271results about How to "Promote maturity" patented technology

The invention provides a coal gangue organic fertilizer and a preparation method thereof, and belongs to the technical field of fertilizers. The coal gangue organic fertilizer is prepared by uniformly mixing modified coal gangue, forestry and agricultural residues and a microbial agent, performing high-temperature aerobic composting and aging. Organic components of the coal gangue and nutrient elements such as nitrogen, phosphorus, potassium, calcium and the like required by plant growth are fully utilized, the carrier effect of the coal gangue is also played, and the coal gangue organic fertilizer prepared from the modified coal gangue has a relatively high decomposition degree and is greatly beneficial to seed growth. According to the invention, on one hand, the problems of large stockpiling land occupation and environmental pollution of coal gangue, forestry and agricultural residues and other solid wastes are relieved, and on the other hand, the prepared organic fertilizer can turn waste into wealth and realize solid waste recycling.

The invention relates to the field of recycling of solid waste resources. The invention particularly relates to a method for synergistically recovering failed lithium iron phosphate black powder and a failed platinum-containing catalyst. The invention discloses a method for synergistically recovering failed lithium iron phosphate black powder and a failed platinum-containing catalyst. The method comprises the following steps: A, vacuum reduction smelting; b, oxidizing and dissolving; c, carbon thermal reduction; and D, ammonium salt precipitation and calcination. The method for recovering the platinum group metal does not need to add a trapping agent, has the advantages of being high in resource utilization rate, good in economic benefit and short in process, and effectively reduces the influence of solid waste resources on the environment.

The invention discloses a Carnot battery system based on phase change cold storage and energy storage and a multi-mode operation method thereof, the Carnot battery system comprises a vapor compression refrigeration unit, a phase change cold storage device and a power cycle unit, in a charging stage, the vapor compression refrigeration unit is adopted to generate cold energy by using off-peak power; in the energy storage stage, cold energy prepared by the steam compression refrigeration unit is stored in a high-density mode through the phase change cold storage device; in the discharging stage, the stored cold energy serves as a cold source, external low-temperature waste heat serves as a heat source, a power circulation unit is adopted, meanwhile, the cold energy and the hot energy are utilized for heat-work conversion, and electric energy is released; according to the Carnot battery system provided by the invention, the round-trip efficiency and the energy storage density of an energy storage system can be improved, the energy storage cost is reduced, and large-scale long-time energy storage is facilitated; according to the multi-mode operation method provided by the invention, through dispatching application of the phase change cold storage device and the charge-discharge cycle, different types of low-temperature heat sources can be fully utilized, and the cold electricity energy consumption requirement of a user side is met.

The invention discloses low-drunkenness type Baijiu distiller's yeast and a preparation method and application thereof. The prepared distiller's yeast can be used for producing baijiu, the physical and chemical indexes of the baijiu are good, fusel oil is reduced by 30%-57%, the metabolism speed of yeast is controlled by controlling the saccharification speed, and the generation of the fusel oil is reduced; meanwhile, lactic acid bacteria and bacillus subtilis of the distiller's yeast and bacteria cultured in a high-temperature stage can effectively inhibit the path of fusel oil metabolized by yeast, generation of fusel oil is further reduced, total ester and total acid are slightly increased basically, the content of total dibasic acid is increased by 10 times, and the content of dibasic acid diethyl ester is increased by 2 times. After the wine liquid is aged, drunkenness detection is carried out, and the comprehensive drunkenness Q is 0.6815-0.8325 and is far lower than a variety of commercially available white spirits (the comprehensive drunkenness is greater than or equal to 1).

The invention provides a porcine oocyte in-vitro maturation culture solution additive and application thereof, and belongs to the field of embryo engineering, and the additive contains taurine. The additive also contains sodium pyruvate, cysteine, glucose, NaHCO3, fetal calf serum, estradiol, pregnant horse serum gonadotropin and human chorionic gonadotropin. When the porcine oocyte in-vitro maturation culture solution added with the additive is used for culturing porcine oocytes in vitro, the ROS content in the porcine oocytes can be remarkably reduced, the oxidation resistance of the porcine oocytes can be improved, and the mitochondrial distribution of the oocytes can be improved, so that the development of the oocytes can be improved; the maturation of porcine oocytes and the development of early embryos are promoted. When the technology is applied to the pig assisted reproduction technology, the success rate and quality of embryo engineering can be improved; the pig oocyte in-vitro maturation culture solution added with the additive can improve the premature embryo division rate in parthenogenetic activation of the pig oocytes.

