755results about "Nucleotide libraries" patented technology

The disclosure provides systems and methods for droplet processing. For example, a method can include providing a first droplet and a second droplet. In some cases, the first droplet may have a first concentration of a reagent and the second droplet may have a second concentration of the reagent. The second droplet may comprise a bead or a biological particle. The method can also include subjecting the first droplet and the second droplet to conditions sufficient to transfer the reagent from the first droplet to the second droplet, thereby decreasing the first concentration in the first droplet and increasing the second concentration in the second droplet.

The invention discloses a cowpea 5K SNP (Single Nucleotide Polymorphism) liquid chip and application thereof in variety identification, and belongs to the technical field of biology. The liquid chip provided by the invention comprises a set of nucleotide probes for detecting 5847 SNP sites of cowpea, and can be widely applied to different scenes of germplasm resource identification, genetic diversity evaluation, variety identification, genetic relationship identification, purification and rejuvenation of local varieties and the like of different cowpea varieties; an effective scientific basis is provided for cowpea variety identification, the variety identification and evaluation efficiency is improved, and the method has a remarkable application value.

The invention belongs to the field of molecular biological identification, and particularly relates to a specific SNP (Single Nucleotide Polymorphism) site combination for identifying a Wuzhishan pig variety and application. The specific SNP site combination for identifying the Wuzhishan pig variety comprises 77 SNP sites, and the physical positions of the SNP sites are determined by sequence comparison based on a pig reference genome Sscrofa11.1. The specific SNP site combination is screened based on a method of combining whole genome association analysis with selection signal analysis so as to ensure the accuracy of site selection. The selected specific SNP site combination can rapidly realize accurate identification of the Wuzhishan pig variety on the gene level, and has significant application value in the aspects of genetic resource accurate protection and variety utilization of Hainan Wuzhishan pigs.

The use of an SNP site combination of Litopenaeus vannamei in trait evaluation of mixed-family culture, a probe and a kit. The SNP site combination of Litopenaeus vannamei comprises 1125 SNP sites, wherein the physical positions are determined on the basis of alignment with a reference genome sequence of Litopenaeus vannamei. Also disclosed are a molecular probe for capturing the SNP site combination and a kit comprising the molecular probe. By means of using the SNP site combination for paternity identification, the present invention can achieve the mixed culture of individuals of different families in the early stage, so that the common environmental effect caused by separate family culture in the early stage is effectively reduced, and the accuracy of a trait evaluation result of mixed culture is improved; moreover, the genetic relationship between individuals can be accurately calculated. Compared with physical marks, the present invention avoids the phenomena of falling and dislocation of marks on individuals and human identification errors, thus obtaining accurate pedigrees.

The present disclosure relates to methods for the production of a cell-based product based on using synthetic promoters or a cellular input-output unit thereof, configured for controlling the expression of at least one nucleic acid sequence of interest, upon sensing dynamic cellular- and / or environmental- state and / or condition, that leads to a desired phenotype of the cells. The present disclosure further provides cell-based products, in particular, animal cell-based meat products (ACBM).

The invention relates to a high-throughput cis-acting element screening carrier and a screening method. Specifically, the invention provides a plasmid vector system containing bar codes, each bar code in the system is in one-to-one correspondence with a candidate cis-acting element, and the activation multiple of the candidate cis-acting element can be obtained by measuring the abundance of the bar codes; in order to eliminate the influence of a bar code on the vector on a detection result, an exogenous intron which can be cut off during transcription is inserted into a coding region in the vector and is used for distinguishing vector DNA and RNA obtained by transcription. The screening method of the biological cis-acting element has high efficiency, wide applicability and high throughput, and has outstanding application value in biological research and breeding.

The invention belongs to the technical field of molecular biology, and particularly discloses a cotton 10K functional site breeding chip and application thereof. The breeding chip is named as' Cotton Core No.1 ', and comprises a 10K functional site targeting capture probe group designed based on a reference genome of disease-resistant, high-quality and high-yield modern upland cotton No.8, the probe group comprises 11159 SNP (Single Nucleotide Polymorphism) loci, 3981 of the SNP loci are functional loci associated with 13 important agronomic characters, and 7178 of the SNP loci are background loci reflecting genetic diversity; the SNP site information is shown in the specification table 1. The invention discloses a cotton 10K functional site breeding chip and application thereof, the breeding chip contains functional sites with more index function genetic variation, has higher detection accuracy among different cotton varieties, is applied to cotton molecular marker-assisted selection breeding, whole genome selection breeding and whole genome correlation analysis, and has higher detection accuracy. The breeding efficiency can be obviously improved, and the practical application is wider.

