104 results about "Antioxidative peptide" patented technology

The invention relates to the technical field of biology, in particular to multifunctional belly-expanding hippocampal peptide as well as a preparation method and application thereof. Comprising a polypeptide with an amino acid sequence as shown in SEQ ID NO.1. The hippocampus antioxidative peptide is prepared by adopting an ultrahigh pressure pretreatment and enzymolysis technology, the adopted extraction process is simple in route, and the time and the production cost are effectively saved; an animal model is utilized to prove the anti-oxidation effect and the effect of improving male reproductive disorder and testis injury of the multifunctional belly-expanding hippocampal peptide; and on the basis, a novel peptide fragment with high activity, no toxicity and no sensitization is obtained.

The invention relates to the technical field of biology, in particular to a swelling hippocampal peptide as well as a preparation method and application thereof. Comprising a polypeptide with an amino acid sequence as shown in SEQ ID NO.1. The hippocampus antioxidative peptide is prepared by adopting an ultrahigh pressure pretreatment and enzymolysis technology, the adopted extraction process is simple in route, and the time and the production cost are effectively saved; an animal model is utilized to prove the antioxidation effect and the effect of improving male reproductive disorder and testis injury of the hippocampal swelling peptide; and on the basis, a novel peptide fragment with high activity, no toxicity and no sensitization is obtained.

The invention relates to the technical field of biology, in particular to a swelling hippocampus antioxidative peptide as well as a preparation method and application thereof. Comprising a polypeptide with an amino acid sequence as shown in SEQ ID NO.1. The hippocampus antioxidative peptide is prepared by adopting an ultrahigh pressure pretreatment and enzymolysis technology, the adopted extraction process is simple in route, and the time and the production cost are effectively saved; an animal model is used for proving the antioxidation effect of the hippocampus erectus antioxidative peptide and the effect of improving male reproductive disorder and testis injury; and on the basis, a novel peptide fragment with high activity, no toxicity and no sensitization is obtained.

The invention belongs to the technical field of food processing, and particularly relates to a method for synergistically extracting dogwood seed oil and antioxidant peptide by coupling supercritical CO2 extraction with deep-eutectic solvent DES. The method comprises the following steps: S1, performing microwave treatment on dogwood seeds, crushing and sieving to obtain dogwood seed powder; s2, the dogwood seed powder is subjected to supercritical CO2 extraction, a eutectic solvent serves as an entrainer, and residues A and dogwood seed oil are obtained after extraction is completed; s3, mixing the residue A with a deep eutectic solvent, and carrying out secondary extraction to obtain a residue B and a filtrate; s4, adding water into the filtrate, uniformly mixing, centrifuging, respectively collecting a water phase and an oil phase, carrying out reduced pressure distillation on the oil phase, and combining with the dogwood seed oil obtained in the step S2; and S5, adding the residue B obtained in the step S3 into water, and adding mixed enzyme to carry out enzymolysis reaction, so as to obtain the dogwood seed antioxidant peptide. According to the method, the dogwood seed oil and the antioxidant peptide can be extracted at the same time, the oil extraction rate of the dogwood seed oil reaches 90% or above, the yield of the antioxidant peptide is 78.7%, and the resource utilization rate is increased.

Provided in the present invention are a sturgeon extract rich in antioxidant peptides, and a preparation method therefor and the use thereof. The sturgeon extract of the present invention comprises an amino acid, polypeptide and protein from sturgeon meat and / or roe. The total amount of the amino acid, polypeptide and protein is 20% or more based on the dry matter content. On the basis that the total mass of the amino acid, polypeptide and protein is 100%, the molecular weight distribution of the amino acid, polypeptide and protein is as follows: the content of the component with a molecular weight less than 500 Da is 9%-60%; the content of the component with a molecular weight of 500 Da-1000 Da is 10%-30%; the content of the component with a molecular weight of 1000 Da-5000 Da is 5%-60%; and the content of the component with a molecular weight greater than 5000 Da is 5%-52%. The sturgeon extract of the present invention is rich in a variety of antioxidant peptides, has a relatively high antioxidant activity and also has the functions of protecting the liver and enhancing immunity.

