49 results about "Β elimination" patented technology

A positive resist composition is provided comprising (A) a resin component having a carboxylic acid moiety protected with an acetal protective group which is decomposable under the action of an acid, wherein in the carboxylic acid moiety protected with an acetal protective group, deprotection occurs not by way of β-elimination, and (B) a photoacid generator. The resist composition exhibits a high resolution when processed by ArF lithography.

The invention belongs to the technical field of medicine, and relates to pegylated celastrol and a preparation method and application thereof. The pegylated celastrol has a structure shown as a general formula in the specifications. The invention also provides application of the pegylated celastrol and methoxy amino polyethylene glycol 10kDa mono-modified celastrol (mPEG10k-NHCO-celastrol) to preparation of medicines for treating various malignant tumors such as prostatic cancer, lung cancer, liver cancer and cervical cancer. Celastrol is subjected to chemical structural modification by PEG, so that the problem that the celastrol has low solubility can be solved; and an injection can be prepared after the celastrol is modified by the PEG, so that the elimination half life of the celastrol can be prolonged through in-vivo injection, the toxic and side effects of the celastrol are reduced, and the administration frequency of a patient is reduced.

The invention provides a yak blood antioxidative peptide with intnstive antioxidation activity. The antioxidative peptide has a sequence of TLPDTEKQIKKQ, has reducing power up to 3.24 mg*mL<-1>, has a lipid peroxidation inhibition capability up to 2.43 mg*mL<-1>, an OH elimination capability up to 0.74 mg*mL<-1>, an ABTS<+> elimination capability up to 1.58 mg*mL<-1>, a DPPH elimination capability up to 1.59 mg*mL<-1>, a total antioxidation capability up to 4.27 mg*mL<-1>, has very good use prospects, and can be used for preparing corresponding antioxidants and applied to related antioxidation application fields.

In estimating a structural formula of an unknown substance produced through partial structural change of an original substance having a known structure caused by metabolism or the like, structural change is considered in two stages, the elimination of a partial structure and the addition of another partial structure. First, an additional partial structure is collected as known information in addition to an MSⁿ spectrum of the unknown substance and a structural formula of the original substance. A structural formula at the time when a partial structure is eliminated from the original substance is estimated, and a structural formula of each of product ions is estimated. The structural formula of the unknown substance is determined by estimating a structure that can produce the candidates for structural formulas of the product ions by dissociation.

An intermediate for preparing (meth)acrylated photoinitiators according to Formula (I):

wherein:

• • R1 is selected from the group consisting of hydrogen and a methyl group;
  • A represents a group including at least one photoinitiating moiety;
  • L represents a n+o-valent linking group including at least one carbon atom;
  • n and o each independently represent an integer from 1 to 4;
  • p is equal to 0 or 1;
  • X represents a group selected from the group consisting of Cl, Br, I, and R²SO₃; and
  • R² represents an optionally substituted group selected from the group consisting of an alkyl group, an alkenyl group, an alkynyl group, an alkaryl group-, an aralkyl group, an aryl group and a heteroaryl group. Also, a method for the preparation of (meth)acrylated photoinitiators by β-elimination of HX from the intermediate according to Formula (I).

The invention belongs to the technical field of medicine and medical engineering, and particularly relates to injection for treating AI (avain in-fluenza). The injection comprises, in percentage by mass, 5%-10% of a BS (bursin active peptide solution), 30%-45% of traditional Chinese medicine extracts with radix pseudostellariae as a main material and 50%-60% of water. BS, the traditional Chinese medicine extracts with the radix pseudostellariae as the main material and the water are proportioned in percentage by mass and mixed and filtered for sterilization with double membranes, namely a filter membrane with the specification of 0.45 mu m and a filter membrane with the specification of 0.25 mu m. The efficacy of the injection is remarkably improved, the injection has efficient symptom and root cause treatment effects and has the effects of healthy energy supporting, evil elimination, plague clearing and detoxification, AI is not prone to relapse after being cured, drug resistance is unlikely to be caused, the AI cure rate is high, and the injection can be stored at normal temperature, is environment-friendly, efficient, high-quality and free of residues and toxic and side effects and is a novel specific medicine for treating AI.

The invention belongs to the technical field of detection. The invention discloses a method for evaluating the anti-aging efficacy of cosmetics. According to the method, indexes such as a beta-galactosidase activity inhibition effect, a telomerase activity enhancement effect, an oxygen free radical elimination effect, a lipid browning content, a survival rate and the like are evaluated. Accordingto the invention, experimental operation and observation are facilitated, and since chemical substances are directly absorbed into a circulating system, and painful and time-consuming injection or invasive surgery is not needed, so that pressure or tissue damage related to a rodent aging model is avoided. Zebra fish is equivalent to mice for 8-10 days in one day, the experiment period is shorter,and the cost is low; The zebra fish, as a complete living body model, can be used for high-throughput screening of drugs, especially screening of chemical drugs. Embryos and juvenile fishes are transparent, various tissue and organ changes can be directly observed under a microscope, and experiment results are visual and easy to understand.

