41results about How to "Promote maturity" patented technology

This invention provides a combined delivery system for eosinophil cationic protein (ECP) and interleukin-4 (IL-4) and its applications, belonging to the field of biopharmaceutical preparation technology. The combined biodelivery system includes a matrix loaded with eosinophil cationic protein (ECP) and an auxiliary carrier loaded with interleukin-4 (IL-4). This invention utilizes the difference in degradation kinetics between hydrogels and microspheres to achieve a stepwise sequential delivery strategy: rapid release of ECP in the early stage (0-7 days) to stabilize the endothelial barrier, followed by sustained release of IL-4 in the later stage (7-28 days) to induce M2 polarization and recruit smooth muscle. Experiments have demonstrated that the combined delivery system can significantly improve the inflammatory microenvironment after myocardial infarction, synergistically promote functional vascular maturation and inhibit ventricular remodeling, providing a novel treatment option for the repair of ischemic heart disease.

This invention provides an oyster peptide and its application in enhancing immunity. The oyster peptide has a novel amino acid structure. The oyster peptide can enhance the activity of immune cells, promote the proliferation of immune cells, promote the development and maturation of immune organs, regulate the in vivo immune environment, promote the secretion of immunoglobulins, and improve humoral immunity.

The present application relates to the field of biotechnology, and more particularly to the application of tomato SlWRKY39 and SlWRKY40 in regulating tomato fruit ripening. The present application discloses a method for promoting tomato fruit ripening, which comprises simultaneously knocking out SlWRRY39 and SlWRKY40 genes to promote the ripening of tomato fruits. The present application also simultaneously provides a gene editing vector for realizing the promotion of tomato fruit ripening, which is pCAMBIA1300-pYAO:Cas9-(SlWRKY39+SlWRKY40). It is found through fruit phenotype observation and average ripening time statistics that the simultaneous knockout of SlWRKY39 and SlWRKY40 promotes the ripening of tomato fruits.

The invention discloses modified chickpea starch and a preparation method and application thereof, and belongs to the technical field of food processing.The modified chickpea starch is obtained by adding oat beta glucan into chickpea starch, then conducting pretreatment on the chickpea starch, conducting extrusion treatment on the chickpea starch through a double-screw extruder, and cooling, drying and smashing an extruded sample; wherein the addition amount of the oat beta glucan is 3 to 15 percent; the temperature of the extrusion treatment is 80-120 DEG C. The chickpea starch is subjected to compound modification by adopting a twin-screw extrusion technology in cooperation with oat beta glucan, and a starch semi-crystalline layer is deeply destroyed to realize deep regulation and control of a multi-scale structure; meanwhile, the oat beta glucan and the starch form a hydrogen bond network, so that the problem of excessive modification of chemical modification is avoided, and the thermal stability, the shear resistance and the aging resistance of the starch are remarkably improved; no chemical reagent participates in the whole process, no chemical residue risk exists, and the method has the advantages of continuous production, large treatment capacity, low energy consumption and the like.

The application discloses an LDH-based vaccine adjuvant and a preparation method and application thereof, and belongs to the technical field of aluminum adjuvant preparation of vaccines, and the preparation method comprises the following steps: mixing at least one of soluble magnesium salt, soluble zinc salt, soluble manganese salt and soluble calcium salt with soluble aluminum salt, and then performing a co-precipitation reaction with a sodium hydroxide aqueous solution in a colloid mill device, and performing crystallization to obtain LDH nanosheets; dispersing the LDH nanosheets in a PBS solution, adding a hepatitis B surface antigen molecule drug, uniformly mixing, and then performing centrifugation to obtain the LDH-based vaccine adjuvant. The LDH-based vaccine adjuvant can significantly promote the maturation of DC cells and the activation of T cells, and the total IgG level reflects the overall humoral immune response intensity of the body to vaccines or pathogens; the LDH-based vaccine adjuvant forms a "storage library" at an injection site to store antigens and slowly release the antigens, and the purpose of long-time continuous stimulation of the immune system in the body is achieved.

