302 results about "Antioxidative peptide" patented technology

The invention discloses a minced tuna protein antioxidative peptide and its preparation method and use. The minced tuna protein antioxidative peptide has an amino acid sequence of Tyr-Glu-Asn-Gly-Gly. The preparation method comprises the following steps of adding a phosphate buffer solution into defatted minced tuna as a raw material, adjusting a pH value of the mixture to 5.0 to 7.0 to obtain a mixed solution, preheating the mixed solution with stirring, adding papain into the preheated mixed solution to obtain an enzymolysis product, carrying out enzyme deactivation of the enzymolysis product, and orderly carrying out desalination, ultrafiltration and chromatography to obtain the minced tuna protein antioxidative peptide. The preparation method has the advantages of scientific and reasonable processes and easy monitoring of enzymolysis. The minced tuna protein antioxidative peptide obtained by the preparation method has the advantages of safety, no toxic and side effect, strong antioxidant activity and easy digestive absorption, and can be used as a drug, a health-care food and a food additive.

The invention relates to a mussel meat protein antioxidative peptide and a preparation method thereof. The invention is characterized in that the antioxidative peptide is a pentapeptide compound with an amino acid sequence of YPPAK (Tyr-Pro-Pro-Ala-Lys), and according to ESI-MS detection, a molecular ion peak of m/z [M+H]<+> 575.26 is given out. The preparation method comprises the steps consisting of slurry homogenizing, degreasing, mixing, enzymatic hydrolysis, desalination, ultrafiltration, chromatography and the like. The prepared high-activity antioxidative peptide YPPAK (Tyr-Pro-Pro-Ala-Lys) has a good removal effect on DPPH free radicals, hydroxyl free radicals and superoxide anion free radicals; meanwhile, the YPPAK (Tyr-Pro-Pro-Ala-Lys) shows a good inhibitory effect on lipid peroxidation. The YPPAK (Tyr-Pro-Pro-Ala-Lys) has the advantages of safety, no toxic and side effects, good antioxidant activity, easy digestion and absorption, etc., and can be used as a medicine, a health food or foodstuff additive and the like.

The invention discloses a preparation method of an antioxidant peptide derived from mytilus coruscus, which comprises the following steps: 1) adding water into mytilus coruscus meat degreased dry powder serving as a raw material; 2) homogenizing materials obtained by the step 1) first, then preheating, adjusting the pH value to 7.0 to 9.0, and adding protease to start an enzymolysis reaction; and3) adjusting the pH value to 6.9 to 7.1 at the end of the enzymolysis reaction, inactivating enzyme, centrifuging, enriching active peptides by ultrafiltration and finally obtaining the antioxidant peptide by concentration and freeze drying. The invention also discloses the use of the antioxidant peptide obtained by the preparation method as an active functional ingredient. The antioxidant peptide obtained by the preparation method has high activity.

The invention relates to a method for preparing an antioxidant peptide through ultrasonic-microwave synergetic enzymatic hydrolysis. The method comprises the following steps: (1), preparing an enzymatic hydrolysis substrate; (2), adding an alkali protease for enzymatic hydrolysis; (3), carrying out ultrasonic-microwave assistant enzymatic hydrolysis; and (4), after enzyme inactivation, centrifuging and separating a reaction solution, taking supernatant, freezing and drying to obtain antioxidant peptide powder. The method has the advantages that the ultrasonic technology, the microwave technology and the enzymology biology technology are combined. Compared with the peptide prepared by the conventional method, no chemical reaction is carried out, the change rate of bioactive substances is reduced, the utilization rate of organic solvents is reduced, the enzymatic hydrolysis time is shortened after the ultrasonic-microwave assistant enzymatic hydrolysis is carried out, and a test proves that the antioxidant capacity of the salmon peptide is remarkably enhanced. The method is simple in process, can be used for industrial production and has higher economic and social benefits.

The invention relates to an antioxidant peptide from collagen, which has one of amino acid sequences: (1) Hyl-Cys; (2) Gln-Gly-Ala-Arg; (3) Leu-Gln-Gly-Met; and (4) Leu-Gln-Gly-Met-Hyp. The antioxidant peptide can be synthesized by a peptide synthesizer chemical method or can be synthesized by hydrolyzing the collagen and separating and purifying the obtained hydrolysate. The antioxidant peptide not only has strong antioxidant function, but also can replace BHT (Butylated Hydroxytoluene) and the like to be used as an addictive of food as well as can be used as an active functional component. The antioxidant peptide can be used for foods, heath-care products, cosmetics, even animal food and feed and the like and has extremely-wide application range and remarkable social benefits and economic benefits. In addition, the antioxidant peptide has 2-5 amino acids and small molecule weight and is more easily absorbed by alimentary tract.

