20 results about "Str loci" patented technology

The invention discloses an STR (short tandem repeat) fluorescence labeling composite amplification system, the STR fluorescence labeling composite amplification system comprises 6 STR loci on a human chromosome 15, 12 STR loci on a human chromosome 20 and corresponding 36 primer pairs, and each primer pair carries a fluorescent dye marker. The kit can be used as an effective supplement of an existing kit, the false positive rate can be remarkably reduced, the detection accuracy and reliability are effectively improved, an important basis is provided for prenatal diagnosis and genetic disease detection, and the kit has important clinical application value and wide market prospects.

The invention discloses a high-throughput composite STR (short tandem repeat) detection system based on next-generation sequencing. The high-throughput composite STR detection system comprises specific primer sequences designed for 291 STR loci; the 291 STR loci are composed of 106 autosome STR loci, one sex identification locus Amelogenin, 148 Y chromosome STR loci and 36 X chromosome STR loci, and the 291 STR loci are composed of 106 autosome STR loci, one sex identification locus Amelogenin, 148 Y chromosome STR loci and 36 X chromosome STR loci; the system is used for performing STR typing and is prepared into a kit for individual recognition, paternity identification and complex sample analysis. The STR detection system covers STR locus combinations of whole chromosomes (autosomes and X / Y chromosomes), breaks through the split design in the prior art, can overcome the limitation of the traditional capillary electrophoresis (CE) technology on complex samples, degraded samples and large-scale detection, improves the detection sensitivity, flux and accuracy, and has a good application prospect. And the requirements of identity identification of complex genetic relationships, mixed samples and disastrous events in judicial expertise are met.

The invention relates to a method for detecting trophoblast residues in an NK cell product and application of the method. The method comprises the following steps: extracting nucleic acid molecules of the NK cell product, and detecting whether trophoblast specific gene segments exist in the nucleic acid molecules or not. According to the method, the residual condition of the trophoblasts in the NK cell final product is detected by a molecular biological method, specifically, detection can be performed in a manner of amplifying specific genes of the trophoblasts through qPCR or amplifying STR loci of the NK cells through PCR, the method is simple, convenient and rapid, the accuracy rate is high, and the detection precision is improved by combining multiple methods, so that the accuracy of a detection result is ensured.

The present invention relates to the field of biotechnology, and relates to a multiplex amplification system, a kit and an application for 28 short tandem repeats. The multiplex amplification system includes 28 pairs of primers, which can simultaneously amplify 28 loci, and the primer sequences for the 28 loci are disclosed. Through the study of the genetic diversity of STR loci, the 28 loci selected by the present invention are located in the imprinted gene segments of the corresponding chromosomes. These loci have characteristics such as high individual discrimination power and high polymorphic information content, and can effectively and accurately detect the STR polymorphisms of the genome in a specific region. By comparing with the detection results of the parents, uniparental disomy in the corresponding segment can be indicated.

The invention belongs to the technical field of biological detection, and relates to a composite amplification kit for detecting X-STR gene loci, in particular to a composite amplification kit for simultaneously detecting 21 X-STR gene loci and application of the composite amplification kit in individual recognition and genetic relationship identification. Seven linkage groups are used in the detection kit, and the defect that in the prior art, the linkage relation of gene loci is not very clear, or few linkage groups are used, and the identification efficiency is low due to the fact that few linkage groups are recognized on X chromosomes is overcome. The detection kit provided by the invention can improve the identification efficiency and accuracy, is suitable for forensic individual identification and special genetic relationship identification, and has a good forensic application prospect.

This invention belongs to the field of genetics and provides a technique and data analysis method for simultaneously detecting 193 Y-STRs in the Chinese population based on an NGS platform. This invention discloses 193 Y-STR loci for human Y-chromosome STR genotyping; the selected 193 Y-STRs are used for library construction using a two-step PCR method. This invention has advantages such as rapid amplification, consistent efficiency, high sensitivity, and good data visualization; due to the high depth of high-throughput sequencing, it is more conducive to the analysis of mixed and degraded materials.

The present application relates to the technical field of sex chromosome STR identification, and particularly relates to an X chromosome STR locus composite amplification primer group, a 9-color fluorescent label composite amplification kit and application thereof.The composite amplification primer group comprises 45 pairs of specific amplification primer pairs, the 45 pairs of specific amplification primer pairs include 44 X chromosome STR loci and 1 pair of specific amplification primer pairs of a gender locus AMEL, and the nucleotide sequences of the primer pairs in the composite amplification primer group are shown as SEQ ID NO.1-90.The specificity of each primer in the composite amplification primer group is relatively strong, no primer dimer or interaction is generated during composite PCR amplification, the amplification efficiency difference of each pair of primers is relatively small, and the identification sensitivity is relatively high.The composite amplification primer group provides a molecular basis and core necessary reagent for simultaneously detecting 44 X-STR loci at a time.