The invention discloses a water source conservation function evaluation method suitable for a high and cold frozen soil region, and belongs to the field of water source conservation function evaluation.The method comprises the steps that a monitoring network is laid in a drainage basin of the high and cold frozen soil region, and monitoring data are collected; the monitoring data comprises meteorological data, frozen soil data, hydrological data and vegetation and underlying surface data; improving the VIC frozen soil hydrological model by using the monitoring data; determining constraint conditions of the improved VIC permafrost hydrological model based on a permafrost characteristic water source conservation mechanism, and simulating each component of water balance of the high and cold permafrost region drainage basin by using the improved VIC permafrost hydrological model; and based on each component of water balance of the drainage basin of the high and cold frozen soil region, calculating the water conservation amount by using a water balance equation, and obtaining the water conservation capability according to the water conservation amount to complete water conservation function evaluation. According to the invention, key processes such as frozen soil thermal conductivity overestimation abnormity and accumulated snow sublimation effect supplementation are eliminated, and the simulation precision of the hydrothermal transmission process is improved.

The invention belongs to the technical field of sea grass culture, and particularly relates to an eelgrass seed after-ripening method. The invention provides an eelgrass seed after-ripening method which comprises the following steps: collecting eelgrass reproductive branches, and removing main branches of the eelgrass reproductive branches to obtain eelgrass reproductive branch branches with Buddhist bracts; hanging the eelgrass reproduction branch branches in seawater for after-ripening to obtain eelgrass seeds. According to the method, the main branches of the eelgrass reproduction branches are removed, nutrient resource consumption of redundant tissues to the branches is reduced, more favorable conditions are created for development of the Buddha bracts and the seeds, the perfectness ratio and the seed maturity of the Buddha bracts in the after-ripening period are increased by 30% or above, the seed recovery rate is increased to 90% or above, the seed activity reaches 100%, the cost is reduced by 30% compared with that of a conventional method, and the method is suitable for large-scale popularization and application. After sowing, the seedling establishment rate exceeds 50%, the overall effect far exceeds that of a conventional collection and after-ripening mode, and the method is easy to operate, low in cost and more suitable for large-scale repair and reconstruction of the seaweed bed.

The utility model belongs to the technical field of high-temperature flow field pressure testing, and discloses a water-cooled wall surface static pressure probe provided with multiple cooling loops and used for measuring a high-temperature flow field of an aero-engine combustion chamber. The device is characterized by comprising a pressure sensing hole, a fixed external thread, an octagonal prism positioning block, a fixed internal thread, a sensor mounting cavity, eight water outlet channels and eight water inlet channels. Compared with an existing water-cooling pressure probe, the probe can measure the wall surface static pressure of a flow field under the condition that the interference on the flow field is as small as possible, and meanwhile, the water-cooling pressure probe can adjust the cooling effect of each area of the probe. When the wall surface static pressure of a high-temperature flow field of a combustion chamber of an aero-engine is actually measured, the water-cooled wall surface static pressure probe is fixed in a static pressure hole which is formed in the wall surface of the combustion chamber in advance, a pressure sensor is mounted in the probe, and the water-cooled wall surface static pressure probe can measure the wall surface static pressure in the combustion chamber in a 2500K high-temperature environment through the calibration of a shock tube. And the frequency response of the probe exceeds 80 kHz.

The invention discloses an aerodynamic heat management configuration design method for a large-airspace and wide-speed-domain hypersonic aircraft. The aerodynamic heat management configuration design method comprises the steps that a reference aerodynamic layout is determined, and appearance design oriented to heat flow distribution optimization is carried out; establishing a thermal environment database through multi-working-condition numerical simulation; dividing the surface of the aircraft into thermal management areas of different grades based on the thermal environment database; configuring a thermal protection material and designing a gradient thermal protection structure for each thermal management area to realize conformal integration with the reference aerodynamic layout; the integrity and reliability of the integrated structure under the heat / force load are verified through heat / structure coupling simulation analysis; and carrying out performance evaluation and iterative optimization, and if the design indexes are not met, returning to corresponding steps for optimization until the design is converged. Integrated collaborative design and closed-loop iterative optimization of aerodynamic configuration and thermal management performance are realized from a design source, and the problems of overweight of a thermal protection system, poor thermal environment adaptability and low design efficiency caused by a traditional serial method are effectively solved.

