Method for breeding Pseudostellaria heterophylla by virus-free tissue culture

A technology of Taizi ginseng and tissue culture, applied in the biological field, can solve the problems of high inoculation pollution, low transplant survival rate, undisclosed production process of detoxification Taizi ginseng, etc., and achieves quality enhancement, low inoculation pollution rate and fast speed. Effect

Inactive Publication Date: 2012-07-04
贵州昌昊中药发展有限公司 +1
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Problems solved by technology

The above reports did not disclose the production process of the virus-free heterophylla, high pollution of inoculation, low survival rate of transplanting, and did not propose a way to solve the virus re-infection, etc.

Method used

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Embodiment 1

[0027] A method for detoxifying tissue culture of Radix Pseudostellariae, comprising the steps of:

[0028] (1) Selection of individual plants: From April to May 2006, field observation and selection were carried out at the Pseudostellaria heterophylla production base in Dachang Town, Shibingniu, Guizhou Changhao Traditional Chinese Medicine Development Co., Ltd. The selected plants were 10-20cm high; the stems were upright, nearly square, Branches 5-8, slightly purple; leaves opposite, dark green in color, broadly ovate or ovate-rhombic, apex acuminate, base attenuated, upper glabrous, lower pubescent sparsely along the veins, top with 2 pairs of large leaves, forming a cross shape, no disease manifestations; flowers axillary or in cymes; capsules are broadly ovate, with 6-19 seeds, good single plant, well bagged and marked.

[0029] (2) Selection of explants:

[0030] Tuber bud head: the above-mentioned bagged and marked individual plants were harvested in August 2006. When...

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Abstract

The invention relates to a method for breeding Pseudostellaria heterophylla by virus-free tissue culture. The method comprises the following steps of: selecting explants, performing induction culture, detecting viruses, performing enrichment culture, performing rooting culture, propagating virus-free tissue-culture seedlings, acclimating, transplanting, and producing virus-free Pseudostellaria heterophylla stock seeds, first generation seeds and second-generation propagation seeds (production seeds). The method is easy to operate and has a low inoculation contamination rate and a high survival rate. The virus elimination operation is simple, rapid and complete, the propagation coefficient is increased by 10-15 times, and the propagation speed is high. The virus-free tissue-culture seedlings are suitable for industrialized production. The root tubers produced from the virus-free tissue-culture seedlings have resistance, are better in yield and quality than virus-infected seedlings, and can be produced commercially.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a virus-free tissue culture breeding method of heterophylla. [0002] Background technique [0003] Pseudostellariae Pseudostellariae Pseudostellaria heterophylla (Miq.) Root tuber of Pax ex Pax et Hoffm. Excavate in summer when most of the stems and leaves are withered, wash them, remove fibrous roots, put them in boiling water for a while, and then dry them in the sun or directly in the sun. Prince reference edition "Pharmacopoeia of the People's Republic of China" are all included. Radix Pseudostellariae has the functions of nourishing qi and invigorating the spleen, promoting body fluid and moistening the lungs; it is clinically used for symptoms such as spleen deficiency, fatigue, loss of appetite, weakness after illness, deficiency of qi and yin, spontaneous sweating and thirst, dryness of the lung and dry cough. The consumption of Pseudostellaria Pseudostellaria is increas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 兰才武贺定翔郑建立邓忠罗琳石胜花杨晓斌王凯军
Owner 贵州昌昊中药发展有限公司
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