The invention discloses a chip for genome breeding and variety identification of tilapia mossambica. The invention provides a group of SNP (Single Nucleotide Polymorphism) marker combination for tilapia mossambica, which comprises 50000 SNP markers which are respectively SNP1-SNP50000 markers. According to the invention, the SNP marker combination related to economic characters of tilapia mossambica is integrated, representative sites with uniform coverage are selected, a biological probe for site typing is developed, and the site coverage, typing accuracy and GS accuracy of the SNP marker combination are basically consistent with those of re-sequencing; the method can be applied to the aspects of tilapia germplasm resource identification, genetic relationship identification, SNP typing, variety identification, molecular breeding, DNA fingerprint database construction, variety purity detection, germplasm resource genetic analysis, whole genome selective breeding, functional gene positioning, genetic map construction, genetic evolution analysis, whole genome association analysis and the like.

The invention provides an SNP molecular marker combination for paternity test and individual recognition of dairy cows and application, the SNP molecular marker combination comprises 300 SNP markers on 29 autosomes of the dairy cows, the marker combination can be applied to paternity test and individual recognition of the dairy cows, and an identification method comprises the step of preparing a liquid phase chip of the 300 SNP markers, constructing a high-throughput sequencing library by using the genome DNA of the dairy cow to be detected; mixing the liquid phase chip probe with a high-throughput sequencing library, capturing a DNA fragment containing a target SNP site in the dairy cow DNA high-throughput sequencing library, amplifying and purifying to obtain a genetic typing of a dairy cow individual to be detected, and performing paternity test inference according to the genetic typing. The invention provides a probe and a kit for identifying SNP (Single Nucleotide Polymorphism) site information. According to the invention, the SNP polymorphism is detected through targeted capture sequencing, through high-depth sequencing, the typing result is accurate and reliable, and the accuracy of paternity test and individual recognition is ensured.

The invention discloses a Chinese rose vase life whole genome SNP molecular marker combination and application thereof, the SNP locus combination comprises 1679 SNP markers significantly associated with Chinese rose vase life, and the SNP markers are uniformly distributed on 14 chromosomes of Chinese rose. The SNP loci are obtained through genome-wide association analysis and screening, a genome-wide selection model constructed by the SNP loci can accurately predict the vase life of the Chinese rose, and the prediction accuracy can reach 0.710 or above. The invention also relates to specific probes designed aiming at the SNP loci and a liquid phase breeding chip containing the probes, which can be used for rapidly and efficiently screening individuals with excellent vase life characters in a Chinese rose seed or seedling stage, so that the breeding period is remarkably shortened, the breeding efficiency is improved, and the breeding cost is reduced. Powerful technical support is provided for molecular breeding and variety improvement of the Chinese roses.

The invention relates to a method for producing nano-arrays and micro-arrays. The method described herein provides a method of generating an array of spatially separated proteins. The method relies on covalently attaching each of a plurality of proteins to a structured nucleic acid particle (SNAP) and attaching the SNAP to a solid support.

The invention discloses a 5 'UTR library, a recombinant expression vector and a genetically engineered bacterium for improving the yield of folamin in pathogenic bacterium Badei and application of the 5' UTR library and the genetically engineered bacterium, and belongs to the technical field of microbial genetic engineering. The method comprises the following steps: designing an SD region based on a 16S rRNA (ribosomal Ribonucleic Acid) 3'end complementary sequence of a Buddei pathogenic bacterium XBD101 serving as an original strain, regulating and controlling the number of bases between the SD sequence and an initiation codon AUG and optimizing an UTR secondary structure to construct a library containing 14 UTR sequences, and replacing three sequences N4A, N7A and N18A with the strongest translation ability in the library to the UTR region of a folamin synthesis key gene fclC, so as to obtain the folamin peptide. And finally, the capacity upgrading of the folamin chassis strain is realized, and an important support is provided for the industrial production of agricultural antibiotics.