The present application relates to the technical field of biological medicine, in particular to a gemcitabine-indoleamine 2,3-dioxygenase inhibitor conjugate, a prodrug nanomicelle, a preparation method and an application. The conjugate covalently connects gemcitabine and NLG919 through a connecting arm containing a triazole structure, forms an amphiphilic molecule with a hydrophilic-hydrophobic structure, and can self-assemble into stable small-size nanomicelles in an aqueous solution. The nanomicelle has a pH and enzyme dual-responsive drug release property, can improve the stability and delivery efficiency of gemcitabine, promote cell uptake, and enhance the synergistic antitumor effect of chemotherapy and immunomodulation. The present application also provides an application of the nanomicelle in the preparation of an antitumor drug.

The present application relates to a kind of surface enhanced raman nanoparticles and fentanyl substance detection method, belong to the field of drug safety detection.The surface enhanced raman nanoparticles is noble metal nanoparticle, the noble metal nanoparticle is preferably gold nanobiscake.The fentanyl substance detection method uses the surface enhanced raman nanoparticles of the present application to carry out raman detection.The surface enhanced raman nanoparticles and fentanyl substance detection method can quickly, non-destructively detect trace fentanyl substance in the sample to be measured, and has very good application prospect in public safety detection.

The present application belongs to the technical field of biological medicine, and particularly relates to an esterase-responsive PEGylated conjugate containing a sulfonamide group and a preparation method and application thereof. The esterase-responsive PEGylated conjugate comprises: a PEGylated hydrophilic segment; an esterase-cleavable linker arm, the linker arm comprising at least one ester bond; a copper death sensitization functional structure containing a sulfonamide group; wherein the copper death sensitization functional structure containing a sulfonamide group is covalently connected to the PEGylated hydrophilic segment through the esterase-cleavable linker arm. The esterase-responsive PEGylated conjugate containing a sulfonamide group provided by the present application can self-assemble to form nanoparticles (EsCu@NPs), and through an esterase response mechanism, L-BSO and copper ions are accurately released at a tumor site, GSH is efficiently consumed and copper death is induced, thereby triggering immunogenic cell death (ICD), promoting the maturation of dendritic cells and CD8 + T cell infiltration, and significantly enhancing the therapeutic effect of an anti-PD-1 immune checkpoint inhibitor.

This invention discloses a method for promoting somatic embryogenesis and maturation in Cryptomeria japonica, belonging to the field of somatic embryogenesis technology. The method involves using immature seed embryos as explants to induce embryogenic callus; transferring the proliferated embryogenic callus to a somatic embryogenesis induction medium for further culture to obtain somatic embryos; transferring the somatic embryos to a somatic embryogenesis maturation medium for dark culture to obtain mature cotyledonary embryos; transferring the mature cotyledonary embryos to a somatic embryogenesis basal medium for light culture to obtain somatic embryonic seedlings; and hardening off the seedlings before transplanting to obtain regenerated plants. The results of this application show that embryogenic callus can be stably obtained from different families, with high somatic embryogenesis maturation capacity and rate, enabling the cultivation of a large number of high-quality seedlings with stable genetic traits in a short time; avoiding the phenotypic segregation problem caused by gene recombination during seed propagation, and ensuring that offspring plants inherit the superior characteristics of the parents.

The invention discloses an ROS responsive antibody nano-drug conjugate as well as a preparation method and application thereof, and belongs to the field of biological medicines. The conjugate comprises a nano-drug carrier, a TIM-3 blocking antibody and a VISTA blocking antibody, wherein the nano-drug carrier is encapsulated with a first chemotherapeutic drug and a second chemotherapeutic drug which can be subjected to cascade reaction, and the TIM-3 blocking antibody and the VISTA blocking antibody are covalently connected to the surface of the carrier through ROS responsive linkers. After reaching a tumor site, the conjugate can respond to a high-level ROS release antibody of a tumor microenvironment, synchronously block TIM-3 and VISTA immune checkpoints, and relieve immunosuppression; the carried chemotherapeutic drug is subjected to cascade reaction in tumor cells to induce immunogenic death and release tumor antigens and danger signals. Tumor cascade killing and antibody effective blocking synergism can effectively remodel an immunosuppression microenvironment, activate and enhance anti-tumor immune response, and realize efficient tumor killing. The conjugate has the advantages of precise targeting, synergistic interaction and good biological safety, and provides a new strategy for tumor immunotherapy.

