144 results about "Biosecurity" patented technology

The present application relates to the technical field of aquaculture monitoring and regulation, and discloses a water quality multi-parameter real-time online monitoring system. The system comprises a multi-source data acquisition module for obtaining and denoising water quality data; a time sequence feature analysis module for generating multi-dimensional feature clusters containing the correlation of various parameters; an anomaly identification module for dynamic deviation compensation, monitoring sub-domain demarcation and key parameter extraction; a collaborative regulation module for constructing a regulation decision unit to generate a multi-parameter collaborative regulation strategy; and an iterative correction module for correcting the regulation strategy to output real-time adjustment instructions. An early warning module is also provided for biological safety assessment and early warning and activation of the emergency intervention mechanism. The system can accurately monitor the multi-parameters of the water quality of aquaculture in real time, realize intelligent regulation and biological safety early warning, effectively improve the yield and quality of aquaculture, reduce the risk of aquaculture, and promote the sustainable development of the industry.

The invention belongs to the technical field of data processing, and relates to a disinfectant verification method based on Jerlin codes. The invention aims to solve the problem of biological potential safety hazards that the identity and the state are separated in the anti-counterfeiting verification of the existing disinfectant, and the titer of the liquid medicine falls off the threshold value due to environmental factors cannot be identified. The method comprises the following steps: in response to a code scanning operation, analyzing a Jerlin code to obtain a factory fingerprint, and splicing cloud logistics and terminal storage data to form a full-link environment monitoring sequence; calculating an environment pressure acceleration coefficient of each time slice by using a nonlinear kinetic model, and evaluating the damage degree of the environment to chemical properties; calculating accumulated chemical loss and residual titer based on the coefficient; and comparing the residual titer with a threshold value, and if the residual titer reaches the standard, generating a dynamic hash token containing titer information for cloud verification. According to the invention, deep combination of physical efficacy and digital verification is realized, and the use safety and reliability of the disinfectant are greatly improved under the condition that an expensive sensor is not needed.

The invention relates to the technical field of livestock and poultry breeding epidemic prevention, and discloses a biological safety system suitable for a breeding community, comprising: a space partition and hardware facility module for constructing a physical framework; the four dynamic line closed-loop management modules are used for executing a one-way flow standard operation instruction; the environment pressure gradient regulation and control module is used for maintaining stable air pressure gradients in areas with high safety levels to low safety levels, the integrated monitoring module is used for acquiring real-time data through a sensor network, and the biological safety state interlocking management and control module decides according to the real-time data, automatically issues instructions and regulates and controls hardware facilities and the environment pressure gradients in a linkage mode, so that intelligent active defense is achieved. Spatial partition, hardware facilities, dynamic line processes, environment regulation and control and intelligent interlocking management and control are systematically integrated, a set of multi-level and closed-loop comprehensive biological security defense system is constructed, and the defect of systematic prevention and control vulnerabilities in the prior art is overcome.

The invention discloses a clinical laboratory specimen full-process tracking and biosafety early warning management system, and relates to the technical field of medical biosafety management, and the system comprises a multi-modal data collection module which integrates various devices and comprehensively collects specimens and related data; the intelligent identification and verification module adopts an advanced algorithm to process the image, completes compliance judgment and traceability verification, and outputs a comprehensive data packet; the dynamic risk assessment module calculates a risk coefficient and matches a five-color early warning level; the grading early warning and linkage disposal module determines an early warning grade, quantifies disposal intensity, and realizes visual management and control and closed loop; according to the method, accurate determination of specimen compliance and full-process traceability verification are realized through deep analysis of an algorithm, data quality and management accuracy are improved, meanwhile, a dynamic risk assessment mechanism is constructed, risks are graded accurately, disposal intensity is quantified, risk visual management and control and closed-loop management are realized, and normalization and controllability of biological safety management are enhanced.

The invention discloses a method for regulating out-of-season breeding of large yellow croakers bred in autumn. The method is characterized in that spawning in spring and summer is achieved through a three-stage technical system of biological safety prevention and control, precise environment regulation and control and reproductive physiological intervention. The method specifically comprises the following steps: 1) detecting trypanosomes; 2) parent fish screening; (3) parent fish rearing; 4) artificial propagation; 5) artificial incubation; and 6) seedling cultivation. According to the method, the out-of-season spring and summer breeding technology breakthrough of breeding the large yellow croaker in autumn is successfully achieved through nutrient enrichment, accurate temperature parameter regulation and control, gradual illumination regulation and reasonable hormone treatment. According to the technology, an adjustable reproduction synchronization scheme is provided for cross breeding between a spring reproduction group and an autumn reproduction group, and the technology has important application value for germplasm innovation of the large yellow croaker.

