129 results about "Viral test" patented technology

The present invention provides a rabies virus G protein antigen epitope targeting completely human monoclonal antibody and applications thereof, the completely human monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region has three complementary determining region amino acid sequences: GDISSSCFY, IHYSGST and ARHRRGYCYDSEKGGTNWFDP, and the light chain variable region has three complementary determining region amino acid sequences: GDISSSCFY, IHYSGST and ARHRRGYCYDSEKGGTNWFDP. The amino acid sequences of the three complementary determining regions of the light chain variable region are respectively as follows: QGISND, ATS and LQDYEFPLT. The fully human monoclonal antibody has efficient and broad-spectrum anti-rabies virus neutralizing activity, is high in expression, fully human-derived and good in stability, and can be used for preparing rabies virus detection products or drugs for preventing and treating rabies.

The invention is applicable to the technical field of virus detection, and provides a norovirus detection kit based on a photo-thermal isothermal amplification technology and a detection method, the norovirus detection kit comprises a nucleic acid amplification chamber, the nucleic acid amplification chamber is provided with reaction micropores for nucleic acid amplification; an RT-PRA system, a CRISPR-cas12a system, a blank control and a positive control are placed in the reaction micropore, the RT-PRA system comprises RT-PRA solutions R1-R5, and the CRISPR-cas12a system comprises CRISPR-Cas12a solutions C1-C3; and the photo-thermal film covers the nucleic acid amplification chamber and is used for converting sunlight into heat energy and radiating the heat energy to liquid in the reaction micropores so as to provide reaction temperature for nucleic acid amplification. According to the invention, the portable, rapid and accurate detection of norovirus infection can be realized, the detection cost can be effectively reduced, the application scene is wide, workers can be helped to deduce the virus transmission path, and key support is provided for investigation and analysis of norovirus epidemic situations.

The invention provides application of high-concentration colloidal gold in preparation of an influenza virus detection test strip, and relates to the technical field of immunodetection. The detection test strip provided by the invention is composed of a sample pad, a combination pad, a nitrocellulose membrane, a water absorption pad and a bottom plate, and in the preparation process of the detection test strip, high-concentration colloidal gold with the OD value of 100 is adopted for respectively marking an influenza A virus monoclonal antibody 1, an influenza B virus monoclonal antibody 1 and a chicken IgY antibody; optimized colloidal gold labeled reconstitution fluid is mixed and then sprayed on the combination pad, and meanwhile, a corresponding capture antibody and a goat anti-chicken IgY antibody are respectively sprayed on the nitrocellulose membrane to form a detection line and a quality control line. Through the synergistic effect of the high-concentration colloidal gold mark, the optimized sample pad / combination pad treating fluid and the special sample diluent, the detection sensitivity is remarkably improved, the false negative rate is effectively reduced, and rapid, high-sensitivity and high-specificity field detection on influenza A and B viruses is realized.

The invention discloses an indirect ELISA (enzyme-linked immuno sorbent assay) detection method of a porcine epidemic diarrhea virus antibody SIgA, and belongs to the technical field of animal antibody detection. In order to solve the problems of limited detection range and single immune evaluation dimension of a porcine epidemic diarrhea virus detection method in the prior art, the invention provides an indirect ELISA detection method of a porcine epidemic diarrhea virus antibody SIgA, and the detection method adopts a pichia pastoris eukaryotic secretion platform to prepare a high-activity PEDV S1 antigen; meanwhile, a secreting type IgA antibody is used as a detection target; the method has the advantages of high detection sensitivity, low background noise, better variant compatibility and practical popularization value, and provides a clinical and industrial-level solution for maternal immunity monitoring, piglet passive protection evaluation and vaccine immunity strategy optimization.

The application discloses a reagent, a method and application of the reagent and the method for simultaneously detecting three common viruses in a fancy carp, and belongs to the technical field of fish quarantine. The application provides a reaction system and a detection method for simultaneously detecting three common viruses in a fancy carp, establishes a multiplex fluorescence PCR method, adopts a full-closed reaction, solves the problem that common PCR is prone to generating aerosols and causing false positive results of tests, has high detection sensitivity, can detect target genes with a copy number of 10 orders of magnitude at the minimum, is suitable for detecting a small amount of viruses carried in normal fancy carp or viruses in water quality environment, and can fully meet the requirements of customs quarantine prevention and control, has strong specificity, has no cross reaction to templates such as IHNV, RSIV and EHNV, and further provides a virus-like particle which can be used as a positive reference product in the multiplex system, is stable in state and beneficial to storage, can monitor an extraction process, and ensures that a test is established.

