The present application relates to the technical field of medicinal
plant tissue culture, in particular to a rapid
virus-free
cloning method and
reagent combination for medicinal
plant tissue culture. The method comprises the following steps: pretreatment and disinfection of explants, precise
virus elimination, preparation of dynamic adaptive medium, rapid propagation, real-time high-
throughput detection of
virus-free,
seedling raising and
transplanting, etc. The
reagent combination comprises a multifunctional degradable
reagent kit, intelligent
response type nano disinfection microcapsules, special reagents such as composite
virus elimination liquid, etc. The
virus elimination rate is greater than or equal to 95%, the
survival rate of explants is greater than or equal to 90%, the propagation cycle is shortened by 30%, the detection time is less than or equal to 2 hours, the
survival rate of
transplanting is greater than or equal to 85%, and the degradation rate of reagents is greater than or equal to 90%. The present application is suitable for herbaceous, woody and
liana medicinal plants of the families of
Araliaceae, Eucommiaceae,
Orchidaceae and
Campanulaceae, etc., can realize stable
virus elimination and
propagation effect, significantly improve the virus-free
cloning efficiency and
seedling quality, and meet the
green production demand.