14 results about "Virus elimination" patented technology

A method for ultra-fast inactivation of viruses based on periodate, which relates to the technical field of waterborne virus disinfection. This method forms a mixed system by adding a periodate oxidant and a homogeneous trivalent ruthenium ion activator of transition metals into the water body containing the virus to be treated, and then rapidly and thoroughly inactivates the virus. The present invention uses a homogeneous transition metal ruthenium ion catalyst to activate periodate, which not only destroys the culturing ability of the virus, but also destroys the nucleic acids encoding protein and RNA replication in the virus, and at the same time affects the functions of attachment and injection into the host during virus proliferation, achieving the complete inactivation of the virus. This method has a wide range of applications, does not require additional energy and instrumentation, saves energy, and is simple to operate.

The present disclosure is directed to a novel redesigned or retrofitted cabin air filtration system utilizing an ultraviolet light, radiation, or an energy matrix membrane and related technology to eliminate toxins such as viruses, bacteria, mold, fungi, rickettsia, protozoa, etc. from the air system in a vehicle, such as an automobile, marine vehicle airplane, etc., thereby improving the current cabin air filter functionality and providing a sterile air distribution environment throughout the cabin of the vehicle or small area.

The invention discloses a method for regulating and controlling detoxification of garlic aerial bulbs by using multi-walled carbon nanotubes, relates to the technical field of plant detoxification, and solves the problems of low detoxification efficiency of garlic aerial bulbs, great influence of detoxification on growth and development of the garlic aerial bulbs and the like in an existing method. According to the technical scheme, the method is characterized by comprising the following steps: (a) treating a multi-walled carbon nanotube to enhance the adsorption capacity of the surface of the multi-walled carbon nanotube so as to enhance the adsorption capacity of the multi-walled carbon nanotube to viruses; (b) dispersing the treated multi-walled carbon nanotubes in water to prepare a suspension, which has the effects that the method for regulating the detoxification of the garlic aerial bulbs by using the multi-walled carbon nanotubes not only has an efficient detoxification effect, but also has small influence on plant growth, is simple and convenient to operate, has significant advantages in purification methods, can effectively improve the plant quality and economic benefits, and is suitable for industrial production. Meanwhile, environmental pollution is reduced.

The invention discloses a method for virus-free rapid propagation by utilizing strawberry anther culture, and relates to the technical field of strawberry anther culture, and the method is characterized by comprising the following steps: S1, selection and disinfection of explants, S2, culture stage, S3, ploidy identification, S4, virus detection, and S5, domestication and transplanting of virus-free seedlings. According to the strawberry anther in-vitro culture method, a large number of virus-free plants are successfully obtained through a strawberry anther in-vitro culture technology, the strawberry detoxification efficiency and effect are remarkably improved, and technical support is provided for healthy development of the strawberry industry.

The disclosure provides methods and compositions utilizing recombinant nucleic acid constructs or a replication incompetent virus-like particle encoding a chemokine, cytokine, or apoptosis inducing protein (e.g. Caspase 9 (Casp9)), or other toxins in a form which can only be transcribed in the presence of a viral polymerase. These methods can be adapted to target many viral infections and reduce or eliminate viral load, and provide a fundamentally different treatment for viral infections.

The application discloses application of dihydroquercetin in passion fruit virus elimination and application of Pedu CYP75B gene in disease-resistant breeding, and belongs to the technical field of tissue culture virus elimination. The application discloses application of dihydroquercetin in preparation of a passion fruit virus elimination preparation and application of Pedu CYP75B gene in cultivation of a disease-resistant passion fruit variety, wherein the gene is used for increasing synthesis of dihydroquercetin, and the Pedu CYP75B gene is selected from the nucleotide sequences shown in SEQ ID NO. 5-SEQ ID NO. 7. The application further discloses a virus elimination method for passion fruit, which comprises the step of inoculating micro stem tips of passion fruit into a culture medium added with dihydroquercetin and culturing. The application provides a new scheme for virus-free seedling production and disease-resistant breeding of passion fruit.

The present application relates to the technical field of medicinal plant tissue culture, in particular to a rapid virus-free cloning method and reagent combination for medicinal plant tissue culture. The method comprises the following steps: pretreatment and disinfection of explants, precise virus elimination, preparation of dynamic adaptive medium, rapid propagation, real-time high-throughput detection of virus-free, seedling raising and transplanting, etc. The reagent combination comprises a multifunctional degradable reagent kit, intelligent response type nano disinfection microcapsules, special reagents such as composite virus elimination liquid, etc. The virus elimination rate is greater than or equal to 95%, the survival rate of explants is greater than or equal to 90%, the propagation cycle is shortened by 30%, the detection time is less than or equal to 2 hours, the survival rate of transplanting is greater than or equal to 85%, and the degradation rate of reagents is greater than or equal to 90%. The present application is suitable for herbaceous, woody and liana medicinal plants of the families of Araliaceae, Eucommiaceae, Orchidaceae and Campanulaceae, etc., can realize stable virus elimination and propagation effect, significantly improve the virus-free cloning efficiency and seedling quality, and meet the green production demand.

