432 results about "Viral test" patented technology

The invention discloses a hybridoma cell strain, a monoclonal antibody, a linear epitope antigen and application thereof, and relates to the field of genetic engineering, in particular to a hybridoma cell strain, a monoclonal antibody, a linear epitope antigen and application thereof. The preservation number of the hybridoma cell strain P27-A1 is CGMCC (China General Microbiological Culture Collection Center) No. 46352. The hybridoma cell strain P27-A1 is prepared from feline leukemia virus p27, and a monoclonal antibody secreted by the hybridoma cell strain P27-A1 recognizes a linear epitope antigen of feline leukemia virus p27 protein; the kit for the feline leukemia virus p27 comprises the monoclonal antibody A1 secreted by the hybridoma cell strain P27-A1. The monoclonal antibody secreted by the hybridoma cell strain can greatly improve the sensitivity of the p27 protein for detecting feline leukemia virus. The invention provides the monoclonal antibody secreted by the hybridoma cell strain for identification, and the monoclonal antibody can be used for preparing a diagnostic kit such as a colloidal gold test strip.

The invention belongs to the field of veterinary biotechnology detection, and particularly relates to a hybridoma cell strain and a monoclonal antibody for detecting or identifying MDV-1 and application of the hybridoma cell strain and the monoclonal antibody. The hybridoma cell strain MDV-1Mab-pp38-E2 is obtained through screening by taking pp38 protein of MDV-1 as immunogen, the preservation number is CCTCC NO: C202501, the monoclonal antibody generated by the hybridoma cell strain can recognize I-type chicken Marek's disease virus strains with different serum, and the monoclonal antibody can be used for identifying the type I chicken Marek's disease virus strains with different serum types. The kit has good specificity and sensitivity and can be used for exogenous virus detection of the serum type I chicken Marek's disease virus in poultry virus live vaccines without cross reaction with serum type II chicken Marek's disease virus, serum type III chicken Marek's disease virus, chicken egg drop syndrome virus (EDSV) and other common poultry disease viruses, and the kit has good specificity and sensitivity and can be applied to exogenous virus detection of the serum type I chicken Marek's disease virus in poultry virus live vaccines. The kit can also be used for clinical identification, virus content determination and epidemiological investigation of serotype I chicken Marek's disease virus.

The invention relates to the technical field of human papilloma virus detection, in particular to a primer probe combination, a reagent or kit for human papilloma virus nucleic acid detection and application. The purpose of detecting different types is achieved by adding different specific probes, 18 common human papilloma virus DNAs which are internationally recognized to be closely related to cervical cancer can be detected at a time, and HPV16 and HPV18 types are subjected to typing detection. The application provides 6 universal primers (including 4 target primers and 2 internal standard primers) and 19 specific probes (including 18 target probes and 1 internal standard probe), the 4 target primers can amplify DNA of 18 human papilloma viruses, and the 18 probes are specific probes designed for the 18 human papilloma viruses. And meanwhile, by optimizing the primer probe and the reaction liquid, the purpose of detection is achieved.

The invention relates to a Nipah virus N protein specific monoclonal antibody as well as a preparation method and application thereof. The nipah virus specific monoclonal antibody is prepared by immunizing nipah virus N protein, the amino acid sequence of the nipah virus N protein is as shown in SEQ ID NO.2, the nipah virus N protein is obtained by transcription of a nipah virus N gene, and the nucleotide sequence of the nipah virus N gene is as shown in SEQ ID NO.1. The nipah virus specific monoclonal antibody has the advantages that the nipah virus specific monoclonal antibody is prepared by immunizing the nipah virus N protein; the monoclonal antibody with the nipah virus N protein specificity can be specifically combined with the nipah virus N protein and is high in titer and good in affinity, meanwhile, a detection kit prepared based on the antibody can effectively carry out nipah virus detection, and technical support can be provided for epidemiological monitoring and diagnosis of nipah virus infection.

