221 results about "Viral test" patented technology

The invention discloses a hybridoma cell strain, a monoclonal antibody, a linear epitope antigen and application thereof, and relates to the field of genetic engineering, in particular to a hybridoma cell strain, a monoclonal antibody, a linear epitope antigen and application thereof. The preservation number of the hybridoma cell strain P27-A1 is CGMCC (China General Microbiological Culture Collection Center) No. 46352. The hybridoma cell strain P27-A1 is prepared from feline leukemia virus p27, and a monoclonal antibody secreted by the hybridoma cell strain P27-A1 recognizes a linear epitope antigen of feline leukemia virus p27 protein; the kit for the feline leukemia virus p27 comprises the monoclonal antibody A1 secreted by the hybridoma cell strain P27-A1. The monoclonal antibody secreted by the hybridoma cell strain can greatly improve the sensitivity of the p27 protein for detecting feline leukemia virus. The invention provides the monoclonal antibody secreted by the hybridoma cell strain for identification, and the monoclonal antibody can be used for preparing a diagnostic kit such as a colloidal gold test strip.

The present invention belongs to the field of virus detection technology, and specifically relates to monoclonal antibodies with neutralizing activity against adenovirus type 5 and their uses. The monoclonal antibody 8B9 of the present invention can specifically recognize and neutralize adenovirus type 5, and the amino acid sequences of the heavy and light chain variable regions and their complementarity determining regions of the monoclonal antibody 8B9 are provided. The present invention also provides an in vitro neutralization activity assessment model based on HEK-293A cells. At a concentration as low as 3.3 μg / mL, the titer of the neutralizing activity against the virus stock solution after 6000-fold dilution is 7×10 4 TCID 50 / mL of virus has close to 100% neutralizing activity. After further humanization, it is expected to be developed into a neutralizing antibody drug for the treatment of diseases such as severe pneumonia caused by HADV-5 infection, filling the current lack of effective treatment options in this field and possessing significant scientific research value and clinical application prospects.

The present invention provides a rabies virus G protein antigen epitope targeting completely human monoclonal antibody and applications thereof, the completely human monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region has three complementary determining region amino acid sequences: GDISSSCFY, IHYSGST and ARHRRGYCYDSEKGGTNWFDP, and the light chain variable region has three complementary determining region amino acid sequences: GDISSSCFY, IHYSGST and ARHRRGYCYDSEKGGTNWFDP. The amino acid sequences of the three complementary determining regions of the light chain variable region are respectively as follows: QGISND, ATS and LQDYEFPLT. The fully human monoclonal antibody has efficient and broad-spectrum anti-rabies virus neutralizing activity, is high in expression, fully human-derived and good in stability, and can be used for preparing rabies virus detection products or drugs for preventing and treating rabies.

The invention is applicable to the technical field of virus detection, and provides a norovirus detection kit based on a photo-thermal isothermal amplification technology and a detection method, the norovirus detection kit comprises a nucleic acid amplification chamber, the nucleic acid amplification chamber is provided with reaction micropores for nucleic acid amplification; an RT-PRA system, a CRISPR-cas12a system, a blank control and a positive control are placed in the reaction micropore, the RT-PRA system comprises RT-PRA solutions R1-R5, and the CRISPR-cas12a system comprises CRISPR-Cas12a solutions C1-C3; and the photo-thermal film covers the nucleic acid amplification chamber and is used for converting sunlight into heat energy and radiating the heat energy to liquid in the reaction micropores so as to provide reaction temperature for nucleic acid amplification. According to the invention, the portable, rapid and accurate detection of norovirus infection can be realized, the detection cost can be effectively reduced, the application scene is wide, workers can be helped to deduce the virus transmission path, and key support is provided for investigation and analysis of norovirus epidemic situations.

