genome fragments of each cell, thereby obtaining a sequencing library. The homogeneity of cell transposase breaking treatment is higher, the nucleic acid tag connection process is easier to operate, the cell flux covered by single sequencing is improved, the cost is lower, and the method is compatible with a single-cell multi-omics sequencing method.

The invention belongs to the field of biological products and the field of nucleic acid vaccines, and particularly relates to a virus (oropravirus)-like particle, preparation and application of the virus (oropravirus)-like particle as a vaccine. The virus-like particles are Oropers virus-like particles, and the virus-like particles contain proteins encoded by M genome segments of OROV strains or proteins encoded by gene segments of the M genome segments and N proteins of the OROV strains; wherein the corresponding sequence can also be a consistency sequence with the similarity of 75% with the corresponding sequence. Meanwhile, the particle VLP is used as a serum binding antibody of an antigen and an evaluation method of a T cell reaction, and the method can be used for detecting a serum lifting method after vaccine immunization. The invention further provides five nucleic acid vaccines for coding different functional domains of the OROV and combinations thereof, the immunogenicity and T cell reaction induced by the nucleic acid vaccines under different design strategies are displayed, strong body fluid and cell reaction is still kept 154 days after immunization, and it is proved that the nucleic acid vaccines can induce lasting and efficient immune reaction, and the immunogenicity and the T cell reaction are not influenced. The method can be used as a technical reserve and has huge clinical significance and application scenarios.

The invention relates to the field of DNA extraction of leather cultural relics, and discloses a method for extracting cattle genomes from vegetable tanned cattle leather cultural relics. The method comprises the following steps: after cleaning the vegetable tanned cow leather cultural relics, dehydrating, freeze-drying and grinding and crushing with liquid nitrogen; metal ions are chelated through an EDTA-Tris detanning reagent to destroy a tannin structure, so that the wrapping on DNA (Deoxyribose Nucleic Acid) is reduced; then, lysate and protease K are used for lysis and digestion, cell membranes are damaged, protein is degraded, and DNA is released; adding absolute ethyl alcohol to precipitate and separate out DNA; and finally, purifying, recovering and purifying the DNA through an adsorption column. The genome DNA extracted by the method is large in fragment, high in purity and stable and reliable in quality.

The present disclosure provides a method for methylation analysis of genomic fragments.

To provide simple and efficient means for predicting the risk of side effects in chemotherapy for pancreatic cancer.SOLUTION: The single nucleotide polymorphism of any of (a) to (c): (a) a single nucleotide polymorphism identified by rs1980576 in APCDD1 L gene or a single nucleotide polymorphism in linkage disequilibrium or genetic linkage with the single nucleotide polymorphism; (b) a single nucleotide polymorphism identified by rs2272761 in R3HCC1 gene or a single nucleotide polymorphism in linkage disequilibrium or genetic linkage with the single nucleotide polymorphism; and (c) a single nucleotide polymorphism identified by rs9425343 in EDEM3 gene or a single nucleotide polymorphism in linkage disequilibrium or genetic linkage with the single nucleotide polymorphism, which are present on the genomic DNA in a biological sample taken from a subject, is analyzed, the genotype for the single nucleotide polymorphism is determined, and based on the determined genotype, the prediction of the risk of side effects when chemotherapy for pancreatic cancer is performed is assisted.SELECTED DRAWING: None

This invention belongs to the field of biotechnology, specifically relating to a Cas9 nuclease and its uses. The Cas9 nuclease (Cas9-R780A) of this invention possesses Cas9 nuclease activity and is suitable for CRISPR / Cas9 systems. The Cas9 nuclease (Cas9-R780A) is obtained by mutating arginine at position 780 of the wild-type Cas9 nuclease to alanine. Using the Cas9 nuclease (Cas9-R780A) to cleave double-stranded DNA produces protruding breaks. Complementary bases to these protruding breaks can be added via ligation, enabling precise editing of specific locations within genomic DNA fragments.

The present invention relates to a method comprising: generating a series of time-based clustering images for a plurality of contiguous conserved library fragments from a genome sample, characterized in that each time-based clustering image in the series is generated sequentially by: introducing, into a flow cell, a respective sample that includes some of the contiguous conserved library fragments, wherein some of the contiguous conserved library fragments are attached to a solid support or are attached to each other; initiating the release of some of the contiguous conserved library fragments from the solid support or from each other; amplifying some of the contiguous conserved library fragments to generate a plurality of respective template strands; having the respective template strands; and taking images of the respective template strands.

The invention relates to the technical field of microbiological detection, in particular to a primer pair, a kit and a method for detecting brassica rapa brassicae. On the basis of an EmDEA technology, a primer pair and a primer probe combination which have the best comprehensive detection effect on the brassica rapa brassicae are obtained through analysis and optimization of a strain Pb3PbPT3Sc00006 genome segment of the brassica rapa brassicae, and the detection method for the brassica rapa brassicae is established and has the advantages of high specificity, low detection limit and high detection sensitivity. And the method is simple and convenient to operate, relatively low in experimental condition requirement and relatively short in time consumption, is suitable for port daily import and export detection and outdoor on-site monitoring requirements, and has a relatively good application prospect.

A genome editing composition conjugated to a dendrimer for safe and efficient cell targeting and intracellular delivery of a genome editing system has been established. Compositions and methods for genome editing and gene regulation in one or more diseases and disorders are described. The compositions include a gene editing system, preferably a CRISPR / Cas system conjugated to a dendrimer. In a preferred embodiment, the dendrimer is covalently conjugated to a Cas9 nuclease that is further complexed with a specific single-guide RNA for targeting a genomic segment of a target cell.