The invention discloses a process technique for preparing wheat gluten powder peptide by a fermentation and enzymolysis method. A wheat gluten powder active peptide product with relatively high antioxidant activity is obtained by using a microbial fermentation and enzymolysis method, hoping for providing technique support for industrial production of preparing antioxidative peptide by using wheat gluten powder, thereby expanding the application range of the wheat gluten powder and improving the production additional value of the wheat gluten powder. The wheat gluten powder product obtained by adopting ultrasonic-assisted enzymolysis and an aerobic anaerobic two-step fermentation process is white in color and luster, powdery and free from impurities and bitter taste. The total protein content is greater than or equal to 97%, and the content of peptide with the molecular weight which is smaller than 1000 Dalton in total protein is greater than or equal to 98%. The reducing capacity of the wheat gluten powder peptide reaches 0.486 and the ABTS free radical scavenging capacity reaches 1.85mmol/L Trolox. The method disclosed by the invention is short in production period, low in cost and free from generation of any toxic and harmful substances. The obtained product is high in safety and good in functional activity and can be widely applied to the fields such foods, drugs, health products and biological synthesis.

The invention discloses a process method for quickly preparing a soybean antioxidant peptide, belonging to the technical fields of deep processing of agricultural and sideline products and comprehensive utilization of byproducts thereof, and relating to the process method for quickly preparing the soybean antioxidant peptide by using a microwave technology. According to the process method, soybean protein concentrate powder with protein content of 70 percent is used as a raw material, the material passes through a microwave extractor through processes of preparing the soybean protein concentrate powder, pretreating soybean protein concentrate powder emulsion, performing microwave auxiliary enzymatic treatment on the soybean protein concentrate powder emulsion, deactivating enzymatic hydrolysate, centrifuging, performing ultrafiltration treatment and performing vacuum freeze drying treatment by using the microwave auxiliary enzymatic hydrolysis technology; the enzymatic hydrolysis time is shortened to 35-38 minutes; and compared with the conventional enzymatic hydrolysis, the efficiency is increased by 3-4 times, and the ABTS (2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid)) free radical clear rate is over 72 percent and is equivalent to that of the conventional enzyme. According to the process method, a new idea is provided for technical research for quickly preparing the soybean antioxidant peptide, and a new approach is provided for development and application of soybean series products of high antioxidant activity.

The invention discloses an antioxidative peptide of a shark protein as well as a preparation method and use thereof. The amino acid sequence of the antioxidative peptide is Leu-Asp-Lys. The molecular weight at the molecular ion peak m/z detected by ESI-MS (Electrospray Ionization-Mass Spectrometry) is 375.30[M+H]<+>. The preparation method comprises the following steps: by taking meat of degreased shark as a raw material, adding a buffer liquid in a solid-to-liquid ratio of 1g:(15mL-20mL), and adjusting the pH to 5.0-7.0 by 0.05-0.15mol/L of HCl or 0.05-0.15mol/L of NaOH to obtain a mixed liquid; heating the mixed liquid to 50-60 DEG C, stirring and pre-heating for 5-10 minutes, and adding 0.8-1.2% of papain based on weight of degreased meat to obtain an enzymolysis product, wherein the enzymolysis temperature is 50-60 DEG C and the enzymolysis time is 1-3 hours; firstly carrying out enzyme deactivation on the enzymolysis product to obtain an enzymatic liquid, and then sequentially carrying out desalination, ultrafiltration and chromatography on the enzymatic liquid to obtain the antioxidative peptide. According to the invention, the preparation process is scientific and reasonable, the enzymolysis process is easy to monitor, and the prepared antioxidative peptide has the advantages of safety, no toxic or side effects, strong antioxidant activity, easiness in digestion and absorption and the like, and can be applied as medicines, health-care foods, food additives and the like.