The invention provides a reagent for detecting avian leukosis virus, a nano-enzyme immunochromatography test strip and application of the nano-enzyme immunochromatography test strip, and belongs to the technical field of immunology. Aiming at the problems that the existing avian leukosis virus detection method is complicated in operation, time-consuming or strong in equipment dependence, the reagent comprises a capture antibody and a detection antibody; a heavy chain variable region of the monoclonal antibody A comprises an amino acid sequence as shown in SEQ ID NO: 1, and a light chain variable region of the monoclonal antibody A comprises an amino acid sequence as shown in SEQ ID NO: 2; a heavy chain variable region of the monoclonal antibody B comprises an amino acid sequence as shown in SEQ ID NO: 5, and a light chain variable region of the monoclonal antibody B comprises an amino acid sequence as shown in SEQ ID NO: 6; the antigen binding fragment is a Fab fragment, an F (ab) 2 fragment or a single-chain Fv fragment. The reagent is mainly used for detecting avian leukosis virus, and is suitable for detecting samples such as cloaca swab, meconium, egg white or serum of poultry.

The invention provides application of high-concentration colloidal gold in preparation of an influenza virus detection test strip, and relates to the technical field of immunodetection. The detection test strip provided by the invention is composed of a sample pad, a combination pad, a nitrocellulose membrane, a water absorption pad and a bottom plate, and in the preparation process of the detection test strip, high-concentration colloidal gold with the OD value of 100 is adopted for respectively marking an influenza A virus monoclonal antibody 1, an influenza B virus monoclonal antibody 1 and a chicken IgY antibody; optimized colloidal gold labeled reconstitution fluid is mixed and then sprayed on the combination pad, and meanwhile, a corresponding capture antibody and a goat anti-chicken IgY antibody are respectively sprayed on the nitrocellulose membrane to form a detection line and a quality control line. Through the synergistic effect of the high-concentration colloidal gold mark, the optimized sample pad / combination pad treating fluid and the special sample diluent, the detection sensitivity is remarkably improved, the false negative rate is effectively reduced, and rapid, high-sensitivity and high-specificity field detection on influenza A and B viruses is realized.

The invention discloses an indirect ELISA (enzyme-linked immuno sorbent assay) detection method of a porcine epidemic diarrhea virus antibody SIgA, and belongs to the technical field of animal antibody detection. In order to solve the problems of limited detection range and single immune evaluation dimension of a porcine epidemic diarrhea virus detection method in the prior art, the invention provides an indirect ELISA detection method of a porcine epidemic diarrhea virus antibody SIgA, and the detection method adopts a pichia pastoris eukaryotic secretion platform to prepare a high-activity PEDV S1 antigen; meanwhile, a secreting type IgA antibody is used as a detection target; the method has the advantages of high detection sensitivity, low background noise, better variant compatibility and practical popularization value, and provides a clinical and industrial-level solution for maternal immunity monitoring, piglet passive protection evaluation and vaccine immunity strategy optimization.

The invention belongs to the field of canine distemper virus detection, and particularly relates to a canine distemper virus RT-RAA CRISPR / Cas 12b detection kit and a detection method thereof. According to the kit, isothermal amplification is performed by using an RT-RAA universal primer, and then detection is performed by using a CRISPR / Cas 12b detection system consisting of target sequence sgRNA, so that amplification of a target fragment of the N gene of the canine distemper virus and target detection visualization can be effectively realized. The result of the embodiment shows that the RT-RAA CRISPR / Cas 12b detection kit finally designed by the invention is high in specificity and does not have cross reaction with other viruses, the lowest visual detection limit reaches 4.77 * 10 < 1 > copies / mu L, the virus can be instantly detected when the RT-RAA CRISPR / Cas 12b detection kit is used for detecting the CDV, the labor and equipment cost is low, the period is short, and the detection time does not exceed 2 hours. The rapid detection technology can be popularized and applied to epidemiological investigation and epidemic situation monitoring of the canine distemper virus, is suitable for epidemic disease monitoring of wild animals, quarantine of zoos and on-site rapid detection of clinical samples of canines, and has good practical significance and wide market prospects.