The application discloses nanobodies against E protein of chikungunya virus and application in virus detection, and the nanobodies against E protein of chikungunya virus include N055 and 10G4 antibodies, and both have high affinity and binding activity with antigens. A double antibody sandwich enzyme-linked immunosorbent assay method constructed by the nanobodies of the N055 and 10G4 antibodies can efficiently detect E protein of chikungunya virus and virus-like particles, and the detection sensitivity can reach 49 pg / mL, and the antibody combination has wide application prospects in diagnosis and pathogen detection of chikungunya virus disease.

The invention discloses primers, a kit and a method for detecting four viruses in lentinus edodes. According to the invention, LAMP (loop-mediated isothermal amplification) primer groups are respectively designed and screened according to four viruses in the lentinula edodes, and the four viruses are respectively a Lentinula edodes dominant virus 1 virus, a Lentinula edodes dominant virus HKB virus, a Lentinula edodes dominant gene-like virus 1-Hunong-1 virus and a Lentinula edodes dominant virus 5 virus. The detection system is micro-quantized, the local concentration of the target sequence is improved from the side, the detection limit is greatly improved, and the lower detection limit can reach 10 copy / microliter.

The invention discloses a Nipah virus F protein monoclonal antibody and application thereof, the antibody comprises an antibody 2D12 and an antibody 3B10, and the Nipah virus F protein monoclonal antibody is prepared from Nipah virus F protein immunogen. The specific anti-Nipah virus F protein monoclonal antibody pair is prepared and detected, the monoclonal antibody pair can be specifically combined with the Nipah virus F protein, is high in titer and good in affinity and has neutralizing activity, and meanwhile, the detection kit prepared on the basis of the antibody pair can effectively carry out Nipah virus detection; and technical support can be provided for treatment, epidemiological monitoring and diagnosis of Nipah virus infection.

The application discloses a new coronavirus strain XBB.1.5 identification method based on a surface-enhanced Raman spectrum chip, and belongs to the technical field of new coronavirus detection. The application identifies the new coronavirus strain XBB.1.5 through the detection method, avoids complex and expensive gene sequencing, can accurately determine the new coronavirus subtype, has low cost, high accuracy, short time and convenient operation.

The invention belongs to the technical field of aquatic pathogen detection, and provides a primer and probe combination with strong specificity and high sensitivity, a kit and a cdPCR detection method in order to solve the problems of dependence of absolute quantification of an existing qPCR technology on a standard curve and low repeatability and stability caused by uncontrollable quality of a standard substance. The primer probe can generate specific amplification on LYCIV and has no cross reaction on other common aquatic pathogens (such as NNV, DIV1 and the like), so that the specificity of a detection result is fundamentally ensured, a false positive result caused by the cross reaction is effectively avoided, and the diagnosis accuracy is improved. The method has the characteristics of extremely high sensitivity and absolute quantification, the lower limit of detection reaches 6.2 copies / mu L, the method has more advantages in quantification of samples with extremely low concentration, early diagnosis and detection of extremely low virus load can be realized, viruses with extremely low content in fish bodies can be detected earlier, precious time is provided for disease early warning and early intervention, and the method is worthy of popularization and application. Disease outbreak is effectively prevented.

The invention relates to the technical field of virus detection, in particular to a fluorescent RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection probe 1106VP and / or 1106WP and primers 1106VWF and 1106VWR. The invention also discloses a fluorescent RT-PCR detection reagent, a fluorescent RT-PCR detection kit, a fluorescent RT-PCR detection probe, a primer, the fluorescent RT-PCR detection reagent and the application of the fluorescent RT-PCR detection kit in identification of a porcine reproductive and respiratory syndrome NJ-1106R strain and a porcine reproductive and respiratory syndrome non-NJ-1106R strain. The probe and the primer disclosed by the invention are high in specificity and high in sensitivity; the accuracy is high, and the accuracy rate of blind sample detection is 100%; the kit is high in universality, can be used for detecting virus liquid for cell culture in a laboratory, and can also be used for detecting porcine serum and tissue samples in clinical samples. The method is an effective method for identifying the PRRSV NJ-1106R vaccine strain and other PRRSV wild strains.