The invention discloses a full-sib and half-sib relation identification supplementary composite amplification system and a kit. The composite amplification system comprises a plurality of STR (short tandem repeat) loci which are simultaneously amplified in a PCR (polymerase chain reaction) system. According to the multiple STR sites provided by the invention, the specific gene loci are 20 non-CODIS STR sites with high genetic polymorphism, 3 CODIS sites and 1 sex determination site glaze protein, and extremely high individual recognition rate and non-father exclusion rate are achieved by respectively designing primers and labeling fluorophores. The method can be used for forensic individual identification, paternity test and population genetics analysis, and is large in information amount and good in compatibility.

The invention discloses a kit for rapidly detecting 17 Y-STR gene loci of a micro-degraded sample and application of the kit. The kit comprises 15 pairs of specific amplification primer sequences for amplifying the 17 Y-STR gene loci. Compared with the prior art, the kit has the advantages that (1) the kit is combined with an eight-color fluorescence detection technology, the kit and a detection platform are deeply integrated, the detection process is optimized, and the detection speed and accuracy are improved; (2) according to the kit, the number of detectable sites in a 350bp range is greatly increased, so that the site detection number of detection materials such as conventional samples, old decayed samples, trace samples and degraded samples is greatly increased, and the purposes of forensic identification, genetic identification, family investigation and the like are achieved; and (3) in the forensic medicine application process, the kit has the advantages of high precision, high efficiency and high flux, so that the detection efficiency and accuracy of public security first-line cases are greatly improved.

The present invention discloses a detection kit, method and application thereof using a 10-color fluorescent autosomal STR composite amplification system. The detection kit uses nine fluorescent dyes, FAM, TET, HEX, ER650, ER652, ER663, ER664, ER674 and ER685, to label composite amplification primers of 48 STR loci, and uses fluorescent dye ER690 as an internal standard marker. The present invention breaks the limitations of the existing detection system's fluorescent channels and number and the number of loci, incorporates more mini STRs and STR sites with high polymorphism, and can obtain typing information of 48 STR loci through one amplification, thereby solving the problem of less detection site information in a single detection of trace samples, degraded samples, and difficult samples in complex environments, thereby effectively improving the detection efficiency of the system.

The present invention discloses a STR fluorescence-labeled multiplex amplification system, which includes 6 STR loci on human chromosome 15, 12 STR loci on human chromosome 20, and corresponding 36 primer pairs, and each primer pair carries a fluorescent dye label. The present invention can serve as an effective supplement to existing kits, can significantly reduce the false positive rate, effectively improve the accuracy and reliability of detection, provide an important basis for prenatal diagnosis and genetic disease detection, and has important clinical application value and broad market prospects.

The invention discloses a kit for pigeon individual recognition and paternity identification typing and application. The invention provides 16 novel STR loci for pigeon individual identification and paternity identification typing, and the STR loci are all tetranucleotide and pentanucleotide repeated loci. The kit established on the basis of the new STR site is clear, simple, convenient and accurate in typing result interpretation, the cumulative discriminability of the kit reaches 0.999999999999999999999999999999720944, and the combined exclusion probability of the kit reaches 0.9999999999720944. The individual identification capability is high, the genetic relationship identification is reliable, and a guarantee is provided for breeding of excellent racing pigeon varieties and genetic relationship identification.

The invention discloses a freeze-drying eight-color composite amplification kit with high inhibition resistance and application, and belongs to the field of molecular biology, a composite amplification system provided by the invention comprises specific primers for amplifying 31 gene loci, and the 31 gene loci comprise specific amplification primers of 29 STR gene loci, one sex assisting site Y indel and one Amelogenin site. The kit disclosed by the invention has the advantages of being rapid and efficient in detection of complex detection materials containing high inhibitors in on-site cases; all gene loci are compatible with an existing case database; and the time of case checking is shortened, the public security officers are helped to quickly lock the suspects, and the case cracking efficiency is improved.

The invention discloses a duck paternity test kit and an application thereof. The duck paternity test kit comprises amplification primers of gene loci of SICAU07-P, SICAU06-P, SICAU11-P and SICAU08-P. The marker for duck paternity test is selected from common STR loci, the loci have high genetic diversity and obvious genotype difference, high amplification efficiency and stability are shown in the PCR amplification process, the fluorescence labeling effect is good, and the accuracy of genotyping can be improved. Through fluorescence labeling, a plurality of STR sites can be simultaneously detected in the same reaction system, and the analysis flux is improved. In paternity test, Cervus software is used for analyzing the parent-child relationship between parents and offspring, the result shows that the sites can effectively distinguish the relationship between the parents and the offspring, the parent matching probability is obviously higher than that of non-parent pairing, and the high application value of the loci in paternity test is shown.

