The present application relates to the technical field of sex
chromosome STR identification, and particularly relates to an
X chromosome STR locus composite amplification primer group, a 9-color fluorescent
label composite amplification kit and application thereof.The composite amplification primer group comprises 45 pairs of specific amplification primer pairs, the 45 pairs of specific amplification primer pairs include 44
X chromosome STR loci and 1 pair of specific amplification primer pairs of a gender locus AMEL, and the
nucleotide sequences of the primer pairs in the composite amplification primer group are shown as SEQ ID NO.1-90.The specificity of each primer in the composite amplification primer group is relatively strong, no
primer dimer or interaction is generated during composite PCR amplification, the amplification efficiency difference of each pair of primers is relatively small, and the identification sensitivity is relatively high.The composite amplification primer group provides a molecular basis and core necessary
reagent for simultaneously detecting 44 X-
STR loci at a time.