146 results about "Genotyping by sequencing" patented technology

The invention provides a KASP molecular marker related to a corn ear height character under a drought condition and application of the KASP molecular marker, and belongs to the technical field of molecular genetics. The SNP marker site related to the ear height character under the drought condition and having polymorphism is obtained through whole genome association analysis, the SNP marker site refers to a corn B73 RefGenv4 genome, when nucleotide polymorphism T / C exists at the position 147243591 of a 10 # chromosome, the SNP marker site is a dominant character, and nucleotide sequences used for amplifying a KASP molecular marker primer group are shown as SEQ ID NO.1-3. Gene typing identification can be rapidly, simply and efficiently carried out by utilizing a KASP typing technology, enzyme digestion and electrophoresis are not needed, accurate identification and selection can be carried out in a seedling stage, a large amount of breeding workload can be reduced, the period of high-ear-position breeding selection can be shortened, and the method can be used for high-ear-position molecular marker-assisted breeding of corn.

The invention relates to the technical field of molecular genetic breeding, in particular to a KASP molecular marker related to salt tolerance in a mung bean germination period, a primer group, a kit and application. According to the KASP molecular marker Chr126769484 related to the salt tolerance in the mung bean germination period, primers are designed according to the KASP molecular marker, typing is carried out by utilizing specific matching of basic groups at the tail ends of the primers, the KASP molecular marker related to the salt tolerance in the mung bean germination period is developed, genetic typing is carried out, identification is accurate and convenient, the salt tolerance in the mung bean germination period can be predicted at low cost, and the application prospect is wide. The process of salt-tolerant variety breeding in the mung bean germination period is simplified, the breeding efficiency is improved, and the mung bean breeding process is accelerated.

Rosa chinensis gray mold is a main disease in the cultivation process of rosa chinensis, and seriously threatens the quality and yield of cut-flower rosa chinensis. In order to effectively cope with the disaster stress which influences the yield and quality of the Chinese rose, 192 pairs of SSR primers are used in the research to carry out PCR amplification on DNA of parents' Jinmai '' Beijing red 'and 6 randomly selected offspring. 35 pairs of primers show genetic difference between male and female parents. The 35 pairs of primers are further used for carrying out PCR amplification on filial generation DNA, detection is carried out through PAGE electrophoresis, and an electrophoretic band is converted into a 0, 1 matrix for genetic typing. The method comprises the following steps: selecting an optimal subset through correlation regression analysis, and finally successfully screening out three primers, namely, Geno-BC-1, Geno-BC-2 and Geno-BC-3, which are related to gray mold resistance. The discovery provides an important theoretical basis for molecular marker-assisted Chinese rose disease-resistant breeding, and is expected to promote early identification of disease-resistant plants, so that the breeding period is shortened, and the breeding efficiency is improved.

A method for sequencing can be used to monitor a recipient of an allograft. A sample comprising nucleic acid molecules can be provided from the recipient of the allograft. A plurality of nucleic acid molecules can be isolated from the sample and amplified. The plurality of nucleic acid molecules can be subjected to one or more amplification reactions using a plurality of primers to generate a plurality of DNA molecules. The plurality of primers can target at least 100 independent polymorphisms. The DNA molecules, or derivatives thereof, can be sequenced. The method may not require genotyping of the recipient.

The present invention provides a molecular marker, a detection method and an application thereof that affect the quality of sheep meat, and belongs to the technical field of molecular marker-assisted breeding. The present invention provides a molecular marker related to the tenderness of sheep meat, the molecular marker comprises a sequence shown in SEQ ID No.1, and a polymorphic site A>G exists at the 33rd site of the 3'UTR region. The molecular marker of the present invention is significantly correlated with the tenderness of sheep meat, and the molecular marker can be used to accurately perform genotyping, thereby improving the accuracy and reliability of genetic evaluation, and facilitating the early screening of sheep individuals with excellent meat quality, thereby increasing the speed of breeding superior meat sheep and saving feeding costs.

The invention discloses a western China cattle parameter-free Bayesian genome selection method. The method comprises the following steps: step 1, constructing a reference group and measuring important classification characters; step 2, collecting blood from the western China cattle, preserving, extracting DNA, and performing genetic typing to obtain the genotype of each SNP site; step 3, for the individual Chinese western cattle, using plink2 software to associate the genotype file and the phenotype file to obtain summarized statistical data; partitioning each SNP site according to the position of a chromosome to obtain an LD reference file; 4, the summarized statistical data and the LD reference file serve as input files of a parameter-free Bayesian model, and an effect value of the SNP site is obtained through calculation; and multiplying the genotype vector of the candidate individual by the locus effect value vector to obtain a genome estimation breeding value. The invention aims to establish a breeding method for classification characters of the Huaxi cattle based on a parameter-free Bayesian genome selection technology, and provides a molecular breeding method for breeding high-quality Huaxi cattle so as to promote rapid development of beef cattle breeding industry.

