41 results about "Biosecurity" patented technology

This invention provides a negative pressure isolation live microscope experimental box. The experimental box, through its closed negative pressure design, ensures the biosafety of experiments with pathogens of BSL-2 level and above, meeting the requirements of the safety committee review. At the same time, its innovative box-type integrated window structure eliminates the vibration torque caused by animal movement in traditional suspended windows from a mechanical design perspective, providing unparalleled stability for high-precision imaging. Ultimately, it makes it possible to safely and stably conduct live microscopic studies of highly pathogenic infection models in a conventional laboratory.

This invention provides a method for monitoring and evaluating the risk of pathogenic bacteria transmission in poultry farm feces, belonging to the field of livestock and poultry biosecurity control technology. The method includes evaluating the risk of Salmonella transmission during the poultry physiological cycle, assessing the risk of antibiotic resistance associated with drug administration behavior, and monitoring the function during composting based on microbial community structure and metabolic function. This invention can accurately locate the highest-risk nodes of pathogen contamination and antibiotic resistance evolution during the breeding cycle, solving the problem of inaccurate identification of risk sources in existing technologies and avoiding poultry product contamination due to missed risk assessments. It overcomes the single-dimensional limitation of existing technologies that only detect the quantity of pathogens, comprehensively evaluating pathogen risk from phenotypic, molecular, and functional levels. The assessment results are more in line with the actual biosecurity needs of poultry farming, ultimately reducing the safety risks of poultry product contamination and the spread of antibiotic resistance.

This invention discloses recombinant humanized type III collagen, its preparation method, and its applications, belonging to the field of medical materials technology. The amino acid sequence of this recombinant humanized type III collagen is any one of SEQ ID No. 2-6. Compared to traditional extraction methods, the amino acid sequence of this recombinant protein is highly consistent with that of natural human proteins, completely avoiding the biosafety risks of pathogens, prions, etc., that may exist during animal-derived extraction, while also exhibiting lower immunogenicity. Furthermore, this protein effectively maintains the natural biological activity and stability of collagen. When used as a wound dressing, this material can efficiently promote cell migration, spreading, and proliferation, accelerate tissue regeneration and wound healing, while possessing excellent biocompatibility, controllable degradation, and low immunogenicity.

This invention relates to the fields of environmental monitoring and biosafety technology, and in particular to a viral aerosol sampling patch for face masks, comprising: a capture layer for intercepting viral aerosol particles in the air; a culture layer disposed inside the capture layer, the culture layer comprising a culture unit supporting the virus to maintain activity and replicate in the presence of a host, a host bacterium sensitive to the virus, and a visual indicator indicating viral replication or host response; and a base layer disposed inside the culture layer; the sampling patch is fixed to the face mask through the base layer, and viral aerosol collection is achieved without external power through the capture layer, and countable detection results are generated after sampling through the culture layer; utilizing the airflow and humidity conditions naturally formed during wearing, effective interception, activity preservation, and visual detection of viral aerosols are achieved without complex equipment and additional operating steps, and the operation is simple and repeatable.

The application discloses a kind of high-temperature sterilization treatment processes and systems of sick and dead livestock and poultry, belong to sick and dead livestock and poultry processing technical field, this kind of high-temperature sterilization treatment processes and systems of sick and dead livestock and poultry, including ground mechanism, hydraulic flap mechanism, crushing mechanism, transport mechanism, processing mechanism and gas degradation mechanism.The application is realized through the setting of ground mechanism, hydraulic flap mechanism, crushing mechanism and processing mechanism, contactless transfer and low manual intervention in the process of sick and dead livestock and poultry processing, to significantly reduce the risk of cross contamination, improve operation efficiency and epidemic prevention reliability, the application is realized through the setting of processing mechanism, gas degradation mechanism and flow guide mechanism, closed loop processing of sick and dead livestock and poultry, high-temperature sterilization kills pathogen completely, guarantees biological safety, and realizes low energy consumption, environmental protection stability, airflow uniform distribution and high mass transfer efficiency of biodegradation, whole closed no leakage, epidemic prevention and environmental benefits are remarkable, safe and reliable operation.