The utility model discloses a high-fidelity quick sampling device of a high-grade biological safety dissecting room, which relates to the technical field of biological sampling devices, and comprises a barrel, a first end of the barrel is provided with a cavity, an inlet communicated with the cavity is arranged at the first end of the barrel, scale marks are arranged outside the barrel, a first cutting part is arranged at the first end of the barrel, and a second cutting part is arranged at the second end of the barrel. The first cutting part has a cutting state and an idle state; the operation part is connected with the cylinder body, the operation part and the inlet are arranged in an avoiding manner, and a barrier layer is arranged between the operation part and the cylinder body; the blocking layer enables substances such as pathogens not to be attached to the operation part, the operation part is detachably connected with the second end of the cylinder body and the blocking layer, and therefore the situation that the pathogens attached to the cylinder body and the blocking layer are attached to the next biological tissue, errors are caused to follow-up identification and analysis of the next biological tissue can be avoided as far as possible. And high fidelity during sampling of different biological tissues is realized.

Disclosed herein is a waste encapsulation method comprising: (i) placing an adhesive composition into a compactor; (ii) placing one or more flexible containers containing waste material into the compactor; (iii) heating the binder composition to liquefy the binder composition and embedding the waste into the liquefied binder composition to form a coated mixture; and (iv) compacting the coating mixture and forming a hydrophobic composite article containing the waste material.

The present application relates to the field of genetic engineering, and in particular to a vector for analyzing plant promoter expression specificity, a preparation method and application thereof. The T-DNA region of the vector comprises, from 5' end to 3' end, a callus-specific promoter-driven selection marker gene expression cassette and a reporter gene expression cassette with a multiple cloning site; the callus-specific promoter-driven selection marker gene expression cassette comprises a callus-specific promoter, a selection marker gene coding sequence and a terminator. The vector of the present application utilizes a rice callus-specific promoter to drive the expression of a selection marker gene, can greatly reduce the non-specific interaction with the promoter of a target gene, improve the specificity of the expression of the target gene, and effectively reduce the biological safety risk caused by the selection marker gene in a transgenic plant, and has important application value in basic theoretical research and molecular breeding.

This invention provides a negative pressure isolation live microscope experimental box. The experimental box, through its closed negative pressure design, ensures the biosafety of experiments with pathogens of BSL-2 level and above, meeting the requirements of the safety committee review. At the same time, its innovative box-type integrated window structure eliminates the vibration torque caused by animal movement in traditional suspended windows from a mechanical design perspective, providing unparalleled stability for high-precision imaging. Ultimately, it makes it possible to safely and stably conduct live microscopic studies of highly pathogenic infection models in a conventional laboratory.

This invention provides a method for monitoring and evaluating the risk of pathogenic bacteria transmission in poultry farm feces, belonging to the field of livestock and poultry biosecurity control technology. The method includes evaluating the risk of Salmonella transmission during the poultry physiological cycle, assessing the risk of antibiotic resistance associated with drug administration behavior, and monitoring the function during composting based on microbial community structure and metabolic function. This invention can accurately locate the highest-risk nodes of pathogen contamination and antibiotic resistance evolution during the breeding cycle, solving the problem of inaccurate identification of risk sources in existing technologies and avoiding poultry product contamination due to missed risk assessments. It overcomes the single-dimensional limitation of existing technologies that only detect the quantity of pathogens, comprehensively evaluating pathogen risk from phenotypic, molecular, and functional levels. The assessment results are more in line with the actual biosecurity needs of poultry farming, ultimately reducing the safety risks of poultry product contamination and the spread of antibiotic resistance.