The invention discloses a method for identifying Yushan black pigs by adopting an SNP (Single Nucleotide Polymorphism) molecular marker technology, and particularly relates to the technical field of molecular detection.The method comprises the following steps: S1, sample preparation: collecting any one of ear tissues, tail tips or blood samples of to-be-detected pig individuals, and extracting to obtain genome DNA (Deoxyribose Nucleic Acid); the genome DNA is used as a template, amplification detection is conducted through a first primer pair, a second primer pair, a third primer pair and a fourth primer pair corresponding to the first SNP site, the second SNP site and the fourth SNP site, and the nucleotide sequences of the first primer pair, the second primer pair and the fourth primer pair are sequentially SEQ ID NO.5-SEQ ID NO.12. By detecting the specific single nucleotide polymorphic sites in the Yushan black pig genome and carrying out genetic typing comparison, the Yushan black pigs and the filial generation thereof can be quickly and accurately identified.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker associated with a pig backfat thickness character and application. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO: 1; one SNP molecular marker site A / G is arranged at the 254bp position of the sequence of the SNP molecular marker. Genetic typing is carried out by utilizing a gene chip technology, whole genome association analysis is carried out in combination with the character of correcting the 100 kg backfat thickness of a large white pig, one SNP molecular marker remarkably related to the character is screened out, and a new marker resource is provided for molecular breeding of the growth character of the breeding pig.

The invention relates to a Yangtze river globefish polymorphic SNP molecular marker as well as a development method and application thereof, and belongs to the technical field of DNA molecular markers, nucleotide sequences of sites of the Yangtze river globefish polymorphic SNP molecular marker are as shown in SEQ ID NO: 1-NO.19, primers for amplifying the sites of the SNP molecular marker are as shown in Snpp 01-57, and the primers for amplifying the sites of the SNP molecular marker are as shown in SEQ ID NO: 1-SEQ ID NO: 2-SEQ ID NO: 3-SEQ ID NO: 4. The SNP molecular markers provided by the invention are all derived from high-quality chromosome-level genomes of the Yangtze-River cowfish, screening is carried out in combination with re-sequencing data of three geographical populations, polymorphic SNP loci in the genomes of the Yangtze-River cowfish can be more comprehensively detected, and by utilizing a KASP genotyping technology, the SNP molecular markers can be used for detecting the genomes of the Yangtze-River cowfish. Genetic typing can be efficiently and accurately performed on the polymorphic SNP sites of the Yangtze river globefish, the detection cost is remarkably reduced, and an effective genetic molecular marker is provided for population genetics research such as genetic diversity evaluation, genetic differentiation analysis and genetic relationship identification of the Yangtze river globefish and the sibling species of the Yangtze river globefish.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to disease resistance of ricefield eel and application of the SNP molecular marker, and relates to the technical field of molecular biology. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO.3, and SNP sites, namely G / A mutation and A / C mutation, exist at the 143rd and 413th basic groups of the SNP molecular marker. According to the invention, SNP loci in LamR genes in a ricefield eel group are screened out through sequence alignment, and genotyping is carried out on the SNP loci; and comparing the differences of the disease resistance of the genotypes, and screening to obtain the genotype with the strongest disease resistance, thereby providing an effective molecular marker for detecting the disease resistance of the ricefield eel. The SNP molecular marker provided by the invention is associated with the disease resistance of the ricefield eel, and provides effective technical support for breeding of disease-resistant ricefield eel varieties.

The invention belongs to the technical field of breeding, and particularly relates to a fine-wool sheep genome selective breeding method. According to the method, genetic typing is carried out on each sample by using a fine-wool sheep 50K SNP liquid phase chip, statistical analysis is carried out through genetic typing and phenotype data, RRBLUP and SSGBLUP are used for training population genotype data and phenotype data and calculating prediction accuracy, and finally, the net wool amount, wool length, wool fiber diameter and weight are selected as four breeding target characters. A genome breeding value (GEBV) of the weight, the wool length, the wool fiber diameter and the net wool amount of the fine-wool sheep is used as a denominator, the standard economic value of each character is used as a molecule, a fine-wool sheep genome comprehensive selection index estimation model (GPI) is formulated, and selective breeding of the fine-wool sheep genome is performed according to the GPI numerical value. A genome selection strategy with high accuracy and low cost is provided, and the method has important significance on high-quality development of fine-wool sheep breeding industry and industry.

