1538results about "Nucleotide libraries" patented technology

The disclosure provides systems and methods for droplet processing. For example, a method can include providing a first droplet and a second droplet. In some cases, the first droplet may have a first concentration of a reagent and the second droplet may have a second concentration of the reagent. The second droplet may comprise a bead or a biological particle. The method can also include subjecting the first droplet and the second droplet to conditions sufficient to transfer the reagent from the first droplet to the second droplet, thereby decreasing the first concentration in the first droplet and increasing the second concentration in the second droplet.

The invention provides a large yellow croaker medium-low density SNP liquid phase chip and application thereof, the chip is prepared from a probe for detecting 15,292 SNP molecular marker combinations of a large yellow croaker whole genome, and the position and base information of each SNP site on the chip are shown in a table 1. The invention further provides a preparation method and application of the chip, such as application in large yellow croaker population genetic diversity analysis, breeding population genotype identification, character correlation analysis or genome selective breeding. The chip designed by the invention comprises a series of core sites obviously related to growth, flow resistance and quality characters, and also comprises a large number of background sites, so that the detection accuracy of the designed chip is guaranteed, and the chip has the obvious advantage of low detection cost, the detection cost is reduced by more than 30%, and the detection efficiency is improved. The scientific and technological innovation of the large yellow croaker dark blue breeding industry can be further accelerated.

The invention discloses a cowpea 5K SNP (Single Nucleotide Polymorphism) liquid chip and application thereof in variety identification, and belongs to the technical field of biology. The liquid chip provided by the invention comprises a set of nucleotide probes for detecting 5847 SNP sites of cowpea, and can be widely applied to different scenes of germplasm resource identification, genetic diversity evaluation, variety identification, genetic relationship identification, purification and rejuvenation of local varieties and the like of different cowpea varieties; an effective scientific basis is provided for cowpea variety identification, the variety identification and evaluation efficiency is improved, and the method has a remarkable application value.

A method of making a barcoded surface includes synthesizing a plurality of arrays arranged in rows and columns. Each array can include a plurality of discrete reaction sites with a first oligonucleotide containing a first unique array barcode sequence. The first unique array barcode sequences of the first oligonucleotides corresponds to a mapped discrete reaction site location within the array. A second oligonucleotide can be coupled to the first oligonucleotide that contains a second unique barcode sequence. The first unique barcode sequences of the second oligonucleotides corresponds to a mapped first sub-group location within the plurality of arrays.

The invention discloses a soybean SNP molecular marker combination, a chip and application thereof, and relates to the field of soybean molecular genetic breeding. The SNP molecular marker combination developed by the invention is uniformly distributed on 20 chromosomes of a soybean whole genome, and the accuracy of related research application such as gene positioning can be improved. The SNP molecular marker combination provided by the invention is rich in information amount and has important value in variety identification, correlation analysis and molecular marker-assisted breeding. On the basis, detection products such as SNP typing chips, kits and the like which are high in capture rate, high in density and high in polymorphism and are uniformly distributed in a genome can be developed.

The invention belongs to the field of molecular biological identification, and particularly relates to a specific SNP (Single Nucleotide Polymorphism) site combination for identifying a Wuzhishan pig variety and application. The specific SNP site combination for identifying the Wuzhishan pig variety comprises 77 SNP sites, and the physical positions of the SNP sites are determined by sequence comparison based on a pig reference genome Sscrofa11.1. The specific SNP site combination is screened based on a method of combining whole genome association analysis with selection signal analysis so as to ensure the accuracy of site selection. The selected specific SNP site combination can rapidly realize accurate identification of the Wuzhishan pig variety on the gene level, and has significant application value in the aspects of genetic resource accurate protection and variety utilization of Hainan Wuzhishan pigs.

The invention discloses a sheep whole genome SNP molecular marker, which comprises 50000 SNP molecular markers, the SNP molecular marker is composed of 5235 functional SNP molecular markers and 44765 SNP molecular markers, the functional SNP molecular markers mainly select variation sites related to economic traits (such as growth, slaughtering, meat quality, breeding, lamb and the like) of sheep, and the variation sites are selected from the variation sites. And the background SNP molecular marker is used for improving the typing precision and polymorphism coverage of the chip, so that the applicability of the chip in a large-scale group is ensured.