The invention provides a PCELL layout design optimization method and device, a medium and equipment, and is applied to the technical field of chip design. Flexible layout drawing is carried out by acquiring the PCELL containing the process parameters and the graph adjustable parameters, so that the layout structure parameters of the current PCELL are obtained. A three-dimensional device simulation model is constructed by using the parameters, and a simulation compact model is created by using an artificial neural network algorithm to describe the electrical behavior of the device. And verifying the electrical performance trend of the three-dimensional device simulation model by using the PCELL and the simulation compact model. And when the verification is passed, further verifying whether the module test circuit meets a preset PPA index. And if yes, fixing the structural parameters of the current PCELL layout. According to the method, process and design requirements are integrated through a construction and parameterization method, and simulation and verification of closed-loop feedback are utilized, so that the design precision and the process maturity are improved, the development period is shortened, and the reliability and efficiency of the process are enhanced.

The invention relates to the technical field of cellular immunotherapy, in particular to application of efonidipine in preparation of a dendritic cell immunologic adjuvant. The invention provides an application of efonidipine in preparation of a dendritic cell immunologic adjuvant. It is found that efonidipine can promote maturation, migration and antigen presentation functions of DCs, and the homing ability of DCs and CD8 + T cell activation are remarkably improved. The activation aspect of efonidipine on DCs comprises improvement of expression of maturation indexes CD80, CD40 and CD86, expression of a migration index CCR7, activation of CD8 + T cells by antigen presentation and the like. The efonidipine used in the invention is a drug approved by FDA for clinical treatment of cardiovascular diseases, the application of the efonidipine in immunotherapy is found, new use of old drugs is realized, and the efonidipine has great economic value.

The invention provides an application of a PavERF110 gene in regulating and controlling the ripening direction of sweet cherry fruits, and belongs to the technical field of molecular breeding. The nucleotide sequence of the PavERF110 gene is as shown in SEQ ID NO.1, and the amino acid sequence of the encoded protein of the PavERF110 gene is as shown in SEQ ID NO.2. The invention also provides an overexpression vector and a VIGS gene silencing vector constructed by the gene, a construction method of the overexpression vector and the VIGS gene silencing vector, and a recombinant microorganism containing the vectors. Engineering bacteria containing related carriers are injected and infected at the equator of the fruits 25 days after the sweet cherries blossom, and it is found that overexpression of the PavERF110 gene can accelerate fruit coloring and increase the expression level of the mature related genes. New technical support and theoretical basis are provided for sweet cherry fruit quality improvement and ripening regulation, market requirements can be met, and meanwhile understanding of a fruit ripening transcription regulation mechanism is deepened.

The invention provides an automatic driving vehicle high-precision positioning system and method based on AOA and laser radar, a medium and equipment, and the method comprises the steps: S1, receiving a Bluetooth signal transmitted by a vehicle through an AOA module, and calculating the arrival angle of the signal; s2, combining the arrival angle and vehicle position information provided by a GPS module to obtain position prediction; s3, laser pulses are emitted through a laser radar module, reflected signals are received, and point cloud data of the surrounding environment are obtained; s4, comparing the point cloud data with a prior positioning map, determining the number of a grid where the vehicle is located, and obtaining a positioning result through online normal distribution transformation; and S5, based on the information provided by the GPS module and the inertia module, the calculation module optimizes the positioning result to obtain a precision positioning result. The method does not affect the positioning accuracy and stability even in an underground scene, improves the positioning precision, reduces the calculation resource demand, can adapt to a complex environment, and also reduces the dependence on the initial position.

The invention discloses a cultivation and production technology of a flue-cured tobacco variety Yuzhou 201. The cultivation and production technology comprises the following steps: (1) selecting a tobacco field; (2) performing winter ploughing and ridging; (3) sowing time; (4) scientific transplanting; (5) balanced fertilization; (6) field management; (7) tobacco field plant protection; (8) harvesting; and (9) tobacco leaf baking. The tobacco leaves planted and harvested through the method are high in quality, sensory evaluation results show that the smoking quality of the tobacco leaves meets industrial requirements, and the overall sensory quality of the tobacco leaves planted through the method shows that the tobacco leaves are rich and comfortable in fragrance, good in fragrance quality, obvious in sweetness, high in fragrance amount, high in smoke concentration and moderate in strength; the high-quality strong-flavor tobacco leaf raw material is high in quality, low in irritation, comfortable in aftertaste, small in offensive odor and high in overall smoking quality, and meets the requirements of high-grade cigarettes.

