60 results about "Antioxidative peptide" patented technology

The invention relates to the technical field of biology, in particular to multifunctional belly-expanding hippocampal peptide as well as a preparation method and application thereof. Comprising a polypeptide with an amino acid sequence as shown in SEQ ID NO.1. The hippocampus antioxidative peptide is prepared by adopting an ultrahigh pressure pretreatment and enzymolysis technology, the adopted extraction process is simple in route, and the time and the production cost are effectively saved; an animal model is utilized to prove the anti-oxidation effect and the effect of improving male reproductive disorder and testis injury of the multifunctional belly-expanding hippocampal peptide; and on the basis, a novel peptide fragment with high activity, no toxicity and no sensitization is obtained.

The invention relates to the technical field of biology, in particular to a swelling hippocampal peptide as well as a preparation method and application thereof. Comprising a polypeptide with an amino acid sequence as shown in SEQ ID NO.1. The hippocampus antioxidative peptide is prepared by adopting an ultrahigh pressure pretreatment and enzymolysis technology, the adopted extraction process is simple in route, and the time and the production cost are effectively saved; an animal model is utilized to prove the antioxidation effect and the effect of improving male reproductive disorder and testis injury of the hippocampal swelling peptide; and on the basis, a novel peptide fragment with high activity, no toxicity and no sensitization is obtained.

The invention relates to the technical field of biology, in particular to a swelling hippocampus antioxidative peptide as well as a preparation method and application thereof. Comprising a polypeptide with an amino acid sequence as shown in SEQ ID NO.1. The hippocampus antioxidative peptide is prepared by adopting an ultrahigh pressure pretreatment and enzymolysis technology, the adopted extraction process is simple in route, and the time and the production cost are effectively saved; an animal model is used for proving the antioxidation effect of the hippocampus erectus antioxidative peptide and the effect of improving male reproductive disorder and testis injury; and on the basis, a novel peptide fragment with high activity, no toxicity and no sensitization is obtained.

The invention belongs to the technical field of biology, and particularly relates to a novel antioxidant peptide and application thereof, and the amino acid sequence of the novel antioxidant peptide is shown as SEQ ID NO.1. The antioxidant peptide is high in biological activity, has relatively high scavenging capacity on DPPH. Free radicals and ABTS. Free radicals, has relatively high reducing capacity on Fe < 3 + >, and has wide application in the aspects of preparing antioxidant medicines, foods or cosmetics and the like. Meanwhile, the antioxidant peptide is short in sequence, easy to synthesize and suitable for large-scale production and application.

This invention belongs to the field of food processing technology, specifically providing a method for preparing antioxidant zinc chelate peptides, comprising the following steps: inoculating a protein solution with a compound microbial agent for fermentation; the compound microbial agent includes *Lactobacillus plantarum* and *Saccharomyces cerevisiae*; after enzymatic hydrolysis and debittering of the fermentation broth, the antioxidant peptides are separated and dried to obtain the antioxidant peptides; the antioxidant peptides are dissolved in water to prepare a peptide solution, and a zinc source is added to the peptide solution to carry out a chelation reaction; the supernatant is collected, and the precipitate is collected by alcohol precipitation to obtain the antioxidant zinc chelate peptides. This method uses low molecular weight peptides prepared by dual-microbial synergistic multi-enzyme hydrolysis as chelating ligands, which chelate with zinc ions under mild conditions to maximize the preservation of biological activity, achieving a dual enhancement of chelation capacity and antioxidant activity, and achieving a unity of high chelation rate, high antioxidant activity, high stability and good flavor. The process is green, safe, and leaves no organic solvent residue, making it suitable for large-scale industrial production.