The invention relates to the technical field of microorganisms, in particular to a multifunctional lactobacillus paracasei strain capable of promoting growth and development and application of the multifunctional lactobacillus paracasei strain. The Latin name of the multifunctional Lactobacillus paracasei strain is Lacticaseibacillus paracasei ZG1, the preservation number of the multifunctional Lactobacillus paracasei strain is CGMCC No.36863, the preservation date of the multifunctional Lactobacillus paracasei strain is December 5, 2025, and the preservation unit of the multifunctional Lactobacillus paracasei strain is China General Microbiological Culture Collection Center. Experiments prove that the multifunctional lactobacillus paracasei strain has the functions of promoting neurocognitive development, immune system maturation and bone growth, and can be added into an infant probiotic preparation to play a role in promoting growth and development. Compared with existing probiotics, the lactobacillus paracasei ZG1 has a multi-target and multi-system synergistic promotion function on growth and development, is expected to replace a traditional mixed probiotic scheme, and fundamentally solves the core problems of single function, mixed antagonism and the like of most of existing probiotics.

This invention discloses an oxygen-catalyzing gel microsphere for regulating mitochondrial energy metabolism and its application in tissue repair. The microsphere comprises a calcium alginate hydrogel framework and multiple separate micro-reaction chambers embedded within the calcium alginate hydrogel framework. Each micro-reaction chamber independently contains ozone oil and graphene. This invention constructs a near-infrared responsive oxygen-catalyzing gel microsphere, achieving structured regulation of local oxygen supply and effective remodeling of cellular metabolic states. The system utilizes a dual-emulsion-microfluidic electrospray strategy to construct a spatially separated multi-chamber structure. Under near-infrared stimulation, the Gr-mediated photothermal-electronic synergy promotes the in-situ, efficient, and relatively mild conversion of ozone to oxygen, providing new insights for the design of hypoxia-related bone regeneration and other tissue repair materials.

This invention discloses a non-destructive mango separation intelligent agricultural harvesting device and method. The harvesting device includes a walking mechanism, a lifting mechanism, a horizontal sliding mechanism, a robotic arm, an energy-induced end effector, and a flexible buffer collection mechanism. The energy-induced end effector includes a flexible fruit clamping component, an end recognition camera, a micro-vibration spectral generation and analysis module, and a multi-frequency resonant energy-induced module. The micro-vibration spectral generation and analysis module is used to perform non-contact detection of the fruit stalk, obtain the spectral response of the fruit stalk cells, and compare it with the in vitro difficulty fingerprint spectrum to determine whether the optimal harvesting period has been reached. If so, a decision command containing the inherent resonant frequency and phase characteristics of the fruit stalk is output. The multi-frequency resonant energy-induced module emits a resonant wave with the same frequency but opposite phase as the inherent vibration frequency of the fruit stalk, and uses the phase cancellation interference principle to instantaneously weaken the cell binding force at the molecular level to achieve non-destructive separation.

A gene for increasing the number of tomato fruits and a method thereof, wherein the nucleotide sequence of the gene is shown in Fig. Seq No. 1. The present application is obtained by obtaining the complete coding sequence of the gene from tomato SlGRF The gene is amplified by PCR technology, then the target fragment is connected to the pBG-GFP vector, the plant is transformed by using the agrobacterium infection method, the high expression plant is obtained, and the high expression plant is analyzed, and the results show that the high expression of the gene can increase the fruit setting number of tomato.

The application discloses a pH-responsive deformable double-targeting nano-protein and a preparation method and application thereof, and the method comprises the following steps: after carboxylated mannose is activated through an EDC / NHS system, the carboxylated mannose is reacted with an albumin solution, and mannose-modified albumin is obtained through dialysis purification; the mannose-modified albumin is mixed with an inhibitor IRG1-IN-1, and IRG1-IN-1 is loaded through hydrophobic interaction to obtain mannose albumin nanoparticles; ferritin is used as a carrier, and oxaliplatin is loaded through a solvent displacement method to obtain ferritin nanoparticles; CHO-PEG2k-CHO is used as a connecting agent, and the mannose albumin nanoparticles and the ferritin nanoparticles are crosslinked through a Schiff base reaction to form large-size pH-responsive double-targeting nano-proteins. The double-targeting separable characteristics of the nano-proteins can realize specific treatment, kill tumor cells, interfere with immune cell metabolism, block metabolic immunosuppression, and promote anti-tumor immunotherapy.

The invention relates to the field of antibodies useful in therapeutic and diagnostic applications targeting cd127, the alpha chain of the il7 receptor, and in particular provides humanized monoclonal antibodies against cd127, especially human cd127, their therapeutic uses and diagnostic applications.

