185 results about "Antioxidative peptide" patented technology

The invention relates to the technical field of biology, in particular to multifunctional belly-expanding hippocampal peptide as well as a preparation method and application thereof. Comprising a polypeptide with an amino acid sequence as shown in SEQ ID NO.1. The hippocampus antioxidative peptide is prepared by adopting an ultrahigh pressure pretreatment and enzymolysis technology, the adopted extraction process is simple in route, and the time and the production cost are effectively saved; an animal model is utilized to prove the anti-oxidation effect and the effect of improving male reproductive disorder and testis injury of the multifunctional belly-expanding hippocampal peptide; and on the basis, a novel peptide fragment with high activity, no toxicity and no sensitization is obtained.

The invention discloses sea cucumber gonad-derived antioxidant peptide as well as a preparation method and application thereof, and belongs to the technical field of food biology. The antioxidant peptide is obtained by carrying out enzymolysis and molecular docking screening on sea cucumber gonads, and comprises at least one of peptides with amino acid sequences as shown in SEQ.ID.NO.1-4. Wherein the docking energy of the SEQ.ID.NO.1 and the receptor SOD is-7.5 kcal / mol, the docking energy of the SEQ.ID.NO.2 and the receptor SOD is-7.5 kcal / mol, the docking energy of the SEQ.ID.NO.3 and the receptor SOD is-7.4 kcal / mol, and the docking energy of the SEQ.ID.NO.4 and the receptor SOD is-7.2 kcal / mol. Tests prove that the antioxidant peptide has high antioxidant activity and has wide application space in the field of functional food.

The invention discloses a method for preparing, enriching and separating various egg yolk bioactive peptides through enzymolysis of defatted egg yolk powder and application of the egg yolk bioactive peptides, and belongs to the technical field of egg product processing and bioactive peptides. Egg yolk protein hydrolysate is prepared by adopting a three-step enzymolysis process, adsorption and gradient elution are performed on the egg yolk protein hydrolysate by adopting a macroporous resin chromatographic column, four components including water-1, water-2, 20% ethanol and 80% ethanol are obtained, the water-1 component is common egg yolk peptide, the water-1 component is enriched with osteogenic active peptide, the water-2 component is enriched with ethanol, and the 80% ethanol is enriched with ethanol. The 20% ethanol component enriches antioxidant peptide, and the 80% ethanol component enriches ACE inhibitory peptide. The functional activity of each bioactive peptide component is very strong, and the bioactive peptide component can be used as a high-end functional ingredient. According to the method disclosed by the invention, comprehensive utilization of the degreased yolk powder is realized, enrichment and separation of three active peptides are realized by using one raw material through a step-by-step enzymolysis-directional separation technology, the yield of the active peptides is greatly improved, and the economic benefits of enterprises are improved.

The invention relates to the technical field of bioactive peptides, in particular to an antioxidant peptide based on mussel byssus, a preparation method, a preparation and application of the antioxidant peptide based on mussel byssus, the antioxidant peptide is designed and synthesized by referring to the structural characteristics of mussel byssus proteins mfp-3, mfp-5 and mfp-6, the antioxidant peptide comprises four sequences of MFP3-AP1, MFP5-AP2, MFP6-AP3 and MFP-Hybrid, tyrosine in the peptide is modified into DOPA through enzymatic modification, and the antioxidant peptide is prepared through enzymatic modification. The stability and activity of the antioxidant peptide are enhanced through disulfide bond formation, metal ion coordination, PEGylation and other modifications, and the antioxidant peptide has high DPPH free radical scavenging activity, ABTS free radical scavenging activity, hydroxyl free radical scavenging activity and iron ion reducing capacity, has a protective effect on the cellular level, can be prepared into a liquid preparation, a freeze-dried preparation or a micro-capsule preparation, and can be used for preparing the antioxidant peptide. The compound is expected to be applied to the fields of antioxidant health care products, cosmetics and medicines.

The invention relates to the technical field of biology, in particular to a swelling hippocampal peptide as well as a preparation method and application thereof. Comprising a polypeptide with an amino acid sequence as shown in SEQ ID NO.1. The hippocampus antioxidative peptide is prepared by adopting an ultrahigh pressure pretreatment and enzymolysis technology, the adopted extraction process is simple in route, and the time and the production cost are effectively saved; an animal model is utilized to prove the antioxidation effect and the effect of improving male reproductive disorder and testis injury of the hippocampal swelling peptide; and on the basis, a novel peptide fragment with high activity, no toxicity and no sensitization is obtained.

