5 results about "Virus elimination" patented technology

The invention discloses a method for virus-free rapid propagation by utilizing strawberry anther culture, and relates to the technical field of strawberry anther culture, and the method is characterized by comprising the following steps: S1, selection and disinfection of explants, S2, culture stage, S3, ploidy identification, S4, virus detection, and S5, domestication and transplanting of virus-free seedlings. According to the strawberry anther in-vitro culture method, a large number of virus-free plants are successfully obtained through a strawberry anther in-vitro culture technology, the strawberry detoxification efficiency and effect are remarkably improved, and technical support is provided for healthy development of the strawberry industry.

The application discloses application of dihydroquercetin in passion fruit virus elimination and application of Pedu CYP75B gene in disease-resistant breeding, and belongs to the technical field of tissue culture virus elimination. The application discloses application of dihydroquercetin in preparation of a passion fruit virus elimination preparation and application of Pedu CYP75B gene in cultivation of a disease-resistant passion fruit variety, wherein the gene is used for increasing synthesis of dihydroquercetin, and the Pedu CYP75B gene is selected from the nucleotide sequences shown in SEQ ID NO. 5-SEQ ID NO. 7. The application further discloses a virus elimination method for passion fruit, which comprises the step of inoculating micro stem tips of passion fruit into a culture medium added with dihydroquercetin and culturing. The application provides a new scheme for virus-free seedling production and disease-resistant breeding of passion fruit.

The present application relates to the technical field of medicinal plant tissue culture, in particular to a rapid virus-free cloning method and reagent combination for medicinal plant tissue culture. The method comprises the following steps: pretreatment and disinfection of explants, precise virus elimination, preparation of dynamic adaptive medium, rapid propagation, real-time high-throughput detection of virus-free, seedling raising and transplanting, etc. The reagent combination comprises a multifunctional degradable reagent kit, intelligent response type nano disinfection microcapsules, special reagents such as composite virus elimination liquid, etc. The virus elimination rate is greater than or equal to 95%, the survival rate of explants is greater than or equal to 90%, the propagation cycle is shortened by 30%, the detection time is less than or equal to 2 hours, the survival rate of transplanting is greater than or equal to 85%, and the degradation rate of reagents is greater than or equal to 90%. The present application is suitable for herbaceous, woody and liana medicinal plants of the families of Araliaceae, Eucommiaceae, Orchidaceae and Campanulaceae, etc., can realize stable virus elimination and propagation effect, significantly improve the virus-free cloning efficiency and seedling quality, and meet the green production demand.

The invention belongs to the technical field of strawberry stem tip detoxification, and particularly relates to a strawberry stem tip ultralow temperature detoxification method and a culture medium thereof. The invention relates to a strawberry stem tip ultralow temperature detoxification method. The method comprises the steps of explant selection, pretreatment, gradient vitrification dehydration, ultralow temperature freezing, segmented thawing, recovery treatment, dynamic recovery culture and seedling hardening. The strawberry stem tip ultralow-temperature detoxification method and the culture medium provided by the invention overcome the defects of low stem tip survival rate, unstable detoxification effect, complicated operation, high production cost, poor regenerated plant quality and the like in the existing strawberry ultralow-temperature detoxification technology; the ultralow-temperature strawberry detoxification technology which is high in detoxification rate, high in stem tip survival rate, simple and convenient to operate, adaptive to different strawberry varieties and capable of being applied in a large scale is provided, the technical bottleneck existing in strawberry detoxification seedling culture in China is solved, the quality improvement and upgrading of the strawberry industry are promoted, and a technical support is provided for large-scale culture of strawberry virus-free seedlings.

The invention discloses a culture medium for tissue culture and rapid propagation of Bo-chrysanthemum and a tissue culture and rapid propagation method, and aims to solve the problems of low efficiency, easiness in carrying viruses and germplasm degeneration of a traditional propagation mode. The method comprises the following steps: taking stem tips of underground foot buds of Bo-chrysanthemum as explants, sterilizing, inoculating into an MS culture medium containing specific hormones, and sequentially performing primary induction, subculture multiplication and rooting culture to obtain complete test-tube plantlets. And finally transplanting the seedlings to a field through gradual seedling hardening and special matrix transition. According to the method, standardization and high efficiency of the breeding process are achieved, the breeding coefficient is high, the production period is short, seedling detoxification is thorough, genetic characters are consistent, the transplanting survival rate is high, transplanting and harvesting can be conducted in the current year, the quality, yield and production benefits of bo-chrysanthemum seedlings are remarkably improved, and the method is suitable for large-scale application and popularization.