The invention discloses a primer pair and a fluorescent probe for detecting an infectious bronchitis virus as well as application and a detection method of the primer pair and the fluorescent probe, and relates to the technical field of biological virus detection. Wherein the nucleotide sequence of a forward primer in the primer pair is as shown in SEQ ID No.1, the nucleotide sequence of a reverse primer is as shown in SEQ ID No.2, and the nucleotide sequence of the fluorescent probe is as shown in SEQ ID No.3; the 5'end of the fluorescent probe is modified by FAM, and the 3 'end of the fluorescent probe is modified by TAMRA. By implementing the kit, IBV of various genotypes can be accurately detected, and a good basis is provided for early diagnosis of IBV.

The application discloses a reagent, a method and application of the reagent and the method for simultaneously detecting three common viruses in a fancy carp, and belongs to the technical field of fish quarantine. The application provides a reaction system and a detection method for simultaneously detecting three common viruses in a fancy carp, establishes a multiplex fluorescence PCR method, adopts a full-closed reaction, solves the problem that common PCR is prone to generating aerosols and causing false positive results of tests, has high detection sensitivity, can detect target genes with a copy number of 10 orders of magnitude at the minimum, is suitable for detecting a small amount of viruses carried in normal fancy carp or viruses in water quality environment, and can fully meet the requirements of customs quarantine prevention and control, has strong specificity, has no cross reaction to templates such as IHNV, RSIV and EHNV, and further provides a virus-like particle which can be used as a positive reference product in the multiplex system, is stable in state and beneficial to storage, can monitor an extraction process, and ensures that a test is established.

The invention discloses an anti-Ebola virus VP40 protein nano antibody and application of the anti-Ebola virus VP40 protein nano antibody in Ebola virus detection. The anti-Ebola virus VP40 protein nano antibody is a 3E07 antibody. The anti-Ebola virus VP40 protein nano antibody prepared by the invention has the characteristics of high sensitivity, wide binding spectrum and the like. A double-antibody sandwich enzyme-linked immunosorbent assay method constructed by the 3E07 and 3F06 antibodies can be used for efficiently detecting VP40 proteins and virus-like particles of various subtype Ebola viruses such as Zaire, Sudan, Bandong and the like, and the detection sensitivity for the VP40 proteins of the Zaire type Ebola viruses can reach 0.039 ng / mL; the antibody combination has a wide application prospect in diagnosis and pathogen detection of Ebola virus diseases.

The application discloses nanobodies against E protein of chikungunya virus and application in virus detection, and the nanobodies against E protein of chikungunya virus include N055 and 10G4 antibodies, and both have high affinity and binding activity with antigens. A double antibody sandwich enzyme-linked immunosorbent assay method constructed by the nanobodies of the N055 and 10G4 antibodies can efficiently detect E protein of chikungunya virus and virus-like particles, and the detection sensitivity can reach 49 pg / mL, and the antibody combination has wide application prospects in diagnosis and pathogen detection of chikungunya virus disease.

The invention provides an infectious bovine rhinotracheitis virus gD protein hybridoma cell strain, a monoclonal antibody and application of the monoclonal antibody in antigen detection, and belongs to the technical field of immunodetection. The invention provides an infectious bovine rhinotracheitis virus gD protein hybridoma cell strain IBRVMab-gD-8, and the preservation number of the infectious bovine rhinotracheitis virus gD protein hybridoma cell strain IBRVMab-gD-8 is CCTCC (China Center For Type Culture Collection) NO: The monoclonal antibody generated by the infectious bovine rhinotracheitis virus gD protein hybridoma cell IBRV Mab-gD-8 strain can specifically recognize strains of all genotypes of IBRV, does not generate cross reaction with viruses such as bovine viral diarrhea / mucosal disease virus (BVDV) and the like, and has good specificity; the kit not only can be used for exogenous virus detection (cell examination method) of IBRV in bovine virus live vaccines, but also can be used for clinical detection and epidemiological investigation of IBRV.

The invention discloses primers, a kit and a method for detecting four viruses in lentinus edodes. According to the invention, LAMP (loop-mediated isothermal amplification) primer groups are respectively designed and screened according to four viruses in the lentinula edodes, and the four viruses are respectively a Lentinula edodes dominant virus 1 virus, a Lentinula edodes dominant virus HKB virus, a Lentinula edodes dominant gene-like virus 1-Hunong-1 virus and a Lentinula edodes dominant virus 5 virus. The detection system is micro-quantized, the local concentration of the target sequence is improved from the side, the detection limit is greatly improved, and the lower detection limit can reach 10 copy / microliter.