The invention discloses an antioxidant peptide of hairtail fish protein as well as a preparation method and uses of the antioxidant peptide. In particular, hairtail fish is used as raw materials; an enzymatic hydrolysis solution is obtained through degreasing and enzymolysis of protamex and neutral protease; the enzymatic hydrolysis solution is separated and purified through ultra-filtration, purification of macroporous resins, cation exchange resin chromatography, gel column chromatography, and reversed-phase high-performance liquid chromatography so as to obtain the antioxidant peptide Asn-Trp-Asp-Met-Glu-Lys-Ile-Trp, and the ESI-MS determining molecular weight is 1121.25da. The high-activity antioxidant peptide disclosed by the invention has a good scavenging effect on DPPH free radicals, hydroxyl radicals and superoxide anion free radicals; besides, the antioxidant peptide shows a good effect of lipid peroxidation inhibition. The antioxidant peptide has the advantages of safe use, zero toxic or side effects, high oxidative activity and the like, is liable to digest and absorb, and can be used as medicines, health food or food additives, and the like.

The invention discloses an antioxidant peptide prepared from shark prolamin and a preparation method and application thereof. The amino acid sequence of the antioxidant peptide is Trp-Asp-Arg or/and Pro-Tyr-Phe-Asn-Lys; according to the ESI-MS detection, a molecular ion peak is m/z 476.40[M+H]<+> or/and m/z 668.60[M+H]<+>; when in preparation, the shark meat is used as a raw material, and the shark prolamin is extracted by use of ethanol; then, papain is used as an enzymolysis enzyme, and the antioxidant peptide is prepared by the enzymolysis technology in combination with macroporous resin desalination, ultrafiltration classification and chromatography refining. The preparation method disclosed by the invention is scientific and reasonable in process, the enzymolysis process is easy to monitor, and the prepared antioxidant peptide has relatively high activity; compared with a chemosynthetic antioxidant, the antioxidant peptide disclosed by the invention has the advantages of safety, no toxic or side effect, high antioxidant activity, easiness in digestion absorption and the like, and can be used as a medicine, a healthcare food, a food additive and the like.

The invention discloses a preparation method of an antioxidative peptide of a hairtail. The preparation method comprises the following steps of 1, adding water into hairtails, and mincing the hairtails into hairtail paste, 2, adding the hairtail paste into an enzymolysis tank, and adding endoprotease into the hairtail paste for hydrolysis, wherein the mass of the endoprotease is 0.2 to 0.9% of the mass of the hairtails, 3, carrying out centrifugation of a hydrolysate obtained by the step 2, and collecting a supernatant, 4, carrying out microfiltration of the supernatant, adding the micro-filtrated supernatant into the enzymolysis tank, and adding excision protease into the enzymolysis tank for hydrolysis, wherein the mass of the excision protease is 0.1 to 0.9% of the mass of the hairtails, 5, in the hydrolysis, pumping an enzymatic hydrolysate into an ultrafiltration membrane separator from the enzymolysis tank so that the enzymatic hydrolysate can be circulated at a flow of 0.1 to 0.5L/min, and 6, collecting an ultrafiltration membrane permeate, carrying out reduced pressure concentration until a volume of the ultrafiltration membrane permeate is 1/10 to 1/5 of the original volume, keeping a temperature of -18 to -20 DEG C for a night, and carrying out freeze drying at a temperature of -60 to -65 DEG C under the pressure of 0.005 to 0.0054 mbar to obtain the antioxidative peptide of hairtails. The antioxidative peptide of hairtails has strong antioxidation activity, a hydroxyl radical removal rate of 82.0%, a superoxide anion radical removal rate of 85.2% and a DPPH radical removal rate of 61.5%.

The present invention is antioxidant peptide mixture obtained through hydrolyzing collagen and its preparation process. The preparation process includes hydrolyzing collagen with protease to obtain coarse product of antioxidant peptide mixture; precipitating or filtering to obtain hydrolysate with fat, macro molecular protein, polypeptide and impurity eliminated; and final separating purification and concentration to obtain the antioxidant peptide mixture. The present invention has wide material source and low cost, and the product has powerful antioxidant effect, and may be used as food additive as well as in cosmetics, health product, feed, etc.