The present invention relates to the technical field of virus-free rapid propagation of fruit trees, and particularly to a virus-free method for passion fruit. The method includes: inoculating the axillary bud-bearing stem segments of passion fruit into a vitrification induction medium for inducing plant vitrification to obtain vitrified plants; inoculating the obtained vitrified plants into a recovery induction medium for recovery culture to achieve virus elimination of passion fruit. Using the obtained normal adventitious buds as the base number, cutting the leaves for detection of major virus diseases, and screening to obtain virus-free passion fruit seedlings, and the virus elimination efficiency can reach 95%.

The invention belongs to the technical field of strawberry stem tip detoxification, and particularly relates to a strawberry stem tip ultralow temperature detoxification method and a culture medium thereof. The invention relates to a strawberry stem tip ultralow temperature detoxification method. The method comprises the steps of explant selection, pretreatment, gradient vitrification dehydration, ultralow temperature freezing, segmented thawing, recovery treatment, dynamic recovery culture and seedling hardening. The strawberry stem tip ultralow-temperature detoxification method and the culture medium provided by the invention overcome the defects of low stem tip survival rate, unstable detoxification effect, complicated operation, high production cost, poor regenerated plant quality and the like in the existing strawberry ultralow-temperature detoxification technology; the ultralow-temperature strawberry detoxification technology which is high in detoxification rate, high in stem tip survival rate, simple and convenient to operate, adaptive to different strawberry varieties and capable of being applied in a large scale is provided, the technical bottleneck existing in strawberry detoxification seedling culture in China is solved, the quality improvement and upgrading of the strawberry industry are promoted, and a technical support is provided for large-scale culture of strawberry virus-free seedlings.

Methods and compositions are provided for reducing virus titer and eliminating virus-infected cells from an individual.

The invention discloses a culture medium for tissue culture and rapid propagation of Bo-chrysanthemum and a tissue culture and rapid propagation method, and aims to solve the problems of low efficiency, easiness in carrying viruses and germplasm degeneration of a traditional propagation mode. The method comprises the following steps: taking stem tips of underground foot buds of Bo-chrysanthemum as explants, sterilizing, inoculating into an MS culture medium containing specific hormones, and sequentially performing primary induction, subculture multiplication and rooting culture to obtain complete test-tube plantlets. And finally transplanting the seedlings to a field through gradual seedling hardening and special matrix transition. According to the method, standardization and high efficiency of the breeding process are achieved, the breeding coefficient is high, the production period is short, seedling detoxification is thorough, genetic characters are consistent, the transplanting survival rate is high, transplanting and harvesting can be conducted in the current year, the quality, yield and production benefits of bo-chrysanthemum seedlings are remarkably improved, and the method is suitable for large-scale application and popularization.

To provide devices for viral, microbial and pathogen control and removal.SOLUTION: A device comprises a carrier material comprising a carbohydrate-based polymer, and a binding agent. The binding agent is attached by one or more covalent bonds to the carrier material, and the binding agent can bind to a target, the target being one or more of a biotoxin, a virus, a microbe, and a microbial component.SELECTED DRAWING: Figure 3A

The invention discloses a method for obtaining homozygous anti-polyhedrosis individuals through egg-stage virus inoculation screening, belongs to the technical field of silkworm genetic breeding, and solves the problems that traditional larva virus addition screening is delayed in time efficiency and virus leaf preparation procedures are tedious. According to the technical scheme, the method comprises the following steps: cutting egg rings and marking information in a silkworm embryo withering period; immersing the egg surface of the egg ring downwards into a BmNPV suspension with the concentration of 5 * 10 <-1 > * 10 <-1 > PIBs / ml for 30 seconds; taking out, airing, putting into a silkworm tray, and covering with disinfected black shading cloth until dawn 5 on the second day after dint of green; removing the black-out cloth to enable egg surfaces to sense light, collecting the ants within 1-2 hours, and presetting feed; and feeding to a one-year-old sleep stage to a two-year-old silkworm raising stage, and screening survival individuals as disease-resistant candidates. According to the method, larva poison adding is replaced with poison inoculation in the egg period, viruses are delivered through the egg shell eating behavior when newly-hatched silkworms are hatched, and the method is mainly used for efficiently obtaining silkworm homozygous individuals resistant to the polyhedrosis, remarkably shortening the breeding period and eliminating the virus leaf preparation link.

The present invention is a disinfectant distribution system for conveniently eliminating viruses and diseases. In particular, the invention allows a user to conveniently install the system in public and private spaces to help improve life. The proposed embodiment provides a unique experience for a user by providing a safe experience for the general public.