948 results about "Fluorescent labelling" patented technology

The invention provides a kit and a method for detecting common fusion genes of lymphoid leukemia based on a multiple digital PCR (Polymerase Chain Reaction) method. By constructing a synergistic effect mechanism of the primer and the fluorescent probe and optimizing matching of the primer, the probe and a fluorescent marker, non-specific binding among amplification products is effectively avoided. A composite reaction aid with a specific formula, namely a PCR enhancer, is innovatively introduced, so that the amplification efficiency and specificity of a multiple detection system are remarkably improved. According to the kit provided by the invention, the integrated detection capability on seven fluorescent channels, namely Atto 425, VIC, FAM, ROX, CY5, CY5.5 and CY7, is realized in a single-hole design. By innovatively constructing an ROX + CY5 and Atto425 + VIC dual-channel joint detection system, when the ROX and CY5 channels or Atto425 and VIC channels in the same detection hole simultaneously present positive signals, two additional detection targets can be specifically interpreted. The innovative detection strategy based on channel combination enables the kit to realize precise detection of 18 fusion genes only through two detection holes in a breakthrough manner.

The invention discloses an ion beam polishing method for coupled silicon carbide in-situ growth based on quantum dots, and belongs to the technical field of SiC surface polishing. The preparation method of the SiC material with the quantum dot sacrificial layer grown in situ comprises the following steps: mixing a lead source, a cesium source and a solvent to obtain a CsPbBr3 precursor solution; and dropwise adding the CsPbBr3 precursor solution to the surface of the SiC material, flattening, heating for crystallization, and annealing to obtain the SiC material with the quantum dot sacrificial layer grown in situ. According to the ion beam polishing method based on quantum dot coupling silicon carbide in-situ growth, the quantum dot layer serves as the sacrificial layer of the SiC wafer, the thickness of the sacrificial layer can be effectively reduced, the flatness of the SiC surface can be remarkably improved, and the good fluorescence labeling effect can be achieved. The CsPbBr3 quantum dot prepared by the method has extremely small nano size, can deeply fill micro cracks and micro-nano scratches on the surface of a SiC wafer, is completely coupled with the micro cracks on the surface of the SiC wafer, and realizes accurate filling of defects.

The invention discloses a radiotherapy beam output control method, system, device and equipment and a medium, and relates to the technical field of radiotherapy control, and the method comprises the steps: obtaining the spatial position information of a patient body surface marking module in real time; wherein the marking module comprises a fluorescent marking ball, and the fluorescent marking ball is fixed in a breathing movement remarkable area of a patient; performing information conversion processing on the spatial position information according to a time axis direction to obtain target breathing state change information of the patient, and importing the target breathing state change information into a corresponding preset breathing standard normal form to check whether the target breathing state change information meets a radiotherapy treatment condition; and if the target breathing state change information meets the radiotherapy treatment condition, recommending a corresponding breathing gating window threshold value, and dynamically controlling the output of the radiotherapy beam according to the breathing gating window threshold value. The automatic monitoring of the breathing state change is realized, and the output of the radiotherapy beam is accurately controlled according to the monitoring result.

The invention discloses application of iron ruthenium nano-enzyme in preparation of nucleic acid detection products, and belongs to the technical field of biological detection and nano-materials. The RIN has stable peroxidase-like (POD) activity, and can catalyze a chromogenic reaction between 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) and hydrogen peroxide (H2O2). The catalytic activity of the nano-enzyme can be selectively inhibited by pyrophosphate (PPi) generated in a nucleic acid amplification reaction, so that visual colorimetric analysis on whether target nucleic acid exists or not is realized. In combination with a polymerase chain reaction (PCR) system, the invention constructs a new nucleic acid detection strategy which does not need fluorescence labeling, is simple and convenient to operate and has high sensitivity. Through verification, the system can be widely applied to rapid on-site screening and visual interpretation of related nucleic acid of drug-resistant pathogens (such as ESKAPE flora) and the like, and has a good application prospect.