The invention discloses a method for preparing strong antioxidative peptide by using peanut meal. The method comprises the following steps of: (1) uniformly mixing the peanut meal and an acidic buffer, carrying out microwave heating, and placing in a powdering machine, thus obtaining powder peanut meal; (2) adding water, protease and cellulase, then adding a surface active agent, hydrolyzing at 45-60 DEG C, and carrying out centrifugal separation to obtain liquid supernatant which is peanut meal enzymatic hydrolysate; and (3) adding an ethanol solution to the peanut meal enzymatic hydrolysate, carrying out ultra-filtration, collecting trapped fluid, and carrying out spray drying to obtain the strong antioxidative peptide. According to the method, the peanut meal is pretreated by adopting microwave heating and the acidic buffer, so that disulfide bonds in peanut meal proteins are converted into sulfydryl bonds, more enzymolysis sites are exposed, and the enzymatic hydrolysis is facilitated. The surface active agent is used for cooperating with the enzymatic hydrolysis, changing the characteristics of an enzymolysis substrate, enlarging a surface on which celluloses can be adsorbed, and sequentially simulating reaction sites to be adsorbed on enzymes; and meanwhile, the surface active agent has stablizing effect on the enzymes, effectively stops the denaturation of enzymes in a hydrolytic process and is beneficial to the improvement of hydrolysis efficiency of the proteases.

The invention provides an antioxidative peptide and a preparation method thereof. The method is used for obtaining the specific antioxidative peptide from chlorella protein as the raw material by virtue of enzymolysis of an acid proteinase, separation and purification; the amino acid complete sequence of the specific antioxidative peptide is aetiglap. The shortcomings of a natural antioxidant are overcome and the worry of the public on an artificially synthetic antioxidant is eliminated, and a foundation is laid for developing the antioxidative peptide based on food sources and exploring the wide application of the antioxidative peptide in foods and medicines.

The invention discloses a preparation method of pig serum protein antioxidant peptide by adopting microbial fermentation and a product thereof. The preparation method comprises steps of: (1) carrying out micro-filtering on pig serum protein; (2) inoculating the filtered pig serum protein with bacillus subtilis bacterial suspension and carrying out fermentation; and (3) decentralizing fermentation liquor, collecting liquid supernatant, and carrying out concentrating, freezing and drying to obtain the finished product. The pig serum protein antioxidant peptide prepared by the method has higher antioxidation activity, capability of eliminating free radical and better antioxidation effect both in vitro and in vivo. The preparation method is simple for operation, and is safe and reliable compared with the method of synthesizing antioxidant; and the preparation method is applicable to industrial production and can greatly enhance the utilization rate of pig serum protein resource and raise the comprehensive utilization level of pig blood so as to effectively increase additional value of pig blood resource.

The invention discloses an antioxidation active synthetic peptide and a purpose thereof. a polypeptide provided by the invention is any one of the following polypeptides 1) to 3): 1) a polypeptide shown by a sequence I in a sequence table; 2) a polypeptide shown by a sequence 2 in the sequence table; and 3) a polypeptide shown by a sequence 3 in the sequence table. According to the invention, it is proved by experiments that three polypeptides with antioxidation activity are synthesized; as the antioxidation peptide has the advantages of small molecular weight, high activity, easiness in artificial synthesis, low cost and so on, the antioxidation peptide can be widely applied to large-scale production in industries, such as foods, cosmetics, medicines and the like; and the antioxidation active synthetic peptide disclosed by the invention can be specifically used as an antioxidant in aspects, such as cosmetics, food healthcare products, animal feed additives, medicines and the like.

The invention relates to a pseudosciaena crocea flesh antioxidative peptide and use thereof, and the pseudosciaena crocea flesh antioxidative peptide is characterized in that: the amino acid sequence is Tyr-Leu-Met-Ser-Arg, and the molecular weight is 651.77Da ((M + H) + 652.45Da) given by ESI (electronic spray ion)-MS (mass spectrum) detection. The preparation method of the antioxidative peptide includes homogenizing, enzymolysis, ultrafiltration and chromatography and other steps. The prepared antioxidative peptide Tyr-Leu-Met-Ser-Arg has good scavenging effect on DPPH (1,1-diphenyl-2-picryl-hydrazyl) free radical, hydroxyl radical, ABTS radical and superoxide anion free radical; at the same time, the Tyr-Leu-Met-Ser-Arg also shows good lipid peroxidation inhibition effect. The Tyr-Leu-Met-Ser-Arg has significant antioxidant activity, and can be used as medicines, health food or food additives.