The invention belongs to the technical field of pig molecular markers, and particularly discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to a pig weaning litter number character and application of the SNP molecular marker. Gene typing is carried out by utilizing a gene chip technology, whole genome association analysis is carried out on the basis of SNP molecular markers, and 11 SNP molecular marker sites related to the weaning litter number character of the binary hybrid sow are screened out through the analysis. The sites of the SNP molecular marker are located on a WDR25 gene segment and a BEGAIN gene segment of a pig chromosome 7, and the SNP molecular marker can be used for marker-assisted breeding of the pig weaning litter number character.

The invention relates to an HLA (human leukocyte antigen) genotyping method, device, equipment and medium. The method comprises the following steps: constructing a polymorphic database, and generating an SNP feature matrix of the polymorphic database; obtaining HLA genotype data of a to-be-detected sample, and reconstructing the HLA genotype data of the to-be-detected sample to obtain a feature vector of the to-be-detected sample; two alleles of the same gene locus are obtained from the polymorphism database, all heterozygous combinations of the two alleles are generated, mononucleotide diversity characteristics in all the heterozygous combinations are combined, and an expected characteristic set of all the heterozygous combinations is obtained; comparing the feature vector of the to-be-tested sample with the expected feature set bit by bit, counting mismatch records of data in the feature vector of the to-be-tested sample and the expected feature set, and recording the mismatch number of each heterozygous combination; and determining the heterozygous combination with the minimum mismatch number as an HLA typing result. By adopting the method, genotyping can be efficiently and accurately carried out.

The invention discloses a reagent, a detection system and a method for HPA genotyping detection of Chinese population. According to the invention, a multiplex PCR technology based on a fluorescent probe melting curve is adopted, specific primers of different HPA systems and fluorescent labeled probes are arranged and combined, and finally, only three reaction tubes are used for simultaneously carrying out genotyping on 14 HPA antigen systems of Chinese population at a time. In addition, the detection system disclosed by the invention is simple, convenient, rapid, accurate and efficient, and has a relatively high practical value.

The invention discloses an SNP molecular marker combination related to disease resistance of ricefield eels and application of the SNP molecular marker combination, and relates to the technical field of molecular biology. The SNP molecular marker combination comprises the following molecular markers (1)-(3): (1) a molecular marker with a nucleotide sequence as shown in SEQ ID NO.7; (2) a molecular marker with a nucleotide sequence as shown in SEQ ID NO.8; and (3) a molecular marker with a nucleotide sequence as shown in SEQ ID NO.9. According to the invention, an SNP locus in a TLR7 gene in a monopterus albus group is screened through sequence alignment, and genotyping is carried out on the SNP locus; and comparing the differences of the disease resistance of the genotypes, and screening to obtain the genotype with the strongest disease resistance, thereby providing an effective molecular marker for detecting the disease resistance of the ricefield eel. The molecular marker combination provided by the invention is associated with the disease resistance of the monopterus albus, and provides effective technical support for breeding of disease-resistant monopterus albus varieties.

The invention discloses a carrot hybrid purity identification method and an InDel primer combination used by the same, and belongs to the field of hybrid purity identification. The InDel primer combination provided by the invention is composed of 10 primer groups; ten InDel sites are correspondingly amplified respectively, so that each InDel site is subjected to genetic typing or homozygosis or heterozygosis determination respectively. The primer combination can be used for identifying the purity of the carrot hybrid, and early identification can be carried out in the seed or seedling stage of the carrot hybrid, so that the purity of the hybrid is ensured, and the legitimate rights and interests of producers and breeders are effectively maintained.

The invention discloses RT-qPCR-HRM (real-time quantitative polymerase chain reaction-high-resolution melting) detection primers and a detection method for identifying five different subtype avian influenza viruses, and belongs to the technical field of biology. The invention discloses an RT-qPCR-HRM detection method for identifying five different subtypes of avian influenza viruses, which comprises the following steps: designing a specific primer based on a highly conserved region of an AIV M gene, and simultaneously carrying out genetic typing on five subtypes of AIV strains H3, H5, H6, H7 and H9 in combination with an HRM technology; by comparing melting curve characteristics of M gene segments of different subtypes of AIV, an AIV detection method with good sensitivity and specificity is established. According to the method, the five AIVs of H3, H5, H6, H7 and H9 can be accurately typed, the defect of missing detection of a traditional AIV detection method is overcome, and the method has a wide application prospect.