The invention provides a biological safe and efficient fattening field, and belongs to the technical field of livestock breeding. A negative pressure isolation belt is formed by the outer shell and the inner shell, so that external air is forced to flow through the visible light catalysis quantum dot layer to realize pathogen inactivation while the gas flow rate is controlled. An optical fiber sensing siphon is arranged below the slatted floor to monitor sewage pathogenic bacteria in real time. Live pigs enter the house through the third-stage buffer channel. The system breaks through the limitation of a traditional physical barrier, full-path blocking of a transmission chain of epidemic diseases such as African swine fever is achieved, and the biological safety level is improved by two orders of magnitude.

The invention discloses an intelligent monitoring method, device and equipment for livestock breeding and a storage medium, and relates to the technical field of intelligent monitoring of livestock breeding, and the method comprises the steps: S100, data collection and time synchronization; s200, carrying out adaptive denoising and cross-modal feature extraction; s300, carrying out individualized baseline modeling and drift self-adaption; s400, performing multi-scale event association and weak causal identification; s500, risk grading and executable intervention suggestion generation; according to the method, priority disposal decision and resource optimization are oriented to economic and biological safety, namely, path confidence scores and node influence probabilities are integrated into calculation of influence ranges and expected intervention cost, and confidence-driven uncertainty premium is reflected in the cost, so that alarms not only reflect biological risks, but also reflect uncertain premium in the cost; the potential economic influence and the operation cost can be quantified; based on the multi-dimensional score, the method can preferentially put limited veterinarians and epidemic prevention resources, medicines and isolation measures into a cluster which contributes to group health and productivity loss risk to the greatest extent.

The invention relates to the technical field of animal epidemic disease prevention and control and farm biosafety, in particular to a safe reproduction method for an African swine fever virus nucleic acid positive pig farm. A pollution area is determined through accurate risk assessment and gridding dynamic management, environment purification is achieved by combining thorough cleaning and disinfection with hardware upgrading and process reconstruction, and finally, a positive field is successfully converted into a negative field capable of achieving full-load stable production by relying on a triple guarantee system of environment monitoring, microbiological assessment and sentinel pig biological verification. According to the invention, through accurate risk assessment and dynamic grid management, visual positioning of a pollution area is realized; high-efficiency cleaning and disinfection are combined with hardware upgrading and process reconstruction, so that viruses are thoroughly removed, and transmission is blocked; based on environmental nucleic acid, microbial culture and sentinel pig triple verification, biological safety and reliability are ensured. Finally, the positive field is successfully converted into a negative field for stable production, and a systematic and reproducible safe reproduction scheme is provided for the industry.

The invention discloses an LAMP (loop-mediated isothermal amplification) primer group, a kit and a method for detecting four quarantine viruses and application of the LAMP primer group. Belongs to the technical field of molecular biology and plant quarantine. Aiming at conserved gene regions of bean pod mottle virus, tobacco ringspot virus, tomato ringspot virus and southern bean mosaic virus, a specific target sequence is screened through bioinformatics analysis, an LAMP primer group is designed, and the nucleotide sequence of the LAMP primer group is shown as SEQ ID NO.1-22. Furthermore, the screened primer group is combined with a micro-fluidic chip technology to construct a detection kit capable of synchronously detecting the four viruses, so that the specific identification of the four target viruses is realized. The kit has the advantages of rapidness, sensitivity, specificity, simplicity and convenience in operation and the like, the sensitivity can reach 10 <-10 > Copies / mu L, and the kit is suitable for high-throughput rapid screening of quarantine viruses in imported soybeans on port sites and has important significance in guaranteeing national biosafety.

This invention discloses recombinant humanized type III collagen, its preparation method, and its applications, belonging to the field of medical materials technology. The amino acid sequence of this recombinant humanized type III collagen is any one of SEQ ID No. 2-6. Compared to traditional extraction methods, the amino acid sequence of this recombinant protein is highly consistent with that of natural human proteins, completely avoiding the biosafety risks of pathogens, prions, etc., that may exist during animal-derived extraction, while also exhibiting lower immunogenicity. Furthermore, this protein effectively maintains the natural biological activity and stability of collagen. When used as a wound dressing, this material can efficiently promote cell migration, spreading, and proliferation, accelerate tissue regeneration and wound healing, while possessing excellent biocompatibility, controllable degradation, and low immunogenicity.