The present invention belongs to the field of virus detection technology, and specifically relates to monoclonal antibodies with neutralizing activity against adenovirus type 5 and their uses. The monoclonal antibody 8B9 of the present invention can specifically recognize and neutralize adenovirus type 5, and the amino acid sequences of the heavy and light chain variable regions and their complementarity determining regions of the monoclonal antibody 8B9 are provided. The present invention also provides an in vitro neutralization activity assessment model based on HEK-293A cells. At a concentration as low as 3.3 μg / mL, the titer of the neutralizing activity against the virus stock solution after 6000-fold dilution is 7×10 4 TCID 50 / mL of virus has close to 100% neutralizing activity. After further humanization, it is expected to be developed into a neutralizing antibody drug for the treatment of diseases such as severe pneumonia caused by HADV-5 infection, filling the current lack of effective treatment options in this field and possessing significant scientific research value and clinical application prospects.

The invention relates to the technical field of biological detection, in particular to a detection primer for African horse pestivirus and west nile virus and a nucleic acid mass spectrometry detection method. On the basis of an MALDI-TOF nucleic acid mass spectrum detection method, two relatively conservative genes of VP7 and NS2 are selected as target genes for the African horse pestivirus; and for the West Nile virus, two same conservative genes NS2A and NS5 are selected. Based on the target genes, four pairs of amplification primers and four extension primers are designed. By optimizing selection of amplification sites and adding of modified bases, it is ensured that the molecular weight of the extension probe is effectively distinguished within the detection range, so that the multiple detection target is achieved, and meanwhile high accuracy, specificity and sensitivity of detection are ensured. The kit can efficiently, sensitively and accurately identify the infection conditions of the African horse pestivirus and the west nile virus, can be flexibly adjusted and optimized according to actual requirements, and is expected to play an important role in virus detection and differential diagnosis in the future.

The invention provides monoclonal antibodies of H protein and N protein of canine distemper virus and application thereof in preparation of a fluorescent microsphere antigen detection test strip. The monoclonal antibody disclosed by the invention is characterized in that (1) the monoclonal antibody contains a heavy chain variable region CDV-N-3A11-VH and a light chain variable region CDV-N-3A11-VL; the amino acid sequence of the CDV-N-3A11-VH is as shown in the 1st to 116th sites of SEQ ID No.1 in a sequence table; the amino acid sequence of the CDV-N-3A11-VL is as shown in the 1st site to the 113th site of SEQ ID No.2 in a sequence table. (2) a heavy chain variable region CDV-H-1G5-VH and a light chain variable region CDV-H-1G5-VL are contained; the amino acid sequence of the CDV-H-1G5-VH is as shown in the first site to the 114 site of SEQ ID No.3 in a sequence table; the amino acid sequence of the CDV-H-1G5-VL is as shown in the 1st site to the 1st site and the 1st site to the 1st site of SEQ ID No.4 in a sequence table. The canine distemper virus fluorescent microsphere antigen test strip composed of the two antibodies is suitable for detecting canine distemper viruses in samples such as eye and nose swabs and virus cultures, is high in sensitivity and good in specificity, and can be used for early diagnosis of canine distemper virus infection.

The present invention belongs to the field of virus detection technology, and specifically relates to a combination and application of monoclonal antibodies for the specific detection of type 5 adenovirus. The antibody combination includes 8B9 and 4A6, which target different epitopes of the HADV‑5 Hexon protein, respectively, and are suitable for a double-antibody sandwich ELISA detection system. Among them, 8B9 is used as a coating antibody, and 4A6 is used as an HRP-labeled detection antibody. The combination of the two can achieve high sensitivity and high specificity detection of HADV‑5 and replication-deficient recombinant type 5 adenovirus, and does not cross-react with other common adenovirus types. The present invention also provides the variable region amino acid sequence and nucleotide sequence of the above-mentioned antibody, as well as an ELISA detection method based on the antibody combination. The method is simple to operate, has good repeatability, and is suitable for the detection of type 5 adenovirus.