A high-throughput screening method for an aptamer, and a use. An implementation route is divided into three parts, a first part is construction of a library of a pre-enriched aptamer and sequencing same, a second part is generation of an aptamer cluster on a slide, and a third part is combination of a target protein and the aptamer cluster. Provided is a method for high-throughput rapid detection of an aptamer. The method has the beneficial effects: sequencing time, sequence synthesis and affinity re-verification time are saved, the affinity verification costs are greatly saved, rapid screening of aptamers can be promoted, and the actual use of aptamers is promoted along with the increase in the types and numbers of aptamers. The existing platform BGISEQ 500 of Beijing Genomics Institute can be used to grow an aptamer cluster on a chip by using DNB as a template, and the use of sequencers is expanded by integrating sequencing, sequence synthesis and affinity identification.

Disclosed are a genome-wide low-density SNP chip for milk goats and a use thereof. The provided SNP chip is applicable to genetic improvement of economic traits of European milk goat varieties and Chinese native milk goat varieties, can also be applied to a plurality of fields of milk goat genome-wide association study, genome-wide selection breeding, population genetics analysis, trait-related candidate genes, QTL analysis and locating, and the like, and has high value in production application and scientific research.

The invention belongs to the technical field of whole-genome gene chips, and particularly relates to a leymus chinensis whole-genome 50K liquid chip and application thereof. According to the leymus chinensis whole genome 50K liquid phase chip, a whole genome re-sequencing technology is utilized, an SNP molecular marker combination of leymus chinensis is determined, and then the leymus chinensis whole genome 50K liquid phase chip is developed. The chip is combined with a high-density SNP probe, realizes high-throughput and high-precision genetic typing of leymus chinensis genomes, and particularly has wide application prospects in the field of molecular breeding.

The invention discloses a whole-genome low-density chip for breeding high-yield and long-life characters of dairy cows and application of the whole-genome low-density chip. The invention firstly discloses a whole-genome low-density chip for breeding the high-yield and long-life character of the dairy cow, the whole-genome low-density chip comprises 10000 SNP (Single Nucleotide Polymorphism) loci, ARS-UCD1.2 is used as a reference genome, and the information of the 10000 SNP loci is shown in a table 6. The invention further discloses application of the whole-genome low-density chip. The 10,000 SNP loci contained in the whole genome low-density chip are uniformly distributed on chromosomes, cover the whole genome and important character loci, can effectively improve the longevity and the accuracy of milk yield breeding, and can be applied to gene screening, gene positioning, marker-assisted breeding and the like. In addition, the detection cost of the low-density chip is greatly reduced, and the low-density chip is more suitable for commercialized dairy farms and has important significance on dairy cow breeding and dairy cow group improvement.

A dual screen technique using DEL and 2DCS screens with libraries of synthesized small molecules and synthesized RNA structures (4,096 targets) delivers bona fide ligand-RNA 3D fold target pairs. One of the newly discovered ligands bound a 5′GAG / 3′CCC internal loop that is present in pri-miR-27a, the oncogenic precursor of microRNA-27a. The DEL-derived pri-miR-27a ligand is cell-active, potently and selectively inhibits pri-miR-27a processing to reprogram gene expression and halt an otherwise invasive phenotype in triple-negative breast cancer cells.

Systems and methods are provided for determining an optimized probe set. The method proceeds by obtaining a set of probes, where each probe has a respective concentration. The set of probes is assayed against a sample library, and at least i) a respective recovery rate for each probe in the set of probes, and ii) a median recovery rate for the set of probes are obtained. Modify the respective concentration of each probe that does not satisfy predetermined recovery rate threshold. Reevaluate the set of probes against the sample library. Repeat the modifying and reevaluation until the respective updated recovery rate for each probe in the updated set of probes satisfies the predetermined recovery rate threshold, thereby providing the optimized set of probes for the sample library.