This application provides a method and kit for detecting pathogenic microorganisms based on the properties of tetrastranded DNA oxidoreductase. The method utilizes isothermal amplification technology to amplify genomic fragments containing tetrastranded DNA / RNA sequences in the pathogenic microorganism genome, and then performs a colorimetric reaction by adding heme and oxidoreductase substrates, achieving rapid and visual detection of pathogenic microorganisms.

The present invention relates to a primer pair and its application in the quality control method of BCG-CpG-DNA adjuvant. The present invention first uses the BCG genomic DNA fragment itself as a quality control fragment, verifies its feasibility, amplifies the quality control fragment with specific primers, analyzes the amplification results, and thus conducts quality control on the BCG-CpG-DNA adjuvant.

Primers are provided that are capable of (i) specifically hybridizing to a genomic DNA fragment comprising an adaptor, or an amplicon thereof, and (ii) selectively amplifying GC-rich sequences of a genome. The primers can be employed to interrogate the methylation status of multiple gene regulatory sequences in parallel, e.g., to identify aberrantly methylated or demethylated gene regulatory sequences on a global genomic scale. Methods that utilize, and compositions and kits that include, such primers are also described.

This disclosure provides technology for ordering sequence information derived from one or more target polynucleotides. In one aspect, one or more tiers or levels of fragmentation and aliquoting are generated, after which sequence information is obtained from fragments in a final level or tier. Each fragment in such final tier is from a particular aliquot, which, in turn, is from a particular aliquot of a prior tier, and so on. For every fragment of an aliquot in the final tier, the aliquots from which it was derived at every prior tier is known, or can be discerned. Thus, identical sequences from overlapping fragments from different aliquots can be distinguished and grouped as being derived from the same or different fragments from prior tiers. When the fragments in the final tier are sequenced, overlapping sequence regions of fragments in different aliquots are used to register the fragments so that non-overlapping regions are ordered. In one aspect, this process is carried out in a hierarchical fashion until the one or more target polynucleotides are characterized, e.g. by their nucleic acid sequences, or by an ordering of sequence segments, or by an ordering of single nucleotide polymorphisms (SNPs), or the like.

The invention discloses a recombinant herpesvirus turkey strain for co-expressing an H9 subtype AIVHA gene and ILTVgB, gD and gI genes as well as a construction method and application of the recombinant herpesvirus turkey strain, and belongs to the technical field of medicine or veterinary medicine. The recombinant herpesvirus of turkeys is obtained by co-transfecting CEF cells with recombinant clay containing H9 subtype AIVHA genes and ILTVgB genes, recombinant clay containing ILTVgD and gI genes and parent clay containing HVT genome DNA fragments and then carrying out virus rescue. The recombinant herpesvirus of turkeys is prepared by the following steps: cotransfecting CEF cells with recombinant clay containing H9 subtype AIVHA genes and ILTVgB genes, recombinant clay containing ILTVgD and gI genes, and parent clay containing HVT genome DNA fragments. The recombinant virus strain obtained by the invention has good in-vitro replication ability and genetic stability. After SPF chickens are immunized, the SPF chickens can obtain immune protection against H9 subtype AIV and ILTV virulent viruses. The invention provides a new technical means for simultaneously preventing the H9 subtype avian influenza and the avian infectious laryngotracheitis.

The invention discloses a sequencing library construction method and application of the sequencing library construction method in genome typing and high-density physical map construction. The method comprises the following steps: respectively connecting genome DNA fragments covering the whole genome of a target species to a cloning vector, then carrying out enzyme digestion by using restriction endonuclease, screening fragments of which the length is greater than the length of the cloning vector from the enzyme digestion fragments, and adding ligase to carry out catalytic connection to obtain a self-ligation product; carrying out PCR (Polymerase Chain Reaction) amplification by using the primer pair self-ligation product as a template, wherein an obtained PCR product is a genome sequencing library of the target species; in combination with a PacBio HiFi sequencing technology, the method provided by the invention omits the processes of BAC library construction and monoclonal selection, and the marker generation and genomic typing accuracy are significantly improved. The method can be applied to development of high-resolution bin markers of polyploidy species and genome assembly quality, and can promote breeding and improvement of polyploidy crops.

The invention discloses a chromosome outer circular DNA sequence identification method based on deep learning, and relates to the technical field of genome biological information, and the method comprises the following steps: obtaining a known chromosome outer circular DNA sequence as a positive sample; randomly selecting a genome segment as a negative sample, wherein the overlapping degree of the genome segment and any known chromosome outer circular DNA region is lower than 20% from the reference genome according to an equal length distribution and chromosome distribution principle; cleaning and standardizing the positive and negative samples, and dividing the positive and negative samples into a training set, a verification set and a test set; converting the positive and negative sample sequences into feature representation which can be processed by a neural network through a multi-modal coding strategy, and training a one-dimensional convolutional neural network architecture by using a training set; when the number of the training samples is insufficient, introducing a deep convolutional generative adversarial network to perform data enhancement; and processing a DNA sequence to be detected through a multi-modal coding strategy, inputting the processed DNA sequence to the trained model, outputting a probability score of existence of the circular DNA outside the chromosome, and completing classification judgment according to a preset threshold value.

The present disclosure provides a method for characterizing a genomic locus that can comprise providing a quantity of genomic DNA having a locus of interest therein; contacting the genomic DNA with a transposase, which transposase fragments the genomic DNA and appends transposon DNA on a terminus of the fragments, thereby creating tagmented DNA fragments; performing a PCR reaction utilizing (i) the tagmented DNA fragments; (ii) a first primer that anneals to a portion of the transposon DNA; and (iii) a second primer that anneals to a portion of the locus of interest; and sequencing a product of the PCR reaction.