Composite STR detection method with improved resolving ability in Chinese crowd and kit

A technology of identification ability and determination method, which is applied in the field of genetic analysis to detect polymorphisms of short tandem repeat sequences, and can solve problems such as the influence of DNA testing technology efficiency, low genetic polymorphism of loci, and large differences

Inactive Publication Date: 2009-04-01
APPLERA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even among the 13 core loci recommended by the FBI in the United States, some loci have low genetic polymorphisms, or have large differences among different populations, which has a certain impact on the application and efficiency of DNA testing technology

Method used

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  • Composite STR detection method with improved resolving ability in Chinese crowd and kit
  • Composite STR detection method with improved resolving ability in Chinese crowd and kit
  • Composite STR detection method with improved resolving ability in Chinese crowd and kit

Examples

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Comparison scheme
Effect test

Embodiment 1

[0081] Example 1 Compound STR Determination Method for Chinese Population

[0082] The kit used in this example is an improved Identifiler TM PCR amplification kit, Sinofiler multiplex PCR kit, in which D6S1043 and D12S391 are used to replace the original TH01 and TPOX. Experimental reagents, experimental instruments, experimental operations and data analysis were carried out in accordance with the operation manual provided by the manufacturer (same as Identifiler TM PCR Amplification Kit User Manual, Cat. No. 4323291). Experimental details are described in detail below.

[0083] PCR reaction components

[0084] Component Vendor Concentration range Tris-HCl Invitrogen, Roche, Sigma-Aldrich 8-20mM KCl Invitrogen, Roche, Sigma-Aldrich 40-75mM MgCl2 Invitrogen, Roche, Sigma-Aldrich 1.2mM-2.4mM BSA Invitrogen, Sigma-Aldrich, Promega 50-180ug / ml dNTP Invitrogen, Qiagen, Roche 0.1mM-0.25mM each Enzymes Invi...

Embodiment 2

[0100] Example 2 The improved discrimination ability of the composite STR assay method of the present invention for the Chinese population Identifiler TM The identity probability (probability of identity, PI) of the 15 STR loci in the PCR amplification kit in different populations. The matching probability (matching probability) in the Chinese population is higher than that of African Americans, Caucasians and Hispanic Americans, suggesting that Identifiler TM The PCR kits had lower discriminatory power in Chinese population than in other populations.

[0101]

[0102] The inventors then use the improved Identifiler TMThe PCR amplification kit, Sinofiler multiplex PCR kit, analyzed 176 unrelated Chinese DNA samples according to the method described in Example 1. The combined identity probability of the 15 STR loci in the Sinofiler kit is 1.01x10 in the Chinese population -18 . This result shows that the Sinofiler composite STR assay has improved discriminatio...

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Abstract

The invention discloses a composite STR determination method with improved resolution capability in Chinese people, in particular a method for carrying out genotyping to the Chinese people. The method comprises synchronous amplification and the analysis of the locus group consisting of the following loca: D8S1179, D21S11, D7S820, CSF1PO, D3S1358, D13S317, D16S539, D2S1338, D19S433, vWA, D18S51, AMEL, FGA, D5S818, and at least one of D6S1043 and D12S391. The invention also discloses a kit and a primer applied to the method.

Description

technical field [0001] The present invention generally relates to genetic analysis methods for detecting short tandem repeats (STR) polymorphisms in individuals. Specifically, the present invention relates to a composite STR assay method and kit with improved discrimination in Chinese population. Background technique [0002] Short tandem repeat (STR) is a highly polymorphic genetic marker in the human genome. PCR-STR DNA genotyping technology has achieved remarkable results after being applied to forensic identification; and its detection method is based on Multiple site amplification detection is the most popular among experimental technicians. Its equipment is simple, it uses less sample material, and the method is easy to implement. It can obtain more polymorphism information in a short period of time. Especially in the establishment of DNA database, STR compound amplification technology has great advantages. The US FBI selected 13 STR loci for the establishment of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 洛里·亨尼西张倩维
Owner APPLERA
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