The invention provides a sturgeon extract rich in antioxidant peptide as well as a preparation method and application of the sturgeon extract. The sturgeon extract provided by the invention comprises amino acids, polypeptides and proteins from sturgeon meat and / or roes; the total amount of amino acid, polypeptide and protein is more than 20% by dry matter content. Wherein the total mass of the amino acid, the polypeptide and the protein is 100%, and the molecular weight distribution of the amino acid, the polypeptide and the protein is as follows: the molecular weight is less than 500Da, and the content of the amino acid, the polypeptide and the protein is 9-60%; the molecular weight is 500 Da to 1000 Da, and the content is 10 to 30 percent; the molecular weight is 1000 Da to 5000 Da, and the content is 5 to 60 percent; the molecular weight is greater than 5000Da, and the content is 5-52%. The sturgeon extract disclosed by the invention is rich in various antioxidant peptides, and has the functions of protecting liver and enhancing immunity while having relatively high antioxidant activity.

The invention belongs to the technical field of biology, and particularly relates to a novel antioxidant peptide and application thereof, and the amino acid sequence of the novel antioxidant peptide is shown as SEQ ID NO.1. The antioxidant peptide is high in biological activity, has relatively high scavenging capacity on DPPH. Free radicals and ABTS. Free radicals, has relatively high reducing capacity on Fe < 3 + >, and has wide application in the aspects of preparing antioxidant medicines, foods or cosmetics and the like. Meanwhile, the antioxidant peptide is short in sequence, easy to synthesize and suitable for large-scale production and application.

This invention belongs to the field of food processing technology, specifically providing a method for preparing antioxidant zinc chelate peptides, comprising the following steps: inoculating a protein solution with a compound microbial agent for fermentation; the compound microbial agent includes *Lactobacillus plantarum* and *Saccharomyces cerevisiae*; after enzymatic hydrolysis and debittering of the fermentation broth, the antioxidant peptides are separated and dried to obtain the antioxidant peptides; the antioxidant peptides are dissolved in water to prepare a peptide solution, and a zinc source is added to the peptide solution to carry out a chelation reaction; the supernatant is collected, and the precipitate is collected by alcohol precipitation to obtain the antioxidant zinc chelate peptides. This method uses low molecular weight peptides prepared by dual-microbial synergistic multi-enzyme hydrolysis as chelating ligands, which chelate with zinc ions under mild conditions to maximize the preservation of biological activity, achieving a dual enhancement of chelation capacity and antioxidant activity, and achieving a unity of high chelation rate, high antioxidant activity, high stability and good flavor. The process is green, safe, and leaves no organic solvent residue, making it suitable for large-scale industrial production.

The invention discloses a calculation design method and system of antioxidant peptide, and belongs to the technical field of bioinformatics and polypeptide design. The method comprises the following steps: constructing an antioxidant peptide activity prediction model and an antioxidant peptide sequence generation model based on an adapted language model; and guiding a sequence generation model to generate a candidate antioxidant peptide sequence through a target-oriented optimization process including reinforcement learning by utilizing the output of the activity prediction model. By constructing the prediction and design integrated calculation framework, the novel antioxidant peptide can be efficiently and reliably found and designed, and the method can be applied to the technical fields of food preservation, health care product development and medical biology.

This invention relates to the field of bioactive peptide preparation technology, and discloses a method for preparing and applying a bifunctional antioxidant and anti-inflammatory peptide based on porcine skin collagen. The bifunctional peptide has the amino acid sequence GPSGPPGEKGP. Through peptidomics and computer simulation screening, this invention identified five novel antioxidant peptides with good solubility, non-sensitizing properties, and non-toxicity. Using an AFB1-induced HuH7 cell oxidative damage model, in in vitro cell experiments, GP-11 was shown to significantly reduce ALT, AST activity, and ROS levels, enhance the activity of SOD, CAT, and GSH-Px antioxidant enzymes, and activate the Keap1-Nrf2 pathway. It also reduced the levels of inflammatory factors TNF-α and IL-6 by downregulating the classical cGAS-STING inflammatory pathway. GP-11 exhibits both significant antioxidant effects and the ability to reduce the levels of inflammatory factors, demonstrating a dual function of antioxidation and anti-inflammation.