The invention provides a liquid leakage detection method and device for a liquid cooling device, and the method comprises the steps: obtaining the fluorescence intensity and magnetic field gradient of a surface detection region of the liquid cooling device; wherein the cooling liquid of the liquid cooling device contains a fluorescent marker and a magnetic marker; based on the fluorescence intensity and the magnetic field gradient, determining whether a liquid leakage risk exists in the detection area; in response to the liquid leakage risk of the detection area, obtaining the liquid leakage probability of each grid position of the detection area based on the fluorescence intensity and the magnetic field gradient; and determining a target grid position where liquid leakage occurs in the detection area based on the liquid leakage probability. According to the technical scheme of the invention, the detection precision and detection efficiency of liquid leakage of the liquid cooling device can be improved.

The invention discloses a reagent for rapidly distinguishing bacterial and virus infection and application, belongs to the field of molecular biology, relates to medical and biotechnology, and aims to solve the problem that a traditional method cannot clearly distinguish bacterial infection from virus infection. The reagent comprises five antibodies marked with different fluorescence. The five antibodies are all mouse anti-human or rabbit anti-human antibodies, and targets corresponding to the five antibodies are respectively CD45, CD14, CD64, CD169 and HLA-DR (human leukocyte antigen-DR). The tail end of the antibody is connected with different fluorescein through chemical reaction; fluorescence labeling is performed on neutrophils, mononuclear cells and lymphocytes in peripheral blood through an antibody combination of CD molecules, and corresponding infection indexes are calculated through a flow cytometry method, so that the infection type (bacterial / virus infection) of a patient is quickly distinguished and judged.

The invention discloses a visual detection test method for damage cracks of a solidified soil test piece, and particularly relates to the technical field of geotechnical engineering material detection. The method comprises the following steps: doping fluorescence labeling particles in a solidified soil test piece, and applying periodic load to induce crack formation after curing and forming; synchronously exciting fluorescent particles through an ultraviolet excitation light source, and collecting a short-wave fluorescence response image sequence; inputting the image sequence into a deep residual neural network model, identifying a nonlinear enhancement region of a fluorescence signal, and deducing a crack initiation position and a propagation path; constructing a three-dimensional dynamic model of crack evolution in combination with an image space reconstruction algorithm and time sequence comparison, extracting indexes such as a crack propagation rate, a bending angle and crack density, performing comparative analysis on the indexes and initial parameters of a material, evaluating a microstructure damage development mechanism, and outputting a visual damage evolution result; the method provided by the invention can realize high-precision, non-destructive and dynamic detection of the fine cracks in the solidified soil, and has the advantages of high identification sensitivity, strong modeling precision and good engineering adaptability.

The invention discloses an ink-uniformizing jet printing process for a battery UV insulating coating, which comprises the following steps: adding a first fluorescent marker and a second fluorescent marker in a UV coating material, the two fluorescent markers being capable of performing fluorescence resonance energy transfer to obtain a marked UV coating material; carrying out multi-position spectral characteristic acquisition on the micro-droplets formed by the UV coating material; dynamically tracking the spreading process of the micro-droplets; exciting the coating in the curing process by adopting multi-wavelength exciting light to obtain cross-linking degree distribution data; and carrying out thermoacoustic scanning on the coating. According to the technical scheme, real-time monitoring of cross-linking degree distribution in the curing process of the UV insulating coating can be achieved, the problem of non-uniform cross-linking of the coating can be found in time by monitoring the whole process of micro-droplet formation, spreading and curing in combination with fluorescence spectrum analysis and thermoacoustic detection technologies, and the quality of the coating is improved. And an effective quality control means is provided for ensuring the long-term reliability of the battery insulating coating.

The invention provides a multi-modal image fusion endoscope operation target positioning device and method, and the method comprises the steps: calibrating internal and external parameters of a binocular camera and a fluorescence camera, and obtaining a pose conversion matrix between the fluorescence camera and the binocular camera; using a U-Net network model to identify and track the position of the fluorescent mark point in the fluorescent image in real time, and obtaining the two-dimensional image coordinate of the fluorescent mark point; processing a left image and a right image of the binocular camera by using a region-based local stereo matching method in binocular stereo imaging to obtain a point cloud image of a target space; and obtaining three-dimensional pose information of the fluorescent mark point by combining the two-dimensional image coordinate of the fluorescent mark point and the point cloud image of the target space. The binocular stereoscopic vision and the fluorescence imaging technology are combined, the three-dimensional pose and depth information of the fluorescence mark point is obtained by registering the information between the fluorescence camera and the binocular camera, more accurate and real-time vision assistance is provided for an operation, and the safety and efficiency of the operation are improved.