Conjugates of SN-38 that provide optimal drug release rates and minimize the formation of the corresponding glucuronate are described. The conjugates release SN-38 from a polyethylene glycol through a β-elimination mechanism.

The present invention studies expression levels of a nuclear receptor PXR and a cytochrome enzyme CYP3A in various tissues of ietalurus punetaus and screens target tissues of gills and foreguts respectively for detecting activities of the PXR and CYP3A, a monomer extracted and purified from natural products is used to conduct mouth-filling on the ietalurus punetaus, the gills and foreguts are collected, the expression levels of the nuclear receptor PXR and cytochrome P450 3A enzyme gene are detected, the natural product monomer of rotenone capable of activating a nuclear receptor PXR pathway and regulating the CYP3A enzyme is screened out, an experiment proves that the rotenone can shorten the residual time of enrofloxacin in muscle with skin (edible tissues) of the ietalurus punetaus from12 d to 7 d, and also significantly reduces the residual amount at the same time point, thus the rotenone can be used to accelerate elimination of quinolone chemical residues in the ietalurus punetaus body, and is of great significance for ensuring quality and safety of aquatic products and reducing risks of intake of the aquatic products containing chemical drug residues by the human body.

The invention discloses an anti-alcoholic dew and a preparation method thereof. The anti-alcoholic dew is characterized by being prepared from the following raw materials in parts by weight: 30-40 parts of corn peptide, 10-20 parts of vinegar, 20-30 parts of honey, 10-20 parts of fructus lycii, 20-30 parts of mulberries, 10-30 parts of rhizoma polygonati, 30-40 parts of radix astragali, 5-10 partsof radix puerariae and a proper amount of water. Compared with the prior art, the anti-alcoholic dew has homology of medicine and food, takes the corn peptide as a main component, can inhibit the absorption of alcohol by the stomach, increases the activity of ethanol dehydrogenase and acetaldehyde dehydrogenase in vivo, and promotes the metabolism and discharge of alcohol in vivo; alcohol compounds can be quickly decomposed by drinking the anti-alcoholic dew before or after meals, so that the symptoms of patients with severe alcoholic liver can be gradually improved, and the anti-alcoholic dew has good functions of dispelling the effects of alcohol and protecting the liver. Besides, all the components play a synergistic role, so that a good hangover alleviating and liver protecting effectis achieved, and the anti-alcoholic dew is non-toxic and harmless, and can improve the ADH activity of liver tissues, promote ethanol metabolism, accelerate the elimination rate of ethanol metabolites, reduce damage to tissues and cells, play a role in alleviating hangover and promoting awakening, and protect the liver.

The invention provides an andansetron composition for injection, relating to the technical field of drugs and drug preparation. The main drugs of the andansetron composition include andansetron and melatonin, wherein the melatonin comprises a quick-release part and a cyclodextrin encapsulated slow-release part. The andansetron composition for injection, provided by the invention, has the advantages that the treatment effect of the andansetron is improved, the instability of the MT (melatonin), caused by the oral absorption of the MT and the fast distribution and elimination of the MT are avoided, the first-pass effect of the MT is reduced, the dosage of the andansetron is reduced, the quick-release and slow-release combined drug administration design is in line with the physiological secretion characteristic of the MT, the problem of short half-life period of the MT is solved, the biological availability of the product is improved, the melatonin has a synergistic effect on the andansetron for CINV (Chemotherapy Induced Nausea And Vomiting) treatment, after the melatonin and the andansetron are combined, the CINV can be treated, the treatment effect of the andansetron on the CINV are improved, the treatment course is shortened, the usage and the side effect of the andansetron are reduced, and the immunity of human bodies can be improved; and a certain concentration of melatonin maintained in the blood of the human body can be used for effectively reducing the stress reaction of organisms and is facilitated to the treatment of the CINV.

The invention relates to the technical field of collagen production, in particular to a preparation method of fishskin collagen with certain-range molecular weight. The preparation method specificallycomprises the following steps of: preprocessing fishskin, carrying out enzymolysis membrane cycling separation, carrying out decoloration and fishy smell elimination processing, and carrying out freeze drying. Through specific combined technical steps and parameter conditions, fishskin preprocessing, enzymolysis membrane cycling separation, decoloration and fishy smell elimination processing andfreeze drying are carried out to obtain collagen powder of which the molecular weight is 2-5KD, the triple helix structure of the prepared fishskin collagen is not damaged, and original biological activity is still kept; main ingredients are the 2-5KD collagen powder which has good water solubility and can be diluted by water at any ratio; the fishskin collagen is from the collagen of vertebrates,has good homology and compatibility with the muscle body and the skin of people and has similar molecular weight and structure with the people; and after the fishskin collagen is smeared to the skin,the fishskin collagen can be quickly dissolved into the epidermis, and collagen polypeptide of which the molecular weight is smaller than 5kDa has various physiological activities, including blood pressure reduction and oxidization resistance.