The present disclosure provides virus-like particles (VLPs) for delivering prime editors, and systems comprising such prime editor (PE) VLPs. The present disclosure also provides polynucleotides encoding the PE-VLPs described herein, which may be useful for producing said PE-VLPs. Also provided herein are methods for editing the genome of a target cell by introducing the presently described PE-VLPs into the target cell. The present disclosure also provides fusion proteins that make up a component of the PE-VLPs described herein, as well as polynucleotides, vectors, cells, and kits.

Provided are a dairy goat whole-genome 55K SNP chip and a use thereof. The chip has a total of 54344 SNP sites. The provided dairy goat whole-genome 55K SNP chip can be used for the identification and kinship analysis of imported dairy goat breeds and domestically bred dairy goat breeds, and can also be used in multiple fields such as dairy goat whole-genome association analysis, whole-genome selective breeding, population genetics analysis, and trait-related candidate gene and QTL site analysis and positioning, and has high value in both production applications and scientific research.

The invention discloses a cucumber whole genome SNP (Single Nucleotide Polymorphism) site combination, a liquid chip and application of the cucumber whole genome SNP site combination. On the basis of whole genome re-sequencing data and GBS data of cucumbers, a group of SNP locus combinations and liquid phase chips which are uniformly distributed in the whole genome are developed, 17519 SNP loci are selected according to the whole genome re-sequencing data, 7340 SNP loci are selected according to the GBS data, and finally 24859 SNP loci are selected. 24859 SNP loci are uniformly distributed on the whole genome of the cucumber, so that an efficient whole genome genotyping tool is provided for genetic diversity analysis, variety identification, background screening, molecular marker-assisted breeding, gene positioning and whole genome association analysis of the cucumber, and the related research process of the cucumber is promoted. The method has an important application value.

The invention belongs to the field of preparation of medical intermediates, and particularly relates to a preparation method of an On-DNA sulfinic acid structure compound. The method for preparing the On-DNA sulfinic acid structure compound comprises a step of oxidizing On-DNA thiol (phenol) into the On-DNA sulfinic acid structure compound under alkaline and heating or illumination conditions, a solvent used in the reaction step is a mixed solution of water, dimethyl sulfoxide, N, N-dimethylformamide, acetonitrile, ethanol or tetrahydrofuran, the reaction temperature is not more than 80 DEG C, and the reaction time is not more than 2 hours. And the illumination wavelength ranges from 365 nanometers to 535 nanometers. The On-DNA sulfinic acid structure compound obtained in the invention can be directly used for constructing a structure compound with a sulfonyl group, the reaction condition is mild and convenient, a new method and a new idea are provided for construction of a DNA coding compound library, and the On-DNA sulfinic acid structure compound has a very good application prospect in lead drug development.

The embodiment of the invention discloses an SNP chip of channa maculata and application. The SNP chip comprises a probe combination which is fixedly provided with markers respectively aiming at SNP1-SNP50000. The chip has the advantages of uniform site distribution, high typing accuracy, strong repeatability and higher flexibility, is suitable for mainstream next-generation sequencing platforms such as Illumina and MGI, and has universality. A reliable genotyping technology can be provided for correlation researches such as population genetics analysis, genetic relationship identification, whole genome correlation analysis and genome selection of channa maculata, the breeding efficiency of channa maculata is improved, and the development of the aquatic product breeding industry is promoted.

The invention discloses a specific molecular identity card for identifying a pier-head pig and application of the specific molecular identity card. The specific molecular identity card is a unique homozygous SNP (Single Nucleotide Polymorphism) site set of the pier-head pig. According to the method, the specific molecular marker of the pier-head pig is screened at the genome level through a molecular biological technology, and the symbolic genetic difference between the pier-head pig and other varieties can be revealed, so that the purpose of identifying the varieties is achieved. The method for obtaining the specific molecular marker of the pier head pig comprises the following steps: carrying out selective clearance analysis by calculating a genetic differentiation index (Fst) and a nucleotide polymorphism (pi) ratio, screening a selected region of the pier head pig, selecting a homozygous SNP site of the pier head pig in the region as candidate specific SNPs, carrying out genetic typing by virtue of a KASP typing technology, and screening out the specific molecular marker of the pier head pig. And counting the genotype frequency of each site in different varieties so as to screen out the specific molecular marker of the pier-head pig.

