185 results about "Antioxidative peptide" patented technology

The invention relates to the technical field of biology, in particular to multifunctional belly-expanding hippocampal peptide as well as a preparation method and application thereof. Comprising a polypeptide with an amino acid sequence as shown in SEQ ID NO.1. The hippocampus antioxidative peptide is prepared by adopting an ultrahigh pressure pretreatment and enzymolysis technology, the adopted extraction process is simple in route, and the time and the production cost are effectively saved; an animal model is utilized to prove the anti-oxidation effect and the effect of improving male reproductive disorder and testis injury of the multifunctional belly-expanding hippocampal peptide; and on the basis, a novel peptide fragment with high activity, no toxicity and no sensitization is obtained.

The invention discloses sea cucumber gonad-derived antioxidant peptide as well as a preparation method and application thereof, and belongs to the technical field of food biology. The antioxidant peptide is obtained by carrying out enzymolysis and molecular docking screening on sea cucumber gonads, and comprises at least one of peptides with amino acid sequences as shown in SEQ.ID.NO.1-4. Wherein the docking energy of the SEQ.ID.NO.1 and the receptor SOD is-7.5 kcal / mol, the docking energy of the SEQ.ID.NO.2 and the receptor SOD is-7.5 kcal / mol, the docking energy of the SEQ.ID.NO.3 and the receptor SOD is-7.4 kcal / mol, and the docking energy of the SEQ.ID.NO.4 and the receptor SOD is-7.2 kcal / mol. Tests prove that the antioxidant peptide has high antioxidant activity and has wide application space in the field of functional food.

The invention discloses a method for preparing, enriching and separating various egg yolk bioactive peptides through enzymolysis of defatted egg yolk powder and application of the egg yolk bioactive peptides, and belongs to the technical field of egg product processing and bioactive peptides. Egg yolk protein hydrolysate is prepared by adopting a three-step enzymolysis process, adsorption and gradient elution are performed on the egg yolk protein hydrolysate by adopting a macroporous resin chromatographic column, four components including water-1, water-2, 20% ethanol and 80% ethanol are obtained, the water-1 component is common egg yolk peptide, the water-1 component is enriched with osteogenic active peptide, the water-2 component is enriched with ethanol, and the 80% ethanol is enriched with ethanol. The 20% ethanol component enriches antioxidant peptide, and the 80% ethanol component enriches ACE inhibitory peptide. The functional activity of each bioactive peptide component is very strong, and the bioactive peptide component can be used as a high-end functional ingredient. According to the method disclosed by the invention, comprehensive utilization of the degreased yolk powder is realized, enrichment and separation of three active peptides are realized by using one raw material through a step-by-step enzymolysis-directional separation technology, the yield of the active peptides is greatly improved, and the economic benefits of enterprises are improved.

The invention relates to the technical field of bioactive peptides, in particular to an antioxidant peptide based on mussel byssus, a preparation method, a preparation and application of the antioxidant peptide based on mussel byssus, the antioxidant peptide is designed and synthesized by referring to the structural characteristics of mussel byssus proteins mfp-3, mfp-5 and mfp-6, the antioxidant peptide comprises four sequences of MFP3-AP1, MFP5-AP2, MFP6-AP3 and MFP-Hybrid, tyrosine in the peptide is modified into DOPA through enzymatic modification, and the antioxidant peptide is prepared through enzymatic modification. The stability and activity of the antioxidant peptide are enhanced through disulfide bond formation, metal ion coordination, PEGylation and other modifications, and the antioxidant peptide has high DPPH free radical scavenging activity, ABTS free radical scavenging activity, hydroxyl free radical scavenging activity and iron ion reducing capacity, has a protective effect on the cellular level, can be prepared into a liquid preparation, a freeze-dried preparation or a micro-capsule preparation, and can be used for preparing the antioxidant peptide. The compound is expected to be applied to the fields of antioxidant health care products, cosmetics and medicines.

The invention relates to the technical field of biology, in particular to a swelling hippocampal peptide as well as a preparation method and application thereof. Comprising a polypeptide with an amino acid sequence as shown in SEQ ID NO.1. The hippocampus antioxidative peptide is prepared by adopting an ultrahigh pressure pretreatment and enzymolysis technology, the adopted extraction process is simple in route, and the time and the production cost are effectively saved; an animal model is utilized to prove the antioxidation effect and the effect of improving male reproductive disorder and testis injury of the hippocampal swelling peptide; and on the basis, a novel peptide fragment with high activity, no toxicity and no sensitization is obtained.