The application provides a one-step RT-PCR detection kit for detecting porcine getah virus infection and application thereof, and belongs to the technical field of virus detection. The application provides a one-step RT-PCR detection kit for detecting porcine getah virus (GETV) infection and application thereof, wherein the kit contains a pair of specific primers for detecting the getah virus. The kit has good detection result specificity, and has no cross reaction with main members of pig diseases and common pig, cattle and sheep pathogens; the kit has high sensitivity, and the sensitivity for detecting plasmid standard products and GETV cell toxins is 5.9 copies and 0.3 TCID 50 respectively; the kit is rapid, sensitive, simple, low in cost, reverse transcription and PCR are performed in one reaction tube, and the pollution opportunity is reduced.

The invention relates to the technical field of biology, in particular to an enteric adenovirus hydrogel limited amplification primer combination, a kit and application of the enteric adenovirus hydrogel limited amplification primer combination. According to the present invention, the primer, the reaction reagent and the reaction system are optimized, and the hydrogel limiting amplification technology is adopted to develop the hydrogel limiting amplification reaction system suitable for enteroadenovirus detection, and the hydrogel limiting amplification reaction system is used for enteroadenovirus detection, the method is simple and rapid in steps, low in equipment requirement, capable of effectively preventing and treating aerosol pollution, good in storage stability, high in sensitivity and high in specificity.

This utility model discloses a porcine reproductive and respiratory syndrome virus (PRRSV) detection kit, relating to the field of detection kits. The PRRSV detection kit includes a kit body, a bracket at the bottom of the kit body, and a detection mechanism on the outside of the kit body. The detection mechanism includes a detection block disposed inside the kit body, a sealing block movably disposed above the detection block, and a fixing bracket movably engaged inside the bracket and movably disposed above the detection block, thus fixing the kit body and the bracket in place. This PRRSV detection kit allows for the determination of the injection site of the kit body without opening the kit body using the detection block. Subsequently, the sealing block sequentially seals the area above the kit body, and the moving fixing bracket secures all kit bodies above the bracket, reducing the complexity of this fixation method and ensuring that the use of the sealing block does not affect the overall fixation effect of the kit body and bracket.

The application discloses a nano antibody against Ebola virus VP40 protein and application thereof in Ebola virus detection, wherein the nano antibody against Ebola virus VP40 protein is a 3E07 antibody. The nano antibody against Ebola virus VP40 protein prepared by the application has the characteristics of high sensitivity and wide binding spectrum. A double antibody sandwich enzyme-linked immunosorbent assay method constructed by 3E07 and 3F06 antibodies can efficiently detect VP40 proteins and virus-like particles of various Ebola virus subtypes such as Zaire, Sudan and Beni, wherein the detection sensitivity of the Ebola virus VP40 protein of Zaire type can reach 0.039 ng / mL, and the antibody combination has a wide application prospect in the diagnosis and pathogen detection of Ebola virus disease.

This invention relates to an ELISA detection kit for porcine reproductive and respiratory syndrome virus (PRRSV) based on M-GP5 recombinant protein, and belongs to the field of ELISA detection technology. The kit includes recombinant (M-GP5) antigen protein, an ELISA plate, blocking buffer, primary antibody dilution buffer, enzyme-labeled secondary antibody, secondary antibody dilution buffer, chromogenic solution, stop solution, negative serum, and positive serum. It features high sensitivity, high specificity, and good stability, and can accurately detect PRSV antibodies.

The invention discloses a macaque cytomegalovirus detection kit supporting multi-mode interpretation and a detection method, and particularly relates to the technical field of virus detection. The kit comprises a nucleic acid quick release reagent, a primer group, an isothermal amplification reaction system, a CRISPR (clustered regularly interspaced short palindromic repeats) detection system, a visual detection component, positive and negative controls, related buffer solutions, enzymes and the like. The detection method integrates nucleic acid extraction-free, ERA isothermal amplification and CRISPRCas12a technologies, realizes rapid amplification and specific detection of target virus nucleic acid, and supports three result interpretation modes of fluorescence, ultraviolet visualization and a lateral flow chromatography test strip. The method has high sensitivity and specificity, the whole process from sample treatment to result output can be completed within 50 min, complex instruments and nucleic acid purification steps are not needed, operation is easy and convenient, cost is low, and the method is suitable for on-site rapid screening of the macaque cytomegalovirus and basic-level facility application.

The application provides a porcine parvovirus detection method and kit based on the nature of four-strand DNA oxidoreductase. The method uses isothermal amplification technology to amplify the genomic fragment containing the four-strand DNA sequence in the porcine parvovirus genome, and realizes rapid and visual detection of porcine parvovirus by adding hematin and oxidoreductase substrate for color reaction.