The invention relates to the technical field of bioactive peptides, and particularly discloses a peptide oxidation resistance prediction method and system based on a pre-training model and descriptors, and the method comprises the following steps: obtaining an amino acid sequence of a to-be-predicted polypeptide and a physicochemical characteristic description index of each amino acid; enabling the coded amino acid sequence to pass through a BERT embedding layer to form high-dimensional vector representation, inputting the high-dimensional vector representation into a pre-trained deep neural network model to obtain features of a peptide sequence, and inputting the features of the peptide sequence into a pre-trained artificial neural network to obtain deep representation of the peptide sequence; according to the physicochemical characteristic description index of each amino acid, generating a characteristic vector of each amino acid, and constructing the characteristic of the polypeptide; and inputting the depth representation of the peptide sequence and the characteristics of the polypeptide into a predictor to obtain a prediction result of the oxidation resistance of the peptide. According to the method, the fine-tuned model characterization is combined with the traditional peptide sequence descriptor, so that the prediction accuracy of the antioxidant peptide and the stability of the model are successfully improved.

The invention relates to the technical field of preparation methods of giant salamander peptide and eucommia ulmoides male flower tablets, in particular to a preparation method of giant salamander peptide and eucommia ulmoides male flower tablets. The giant salamander peptide and eucommia ulmoides male flower tablets comprise the following raw materials in parts by weight: 30-40 parts of giant salamander peptide powder, 20-25 parts of eucommia ulmoides male flowers, 10-15 parts of rape pollen, 10-15 parts of sea cucumber intestine peptide powder, 10-12 parts of a filler, 3-5 parts of an adhesive and 1-3 parts of a lubricant. According to the invention, the eucommia male flower contains geniposidic acid, chlorogenic acid, aucubin lignin, iridoid and phenylpropanoid natural active nutrient substances, flavonoid compounds, polysaccharide and other bioactive substances in the rape pollen, polysaccharide and peptide components in the sea cucumber intestine peptide powder, and the like. The andrias davidianus peptide powder is added into the andrias davidianus peptide powder, the andrias davidianus peptide powder cooperates with antioxidant peptide and immunomodulatory peptide in the andrias davidianus peptide powder to jointly form a rich bioactive substance library, and the components regulate the physiological functions of the human body from different angles, provide comprehensive nutrition support for the body and improve the fatigue resistance of the human body.

The invention discloses a method for auxiliary screening of grease antioxidant peptides based on artificial intelligence, and belongs to the technical field of bioinformatics. According to the invention, deep learning and virtual enzymolysis are combined, so that a novel method for preparing the antioxidant peptide by rapidly and efficiently screening protein enzymolysis is researched and developed. According to the method, an antioxidant peptide is obtained as a target, an antioxidant peptide data set and a test set are constructed, a Bi-LSTM high-precision deep learning strategy is provided based on the data set, and the Bi-LSTM high-precision deep learning strategy is trained to recognize the antioxidant peptide. And then, predicting the antioxidant activity of the polypeptide obtained through virtual enzymolysis by utilizing Bi-LSTM (Bidirectional Long Short Term Memory). Taking black bean protein as an example, the strategy of the invention discovers that the antioxidant peptide can be better obtained by enzymolysis of the black bean protein through trypsin. After ABTS and DPPH activity verification, the antioxidant peptide is used for lipophilicity determination, the peptide with lipophilicity can play a better grease antioxidant effect, and a research basis is provided for safety control of a grease hot working process.

To provide antioxidant peptides derived from Xuanwei ham, and to provide methods for preparation and activity measurement thereof.SOLUTION: The present invention provides an antioxidant peptide with a specific amino acid sequence derived from Xuanwei ham. The present invention uses a rigid linking peptide and a flexible linking peptide to link a hexapeptide derived from Xuanwei ham, growing the peptide chain to make it suitable for genetic recombinant expression, while increasing the proportion of a target polypeptide in a recombinant protein and significantly increasing the expression level. The rigid linking peptide has an α-helical structure, which makes it easier for the antioxidant peptide to form a stable secondary structure after serial linking, and can effectively separate the active functional domain.The flexible linking peptide is mainly composed of glycine Gly, and since it has a low molecular weight, the flexibility of a polypeptide backbone structure is ensured.SELECTED DRAWING: Figure 1

The invention belongs to the technical field of food biology, and particularly relates to an antioxidant peptide derived from cantharellus cibarius sporocarp, an antioxidant peptide detection reagent and application, and the amino acid sequence of the antioxidant peptide is at least one of FPYPY, YPPYF, YYPF and MPWY. The antioxidant peptide provided by the invention is derived from chanterelle, is an enzymolysis product of chanterelle protein, and is derived from edible and medicinal fungus chanterelle, so that the antioxidant peptide is small in toxic and side effects and high in safety. Meanwhile, the antioxidant peptide disclosed by the invention has good biological activity and a relatively strong antioxidant effect, can remarkably scavenge free radicals, and can be applied to repair damage caused by oxidative stress.