The present application relates to the technical field of livestock safety management, and particularly relates to a digital management system and method for biological safety access of a breeding pig farm. The system is cooperatively operated by a management platform, a mobile terminal, a video monitoring system and an Internet of Things device, controls the whole process of personnel, vehicles, materials, food and feed entering and leaving the breeding pig farm, and builds an integrated closed-loop management mechanism of "plan, execution, supervision, rectification and data". Through plan reporting and approval, the orderly arrangement of access activities is realized. Through job reporting and standard execution, the key operation process is standardized. Through video monitoring and parameter collection, the whole process is supervised. Through the negative list and rectification mechanism, a closed loop of problem processing is formed. Through data collection and report analysis, the access management information is visualized and decision support is realized. The method can effectively improve the biological safety management level, reduce the risk of disease transmission and improve the efficiency of breeding pig breeding.

This invention relates to a high-efficiency aerosol sampling device and method, belonging to the fields of environmental monitoring and biosafety technology. It includes a U-shaped ventilation tube with a flat bottom, an air inlet at the top of the left longitudinal tube, and an air outlet at the top of the right longitudinal tube. A fan is installed at the air inlet to achieve high-flow-rate air introduction. The inner side of the flat section of the U-shaped ventilation tube is filled with sampling liquid, and a temperature control component is installed on the outer side of the flat section. This invention provides a device and method that can significantly increase the contact area through an evaporation-condensation cycle without reducing airflow, achieving high-throughput and high-efficiency microbial aerosol sampling. It solves the problems of low airflow, limited contact area between aerosol and sampling liquid, and insufficient sampling efficiency in existing aerosol sampling technologies.

The application discloses a kind of based on water body virus load and fish body tissue virus load correlation grouper nervous necrosis virus nondestructive testing method.The method is through optimizing the concentration condition of virus in aquaculture water, realize the efficient concentration and quantification of water body virus load, then combine water-water aquaculture animal virus load correlation model, indirectly and nondestructively assess the virus infection state of aquaculture animal.The application detects water environment instead of detecting animals itself, realizes nondestructive, dynamic, early warning monitoring of the health status of aquaculture animal population, with the advantages of low cost, simple operation, frequent implementation, etc., and has important application value in aquatic disease prevention and control, good seed selection and biological safety management.

The present application discloses a kind of tray class object cleaning equipment, to solve the problem of secondary pollution in existing cleaning technology, high energy consumption and big biological safety risk.It includes frame body and gas collection cover body, cover in sequence along conveying device setting cleaning station, rinsing station and purging station.And set a set of closed loop air collection purification system based on single fan: fan from cleaning station through negative pressure suction pipe suction contaminated air, the air enters the closed fan cover after demisting, adsorption and dehumidification;Fan uses its own operating waste heat to heat the inhaled clean air, and sends it to the purging station as high-temperature purge gas flow;Most of the purge gas flow returns to the cleaning station to form an internal circulation in the cover, and forms a negative pressure barrier at the inlet, thereby controlling the dispersion of water mist, aerosol and odor from the source.The present application has the advantages of compact structure, zero dispersion control of pollutants, significant environmental protection, energy saving and safety.

This invention discloses a tobacco alanine aminotransferase gene, NtALAAT2, and its applications, belonging to the fields of plant genetic engineering and tobacco genetic breeding technology. The nucleotide sequence of the NtALAAT2 gene is shown in SEQ ID NO.1, and the amino acid sequence of its encoded protein is shown in SEQ ID NO.2. This invention reveals for the first time that the expression level of the endogenous NtALAAT2 gene in tobacco is negatively correlated with proline content. By reducing the expression level of this gene, the proline content in tobacco can be effectively increased, thereby enhancing the tobacco's tolerance to drought and / or soil salinization stress, without significantly adversely affecting the normal growth and development of the plant. This invention employs an endogenous gene regulation strategy, avoiding the biosafety risks of introducing exogenous genes, and provides a new gene resource and efficient technical pathway for breeding new tobacco varieties with high stress resistance.