The invention discloses an antigen peptide polymer vesicle tumor vaccine as well as a preparation method and application thereof. The antigen peptide polymer vesicle tumor vaccine is obtained by assembling and cross-linking a polymer pre-grafted with antigen peptide and a block polymer and loading an immunologic adjuvant through electrostatic interaction. The vesicle system disclosed by the invention has the advantages of small size, simplicity and controllability in preparation, excellent biocompatibility, high in-vivo circulation stability, targeting of main immune organs, intracellular delivery of antigen peptides and the like. The vesicle nano vaccine displaying the antigen peptide on the surface and encapsulating the immunologic adjuvant can promote dendritic cell maturation and antigen presentation, improve the specific response of mice to tumor-associated antigens, effectively inhibit tumor growth, remarkably prolong the lifetime of tumor-bearing mice and induce a strong immune protection effect, and is high in safety and easy to popularize. A multifunctional'ready-to-use 'nano vaccine concept is embodied, a flexible platform is provided, and different peptide antigens and / or adjuvants can be used for treating different cancers.

The invention discloses a planting management method of flue-cured tobacco variety Heiluo No.1, and relates to the technical field of flue-cured tobacco planting, comprising the following steps: (1) tobacco field selection; (2) performing winter ploughing and ridging; (3) sowing time; (4) scientific transplanting; (5) balanced fertilization; (6) field management; (7) tobacco field plant protection; (8) harvesting; the tobacco leaves planted and harvested through the method are high in quality, the smoking quality requirement of the tobacco leaves is reflected through a sensory evaluation result, and the overall sensory quality of the tobacco leaves planted through the method shows that the tobacco leaves are rich and comfortable in fragrance, good in fragrance quality, obvious in sweetness, moderate in fragrance amount, medium in smoke concentration, moderate in strength and small in irritation; the tobacco is a high-quality tobacco raw material which is comfortable in aftertaste, light in offensive odor and high in overall smoking quality and meets the requirements of high-grade cigarettes.

The invention relates to a pathological pulmonary fibrosis model and a construction method and application thereof. The pathological pulmonary fibrosis model provided by the invention is good in stability and controllability and high in maturity, not only can better simulate the function and structural characteristics of pulmonary alveoli-capillary barrier, but also has the pathological characteristics and immune microenvironment of pulmonary fibrosis lesions, has the advantage of high simulation degree, and has good application prospects in simulation and research of pulmonary fibrosis diseases. Good application prospects are realized in the aspects of drug detection, screening, evaluation, research and development and the like.

The invention relates to the technical field of grape planting, in particular to a grape nutrition regulation-based early-maturing high-quality light and simplified production method, which comprises the following steps of: regulating nutrition distribution in a germination period, and taking moisture-preserving and rain-sheltering measures on the ground and branches; regulating and controlling nutrient distribution in the flowering period to control branch growth, and ensuring the fruit setting rate based on pinching measures; management is conducted in the fruit expanding period, on the basis of vigorous growth control combined with high photosynthetic management, the photosynthetic efficiency of leaves is ensured, soil and leaf fertilization are adjusted, and it is ensured that nutrient supply meets the fruit expanding requirement; nutrition in the color changing period is regulated and controlled, fruit color changing is promoted based on trunk girdling, specific fertilizer is sprayed to leaf surfaces, and it is ensured that the fruit ripening and color changing process is accelerated; management is conducted in the acid-removing and sugar-increasing period, and the fruit acidity is gradually reduced based on regulation and control of nutrient distribution and fertilization measures. According to the method, the efficiency of early-maturing, high-quality and light-weight production of grapes is improved.