The invention discloses a China rose inflorescence whole genome SNP molecular marker combination and application thereof. A traditional breeding method for improving China rose inflorescence characters is time-consuming and labor-consuming, and ideal plants are difficult to obtain quickly. The SNP molecular marker technology is utilized, 358 Chinese rose materials and inflorescence phenotype data are analyzed through whole genome association, 1574 SNP loci remarkably related to inflorescence are screened out, a whole genome selection model with the prediction accuracy larger than 0.743 is constructed, and inflorescence characters can be effectively predicted. Meanwhile, a corresponding probe is synthesized to be used for capturing and sequencing, the capturing efficiency is high, the coverage depth is good, the SNP sites related to the rosa chinensis inflorescence can be accurately detected, an efficient technical means is provided for rosa chinensis genetic improvement and new variety cultivation, and the development of the rosa chinensis industry is assisted.

The invention discloses a Chinese rose petal outer edge wave whole genome SNP (Single Nucleotide Polymorphism) molecular marker combination and application thereof, and aims to solve the problems that the traditional Chinese rose breeding period is long, the Chinese rose breeding is easily interfered by the environment, and the petal wave character cannot be predicted in a seedling or seed stage. According to the method, 358 Chinese rose whole genome re-sequencing data and petal outer edge wave phenotype association analysis are integrated, a multi-model combined screening strategy is adopted, 1453 SNP markers obviously associated with petal outer edge waves are determined, and a whole genome selection model with high prediction precision is constructed. By combining a specific probe and chip technology, high-throughput and rapid detection is realized, the detection period can be shortened, the petal wave traits can be directly predicted by utilizing genotype data in a seedling stage, the breeding process is accelerated, and core data support is provided for Chinese rose petal outer edge wave genetic law analysis and molecular marker-assisted breeding; the method has outstanding theoretical value and application potential.

An object of the present invention is to provide methods of discovering drugs effective for tough targets, which have conventionally been discovered only with difficulty. The present invention relates to novel methods for cyclizing peptide compounds, and novel peptide compounds and libraries comprising the same, to achieve the above object.

The present invention relates to a library comprising a plurality of detection probes suitable for detection of a set of analytes of interest, each detection probe comprising an analyte-specific binding domain and a nucleic acid moiety, the nucleic acid moiety of a detection probe being capable of generating a reporter nucleic acid molecule comprising an identification sequence identifying the respective analyte of interest, said library being wherein the library comprises, for one or more analytes of interest, a set of two or more detection probes wherein each detection probe within the set is capable of generating a reporter nucleic acid molecule comprising an identification sequence that differ from the identification sequences generated by the other members of the same set. The invention further relates to a method for composing a panel with use of the library, as well as a computer program implementing said method.

The application relates to the technical field of gene chips, in particular to a SNP molecular marker combination of Pinus massoniana and application thereof. The SNP molecular marker combination is composed of 113,709 SNP molecular markers, wherein the physical position of the SNP molecular markers is determined by sequence alignment based on a reference genome Pinus.tabuliformis V1.0 of Pinus tabulaeformis. The application provides a whole genome chip of Pinus massoniana containing 113,709 SNP sites for the first time, and the chip has the advantages of high efficiency, low cost, good genetic stability and the like, and can be widely applied to different application scenarios such as identification of Pinus massoniana germplasm resources, genetic background analysis of breeding materials, gene positioning, whole genome association analysis, whole genome selection breeding and intelligent design breeding.

The present description provides a cancer assay panel for targeted detection of cancer-specific methylation patterns. Further provided herein includes methods of designing, making, and using the cancer assay panel for diagnosis of cancer.

The invention discloses a China rose fragrance whole genome SNP molecular marker combination and application thereof, 2068 SNP markers significantly related to fragrance are screened out by analyzing 358 parts of China rose re-sequencing materials and flower fragrance phenotype data, and a whole genome selection model is constructed. The method can accurately predict the fragrance character of the China rose, improve the breeding efficiency and accuracy, shorten the breeding period and reduce the cost. Meanwhile, a new direction is provided for China rose fragrance improvement and breeding, the method has reference value for character improvement of other horticultural crops, has efficient and stable capture efficiency, and can be used for subsequent sample detection and evaluation.

