221results about How to "Easy to inoculate" patented technology

A cell or tissue-culturing carrier which can effectively regenerate an intended a cell or tissue, while suppressing an excessive growth of fibroblasts, and a method of culturing a cell or tissue by using the above carrier, the cell or tissue-culturing carrier wherein fibroblasts showing substantially no growing property in a gel based on the hydrogel-forming polymer, is constituted by using a hydrogel-forming polymer; an aqueous solution of which shows a thermo-reversible sol-gel transition such that it assumes a sol state at a lower temperature and assumes a gel state at a higher temperature.

The invention relates to a method for rapidly breeding sugarcane stem tip by tissue culture and virus removal, belonging to the technical field of rapid breeding of plant by virus removal. The method comprises the following steps: carrying out disinfection pretreatment on a terminal bud of sugarcane and a cut double-bud stem section, with the terminal bud of the sugarcane and a stem tip tissue ofan axillary bud as explants, and carrying out stem tip initial culture, sprout differentiation culture, crowd sprout strong stock culture, plant root culture and virus checking by adopting a filter paper bridge culture way, thus massive virus-removed tissue culture seedlings are obtained. The invention has the advantages: the stem tip with the length of 1-2mm is taken as the explant, thus the processing operation is easy; the filter paper bridge culture way is adopted during the initial culture, thus the initiating speed is high and the perennial root dwarfing virus removing effect is thorough; and the stem tip is induced and germinated and is rapidly initiated and differentiates massive crowd sprouts, the month breeding coefficient reaches 5-8, and normomorph is realized in a long-term breeding process, thus the method is completely applicable to factory production on a large scale.

Improvements to turntables that facilitate inoculation of petri dishes. In one embodiment, the present invention is a motorized turntable for a petri dish that allows the user to use both hands for inoculation of the petri dish. In another embodiment, the present invention features an improved upper plate for a petri dish turntable that allows the user to more easily remove the petri dish from the upper plate after inoculation. In another embodiment, the present invention provides an apparatus for rotating a conventional turntable for petri dishes.

The invention discloses a method for using lily to prepare parent group to cultivate grouped and cross, belonging to plant group cultivate and cross technique. The inventive method comprises that preparing cultivate medium, using non-matured blank of lily as explant to be induced, increased, expanded, cultivated, refrigerated, transplanted, and virus detected, and using harmless tube bulb seed to plant in field, flower, and cross, to obtain lots of lily harmless seeds. The invention has the advantages in simple grafting operation, low pollution, high survival rate, low variant rate, high cross purity, high seed quality, shortened induce cultivate period, reduce cost and high practicality.

The invention provides a new technological method for preparing and industrially culturing aweto strains. The method comprises the following steps of: using young silkworms of 2 years as a host of an aweto culture medium, preparing strains by separating the tissue of wild awetos, dying the young silkworms, providing biological conditions suitable for aweto growth, and culturing aweto products with the shapes of wild awetos and the similar contents of various food mass. The invention has simple and convenient operation process, high yield rate and low culture cost.

A method for identifying rice blast resistance of rice comprises the steps of scissoring three rice stems in a large bract period from a rice growing field, bringing the rice stems back to a laboratory, scissoring 2cm from the upper portion of each stem section and 5-6cm from the lower portion of each stem section to obtain one rice stem with the whole length of approximate 7-8cm, putting the three rice stems into culture dishes with two layers of filter paper, adding sterile water of 1mL into each culture dish, washing cultivated rice blast fungus spores with the sterile water, obtaining concentration of 106 pcs / ml, dropping spore suspending liquid of 8 muL to stem section sites of each rice stem through a liquid moving gun, putting the culture dishes into a illuminating incubator at the temperature of 26 DEG C, wrapping the culture dishes with transparent films to keep humidity in the culture dishes, cultivating for ten days in light and dark alternating modes to survey disease conditions, dividing survey disease levels into three levels, regarding the first level as disease-resistant cultivars, regarding the second level as susceptible cultivars, and regarding the third level as highly susceptible cultivars. The method optimizes the concentration and inoculation amount of inoculated rice blast fungus spores and establishes simple, convenient and accurate grading standards.