This invention relates to the field of bioactive peptide preparation technology, and discloses a method for preparing and applying a bifunctional antioxidant and anti-inflammatory peptide based on porcine skin collagen. The bifunctional peptide has the amino acid sequence GPSGPPGEKGP. Through peptidomics and computer simulation screening, this invention identified five novel antioxidant peptides with good solubility, non-sensitizing properties, and non-toxicity. Using an AFB1-induced HuH7 cell oxidative damage model, in in vitro cell experiments, GP-11 was shown to significantly reduce ALT, AST activity, and ROS levels, enhance the activity of SOD, CAT, and GSH-Px antioxidant enzymes, and activate the Keap1-Nrf2 pathway. It also reduced the levels of inflammatory factors TNF-α and IL-6 by downregulating the classical cGAS-STING inflammatory pathway. GP-11 exhibits both significant antioxidant effects and the ability to reduce the levels of inflammatory factors, demonstrating a dual function of antioxidation and anti-inflammation.

The invention discloses a molecular docking screening method and application of hermetia illucens protein antioxidant peptides, and relates to the technical field of biologication.The method comprises the following steps that S1, hermetia illucens protein is prepared through enzymolysis, wherein hermetia illucens defatted protein powder serves as a raw material, enzymolysis is conducted through pepsin under the enzymolysis conditions that the pH is 2.0, the temperature is 40 DEG C, the enzyme adding amount is 3% of the protein mass, the enzymolysis time is 3 h, and enzymolysis liquid is obtained; after the reaction is finished, inactivating, centrifuging, collecting supernatant, and freeze-drying to obtain hermetia illucens protein hydrolysate; according to the molecular docking screening method and application of the hermetia illucens protein antioxidant peptide, the molecular docking technology is specifically applied to efficient screening of the hermetia illucens-sourced antioxidant peptide, KEAP1 is taken as a key target, high-activity candidate peptide is rapidly and accurately positioned from massive enzymolysis peptide fragments, and the screening efficiency of the hermetia illucens-sourced antioxidant peptide is improved. The defects that a traditional blind screening method is large in workload, long in period and high in cost are overcome, and the screening efficiency and the success rate are remarkably improved.

The application discloses an eggshell membrane peptide with antioxidant and improving exercise capacity of aging organism and application thereof, and belongs to the technical field of biology. The amino acid sequence of the eggshell membrane peptide is shown as SEQ ID NO. 1, and is specifically EQPWFPAG. A novel antioxidant peptide EQPWFPAG is identified from chicken eggshell membrane hydrolysate, and the eggshell membrane peptide can improve the exercise capacity and antioxidant stress capacity of an aging organism through verification. The eggshell membrane peptide provided by the application can be used for preparing an antioxidant, and can also be used for preparing functional food, health products and pharmaceutical compositions for improving the exercise function of an aging organism, and has great application value.

This invention provides an application of tartary buckwheat antioxidant peptide as a PPARα agonist. The tartary buckwheat antioxidant peptide (SEQ ID NO:1) is used to prepare a PPARα agonist, and this tartary buckwheat antioxidant peptide is used to prepare a drug for improving metabolic dysfunction-related fatty liver disease (MASLD). The tartary buckwheat antioxidant peptide of this invention provides a new drug candidate for improving MASLD and has broad clinical application prospects.

The invention relates to the technical field of biological medicine, and discloses a yak milk casein antioxidant peptide as well as a preparation method and application thereof, and the preparation method comprises the following steps: step 1, dissolving yak milk casein in alkali liquor to obtain a casein solution; step 2, carrying out enzymolysis on the casein solution by using protease to obtain enzymatic hydrolysate; step 3, inactivating and centrifuging the enzymatic hydrolysate, taking supernate, and freeze-drying to obtain a crude polypeptide product; and step 4, purifying the polypeptide crude product through anion exchange column chromatography and gel filtration chromatography in sequence to obtain the antioxidant peptide component. The preparation method is efficient and clear in target, yak milk casein is subjected to enzymolysis through a single enzyme / complex enzyme combined strategy, a two-step purification process of DEAE-52 cellulose column chromatography and SephadexG-50 gel chromatography is combined, the high-activity polypeptide component is efficiently and repeatedly prepared, and the defect that traditional enzymolysis product components are complex is overcome.