PURPOSE: To provide a biochip and a manufacturing method for the same.SOLUTION: A detection unit of a biochip includes a substrate, a first insulating layer, a semiconductor layer, a second insulating layer, a first metal layer, a second metal layer, and a protection layer. The semiconductor layer includes a reaction region. The second insulating layer is disposed on the semiconductor layer, and includes a first part, a second part, and a first opening part for exposing the reaction region. The first metal layer is disposed on the second part and includes a source, a drain, a gate, and a first wall structure. The second metal layer includes a second wall structure disposed on the first wall structure. The protection layer is disposed on the first metal layer and the second metal layer. The protection layer includes a second opening part, a third opening part, and a fourth opening part, and includes a flat part that defines the second opening part by surrounding it, a first projection part that defines the third opening part by surrounding it, and a second projection part that defines the fourth opening by surrounding it. In a normal direction of the substrate, the first opening part, the second opening part, the third opening part, and the fourth opening part overlap with each other.SELECTED DRAWING: Figure 4

The invention belongs to the technical field of biology, and discloses a cattle SNP chip and application thereof, the detection object of the liquid phase chip comprises 7,829 SNP sites located on a common cattle reference genome AR-UCD 1.2, and the position information of the SNP sites is shown in the specification table 3. The liquid chip provided by the invention highlights the specificity of the local cattle variety in the Tibet autonomous region, and by analyzing the local cattle population structure, sites capable of effectively distinguishing the population are screened, so that the problems of strong universality and poor specificity of a commercial chip are solved. The liquid-phase chip can also realize flexible selection and upgrading of sites, and breaks through the limitation that a solid-phase chip cannot flexibly supplement or update the sites. The whole method can be stably operated on the premise of limited sample size and complex population structure, and is suitable for genetic resource protection and application scenarios of rare varieties. The method can also be expanded into a standardized typing tool, and is suitable for various applications such as local cattle germplasm registration, variety identification, region identification and the like.

The present invention discloses a whole-genome low-density chip for the selection of high-yield and long-lived traits in dairy cows and its application. The present invention first discloses a whole-genome low-density chip for the selection of high-yield and long-lived traits in dairy cows, comprising 10,000 SNP sites, with ARS-UCD1.2 as the reference genome, and the information of the 10,000 SNP sites is shown in Table 6. The present invention further discloses the application of the above-mentioned whole-genome low-density chip. The 10,000 SNP sites contained in the whole-genome low-density chip of the present invention are relatively evenly distributed on the chromosomes, covering the whole genome and important trait sites, which can effectively improve the accuracy of longevity and milk production selection, and can be applied to gene screening, gene positioning, marker-assisted breeding and other directions. In addition, the detection cost of the low-density chip of the present invention is greatly reduced, and it is more suitable for use in commercial dairy farms, which is of great significance to dairy cow breeding and dairy cow population improvement.

The present invention provides systems and methods for sequencing cell free nucleic acids to assess a condition. Generally, methylation sequencing for specific regions associated with aberrant methylation is performed using a cell free nucleic acid sample. Based on the results of the sequencing, methylation of the cell free nucleic acid molecule can be assessed. Various features may be derived from methylation assessment and used within a computational model to assess a cell free nucleic acid sample for a condition.

The invention discloses a Chinese rose petal fold whole genome SNP (Single Nucleotide Polymorphism) molecular marker combination and application thereof. 968 SNP markers obviously related to petal fold traits are screened out and distributed on 14 chromosomes by analyzing 358 parts of Chinese rose re-sequencing data and utilizing a plurality of software models to carry out whole genome association analysis. A liquid-phase gene chip is designed on the basis of the SNP loci, and plants with ideal petal folding characters can be rapidly screened out through DNA detection in the Chinese rose seedling stage. The liquid phase breeding chip has the advantages of high throughput detection, low cost, shortened breeding period and the like, can simultaneously detect a large number of SNP sites and reduce the cost, can predict future performance of characters in a seed or seedling stage, greatly shortens the breeding period, accelerates cultivation of a new Chinese rose variety with a unique petal rolling character, and has a wide application prospect. The requirements of the market for novel flower types are met, the ornamental value and market competitiveness of the Chinese rose variety are improved, and the development of the flower industry is promoted.