The invention discloses a fermentation type lobster seasoning and a making method thereof. The method comprises the steps of: carrying out pretreatment and enzymolysis with lobster leftovers as raw materials, then carrying out fermentation with Lactobacillus helveticus capable of producing dipeptidase and tripeptidase, concentrating and extracting to prepare a compound amino acid nutritious fermentation liquor containing the active functional component of taurine; and mixing the nutritious fermentation liquor with a mixed liquid prepared from various spice herbaceous plants through the steps of frying, decocting, stewing and boiling, adding a food additive for blending, homogenizing, sterilizing to prepare the liquid or sauce type lobster seasoning. By adopting a biological technology to make the lobster seasoning, the invention overcomes the technological bottleneck on limiting the development of the lobster seasoning industry and provides a normalized and standardized making method of the special lobster seasoning, thereby promoting the rapid development of the lobster industry.

The present invention relates to a sewage treatment process by adopting improved alternative type active sludge method. Said sewage treatment process utilizes the equipments of treatment tank, anaerobic chamber, anoxybiontic chamber, aeration tank and flocculation precipitation tank, etc. and alternatively adopts the process steps of stirring, aeration, concentration, sludge reflus, mixing, flocculation, precipitation and biological dephosphorization, etc. so as to attain the goal of purifying sewage.

The invention provides an industrialized cultivation method of Morchella importuna. The method is characterized by comprising steps as follows: (1) preparation of a culture substrate; (2) preparation of a liquid strain; (3) mycelium culture; (4) fruiting management: mycelia in the step (3) are transferred to a fruiting room for fruiting management, and the fruiting management comprises management in a primordium formation promoting period, management in a mushroom bud formation period, management in a growth period and management in the manure period. According to the method, crop waste is taken as a raw material for preparation of a culture medium, liquid strains are used for sowing, industrialized cultivation of Morchella is completely realized indoor, the method is simple to operate, low in cost, short in cycle, controllable in quality and capable of realizing industrialized production all year round, and one pollution-free, pleasantly scented and delicious green product is obtained.

The invention discloses a method for preparing edible mushroom liquefied strains. The method comprises three steps, i.e. the purification and culture of primary strains, the preparation of sterilized water and the preparation and liquefaction of secondary strains. According to the method, the primary strains are cultured in a PDA (Potato Dextrose Agar)-enriched culture medium so as to obtain purified primary strains; the purified primary strains are segmented and are then inoculated into a water-soluble culture medium so as to obtain the secondary strains; and hyphae of the secondary strains are broken into pieces by using a high-speed strain crusher, and then, the secondary strains are added to the sterilized water and diluted, thereby obtaining the liquefied strains. The method has the advantages of simple process, simplicity and convenience in inoculation, quickness in strain growth, earliness in mushroom growing, good quality, high yield, high efficiency, low pollution risk, low investment and operating cost and good economic benefit. After the edible mushroom liquefied strains prepared by using the method are inoculated, the permeation of the strains is strong, and the strain growth is quick. Furthermore, the culturing and mushroom growing time of the edible mushroom liquefied strains is greatly shortened, the mushroom growing is orderly, the growing speed is high, and the yield is obviously increased.

The present invention relates to a method for virus-free tissue culture and rapid propagation of sugarcane belonging to the technical field of plant propagation. The method comprises the following steps that the terminal buds or auxiliary buds of the sugar cane is taken as the explants and the virus-free tissue culture seedlings are propagated in mass after the callus tissue inducement and culture, plant differentiation culture, proliferation culture, radication culture and virus test. The present invention is characterized in that the explants adopted are large and the operation is easy; the inoculation survival rate is high; the detoxification is simple, convenient, fast and thorough; the propagation coefficient per month is 5 to 10 times and the speed is fast which is fit for breeding in factory and commercial production.