This invention relates to a series of antioxygen peptide stem from collagen protein. It possess one of as follows amino acid sequence: ( 1) Hyl - Cys; ( 2) Gln - Gly - Ala - Arg; ( 3) Leu - Gln - Gly - Met; ( 4)) Leu - Gln - Gly - Met - Hyp. This antioxygen peptide could synthesize through polypeptide instrument chemical process, as well as through hydrolization of collagen protein, and then obtain by separating and purifying acquired hydrolysate. The antioxygenation of this antioxygen peptide is very strong, not only could substitute BHT to use as edible additive, furthermore could as activating functional ingredient. This product could be used in foodstuff, health products, makeup, even animal foodstuff and feedstuff, has abroad field of application and notable social benefit and economic benefit. The antioxygen peptide only has two to five amino acid, molecular weight smallness, more easily directly assimilate by alimentary tract.

The invention discloses a method for preparing an antioxidative peptide from skipjack processing by-products. The method comprises the following steps of mixing head sections and tail sections as by-products rich in proteins and a stewing liquor, crushing the mixture, carrying out synchronous three-phase separation to obtain a protein source which can be subjected to direct enzymolysis, adding a flavourzyme into the protein source, carrying out enzymolysis at a pH value of 6.5 at a temperature of 50 DEG C for 4h, purifying the enzymatic hydrolysate by ultrafiltration, activated carbon adsorption and desalination, and carrying out spray drying to obtain peptide powder. Various antioxidant activities of the peptide powder are detected. The peptide powder has good DPPH free radical, superoxide anion free radical and hydroxyl radical removal rates, has IC50 of 3.55mg/ml, 1.39mg/ml and 0.61mg/ml and is an excellent anti-oxidant. The peptide powder is flavescent, has no bitter taste, has a light fishiness taste, has molecular weight (greater than 90.1%) less than or equal to 10000Da, has polypeptide total protein content of 88.6% by dry weight and has ash content of 7.2%.

The invention relates to rana pleuraden antioxidant peptide and genes and an application thereof, belonging to the technical field of biomedical science. The rana pleuraden antioxidant peptide is thesingle chain polypeptide through genetic encoding of Chinese amphibians rana pleuraden, wherein, the molecular weight is 1653.88 daltons, the isoelectric point is 8.22, and the complete sequence of the rana pleuraden antioxidant peptide is NH2-GIRPTYNRQCEIGF-COOH; the gene for encoding is composed of 316 nucleotides, wherein, the encoding mature part is the 130-171th nucleotide. The synthetic ranapleuraden antioxidant peptide features strong antioxidant activity, can be applicable to the preparation of medicines of skin antioxidant protection and free radicals in vivo elimination, and has theadvantages of simple sequence and convenient synthesis.

The invention discloses duck gizzard duck's gizzard antioxidative peptide and application thereof. The amino acid sequence of the duck gizzard duck's gizzard antioxidative peptide is as shown in Asn-Ly-Phe-Ile-Leu-Lys (a component DKFILK), and the monoisotopic peak of a parent ion of the sequence is m/z 382.2376 Da. The preparation process comprises the following steps: degreasing duck gizzard, performing enzymolysis, performing ultrafiltration on an enzymolysis product, performing gel filtration chromatography, performing reverse high performance liquid chromatography purification, and performing mass spectrum together with amino acid analysis, thereby obtaining the antioxidative peptide. The duck gizzard duck's gizzard antioxidative peptide is wide and cheap in raw material source and scientific and reasonable in process; by virtue of an enzymolysis technique and together with purification through ultrafiltration grading and chromatographic analysis, the prepared antioxidative peptide has relatively high activity; compared with an antioxidant prepared through chemical synthesis, the antioxidative peptide disclosed by the invention has the advantages of security, no toxic or side effect, good antioxidation property, easiness in digestion and absorption, and the like, and can be used as medicines, functional food and the like.

The invention provides a method for synchronously extracting grease and antioxidative peptide from soybeans and belongs to the food processing technology. The method comprises steps as follows: (1), the soybeans are smashed and sieved, water is added for soaking, and a semi-solid mixed solution is formed; (2), the semi-solid mixed solution is pretreated with a high-voltage pulse electric field; (3), three-enzyme compounds of Protex-7L neutral protease, alkaline protease and bromelain are added to the mixed solution after being subjected to pulse electric field treatment for enzymolysis; (4), the mixed solution after enzymolysis is cooled to 4 DEG C, and soybean grease and hydrolysate are obtained through centrifugation and separation; (5), the hydrolysate is taken and subjected to closure separation by a 10kDa ultrafiltration membrane (a PP-100 hollow-fiber module), and a soybean peptide solution with molecular weight of 10 kDa or lower is obtained; (6), the soybean peptide solution is subjected to vacuum freeze drying, and the antioxidative soybean peptide with high added value can be directly obtained.