The invention discloses an ANA detection image-text report intelligent generation method and system, and relates to the technical field of immunodetection, the system uses different fluorescent markers to mark ANA, signal sensitivity is enhanced, and detection accuracy is ensured. Then, collecting fluorescence signals of different wavelengths by adopting a fluorescence microscope and a multi-wavelength excitation light source, and obtaining dynamic change data of reaction intensity by combining a time-resolved fluorescence imaging technology to generate a multi-channel imaging data set; thirdly, noise removal, signal enhancement and image alignment are carried out through an image preprocessing technology, and data consistency is ensured; based on a multi-channel data set, a convolutional neural network is used for training and testing an initial model, an intelligent detection model is constructed, a fluorescence reaction mode is extracted, finally, a detailed ANA detection report is automatically generated by calculating a comprehensive scoring coefficient and comparing the comprehensive scoring coefficient with a preset threshold value, and an accurate immunodiagnosis basis is provided for doctors.

The invention relates to a method and a system for simultaneously acquiring complete form and omics molecular information of a single neuron. The method comprises the following steps: acquiring fluorescently-labeled samples of cell bodies, dendrites and axons of the neuron; performing cyclic operation of scanning imaging and cutting on the section of the sample until the scanning imaging of the whole sample is completed, and collecting a section containing a fluorescence-labeled neuron cell body obtained by cutting; the method comprises the following steps: reconstructing a sample section three-dimensional image obtained based on scanning imaging operation to obtain a complete form of neurons, extracting fluorescently-labeled neuronal cell bodies from collected slices, and obtaining omics molecular information of the neurons through omics analysis. According to the method, the fine and complete form and omics molecular information of the same neuron can be effectively integrated, and the problem that the two kinds of key information cannot be obtained at the same time at the single neuron precision level is successfully solved.

The invention discloses an intelligent visual detection system for a high-precision printed circuit board, and relates to the technical field of precision optical detection.The intelligent visual detection system comprises the steps that based on a printed circuit board substrate with a quantum dot mark layer attached to the surface, confocal scanning is conducted through laser wavelength, and an original image data set is generated; performing multispectral feature analysis on the original image data set, capturing focus offset rules of light rays with different wavelengths, and generating a wavelength-focus mapping relation compensation instruction set; according to the compensation instruction set of the wavelength-focus mapping relation, an imaging light path is adjusted in real time, and a high-fidelity fusion image is generated; and analyzing optical signal feature changes in the high-fidelity fusion image, identifying an abnormal region, extracting microdefect features, and generating a detection report containing the defect features. According to the invention, through uniform dispersion of the quantum dot modified ink in the inert atmosphere and a plasma substrate activation process, molecular-level combination of the solder mask layer and the fluorescent marking material is realized.

A fluorescence intensity enhancer containing a water-soluble compound having a melting point of 50 DEG C or higher, a method for enhancing the fluorescence intensity of a fluorescently labeled target biological substance using the fluorescence intensity enhancer, and a fluorescence detection kit containing the fluorescence intensity enhancer.

The invention belongs to the technical field of synthetic chemistry, and provides a muscarinic acetylcholine receptor 3 fluorescent probe as well as a preparation method and application thereof. The chemical structural formula of the fluorescent probe is shown in the specification, and the fluorescent probe can be used for M3 receptor tracing in cells or organisms. The probe provided by the invention has a good fluorescent property and can be influenced by the polarity and viscosity of an external environment; and the probe has good biocompatibility, and the IC50 of the probe is 43 times of the concentration of a working solution, so that the probe can meet the requirements of cell fluorescence imaging. In the aspect of cell fluorescence imaging, the probe can realize selective fluorescence labeling of the M3 receptor in the cell, washing is not needed in the dyeing process, and the operation is simple and convenient, so that visual research on positioning of the M3 receptor in the cell is realized.