This invention relates to the fields of environmental monitoring and biosafety technology, and in particular to a viral aerosol sampling patch for face masks, comprising: a capture layer for intercepting viral aerosol particles in the air; a culture layer disposed inside the capture layer, the culture layer comprising a culture unit supporting the virus to maintain activity and replicate in the presence of a host, a host bacterium sensitive to the virus, and a visual indicator indicating viral replication or host response; and a base layer disposed inside the culture layer; the sampling patch is fixed to the face mask through the base layer, and viral aerosol collection is achieved without external power through the capture layer, and countable detection results are generated after sampling through the culture layer; utilizing the airflow and humidity conditions naturally formed during wearing, effective interception, activity preservation, and visual detection of viral aerosols are achieved without complex equipment and additional operating steps, and the operation is simple and repeatable.

The invention discloses a stacked caged laying hen breeding management method and system, a terminal and a medium, and relates to the technical field of breeding management. According to the main technical scheme, individual physiological status information of all laying hens in a breeding area is obtained, and when the individual physiological status information does not meet a preset health status threshold value, a physical mark is attached to the body surface of a target laying hen to be processed; and processing information is generated according to the identification information of the physical marks and the breeding position information of the breeding area where the target laying hens to be processed are located. The method is expected to achieve the purposes of finding the abnormal health state of the laying hens in an early stage, further conveniently and quickly isolating the abnormal health laying hens, greatly reducing the risk of cross infection, improving the biological safety level of a henhouse and reducing the outbreak probability of group epidemic situations. And meanwhile, the aims of enabling the laying hens with abnormal health to be visualized and recognizable in a dense group, so that a worker can quickly lock specific abnormal individuals in the dense group during subsequent processing, thereby shortening the positioning time of the worker and improving the response speed are fulfilled.

The invention relates to a medical xenon recovery system based on multistage filtration and dynamic separation. The medical xenon recovery system comprises a pretreatment and microorganism inactivation module, a gas purification and separation module, a xenon circulation distribution module, an intelligent monitoring system and a dynamic pressure compensation mechanism. The invention further relates to a medical xenon recovery method based on multi-stage filtration and dynamic separation, bacteria are intercepted through the filter screen, viruses are intercepted through large particles, short-wave sterilization ultraviolet LED array irradiation is combined, double barriers of physical interception and ultraviolet inactivation are formed, the method is far superior to a traditional single filtration technology, the grade cleaning standard is met, and the medical xenon recovery method has the advantages of being simple in operation, low in cost and high in efficiency. The system thoroughly solves the biological potential safety hazard of lack of ultra-small virus interception and inactivation in the prior art, can efficiently inactivate respiratory pathogens, can effectively prevent microorganisms in exhaled air from circulating to patients or medical staff through a recovery system, is especially suitable for infectious surgery scenes, and remarkably reduces the risk of cross infection in hospitals.

The application discloses a highly immature duck Tembusu virus, a culture method and application thereof, and belongs to the technical field of biotechnology.According to the method for culturing the highly immature duck Tembusu virus based on the LOVO cell line provided in the application, the duck Tembusu virus obtained by culture loses pathogenicity, the application provides a highly potential candidate strain for developing safe and effective duck Tembusu virus vaccines, opens up a new way for safely producing high-quality diagnostic antigens and vaccine antigens, and significantly reduces the biosafety risk of related research.

The invention relates to the technical field of biological safety and ecological protection, and discloses a method for tracing an alien invasive plant solidago canadensis along with diffusion of nursery stocks. Respectively collecting and recording geographic coordinates, foundation soil color, plant species in a foundation soil range and specification attribute data of nursery stocks; and identifying the color of the foundation soil by using three attributes of colors by adopting a Morse color system. Data comparison and analysis: adopting a three-step analysis strategy to accurately identify the source of solidago canadensis carried by the nursery stock; according to the traceability result, the diffusion risk of solidago canadensis is evaluated, and targeted prevention and control suggestions of source prevention and early-stage monitoring and early warning are provided. According to the established traceability analysis method, interregional specificity based on four types of data including root soil, associated plant species, meter diameter and branch height can be identified, and the accuracy of a traceability result is ensured.