The invention belongs to the technical field of virus detection, and discloses a digital PCR (Polymerase Chain Reaction) kit for simultaneously detecting African horse pestivirus, West Nile virus and equine influenza H3N8, and the kit comprises primers and probes as shown in SEQ ID NO.1-SEQ ID NO.21. The invention also discloses a kit for simultaneously detecting African horse pestivirus, West Nile virus and equine influenza H3N8. According to the invention, after a single system of an African horse pestivirus VP7 gene, an African horse pestivirus-NS2 gene, a west nile virus-NS5 gene, a west nile virus-NS2a gene, an equine influenza H3N8-HA gene and an equine influenza H3N8-NA gene is tested, a seven-fold digital PCR (Polymerase Chain Reaction) system containing an internal standard is established; through tests of indexes such as linearity, specificity, minimum detection limit, repeatability, comparison of a single digital PCR system and a multiple digital PCR system, the currently developed digital PCR multiple detection kit completely meets development requirements and is high in specificity and sensitivity, and the minimum detection limit of the digital PCR multiple detection kit can reach 10 Copies / mu L or below.

The invention discloses an anti-Ebola virus VP40 protein nano antibody and an application of the anti-Ebola virus VP40 protein nano antibody in Ebola virus detection. The anti-Ebola virus VP40 protein nano antibody comprises a 3E07 antibody and a 3F06 antibody. The anti-Ebola virus VP40 protein nano antibody prepared by the invention has the characteristics of high sensitivity, wide binding spectrum and the like. A double-antibody sandwich enzyme-linked immunosorbent assay method constructed by the 3E07 and 3F06 antibodies can be used for efficiently detecting VP40 proteins and virus-like particles of various subtype Ebola viruses such as Zaire, Sudan, Bandong and the like, and the detection sensitivity for the VP40 proteins of the Zaire type Ebola viruses can reach 0.039 ng / mL; the antibody combination has a wide application prospect in diagnosis and pathogen detection of Ebola virus diseases.

The present invention provides a rabies virus G protein antigen epitope targeting completely human monoclonal antibody and applications thereof, the completely human monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region has three complementary determining region amino acid sequences: GDISSSCFY, IHYSGST and ARHRRGYCYDSEKGGTNWFDP, and the light chain variable region has three complementary determining region amino acid sequences: GDISSSCFY, IHYSGST and ARHRRGYCYDSEKGGTNWFDP. The amino acid sequences of the three complementary determining regions of the light chain variable region are respectively as follows: QGISND, ATS and LQDYEFPLT. The fully human monoclonal antibody has efficient and broad-spectrum anti-rabies virus neutralizing activity, is high in expression, fully human-derived and good in stability, and can be used for preparing rabies virus detection products or drugs for preventing and treating rabies.

The invention provides a characteristic protein of serotype I chicken Marek's disease virus and detection application, and belongs to the technical field of virus detection. The invention provides a recombinant gene derived from serum I type chicken Marek's disease virus. The nucleotide sequence of the recombinant gene is shown as SEQ ID No. 1. The recombinant gene disclosed by the invention is used for coding the MDV-1 recombinant PP38 protein, and the MDV-1 recombinant PP38 protein can be applied to enzyme-linked immuno sorbent assay (ELISA) detection. The invention also provides an ELISA detection kit constructed by using the MDV-1 recombinant PP38 protein, and the ELISA detection kit can be used for clinical MDV-1 serum antibody detection, SPF chicken screening, exogenous virus antibody detection of poultry live vaccines or virus seeds, epidemiological investigation and the like, so as to prevent and control transmission of MDV-1 infection.

The invention provides a hybridoma cell strain secreting a grass carp reovirus VP4 protein monoclonal antibody as well as the monoclonal antibody and application thereof, and belongs to the technical field of aquatic reovirus detection. The hybridoma cell strain is named as GCRV-VP4-6E7B12, and is preserved in the China Center for Type Culture Collection on December 10, 2024, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: C2024160. The grass carp reovirus VP4 protein monoclonal antibody 6E7B12 secreted by the hybridoma cell strain GCRV-VP4-6E7B12 has the advantage of being specifically combined with an antigen. The enzyme-labeled monoclonal antibody is used as a detection antibody, the VP4 protein polyclonal antibody is used as a capture antibody, the content of the grass carp reovirus VP4 protein in a sample to be detected can be quantitatively detected by adopting a double-antibody sandwich ELISA method, the detection range is 1-32 ng / mL, and the lower detection limit is 1 ng / mL. The detection method has the advantages of high sensitivity and wide detection range.