The invention discloses a high-throughput screening method and device for antioxidant polypeptides, and the method comprises the following steps: (1) collecting polypeptides to construct a polypeptide data set which comprises antioxidant peptides, non-antioxidant peptides and random peptides; (2) encoding polypeptides in the polypeptide data set, extracting features, forming vector representation, and dividing into a training set and a test set; (3) training the learning model by adopting the training set and the test set to obtain a prediction model of the antioxidant polypeptide; and (4) substituting to-be-screened polypeptide sequences into the prediction model in batches, and outputting the antioxidant probability of the polypeptide by the prediction model. According to the method and the device, the oxidation resistance of the polypeptide can be quickly predicted in a high-throughput manner, and meanwhile, relatively high accuracy and a model Morse correlation coefficient are ensured.

The invention relates to the technical field of biological agents, in particular to abalone-derived polypeptide with antioxidant activity and a preparation method thereof. According to the invention, Haliotis discus hannai Ino is taken as a research object, a small peptide library is established by using a virtual enzymolysis technology from an important anti-oxidation pathway of cells, namely a Keap1-Nrf2 pathway, an abalone-derived anti-oxidation peptide DEDEDEDK with relatively strong binding capacity with Keap1 is screened out by using a molecular docking technology, and then, the anti-oxidation activity of the abalone-derived anti-oxidation peptide DEDEDEDK is verified by using a cell oxidative damage model, so that the abalone-derived anti-oxidation peptide DEDEDEDK can be used for preparing the anti-oxidation peptide DEDEDEDK with relatively strong binding capacity with Keap1. And a theoretical basis is provided for research and application of the antioxidant peptide.

The invention relates to the technical field of biology, in particular to a swelling hippocampus antioxidative peptide as well as a preparation method and application thereof. Comprising a polypeptide with an amino acid sequence as shown in SEQ ID NO.1. The hippocampus antioxidative peptide is prepared by adopting an ultrahigh pressure pretreatment and enzymolysis technology, the adopted extraction process is simple in route, and the time and the production cost are effectively saved; an animal model is used for proving the antioxidation effect of the hippocampus erectus antioxidative peptide and the effect of improving male reproductive disorder and testis injury; and on the basis, a novel peptide fragment with high activity, no toxicity and no sensitization is obtained.

The invention belongs to the technical field of food biology, and particularly relates to a sea cucumber-derived antioxidant peptide as well as a preparation method and application thereof. The sea cucumber source antioxidant peptide is prepared and screened from apostichopus japonicus and comprises at least one of peptides with the amino acid sequence shown as SEQ ID NO.1-4, and the docking energy of the peptides and a protein receptor Keap1 is-8.6 kcal / mol,-9.4 kcal / mol,-9.1 kcal / mol and-8.8 kcal / mol respectively. The antioxidant peptide obtained by the invention can be applied to food, cosmetics or medicines, and has important significance on the development of novel antioxidant products.

The invention discloses a collagen peptide, the amino acid sequence of which comprises GPMGF, GPPGLPGF, GPPGPLGLL, GPPGAPGPLGI, GPPGPLGL, GPPGLPGL, LGPGGF, FGPGYGP, AGPAGPAGPR, GFPGYPQ, GISV PGPMGPSGPRG, GLPGVPGPGFP, PGPVGPSGP, ISV PGPMGPSGPRG, ISP GPPGPGGP, APGMPGSPGPA, APGMPGSPGPA, GPAGLPGVPG, PGLLPGPGPA, and PGIPGPA. The amino acid sequence of the collagen peptide comprises GPMGF, the invention provides 26 polypeptides with high activity and antioxidant potential, which can be used for further screening single antioxidant peptides or anti-aging peptides and developing functional skin care products or other antioxidant products.

