77results about How to "Promote maturity" patented technology

The invention discloses a Carnot battery system based on phase change cold storage and energy storage and a multi-mode operation method thereof, the Carnot battery system comprises a vapor compression refrigeration unit, a phase change cold storage device and a power cycle unit, in a charging stage, the vapor compression refrigeration unit is adopted to generate cold energy by using off-peak power; in the energy storage stage, cold energy prepared by the steam compression refrigeration unit is stored in a high-density mode through the phase change cold storage device; in the discharging stage, the stored cold energy serves as a cold source, external low-temperature waste heat serves as a heat source, a power circulation unit is adopted, meanwhile, the cold energy and the hot energy are utilized for heat-work conversion, and electric energy is released; according to the Carnot battery system provided by the invention, the round-trip efficiency and the energy storage density of an energy storage system can be improved, the energy storage cost is reduced, and large-scale long-time energy storage is facilitated; according to the multi-mode operation method provided by the invention, through dispatching application of the phase change cold storage device and the charge-discharge cycle, different types of low-temperature heat sources can be fully utilized, and the cold electricity energy consumption requirement of a user side is met.

The invention discloses low-drunkenness type Baijiu distiller's yeast and a preparation method and application thereof. The prepared distiller's yeast can be used for producing baijiu, the physical and chemical indexes of the baijiu are good, fusel oil is reduced by 30%-57%, the metabolism speed of yeast is controlled by controlling the saccharification speed, and the generation of the fusel oil is reduced; meanwhile, lactic acid bacteria and bacillus subtilis of the distiller's yeast and bacteria cultured in a high-temperature stage can effectively inhibit the path of fusel oil metabolized by yeast, generation of fusel oil is further reduced, total ester and total acid are slightly increased basically, the content of total dibasic acid is increased by 10 times, and the content of dibasic acid diethyl ester is increased by 2 times. After the wine liquid is aged, drunkenness detection is carried out, and the comprehensive drunkenness Q is 0.6815-0.8325 and is far lower than a variety of commercially available white spirits (the comprehensive drunkenness is greater than or equal to 1).

The invention provides a PCELL layout design optimization method and device, a medium and equipment, and is applied to the technical field of chip design. Flexible layout drawing is carried out by acquiring the PCELL containing the process parameters and the graph adjustable parameters, so that the layout structure parameters of the current PCELL are obtained. A three-dimensional device simulation model is constructed by using the parameters, and a simulation compact model is created by using an artificial neural network algorithm to describe the electrical behavior of the device. And verifying the electrical performance trend of the three-dimensional device simulation model by using the PCELL and the simulation compact model. And when the verification is passed, further verifying whether the module test circuit meets a preset PPA index. And if yes, fixing the structural parameters of the current PCELL layout. According to the method, process and design requirements are integrated through a construction and parameterization method, and simulation and verification of closed-loop feedback are utilized, so that the design precision and the process maturity are improved, the development period is shortened, and the reliability and efficiency of the process are enhanced.

The invention relates to the technical field of cellular immunotherapy, in particular to application of efonidipine in preparation of a dendritic cell immunologic adjuvant. The invention provides an application of efonidipine in preparation of a dendritic cell immunologic adjuvant. It is found that efonidipine can promote maturation, migration and antigen presentation functions of DCs, and the homing ability of DCs and CD8 + T cell activation are remarkably improved. The activation aspect of efonidipine on DCs comprises improvement of expression of maturation indexes CD80, CD40 and CD86, expression of a migration index CCR7, activation of CD8 + T cells by antigen presentation and the like. The efonidipine used in the invention is a drug approved by FDA for clinical treatment of cardiovascular diseases, the application of the efonidipine in immunotherapy is found, new use of old drugs is realized, and the efonidipine has great economic value.