The invention discloses a preparation method of an RNA targeted photosensitizer and an application of the RNA targeted photosensitizer in anti-tumor treatment, and belongs to the technical field of fine chemical engineering. The photosensitizer is modified on the basis of a fluorescent molecule parent, and the RNA targeted photosensitizer is constructed. The photosensitizer disclosed by the invention can be self-assembled into nanoparticles in an aqueous solution, shows good RNA targeting, effectively generates reactive oxygen species through I-type and II-type mechanisms, and shows excellent performance in the aspects of long-term fluorescence imaging in tumor cells and anti-tumor treatment in normal-oxygen and hypoxic environments; the fluorescence of the photosensitizer Se-cy is enhanced by about 230 times after RNA is added, and the fluorescence duration can be kept as long as 40 hours in in-vitro cells; and the fluorescent label can be kept for 4 days in vivo, shows excellent fluorescent durability, and has a remarkable anti-tumor effect on tumor-bearing mice.

The invention provides a disease marker epitope prediction and antibody screening method, which belongs to the technical field of disease markers, and comprises the following steps: firstly, establishing a training data set containing a known antigen-antibody compound structure and disease tissue expression data; and obtaining target protein sequence information through liquid chromatography-mass spectrometry analysis and carrying out sequence comparison. And then a deep convolutional neural network is utilized to extract sequence features, and surface exposure sites are identified by combining secondary structure prediction and solvent accessibility analysis. After the features are integrated with sequence evolution conservative properties, a prediction model is constructed by using a random forest classifier. The method comprises the following steps: carrying out molecular dynamics simulation on a prediction result, screening first 10% of candidate sequences through a comprehensive scoring function and K-means clustering, and finally determining an antigen epitope sequence with the strongest binding activity through verification of an antigen chip and a fluorescence labeled antibody system, so that the technical problem that specific antigen epitopes are difficult to accurately predict and recognize in the prior art is solved.

The invention provides a carbide evolution real-time monitoring method and device, a terminal and a storage medium, and relates to the technical field of material deformation measurement. The method comprises the following steps: acquiring a plurality of images at different stretching stages in the in-situ stretching process of a metal sample; wherein the metal sample is subjected to surface modification and fluorescence labeling; identifying a fluorescence intensity gradient in each image; and converting the fluorescence intensity gradient of each image into a stress value to obtain stress distribution of the surface of the metal sample in the image, and obtaining a carbide evolution monitoring result of the metal sample based on the stress distribution of the surface of the metal sample in each image. According to the invention, the real-time visualization of the carbide evolution process and the influence on the matrix and mechanical properties can be realized.

The invention provides a primer group for identifying multiple genes of ten bacteria related to lower respiratory tract infection and application of the primer group, and belongs to the technical field of gene detection, the sequence of the primer group is shown as SEQ ID NO.1-20, the primer group designs specific primers for ten bacteria such as pseudomonas aeruginosa and streptococcus pyogenes, and the specific primers of ten genes of a 5'end primer are labeled by FAM fluorescence. 10 target gene loci and 2 internal reference loci can be detected in one PCR system, the traditional PCR deviation is overcome, and gene expression is accurately quantified. The reagent and the kit developed on the basis have the advantages of simplicity and convenience in operation, low cost, high accuracy and the like. When the kit is used, DNA of a sample is extracted for multiple PCR, and pathogen load is determined through electrophoresis and software analysis. Experiments prove that the method has strong specificity and high sensitivity to 10 bacteria, and has important application value in LRTI bacterial identification.

The invention discloses an STR (short tandem repeat) fluorescence labeling composite amplification system, the STR fluorescence labeling composite amplification system comprises 6 STR loci on a human chromosome 15, 12 STR loci on a human chromosome 20 and corresponding 36 primer pairs, and each primer pair carries a fluorescent dye marker. The kit can be used as an effective supplement of an existing kit, the false positive rate can be remarkably reduced, the detection accuracy and reliability are effectively improved, an important basis is provided for prenatal diagnosis and genetic disease detection, and the kit has important clinical application value and wide market prospects.