The invention belongs to the technical field of molecular biology, bioinformatics, sorghum genetics and whole-genome gene chips, and particularly relates to a sorghum whole-genome SNP molecular marker combination developed based on a targeted sequencing technology, a 10K liquid chip and application of the sorghum whole-genome SNP molecular marker combination and the 10K liquid chip. The SNP molecular marker combination comprises 10,000 SNP loci, and the specific information of the 10,000 SNP loci is as shown in a table 1; the physical positions of the 10000 SNP loci are determined by performing sequence alignment based on a reference genome of the wine red tassel sorghum variety HYZ-T2T with the version number of v1.0. A liquid chip developed based on the SNP molecular marker combination provided by the invention can be effectively applied to genetic diversity analysis, whole genome association analysis, genetic typing, molecular marker-assisted selective breeding, whole genome selective breeding and other applications of sorghum germplasm resources.

Disclosed herein are devices and methods for isolation and analysis of exosomal molecules from cell DNA samples. Such devices and methods facilitate isolation of ecDNA for downstream applications, such as cellular analysis and design of probes or therapeutic agents, in order to detect or target ecDNA for degradation or cells carrying ecDNA. Similarly, disclosed herein are compositions enriched for ecDNA and their use in the development of therapeutic or diagnostic agents.

Systems and methods for sequencing of cell-free nucleic acids to assess a condition are provided. Generally, a cell-free nucleic acid sample is utilized to perform methyl sequencing targeted to particular regions associated with aberrant methylation. Methylation of cell-free nucleic acid molecules can be assessed based on the sequencing result. Various features can be derived from the methylation assessment and utilized within a computational model to assess the cell-free nucleic acid sample for a condition.

A composition is reported that includes a pair of a first nucleic acid and a second nucleic acid, the first nucleic acid including, in that order, a first portion of a stem nucleic acid sequence, a cleavage site, a first stem-loop nucleic acid sequence, the 5' and 3' portions of which form a duplex, a first portion of a catalytic core sequence, a second stem-loop nucleic acid sequence, the 5' and 3' portions of which form a duplex, a second portion of the catalytic core sequence, and a second portion of the stem nucleic acid sequence that is complementary to, and thus forms a duplex with, the first portion of the stem nucleic acid sequence, and the second nucleic acid is complementary to at least a portion of the first or second portion of the stem nucleic acid sequence, and upon binding of the second nucleic acid to the first nucleic acid, results in a conformational change in the first nucleic acid, the conformational change being at least one of dissociation of the first portion of the first stem sequence from the second portion of the stem sequence and hybridization of one of said portions with the second nucleic acid, or dissociation of Loop I and Loop II, resulting in inactivation of catalytic activity, or association of Loop I and Loop II, resulting in activation of catalytic activity.

To provide a gene testing method and a gene testing kit for analyzing a variable region of a TCR or a BCR in single-cell analysis.SOLUTION: A method including a step of capturing mRNA eluted from a single cell using an RT probe immobilized on a solid-phase surface and including a common sequence, a cell identification barcode, a first molecular identification barcode, and an oligo(dT) sequence, a step of performing a reverse transcription reaction in a reverse transcription reaction solution containing a TSO using the mRNA as a template to generate cDNA having a sequence added to a 3' end, a step of degrading the mRNA, a step in which a primer for inserting a second molecular identification barcode binds to a constant region adjacent to a variable region of a TCR or a BCR and includes the second molecular identification barcode, a step of ligating cDNA extended from the primer with a ligase, a step of amplifying a fragment including the second molecular identification barcode and the variable region of the TCR or the BCR using a primer, a step of performing sequence analysis, and a step of associating sequences.SELECTED DRAWING: Figure 2

Described herein are methods of preparing dual-indexed nucleic acid libraries for methylation profiling using bisulfite conversion sequencing. In various embodiments, the methods use a two-step indexing process to tag bisulfite-treated DNA with unique molecular identifiers (UMIs).