The invention relates to the technical field of bioactive peptides, and particularly discloses a peptide oxidation resistance prediction method and system based on a pre-training model and descriptors, and the method comprises the following steps: obtaining an amino acid sequence of a to-be-predicted polypeptide and a physicochemical characteristic description index of each amino acid; enabling the coded amino acid sequence to pass through a BERT embedding layer to form high-dimensional vector representation, inputting the high-dimensional vector representation into a pre-trained deep neural network model to obtain features of a peptide sequence, and inputting the features of the peptide sequence into a pre-trained artificial neural network to obtain deep representation of the peptide sequence; according to the physicochemical characteristic description index of each amino acid, generating a characteristic vector of each amino acid, and constructing the characteristic of the polypeptide; and inputting the depth representation of the peptide sequence and the characteristics of the polypeptide into a predictor to obtain a prediction result of the oxidation resistance of the peptide. According to the method, the fine-tuned model characterization is combined with the traditional peptide sequence descriptor, so that the prediction accuracy of the antioxidant peptide and the stability of the model are successfully improved.

The invention relates to the technical field of preparation methods of giant salamander peptide and eucommia ulmoides male flower tablets, in particular to a preparation method of giant salamander peptide and eucommia ulmoides male flower tablets. The giant salamander peptide and eucommia ulmoides male flower tablets comprise the following raw materials in parts by weight: 30-40 parts of giant salamander peptide powder, 20-25 parts of eucommia ulmoides male flowers, 10-15 parts of rape pollen, 10-15 parts of sea cucumber intestine peptide powder, 10-12 parts of a filler, 3-5 parts of an adhesive and 1-3 parts of a lubricant. According to the invention, the eucommia male flower contains geniposidic acid, chlorogenic acid, aucubin lignin, iridoid and phenylpropanoid natural active nutrient substances, flavonoid compounds, polysaccharide and other bioactive substances in the rape pollen, polysaccharide and peptide components in the sea cucumber intestine peptide powder, and the like. The andrias davidianus peptide powder is added into the andrias davidianus peptide powder, the andrias davidianus peptide powder cooperates with antioxidant peptide and immunomodulatory peptide in the andrias davidianus peptide powder to jointly form a rich bioactive substance library, and the components regulate the physiological functions of the human body from different angles, provide comprehensive nutrition support for the body and improve the fatigue resistance of the human body.

The invention discloses a method for auxiliary screening of grease antioxidant peptides based on artificial intelligence, and belongs to the technical field of bioinformatics. According to the invention, deep learning and virtual enzymolysis are combined, so that a novel method for preparing the antioxidant peptide by rapidly and efficiently screening protein enzymolysis is researched and developed. According to the method, an antioxidant peptide is obtained as a target, an antioxidant peptide data set and a test set are constructed, a Bi-LSTM high-precision deep learning strategy is provided based on the data set, and the Bi-LSTM high-precision deep learning strategy is trained to recognize the antioxidant peptide. And then, predicting the antioxidant activity of the polypeptide obtained through virtual enzymolysis by utilizing Bi-LSTM (Bidirectional Long Short Term Memory). Taking black bean protein as an example, the strategy of the invention discovers that the antioxidant peptide can be better obtained by enzymolysis of the black bean protein through trypsin. After ABTS and DPPH activity verification, the antioxidant peptide is used for lipophilicity determination, the peptide with lipophilicity can play a better grease antioxidant effect, and a research basis is provided for safety control of a grease hot working process.

To provide antioxidant peptides derived from Xuanwei ham, and to provide methods for preparation and activity measurement thereof.SOLUTION: The present invention provides an antioxidant peptide with a specific amino acid sequence derived from Xuanwei ham. The present invention uses a rigid linking peptide and a flexible linking peptide to link a hexapeptide derived from Xuanwei ham, growing the peptide chain to make it suitable for genetic recombinant expression, while increasing the proportion of a target polypeptide in a recombinant protein and significantly increasing the expression level. The rigid linking peptide has an α-helical structure, which makes it easier for the antioxidant peptide to form a stable secondary structure after serial linking, and can effectively separate the active functional domain.The flexible linking peptide is mainly composed of glycine Gly, and since it has a low molecular weight, the flexibility of a polypeptide backbone structure is ensured.SELECTED DRAWING: Figure 1

The invention belongs to the technical field of food biology, and particularly relates to an antioxidant peptide derived from cantharellus cibarius sporocarp, an antioxidant peptide detection reagent and application, and the amino acid sequence of the antioxidant peptide is at least one of FPYPY, YPPYF, YYPF and MPWY. The antioxidant peptide provided by the invention is derived from chanterelle, is an enzymolysis product of chanterelle protein, and is derived from edible and medicinal fungus chanterelle, so that the antioxidant peptide is small in toxic and side effects and high in safety. Meanwhile, the antioxidant peptide disclosed by the invention has good biological activity and a relatively strong antioxidant effect, can remarkably scavenge free radicals, and can be applied to repair damage caused by oxidative stress.