The invention relates to pressed salted duck antioxidant peptide and a preparation method thereof. The method comprises the following steps: carrying out synergistic inoculation and fermentation treatment on lactobacillus curvatus and staphylococcus mimicus, and carrying out pickling, air drying and peptide extraction processes to obtain the small molecular peptide with the molecular weight of less than 10 kDa. Through nano-LC-MS / MS (Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) detection, computer virtual screening and molecular docking, three peptide sequences, namely GIRLGLDPKL, PAPAAKAGASTGRIV and PEKPPTIDWA, with remarkable antioxidant activity are determined. According to the method, protein degradation can be effectively promoted, the yield of the antioxidant peptide is increased, and the obtained peptide has good stability and safety and is suitable for development of functional food and health care products.

The invention belongs to the technical field of bioactive peptide preparation, and discloses a preparation method of a tylorrhynchus antioxidant peptide, which comprises the following steps: mixing tylorrhynchus homogenate with enzyme, and carrying out enzymolysis to obtain the tylorrhynchus antioxidant peptide, the enzyme comprises flavourzyme and pancreatin. The invention provides the preparation method of the tylorrhynchus antioxidant peptide which is high in antioxidant activity and suitable for industrial mass production, the protein hydrolysis degree is improved, and the oxidation resistance of the tylorrhynchus antioxidant peptide is improved. The method can effectively solve the problems of low efficiency, high cost and inapplicability to large-scale extraction and preparation of the tylorrhynchus antioxidative peptide in the existing polypeptide extraction process.

The invention belongs to the technical field of biological peptides and application, and particularly relates to an antioxidant peptide with a Keap1 inhibiting effect and application of the antioxidant peptide. The antioxidant peptide provided by the invention comprises antioxidant pentapeptide, antioxidant tripeptide and the like which all show good in-vitro antioxidant activity, the scavenging rate of ABTS free radicals reaches 0.69-1.11 [mu] mol TE / [mu] mol, the scavenging rate of DPPH free radicals reaches 30.11%-34.94%, in addition, the antioxidant peptide can activate the expression of Nrf2 and downstream genes HO-1 and NQO1 thereof by inhibiting the expression level of Keap1 protein, so that the antioxidant activity of the antioxidant peptide is improved. Therefore, the compound has a wide application prospect in the fields of cosmetics, medicines, foods and the like.

Provided are gamma glutamylcysteine synthetase variants and microbial cells comprising such variants and / or expressing heterologous gamma-glutamylcysteine synthetase-glutathione synthetases, for producing increased amounts of the antioxidant peptide glutathione (GSH). Methods and compositions provided herein may be useful in baking and winemaking applications and in production of nutraceuticals, pharmaceuticals, cosmetics, plant protectants and / or animal feeds.

The present application relates to the field of food processing and biotechnology, and in particular to a glycosylated bovine bone collagen antioxidant peptide, a preparation method and application thereof. The glycosylated bovine bone collagen antioxidant peptide is prepared by enzymolysis and Maillard reaction using Yanbian yellow cattle bone as raw material, and the core antioxidant peptide sequence is shown as SEQ ID NO: 1-7. The present application uses Maillard reaction to endow the Yanbian yellow cattle bone collagen peptide with excellent caramel aroma, meat aroma and salty and fresh characteristics, and the antioxidant mechanism of the antioxidant peptide is jointly confirmed through multiscale simulation and in vitro experiment. Therefore, the present application integrates a set of high-value utilization route of bovine bone by using enzymolysis and Maillard modification, realizes the transformation of waste into treasure of slaughter by-products, and meets the green and sustainable development concept.

The invention relates to the technical field of medical dressings, in particular to a composition containing recombinant human collagen III and antioxidant peptide and application of the composition. The novel antioxidant peptide LR and the recombinant human-derived III-type collagen COL3A1 are compounded according to a certain proportion for use, and the optimal composition is selected through in-vitro antioxidant index detection and a repair experiment on UVB damaged cells. The final result shows that the composition provided by the invention can achieve the effects of resisting oxidation and repairing skin. The composition provided by the invention provides a new thought for improving the antioxidant activity and the skin repair effect of the III-type collagen.