The invention belongs to the technical field of pig molecular markers, and particularly discloses an SNP molecular marker related to the character of the number of healthy piglets born by a sow and application of the SNP molecular marker. Genetic typing and whole genome association analysis are carried out on 732 large white sows by using second-generation gene sequencing and gene chip technologies, an SNP site significantly related to the character of the number of healthy piglets born by the large white sows is screened out, the SNP site is located at the upstream of an SLC22A12 gene of a pig chromosome 2 and corresponds to the 7565970th nucleotide of a chromosome 2 of an international pig 11.1 version reference genome, and the SNP site is located at the upstream of the SLC22A12 gene of the chromosome 2 of the pig 11.1 version reference genome and is located at the upstream of the SLC22A12 gene of the chromosome 2 of the pig 11.1 version reference genome. The method can be used for character prediction of healthy litter size of sows or auxiliary breeding.

The present disclosure provides methods and systems for classifying microsatellite and minor alleles in a sample. Also, the present disclosure provides methods and systems for generating classifiers for conditions based on microsatellite loci and for performing pan-cancer assays. The methods and systems can involve next-generation sequencing of nucleic acid samples from subjects and genotyping microsatellite loci in the samples.

The application belongs to the technical field of pig molecular marker, and particularly discloses a SNP molecular marker related to the litter size of sows and application thereof. SLC22A12 A SNP site significantly related to the litter size of Landrace sows is screened out by using second-generation gene sequencing and gene chip technology for genotyping and whole genome association analysis of 732 Landrace sows, the SNP site is located on the upstream of a gene on chromosome 2 of pigs, corresponding to the 7565970th nucleotide on chromosome 2 of the international pig 11.1 version reference genome, and can be used for predicting or assisting breeding of the litter size of sows.

The invention discloses a dCAPS molecular marker co-segregated with a cucumber fruit length gene and its application, and belongs to the field of vegetable molecular marker assisted breeding. The dCAPS molecular marker includes a forward primer whose sequence is shown in SEQ ID NO:1 and a reverse primer shown in SEQ ID NO:2, or further includes a PstI endonuclease. The dCAPS molecular marker co-segregates with the cucumber fruit length gene during the cucumber molecular marker assisted breeding process, that is, the phenotypes of long-fruit and short-fruit individual plants in the population are respectively consistent with the long-fruit and short-fruit parent band types amplified by the dCAPS molecular marker, and the heterozygous band type amplified by the marker corresponds to a long-fruit type plant. The dCAPS molecular marker is used to perform genotyping of the plant at the seedling stage, and the length of the fruit of each individual plant is predicted based on the typing result, so that molecular marker assisted breeding can be performed at the seedling stage to improve breeding efficiency.

The invention discloses an SWEET15A01 gene promoter and application of Indel variation of the SWEET15A01 gene promoter in improvement of cottonseed oil content, the promoter has multiple variations between low-oil upland cotton Hubei cotton 22 (E22) and high-oil sea island cotton 3-79, resulting in differential expression of the gene in the later stage of ovule development. A transgenic material created by driving the gene expression by using a gossypium barbadense genotype promoter significantly increases the oil content of the seeds, and has no significant influence on fiber yield and quality related traits. A molecular marker is developed aiming at Indel variation of 21bp, genetic typing can be carried out on gossypium barbadense and upland cotton populations, and high-oil cotton germplasm and low-oil cotton germplasm can be distinguished. The molecular marker provided by the invention has important application value in the aspects of cotton molecular marker-assisted breeding and high-oil germplasm innovation.

The invention discloses a molecular marker related to disease resistance of ricefield eel and application of the molecular marker, and relates to the biological field of aquaculture and molecular marker screening. According to the invention, SNP loci in SFRP2 and TC1A gene coding regions in a ricefield eel group are screened through sequence alignment, and the two genes and a combination thereof are subjected to genetic typing; and comparing the differences of the disease resistance of the genotypes, and screening to obtain the genotype with the strongest disease resistance and the combination thereof, thereby providing an effective molecular marker for detecting the disease resistance of the ricefield eel. According to the monopterus albus disease resistance screening method developed based on the molecular marker, monopterus albus varieties with excellent disease resistance can be screened by utilizing SNP differences of disease-resistant genes, and then the monopterus albus varieties are used for disease resistance breeding. Effective technical support is provided for breeding of disease-resistant ricefield eel varieties.

This invention discloses a reagent, detection system, and method for HPA genotyping in the Chinese population. The invention employs a multiplex PCR technique based on fluorescent probe melting curves, arranging and combining specific primers and fluorescently labeled probes for different HPA systems. This ultimately enables simultaneous genotyping of 14 HPA antigen systems in the Chinese population using only three reaction tubes in a single test. Furthermore, the detection system of this invention is not only simple and rapid but also accurate and efficient, possessing high practical value.