The application provides a composition, a kit and a method for human cytomegalovirus detection, and relates to the technical field of molecular diagnosis.The composition comprises at least one set of human cytomegalovirus detection primer probe groups and multiplex DNA polymerase, wherein the primer probe groups comprise oligonucleotide upstream external primers, downstream external primers, upstream internal primers, downstream internal primers, upstream loop primers and downstream loop primers; the probe is a neck ring structure probe, and the multiplex DNA polymerase comprises strand displacement DNA polymerase and DNA polymerase with 5' end to 3' end exonuclease activity.The composition for human cytomegalovirus detection alleviates the technical problems that the LAMP amplification scheme in the prior art is poor in specificity and cannot realize single-tube multiplex detection.

The application belongs to the technical field of virus detection, and discloses a sheep disease virus multiplex PCR detection primer, method and application. The base sequence of the primer FMDV-F of the sheep foot-and-mouth disease virus is SEQ ID NO:1, the base sequence of the primer FMDV-R is SEQ ID NO:2; the base sequence of the primer ORFV-F of the sheep orf virus is SEQ ID NO:3, the base sequence of the primer ORFV-R is SEQ ID NO:4; the base sequence of the primer GTPV-F of the goat pox virus is SEQ ID NO:5, and the base sequence of the primer GTPV-R is SEQ ID NO:6. The application optimizes the multiplex PCR, determines the optimal reaction system and the optimal reaction procedure, and can accurately detect single or mixed infection of the sheep foot-and-mouth disease virus, the sheep orf virus and the goat pox virus, and has an important role in epidemiological investigation and epidemic disease prevention and control.

The invention relates to ferric hydroxide modified diatomite, a preparation method and application in ASFV detection, and belongs to the technical field of virus detection. The invention provides a preparation method of ferric hydroxide modified diatomite. The preparation method comprises the following steps: (1) dropwise adding a saturated FeCl3 solution into water, and stirring to obtain Fe (OH) 3 colloid; and (2) mixing the Fe (OH) 3 colloid obtained in the step (1) with diatomite, and drying to obtain the ferric hydroxide modified diatomite. The ferric hydroxide modified diatomite prepared according to the method has a good concentration effect on ASFV, the ferric hydroxide modified diatomite is adopted to concentrate a sample which is extremely low in ASFV pathogen content and is negative through conventional qPCR detection, the amplification efficiency of the detected sample can be improved, the CT value of the sample is reduced by 1.5-3, and the detection sensitivity is improved. The sensitivity and the accuracy of sample detection are improved.

This invention relates to the field of biotechnology, specifically disclosing a Decacovirus nucleic acid detection composition based on RT-RPA and CRISPR / Cas13a technologies and its applications. This invention successfully obtains a novel method for Decacovirus nucleic acid detection based on RT-RPA and CRISPR / Cas13a technologies. The nucleic acid detection composition includes: an RT-RPA primer pair and crRNA; the RT-RPA primer pair consists of two RT-RPA primers, with sequences shown in SEQ ID NO.1 and SEQ ID NO.2; the crRNA sequence consists of an anchoring sequence for binding to the Cas13a protein and a guide sequence targeting the Decacovirus gene target sequence, with the guide sequence shown in positions 23-50 of SEQ ID NO.3. Using this composition for Decacovirus detection offers advantages such as high specificity, high sensitivity, rapid and convenient operation, solving the problem of inconvenience in current field monitoring of the virus's prevalence.

A real-time, in situ and automated technology and method for dynamic observation of airborne viruses has been developed, and its application for airborne viruses, using a model virus, MS2 bacteriophage, and SARS-COV-1 RNA, has been demonstrated. There is described a method of performing measurements using a digital in-line holographic microscope (DIHM). The method generally has: propagating a light beam through a pinhole, across a medium including particles, and to a sensor; acquiring, with the sensor, a plurality of holograms, the holograms including scattering information of the particles; and determining, from a reconstruction of the holograms, at least one of shape, size, intensity and phase of the particles from the scattering information of said particles.

In the field of Hepatitis B virus (HBV) detection, disclosed are a method for detecting HBcAg by means of using a double antibody sandwich method, and an antibody and kit for detecting HBcAg; also included is a monoclonal antibody that can be used in the immunological detection of HBcAg in a tissue or cell sample.