The invention discloses a TaqMan real-time fluorescent quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection fluorescent probe and kit for corn necrosis stripe virus and application, and relates to the technical field of virus detection. The nucleotide sequence of the fluorescent probe provided by the invention is 5 '-FAM-CGCTGGTACATAGGATGGCAAC-TAMRA-3', and the nucleotide sequence of the fluorescent probe provided by the invention is shown in the description. The nucleotide sequence of the primer is as follows: an upstream primer: 5 '-CCTGCTCCTCTGCTATTCG-3'; a downstream primer: 5 '- The downstream primer is 5 '-CAGACTTCCCTTGGCAGTGT-3', and the downstream primer is 5 '- The kit at least comprises the fluorescent probe and the primer. The TaqMan real-time fluorescent quantitative RT-PCR detection method established by the invention is simple and convenient to operate, strong in specificity, high in sensitivity and good in repeatability, is not only suitable for qualitative identification, but also can realize accurate quantitative analysis; the sensitivity is at least 100 times higher than that of a common RT-PCR detection method. The problem that in the prior art, a TaqMan real-time fluorescent quantitative RT-PCR detection method for detecting the corn necrotic stripe virus is lacked can be solved.

The invention is applicable to the technical field of virus detection, and provides a norovirus detection kit based on a photo-thermal isothermal amplification technology and a detection method, the norovirus detection kit comprises a nucleic acid amplification chamber, the nucleic acid amplification chamber is provided with reaction micropores for nucleic acid amplification; an RT-PRA system, a CRISPR-cas12a system, a blank control and a positive control are placed in the reaction micropore, the RT-PRA system comprises RT-PRA solutions R1-R5, and the CRISPR-cas12a system comprises CRISPR-Cas12a solutions C1-C3; and the photo-thermal film covers the nucleic acid amplification chamber and is used for converting sunlight into heat energy and radiating the heat energy to liquid in the reaction micropores so as to provide reaction temperature for nucleic acid amplification. According to the invention, the portable, rapid and accurate detection of norovirus infection can be realized, the detection cost can be effectively reduced, the application scene is wide, workers can be helped to deduce the virus transmission path, and key support is provided for investigation and analysis of norovirus epidemic situations.

The invention belongs to the technical field of biology, and particularly relates to a real-time fluorescent quantitative PCR (polymerase chain reaction) detection kit for porcine reproductive and respiratory syndrome virus and application of the real-time fluorescent quantitative PCR detection kit. The invention designs a primer probe combination for detecting porcine reproductive and respiratory syndrome virus, the primer probe combination consists of a primer P1, a primer P2 and a probe, and the sequences of the primer P1, the primer P2 and the probe are respectively shown as a sequence 1, a sequence 2 and a sequence 3. The invention further provides a kit containing the primer probe combination. The kit can be used for identifying and detecting the porcine reproductive and respiratory syndrome virus. The primer P1 and the primer P2 designed by the invention have strong specificity and high sensitivity, the detection sensitivity of the primer P1 and the primer P2 to the porcine reproductive and respiratory syndrome virus reaches 1.0 * 10 < 1 > copies / mu L, the detection repeatability is good, and the primer P1 and the primer P2 can be used for rapidly detecting the porcine reproductive and respiratory syndrome virus.

The invention belongs to the field of in-vitro diagnosis and detection, and particularly relates to a primer pair, a probe, a kit and a detection method for norovirus detection. The norovirus detection primer pair provided by the invention comprises a primer pair for detecting a norovirus GI type, a primer pair for detecting a norovirus GII type and a primer pair for detecting a norovirus GIV type, and specific primers and probes are designed on the basis of a norovirus GI / GII / GIV type gene conserved region; and a plurality of pairs of primers or probes are introduced, and degenerate primers and degenerate probes are introduced, so that the existing norovirus subtypes are better matched, the detection coverage rate is increased, and the omission ratio is reduced.