This invention relates to a digital monitoring method, system, and medium for biosecurity in pig houses, belonging to the field of pig house monitoring technology. The invention utilizes data statistics and key indicator calculations based on control data to generate a visual dashboard. Through spatiotemporal aggregation analysis of multi-source data on pigs, it identifies the spatial and temporal clustering of abnormal pigs and encodes multi-dimensional features to form a high-dimensional feature vector. Based on this high-dimensional feature vector, real-time risk monitoring and early warning are performed, and contact network tracing is conducted to generate tracing results. Based on the tracing results, propagation path simulation is performed to generate optimal decision-making suggestions, which are then displayed according to a preset method. This invention, through early detection and early intervention, can reduce the risk of disease spread in breeding pig farms and reduce losses such as pig deaths and production stoppages caused by epidemics. Calculations show that annual losses per farm can be reduced by 15%-30%.

This invention discloses a biosafety-compliant independent ventilation system. The air circulation pipe of the cage frame is equipped with a first docking hole and a first valve core assembly at the corresponding installation position, while the cage box is equipped with a second docking hole and a second valve core assembly. Both valve core assemblies employ an elastic reset and conduction structure. During cage box installation, the two valve stems push against each other and move in opposite directions to connect the connection channels. During disassembly, the elastic element drives the valve stems to reset and seals the docking holes with a sealing element. The air circulation device is connected to the air circulation pipe to deliver clean air and exhaust waste gas, while the disinfection device is connected to the air circulation device to deliver disinfection media for in-situ sterilization. This invention achieves precise control of airflow through the cooperation of the two valve core assemblies, solving the problems of serious biosafety hazards during ventilation docking and the existence of sterilization blind spots in the disinfection system.

This application relates to a method for tracing the source of abnormal events in biological laboratories based on unified spatiotemporal data, aiming to improve laboratory safety and avoid potential biohazard risks. The method includes: deploying multiple aerosol monitoring points and location monitoring devices within the laboratory to acquire aerosol concentration and staff behavior trajectory data; constructing a health status model based on aerosol concentration data under normal operating conditions, and comparing it in real time to determine whether there is a leakage risk; if a leakage risk exists, determining the key area and propagation path of the aerosol leak using monitoring data, and identifying staff whose time and area of ​​contact with the leak overlap spatiotemporally. Through multi-point monitoring and deep fusion of spatiotemporal data, this method can accurately, efficiently, and in real time trace leakage events, especially in high-level biosafety laboratories, effectively improving safety protection capabilities.

The application provides an ISO5-level clean hatching method for SPF ducks and belongs to the technical field of animal breeding equipment.The method solves the problems of the traditional hatching equipment, such as insufficient cleanliness, high pollution risk, poor environmental control precision and the inability to meet the biological safety and sterile breeding requirements of SPF ducks.The method comprises the following steps: 1: introducing air from the outside and sequentially performing three-stage progressive purification treatment on the introduced air through a multi-stage air inlet filtering structure (9) of a hatcher; 2: sending the three-stage purified clean air into a hatching bin (1) of the hatcher in a vertical laminar flow mode; 3: sending an egg tray vehicle (13) carrying SPF duck eggs into the hatching bin (1); and 4: operating personnel operate the SPF duck eggs in the hatching bin (1) through a glove operation system (4) installed on the hatching bin (1) under the condition of maintaining the positive pressure and cleanliness in the hatching bin (1). The method is mainly used for the breeding of poultry, such as SPF ducks, which do not carry specific pathogenic bacteria.