The invention provides a novel method for preparing a third-level strain of pleurotus eryngii. The method combines traditional processes for a first-level strain, a second-level strain and a branched strain and is suitable for production of the pleurotus eryngii and other wood rotting edible fungus strains. The processes include inoculation from the first-level strain and the second-level strain to the three-level branched strain and then to a cultivating bag. A method for preparing the third-level strain of the pleurotus eryngii by using a poplar branch culture medium and a bridging material composed of broad-leaved wood sawdust, wheat bran and calcium carbonate is adopted. The method provided by the invention has the characteristic that branch is adopted for inoculating the third-level strain to the cultivating bag; the strain production process has the advantages of easiness for inoculation operation, high speed and low pollution rate; and the production cost is reduced and the production efficiency is improved by using the production process.

The invention discloses a microbial agent for producing pickles, belonging to the field of production of microbial preparations. The microbial agent product contains lactobacillus plantarum CGMCC NO.9405, and consists of the following agents: lactobacillus plantarum CGMCC NO.9405, bacillus aceticus and saccharomycetes. The preparation method comprises the following steps: singly culturing the lactobacillus plantarum, bacillus aceticus and saccharomyces cerevisiae strains, centrifuging to collect the thallus after culturing by a preset period of time, and preparing microbial powder of each strain by utilizing a conventional microbial preparation production method; and mixing and blending the prepared microbial powder according to a ratio. According to the selection and ratio of the strains, the production speed, good flavor and quality of pickle products are guaranteed, so that the production safety of the pickles is high, and the product is standard and consistent, can be suitable for large-scale industrial production and manual workshop type production and has wide application market.

The invention discloses an Agaricus Bisporus liquid spawn and a preparation method thereof. The Agaricus Bisporus liquid spawn is produced by means of three-level propagation through slant and shake-flask media and a fermenter medium. The slant and shake-flask media comprise components including 100-200g of potato, 50-150g of cow dung, 10-30g of glucose, 1-2.5g of mono potassium phosphate, 0.5-2g of magnesium sulfate and 1-4g of peptone; the fermenter medium of every 50 liters of water is composed of 2-4kg of potato, 1-3kg of cow dung, 0.5-1kg of bran, 0.2-0.4 kg of corn powder, 0.5-1kg of sucrose, 30-60g of mono potassium phosphate, 20-30g of magnesium sulfate, 120-150 mg of vitamin B and 40-50g of defoamer. By application of the Agaricus Bisporus liquid spawn and the preparation method thereof, the problem that mycelia are prone to aging and degradation during growth is solved; the liquid spawn is of globular mycelia with uniform age, high activity and fast running; a high-running spawn center is formed after the liquid spawn is inoculated to solid media, with uniform fruiting achieved; the preparation cost is only one-tenth of that of solid spawn; the growth cycle of fermenter spawn is 7-9 days, and thus spawns on a large scale can be cultured within a short time.

The invention discloses a vegetable fermentation composite bacterial agent product for pickled vegetable production, and belongs to the microbial preparation production field. The vegetable fermentation composite bacterial agent is composed of the following bacterial agents: lactobacillus plantarum CGMCC No.9405, lactobacillus rhamnosus, bacillus aceticus, and yeast. Firstly, lactobacillus plantarum, bacillus aceticus, lactobacillus rhamnosus and saccharomyces cerevisiae strains are cultured individually, are cultured for a predetermined time and then are centrifuged to collecting thalli, and powdered microbial powders of the strains are prepared by using a conventional microbial preparation production method; the prepared stain powders are mixed and blended according to the proportion, wherein the composition ratio of the stains is also obtained through carefully experimental investigation; selection and proportioning of the strains guarantee the production rate of pickled vegetable products, excellent flavor of the pickled vegetable products and good quality of the pickled vegetable products.