The invention discloses an antigen peptide polymer vesicle tumor vaccine as well as a preparation method and application thereof. The antigen peptide polymer vesicle tumor vaccine is obtained by assembling and cross-linking a polymer pre-grafted with antigen peptide and a block polymer and loading an immunologic adjuvant through electrostatic interaction. The vesicle system disclosed by the invention has the advantages of small size, simplicity and controllability in preparation, excellent biocompatibility, high in-vivo circulation stability, targeting of main immune organs, intracellular delivery of antigen peptides and the like. The vesicle nano vaccine displaying the antigen peptide on the surface and encapsulating the immunologic adjuvant can promote dendritic cell maturation and antigen presentation, improve the specific response of mice to tumor-associated antigens, effectively inhibit tumor growth, remarkably prolong the lifetime of tumor-bearing mice and induce a strong immune protection effect, and is high in safety and easy to popularize. A multifunctional'ready-to-use 'nano vaccine concept is embodied, a flexible platform is provided, and different peptide antigens and / or adjuvants can be used for treating different cancers.

The invention discloses a low-salt rapid bamboo shoot fermentation method, which adopts an inoculated fermentation mode, uses lactobacillus plantarum and lactobacillus brevis as leavening agents, and uses fresh bamboo shoots as raw materials. The preparation method comprises the following steps: selecting fresh bamboo shoots with high tenderness, peeling off bamboo shoot shells, cutting off bases, cleaning, and cutting into bamboo shoot slices with uniform sizes; cooking in boiling water for 5 minutes, fishing out, and airing to remove moisture; preparing water with the salt concentration of 2% and the glucose concentration of 2%, boiling the water, and airing the water for later use to obtain a fermentation feed liquid; adding the air-dried fresh bamboo shoot slices and the prepared material liquid into a bag which is scalded by boiling water and cooled according to a solid-to-liquid ratio of 1: 4, and then adding a lactic acid bacteria leavening agent into the fermentation bag for sealed fermentation; and placing the fermentation bag in an environment with a temperature of 30 DEG C for fermentation for 5 days. The prepared fermented bamboo shoots are short in fermentation period, low in nitrite content, mellow and normal in sourness, rich in flavor and high in sensory quality and food safety, and can better meet industrial and consumer requirements.

The invention belongs to the field of tumor drugs, and discloses a radioactive hydrogel based on crosslinking of sodium alginate and metal ions and a preparation method and application thereof.The preparation method of the radioactive hydrogel comprises the following steps that lutetium chloride and metal ions are mixed, and a metal ion mixed solution is obtained; doping 177Lu radioactive isotope into the obtained metal ion mixed solution to obtain a metal ion cross-linking agent; and injecting a metal ion cross-linking agent into the sodium alginate solution under a vortex condition, centrifuging, and discarding the supernatant to obtain the radioactive hydrogel. The radioactive hydrogel (177Lu-RH) provided by the invention has good in-tumor colonization capability, can be directly injected into a tumor to realize local radiotherapy, has good radioactive stability, and can be stably retained at a tumor part for more than 120 hours, so that the stability of radioactive labeling in a treatment process is ensured.

The invention provides a high-pressure-resistant low-permeability hydrogen rubber sealing material and a preparation method thereof, and the sealing material comprises the following components by weight: 100 parts of ethylene propylene rubber; 4-8 parts of an active agent; 1-3 parts of an anti-aging agent; 20 to 50 parts of a nanometer material; 50 to 60 parts of a filling reinforcing agent; 2-5 parts of a coupling agent; 3-4 parts of a vulcanizing agent dicumyl peroxide; 1 to 2 parts of trialkenyl triisourea serving as an auxiliary vulcanizing agent; 3-8 parts of vulcanized resin; the invention also provides a preparation method of the high-pressure-resistant low-permeability hydrogen rubber sealing material. The preparation method comprises the following steps: pre-mixing, feeding and mixing, adding cross-linking components, standing and curing, hot-pressing and vulcanizing, post-curing and the like. The sealing material disclosed by the invention realizes high-level balance in the aspects of hydrogen permeation barrier, high-pressure burst resistance, wide-temperature interval tolerance and the like, and can effectively meet the sealing requirements of a hydrogen storage system and a high-pressure hydrogen conveying pipeline of a current hydrogen fuel automobile; the material has great advantages in the aspects of raw material cost, manufacturing process and performance stability.