A kind of MOFs nano-enzyme and application in new coronavirus detection, it relates to biological detection method field, MOFs-based nano-enzyme has peroxidase-like effect, can catalyze color developing agent discoloration;After the specific protein CD147 is modified on the surface of nano-enzyme, using the specific binding of CD147 and the S protein overexpressed on the surface of new coronavirus, new coronavirus can be efficiently combined on the surface of nano-enzyme to inhibit the catalytic color development of nano-enzyme;Based on the principle, the quantitative and qualitative detection of new coronavirus can be realized by colorimetric method;The present application realizes the rapid, high-sensitivity and visual detection of new coronavirus by the inherent catalytic advantage of nano-enzyme and the specific recognition and binding capacity of CD147 to new coronavirus without additional labeling process, and can effectively identify new coronavirus infection.

The invention discloses a DNA (Deoxyribonucleic Acid) molecule, an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) primer group and application of the RT-PCR primer group in tomato ringspot virus detection. Belongs to the technical field of plant quarantine. A gene segment SEQ ID NO.1 with a high conservative characteristic is selected from RNA1 of ToRSV, the gene segment is used as a target gene, a corresponding amplification primer is designed to detect the tomato ringspot virus, an optimal reaction condition is researched through a series of condition exploration and optimization, the detection sensitivity and specificity are greatly improved, and the detection result is more accurate. The detection period is shortened, the detection process is accelerated, technical support is provided for shortening the detection period of an inspection and quarantine department, and a foundation is laid for accurately and rapidly identifying pathogens.

The invention provides a broad-spectrum anti-rabies virus fully-humanized monoclonal antibody and application thereof, the fully-humanized monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region has three complementary determining region amino acid sequences: GFTFSDYE, ISSSGATK and ARVPGQQVGVDR, and the light chain variable region has three complementary determining region amino acid sequences: GFTFSDYE, ISSSGATK and ARVPGQQVGVDR. The amino acid sequences of three complementary determining regions of the light chain variable region are RAIHNY, ATS and QQYYGYPWT respectively. The fully human monoclonal antibody R06 has efficient and broad-spectrum anti-rabies virus neutralizing activity, is high in expression, fully human-derived and good in stability, and can be used for preparing rabies virus detection products or drugs for preventing and treating rabies.

The invention belongs to the technical field of virus detection, and particularly relates to a porcine delta coronavirus N protein monoclonal antibody and a latex immunochromatography test strip containing the antibody. According to the invention, a prokaryotically expressed PDCoV N protein is used as an immunogen to immunize a mouse, and a specific monoclonal antibody 2G3 and a specific monoclonal antibody 9C4 are screened through cell fusion and a subcloning technology. The test strip comprises a PVC (polyvinyl chloride) bottom plate, a sample pad, a combination pad, a nitrocellulose membrane (NC membrane) and a water absorption pad, a quality control line C and a detection line T are respectively arranged on the NC membrane. The test strip can specifically recognize PDCoV, the lowest detection limit can reach 103.0 TCID50 / mL, the test strip has no cross reaction with other clinical common diarrhea viruses, the result interpretation only needs 15 min, and the test strip has the advantages of being rapid, sensitive, easy and convenient to operate and the like.

The present invention relates to a biological detection chip for African swine fever virus and its application thereof. The biological detection chip comprises a plurality of transistors connected in parallel, each of which individually includes a substrate layer, a floating gate, a metal connecting channel, an extending gate, and a biological detection layer. The biological detection chip is functionalized through surface modification process to integrate biological probes, enabling the detection of African swine fever virus in test samples.

The invention discloses a primer group and a kit for simultaneously detecting a panda aleutian virus and a panda canine distemper virus Asia type 6 and application of the primer group and the kit, and belongs to the technical field of virus detection of endangered wild animals. The invention provides a primer group and a kit for specifically recognizing the aleutian disease virus and the canine distemper virus Asia 6 type of the panda, and detection can be completed within 20 minutes under the condition of constant temperature of 39 DEG C; according to the present invention, the method has characteristics of rapid detection, good specificity, high sensitivity and the like, does not produce the cross reaction with other common pathogens, does not produce the false positive phenomenon due to the generation of the primer dimer, has characteristics of rapid detection, good specificity, high sensitivity and the like, and has characteristics of simple and easy operation, and on-site detection without special detection personnel; the method is rapid and accurate in detection, has a good application effect in preliminary application, and provides a method for rapid diagnosis, virus traceability, epidemic disease monitoring and early warning of a wild animal epidemic disease source and the like in zoos and wild animal rescue institutions.