The present invention provides neutralizing monoclonal antibodies targeting Nipah virus G protein and their uses, wherein the monoclonal antibodies can recognize Nipah virus G protein. The present invention uses NiV G protein as an antigen target, displays antigens on a ferritin nanoparticle platform to immunize mice, and screens out three monoclonal antibodies that can specifically bind to NiV G protein. Antibody epitope competition experiments found that the S1E2 and SB10 monoclonal antibodies among these three antibodies recognize new epitopes of NiV G protein that have not been reported before. In vitro neutralization experiments have demonstrated that these three antibodies have high in vitro neutralizing activity, and can neutralize both NiV-M and NiV-B strains, with the characteristics of high expression and good stability, and can be used to prepare virus detection products such as Nipah and Hendra or drugs for preventing and treating Nipah and Hendra virus diseases.

The invention discloses a Nipah virus F protein monoclonal antibody and application thereof, the antibody comprises an antibody 2D12 and an antibody 3B10, and the Nipah virus F protein monoclonal antibody is prepared from Nipah virus F protein immunogen. The specific anti-Nipah virus F protein monoclonal antibody pair is prepared and detected, the monoclonal antibody pair can be specifically combined with the Nipah virus F protein, is high in titer and good in affinity and has neutralizing activity, and meanwhile, the detection kit prepared on the basis of the antibody pair can effectively carry out Nipah virus detection; and technical support can be provided for treatment, epidemiological monitoring and diagnosis of Nipah virus infection.

The application discloses a new coronavirus strain XBB.1.5 identification method based on a surface-enhanced Raman spectrum chip, and belongs to the technical field of new coronavirus detection. The application identifies the new coronavirus strain XBB.1.5 through the detection method, avoids complex and expensive gene sequencing, can accurately determine the new coronavirus subtype, has low cost, high accuracy, short time and convenient operation.

The invention discloses a human papilloma virus mRNA (messenger ribonucleic acid) detection kit for auxiliary diagnosis of cervical cancer patients. The human papilloma virus mRNA detection kit comprises a nucleic acid probe group and a series of nucleic acid probes AMP1-AMP5, wherein the nucleic acid probe group is used for being combined with a human papilloma virus mRNA target, and the nucleic acid probes AMP1-AMP5 are used for signal amplification. The human papilloma virus mRNA detection kit provided by the invention can assist pathologists in identifying and diagnosing cervical cancer patients, and provides a basis for clinicians to formulate a therapeutic schedule.

The invention belongs to the technical field of aquatic pathogen detection, and provides a primer and probe combination with strong specificity and high sensitivity, a kit and a cdPCR detection method in order to solve the problems of dependence of absolute quantification of an existing qPCR technology on a standard curve and low repeatability and stability caused by uncontrollable quality of a standard substance. The primer probe can generate specific amplification on LYCIV and has no cross reaction on other common aquatic pathogens (such as NNV, DIV1 and the like), so that the specificity of a detection result is fundamentally ensured, a false positive result caused by the cross reaction is effectively avoided, and the diagnosis accuracy is improved. The method has the characteristics of extremely high sensitivity and absolute quantification, the lower limit of detection reaches 6.2 copies / mu L, the method has more advantages in quantification of samples with extremely low concentration, early diagnosis and detection of extremely low virus load can be realized, viruses with extremely low content in fish bodies can be detected earlier, precious time is provided for disease early warning and early intervention, and the method is worthy of popularization and application. Disease outbreak is effectively prevented.

