5 results about "Str loci" patented technology

This invention belongs to the field of genetics and provides a technique and data analysis method for simultaneously detecting 193 Y-STRs in the Chinese population based on an NGS platform. This invention discloses 193 Y-STR loci for human Y-chromosome STR genotyping; the selected 193 Y-STRs are used for library construction using a two-step PCR method. This invention has advantages such as rapid amplification, consistent efficiency, high sensitivity, and good data visualization; due to the high depth of high-throughput sequencing, it is more conducive to the analysis of mixed and degraded materials.

The present application relates to the technical field of sex chromosome STR identification, and particularly relates to an X chromosome STR locus composite amplification primer group, a 9-color fluorescent label composite amplification kit and application thereof.The composite amplification primer group comprises 45 pairs of specific amplification primer pairs, the 45 pairs of specific amplification primer pairs include 44 X chromosome STR loci and 1 pair of specific amplification primer pairs of a gender locus AMEL, and the nucleotide sequences of the primer pairs in the composite amplification primer group are shown as SEQ ID NO.1-90.The specificity of each primer in the composite amplification primer group is relatively strong, no primer dimer or interaction is generated during composite PCR amplification, the amplification efficiency difference of each pair of primers is relatively small, and the identification sensitivity is relatively high.The composite amplification primer group provides a molecular basis and core necessary reagent for simultaneously detecting 44 X-STR loci at a time.

The invention discloses a kit for pigeon individual recognition and paternity identification typing and application. The invention provides 16 novel STR loci for pigeon individual identification and paternity identification typing, and the STR loci are all tetranucleotide and pentanucleotide repeated loci. The kit established on the basis of the new STR site is clear, simple, convenient and accurate in typing result interpretation, the cumulative discriminability of the kit reaches 0.999999999999999999999999999999720944, and the combined exclusion probability of the kit reaches 0.9999999999720944. The individual identification capability is high, the genetic relationship identification is reliable, and a guarantee is provided for breeding of excellent racing pigeon varieties and genetic relationship identification.

The invention discloses a freeze-drying eight-color composite amplification kit with high inhibition resistance and application, and belongs to the field of molecular biology, a composite amplification system provided by the invention comprises specific primers for amplifying 31 gene loci, and the 31 gene loci comprise specific amplification primers of 29 STR gene loci, one sex assisting site Y indel and one Amelogenin site. The kit disclosed by the invention has the advantages of being rapid and efficient in detection of complex detection materials containing high inhibitors in on-site cases; all gene loci are compatible with an existing case database; and the time of case checking is shortened, the public security officers are helped to quickly lock the suspects, and the case cracking efficiency is improved.

The invention provides a primer group, a kit, an amplification system and a freeze-drying material for simultaneously amplifying 25 human STR gene loci and a preparation method of the freeze-drying material. The primer group capable of simultaneously amplifying the 25 STR gene loci of the human comprises nucleotide sequences as shown in SEQ ID NO.1 to SEQ ID NO.50, and the primer group can simultaneously amplify and detect the 25 STR gene loci and realize efficient and specific detection on the 25 STR gene loci; meanwhile, the freeze-dried material for amplifying the 25 human STR loci is convenient to store and use, the amplification efficiency, the amplification balance and the amplification sensitivity of the freeze-dried material are still kept at the original level, and the use requirements of users are better met.