The invention provides a Ilex plant germination accelerating, sowing and seedling raising method, and relates to the technical field of Ilex seedling raising. The Ilex plant germination accelerating, sowing and seedling raising method comprises the following steps of fruit collection, after-ripening treatment, natural stratification treatment, germination accelerating and soaking, seed germination and sowing seedling stage management. Through optimization measures in the aspects of after-ripening treatment, germination accelerating soaking, natural stratification treatment, sand storage and soil storage composite germination accelerating and germination combined with proper seedling stage management after planting and the like, the germination rate of ilex plant seeds and the growth quality of seedlings are remarkably improved, and meanwhile the method has the advantages of being environmentally friendly and recycling resources. The method is an efficient, economical and environment-friendly seedling raising method.

A composite microbial agent for promoting the decomposition of corn stalks, the composite microbial agent is composed of Myrotheca verrucosus ( Myrothecium verrucaria )、Aspergillus niger( Aspergillus niger ) Trichoderma harzianum ) and Trichoderma reesei ( Trichoderma reesei ). In the present invention, the compound of Myrocera verrucosus, Aspergillus niger, Trichoderma harzianum and Trichoderma reesei produces a synergistic effect, effectively improves the degradation rate of lignocellulose in straw, and the degradation rates of cellulose, hemicellulose and lignin are 39.38%, 37.67% and 31.04% respectively, and the humic acid content in the compost reaches 114.94 mg / g, and the stability of humic acid is effectively improved, and the maturity of the compost is effectively improved, and the GI of the compost reaches 91.39%.

The invention discloses a ternary carrier-free nanoparticle as well as a preparation method and application thereof. The ternary carrier-free nanoparticles comprise nanoparticles which are formed by the chlorine e6, the lonidamine and the RG108 through an intermolecular interaction force. The nanoparticles can improve effective absorption and accumulation of drugs in tumors, can induce tumor specific pyroptosis and immunogenic effect under laser irradiation, and can promote maturation of antigen presenting cells and recruitment and activation of cytotoxic T lymphocytes, thereby starting adaptive anti-tumor immunity.

This invention provides a combined delivery system for eosinophil cationic protein (ECP) and interleukin-4 (IL-4) and its applications, belonging to the field of biopharmaceutical preparation technology. The combined biodelivery system includes a matrix loaded with eosinophil cationic protein (ECP) and an auxiliary carrier loaded with interleukin-4 (IL-4). This invention utilizes the difference in degradation kinetics between hydrogels and microspheres to achieve a stepwise sequential delivery strategy: rapid release of ECP in the early stage (0-7 days) to stabilize the endothelial barrier, followed by sustained release of IL-4 in the later stage (7-28 days) to induce M2 polarization and recruit smooth muscle. Experiments have demonstrated that the combined delivery system can significantly improve the inflammatory microenvironment after myocardial infarction, synergistically promote functional vascular maturation and inhibit ventricular remodeling, providing a novel treatment option for the repair of ischemic heart disease.

The present invention discloses a robot for intelligent monitoring of fruit maturity, which belongs to the technical field of fruit maturity monitoring, and comprises a robot body, a mechanical arm, and a clamping mechanism; a controller is installed on the robot body; the mechanical arm is installed on the robot body; the mechanical arm comprises a main support arm, a secondary support wall, a first cylinder and a second cylinder in sequence, the main support arm is rotatably connected to the robot body, and the secondary support wall is rotatably connected to the main support arm. The present invention obtains the gas containing ethylene in the bag by inserting a needle into the bag, first injecting gas and then exhausting gas, and utilizes the principle that fruits with different maturity secrete different ethylene. By detecting the ethylene concentration in the gas, the maturity of the fruit in the paper bag can be intelligently monitored, and the development of robot harvesting technology can be effectively promoted; at the same time, for unripe fruits, high-concentration ethylene gas can be injected into the bag to ripen them, which accelerates the maturity of the fruit, shortens the harvesting time, and saves manpower and material resources.