This invention relates to a myocardial repair patch based on multi-material printing, its preparation, and its application, belonging to the field of bio-3D printing and regenerative medicine technology. The invention uses PLCL and GelMA hydrogel loaded with active cardiomyocytes as bio-inks, and introduces gelatin gel as a heat buffer material. An orthogonal network structure is constructed using multi-material bio-3D printing technology to prepare a myocardial repair patch with excellent dynamic adaptability to the myocardial interface and good biocompatibility. The myocardial injury repair patch prepared by this invention achieves a structure-function integrated design through the synergistic effect of the three materials, significantly overcoming the limitations of single materials in terms of mechanical properties, biological function, or thermal compatibility. The mechanical properties of the myocardial injury repair patch prepared by this invention can precisely match those of natural myocardium, while possessing high cell activity, effectively inhibiting fibrosis, improving myocardial function, and achieving true active repair. Furthermore, the process conditions are controllable, demonstrating good translational potential.

The application discloses a preparation method of a drug sustained-release hydrogel support and a three-dimensional osteogenic induction application of hiPSCs. Firstly, a CHIR99021@BSA-DEX@PLGA time-sequential double sustained-release composite microsphere of a core-shell structure is prepared, then the freeze-dried GelMA powder is dissolved in a PBS solution containing a photoinitiator LAP, the CHIR99021@BSA-DEX@PLGA time-sequential double sustained-release composite microsphere is added after stirring and cooling, and a drug-loaded hydrogel precursor is obtained; the precursor is injected into a mold and irradiated by a specific wavelength light source to initiate free radical polymerization of a methacryl group into glue, and a drug-loaded GelMA porous hydrogel support is obtained after demolding, freezing and drying; the drug-loaded GelMA porous support is covalently grafted with VN polypeptide through EDC / NHS chemical coupling to obtain a VN-GelMA drug-loaded porous hydrogel support. The application simulates the signal time sequence regulation law in the embryonic bone formation process and significantly improves the osteogenic differentiation efficiency and mineralization quality by constructing a biomimetic three-dimensional osteogenic microenvironment. The VN polypeptide enhances the specific recognition of stem cells and significantly improves the three-dimensional cell compatibility. The application can reduce the dependence on exogenous induction factors and is safe, low in cost and stable in induction.

The invention belongs to the technical field of inorganic materials, and particularly discloses a method for producing water glass and co-producing sylvite by utilizing biomass, which comprises the following steps: (a) carrying out oxygen-enriched combustion on the biomass at 650-800 DEG C; (b) mixing biomass ash obtained by combustion with water, carrying out heating pretreatment, and carrying out solid-liquid separation while the mixture is hot after the heating pretreatment is ended, so as to obtain clarified filtrate and a biomass ash filter cake; (c) mixing the biomass ash filter cake with an alkali solution, dissolving, and performing solid-liquid separation to obtain biomass ash and water glass; and (d) continuously recycling the clarified filtrate until crystals are separated out, continuously cooling and crystallizing, and carrying out solid-liquid separation to obtain the sylvite. According to the method, the directional conversion mode of the potassium element in the biomass is achieved, the potassium element is directly extracted, the treated biomass ash enters the rear end to be prepared into the water glass, synchronous production of the bio-based water glass and the high-purity potassium salt is directly achieved, the process is simple, the cost is low, and an innovative path is provided for industry green transformation.

The application discloses a planting method for delaying flowering and fruit setting of citrus, and belongs to the technical field of citrus planting. The planting method comprises the following steps: S1, promoting autumn shoot budding and maturation: before autumn shoot budding, pruning existing branches, and applying nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer, and after the new shoots are cut, spraying 0.2-0.4% potassium dihydrogen phosphate+200-300 ml / L paclobutrazol; S2, inhibiting spring shoot budding: before spring shoot budding, avoiding rain and controlling water, and keeping the soil dry; S3, promoting early summer shoot budding: before early summer shoot budding, pruning existing branches, and applying nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer; and S4, fruit protection: respectively spraying a fruit protection agent during the last flowering period and after the first physiological fruit drop period ends. The method can delay the maturity and marketing time of the citrus, and prolongs the fresh fruit supply period by about 90 days compared with the conventional planting mode.