The utility model discloses a microorganism monitoring device for a biosafety laboratory, and relates to the technical field of microorganism monitoring devices, the microorganism monitoring device for the biosafety laboratory comprises a probe shell, a mounting cavity is formed in the probe shell, and the mounting cavity extends out of the front side surface of the probe shell; a high-definition probe is fixedly connected to the interior of the mounting cavity, heat dissipation holes are formed in the rear side surface of the high-definition probe, a conical concentration groove is formed in the inner wall of the rear side of the mounting cavity, a transmission channel is fixedly connected to the inner wall of the rear side of the conical concentration groove, and a first connecting column is fixedly connected to the rear side surface of the probe shell; hot air exhausted by the heat dissipation holes can be directly guided to the outside through the corrugated heat conduction pipe, so that the exhausted hot air is prevented from influencing the living environment of microorganisms, meanwhile, the corrugated heat conduction pipe and the transmission channel are cooled through the spiral cooling channel, heat overflow is avoided, and the stability of the living environment of the microorganisms is further guaranteed.

The present application relates to pigsty washing control technical field. The purpose is to provide a kind of for biosecurity management's washing control system, including center server and several sets of with center server communication connection, installed in pigsty pigsty environment monitoring device and washing execution device;The washing execution device includes excrement pushing mechanism, fence, and washing mechanism.This system can monitor the environment in pigsty in real time, and realize the automatic washing of pigsty and pig, with the characteristics of automation, intelligent level is high, meet the needs of modern pig farm.The whole washing process is based on full automation, with washing cloud control, remote mobile terminal control and washing dynamic push function, with the advantages of high degree of interconnection and high level of information.

The utility model relates to the technical field of livestock breeding, and discloses an automatic uniform mixing type medicine adding device for a plug tray stockline, which comprises a stirring box arranged on the plug tray stockline; the stirring assembly comprises a plurality of stirring pieces and a plurality of conveying pieces, the stirring pieces and the conveying pieces are arranged in the stirring box, the stirring pieces and the conveying pieces are arranged in a staggered mode and connected end to end, the inlet end of the stirring piece located at one end communicates with one end of the tray plugging material line, and the discharging end of the conveying piece located at the other end communicates with the other end of the tray plugging material line; the medicine adding assembly comprises a medicine adding bin and a medicine adding part, the medicine adding bin is fixedly connected to the stirring box and communicates with the inlet end of the stirring part located at one end, and the medicine adding part is arranged in the medicine adding bin and used for controlling medicine output. Normal conveying of feed in a feed line is not affected, the feed can be fully mixed with medicine after passing through the device, and the problems that manual medicine adding is high in cost, low in efficiency and not beneficial to biological safety protection and the medicine and the feed cannot be fully mixed are solved.

The application discloses a kind of high-temperature sterilization treatment processes and systems of sick and dead livestock and poultry, belong to sick and dead livestock and poultry processing technical field, this kind of high-temperature sterilization treatment processes and systems of sick and dead livestock and poultry, including ground mechanism, hydraulic flap mechanism, crushing mechanism, transport mechanism, processing mechanism and gas degradation mechanism.The application is realized through the setting of ground mechanism, hydraulic flap mechanism, crushing mechanism and processing mechanism, contactless transfer and low manual intervention in the process of sick and dead livestock and poultry processing, to significantly reduce the risk of cross contamination, improve operation efficiency and epidemic prevention reliability, the application is realized through the setting of processing mechanism, gas degradation mechanism and flow guide mechanism, closed loop processing of sick and dead livestock and poultry, high-temperature sterilization kills pathogen completely, guarantees biological safety, and realizes low energy consumption, environmental protection stability, airflow uniform distribution and high mass transfer efficiency of biodegradation, whole closed no leakage, epidemic prevention and environmental benefits are remarkable, safe and reliable operation.

The application discloses a reinforced secondary biological safety laboratory intelligent integrated management system, which comprises a control terminal, a system login module, a system setting module, a user management module, a project management module, a laboratory management module, an animal room management module, an instrument and equipment management module, a project statistics module and a log management module. The application is a kind of efficient, convenient and professional system management operation software, which is used for biological safety laboratory project management, laboratory reservation use, experiment personnel management, experimental animal and cage site management, laboratory facility and equipment and consumable management, and realizes intelligent, digital and whole-process integrated management.