The invention discloses a planting method for delaying flowering and fruit setting of citrus, and belongs to the technical field of citrus planting. The planting method comprises the following steps that S1, autumn shoots are promoted to sprout and mature, before the autumn shoots sprout, existing branches are trimmed, nitrogenous fertilizer, phosphate fertilizer and potash fertilizer are applied, and 0.2-0.4% monopotassium phosphate and 200-300 ml / L paclobutrazol are sprayed after new shoots are self-trimmed; s2, spring shoot germination inhibition: before spring shoot germination, rain sheltering and water control are carried out, and soil drought is kept; s3, sprouting of early summer shoots is promoted, before sprouting of the early summer shoots, existing branches are trimmed, and nitrogenous fertilizer, phosphate fertilizer and potash fertilizer are applied; s4, fruit retention: spraying a fruit retention agent during the end-flowering period and after the first physiological fruit drop period. The method can delay the maturing and marketing time of citrus, and prolongs the fresh fruit supply period by about 90 days compared with a conventional planting mode.

This invention provides an oyster peptide and its application in enhancing immunity. The oyster peptide has a novel amino acid structure. The oyster peptide can enhance the activity of immune cells, promote the proliferation of immune cells, promote the development and maturation of immune organs, regulate the in vivo immune environment, promote the secretion of immunoglobulins, and improve humoral immunity.

The invention belongs to the technical field of nondestructive testing, and relates to an ultrasonic testing and test block manufacturing method for an additive and subtractive composite manufacturing component. According to the method, an additive and subtractive composite manufacturing component manufactured by an additive forming complex structure on a forge piece base body is taken as an object, and reference blocks for ultrasonic detection are manufactured aiming at a forging forming area, an additive / forging transition area and an additive forming distinguishing area respectively; and proper detection directions, sensitivity adjustment modes and the like are respectively selected according to the forming process and structural characteristics of each region, so that comprehensive detection of the internal defects of the additive and subtractive composite manufacturing component is realized. According to the method, the problem of inaccurate detection results caused by anisotropy and structure nonuniformity of the additive manufacturing material is solved, a comprehensive and effective detection means is provided for additive and subtractive composite manufacturing components through regional detection, and a guarantee is provided for improving the quality of additive manufacturing products.

The present application relates to the field of biotechnology, and more particularly to the application of tomato SlWRKY39 and SlWRKY40 in regulating tomato fruit ripening. The present application discloses a method for promoting tomato fruit ripening, which comprises simultaneously knocking out SlWRRY39 and SlWRKY40 genes to promote the ripening of tomato fruits. The present application also simultaneously provides a gene editing vector for realizing the promotion of tomato fruit ripening, which is pCAMBIA1300-pYAO:Cas9-(SlWRKY39+SlWRKY40). It is found through fruit phenotype observation and average ripening time statistics that the simultaneous knockout of SlWRKY39 and SlWRKY40 promotes the ripening of tomato fruits.

The invention discloses modified chickpea starch and a preparation method and application thereof, and belongs to the technical field of food processing.The modified chickpea starch is obtained by adding oat beta glucan into chickpea starch, then conducting pretreatment on the chickpea starch, conducting extrusion treatment on the chickpea starch through a double-screw extruder, and cooling, drying and smashing an extruded sample; wherein the addition amount of the oat beta glucan is 3 to 15 percent; the temperature of the extrusion treatment is 80-120 DEG C. The chickpea starch is subjected to compound modification by adopting a twin-screw extrusion technology in cooperation with oat beta glucan, and a starch semi-crystalline layer is deeply destroyed to realize deep regulation and control of a multi-scale structure; meanwhile, the oat beta glucan and the starch form a hydrogen bond network, so that the problem of excessive modification of chemical modification is avoided, and the thermal stability, the shear resistance and the aging resistance of the starch are remarkably improved; no chemical reagent participates in the whole process, no chemical residue risk exists, and the method has the advantages of continuous production, large treatment capacity, low energy consumption and the like.