A biosafety laboratory is disclosed. This laboratory comprises multiple inflatable membrane structure virus detection laboratories. The inflatable membrane structure of each laboratory forms a high-contamination zone. Transfer units are suitable for being installed at the first and second transfer ports of this high-contamination zone. The inflatable membrane structure includes an inflatable membrane dome structure, and the functional space is defined by the inflatable membrane dome structure after inflation. The inflatable membrane dome structure includes an outer support frame and an inner single-layer membrane, which is adapted to form a positive-pressure air-membrane space between itself and the support frame. The second transfer port of one of the inflatable membrane structure virus detection laboratories is aligned with the first transfer port of an adjacent inflatable membrane structure virus detection laboratory and connected via a transfer unit. The inflatable membrane structure can be inflated to form a roof or tubular structure, facilitating the rapid construction of the inflatable membrane structure virus detection laboratory and enabling it to be put into use as soon as possible.

The application discloses a pig farm introduction optimization method and system based on artificial intelligence. First, the production parameters and real-time data of each breeding area of the target pig farm are obtained, a production state time transfer matrix of the sow group is constructed, and the required sow redundancy or introduction quantity and time of each batch in the future are predicted accordingly. Second, the biological safety and health data of the sow group in each area are obtained, and the clustering algorithm is used to calculate the comprehensive health state similarity of the pig groups between different areas in terms of disease history, immune state and real-time health indicators. Finally, the predicted data and the health similarity between areas are comprehensively considered to generate an introduction optimization scheme including an internal sow allocation plan and an exogenous introduction plan. The application realizes data-driven introduction and allocation precision decision-making, and can optimize the pig group structure, guarantee biological safety and improve production efficiency.

This invention discloses a method for assessing the microbial safety risk of water bodies based on metagenomics and viromics, comprising: metagenomic detection of the relative abundance F of each pathogenic microorganism in the water sample to be assessed. i Where i represents the pathogenic microorganism label; for pathogenic microorganism i, determine its hazard level PM. i Antibiotic resistance gene risk value (ARG) i and virulence factor risk value (VF) i ; Determine the risk coefficient α of increased transmission due to water sample treatment methods, including antibiotic resistance gene risk values ​​and virulence factor risk values; For pathogenic microorganism i, determine the overall hazard level PM i Antibiotic resistance gene risk value (ARG) i Virulence factor risk value (VF) i The risk coefficient α and relative abundance F of increased transmission due to water sample treatment methods i Determine the overall risk value R of pathogenic microorganism i i The comprehensive risk value of all pathogenic microorganisms detected by the metagenomics is used to obtain the microbial safety risk assessment result R of the water sample to be evaluated.

The present application relates to a kind of plasmid for endowing bacillus to degrade skatole function and its application, belong to agricultural environment microorganism and genetic engineering technical field, to solve the existing skatole management high cost, easy to produce secondary pollution, part of degradation strain exists biological safety risk, and the genetic element of natural degradation strain is difficult to cross-strain application problem.The present application discloses the plasmid of nucleotide sequence as shown in SEQ ID NO.1, by the plasmid is transformed into bacillus competent cell, is cultivated in M9 solid medium containing skatole and positive clone detection obtains recombination bacillus, it is inoculated with 0.5% to 5% inoculation amount in the medium containing 0.05 to 0.2g / L skatole, under the condition of 30 to 37 DEG C, 120 to 250rpm 4 to 48h can be degraded skatole.The present application is mainly used for the skatole deodorization of breeding manure and the like scene, provides safe controllable, efficient and scalable biological technology scheme.

