15results about How to "Easy to inoculate" patented technology

The invention discloses a preparation method of a viable bacterium type Chinese wolfberry enzyme, and belongs to the field of enzyme preparation. The preparation method comprises the following steps: inoculating lactobacillus rhamnosus and lactobacillus paracasei into Chinese wolfberry juice, and fermenting to obtain viable bacterium type fermented Chinese wolfberry juice; and adding auxiliary materials into the viable bacterium type fermented Chinese wolfberry juice, and sterilizing in a water bath to obtain the viable bacterium type Chinese wolfberry enzyme. According to the method, by optimizing the strain compounding proportion, the inoculum size, the fermentation time, the sterilization temperature of the lycium barbarum enzyme and the like, a simple and efficient method for preparing the viable bacterium type lycium barbarum enzyme is provided, and a foundation is laid for improving the potential value of lycium barbarum. After being sterilized in a water bath at the temperature of 40 DEG C, the viable bacterium type Chinese wolfberry enzyme obtained by the method can be ensured to contain high active substances such as flavones and polyphenols and have good antioxidant activity.

The utility model relates to the technical field of auxiliary equipment, in particular to an auxiliary device convenient for vaccination, which comprises two bases, moving mechanisms are arranged on the two bases, and the moving mechanisms are connected with four clamping plates; the moving mechanism comprises four grooves formed in the base, moving blocks are arranged in the four grooves in a sliding mode, connecting mechanisms are arranged between the four moving blocks and the four clamping plates, two bidirectional screw rods are rotatably installed on the base, the two threaded ends of the two bidirectional screw rods are in threaded connection with the moving blocks, and the two ends of the two bidirectional screw rods are in threaded connection with the moving blocks. And a driving mechanism is arranged between the two bidirectional screw rods. According to the device, the two-way screw drives the moving block to move on the base, the moving block drives the clamping plate to clamp the arm of a child, so that the arm of the child is clamped, the clamping force is not too large, the child does not feel painful in the extrusion process of the arm of the child, and medical staff can conveniently vaccinate the child.

The invention discloses a fungus inoculation device and method for fungus solid state fermentation. The fungus inoculation device comprises a storage tank, and a dust raising mechanism is arranged on one side of the storage tank; the dust raising mechanism comprises an inclined carrying table, the inclined carrying table is arranged at the bottom in the storage tank, a bearing ring is rotationally arranged on the inclined carrying table, a lower gear ring is arranged at the bottom of the bearing ring and meshed with a driving gear, the driving gear is arranged on a transmission shaft, the transmission shaft is rotationally arranged in the cavity, and the lower gear ring is meshed with the driving gear. An upper gear ring is arranged on the upper side of the bearing ring, and a plurality of stirring assemblies synchronously rotating along with the upper gear ring are evenly arranged on the inner wall of the storage tank. The storage tank is connected with the inoculation box through a material conveying pipe, and a uniform inoculation mechanism is further arranged on one side of the inoculation box. According to the application, the spore powder can be raised, meanwhile, the spore powder adhered to the storage tank and the inclined carrying table can be raised, and the spore powder can be inoculated to each position of a fermentation substrate; the inoculation uniformity and the survival rate are improved.

A cordyceps bonsai is composed of a base, a culture rack, cordyceps militaris and auxiliary parts. In the present invention, the auxiliary parts with good looks are hung on the culture rack, and the auxiliary parts with bad shapes and lack of beauty are placed in the auxiliary parts placement box of the base and hidden, which not only takes into account the aesthetics of the cordyceps bonsai, but also realizes the perfect combination of the nutrition and taste of fine wine, thereby forming a cordyceps bonsai with beautiful shape, abundant nutrients, novel characteristics, freshness and beauty. The cordyceps bonsai in the present invention is placed in a transparent wine jar as a whole, and then the freshness of the cordyceps bonsai is permanently locked with high-proof liquor. At the same time, the liquor can extract the nutrients of the cordyceps fruiting body, cordyceps culture medium, cordyceps mycelium and various auxiliary parts, forming a cordyceps wine with orange-yellow color and rich nutrition. The complex formed by the wine jar and the cordyceps bonsai is a pleasing artwork and decoration.

The invention discloses an artificial culture method and a culture medium of boletus luteus, and relates to the field of artificial culture of fungi. The SMN solid culture medium comprises the following components in parts by mass: 100-200 parts of pine needles, 10-20 parts of glucose, 1 / 50-4 / 50 parts of ferric chloride hexahydrate, 1 / 50-4 / 50 parts of sodium chloride, 1 / 20-4 / 20 parts of calcium chloride, 1 / 2-1 part of monopotassium phosphate, 1 / 4-3 / 4 parts of ammonium dihydrogen phosphate, 3 / 20-7 / 20 parts of magnesium sulfate heptahydrate, 1 / 1000000-5 / 1000000 parts of compound vitamin B, 10-20 parts of agar powder, 3-6 parts of malt paste and 1000-2000 parts of water. The SMN solid culture medium provided by the invention is suitable for culture and growth of boletus ocellatus, pollution is not easy to generate, hyphae with good growth vigor can be obtained, and a technical basis is provided for artificial culture of boletus ocellatus.