The invention relates to the technical field of antioxidant peptide preparation, and discloses a method for preparing a casing byproduct antioxidant peptide by a microbiological method, which comprises the following steps: designing and optimizing a process flow for preparing the casing antioxidant peptide by a microbiological method, performing ultrafiltration separation and activity screening on a casing protein fermentation product, and analyzing a structure and components of the antioxidant peptide. According to the method for preparing the casing byproduct antioxidant peptide through the microbiological method, lactobacillus bulgaricus and streptococcus thermophilus are selected for compound fermentation, the protein degradation efficiency and the yield of the antioxidant peptide are remarkably improved through the synergistic effect of the two strains, and the yield of a target peptide fragment reaches 42% and is higher than that of single strain fermentation; according to the present invention, the fermentation time, the fermentation temperature, the pH value and the inoculation amount are optimized through the single factor experiment to determine the optimal fermentation condition key parameters, the high-activity peptide fragment is accurately screened by combining the ultrafiltration fractionation technology, the DPPH free radical scavenging rate is high, and is significantly superior to the existing casing protein source antioxidant peptide, and the separation purification technology is efficient and environmentally friendly, avoids the use of chemical reagents, and has good industrial application prospects. And the naturalness and safety of the product are ensured.

The application discloses application of a recombinant collagen type I-antioxidative peptide composition with an antioxidative function, and belongs to the technical field of biotechnology.The application provides a recombinant collagen type I-antioxidative peptide composition prepared by compounding a recombinant collagen type I and a schisandra antioxidant peptide.The composition has high free radical scavenging activity, and has stability and biological safety;and the antioxidative effect is better than that of single recombinant collagen type I or the schisandra antioxidant peptide.The amino acid sequence of the schisandra antioxidant peptide with antioxidative activity is FYPF, FLPW, FYLPF, ANWLPF, IFSPWL, FPFTYAMMLM, GPSFPIFI or GISWGFL.The application provides a scientific basis and technical support for development of antioxidative products in the fields of medical dressings, functional foods, skin care cosmetics and the like.

This invention provides an application of a tartary buckwheat antioxidant peptide as a PARP1 inhibitor. This tartary buckwheat antioxidant peptide (SEQ ID NO:1) is used to prepare a poly(ADP-ribose) polymerase 1 inhibitor, and is used to prepare a drug for preventing and / or improving diabetic retinopathy. The tartary buckwheat antioxidant peptide of this invention exerts multiple biological functions by inhibiting PARP1 / PAR: on the one hand, it reduces NAD... + On the one hand, it depletes nutrients, and on the other hand, it protects vascular endothelial function by inhibiting angiogenesis. This buckwheat antioxidant peptide can be used as a drug to prevent and / or improve diabetic retinopathy.

The invention discloses a medicago sativa antioxidant protein supplement and a processing method thereof, and belongs to the technical field of functional food and health care product processing. Performing targeted extraction; purifying and modifying; the stability is optimized; and preparing the dosage form. Active ingredients are efficiently reserved, the active ingredients of medicago sativa are fully reserved through the innovative extraction technology, and the product effect is ensured. High purity and high absorption: the purity and the human body absorption rate of the antioxidant peptide are improved by an advanced purification and modification process. The shelf life of the product is prolonged by a composite embedding and drug loading system, and the active ingredients are protected. The requirements of different consumption scenes are met, and the market application is widened. And the processing technology accords with ISO 22000 and FDA-GMP specifications, and the quality safety of the product is guaranteed.

The application discloses an antioxidant peptide from Apostichopus japonicus and a preparation method and application thereof, and relates to the technical field of biological medicines. The antioxidant peptide from Apostichopus japonicus has an amino acid sequence as shown in SEQ ID NO. 1. The antioxidant peptide with the amino acid sequence of LPPF is screened from the Apostichopus japonicus, and the peptide is verified to have excellent antioxidant activity and high safety, and has a good application prospect in the treatment of oxidative stress injury diseases.