The invention discloses a high-throughput screening method and device for antioxidant polypeptides, and the method comprises the following steps: (1) collecting polypeptides to construct a polypeptide data set which comprises antioxidant peptides, non-antioxidant peptides and random peptides; (2) encoding polypeptides in the polypeptide data set, extracting features, forming vector representation, and dividing into a training set and a test set; (3) training the learning model by adopting the training set and the test set to obtain a prediction model of the antioxidant polypeptide; and (4) substituting to-be-screened polypeptide sequences into the prediction model in batches, and outputting the antioxidant probability of the polypeptide by the prediction model. According to the method and the device, the oxidation resistance of the polypeptide can be quickly predicted in a high-throughput manner, and meanwhile, relatively high accuracy and a model Morse correlation coefficient are ensured.

The invention relates to the technical field of biological agents, in particular to abalone-derived polypeptide with antioxidant activity and a preparation method thereof. According to the invention, Haliotis discus hannai Ino is taken as a research object, a small peptide library is established by using a virtual enzymolysis technology from an important anti-oxidation pathway of cells, namely a Keap1-Nrf2 pathway, an abalone-derived anti-oxidation peptide DEDEDEDK with relatively strong binding capacity with Keap1 is screened out by using a molecular docking technology, and then, the anti-oxidation activity of the abalone-derived anti-oxidation peptide DEDEDEDK is verified by using a cell oxidative damage model, so that the abalone-derived anti-oxidation peptide DEDEDEDK can be used for preparing the anti-oxidation peptide DEDEDEDK with relatively strong binding capacity with Keap1. And a theoretical basis is provided for research and application of the antioxidant peptide.

The invention relates to the technical field of biology, in particular to a swelling hippocampus antioxidative peptide as well as a preparation method and application thereof. Comprising a polypeptide with an amino acid sequence as shown in SEQ ID NO.1. The hippocampus antioxidative peptide is prepared by adopting an ultrahigh pressure pretreatment and enzymolysis technology, the adopted extraction process is simple in route, and the time and the production cost are effectively saved; an animal model is used for proving the antioxidation effect of the hippocampus erectus antioxidative peptide and the effect of improving male reproductive disorder and testis injury; and on the basis, a novel peptide fragment with high activity, no toxicity and no sensitization is obtained.

The invention belongs to the technical field of food biology, and particularly relates to a sea cucumber-derived antioxidant peptide as well as a preparation method and application thereof. The sea cucumber source antioxidant peptide is prepared and screened from apostichopus japonicus and comprises at least one of peptides with the amino acid sequence shown as SEQ ID NO.1-4, and the docking energy of the peptides and a protein receptor Keap1 is-8.6 kcal / mol,-9.4 kcal / mol,-9.1 kcal / mol and-8.8 kcal / mol respectively. The antioxidant peptide obtained by the invention can be applied to food, cosmetics or medicines, and has important significance on the development of novel antioxidant products.

The invention discloses a collagen peptide, the amino acid sequence of which comprises GPMGF, GPPGLPGF, GPPGPLGLL, GPPGAPGPLGI, GPPGPLGL, GPPGLPGL, LGPGGF, FGPGYGP, AGPAGPAGPR, GFPGYPQ, GISV PGPMGPSGPRG, GLPGVPGPGFP, PGPVGPSGP, ISV PGPMGPSGPRG, ISP GPPGPGGP, APGMPGSPGPA, APGMPGSPGPA, GPAGLPGVPG, PGLLPGPGPA, and PGIPGPA. The amino acid sequence of the collagen peptide comprises GPMGF, the invention provides 26 polypeptides with high activity and antioxidant potential, which can be used for further screening single antioxidant peptides or anti-aging peptides and developing functional skin care products or other antioxidant products.

The invention discloses a polypeptide FADHPG and application of the polypeptide FADHPG in preparation of an anti-freezing and anti-oxidation preparation, and belongs to the technical field of bioactive peptides. The amino acid sequence of the polypeptide FADHPG is FADHPG, and the amino acid sequence of the polypeptide FADHPG is as shown in SEQ ID NO. 1. The invention also discloses an application of the polypeptide FADHPG in preparation of an anti-freezing and anti-oxidation preparation. The novel antifreeze antioxidant peptide is excavated from litopenaeus vannamei protein through a dual deep learning method, and experimental analysis verifies that the average particle size of ice crystals treated by the polypeptide FADHPG is reduced by 50% or above, the free radical scavenging capacity reaches 47%, and the effect is excellent. The invention provides a new raw material basis for the development of a novel efficient composite multifunctional anti-freezing antioxidant, and has a good application prospect and an important application value.