The utility model discloses an edible fungus strain inoculation device, which belongs to the technical field of strain inoculation and is characterized by comprising a device body, support frames are fixedly connected to two sides of the bottom of the device body, a transparent cover plate is arranged at the top of the device body, and four punching mechanisms are arranged on the front side of the transparent cover plate. Four cultivation bottles are arranged in the device body, an alcohol lamp is arranged in the device body, and two sealing gloves are fixedly connected in the device body; the punching mechanism comprises a fixing frame, an electric telescopic column, a punching column and a protective pad, and the problems that when most of existing edible mushroom strain inoculation is conducted, when a worker holds a tool and moves the tool into a culture bottle to conduct punching, due to the fact that the punching depth of each time is different, strain inoculation is possibly affected, and the working efficiency is high are solved. And the alcohol lamp is easy to turn over when a worker moves the cultivation bottle, and the like.

The present invention relates to the field of microneedle technology, and in particular to a chitosan-based Cu 2‑x Se microneedle and preparation method thereof, including synthesis of Cu 2‑x Se and Cu 2‑x Se-chitosan microneedles. Cu-chitosan disclosed in the present invention 2‑x Se microneedle is a patch loaded with biocompatible nanozymes. Nanozymes are composed of selenium and copper, which are trace elements required by the human body. They are prepared using PVP-assisted water precipitation to form Cu 2‑ x Se nanocrystals. Then, Cu 2‑x Se nanocrystals are dissolved in a chitosan matrix to prepare a nanozyme microneedle patch, which is then inserted into the residual tumor. The patch can retain its sustained effect in the surgical wound for more than 10 days, and the microneedle can greatly reduce the recurrence rate of the residual tumor model and inhibit the growth of the re-inoculated tumor. The microneedle also has excellent biocompatibility. The method of the present invention provides a simple and effective preparation for convenient and safe tumor vaccination.

The invention discloses treatment equipment and process applied to chemical high-nitrogen-content wastewater, the treatment equipment comprises a pretreatment system, a chemical treatment system is arranged on one side of the pretreatment system, and a deep treatment system is arranged on the front side of a biological treatment system. According to the method, a biological treatment technology and a chemical oxidation technology are combined, and most biodegradable organic matters are removed in the early stage by utilizing the efficient degradation capability of the biological treatment technology; for wastewater simultaneously containing high-concentration and refractory organic substances, the biodegradability of the wastewater is improved, the organic substances are eliminated, the COD (Chemical Oxygen Demand) concentration is reduced, competition with biological denitrification obligate bacteria is avoided, and the normal denitrification reaction process is inhibited through an efficient anaerobic means assisted by technologies such as chemical oxidation and the like. For wastewater with relatively high ammonia nitrogen concentration, the biochemical combination method not only can realize the ammonia nitrogen removal rate up to 98%, but also can obviously reduce the COD (Chemical Oxygen Demand) concentration in the wastewater, thereby achieving the targets of efficient deamination and pollution emission reduction.

The invention belongs to the technical field of vaccines, and provides a canine distemper subunit vaccine spray which is formed by dissolving nanoparticles carrying key antigen epitope peptide in normal saline in the form of freeze-dried powder. Comprising nanoparticles carrying key antigen epitope peptide and a buffer solution, and the nanoparticles are dissolved in the buffer solution in the form of freeze-dried powder; the mass ratio of the nanoparticles to the buffer solution in the spray is (0.1-10): 1; the nano-particles are composed of epitope peptide of the hemagglutinin protein of the canine distemper virus and a nano-carrier. The spray type canine distemper vaccine is convenient to store and transport, meanwhile, the stability of antigens, the convenience of inoculation and the safety and effectiveness of the vaccine are guaranteed, and large-scale inoculation of the vaccine can be rapidly promoted.

The invention relates to the technical field of fermentation strain preparation, and discloses clostridium butyricum freeze-dried powder and a preparation method thereof, and the freeze-dried powder comprises the following raw materials: clostridium butyricum liquid, a carrier, a freeze-drying protective agent and a dispersion stabilizer. The clostridium butyricum freeze-dried powder is arranged, the viable count of the clostridium butyricum freeze-dried powder is larger than 2 * 10 < 9 > cfu / g, the water content is smaller than 2%, and spores are in a dormant state, so that long-time preservation of the strain is facilitated, the strain cannot be degraded in the preservation process, the metabolic system cannot be affected, and the number of the strain in the inoculation process can be reduced. Due to decline caused by continuous passage and propagation and the clostridium butyricum in a spore dormant state, inoculation in fermentation production is facilitated, the inoculated dry powder strain can quickly germinate spores to form vegetative cells, inoculation steps are reduced, strain activity is maintained, and in industrial fermentation production, the clostridium butyricum has a good application prospect. The production cycle can be shortened by using the dry powder strain, the probability of spontaneous mutation in microbial passage is reduced, and the production efficiency is improved.