The present application relates to the technical field of medical dressings, in particular to a composition containing recombinant human type III collagen and antioxidant peptides and application thereof. The present application uses a new type of antioxidant peptide LR and recombinant human type III collagen COL3A1 in a certain proportion, and selects the optimal composition through in vitro antioxidant index detection and repair experiments on UVB damaged cells. The final results show that the composition provided by the present application can achieve antioxidant and skin repair effects. The composition provided by the present application provides a new idea for improving the antioxidant activity and skin repair effect of type III collagen.

The invention discloses a method for preparing a nutritional base material with high antioxidant activity by taking wheat gluten protein powder as a raw material and application of the nutritional base material. According to the method, pancreatin with greatly improved protein yield and alkaline protease with greatly improved activity are selected to have a synergistic effect, the advantages of the pancreatin and the alkaline protease are fully played, the solid recovery rate, the protein recovery rate and the hydrolysis degree of wheat gluten protein enzymatic hydrolysate are improved, and a high-content and high-activity antioxidant peptide component is obtained; and through the Maillard reaction after ethanol purification, the antioxidant activity of the wheat gluten protein enzymatic hydrolysate is improved, and the preparation time of the peptide with high antioxidant activity is shortened. The method is simple and convenient in process operation, low in production cost, green, environment-friendly and high in raw material utilization rate, and the obtained nutritional base material has high antioxidant activity and high safety and is particularly suitable for high-value utilization of low-value resource wheat gluten protein powder.

This invention discloses an antioxidant peptide derived from deep-sea cold seeps and its applications, belonging to the fields of bioinformatics, development of microbial resources in extreme environments, and antioxidant peptide technology. The antioxidant peptide CSAOP4 obtained by this invention originates from a high-salt, low-temperature, high-pressure, oligotrophic environment, exhibiting excellent stability and activity. Adding 0.1 mg / mL of this antioxidant peptide resulted in an ABTS free radical scavenging rate of 44.45%, significantly superior to CSAOP1, CSAOP2, and CSAOP3 (ABTS free radical scavenging rates of 7.32%, 15.14%, and 27.71%, respectively). It is suitable for cosmetic production processes and can be mass-produced through solid-phase synthesis, offering simple preparation and high cost-effectiveness. This deep-sea cold seep antioxidant peptide is suitable for application in the cosmetic field, significantly extending the shelf life of cosmetics and reducing the risk of oxidative deterioration, showing broad application prospects.

This invention discloses a walnut protein octapeptide with pre-protective efficacy against alcoholic hepatocellular injury, its preparation method, and its applications. Using walnut protein hydrolysate as raw material, this invention screened and obtained a novel peptide with high antioxidant activity, HNVADPQR, with a molecular weight of 949.473 Da. This active peptide exhibits good antioxidant activity at the cellular level, with an EC50 of [missing information]. 50 The concentration was 0.0069 mg / mL, which was superior to the positive control glutathione (0.0122 mg / mL). In the ethanol injury model, the antioxidant peptide increased the survival rate of alcohol-damaged cells from 47.35% to 71.65%. These results indicate that the walnut protein-derived antioxidant peptide HNVADPQR can be developed and utilized as a potential antioxidant and hepatoprotective active ingredient.

The invention discloses a yellow water antioxidant peptide as well as a preparation method and application thereof, and belongs to the technical field of antioxidant peptides. The amino acid sequence of the antioxidant peptide is shown as any one of SEQ ID NO: 1 to SEQ ID NO: 13. The preparation method comprises the following steps: extracting protein from the white spirit byproduct yellow water, carrying out papain enzymolysis, ultrafiltration separation and gel chromatography purification, identifying a sequence through LC-MS / MS, and screening by combining a computer toxicity and activity prediction model. The invention also relates to application of the antioxidant peptide in preparation of food, cosmetics or antioxidant functional products. The method is efficient and low in cost, the obtained peptide fragment is remarkable in antioxidant activity, and a new way is provided for high-value utilization of yellow water.