The invention provides a loop-mediated amplification primer group, a kit and a detection method for detecting influenza A virus, influenza B virus and respiratory syncytial virus, and relates to the technical field of biotechnology. Specific loop-mediated amplification primers are respectively designed aiming at M1 protein genes of influenza A viruses, NEP genes of influenza B viruses, F gene conserved segments of respiratory syncytial viruses and human RnaseP genes, and each set of primers comprises two outer primers (OF / OB), two inner primers (FIP / BIP) and at least one loop primer (LF / LB). Compared with a traditional detection method, the detection time is greatly shortened, a basis can be provided for clinical diagnosis more quickly, treatment measures can be taken in time, complex nucleic acid extraction and purification steps are not needed, the swab sample can be detected after being simply treated, the operation difficulty and the requirement for professional skills of operators are reduced, and the detection efficiency is improved. And popularization and application in all levels of medical institutions are facilitated.

The invention relates to the technical field of virus detection, in particular to a primer probe combination for bovine respiratory disease syndrome pathogen identification and application and a kit thereof. When the bovine viral diarrhea virus is identified, the nucleotide sequence of the upstream primer of the used primer probe group is as shown in SEQ ID No.1, the nucleotide sequence of the downstream primer is as shown in SEQ ID No.2, and the nucleotide sequence of the probe is as shown in SEQ ID No.3. The primer probe combination provided by the invention can be used for identifying and diagnosing eight common pathogens capable of causing the bovine respiratory disease syndrome at one time through a two-tube reaction system by using a fluorescent quantitative PCR method, can also be used for identifying a mixed infected sample, has the advantages of accurate identification, high sensitivity, simplicity in operation, short time consumption and the like, and can be used for detecting the bovine respiratory disease syndrome. And an effective technical means can be provided for pathogen diagnosis and disease prevention of the bovine respiratory disease syndrome in China.

The invention provides a primer group, a kit and a method for targeted amplification of a type I dengue virus genome, and application of the primer group, the kit and the method, relates to dengue virus detection, and belongs to the technical field of genome detection. The universal primer group for amplifying the I-type dengue virus genome is designed, targeted amplification can be performed on the I-type dengue virus genome, sequencing and sequence splicing are performed by utilizing a three-generation sequencing platform after a library is constructed, and the I-type dengue virus genome is obtained. The method provided by the invention can amplify all I-type dengue virus subtypes, and has the advantages of large flux, low cost and simple sequence splicing.

The invention provides a reagent for detecting avian leukosis virus, a nano-enzyme immunochromatography test strip and application of the nano-enzyme immunochromatography test strip, and belongs to the technical field of immunology. Aiming at the problems that the existing avian leukosis virus detection method is complicated in operation, time-consuming or strong in equipment dependence, the reagent comprises a capture antibody and a detection antibody; a heavy chain variable region of the monoclonal antibody A comprises an amino acid sequence as shown in SEQ ID NO: 1, and a light chain variable region of the monoclonal antibody A comprises an amino acid sequence as shown in SEQ ID NO: 2; a heavy chain variable region of the monoclonal antibody B comprises an amino acid sequence as shown in SEQ ID NO: 5, and a light chain variable region of the monoclonal antibody B comprises an amino acid sequence as shown in SEQ ID NO: 6; the antigen binding fragment is a Fab fragment, an F (ab) 2 fragment or a single-chain Fv fragment. The reagent is mainly used for detecting avian leukosis virus, and is suitable for detecting samples such as cloaca swab, meconium, egg white or serum of poultry.