The utility model provides a treatment device for semi-fine wool sheep, and belongs to the field of animal medical instruments. Comprising a protective frame which comprises an inoculation area and a waiting area; the channel part is located on the protection frame and used for controlling the flock of sheep to pass through the inoculation area one by one so that the semi-fine wool sheep can complete vaccination; and the limiting part is mounted on the channel part and is set to automatically close the inlet of the channel part after the semi-fine wool sheep enters the channel part, so that the next semi-fine wool sheep cannot enter the channel part and waits for entering the channel part in the waiting area. Through the arrangement of the limiting part, after entering the channel part, the semi-fine-wool sheep continues to move forwards to extrude the extrusion plate, so that the limiting part is driven to rotate, one end of the limiting plate moves into the limiting groove, an inlet of the channel part is closed, the next sheep is stopped from entering, and meanwhile, after the semi-fine-wool sheep in the channel part is inoculated, the semi-fine-wool sheep enters the channel part. And when the robot continues to walk forwards, after the rotating shaft is extruded, the limiting block moves, and therefore limiting on the limiting plate is relieved.

The utility model discloses a culture dish for microbial cells, belongs to the technical field of culture dishes, and solves the problem that when an existing device is used for flora inoculation, a certain opening and closing angle is formed between a bottom box and a cover inconveniently with a single hand, and consequently strain lineation is inconvenient, the culture dish comprises a dish body and a dish cover, the dish cover is clamped to the upper portion of the dish body, and the dish cover is clamped to the lower portion of the dish body. A rotating assembly is arranged on the outer side of the dish body and the outer side of the dish cover and comprises a rotating ring, a lug plate, a threaded rod, a nut, a rotating rod and a connecting block, the rotating ring is rotationally arranged on the outer side of the dish body, and the positions of the dish body and the dish cover can be limited by arranging the rotating ring, the lug plate, the threaded rod, the nut, the rotating rod and the connecting block; the situation that the dish cover falls off from the dish body is avoided, the dish cover can incline with the rotating rod as the axis so that microbial cells can be conveniently inoculated into the dish body, the purpose that the dish body and the dish cover can conveniently form a movable opening and closing angle to facilitate inoculation is achieved, and use is easy and convenient.

The utility model discloses a kind of for yak breeding young cattle breeding device, it is related to young cattle breeding technical field, including drive assembly, the drive assembly is composed by drive mechanism and blocking mechanism.Just injection mechanism can reserve a large number of vaccine liquid for use, avoid the action of repeatedly pumping liquid when injecting, drive mechanism can control injection mechanism to realize the function of automatic pumping and injecting liquid, it is convenient to inoculate and inject operation to more young cattle vaccine, and blocking mechanism can adjust and control the pumping amount and injection amount of medicament when injection mechanism single injection, dose control accurate, when injecting vaccine to young cattle currently, still using traditional syringe to inject, when needing to inoculate and inject vaccine to more young cattle, it needs repeatedly pumping vaccine liquid and injection operation, very cumbersome, and pumping amount and injection amount each time need artificial visual judgment and control, the problem of poor accuracy.

The invention belongs to the technical field of nano materials, and particularly relates to a nanosheet-based tumor antigen complex tumor vaccine and a preparation method thereof, the preparation method comprises the following steps: synthesizing nanosheets and preparing the vaccine, after a part of tumor tissues are obtained, co-culturing the nanosheets and tumor cells, and enabling the tumor cells to fully take in the nanosheets. Near-infrared laser is irradiated, and through the photocatalytic effect of the nanosheets, tumor cells generate a strong immune effect, break and release a large number of tumor antigens. And due to the physical properties of multilayer stacking and roughness of the nanosheets, tumor antigens can be adsorbed on the surfaces of the nanosheets, so that the antigen uptake of immune cells is improved, and the immune escape effect is reduced. According to the method provided by the invention, the preparation difficulty of the tumor vaccine is greatly reduced, the uptake rate of the tumor antigen is improved, and a simple and effective preparation is provided for preparing the convenient and safe tumor vaccine inoculation.

The invention relates to the field of animal health. The present invention provides an attenuated infectious bronchitis virus (IBV). The attenuated infectious bronchitis virus can protect chickens from IBV infection.