The invention discloses a polypeptide FADHPG and application of the polypeptide FADHPG in preparation of an anti-freezing and anti-oxidation preparation, and belongs to the technical field of bioactive peptides. The amino acid sequence of the polypeptide FADHPG is FADHPG, and the amino acid sequence of the polypeptide FADHPG is as shown in SEQ ID NO. 1. The invention also discloses an application of the polypeptide FADHPG in preparation of an anti-freezing and anti-oxidation preparation. The novel antifreeze antioxidant peptide is excavated from litopenaeus vannamei protein through a dual deep learning method, and experimental analysis verifies that the average particle size of ice crystals treated by the polypeptide FADHPG is reduced by 50% or above, the free radical scavenging capacity reaches 47%, and the effect is excellent. The invention provides a new raw material basis for the development of a novel efficient composite multifunctional anti-freezing antioxidant, and has a good application prospect and an important application value.

The invention discloses a preparation method of a dairy cow placenta extract CP1 and application of the dairy cow placenta extract CP1 as an antioxidant substance. The preparation method comprises the following steps: taking normal delivery healthy Chinese Holstein dairy cow placenta homogenate as a substrate, shearing the dairy cow placenta into pieces, homogenizing by adopting a tissue homogenizer, adding papain, and acting for 4.7 hours under the conditions that the pH is 6.5 and the temperature is 55 DEG C; the antioxidative peptide has the advantages that the antioxidative peptide with five potential types is screened by the aid of virtual screening processes after the antioxidative peptide is inactivated in boiling water bath for 10 minutes, affinity of the antioxidative peptide with Keap1 after molecular docking is higher than that of TX6, the affinity of ANNGKQWAEVF with Keap1 is the strongest, accordingly, the antioxidative peptide is marked as CP1, the CP1 is excellent in free radical scavenging capacity, lipid peroxidation inhibition capacity, transition metal chelating capacity and reducing power, and the antioxidative peptide with the antioxidative peptide can be used for preparing antioxidative peptide products. In addition, a Keap1 / Nrf2 pathway in oxidative stress cells can be activated, so that the oxidative stress of the cells is relieved, and the compound can be applied as an antioxidant substance.

The invention belongs to the technical field of food processing, and particularly relates to a method for synergistically extracting dogwood seed oil and antioxidant peptide by coupling supercritical CO2 extraction with deep-eutectic solvent DES. The method comprises the following steps: S1, performing microwave treatment on dogwood seeds, crushing and sieving to obtain dogwood seed powder; s2, the dogwood seed powder is subjected to supercritical CO2 extraction, a eutectic solvent serves as an entrainer, and residues A and dogwood seed oil are obtained after extraction is completed; s3, mixing the residue A with a deep eutectic solvent, and carrying out secondary extraction to obtain a residue B and a filtrate; s4, adding water into the filtrate, uniformly mixing, centrifuging, respectively collecting a water phase and an oil phase, carrying out reduced pressure distillation on the oil phase, and combining with the dogwood seed oil obtained in the step S2; and S5, adding the residue B obtained in the step S3 into water, and adding mixed enzyme to carry out enzymolysis reaction, so as to obtain the dogwood seed antioxidant peptide. According to the method, the dogwood seed oil and the antioxidant peptide can be extracted at the same time, the oil extraction rate of the dogwood seed oil reaches 90% or above, the yield of the antioxidant peptide is 78.7%, and the resource utilization rate is increased.

The invention discloses a yak milk-derived antioxidant peptide and a screening method and application thereof, and belongs to the technical field of bioactive peptides, the yak milk-derived antioxidant peptide is DGGY, the amino acid sequence of the yak milk-derived antioxidant peptide is shown as SEQ ID NO.1, the molecular formula of the yak milk-derived antioxidant peptide is C17H22N4O8, the chemical structural formula of the yak milk-derived antioxidant peptide is # imgabs0, and the invention further discloses the screening method and application of the yak milk-derived antioxidant peptide. The DGGY with the amino acid sequence as shown in SEQ ID NO.1 is proposed for the first time, has good antioxidant activity, is high in hydroxyl radical and ABTS free radical scavenging activity, is derived from yak milk, belongs to a natural antioxidant, is good in safety, can be used for preparing antioxidant products, and has wide application prospects.