The invention discloses an efficient acclimatization and transplantation method for sweet potato virus-free test-tube plantlets, which comprises the following steps: removing fibrous roots from sweet potato virus-free test-tube plantlets which are subcultured for 20-30 days through virus detection, treating the sweet potato virus-free test-tube plantlets for 24 hours through an acclimatization nutrient solution, directly planting the sweet potato virus-free test-tube plantlets into a transplantation matrix, keeping the temperature of a culture area at 28 + / -2 DEG C, keeping the relative humidity at 75-80%, and culturing for 3 days under weak light, and supplementing carbon dioxide to the culture area until the concentration is 1000-1200ppm, culturing for 20-30 days under normal illumination, transferring the sweet potato virus-free test-tube plantlet with the survival zone transplanting matrix into a hole tray, filling a seedling culture matrix to ensure that each hole of the matrix is full, watering, carrying out shading treatment for 3-5 days, and transferring to a normal illumination condition for culturing, so as to obtain the sweet potato virus-free test-tube plantlet. According to the method, the seedling age of virus-free seedling transplanting is reduced to 20-30 days, the cost is saved, the transplanting survival rate is remarkably increased, the key technical obstacle from test-tube plantlets to propagation seedlings is broken through, and the sweet potato virus-free test-tube plantlets can be quickly applied to production.

The invention provides application of high-concentration colloidal gold in preparation of an influenza virus detection test strip, and relates to the technical field of immunodetection. The detection test strip provided by the invention is composed of a sample pad, a combination pad, a nitrocellulose membrane, a water absorption pad and a bottom plate, and in the preparation process of the detection test strip, high-concentration colloidal gold with the OD value of 100 is adopted for respectively marking an influenza A virus monoclonal antibody 1, an influenza B virus monoclonal antibody 1 and a chicken IgY antibody; optimized colloidal gold labeled reconstitution fluid is mixed and then sprayed on the combination pad, and meanwhile, a corresponding capture antibody and a goat anti-chicken IgY antibody are respectively sprayed on the nitrocellulose membrane to form a detection line and a quality control line. Through the synergistic effect of the high-concentration colloidal gold mark, the optimized sample pad / combination pad treating fluid and the special sample diluent, the detection sensitivity is remarkably improved, the false negative rate is effectively reduced, and rapid, high-sensitivity and high-specificity field detection on influenza A and B viruses is realized.

The invention discloses a peste des petits ruminants virus specific monoclonal antibody and application thereof.The monoclonal antibody is obtained by screening peste des petits ruminants virus N protein, the amino acid sequence of the peste des petits ruminants virus N protein is shown as SEQ ID NO: 1, the peste des petits ruminants virus N protein is obtained by transcription of a peste des petits ruminants virus N gene, and the peste des petits ruminants virus N gene is shown as SEQ ID NO: 2. The nucleotide sequence of the codon-optimized peste des petits ruminants virus N gene is shown as SEQ ID NO: 2. The monoclonal antibody provided by the invention can be specifically combined with the peste des petits ruminants virus N protein, the titer is high, the affinity is good, meanwhile, the detection kit prepared based on the antibody pair can effectively carry out peste des petits ruminants virus detection, and a technical support can be provided for epidemiological monitoring and diagnosis of peste des petits ruminants virus infection.

The invention discloses an indirect ELISA (enzyme-linked immuno sorbent assay) detection method of a porcine epidemic diarrhea virus antibody SIgA, and belongs to the technical field of animal antibody detection. In order to solve the problems of limited detection range and single immune evaluation dimension of a porcine epidemic diarrhea virus detection method in the prior art, the invention provides an indirect ELISA detection method of a porcine epidemic diarrhea virus antibody SIgA, and the detection method adopts a pichia pastoris eukaryotic secretion platform to prepare a high-activity PEDV S1 antigen; meanwhile, a secreting type IgA antibody is used as a detection target; the method has the advantages of high detection sensitivity, low background noise, better variant compatibility and practical popularization value, and provides a clinical and industrial-level solution for maternal immunity monitoring, piglet passive protection evaluation and vaccine immunity strategy optimization.