The application discloses an LDH-based vaccine adjuvant and a preparation method and application thereof, and belongs to the technical field of aluminum adjuvant preparation of vaccines, and the preparation method comprises the following steps: mixing at least one of soluble magnesium salt, soluble zinc salt, soluble manganese salt and soluble calcium salt with soluble aluminum salt, and then performing a co-precipitation reaction with a sodium hydroxide aqueous solution in a colloid mill device, and performing crystallization to obtain LDH nanosheets; dispersing the LDH nanosheets in a PBS solution, adding a hepatitis B surface antigen molecule drug, uniformly mixing, and then performing centrifugation to obtain the LDH-based vaccine adjuvant. The LDH-based vaccine adjuvant can significantly promote the maturation of DC cells and the activation of T cells, and the total IgG level reflects the overall humoral immune response intensity of the body to vaccines or pathogens; the LDH-based vaccine adjuvant forms a "storage library" at an injection site to store antigens and slowly release the antigens, and the purpose of long-time continuous stimulation of the immune system in the body is achieved.

The invention discloses a water source conservation function evaluation method suitable for a high and cold frozen soil region, and belongs to the field of water source conservation function evaluation.The method comprises the steps that a monitoring network is laid in a drainage basin of the high and cold frozen soil region, and monitoring data are collected; the monitoring data comprises meteorological data, frozen soil data, hydrological data and vegetation and underlying surface data; improving the VIC frozen soil hydrological model by using the monitoring data; determining constraint conditions of the improved VIC permafrost hydrological model based on a permafrost characteristic water source conservation mechanism, and simulating each component of water balance of the high and cold permafrost region drainage basin by using the improved VIC permafrost hydrological model; and based on each component of water balance of the drainage basin of the high and cold frozen soil region, calculating the water conservation amount by using a water balance equation, and obtaining the water conservation capability according to the water conservation amount to complete water conservation function evaluation. According to the invention, key processes such as frozen soil thermal conductivity overestimation abnormity and accumulated snow sublimation effect supplementation are eliminated, and the simulation precision of the hydrothermal transmission process is improved.

The invention belongs to the technical field of sea grass culture, and particularly relates to an eelgrass seed after-ripening method. The invention provides an eelgrass seed after-ripening method which comprises the following steps: collecting eelgrass reproductive branches, and removing main branches of the eelgrass reproductive branches to obtain eelgrass reproductive branch branches with Buddhist bracts; hanging the eelgrass reproduction branch branches in seawater for after-ripening to obtain eelgrass seeds. According to the method, the main branches of the eelgrass reproduction branches are removed, nutrient resource consumption of redundant tissues to the branches is reduced, more favorable conditions are created for development of the Buddha bracts and the seeds, the perfectness ratio and the seed maturity of the Buddha bracts in the after-ripening period are increased by 30% or above, the seed recovery rate is increased to 90% or above, the seed activity reaches 100%, the cost is reduced by 30% compared with that of a conventional method, and the method is suitable for large-scale popularization and application. After sowing, the seedling establishment rate exceeds 50%, the overall effect far exceeds that of a conventional collection and after-ripening mode, and the method is easy to operate, low in cost and more suitable for large-scale repair and reconstruction of the seaweed bed.