The use of an SNP site combination of Litopenaeus vannamei in trait evaluation of mixed-family culture, a probe and a kit. The SNP site combination of Litopenaeus vannamei comprises 1125 SNP sites, wherein the physical positions are determined on the basis of alignment with a reference genome sequence of Litopenaeus vannamei. Also disclosed are a molecular probe for capturing the SNP site combination and a kit comprising the molecular probe. By means of using the SNP site combination for paternity identification, the present invention can achieve the mixed culture of individuals of different families in the early stage, so that the common environmental effect caused by separate family culture in the early stage is effectively reduced, and the accuracy of a trait evaluation result of mixed culture is improved; moreover, the genetic relationship between individuals can be accurately calculated. Compared with physical marks, the present invention avoids the phenomena of falling and dislocation of marks on individuals and human identification errors, thus obtaining accurate pedigrees.

To provide a biochip and a manufacturing method in which the problem of overflow of a liquid specimen is avoided, and which can deal with a large amount of the liquid specimen.SOLUTION: A biochip comprises a substrate 110, an insulator layer IL, a semiconductor layer 120, a dielectric layer 130, a metal layer 140, and a protective layer 150. The semiconductor layer is disposed on the insulator layer, and has a reaction zone 121. The dielectric layer is disposed on the semiconductor layer, and has a first aperture O1. The metal layer is disposed on the dielectric layer, and has a source, a drain and a wall structure. The wall structure 141 surrounds the first aperture, the source and the drain. The protective layer is disposed on the metal layer, and has a flat part 151, a protruding part 152, a second aperture O2, and a third aperture O3. The flat part surrounds and defines the second aperture. The protruding part is disposed to correspond with the wall structure. The protruding part surrounds and defines the third aperture. The second aperture connects the third aperture and the first aperture, and allows the reaction zone to be exposed.SELECTED DRAWING: Figure 6

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker combination, a molecular probe combination, a liquid phase chip and a kit for corn genotyping and application of the SNP molecular marker combination, the molecular probe combination, the liquid phase chip and the kit, and belongs to the technical field of gene chips. The SNP molecular marker combination and the liquid chip provided by the invention can be used for efficient and rapid genetic typing of corn germplasm resources, and are low in cost and suitable for corn biological breeding. The SNP marker provided by the invention uniformly covers the whole corn reference genome, provides a high-quality molecular marker for silage corn whole genome selective breeding, and can also be used for map-based cloning of important agronomic trait genes of silage corn.

A method comprising receiving a transaction request to print a genetic sequence, the transaction request including an account and a key, a state of the account managed by a blockchain where a plurality of copies of the blockchain include a state of the account; retrieving a stored key associated with the account; authenticating the transaction request based on the stored key and the transaction request key; determining a state of the account in a first copy of the blockchain is the same as in at least a second copy of the blockchain; validating the transaction request based on the determining a state of the account in a first copy of the plurality of copies of the blockchain is the same as in at least a second copy of the plurality of copies of the blockchain; and authorizing the transaction request based on the authentication and validation of the transaction request.

Aspects of the invention include methods of obtaining associated images and sequence data of a single cell, such as a single cell of a cell sample. Embodiments of the method include combinatorily barcoding cells with a specific binding member / oligonucleotide barcode to produce combinatorily barcoded cells, e.g., obtained from an initial cell sample. The resulting combined barcoded cells are then partitioned to produce combined barcoded single cells each having a partition of a combined barcode. Image data and sequence data of the partitioned combined barcoded single cells are then obtained, and then the image data and sequence data sharing the common combined barcode are correlated to obtain correlated image and sequence data of the single cells of the cell sample. Compositions for practicing the methods of the invention are also provided.