The invention belongs to the field of canine distemper virus detection, and particularly relates to a canine distemper virus RT-RAA CRISPR / Cas 12b detection kit and a detection method thereof. According to the kit, isothermal amplification is performed by using an RT-RAA universal primer, and then detection is performed by using a CRISPR / Cas 12b detection system consisting of target sequence sgRNA, so that amplification of a target fragment of the N gene of the canine distemper virus and target detection visualization can be effectively realized. The result of the embodiment shows that the RT-RAA CRISPR / Cas 12b detection kit finally designed by the invention is high in specificity and does not have cross reaction with other viruses, the lowest visual detection limit reaches 4.77 * 10 < 1 > copies / mu L, the virus can be instantly detected when the RT-RAA CRISPR / Cas 12b detection kit is used for detecting the CDV, the labor and equipment cost is low, the period is short, and the detection time does not exceed 2 hours. The rapid detection technology can be popularized and applied to epidemiological investigation and epidemic situation monitoring of the canine distemper virus, is suitable for epidemic disease monitoring of wild animals, quarantine of zoos and on-site rapid detection of clinical samples of canines, and has good practical significance and wide market prospects.

The invention provides a kit and a method for detecting human adenoviruses 3 and 7, and belongs to the technical field of virus detection. According to the present invention, the MCDA amplification technology and the CRISPR / Cas12b detection technology are combined, and the MCDA amplification primer, the gRNA and the probe molecule are designed according to the Hexon gene of the HAdv-3 and the HAdv-7; the kit and the detection method provided by the invention have the advantages of high sensitivity, good specificity, high detection efficiency, accurate and objective interpretation and good repeatability, and can realize accurate detection of HAdv-3 and HAdv-7; and the detection method has low requirements on equipment, is less limited by places, is simple and convenient to operate, and can realize on-site and real-time detection of HAdv-3 and HAdv-7.

The present invention relates to a kit for detecting porcine epidemic diarrhea and a preparation method thereof, belonging to the technical field of virus detection, and specifically comprising the following steps: after extracting the antigen, treating it with a virus purification agent containing polyethylene glycol, nuclease and concanavalin, and then obtaining a virus antigen suspension with a composite dissociation solution. The suspension is coated on a microporous reaction plate to form a solid-phase antigen. Subsequently, the porcine epidemic diarrhea virus solid-phase antigen, enzyme marker, sample diluent, Tris buffer, color developer and stop solution are aseptically stored to prepare a kit for detecting porcine epidemic diarrhea. The present invention provides a novel kit and a preparation method thereof, which can not only effectively purify porcine epidemic diarrhea virus, but also significantly reduce the risk of protein precipitation, while overcoming nucleic acid interference and improving purification efficiency.

The invention belongs to the technical field of African swine fever virus detection, and particularly relates to an RPA primer for detecting African swine fever virus, a CRISPR / Cas12 system target, a kit and application. The method comprises the following steps: pre-treating a to-be-detected sample by using a nucleic acid rapid releasing agent; by designing a CRISPR / Cas suboptimal system and screening a target sequence, an RPA (recombinase polymerase amplification) technology is combined with the CRISPR / Cas suboptimal system, and a novel method for rapidly detecting the nucleic acid of the African swine fever virus by a one-tube method is developed. The method does not depend on professional laboratories, instruments and equipment, nucleic acid in a sample is subjected to extraction-free treatment by using a nucleic acid rapid releasing agent, the African swine fever virus nucleic acid is rapidly detected by using a one-tube method, a result is directly observed by naked eyes, and the limitation and the defects of application scenes of traditional ASFV nucleic acid detection are solved.

The invention relates to a composition and a kit for detecting chlamydia pneumonia, mycoplasma pneumonia and respiratory adenovirus, the composition comprises a primer pair and a probe corresponding to the primer pair, the primer pair comprises a primer pair 1 aiming at the chlamydia pneumonia, a primer pair 2 aiming at the mycoplasma pneumonia and a primer pair 3 aiming at the respiratory adenovirus; the probes comprise a specific probe 1 aiming at chlamydia pneumonia, a specific probe 2 aiming at mycoplasma pneumonia and a specific probe 3 aiming at respiratory adenovirus. According to the invention, the optimal primer pair and probe combination is screened out from the designed six groups of specific primer pairs and probes, so that the requirement of high sensitivity is met, the detection result is highly accurate, the detection sensitivity can reach 1copies / reaction, and the anti-interference capability is relatively strong. The PCR amplification reagent in the kit is presented in the form of a freeze-dried product, can be stored at room temperature, and has stable performance within 12 months, and the detection sensitivity and precision meet the detection requirements.