The utility model discloses a tracking-free condensation unit and photovoltaic power generation combined structure, and solves the problems that the change range of the incident angle of light rays is large when the sun runs from sunrise to sunset, and sunlight is difficult to efficiently and stably collect without adopting a tracking device through the unique design of a condensation optical system. And meanwhile, through the effective combination of the light-gathering heat-collecting unit and the photovoltaic power generation panel, a combined structure of the tracking-free light-gathering heat-collecting unit and photovoltaic power generation is formed, the integrated operation of light-gathering heat-collecting and photovoltaic power generation is realized, and the comprehensive utilization efficiency of sunlight is improved. According to the utility model, a plurality of effective combinations can be formed with relatively mature photovoltaic technology, photo-thermal technology and light guide illumination technology, so that the device is suitable for various concentrating light fields from low power to high power and from small power to large power, can be widely applied to the fields of solar concentrating heat collection, illumination, power generation and the like, and is wide in application scene.

The utility model discloses a rubber product forming die which comprises an operation table, a die table and a feeding table, the feeding table, the die table and the operation table are fixedly connected in sequence from left to right, and baffles are fixedly connected to the two sides of the feeding table, the two sides of the die table and the two sides of the operation table respectively. Two pulley bearings are fixedly connected between the top sides of the baffles, the two pulley bearings penetrate through and are rotationally connected with a first pulley and a second pulley correspondingly, a transmission rope is connected between the first pulley and the second pulley in an engaged mode, one end of the transmission rope is fixedly connected with a fixing disc, and the fixing disc is fixedly connected with a rotating shaft. A rolling column is arranged and rolled at the other end of the transmission rope; according to the utility model, rubber raw materials can be pre-processed, so that the processing efficiency is higher in the rubber pressing production and processing process.

The utility model belongs to the technical field of high-temperature flow field pressure testing, and discloses a water-cooled wall surface static pressure probe provided with multiple cooling loops and used for measuring a high-temperature flow field of an aero-engine combustion chamber. The device is characterized by comprising a pressure sensing hole, a fixed external thread, an octagonal prism positioning block, a fixed internal thread, a sensor mounting cavity, eight water outlet channels and eight water inlet channels. Compared with an existing water-cooling pressure probe, the probe can measure the wall surface static pressure of a flow field under the condition that the interference on the flow field is as small as possible, and meanwhile, the water-cooling pressure probe can adjust the cooling effect of each area of the probe. When the wall surface static pressure of a high-temperature flow field of a combustion chamber of an aero-engine is actually measured, the water-cooled wall surface static pressure probe is fixed in a static pressure hole which is formed in the wall surface of the combustion chamber in advance, a pressure sensor is mounted in the probe, and the water-cooled wall surface static pressure probe can measure the wall surface static pressure in the combustion chamber in a 2500K high-temperature environment through the calibration of a shock tube. And the frequency response of the probe exceeds 80 kHz.

The invention discloses an aerodynamic heat management configuration design method for a large-airspace and wide-speed-domain hypersonic aircraft. The aerodynamic heat management configuration design method comprises the steps that a reference aerodynamic layout is determined, and appearance design oriented to heat flow distribution optimization is carried out; establishing a thermal environment database through multi-working-condition numerical simulation; dividing the surface of the aircraft into thermal management areas of different grades based on the thermal environment database; configuring a thermal protection material and designing a gradient thermal protection structure for each thermal management area to realize conformal integration with the reference aerodynamic layout; the integrity and reliability of the integrated structure under the heat / force load are verified through heat / structure coupling simulation analysis; and carrying out performance evaluation and iterative optimization, and if the design indexes are not met, returning to corresponding steps for optimization until the design is converged. Integrated collaborative design and closed-loop iterative optimization of aerodynamic configuration and thermal management performance are realized from a design source, and the problems of overweight of a thermal protection system, poor thermal environment adaptability and low design efficiency caused by a traditional serial method are effectively solved.

The present application relates to the technical field of biological medicine, in particular to a gemcitabine-indoleamine 2,3-dioxygenase inhibitor conjugate, a prodrug nanomicelle, a preparation method and an application. The conjugate covalently connects gemcitabine and NLG919 through a connecting arm containing a triazole structure, forms an amphiphilic molecule with a hydrophilic-hydrophobic structure, and can self-assemble into stable small-size nanomicelles in an aqueous solution. The nanomicelle has a pH and enzyme dual-responsive drug release property, can improve the stability and delivery efficiency of gemcitabine, promote cell uptake, and enhance the synergistic antitumor effect of chemotherapy and immunomodulation. The present application also provides an application of the nanomicelle in the preparation of an antitumor drug.