The present invention relates to pathogen cleaning and simulation of pathogen spreading. More particularly, some implementations of the described invention relate to systems and methods for spraying materials in a manner that mimics the spreading of pathogens from coughing or sneezing. Some implementations further relate to using a non-aerosol sprayer that produces both a mist and droplets of a mixture containing water, a fluorescent marker, a surfactant, and an antifoaming agent. In some cases, the pump sprayer defines an internal compartment that includes the mixture. In some cases, the sprayer further includes a pump mechanism that is configured to be pumped to compress a gas within the compartment and a release valve that is configured to release pressure within the compartment through a spray nozzle of the sprayer such that droplets and mist of the mixture are sprayed from the nozzle. Other implementations are also described.

The present invention discloses the use of extracellular vesicles derived from oligodendrocytes carrying FTH1 in differentiating between multiple system atrophy and Parkinson's disease, belonging to the field of biomedical technology. The present invention utilizes fluorescently labeled CNPase antibodies and FTH1 antibodies to accurately detect extracellular vesicles derived from oligodendrocytes carrying FTH1 in plasma. By detecting the concentration of extracellular vesicles derived from oligodendrocytes carrying FTH1 (FTH1-positive) in plasma, it is possible to accurately distinguish between MSA patients, PD patients, and healthy individuals. This provides a highly effective biomarker for MSA diagnosis, improves the ability to diagnose MSA early and differentiate between MSA and PD, and effectively addresses the complexity and low sensitivity issues faced by traditional diagnostic methods.

The present disclosure relates to proteomics, including the detection of immune cell target proteins as examples that the disclosed methods can be used for protein detection. Methods, compositions, and systems are described for identifying the presence of proteins, including detecting immune cell proteins from a sample using a combination of multiplex immuno-PCR with antibodies conjugated to oligonucleotides comprising an antibody-specific barcode flanked by PCR priming sites; detection of barcoded amplicons obtained from immuno-PCR with encoded padlock probes comprising codes specific for the barcodes / target protein; rolling circle amplification (RCA) of the circularized padlock probes; and determination of the sequence of the codes amplified by RCA by next generation sequencing or using fluorescently labeled hybridization probes.

The present disclosure relates to methods for determining concentration of a lysine analog antifibrinolytic agent in a sample. Also provided are kits comprising (a) a composition comprising a fluorescently labeled inactive plasminogen variant and a fluorescence quencher labeled fibrin-degradation product (FDP) and (b) a lysine analog antifibrinolytic agent standard and methods of use thereof.

The invention provides a miss-seeding detection and reseeding method and a miss-seeding detection and reseeding system for a potato punching seeder. According to the method, a three-dimensional motion track is collected through an inertial navigation device, and a seeding path topological graph is generated in combination with a mechanical trigger signal; spraying a fluorescence labeling substance to the surface of the soil at the tail part of the seeding machine, and marking a topological graph coordinate corresponding to the spraying moment as a miss-seeding risk area; capturing furrow track flight by using a spectrum, and mapping the image coordinates of the mark points to the coordinates of the miss-seeding risk area in the topological graph by identifying the spectral response signal of the fluorescence mark; generating a reseeding operation path curve based on the aggregation form of the miss-seeding risk area coordinates and the furrow extension direction; and driving the reseeding device to move along the curve, and when the positioning coordinate of the reseeding device coincides with the coordinate of the miss-seeding risk area, activating the seeding mechanism to execute single-grain reseeding and updating the state identifier. According to the application, the potato seeding leak detection and reseeding efficiency and the reseeding position accuracy are improved.