The invention provides a preparation and screening method of royal jelly source antioxidant peptide based on in-vitro simulated digestion. The sequence of the antioxidant peptide is as shown in SEQ. ID. NO.1: FNYDPRY (recorded as P1). The method comprises the following steps: carrying out static in-vitro simulated digestion on MRJPs to obtain products at different digestion stages, carrying out polypeptide sequence identification by combining liquid chromatography-tandem mass spectrometry (LC-MS / MS), and further carrying out antioxidant activity prediction and safety evaluation on the identified polypeptide by utilizing bioinformatics, so as to obtain a plurality of candidate polypeptides; the candidate peptides are subjected to in-vitro antioxidant activity detection and comprehensive screening by adopting a DPPH method, an ABTS method and an FRAP method, and P1 in the screened candidate peptides is optimal under three in-vitro evaluation systems of the DPPH method, the ABTS method and the FRAP method. The antioxidant peptide can be used for preparing antioxidant functional food, health food, medicine or cosmetic compositions.

This invention relates to the field of food functional peptide preparation technology, specifically to a hepatoprotective and antioxidant peptide derived from corn yellow powder, its preparation method, and its applications. This invention discloses four polypeptides with antioxidant and hepatoprotective activities. In vitro activity assays show that all four polypeptides possess broad-spectrum free radical scavenging capabilities and protective effects against oxidative damage in HepG2 cells, with MMPGMMPPMTM exhibiting the best activity. This invention also provides a method for preparing the corn hepatoprotective peptide, employing a three-modal synergistic pretreatment process of ultrasound-high pressure homogenization-microwave, combined with synergistic fermentation by Bacillus subtilis and Bacillus licheniformis, and the addition of proline and glycine metabolism regulators, followed by continuous membrane-gel purification. This process significantly improves hydrolysis efficiency, peptide yield, and peptide content in the fermentation broth, and is complete and highly scalable. The obtained corn hepatoprotective peptide is natural, safe, and highly effective, and can be widely used in hepatoprotective health foods and functional beverages.

The invention relates to the technical field of biology, and discloses an antioxidant peptide derived from fermented meat and application of the antioxidant peptide, the antioxidant peptide comprises minced pork, auxiliary materials and a composite leavening agent composition composed of lactobacillus sake and staphylococcus xylosus, and fermentation and maturation are carried out by controlling the inoculum size and adopting a two-stage temperature control process. The core innovation of the method is as follows: firstly, performing comprehensive peptide sequence identification on small molecular weight components extracted from a fermentation product by adopting liquid chromatography-tandem mass spectrometry, and then accurately predicting and locking a target antioxidant peptide from a peptide library by utilizing virtual screening technologies such as molecular docking and the like. The antioxidant peptide prepared by the invention can be used for effectively relieving cell oxidative damage induced by ethanol by activating a Kelch sample ECH associated protein 1-Nrf2 (Keap1-Nrf2) signal channel. According to the method, directional fermentation and computational biology are combined, a new normal form is provided for efficiently discovering novel active peptides from natural products, and the research and development efficiency is effectively improved.

The invention belongs to the technical field of antioxidant peptides, and particularly relates to a laver protein source antioxidant peptide tolerant to pepsin and trypsin as well as a preparation method and application of the laver protein source antioxidant peptide. According to the invention, edible seaweed is taken as a raw material, and a Keap1-targeted antioxidant peptide is screened from seaweed protein by integrating virtual enzyme digestion, molecular docking and other methods. A cell test verifies that the tripeptide can target Keap1 to activate an antioxidant system, enhance the activity of intracellular antioxidant enzyme and remove cell reactive oxygen species (ROS), and can tolerate in-vitro degradation of pepsin and trypsin, and the absorption rate in an intestinal tract model established by human colorectal adenocarcinoma cells (Caoco-2) is 1.64%. The polypeptide has the potential of being applied to oral anti-oxidation health care products and external anti-oxidation cosmetic components, and has high medicinal economic value.