The invention provides a detection kit for detecting the genetic relationship of pigs and identifying individuals by using a fluorescent PCR capillary electrophoresis technology and application of the detection kit, and relates to the technical field of biology. According to the invention, STR loci published by ISAG and STR loci with high polymorphism obtained through high-throughput sequencing data analysis are combined and screened for the first time to obtain a group of STR loci suitable for pig genetic relationship and / or individual identification. STR loci are optimized to improve the specificity of the primers, no linkage effect exists between the STR loci and the primers, typing is rich, compatibility is good, and the detection result is accurate and reliable; the operation method is simple, and the detection time is short; a plurality of sites can be detected at one time, and the cost is low; the sensitivity is high, and accurate typing can be achieved when the DNA template amount is as low as 2 ng / mu L; the detection kit provided by the invention has good specificity and stability, no non-specificity is generated in multiple experiments, and the signal intensity is stable.

The invention discloses an SNP-STR (single nucleotide polymorphism-short tandem repeat) composite genetic marker detection kit and application thereof. The kit comprises specific primers for amplifying 21 STR gene loci, 12 SNP gene loci linked with the STR gene loci, a Y-STR gene locus, Y-Indel and Amel. The SNP-STR composite genetic marker has the advantages as follows: (1) the SNP-STR composite genetic marker meets the ANSI Standard standard for cell line identification, can be parallelly compared with data in an existing conventional mainstream database, and has good universality; (2) an SNP-STR composite amplification system is established and optimized, synchronous amplification of the two genetic markers is realized, the typing ability and cumulative individual recognition rate of a detection system are improved, and the method has important significance on identity identification and cross contamination identification of human cell lines; (3) a Y chromosome signal loss phenomenon caused by AMEL-Y gene deletion or mutation is overcome, and the accuracy of a human cell line sex determination result is improved; and (4) the kit is convenient and fast to operate and low in cost in the practical application process, and the detection speed and efficiency are greatly improved.

The invention provides a primer group, a kit, an amplification system and a freeze-drying material for simultaneously amplifying 25 human STR gene loci and a preparation method of the freeze-drying material. The primer group capable of simultaneously amplifying the 25 STR gene loci of the human comprises nucleotide sequences as shown in SEQ ID NO.1 to SEQ ID NO.50, and the primer group can simultaneously amplify and detect the 25 STR gene loci and realize efficient and specific detection on the 25 STR gene loci; meanwhile, the freeze-dried material for amplifying the 25 human STR loci is convenient to store and use, the amplification efficiency, the amplification balance and the amplification sensitivity of the freeze-dried material are still kept at the original level, and the use requirements of users are better met.

Novel Y-STR multiplex analysis designs, primer design, allelic ladders, methods of use and kits are disclosed, including the use of primer sets designed to provide amplicons for at least 11 Y-STR loci having a base pair size of less than about 220 bp, as well as the use of primer sets designed to provide amplicons for at least 22 Y-STR loci including at least 5 rapidly mutating loci.

The present invention discloses a composite amplification kit for simultaneously amplifying 20 horse STR loci and its application. The kit comprises specific amplification primers for amplifying the following 20 STR loci; wherein the 20 STR loci are: HTG6, HTG7, COR22, CA425, HTG4, COR82, LEX54, COR69, AHT5, HMS3, HMS1, HMS6, HMS7, COR58, HTG10, ASB17, VHL20, HMS2, ASB2, and LEX34. Compared with the prior art, the present invention has the following advantages: (1) the kit can simultaneously amplify and detect 20 horse STR loci in a single tube, making it the kit with the most detection loci among similar products; (2) the selected loci are distributed on different chromosomes as much as possible, and there is no genetic linkage between them, thereby improving the overall recognition ability of the kit; (3) the specific amplification primers do not interfere with each other, no non-specific peaks are generated, and the kit has the characteristics of strong specificity, high resolution, accurate typing, high sensitivity, etc., and can fully meet the various needs of horse paternity testing, individual identification, sex determination, etc.

The present invention relates to the field of dynamic mutation disease diagnosis, and specifically to a detection probe combination and sequencing analysis method for dynamic mutation STR loci. The present invention discloses a probe combination and detection method for detecting pathogenic STR loci and potential pathogenic STR loci. The present invention utilizes probe capture amplification technology in conjunction with third-generation long-read sequencing technology, and specifically designs calculation parameters for the number of STR sequence repeats, thereby achieving full coverage screening of pathogenic STR loci. Test results show that the screening results are accurate and reliable, with high detection efficiency. Multiple STR pathogenic loci can be screened simultaneously, improving the detection efficiency of STR loci and the clinical accessibility of large-scale screening. This overcomes the shortcomings of traditional detection technologies, such as a small number of target sites, high cost, and incomplete analysis, and provides convenience for the study of STR loci for dynamic mutation diseases. The method has significant clinical application value.