Provided in the present invention are a sturgeon extract rich in antioxidant peptides, and a preparation method therefor and the use thereof. The sturgeon extract of the present invention comprises an amino acid, polypeptide and protein from sturgeon meat and / or roe. The total amount of the amino acid, polypeptide and protein is 20% or more based on the dry matter content. On the basis that the total mass of the amino acid, polypeptide and protein is 100%, the molecular weight distribution of the amino acid, polypeptide and protein is as follows: the content of the component with a molecular weight less than 500 Da is 9%-60%; the content of the component with a molecular weight of 500 Da-1000 Da is 10%-30%; the content of the component with a molecular weight of 1000 Da-5000 Da is 5%-60%; and the content of the component with a molecular weight greater than 5000 Da is 5%-52%. The sturgeon extract of the present invention is rich in a variety of antioxidant peptides, has a relatively high antioxidant activity and also has the functions of protecting the liver and enhancing immunity.

The invention provides a sturgeon extract rich in antioxidant peptide as well as a preparation method and application of the sturgeon extract. The sturgeon extract provided by the invention comprises amino acids, polypeptides and proteins from sturgeon meat and / or roes; the total amount of amino acid, polypeptide and protein is more than 20% by dry matter content. Wherein the total mass of the amino acid, the polypeptide and the protein is 100%, and the molecular weight distribution of the amino acid, the polypeptide and the protein is as follows: the molecular weight is less than 500Da, and the content of the amino acid, the polypeptide and the protein is 9-60%; the molecular weight is 500 Da to 1000 Da, and the content is 10 to 30 percent; the molecular weight is 1000 Da to 5000 Da, and the content is 5 to 60 percent; the molecular weight is greater than 5000Da, and the content is 5-52%. The sturgeon extract disclosed by the invention is rich in various antioxidant peptides, and has the functions of protecting liver and enhancing immunity while having relatively high antioxidant activity.

The invention provides an antioxidant peptide as well as a preparation method and application thereof, and belongs to the technical field of biology. The invention provides an antioxidant peptide with an amino acid sequence as shown in SEQ ID NO. 2 and / or an antioxidant peptide with an amino acid sequence as shown in SEQ ID NO. 4. Two antioxidative peptides are identified from the gastrodia elata protein hydrolysate through enzymolysis, ultrafiltration, gel chromatography purification and mass spectrometry technologies in combination with ABTS and DPPH free radical scavenging activity, the molecular weights of the two antioxidative peptides are 937.08 Da and 1153.50 Da respectively, and the two antioxidative peptides have high antioxidative activity, heat stability and in-vitro safety and can be used for preparing antioxidative peptides for the gastrodia elata protein hydrolysate. The cell viability decline and ROS level rise caused by oxidative stress injury of cells can be effectively relieved, and the neuroprotective effect can be achieved by activating an Nrf2 pathway. The antioxidant peptide is suitable for daily chemical products, skin care and beauty cosmetics, health foods and medicines with antioxidant efficacy requirements.

The invention relates to the technical field of antioxidant peptides, in particular to an antioxidant peptide HYHE derived from dietary protein and application of the antioxidant peptide HYHE. The amino acid sequence of the antioxidant peptide is HYHE and is as shown in SEQ ID NO: 1. The antioxidant peptide is obtained based on prediction of an antioxidant peptide prediction platform Pred5AOP; 27 dietary proteins are virtually hydrolyzed through trypsin and pepsin, and the antioxidant peptide HYHE is screened out; compared with chemical drugs, the antioxidant peptide HYHE serving as the biological peptide has the advantages of high stability, low immunogenicity, high purity and the like; meanwhile, the invention further proves that the dietary protein has rich nutritional value, provides a new thought for resource recycling, and has good social and economic benefits.

The invention provides a peptide beverage capable of dispelling effects of alcohol, protecting liver and relieving fatigue and a preparation method and application thereof.The peptide beverage comprises hovenia dulcis thunb oligopeptide, walnut peptide, antioxidant peptide, energy release auxiliary peptide and the like, and the antioxidant peptide is prepared by mixing cordyceps militaris, sea-buckthorn and sunflower heads. The energy release auxiliary peptide is prepared from carnosine and arginine succinic acid peptide through microbial fermentation. The preparation method comprises the following steps: mixing the components according to a certain ratio, stirring, homogenizing, sterilizing and the like. The peptide beverage has the effects of dispelling the effects of alcohol, protecting the liver and relieving fatigue, and is suitable for preparing drugs or health-care products for dispelling the effects of alcohol and protecting the liver. The preparation method has the characteristics of strict raw material selection and fine preparation process, and ensures the quality and efficacy of the product. Through a scientific ratio and an advanced preparation technology, the peptide beverage provides a new choice for dispelling the effects of alcohol, protecting the liver and relieving fatigue, and has a wide market application prospect.