The invention relates to hair-blacking essence and a preparation method thereof. Screening a symbiotic bacterium metabolism extract with small molecule peptide metabolism regulation capability; by combining low-concentration azelaic acid and zinc lactate components, scalp grease oxidation and abnormal proliferation of propionibacterium are inhibited; the expression of IL-10 anti-inflammatory factors is induced, the expression of TNF-alpha and IL-6 is reduced, and the hair follicle microinflammation state is reduced; a Wnt / beta-catenin pathway activator is used for inducing the melanin stem cells to be in a static state; the FGF-2 and the EGF are combined in a micro-dose manner to promote the amplification into precursor cells; mITF regulation peptides and a small amount of trace copper ions are supplemented to induce directional differentiation into mature melanocytes; a microcapsule carrier system with a three-layer structure; the penetration efficiency is improved; antioxidative peptide rich in reduced glutathione, NAD and a promoting factor are used, so that melanocytes are protected from oxidative damage; a coenzyme Q10 type mitochondrial homeostasis regulator is introduced, so that the energy metabolism is improved, the cell aging is delayed, the targeting property is strong, and the effect is accurate.

The invention discloses a molecular docking screening method and application of hermetia illucens protein antioxidant peptides, and relates to the technical field of biologication.The method comprises the following steps that S1, hermetia illucens protein is prepared through enzymolysis, wherein hermetia illucens defatted protein powder serves as a raw material, enzymolysis is conducted through pepsin under the enzymolysis conditions that the pH is 2.0, the temperature is 40 DEG C, the enzyme adding amount is 3% of the protein mass, the enzymolysis time is 3 h, and enzymolysis liquid is obtained; after the reaction is finished, inactivating, centrifuging, collecting supernatant, and freeze-drying to obtain hermetia illucens protein hydrolysate; according to the molecular docking screening method and application of the hermetia illucens protein antioxidant peptide, the molecular docking technology is specifically applied to efficient screening of the hermetia illucens-sourced antioxidant peptide, KEAP1 is taken as a key target, high-activity candidate peptide is rapidly and accurately positioned from massive enzymolysis peptide fragments, and the screening efficiency of the hermetia illucens-sourced antioxidant peptide is improved. The defects that a traditional blind screening method is large in workload, long in period and high in cost are overcome, and the screening efficiency and the success rate are remarkably improved.

The invention discloses an antioxidant peptide derived from bullfrog mucus and application of the antioxidant peptide. The amino acid sequence of the antioxidant peptide is KFLNVVSGLLTSKYR, and the molecular weight of the antioxidant peptide is 1724 g / mol. Polypeptides are identified from bullfrog mucus through a high performance liquid chromatography technology for the first time, an unreported antioxidant peptide with antioxidant activity is screened out, the antioxidant peptide is synthesized through a solid phase method, and the antioxidant peptide derived from bullfrog mucus has the advantages of being safe, non-toxic and good in water solubility and stability and has good application prospects. After the vitamin B3 is combined with the vitamin B3, the oxidation resistance is remarkably improved, and the vitamin B3 can be widely applied to preparation of foods, cosmetics, medicines or health-care products and has important economic value and social significance.

The invention discloses hydrogel loaded with repair-promoting and antioxidant peptides as well as a preparation method and application of the hydrogel, and belongs to the technical field of preparation of medical materials. According to the preparation method, firstly, polypeptide with the amino acid sequence of CEYWVKWCGEKGPAGERG is synthesized, and the polypeptide has antioxidant activity and cell proliferation promoting activity and has good structural stability and enzymatic degradation resistance; and loading the polypeptide into the hyaluronic acid hydrogel to obtain the hyaluronic acid hydrogel. The prepared hydrogel loaded with the repair-promoting and antioxidant peptide has excellent injectable performance and cornea spreadability, can enhance the retention of the polypeptide on the ocular surface, effectively solves the technical problem of high administration frequency of the existing preparation, has good cell and tissue compatibility, and has no adverse reaction in vivo.