The invention relates to a method of tissue culturing and fast breeding pinellia tuber of toxicity-removing, comprising the following steps: employing the immature seed and / or mature seed of pinellia tuber as explant, breeding large quantity of tissue culturing sprout of toxicity-removing, bulbil of toxicity-removing and small root tuber of toxicity-removing after evoked culturing, enrichment culturing, root growing culturing, replanting and virus detection. The invention is characterized by the simple inoculation, low inoculation pollution rate, high survival rate; simple, fast and through toxicity removing; 5-10 times breeding coefficient, fast breeding, suitable for factory breeding and commercial production.

The invention provides a breeding method for a liquid strain of morchella, and also provides an industrial cultivation method for the morchella. The breeding method for the liquid strain of the morchella comprises the following steps: firstly preparing a first culture solution from potato, sawdust, water, glucose, magnesium sulfate, soil and agar, and putting a tissue of the joint of a stipe and a cap of the morchella in the first culture solution for culture to obtain a mother strain; then, preparing a second culture solution from the potato, the sawdust, the water, the glucose, the magnesium sulfate, the soil and vitamin B1, distributing the second culture solution into shake flasks, putting the mother strain in the shake flasks, and culturing to obtain a first liquid strain. The industrial cultivation method for the morchella mainly comprises the following steps: firstly performing inoculated culture on the first liquid strain to obtain a fungi bag, and then cultivating the fungi bag in soil. The industrial cultivation method for the morchella has the advantages of being short in production cycle, simple, low in infection rate, and the like.

The invention discloses a vaccination information management system based on an internet cloud technology. The vaccination information management system comprises a data acquisition module, an APP client-side, a server, a health consultation recommendation module and a high-quality commodity free recommendation module, the data acquisition module, the APP client-side and the server are connected through the internet, and the server is further connected with the health consultation recommendation module and the high-quality commodity free recommendation module through the internet, wherein a special team is in charge of both the health consultation recommendation module and the high-quality commodity free recommendation module. The vaccination information management system based on the internet cloud technology achieves informatization, intellectualization, facilitation and precision of child vaccination work, achieves the situation that parents give inoculation to a baby accurately, timely, conveniently and satisfactorily and achieves accurate statistics of population data of different age groups in a national big data era.

The invention relates to a method for breeding Pseudostellaria heterophylla by virus-free tissue culture. The method comprises the following steps of: selecting explants, performing induction culture, detecting viruses, performing enrichment culture, performing rooting culture, propagating virus-free tissue-culture seedlings, acclimating, transplanting, and producing virus-free Pseudostellaria heterophylla stock seeds, first generation seeds and second-generation propagation seeds (production seeds). The method is easy to operate and has a low inoculation contamination rate and a high survival rate. The virus elimination operation is simple, rapid and complete, the propagation coefficient is increased by 10-15 times, and the propagation speed is high. The virus-free tissue-culture seedlings are suitable for industrialized production. The root tubers produced from the virus-free tissue-culture seedlings have resistance, are better in yield and quality than virus-infected seedlings, and can be produced commercially.

The invention concerns recombinant Modified vaccinia virus Ankara comprising in the viral genome an expression cassette comprising the cowpox ATI promoter or a derivative thereof and a coding sequence, wherein the expression of the coding sequence is regulated by said promoter. The virus may be useful as a vaccine or as part of a pharmaceutical composition.

The invention relates to the technical field of the detoxication and the tissue culture of sugarcane and provides a detoxication and tissue culture rapid propagation method of chewing cane, characterized by comprising the following steps of: (1) selecting and sterilizing plants; (2) carrying out the induced culture of axillary buds; (3) carrying out differentiation culture; (4) carrying out propagation culture; (5) carrying out rooting culture; (6) transplanting; and (7) detecting viruses (germs). The detoxication and tissue culture rapid propagation method of chewing cane is characterized in that a differentiation culture medium formula in the step (3) comprises the following components: 0.001-0.01mg / L of MS and TDZ, 0.02-0.05 mg / L of IBA, 30-40 g / L of cane sugar, 100-150 mg / L of arginine and 100-150 mg / L of inositol. The invention uses chewing cane axillary buds as explants, is easy to perform vaccination operation, and reaches the high survival rate over 90 percent. By the adopted differentiation culture medium formula, cluster buds can be rapidly differentiated from induced buds in the differentiation culture process. The invention can rapidly culture chewing cane detoxication buds and multiply a monthly propagation coefficient by 5-10 times, thereby being suitable for large-scale factory production.