The invention relates to a method for preparing navodon septentrionalis skin anti-oxidative peptide liquid through in vitro simulation of gastrointestinal digestion and belongs to the fields of food processing technology and protein utilization technology. The adopted preparation process comprises the following steps: preprocessing raw material navodon septentrionalis skin by shearing, drying, grinding, etc.; through an in vitro gastrointestinal digestion simulation technology, simulating a gastrointestinal digestion system through dual enzyme constructed from pepsase and trypsin; inactivating enzyme through a boiling water bath, regulating pH value and integrating twice centrifugal means to prepare the fish skin anti-oxidative peptide liquid. The preparation method disclosed by the invention, through a dual-enzyme digestion reaction system, greatly improves hydrolysis efficiency of fish skin protein and realizes full utilization of raw material; the process of in vitro simulation of gastrointestinal digestion is implemented in 37 DEG C water bath; an enzymolysis condition is mild and similar to a human body, and a digestion process of a human body can be better reflected.

The invention relates to a natural biological preserving agent for aquatic products, which is prepared from the following ingredients in parts by weight: 16 to 24 parts of chondroitin sulfate oligosaccharide, 35 to 40 parts of antioxidative peptide, 41 to 45 parts of curcuma extract and 300 to 450 parts of sterilized water. The invention further relates to a preparation method of the biological preserving agent and application to preservation of the aquatic products. The biological preserving agent disclosed by the invention is non-toxic, has no residues, is low in cost, can effectively avoid oxidative damage to fat, proteins and the like of the aquatic products, and keeps the bright color of the aquatic products; meanwhile, the broad-spectrum antibacterial activity of the curcuma extract is sufficiently utilized to prevent breeding and reproduction of various common bacteria of the aquatic products in the preserving process of the aquatic products, so that the natural biological preserving agent takes an effective preserving effect on the aquatic products and a preservation period of the aquatic products is obviously prolonged. Moreover, in the preparing process of the preserving agent, both the chondroitin sulfate oligosaccharide and the antioxidative peptide use aquatic product processing byproducts as raw materials, so that production cost can be reduced, the output value of aquatic product processing is improved, the environment is improved, and the natural biological preserving agent has obvious social effects.

The invention relates to a polymicrobic fermentation agent and a polymicrobic fermentation milk product for preventing atherosclerosis and a production method thereof. The polymicrobic fermentation milk product is prepared by the following steps of : step 1, heating milk or skimmed milk; carrying out pasteurization, cooling to 40-45 DEG C, adding the polymicrobic fermentation agent, and fermenting; step 2, after fermentation, maturing under the constant temperature; step 3, uniformly mixing, extracting fat, and boiling the rest of the mixture for 1-2 hours under the temperature of 50-60 DEG C; and step 4, separating whey: carrying out sterilization and filter cloth filtration under the temperature of 18-23 DEG C so as to respectively obtain cheese and the whey, wherein the whey is the polymicrobic fermentation milk product. The invention is simple to operate and has a simple production method, the obtained polymicrobic fermentation milk product has rich nutrients, and contains probiotics, ACE inhibitory peptide, antioxidant peptide, and the like, has the functions of preventing the atherosclerosis, adjusting blood fat, preventing inflammatory, antioxidation, and the like, can adjust the intestinal tract bacteria colony to be balanced, and can delay senility.

The invention belongs to the technical field of the deep-processing of egg products and the comprehensive utilization of side products, and discloses an egg white source antioxidant peptide powder and a preparation method thereof. The egg white source antioxidant peptide powder can be prepared by taking hydrolyzed egg white protein as a raw material and DPPH (1,1-diphenyl-2-picryhydrazyl) free radical scavenging rate and reducing power as antioxidant activity evaluation indexes through using an ultrafiltration device, a vacuum rotating concentration instrument, a column chromatography cabinet system and a vacuum freeze-drying technology. In the egg white source antioxidant peptide powder, the molecular weight is 300-600Da, the protein content is 94.28%, the DPPH scavenging rate is 90.2%, the reducing power is 1.8, and the moisture content is 1%-3%. According to the preparation method, the antioxidant activity of the egg white source antioxidant peptide can be improved, the DPPH scavenging rate is improved to 90.2% from 50%, the reducing power is improved to 1.8 from 1.46, and the research and development level of egg resources on comprehensively utilizing and developing functional products can be beneficially improved.