The invention discloses a correction method of PCR (Polymerase Chain Reaction) preference, a DNA (Deoxyribose Nucleic Acid) artificially synthesized sequence combination of a TCR-beta immune repertoire, a primer combination for amplifying the TCR-beta immune repertoire, a quantitative analysis method of the TCR-beta immune repertoire of a gDNA sample, a detection kit and application of the detection kit. The PCR preference correction method can effectively correct the preference generated in the PCR amplification process, the DNA artificially synthesized sequence combination can effectively adjust the primer concentration ratio of the multiple PCR primer combination, and the primer combination can efficiently and quantitatively amplify a TCR-beta immune repertoire without mutual interference as much as possible. According to the method, the immune repertoire can be accurately and quantitatively analyzed at the DNA level.

Embodiments of systems, methods, and compositions provided herein relate to methods of simultaneously analyzing multiple analytes in a single sample using a single assay. Some embodiments relate to simultaneous analysis of DNA and RNA in a single sample, for example, to the simultaneous generation of DNA and RNA libraries.

The present disclosure relates to compositions, methods, processes, kits and devices for selecting, designing, preparing, manufacturing, formulating and / or using polynucleotides having internal ribosome entry site (IRES) sequences, IRES-like sequences, or combinations thereof. In particular, the present disclosure relates to compositions, methods, processes, kits and devices for selecting, designing, preparing, manufacturing, formulating and / or using cyclic polynucleotides (e.g., cyclic RNAs). The present disclosure also relates to methods of improving expression, functional stability, immunogenicity, ease of manufacture and / or half-life of therapeutic products encoded by the circular RNA.

The invention provides a larimichthys crocea fast-growing character associated SNP marker combination and breeding application, the combination is prepared from a probe for detecting 10 SNP molecular marker combinations of larimichthys crocea fast-growing characters, and information of positions of all SNP molecular markers on chromosomes, alleles with changed sites and reference genes is shown in a table 1. According to the SNP marker combination disclosed by the invention, the optimal large yellow croaker individuals with fast-growing potential and a certain scale can be selected from the breeding offspring in advance for breeding and breed conservation, and the large yellow croaker individuals are bred to the parent without breeding and breed conservation of a large number of offspring seeds in the breeding process, so that the breeding cost can be greatly reduced. The SNP marker combination provided by the invention can solve the problem that non-lethal detection of the growth speed of the large yellow croaker cannot be realized in a traditional breeding mode, and can ensure smooth development of fast-growing character breeding work of the large yellow croaker.

The invention provides a design method of a specific gene probe for detecting pathogenic microorganisms, and belongs to the technical field of microorganism detection. Comprising the following steps: acquiring reference genome data of target pathogenic bacteria, reference genome data of all species belonging to the same genus as the target pathogenic bacteria and genome data of all strains under the target pathogenic bacteria species; comparing the reference genome data of the target pathogenic bacteria with the reference genome data of all target pathogenic bacteria congeneric species by using MUMmer to obtain all fragments in the reference genome of the target pathogenic bacteria, wherein the base number of the fragments is greater than or equal to 20 when the fragments are continuously compared with the reference genomes of other congeneric microorganisms, and the base number of the fragments is greater than or equal to 20 when the fragments are continuously compared with the reference genomes of other congeneric microorganisms; recording starting base sites and ending base sites of all fragments; breaking a reference genome of the target pathogenic bacteria into a 50nt k-mer set, and filtering out non-specific fragments in the 50nt k-mer set to obtain an initial candidate probe set; filtering the initial candidate probe set to obtain a candidate probe set 3; and filtering the candidate probe set 3 to obtain a specific probe set. According to the design method, the specific probe is directly mined in the genomic data of the pathogenic microorganisms, large-scale probe design work can be competent, the designed probe has excellent resolution, the accuracy of pathogenic microorganism detection can be improved, and accurate detection of the pathogenic microorganisms is achieved.

The present disclosure relates to methods for the production of a cell-based product based on using synthetic promoters or a cellular input-output unit thereof, configured for controlling the expression of at least one nucleic acid sequence of interest, upon sensing dynamic cellular- and / or environmental- state and / or condition, that leads to a desired phenotype of the cells. The present disclosure further provides cell-based products, in particular, animal cell-based meat products (ACBM).