The invention relates to the technical field of biology, in particular to antioxidative peptide and ACE (angiotensin converting enzyme) inhibitory peptide from tuna as well as a preparation method and application of the antioxidative peptide and the ACE inhibitory peptide. The amino acid sequences of the polypeptide provided by the invention are as shown in SEQ ID NO.1-6. The preparation method comprises the following steps: by taking processing waste tuna skin as a raw material, carrying out enzymolysis to obtain tuna skin collagen, then carrying out liquid chromatography-tandem mass spectrometry identification and molecular docking screening on a tuna skin collagen zymolyte, and extracting to obtain polypeptide with antioxidant activity and polypeptide capable of effectively inhibiting ACE (Angiotensin Converting Enzyme) activity. According to the method, the protein extraction step is simplified; meanwhile, on one hand, the deep processing level of the tuna industry can be improved, high-value utilization is achieved, and on the other hand, the bearing pressure of the environment on the processing waste can be reduced.

The invention relates to the field of medicines and health foods, in particular to an artichoke antioxidant peptide for protecting liver and improving stomach and intestine and a preparation method thereof, the artichoke antioxidant peptide is obtained by extracting after temperature stress treatment, and a bioactive peptide fragment is selectively extracted by adopting a trypsin enzymolysis method. The artichoke antioxidant peptide disclosed by the invention can be used for developing medicines and functional foods for treating liver diseases, and is helpful for scavenging free radicals, protecting liver cells and enhancing the antioxidant capacity of a body. In addition, the artichoke antioxidant peptide disclosed by the invention can be used as a supplement and a functional food to regulate intestinal flora, so that the overall gastrointestinal health is improved.

The invention provides an enteromorpha protein source antioxidant peptide as well as a preparation method and application thereof. Edible green algae enteromorpha is used as a raw material, the Keap1-targeted antioxidant peptide is screened from enteromorpha protein through methods of virtual enzymolysis, molecular docking, molecular dynamics simulation and the like, and the enteromorpha protein source antioxidant peptide has stable Keap1 binding capacity and remarkable antioxidant activity, and can be used for preparing a Keap1-targeted antioxidant peptide. The method can be widely applied to research and development of functional foods, medicines, skin care products, health products and the like, and has important economic values and good application prospects.

The invention belongs to the technical field of active peptide extraction and identification, and particularly relates to a cattle lung zymolyte, an antioxidant active peptide, a preparation method and application. According to the cattle lung zymolyte, the cattle lung is used as an extraction raw material, natural active peptide is extracted in an enzymolysis mode, the enzymolysis extract is collected and subjected to mass spectrum identification, bioinformatics analysis and experimental characterization are combined, 12 new antioxidant peptide sequences are obtained, the antioxidant effect of the antioxidant peptide sequences is verified, and the cattle lung zymolyte can be applied to natural antioxidant products.

The invention belongs to the technical field of biology, and particularly relates to a novel antioxidant peptide and application thereof, and the amino acid sequence of the novel antioxidant peptide is shown as SEQ ID NO.1. The antioxidant peptide is high in biological activity, has relatively high scavenging capacity on DPPH. Free radicals and ABTS. Free radicals, has relatively high reducing capacity on Fe < 3 + >, and has wide application in the aspects of preparing antioxidant medicines, foods or cosmetics and the like. Meanwhile, the antioxidant peptide is short in sequence, easy to synthesize and suitable for large-scale production and application.

This invention belongs to the field of food processing technology, specifically providing a method for preparing antioxidant zinc chelate peptides, comprising the following steps: inoculating a protein solution with a compound microbial agent for fermentation; the compound microbial agent includes *Lactobacillus plantarum* and *Saccharomyces cerevisiae*; after enzymatic hydrolysis and debittering of the fermentation broth, the antioxidant peptides are separated and dried to obtain the antioxidant peptides; the antioxidant peptides are dissolved in water to prepare a peptide solution, and a zinc source is added to the peptide solution to carry out a chelation reaction; the supernatant is collected, and the precipitate is collected by alcohol precipitation to obtain the antioxidant zinc chelate peptides. This method uses low molecular weight peptides prepared by dual-microbial synergistic multi-enzyme hydrolysis as chelating ligands, which chelate with zinc ions under mild conditions to maximize the preservation of biological activity, achieving a dual enhancement of chelation capacity and antioxidant activity, and achieving a unity of high chelation rate, high antioxidant activity, high stability and good flavor. The process is green, safe, and leaves no organic solvent residue, making it suitable for large-scale industrial production.