The invention belongs to the technical field of agricultural biology, and particularly relates to a rice endogenous space-time specific promoter OsSULTR1; the invention discloses 3pro and application thereof. The promoter OsSULTR1 is characterized in that the promoter OsSULTR1 The nucleotide sequence of 3pro is as shown in SEQ ID NO. 1. The invention provides an OsSULTR1; 3pro is a space-time specific expression regulation element from rice, can efficiently and stably drive a target gene to be expressed in a plant callus, and can realize targeted efficient and stable expression in root and stem tissues in a plant vegetative growth period. The promoter can effectively replace a non-plant-source promoter commonly used in existing plant genetic engineering, has an important industrial application value in the field of plant genetic engineering, and not only can improve the targeting and stability of target gene expression in transgenic plants, but also can improve the yield of the transgenic plants. And the potential biological safety risk of the transgenic plant caused after the exogenous DNA is introduced into the plant genome can be obviously reduced.

This invention discloses a portable UV and hydrogen peroxide co-sterilization device for biosafety sample collection and delivery packaging. The device employs a double-layered insulated enclosure, with a UV sterilization lamp at the top and an atomizing hydrogen peroxide nozzle on the inner wall. The atomizing nozzle sprays micron-sized aerosol particles to achieve omnidirectional dispersion of the aerosol medium within the sealed space, combined with the UV light source arrangement design to eliminate sterilization dead zones. An adjustable partition inside the enclosure allows for flexible separation of samples of different sizes, preventing cross-contamination. Ventilation holes in the enclosure walls balance internal and external air pressure, ensuring product safety. An environmental monitoring module at the bottom monitors temperature and humidity data in real time and displays it on a screen outside the enclosure, providing visualization of the internal temperature and humidity. This invention facilitates dual sterilization and is adaptable to various pathogenic microorganism disinfection scenarios, possessing characteristics such as impact resistance, corrosion resistance, non-destructive sample handling, and environmental friendliness.

The present application relates to the technical field of livestock safety management, and particularly relates to a digital management system and method for biological safety access of a breeding pig farm. The system is cooperatively operated by a management platform, a mobile terminal, a video monitoring system and an Internet of Things device, controls the whole process of personnel, vehicles, materials, food and feed entering and leaving the breeding pig farm, and builds an integrated closed-loop management mechanism of "plan, execution, supervision, rectification and data". Through plan reporting and approval, the orderly arrangement of access activities is realized. Through job reporting and standard execution, the key operation process is standardized. Through video monitoring and parameter collection, the whole process is supervised. Through the negative list and rectification mechanism, a closed loop of problem processing is formed. Through data collection and report analysis, the access management information is visualized and decision support is realized. The method can effectively improve the biological safety management level, reduce the risk of disease transmission and improve the efficiency of breeding pig breeding.

The present application relates to the technical field of biological safety and ecological protection, and discloses a method for tracing the spread of alien invasive plant Solidago canadensis with nursery stock, which comprises the following steps: collecting and recording the geographic coordinates, root base soil color, plant species in the root base soil range, and specification attribute data of the nursery stock in the planting area of the nursery stock suspected to carry Solidago canadensis and the spread points caused by the transportation of the nursery stock; identifying the color of the root base soil by using the Munsell color system and three attributes of color; and adopting a three-step analysis strategy to accurately identify the source of Solidago canadensis carried by the nursery stock, evaluating the spread risk of Solidago canadensis according to the tracing result, and putting forward targeted prevention and control suggestions for source control and early monitoring and early warning. The tracing analysis method can identify the region-specificity of the four types of data, i.e., root base soil, associated plant species, diameter, and branch height, thereby ensuring the accuracy of the tracing result.

This invention relates to a high-efficiency aerosol sampling device and method, belonging to the fields of environmental monitoring and biosafety technology. It includes a U-shaped ventilation tube with a flat bottom, an air inlet at the top of the left longitudinal tube, and an air outlet at the top of the right longitudinal tube. A fan is installed at the air inlet to achieve high-flow-rate air introduction. The inner side of the flat section of the U-shaped ventilation tube is filled with sampling liquid, and a temperature control component is installed on the outer side of the flat section. This invention provides a device and method that can significantly increase the contact area through an evaporation-condensation cycle without reducing airflow, achieving high-throughput and high-efficiency microbial aerosol sampling. It solves the problems of low airflow, limited contact area between aerosol and sampling liquid, and insufficient sampling efficiency in existing aerosol sampling technologies.