The invention relates to a method for strengthening the surface of a cathode roller, which is characterized in that turning, grinding, shot blasting and polishing are taken as the basis, a new machining method is formed by combining new working procedures, the coaxiality of the surface of a titanium cylinder of the machined cathode roller is less than or equal to 0.05 mm, the circle run-out degree is less than or equal to 0.05 mm, the straightness is less than or equal to 0.05 mm, and the surface roughness is less than Ra 0.3; the mirror surface effect is achieved, and surface defects such as cross grains, cross grains, chromatic aberration and bright spots are avoided; the stress corrosion resistance of the cathode roller titanium cylinder is improved, the time of surface defects in the electrolytic copper foil production process is greatly delayed, the service life of the cathode roller is prolonged, the production efficiency of the cathode roller is improved, the effect superior to that of an existing machining method is achieved, the corrosion resistance of the cathode roller is remarkably improved, and the service cycle of the cathode roller is greatly prolonged. And the product meets the use requirements of high-end copper foils with high tensile strength and high elongation. According to the method, the required equipment is high in maturity, the equipment purchase cost and the processing cost are not obviously increased, and the method has good operability and economical efficiency.

The present application relates to a kind of surface enhanced raman nanoparticles and fentanyl substance detection method, belong to the field of drug safety detection.The surface enhanced raman nanoparticles is noble metal nanoparticle, the noble metal nanoparticle is preferably gold nanobiscake.The fentanyl substance detection method uses the surface enhanced raman nanoparticles of the present application to carry out raman detection.The surface enhanced raman nanoparticles and fentanyl substance detection method can quickly, non-destructively detect trace fentanyl substance in the sample to be measured, and has very good application prospect in public safety detection.

The present application belongs to the technical field of biological medicine, and particularly relates to an esterase-responsive PEGylated conjugate containing a sulfonamide group and a preparation method and application thereof. The esterase-responsive PEGylated conjugate comprises: a PEGylated hydrophilic segment; an esterase-cleavable linker arm, the linker arm comprising at least one ester bond; a copper death sensitization functional structure containing a sulfonamide group; wherein the copper death sensitization functional structure containing a sulfonamide group is covalently connected to the PEGylated hydrophilic segment through the esterase-cleavable linker arm. The esterase-responsive PEGylated conjugate containing a sulfonamide group provided by the present application can self-assemble to form nanoparticles (EsCu@NPs), and through an esterase response mechanism, L-BSO and copper ions are accurately released at a tumor site, GSH is efficiently consumed and copper death is induced, thereby triggering immunogenic cell death (ICD), promoting the maturation of dendritic cells and CD8 + T cell infiltration, and significantly enhancing the therapeutic effect of an anti-PD-1 immune checkpoint inhibitor.

The invention relates to a design method for an integrated system of a manned spacecraft assembly. The design method comprises the steps that a system and cabin section two-stage assembly is set up; the system generally unifies construction specifications, development processes and production and insurance requirements, and puts forward universal design requirements of each cabin section; according to the system, the top layer design and the system function design of a manned spacecraft assembly are integrally completed, task functions and an index system are decomposed into cabin sections, and interfaces between the cabin sections are defined; overall decomposition of the cabin sections is carried out, functional and technical requirements on subsystems are issued, and interfaces between the subsystems are defined; the subsystem completes subsystem-level design, decomposes and issues function and technical requirements for single machines, and defines interfaces between the single machines; a single machine completes design and development of products according to unified construction specifications and technical requirements. The method can be popularized and applied to design of complex system spacecraft assemblies in various fields, and it is ensured that functions of all cabin sections are fused and coordinated, the product generalization rate is high, the technical state convergence speed is high, and the product maturity is high.