The application discloses a pig farm introduction optimization method and system based on artificial intelligence. First, the production parameters and real-time data of each breeding area of the target pig farm are obtained, a production state time transfer matrix of the sow group is constructed, and the required sow redundancy or introduction quantity and time of each batch in the future are predicted accordingly. Second, the biological safety and health data of the sow group in each area are obtained, and the clustering algorithm is used to calculate the comprehensive health state similarity of the pig groups between different areas in terms of disease history, immune state and real-time health indicators. Finally, the predicted data and the health similarity between areas are comprehensively considered to generate an introduction optimization scheme including an internal sow allocation plan and an exogenous introduction plan. The application realizes data-driven introduction and allocation precision decision-making, and can optimize the pig group structure, guarantee biological safety and improve production efficiency.

The utility model relates to color selection device technical field, concretely is a kind of small soybean color selection device, including shell, first pulse air valve and second pulse air valve are arranged in the shell, and two are staggered spatial layout, the surface of the shell is provided with first discharge channel, the first pulse air valve is used to spray ordinary impurities to first discharge channel, the surface of the shell is provided with second discharge channel, the second pulse air valve is used to spray target risk object to second discharge channel, the surface of the shell is provided with third discharge channel, the utility model's purpose is to solve when in customs quarantine, target risk object needs to be strictly isolated and needs to be destroyed according to biological safety process, traditional color selection machine uses single -way waste collection design, two kinds of substances need artificial secondary sorting after mixing to carry out compliance processing to target risk object, increase the problem of operation complexity.

The present application belongs to the field of biological safety technology, and particularly relates to a biological safety cabinet control method, a biological safety cabinet and a storage medium. The present application aims to solve the problem of high cost and low monitoring accuracy of the wind speed in the biological safety cabinet, thereby affecting the use effect. The biological safety cabinet control method comprises the following steps: determining the opening and closing states of the door body and the exhaust port of the biological safety cabinet; and determining the working state of the biological safety cabinet according to the opening and closing states of the door body and the exhaust port. The present application can reduce the cost of detecting the inflow wind speed of the biological safety cabinet, and the whole detection accuracy is high, thereby further improving the user experience.

This invention relates to the field of detection and provides a bioaerosol sampling solution. The bioaerosol sampling solution comprises the following components in the following proportions: a chelating agent, a buffer, a surfactant, a protein denaturant, a humectant, and a solubilizer. The sampling solution with specific components of this invention can directly achieve a lysis effect, thereby integrating the nucleic acid extraction step into the collection step, reducing existing sampling operations, reducing system losses, and achieving highly efficient collection results. Simultaneously, the sampling solution with specific components of this invention has excellent bactericidal effects on Gram-negative and Gram-positive bacterial solutions of different concentrations, effectively preventing microbial toxicity and personnel infection during the sampling process. Furthermore, when using the sampling solution of this invention for sampling, there is no need for repeated removal / replacement, further ensuring the potential biosafety of operators.

This invention discloses a biological invasion identification method and system based on multi-source data fusion analysis, specifically relating to the fields of artificial intelligence and biosafety monitoring technology. It addresses the problem that existing knowledge graph-based identification methods struggle to maintain path semantics and contextual constraints during multi-hop relationship reasoning, leading to generalized conclusions and inaccurate risk identification. By integrating heterogeneous data from multiple sources to construct a multimodal knowledge graph with evidence tracing capabilities, and dynamically extracting focused subgraphs to limit the analysis scope when co-occurrence of target entities is detected, multi-hop relationship paths are extracted from the subgraphs, and their convergence of independent evidence sources and time window matching are comprehensively evaluated. Based on the evaluation results, the paths are filtered, integrated, and sorted, ultimately achieving accurate identification of biological invasion risk types and potential diffusion directions.

The application provides a sterilizable intelligent ear tag for SPF pigs and a health monitoring method thereof, and relates to the technical field of livestock internet of things. The ear tag comprises an ear tag female tag, an ear tag male pin and an intelligent sensing and communication module. The ear tag female tag has a fully sealed cavity and is made of a material resistant to high-pressure steam sterilization. The ear tag male pin is locked with the ear tag female tag. The intelligent sensing and communication module is sealed and contained in the fully sealed cavity. The method uses the ear tag to collect historical ear temperature and activity data to establish a dynamic health baseline of the pig. When the real-time data deviates from the baseline to reach an abnormal condition, a warning is given, and linkage with a facility biosecurity early warning system is supported. The application solves the problem that the existing ear tag cannot resist repeated high-pressure steam sterilization in the SPF barrier environment.