The present application relates to the technical field of biology, and particularly relates to an antioxidant peptide and ACE inhibitory peptide from tuna, and a preparation method and application thereof. The amino acid sequence of the polypeptide provided by the present application is shown as SEQ ID NO. 1-6. The present application takes tuna skin waste as raw material, obtains tuna skin collagen through enzymolysis, then performs liquid chromatography-tandem mass spectrometry identification and molecular docking screening on the tuna skin collagen enzymolysis product, and obtains polypeptides with antioxidant activity and polypeptides effectively inhibiting ACE activity. The method simplifies the protein extraction step. Meanwhile, the method can improve the deep processing level of the tuna industry, achieve high-value utilization, and reduce the environmental pressure on processing waste.

The application discloses wrinkle abalone visceral-derived antioxidant peptides and ACE inhibiting peptides, and a preparation method and application thereof. 50 With DPPH free radical scavenging activity and ACE inhibiting activity as the guidance, the enzymatic hydrolysate is separated and purified through a series of methods such as ultrafiltration, dextran gel chromatography and high performance liquid chromatography, and is combined with bioinformatics screening to obtain one DPPH free radical scavenging activity peptide and three ACE inhibiting peptides, the amino acid sequences of which are shown as SEQ ID NO. 1-4, and the IC 50 values are 3.05 mg / mL, 77.43 μM, 143.93 μM and 124.10 μM respectively, and the four polypeptides have great application value. The application realizes high-value utilization of abalone viscera, and provides a reliable basis for application of abalone by-products.

The application discloses an eggshell membrane peptide with antioxidant and improving exercise capacity of aging organism and application thereof, and belongs to the technical field of biology. The amino acid sequence of the eggshell membrane peptide is shown as SEQ ID NO. 1, and is specifically EQPWFPAG. A novel antioxidant peptide EQPWFPAG is identified from chicken eggshell membrane hydrolysate, and the eggshell membrane peptide can improve the exercise capacity and antioxidant stress capacity of an aging organism through verification. The eggshell membrane peptide provided by the application can be used for preparing an antioxidant, and can also be used for preparing functional food, health products and pharmaceutical compositions for improving the exercise function of an aging organism, and has great application value.

This invention provides an application of tartary buckwheat antioxidant peptide as a PPARα agonist. The tartary buckwheat antioxidant peptide (SEQ ID NO:1) is used to prepare a PPARα agonist, and this tartary buckwheat antioxidant peptide is used to prepare a drug for improving metabolic dysfunction-related fatty liver disease (MASLD). The tartary buckwheat antioxidant peptide of this invention provides a new drug candidate for improving MASLD and has broad clinical application prospects.

The invention relates to the technical field of biological medicine, and discloses a yak milk casein antioxidant peptide as well as a preparation method and application thereof, and the preparation method comprises the following steps: step 1, dissolving yak milk casein in alkali liquor to obtain a casein solution; step 2, carrying out enzymolysis on the casein solution by using protease to obtain enzymatic hydrolysate; step 3, inactivating and centrifuging the enzymatic hydrolysate, taking supernate, and freeze-drying to obtain a crude polypeptide product; and step 4, purifying the polypeptide crude product through anion exchange column chromatography and gel filtration chromatography in sequence to obtain the antioxidant peptide component. The preparation method is efficient and clear in target, yak milk casein is subjected to enzymolysis through a single enzyme / complex enzyme combined strategy, a two-step purification process of DEAE-52 cellulose column chromatography and SephadexG-50 gel chromatography is combined, the high-activity polypeptide component is efficiently and repeatedly prepared, and the defect that traditional enzymolysis product components are complex is overcome.