The invention relates to design, synthesis and application of a near-infrared fluorescence imaging agent for a targeted tumor VEGFR-3 molecules, of which the structural formula is ICG-OSu-(PEG)n-G(CGLARGRGC), wherein ICG is near-infrared fluorescence imaging agent indocyanine green, ICG-OSu is sulfonic acid group indocyanine green activated ester, the core LARGR of cyclic polypeptide G(CGLARGRGC)is polypeptide TMVP1 of a targeted VEGFR-3 molecule, which has carboxyl reactivity, and both are bridged through polyethylene glycol (PEG), wherein n is an integer from 2-20. According to the near-infrared molecule imaging agent provided by the invention, the specificity of ICG development cervical cancer, breast cancer focus and lymphatic metastasis focus thereof is greatly improved, and good instructions are provided for later clinical diagnosis of cervical cancer and breast cancer as well as elimination of tumor metastasis lymph node by applying a fluorescent endoscope.

The invention relates to a molecular marker related to the rectal cancer and application thereof, and belongs to the technical field of the medical molecular biology. According to the invention, two key designs are carried out; one design is that ctDNA detection is carried out by applying a whole-exon big panel of 425 cancer-related genes instead of a small panel of a small number of hotspot genes, so that not only is relevance between one single gene mutation and an nCRT treatment effect obtained, but also relevance between genetic mutations of a plurality of signal channels and the nCRT treatment effect is obtained; and the other design is that a plurality of monitoring time points (four time points) are set before an operation, the big panel of the 425 genes is combined, and meanwhile,elimination of the mutations in the ctDNA and dynamic changes of acquired mutations are monitored. Value of ctDNA dynamic monitoring in predication of the nCRT treatment effect is shown, and a new opinion is proposed for a patient adopting a W&W strategy. The invention proves effects of ctDNA detection in the early period of predicting the prognosis of an LARC patient.

The invention discloses a method for preparing a monoclonal antibody through rapid immunization, which belongs to the technical field of diagnosis, and comprises the following steps: S1, taking animals which are not less than 7 weeks old from cultured animals for cage separation, S2, anesthetizing the animals by using 4% chloral hydrate according to the amount of 0.1 ml/10g, S3, extracting a diluted antigen solution by using a 1ml disposable sterile syringe, and S4, fixing the narcotized animal on an operating table in a clean environment, exposing the abdomen, and removing hairs from the abdomen; S5, cutting off the outer epidermis of the abdomen by using a pair of surgical curved scissors, pushing aside adipose tissues, slightly pulling out the spleen by using tweezers, and injecting anantigen into the spleen; S6, suturing the wound by using a suture needle; S7, carrying out second and third immunization every 3-14 days. Blood is collected 25 days after the third immunization to verify the antibody secretion condition of the sensitized B lymphocytes; the invention has the advantages of reasonable and practical design, low antigen usage amount, reduced cost, improved antibody titer, and elimination of false positive to some extent. The immune operation is simple, and the situation of unsuccessful immunization caused by immunization to the subcutaneous part does not need to beworried.

The invention provides an application of a nanoscale coordination polymer Hemin (at) Gd-NCPs, the Hemin (at) Gd-NCPs is used for enhancing radiotherapy, and oxidative stress induced by radiotherapy can be amplified through two aspects of X-ray deposition and GSH elimination. Radiotherapy-induced tumor cell immunogenicity death is enhanced by amplifying radiotherapy-induced oxidative stress, release of DAMPs is promoted, and DC cell activation is effectively promoted; the anti-tumor immune response of an organism is effectively activated by utilizing the radiotherapy sensitization effect mediated by the Hemin-coated Gd-NCPs, the intratumor infiltration of CD4 < + >/CD8 < + > T cells is promoted, the intratumor IFN-gamma content is increased, the tumor immunosuppression microenvironment is improved, and the synergistic effect of radiotherapy and an immune checkpoint inhibitor is enhanced when the Hemin-coated Gd-NCPs is combined with an inhibitor alphaPD-L1; the combination of radiotherapy and alphaCTLA-4 can effectively inhibit liver and lung metastasis of 4T1 tumors and prolong the lifetime of tumor-bearing mice.

The invention belongs to the technical field of medical testing and discloses a cerebral hemorrhage animal model combining electroacupuncture stimulation with MSCs transplanting. The cerebral hemorrhage animal model can inhibit expression of AQP-4 at the acute stage of cerebral hemorrhage, relieve local encephaledema, promote increasing of AQP-4 expression at the recovery stage, accelerate transmembrane transport of water and facilitate edema elimination, can increase AQP-4 expression after cerebral hemorrhage, accelerate elimination of encephaledema, promote recovery of cerebral energy metabolism, lower expression of MMP-9 and relieve transplanting rejective reaction. On the basis of copying a rat cerebral hemorrhage model, combined treatment through electroacupuncture stimulation and MSCs transplanting is applied, immunohistochemistry is adopted to detect expression change of AQP-4, AQP-9 and MMP-9 to analyze relation between three kinds of proteins and encephaledema evolution, and afoundation is laid for studying physiopathological mechanisms on combining electroacupuncture with cell transplanting to treat the cerebral hemorrhage.