The invention relates to the technical field of virus detection, in particular to a fluorescent RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection probe 1106VP and / or 1106WP and primers 1106VWF and 1106VWR. The invention also discloses a fluorescent RT-PCR detection reagent, a fluorescent RT-PCR detection kit, a fluorescent RT-PCR detection probe, a primer, the fluorescent RT-PCR detection reagent and the application of the fluorescent RT-PCR detection kit in identification of a porcine reproductive and respiratory syndrome NJ-1106R strain and a porcine reproductive and respiratory syndrome non-NJ-1106R strain. The probe and the primer disclosed by the invention are high in specificity and high in sensitivity; the accuracy is high, and the accuracy rate of blind sample detection is 100%; the kit is high in universality, can be used for detecting virus liquid for cell culture in a laboratory, and can also be used for detecting porcine serum and tissue samples in clinical samples. The method is an effective method for identifying the PRRSV NJ-1106R vaccine strain and other PRRSV wild strains.

The invention discloses a humanized broad-spectrum anti-HIV-1 (human immunodeficiency virus-1) monoclonal neutralizing antibody, which is obtained by screening through a single B cell flow sorting-antibody gene amplification pairing expression technology, has a unique CDR (CDR region), can be specifically combined with HIV-1, and can effectively neutralize various pseudoviruses, even Tier 3 pseudoviruses. The invention also relates to a preparation method and application of the neutralizing antibody. The antibody provided by the invention can be used for emergency prevention and / or treatment of HIV-1 variant strains, has the characteristics of full human source, high expression and good stability, and is suitable for industrialization. In addition, the antibody can also be used for preparing an HIV-1 virus detection reagent, finding effective neutralizing epitopes and developing HIV-1 vaccines.

The application discloses a DPO RT-PCR primer group for synchronously detecting five citrus viruses, a detection method, a kit and application of the DPO RT-PCR primer group, and sequences of the primer group are shown as SEQ ID NO. 1-SEQ ID NO. 10. The application designs specific DPO RT-PCR primers for citrus tristeza virus, citrus tatter leaf virus, citrus yellow vein clearing virus and citrus leaf mottle virus, and the detection primers can effectively remove primer dimer interference, and the five viruses can be detected from samples at the same time. The detection method is simple and efficient, and only needs to use a common PCR instrument and an electrophoresis instrument to detect, and has the characteristics of rapidness, economy, high accuracy and high sensitivity, and can be used for large-scale screening of field citrus plants and epidemic prevention detection before seedling transportation, and provides technical support for healthy seedling detection and field screening of diseases.

The invention belongs to the technical field of dengue virus detection, and particularly discloses a dengue virus RPA-CRISPR-Cas12b detection system and a detection method of the dengue virus RPA-CRISPR-Cas12b detection system. The invention selects a conserved region of the dengue virus as an amplification and detection target, and provides a specific sgRNA (sequence as shown in SEQ ID NO.3) and an RPA primer for detecting the dengue virus. The RPA primer is used for carrying out RPA amplification on a to-be-detected sample, specific sgRNA is used for carrying out CRISPR reaction on an amplification product, and whether the to-be-detected sample contains the dengue virus or not can be judged according to the color change of a reaction system. The invention provides corresponding sgRNA and RPA primer sequences and application, and the provided RPA-CRISPR-Cas12b detection system and detection method have the technical advantages of simple operation, rapid and sensitive reaction, strong specificity and the like, and meet the application requirements of on-site instant visual detection of dengue viruses.

The application provides a one-step RT-PCR detection kit for detecting porcine getah virus infection and application thereof, and belongs to the technical field of virus detection. The application provides a one-step RT-PCR detection kit for detecting porcine getah virus (GETV) infection and application thereof, wherein the kit contains a pair of specific primers for detecting the getah virus. The kit has good detection result specificity, and has no cross reaction with main members of pig diseases and common pig, cattle and sheep pathogens; the kit has high sensitivity, and the sensitivity for detecting plasmid standard products and GETV cell toxins is 5.9 copies and 0.3 TCID 50 respectively; the kit is rapid, sensitive, simple, low in cost, reverse transcription and PCR are performed in one reaction tube, and the pollution opportunity is reduced.