The invention relates to methods of identifying and characterizing a Cannabis spp. plant with respect to a flower to leaf ratio trait comprising genotyping the plant for a quantitative trait locus (QTL) associated with a flower to leaf ratio trait, and to methods of producing plants having a flower to leaf ratio trait of interest based on defined allelic states of polymorphisms defining the QTL. Also provided are Cannabis spp. plants having a flower to leaf ratio trait of interest comprising defined allelic states of polymorphisms defining the QTL and plants identified, characterized or produced by the methods described herein. The invention further relates to marker assisted selection and marker assisted breeding methods, in particular using a combination of specific markers provided, for obtaining plants having a flower to leaf ratio trait of interest or for modulating the flower to leaf ratio of cannabis plants.

The invention relates to the technical field of molecular detection, and particularly discloses a police dog 10K liquid phase chip, a design method and application thereof and a kit, and genetic typing sites of the police dog 10K liquid phase chip comprise 10463 SNP sites; the physical positions of the 10463 SNP loci are determined based on the comparison of the whole genome sequence of the canine reference genome CanFam 3.1, and the 10463 SNP loci are shown in the table 1. The 10463 SNP loci disclosed by the invention are obtained by screening based on a DNA sample of the police dog, and the obtained 10463 SNP loci can be used as a basis for genetic typing of the police dog; the liquid chip designed according to 10463 SNP loci can quickly realize genotyping of the target SNP loci, and obtained genotyping data can be used for variety identification of police dogs and population diversity analysis of police dogs.

The application discloses a high-throughput KASP molecular marker significantly related to plant height of Gossypium hirsutum and application, and belongs to the technical field of cotton molecular breeding. The KASP marker is successfully developed based on cotton plant type related genes and resequencing data of germplasm resources. Through genotyping and phenotype correlation analysis in 246 natural Gossypium hirsutum populations, four KASP markers (PH-TK1-PH-TK4) significantly related to plant height in three environments are screened. The regulation effect and aggregation effect of the markers on plant height are verified in a separation population. Experiments prove that the KASP marker can be used for high-throughput and accurate genotyping of plant height in early cotton breeding, and provides an effective tool for molecular marker assisted selection of cotton ideal plant type, and helps to accelerate the breeding process of cotton varieties suitable for mechanical harvesting.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker associated with correction of a 100kg backfat thickness character of a pig and application of the SNP molecular marker. The SNP molecular marker is located on a pig chromosome 2 and contains six SNP molecular marker sites. Genetic typing is carried out by utilizing a gene chip technology, whole genome association analysis is carried out in combination with the 100kg backfat thickness correction character of the landrace, six SNP molecular markers obviously related to the character are screened out, and a new marker resource is provided for molecular breeding of the growth character of the boar.

The invention discloses a quantitative trait locus related to soybean isoflavone content and an application thereof, and provides a soybean molecular marker-assisted breeding method based on a high-density genetic linkage map. A cultivated soybean variety JD12ZDD23040 is selected to be hybridized with a wild soybean variety Y9ZYD02739, and a control group consisting of 185 recombinant inbred lines (RILs) from F8 sources is constructed by a single seed descent SSD method; genotyping is performed by sequencing and SNP calling, and map construction, QTL detection and QTL integration analysis are performed; based on statistical analysis, an integrated QTL significantly related to the soybean isoflavone content trait is obtained; based on the obtained integrated QTL, a molecular marker and a primer thereof are developed for soybean molecular marker-assisted breeding related to the soybean isoflavone content trait.

The invention relates to the field of plant genetic breeding, and discloses an SNP molecular marker related to corn drought resistance and application of the SNP molecular marker. Two markers ZMDRI291441904 and ZMDRI159118475 which are obviously associated with the corn drought tolerance index are mined through whole genome association analysis, the marker sites are A / G and T / C respectively, the two markers are used for performing genetic typing on the corn inbred line, and the drought tolerance indexes of the corn inbred line of the two genotypes of each marker respectively follow the following conditions: GGgt, GGgt, GGgt, GGgt, GGgt, GGgt, GGgt, GGgt, GGgt, GGgt, GGgt, GGgt, GGgt, GGgt, GGgt, GGgt, , AA, CCgt; tT. Experiments show that the maize inbred line with relatively high drought tolerance index can be found by detecting the SNP site. The invention provides a new method for corn molecular marker-assisted selective breeding, and has important significance in drought-resistant screening of corn inbred lines and breeding of drought-resistant new varieties.