Method for preparing antioxidative peptide from skipjack processing by-products

A technology for antioxidant peptides and by-products, which is applied in the field of marine bioengineering enzymatic production technology, can solve the problems of the lack of research on antioxidant peptides, and achieve the effects of good antioxidant activity, strong feasibility and low price.
CN103540638AActive Publication Date: 2014-01-29FUJIAN PUTIAN SEA 100 FOOD
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
FUJIAN PUTIAN SEA 100 FOOD
Publication Date
2014-01-29

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Abstract

The invention discloses a method for preparing an antioxidative peptide from skipjack processing by-products. The method comprises the following steps of mixing head sections and tail sections as by-products rich in proteins and a stewing liquor, crushing the mixture, carrying out synchronous three-phase separation to obtain a protein source which can be subjected to direct enzymolysis, adding a flavourzyme into the protein source, carrying out enzymolysis at a pH value of 6.5 at a temperature of 50 DEG C for 4h, purifying the enzymatic hydrolysate by ultrafiltration, activated carbon adsorption and desalination, and carrying out spray drying to obtain peptide powder. Various antioxidant activities of the peptide powder are detected. The peptide powder has good DPPH free radical, superoxide anion free radical and hydroxyl radical removal rates, has IC50 of 3.55mg / ml, 1.39mg / ml and 0.61mg / ml and is an excellent anti-oxidant. The peptide powder is flavescent, has no bitter taste, has a light fishiness taste, has molecular weight (greater than 90.1%) less than or equal to 10000Da, has polypeptide total protein content of 88.6% by dry weight and has ash content of 7.2%.
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Description

technical field

[0001] The invention belongs to the enzymatic production technology of marine bioengineering, and relates to a process for preparing antioxidant peptide powder by enzymatically hydrolyzing skipjack. Background technique

[0002] At present, the aquatic product processing industry has become an important part of my country's fishery economy. Especially the processing and export of canned aquatic products has provided a large number of employment opportunities for our country, greatly increased personal income and foreign exchange sources. In the production of canned aquatic products, especially canned fish, a large amount of by-products such as cooking liquid and head and tail sections will be produced. In my country's fish processing, there is a total of nearly one million tons of cooking liquid that needs to be developed urgently every year, and low-value processing as feed or discarded head By-products such as the tail section amount to several million tons...

Claims

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