This invention provides a one-step, fully enclosed method for amplifying Mycobacterium tuberculosis DNA using a tongue swab, belonging to the field of Mycobacterium tuberculosis nucleic acid detection technology. The method includes the following core steps: non-invasive sampling using a tongue swab; breaking off the swab tip into a sampling tube pre-filled with lysis buffer; connecting the sampling tube to a PCR tube containing a built-in lyophilized bulb to form a fully enclosed device; squeezing the sampling tube to mix the swab with the lysis buffer to release the Mycobacterium tuberculosis DNA; inverting the device to allow the mixture to drip into the PCR tube to dissolve the lyophilized bulb; finally, inserting the PCR tube into a detection instrument, and obtaining a positive or negative result directly within 30 minutes. This invention achieves non-invasive and convenient sampling, fully enclosed safety protection, simplified one-step operation, and rapid diagnosis, without relying on a biosafety level 2 laboratory or requiring specialized technical training for operators, effectively solving the problems of difficult sampling, slow detection, and high risk in grassroots tuberculosis screening.

The application belongs to the technical field of biological detection, and discloses a pig delta coronavirus lateral flow immunochromatographic detection test strip based on nanobodies and application thereof. The test strip takes PDCoV nucleocapsid protein as a detection target, adopts a sandwich type immunodetection principle, and the core recognition element is two high-affinity nanobodies recognizing different epitopes of PDCoV N protein. 62Nb is fused with a pig IgG Fc fragment to form 62Nb-pFc, and colloidal gold is coupled as a detection probe; 94Nb is fixed on the T line as a capture antibody, and the C line is fixed with a goat anti-pig IgG. The nanobodies are prepared by a Pichia pastoris secretion expression system. The test strip is simple to operate and does not require instruments, and can complete visual detection of PDCoV in complex samples such as pig fecal swabs and breeding environment wastewater in 10-15 minutes. The test strip has high sensitivity, strong specificity, good stability, good batch consistency, and is suitable for on-site screening and biological safety monitoring in breeding farms.

This application discloses a method, equipment, medium, and product for identifying and assessing biological risks in ship ballast water, relating to the fields of marine engineering and environmental safety technology. The method includes: obtaining ship ballast water samples; screening and identifying hazardous substances based on the ballast water samples; determining the concentration values ​​of the identified hazardous substances using qPCR; constructing a risk assessment model; using the risk assessment model to perform risk substance analysis simulation based on the concentration values ​​of the hazardous substances, obtaining simulation results of ballast water biological risk substances; and obtaining ballast water biological risk assessment results based on the simulation results. This application can construct a risk management system for identifying and assessing ballast water quality safety risks to address new challenges in biosafety.

This utility model relates to the technical field of inactivation tank devices, and in particular to a biosafety double-door animal carcass inactivation tank device, including an A-zone laboratory, a B-zone laboratory, and a partition wall. The partition wall is set between the A-zone laboratory and the B-zone laboratory. Below the A-zone laboratory and the B-zone laboratory is an underground inactivation room, which also includes an isolation room, an airtight door device, and a dissection tray device. An isolation room spanning the A-zone laboratory and the B-zone laboratory is set on the partition wall. An inactivation tank is set in the underground inactivation room. The top of the inactivation tank extends to the bottom of the inner side of the isolation room. An inlet is set at each of the left and right ends of the isolation room. An airtight door device is set at each end of the isolation room. The two sets of airtight door devices seal one set of inlets. A dissection tray assembly is set on each set of airtight door devices. This device reduces experimental costs while ensuring experimental effectiveness.