The application discloses a gene for promoting tomato fruit ripening and a regulation method thereof, wherein the nucleotide sequence of the gene is shown as Seq No. 1. SlDCD2 The complete coding sequence of the gene is obtained from tomatoes, a target is designed, the target is connected to a CRIPSR-Cas9 vector, a plant is transformed by using an agrobacterium infection method, a gene editing plant is obtained, and the gene editing plant is analyzed, and the result shows that the gene editing can accelerate the ripening of tomato fruits.

The application discloses application of MaCLE16 gene and / or synthetic short peptide MaCLE16p in regulation of fruit ripening of plants and belongs to the technical field of genetic engineering. The nucleotide sequence of the MaCLE16 gene is shown as SEQ ID NO. 3; and the amino acid sequence of the synthetic short peptide MaCLE16p is RLVPTGPNPLHN. The experiment proves that the banana fruit ripening time is shortened by 2-3 days, and the soluble solid content and the pulp hardness are respectively increased by 15% and 20% when the MaCLE16 gene is overexpressed. The further synthesized core short peptide MaCLE16p can dose-dependently accelerate the banana fruit yellowing, and the effect is consistent with that of the gene overexpression, and there is no chemical residue risk. The application first discloses the regulation effect of the CLE gene in fruit ripening, provides a new strategy for developing a green and safe ripening agent, and has significant industrial application value.

The invention belongs to the field of avionics, relates to an aircraft airborne system design technology, and provides a novel software-defined airborne system architecture and an implementation method thereof.The software-defined airborne system architecture adopts a layered design mode, functions, performance and resources of each layer in an airborne system are flexibly configured and reconstructed through software definition, and the software-defined airborne system architecture has the advantages that the software-defined airborne system architecture is simple in structure and convenient to implement. Comprising a hardware platform layer, a hardware abstraction layer, an operating system resource layer, a platform function service layer and an application layer. The novel software-defined airborne system architecture solves the problems that a traditional airborne system is low in integration level and poor in flexibility, hardware generalization, software modularization and agile iteration of functions are achieved, and the novel software-defined airborne system architecture has excellent universality and expansibility; and the objective of optimal integrated balance of higher performance, safety and economical efficiency of a full-life-cycle product of the airborne system is met.

A vegetable circulating fermentation system comprises a plurality of fermentation tanks and at least one mixing tank, each fermentation tank is used for fermentation of only one kind of vegetable, the top of the fermentation tank is provided with a feeding port and a spraying pipe, the lower end of the spraying pipe extends into the fermentation tank, the bottom of the fermentation tank is provided with a discharging port and a liquid discharge pipe, the liquid discharge pipes of the plurality of fermentation tanks are connected with the mixing tank, the height position of the mixing tank is lower than that of the fermentation tank, the mixing tank is connected with the spraying pipe through a conveying pipeline, and a water pump is arranged on the conveying pipeline. The scheme helps to accelerate the maturity of the vegetables, meets the requirements of rich strains and tastes, and meets the requirement of convenient discharging.

The application discloses a kind of pyricularia oryzae antagonistic streptomyces fermentation preparation, preparation method and application, belong to microbial fermentation and biological pesticide field, which comprises the following steps: screening the inhibitory rate of more than 80% of pyricularia oryzae starting strain of streptomyces, ribosome engineering is reformed by rpsL gene K88E directional point mutation, the value is greater than 5000 ug / mL in the optimized fermentation medium of carbon-nitrogen ratio 25:1~30:1, corn syrup 0.5%~1% culture makes potency, and the preparation of suspension preparation containing viable bacteria and metabolites is prepared by suspending liquid and supernatant concentrate compound, and the comprehensive prevention and cure effect of the preparation in field reaches more than 75%, which can replace chemical pesticide.

The application relates to the technical field of cell differentiation and the technical field of myocardial tissue regeneration medicine, in particular to an induced culture medium and method for quickly obtaining a spontaneously beating myocardial ball, wherein the myocardial cells of a human induced pluripotent stem cell (hiPSC) source obtained through co-culture with hMSCs are combined with a differentiation culture medium, so that the myocardial cell clones harvested on day 7 are more independent and naturally separated from other non-myocardial cells, the myocardial clones can be quickly screened and purified through physical and non-enzyme reagents, the step of medium screening and purification in the traditional method is omitted, and time and differentiation cost are saved.