The invention relates to pressed salted duck antioxidant peptide and a preparation method thereof. The method comprises the following steps: carrying out synergistic inoculation and fermentation treatment on lactobacillus curvatus and staphylococcus mimicus, and carrying out pickling, air drying and peptide extraction processes to obtain the small molecular peptide with the molecular weight of less than 10 kDa. Through nano-LC-MS / MS (Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) detection, computer virtual screening and molecular docking, three peptide sequences, namely GIRLGLDPKL, PAPAAKAGASTGRIV and PEKPPTIDWA, with remarkable antioxidant activity are determined. According to the method, protein degradation can be effectively promoted, the yield of the antioxidant peptide is increased, and the obtained peptide has good stability and safety and is suitable for development of functional food and health care products.

This invention discloses an antioxidant peptide derived from zebrafish and its applications. The amino acid sequence of the antioxidant peptide is SATVAIAIREILKK, and its molecular weight is 1499.67 g / mol. This application is the first to identify peptides from zebrafish using high-performance liquid chromatography (HPLC) and screen for a previously unreported antioxidant peptide with antioxidant activity. This zebrafish-derived antioxidant peptide is synthesized via a solid-phase method. It is safe, non-toxic, has good water solubility and stability. Its antioxidant activity is significantly enhanced when combined with vitamin B7. It can be widely used in the preparation of food, cosmetics, pharmaceuticals, or health products, and has significant economic and social value.

The application discloses active peptides with antioxidant and alcoholic liver injury protection efficacy and application thereof. The application takes walnut protein enzymatic hydrolysate as raw material, and two novel high-antioxidant activity peptide segments HGEPGQQQR and VAPFPEVFGK are screened. The two active peptides show good antioxidant activity at a cell level, and the EC 50 values thereof are 0.0120 mg / mL and 0.0068 mg / mL respectively, which are better than 0.0122 mg / mL of a positive control glutathione. In an ethanol injury model, the two antioxidant peptides can increase the survival rate of cells treated by ethanol from 47.35% to 62.69% and 57.05% respectively. The results show that the walnut protein-derived antioxidant peptides HGEPGQQQR and VAPFPEVFGK can be developed and used as a potential antioxidant and liver protection active ingredient.

The invention belongs to the technical field of bioactive peptide preparation, and discloses a preparation method of a tylorrhynchus antioxidant peptide, which comprises the following steps: mixing tylorrhynchus homogenate with enzyme, and carrying out enzymolysis to obtain the tylorrhynchus antioxidant peptide, the enzyme comprises flavourzyme and pancreatin. The invention provides the preparation method of the tylorrhynchus antioxidant peptide which is high in antioxidant activity and suitable for industrial mass production, the protein hydrolysis degree is improved, and the oxidation resistance of the tylorrhynchus antioxidant peptide is improved. The method can effectively solve the problems of low efficiency, high cost and inapplicability to large-scale extraction and preparation of the tylorrhynchus antioxidative peptide in the existing polypeptide extraction process.

The invention belongs to the technical field of biological peptides and application, and particularly relates to an antioxidant peptide with a Keap1 inhibiting effect and application of the antioxidant peptide. The antioxidant peptide provided by the invention comprises antioxidant pentapeptide, antioxidant tripeptide and the like which all show good in-vitro antioxidant activity, the scavenging rate of ABTS free radicals reaches 0.69-1.11 [mu] mol TE / [mu] mol, the scavenging rate of DPPH free radicals reaches 30.11%-34.94%, in addition, the antioxidant peptide can activate the expression of Nrf2 and downstream genes HO-1 and NQO1 thereof by inhibiting the expression level of Keap1 protein, so that the antioxidant activity of the antioxidant peptide is improved. Therefore, the compound has a wide application prospect in the fields of cosmetics, medicines, foods and the like.