The invention discloses a method for liquid fermentation cultivation of Agaricus bisporus strain, which includes: firstly, inoculating activated slant culture 0.5cm2 into a 250mL culture bottle containing 60-120mL of liquid medium, controlling temperature to be 21-27 DEG C, shaking at 90-180rpm, culturing for 5-7 days to obtain primary shaking strain, transferring primary seeds accounting for 5-10% of the inoculation amount to secondary culture liquid for cultivation, transferring cultured secondary shaking liquid strain to tertiary culture liquid for cultivation, and sequentially performing all levels of cultivation to obtain seed broth by the same methods; and secondly, inoculating the seed broth accounting for 5% of the inoculation amount into a 10L fermentation jar containing 6L of fermentation medium for cultivation, and culturing at 25 DEG C for 5-6 days. The method is characterized by short production cycle, uniform fungus age, low production cost and simplicity in inoculation, a test tube of strain can be multiplied by 200000 times by five levels of cultivation, the secondary liquid strain is evidently faster than traditional solid spawn in growth speed, and the speed is increased by 33%. In addition, the growth speeds of all levels of the liquid strain are nearly equal, the average fullness time is 27.5 days, and the method is absolutely applicable to practice of factory production.

The invention discloses a new tremella cultivation method, which comprises the steps of: sawing wood for tremella cultivation into small sections, then, stacking the sawn small wood sections, and then naturally airing the stacked wood sections; loading the wood sections into a plastic bag when arachnoid cracks appear at two ends of the wood sections, tying a bag opening firmly after the wood sections are well bagged, and opening two ends of the plastic bag; and placing the well bagged wood sections into a sterilization stove for sterilization for 12-14 hours at the temperature being of 100 DEG C in the stove, cooling and taking out the wood sections, putting the wood sections into a clean greenhouse subjected to sterilization and insect killing, carrying out inoculation cultivation, and harvesting. The new tremella cultivation method disclosed by the invention is high in yield and free from diseases and pests, the produced tremella is the same as the tremella produced by cut-log cultivation and is high in market value, low in management technological requirement and small in investment quantity.

The invention discloses a bagged liquid strain inoculation machine, which is characterized by comprising a stand, wherein the stand is provided with a conveying roller and a conveyor belt arranged on the conveying roller; a drilling mechanism is arranged on the stand above the conveyor belt and comprises a bracket and a lifting device; the lifting device is connected at the upper part of the bracket; a plurality of drilling rods are connected and arranged at the lower part of the bracket; an inoculation mechanism is arranged on the stand which is positioned above the conveyor belt and at the right side of the drilling mechanism and comprises a bracket body and a lifting device which is connected and arranged at the upper part of the bracket body; and a plurality of inoculation needle heads are connected and arranged at the lower part of the bracket body and are connected with an inoculation pipeline. By adopting a setting mode of separating the drilling mechanism from the inoculation mechanism, the invention realizes the drilling and then the inoculation; and an inoculation bacterial bin is effectively formed on a bacterial bag and is convenient to carry out the inoculation, so that the inoculation quantity is easy to control, the phenomenon of blocking a needle hole cannot generate and the inoculation efficiency is high.

The invention discloses a black fungus secondary culture medium which is composed of corn cob particles, corn grains, wood dust, bran and lime. The black fungus secondary culture medium has the advantages of reasonable formula and balanced nutrition, and can well satisfy the nutritional requirements for black fungus. The corn cob is added, so that the cost of the culture medium disclosed by the invention is lower than that of other wood dust / corn grain culture media; and the bran content is increased, so that the black fungus hyphae can grow more vigorously. The invention also discloses a method for ensuring original strain survival percent of black fungus, which optimizes inoculation and culture techniques. After the inoculation, the strain survival percent is up to 100%, the inoculation survival percent of the original strain is ensured, the hypha growth rate is high, and the hyphae are white, thick, strong and vigorous and have strong growth potential.