The invention relates to the technical field of biology, in particular to an enteric adenovirus hydrogel limited amplification primer combination, a kit and application of the enteric adenovirus hydrogel limited amplification primer combination. According to the present invention, the primer, the reaction reagent and the reaction system are optimized, and the hydrogel limiting amplification technology is adopted to develop the hydrogel limiting amplification reaction system suitable for enteroadenovirus detection, and the hydrogel limiting amplification reaction system is used for enteroadenovirus detection, the method is simple and rapid in steps, low in equipment requirement, capable of effectively preventing and treating aerosol pollution, good in storage stability, high in sensitivity and high in specificity.

This utility model discloses a porcine reproductive and respiratory syndrome virus (PRRSV) detection kit, relating to the field of detection kits. The PRRSV detection kit includes a kit body, a bracket at the bottom of the kit body, and a detection mechanism on the outside of the kit body. The detection mechanism includes a detection block disposed inside the kit body, a sealing block movably disposed above the detection block, and a fixing bracket movably engaged inside the bracket and movably disposed above the detection block, thus fixing the kit body and the bracket in place. This PRRSV detection kit allows for the determination of the injection site of the kit body without opening the kit body using the detection block. Subsequently, the sealing block sequentially seals the area above the kit body, and the moving fixing bracket secures all kit bodies above the bracket, reducing the complexity of this fixation method and ensuring that the use of the sealing block does not affect the overall fixation effect of the kit body and bracket.

The application discloses a nano antibody against Ebola virus VP40 protein and application thereof in Ebola virus detection, wherein the nano antibody against Ebola virus VP40 protein is a 3E07 antibody. The nano antibody against Ebola virus VP40 protein prepared by the application has the characteristics of high sensitivity and wide binding spectrum. A double antibody sandwich enzyme-linked immunosorbent assay method constructed by 3E07 and 3F06 antibodies can efficiently detect VP40 proteins and virus-like particles of various Ebola virus subtypes such as Zaire, Sudan and Beni, wherein the detection sensitivity of the Ebola virus VP40 protein of Zaire type can reach 0.039 ng / mL, and the antibody combination has a wide application prospect in the diagnosis and pathogen detection of Ebola virus disease.

This invention relates to an ELISA detection kit for porcine reproductive and respiratory syndrome virus (PRRSV) based on M-GP5 recombinant protein, and belongs to the field of ELISA detection technology. The kit includes recombinant (M-GP5) antigen protein, an ELISA plate, blocking buffer, primary antibody dilution buffer, enzyme-labeled secondary antibody, secondary antibody dilution buffer, chromogenic solution, stop solution, negative serum, and positive serum. It features high sensitivity, high specificity, and good stability, and can accurately detect PRSV antibodies.

The invention discloses a macaque cytomegalovirus detection kit supporting multi-mode interpretation and a detection method, and particularly relates to the technical field of virus detection. The kit comprises a nucleic acid quick release reagent, a primer group, an isothermal amplification reaction system, a CRISPR (clustered regularly interspaced short palindromic repeats) detection system, a visual detection component, positive and negative controls, related buffer solutions, enzymes and the like. The detection method integrates nucleic acid extraction-free, ERA isothermal amplification and CRISPRCas12a technologies, realizes rapid amplification and specific detection of target virus nucleic acid, and supports three result interpretation modes of fluorescence, ultraviolet visualization and a lateral flow chromatography test strip. The method has high sensitivity and specificity, the whole process from sample treatment to result output can be completed within 50 min, complex instruments and nucleic acid purification steps are not needed, operation is easy and convenient, cost is low, and the method is suitable for on-site rapid screening of the macaque cytomegalovirus and basic-level facility application.

The invention relates to the technical field of medical examination, in particular to a rapid extraction-free hepatitis C virus detection kit and method. According to the invention, the material carrier is used for providing carrier support for reagent freeze-drying, and during use, the remelting buffer solution is used for remelting, so that the use effect before freeze-drying is achieved. The kit provided by the invention is used as a freeze-drying reagent, and solves the problem of cold-chain transportation of nucleic acid detection kits. The product simulates storage under different temperature conditions, detection results show that the product changes within 15 months, the storage period exceeds that of a control kit, and a new direction is laid for development and exploration of a later nucleic acid detection kit (pcr method). The kit disclosed by the invention lays a foundation for market occupation and long-term development by utilizing storage stability and transportation stability of the kit.