The invention discloses a zebra fish spermatogonial stem cell separation and transplantation method, and belongs to the technical field of fish germline stem cell separation. The method comprises the following steps: washing complete sexual glands of zebra fish testis by adopting penicillin / streptomycin double-antibody-containing PBS (Phosphate Buffer Solution), physically shearing, digesting and dissociating, and filtering by a screen, so as to obtain zebra fish testis cell suspension; performing density gradient centrifugation to obtain zebra fish spermatogonial stem cells; dyeing, marking, centrifuging, washing, re-suspending, and carrying out micro-injection transplantation to enter the back of the abdominal cavity of the acceptor zebrafish juvenile fish. According to different physical properties of different cells, spermatogonial stem cells are separated, purified and transplanted from zebra fish testis cell suspension by adopting a density gradient centrifugation method, and migration and proliferation processes of donor germ cells can be observed in receptor fish after fluorescence labeling. And a new technical path is provided for fish germplasm resource preservation, endangered fish protection, economic cultured fish yield increase and new variety cultivation.

Disclosed are three-dimensional image registration via dense staining and a super-resolution multiplexed imaging method using the same. The three-dimensional image registration includes: placing at least one fiducial marker in an internal structure of a biological sample via dense staining; obtaining a plurality of images by repeatedly imaging the biological sample while replacing a fluorescent label attached to at least one target molecule within the biological sample where the fiducial marker is placed; and registering the images using the fiducial marker as a reference to obtain a final image of the target molecule.

The invention provides a primer / probe composition for capillary electrophoresis analysis, a detection method and application. The primer / probe composition comprises a forward primer, a reverse primer and a nucleic acid probe, the 5'end of the nucleic acid probe carries a fluorescent label, and both the forward primer and the reverse primer do not carry the fluorescent label. Different targets are identified by detecting the nucleic acid probe, so that the length of an amplification product is not limited, only the nucleic acid probe carries a fluorescence signal without generating a non-specific fluorescence signal, the generation of a supersaturated fluorescence signal can be effectively prevented by using the nucleic acid probe with a specific concentration, the fluorescence signals among different targets do not interfere with each other, and the detection sensitivity is high. And multicolor fluorescence can be used at the same time.

The invention relates to the technical field of biomedicine detection reagent manufacturing and reproductive medicine, in particular to an embryonic development potential sperm key protein kit and a use method thereof. The method is characterized in that an antibody combination capable of specifically recognizing a group of key proteins related to embryonic development potential in human sperms is prepared, and the antibodies, a fluorescence labeled secondary antibody, a cell nucleus dye, a concentrated washing liquid, a mounting medium and a pre-coated control porous glass slide are assembled into the special detection kit. The kit can be combined with a multi-mode imaging system (such as a fluorescence / confocal microscope and a common optical microscope) and image analysis software to realize the traditional analysis of sperm morphology and movement ability, and can perform synchronous and accurate qualitative and quantitative analysis with the expression level and subcellular localization of the key protein. The invention provides an integrated molecular detection solution, and provides a powerful tool for sperm quality screening and embryonic development potential prediction in an assisted reproduction technology.

The invention discloses a nanoparticle scattered light confocal imaging device and method. The device comprises a laser unit, a first pinhole, a beam splitter turntable, a scanning focusing unit, an electric objective table, a first detection imaging unit, a second pinhole, a light splitting unit and a second detection imaging unit. The method does not need to mark nano particles, and can synchronously collect unmarked nano particles and fluorescence-labeled biomolecules; in-situ imaging can be realized, and the distribution position and content of nano-particles in living cells can be directly observed; the imaging resolution ratio is high, single nanoparticle imaging can be carried out, and fluorescence imaging can also be carried out at the biomolecule level.

The utility model provides a micro-fluidic chip for unmarked or fluorescently marked single cell sorting and a single cell sorting system, which do not adopt means for damaging a sorting target, such as high voltage, strong electric field, high-voltage sheath fluid and the like, and can be used for sorting the unmarked or fluorescently marked single cells on the premise of keeping activity. For unlabeled or labeled (such as fluorescence labeled) cells, bacteria, proteins, viruses, genes (fragments or whole segments), cell clusters and other microorganisms, or liquid drops, liquid segments, bubbles or blocky solids containing the biological particles, high-efficiency, accurate and reliable single-individual detection, control and sorting (especially including but not limited to single-cell sorting) are carried out.