The application discloses an antioxidant peptide derived from bullfrog mucus and application thereof, and the amino acid sequence of the antioxidant peptide is KFLNVVSGLLTSKYR, and the molecular weight is 1724 g / mol. The polypeptide is identified from the bullfrog mucus by high performance liquid chromatography for the first time, and an unreported antioxidant peptide with antioxidant activity is screened out. The antioxidant peptide derived from the bullfrog mucus is synthesized by a solid phase method, and has the characteristics of safety, non-toxicity, good water solubility and stability. After being combined with vitamin B3, the antioxidant property of the antioxidant peptide is significantly improved, and the antioxidant peptide can be widely applied to preparation of cosmetics, medicines or health products, and has important economic value and social significance.

The invention provides a compound peptide beverage capable of dispelling effects of alcohol and protecting liver as well as a preparation method and application thereof. The compound peptide beverage is prepared from hovenia dulcis thunb oligopeptide, walnut peptide, antioxidant peptide, energy release auxiliary peptide, ethanol dehydrogenase activating peptide, silibinin-serine chelate, an electrolyte poising agent, alginic acid oligosaccharide and purified water. Wherein the antioxidant peptide is prepared from cordyceps militaris, sea-buckthorn and sunflower disc through mixed enzymolysis, the energy release auxiliary peptide is prepared from carnosine and arginine succinic acid peptide through microbial fermentation, and the ethanol dehydrogenase activating peptide is prepared from marine shellfish collagen through fermentation. The composite peptide beverage shows excellent functions of dispelling the effects of alcohol and protecting the liver, and can be used for developing medicinal products or functional foods for dispelling the effects of alcohol and protecting the liver. Through precise compatibility and an innovative process technology, the product constructs a unique solution for dispelling the effects of alcohol and protecting the liver, and has outstanding performance in the aspects of improving the alcohol metabolism efficiency and relieving liver injury.

The invention discloses an apostichopus japonicus extract antioxidant peptide as well as a preparation method and application thereof, and relates to the technical field of biological medicines. The amino acid sequence of the apostichopus japonicus essence antioxidant peptide is shown as SEQ ID NO. 1. The apostichopus japonicus essence antioxidant peptide has the advantages that the apostichopus japonicus essence antioxidant peptide is prepared; the antioxidant peptide with the amino acid sequence of LPPF is screened from the apostichopus japonicus essence, and the peptide is proved to have excellent antioxidant activity and high safety, and has a better application prospect in treatment of oxidative stress injury diseases.

The invention discloses a polypeptide function prediction method based on machine learning. The method comprises the following steps: collecting data information of wound healing peptides, hypoglycemic peptides, antioxidant peptides and anticancer peptides, analyzing different characterization data of polypeptide sequences by utilizing biological new informatics software, constructing a polypeptide characterization database, and dividing training, verification and test data sets; important features for determining polypeptide function classification are obtained through data preprocessing and feature screening; based on the important feature data, performing model construction on the data by using different machine learning algorithms to obtain an algorithm with the best performance for model optimization; and further performing performance verification on the optimization model on the verification set and the test set. The invention constructs a polypeptide function prediction tool based on a C50 algorithm, and the function of the position function polypeptide can be efficiently and accurately evaluated.

Provided are gamma glutamylcysteine synthetase variants and microbial cells comprising such variants and / or expressing heterologous gamma-glutamylcysteine synthetase-glutathione synthetases, for producing increased amounts of the antioxidant peptide glutathione (GSH). Methods and compositions provided herein may be useful in baking and winemaking applications and in production of nutraceuticals, pharmaceuticals, cosmetics, plant protectants and / or animal feeds.

The present invention discloses an antioxidant and degradable composite food packaging film. The composite food packaging film uses sodium carboxymethyl cellulose and cassava starch as film-forming matrices and uses defatted Antarctic krill meal antioxidant peptides as an antioxidant. By adding defatted Antarctic krill meal antioxidant peptides to a bio-based film, the present invention reduces the water contact angle and crystallinity, increases the water vapor permeability, enhances the antioxidant capacity, thermal stability and mechanical properties, changes the color of the composite film, and improves the barrier property to ultraviolet light.