The invention provides a liquid strain breeding method of the albumen fungus, and also provides a field bionic cultivation method of the albumen fungus. The liquid strain breeding method of Gallus firsum is: earlier use potato, sawdust, corncob powder, water, glucose, magnesium sulfate, potassium dihydrogen phosphate, peptone, vitamin B1, yeast sheet and agar to make the first nutrient solution, the The tissue at the junction of the stipe and cap of Gallus ciliariae is put into the first culture medium and cultivated to obtain the mother species; then use potatoes, sawdust, corncob powder, water, glucose, magnesium sulfate, potassium dihydrogen phosphate, peptone and Vitamin B1 is used to prepare the second culture solution, and the second culture solution is divided into shake flasks, the mother species is inserted into the shake flask, and the first liquid strain is obtained after cultivation. The biomimetic cultivation method of field field of gallinaceous fungus is mainly to first inoculate and cultivate the first liquid strain to obtain the bacterial bag, and then cultivate the bacterial bag into the soil. The field biomimetic cultivation method of Gallus fruticosa has the advantages of short production cycle, simple method and low infection rate.

The invention relates to a special matrix formula suitable for culturing mycorrhizal tobacco seedling in the condition of flue-cured tobacco seedling by floating system; all the constituents and the volume thereof are as follows: 60 percent of peat, 20 percent of perlite and 20 percent of vermiculite. The formula can improve the comprehensive quality of the tobacco seedling, cultures sound seedlings and has the advantages of wide material sources, easy acquisition, easy matrix preparation, simple production, simple inoculating operation, little fungicide dosage and easy promotion, etc. The culture medium, the inoculating technique and the corresponding Glomus intraradices Smith and Schenck are combined together, infection rate of mycorrhiza is up to over 40 percent and the effect of the mycorrhiza is good.

The invention discloses a making method of high-effective biological ferric carrier through aspergillus niger, which comprises the following steps: (1) selecting bacterial; (2) allocating spore suspension; (3) fermenting liquid; (4) extracting ferment liquid; (5) refining ferric carrier. The invention improves the activity of ferric carrier, which possesses superior using value.

The invention provides a method for preventing and treating plant soil-borne diseases and cultivating anti-bacteria cultivation soil. The method comprises the steps that firstly, during the stubble-free period in spring and summer, soil in a greenhouse or a field is deep tilled and is smashed, haulm or other straw and quick lime are evenly scattered to the soil, the soil is tilled again and watered, the soil is pressed tightly after being covered with a plastic film, and then a shed is covered tightly or sunshine soil drying is carried out; secondly, microbial manure or organic compost containing beneficial bacteria is evenly applied to the soil, ditching and ridging are carried out, and plants are prepared to be planted. The method solves the problems that the soil is easily infected by the disease bacteria again after high-temperature shed covering or sunshine soil drying is carried out, and when the microbial manure or the organic compost containing the beneficial bacteria is applied, due to the fact that the number of indigenous microorganisms in the soil is large, the beneficial microorganisms guided by applying the bacterial manure or the organic compost containing the beneficial bacteria are generally not prone to colonization and cannot play a role.

The invention relates to a sterilization system for a medium, a method for sterilization of a medium by using the sterilization system and a culture method for cordyceps militaris. The sterilization system for a medium comprises a steam generator and a sterilization cabinet connected through an exhaust pipeline, wherein the exhaust pipeline connected with the end of the sterilization cabinet is divided into two branch pipelines, one branch pipeline is straight, and a vacuum tank and a water circulating vacuum pump successively connected are installed on the other branch pipeline. According to the invention, voidage of the medium is increased, growth space of mycelia is enlarged, the medium has a fast cooling speed and a high drying degree, the purpose of improving biotransformation efficiency is finally achieved, production cost is reduced, which is favorable for popularization and application of corresponding technologies, and a high practical value and a high application value are obtained.