The invention discloses a low-salt rapid bamboo shoot fermentation method, which adopts an inoculated fermentation mode, uses lactobacillus plantarum and lactobacillus brevis as leavening agents, and uses fresh bamboo shoots as raw materials. The preparation method comprises the following steps: selecting fresh bamboo shoots with high tenderness, peeling off bamboo shoot shells, cutting off bases, cleaning, and cutting into bamboo shoot slices with uniform sizes; cooking in boiling water for 5 minutes, fishing out, and airing to remove moisture; preparing water with the salt concentration of 2% and the glucose concentration of 2%, boiling the water, and airing the water for later use to obtain a fermentation feed liquid; adding the air-dried fresh bamboo shoot slices and the prepared material liquid into a bag which is scalded by boiling water and cooled according to a solid-to-liquid ratio of 1: 4, and then adding a lactic acid bacteria leavening agent into the fermentation bag for sealed fermentation; and placing the fermentation bag in an environment with a temperature of 30 DEG C for fermentation for 5 days. The prepared fermented bamboo shoots are short in fermentation period, low in nitrite content, mellow and normal in sourness, rich in flavor and high in sensory quality and food safety, and can better meet industrial and consumer requirements.

The invention belongs to the field of tumor drugs, and discloses a radioactive hydrogel based on crosslinking of sodium alginate and metal ions and a preparation method and application thereof.The preparation method of the radioactive hydrogel comprises the following steps that lutetium chloride and metal ions are mixed, and a metal ion mixed solution is obtained; doping 177Lu radioactive isotope into the obtained metal ion mixed solution to obtain a metal ion cross-linking agent; and injecting a metal ion cross-linking agent into the sodium alginate solution under a vortex condition, centrifuging, and discarding the supernatant to obtain the radioactive hydrogel. The radioactive hydrogel (177Lu-RH) provided by the invention has good in-tumor colonization capability, can be directly injected into a tumor to realize local radiotherapy, has good radioactive stability, and can be stably retained at a tumor part for more than 120 hours, so that the stability of radioactive labeling in a treatment process is ensured.

The invention provides application of baicalin in preparation of a medicine for treating streptococcus agalactiae induced mastitis, and relates to the technical field of medicine. A mouse is selected as an animal model to research the intervention effect and mechanism of the baicalin on the streptococcus agalactiae induced mouse mastitis inflammatory response, and it is clear that the baicalin can play a role in protecting the mouse mammary gland by regulating and controlling an NLRP3 inflammatory small body pathway; a reference basis is provided for treating the cow mastitis caused by streptococcus agalactiae infection by applying the baicalin.

The invention discloses a method for optimizing the maturation effect of cattle and sheep oocytes through CNP pretreatment, and relates to the technical field of biological breeding, the method comprises the following steps: S1, selecting and treating donor cells; s2, collection of oocytes; s3, in-vitro maturation culture of the oocytes: after the pre-maturation treatment, transferring the oocytes into a maturation culture solution for continuous culture under the in-vitro culture condition of air containing 5% CO2, keeping the culture temperature of the oocytes within 1-20 hours at 38.5-39 DEG C, and keeping the culture temperature of the oocytes within 20-28 hours at 39-39.5 DEG C; s4, culturing and screening reconstructed embryos; s5, preparing and transplanting a receptor animal; and S6, performing subsequent monitoring and management. According to the method, the culture temperature is kept at 38.5-39 DEG C within 1-20 hours, the culture temperature is kept at 39-39.5 DEG C within 20-28 hours, a series of enzymes and signal channels in the oocytes can be effectively activated by accurately controlling the culture temperature, and the survival rate and the development quality of the oocytes are improved.

The invention relates to the technical field of biology, in particular to application of tomatoes SlWRKY39 and SlWRKY40 in regulation and control of tomato fruit maturity. The invention discloses a method for promoting maturation of tomato fruits. According to the method, SlWRRY39 and SlWRKY40 genes are knocked out at the same time, and maturation of the tomato fruits is promoted. The invention also provides a gene editing vector for promoting the maturation of the tomato fruits, wherein the gene editing vector is pCAMBIA1300-pYAO: Cas9-(SlWRKY39 + SlWRKY40). Through fruit phenotype observation and average maturation time statistics, it is found that simultaneous knockout of SlWRKY39 and SlWRKY40 promotes maturation of tomato fruits.

The invention relates to the field of mangrove forest ecological system restoration, in particular to a mangrove forest tidal flat-based mud dice culture method and application thereof. The method comprises the following steps: (a) by taking an estuary mangrove forest tidal flat as a breeding base, arranging mud dice apartments on bare beaches inside and outside the mangrove forest; wherein the mud dice apartment is filled with culture soil; the top end of the mud dice apartment is exposed out of the beach surface; (b) putting mud dice seedlings into the mud dice apartment for cultivation and management and protection; and (c) harvesting the mud dices after the mud dices are mature. According to the mud dice cultivation method based on the mangrove forest tidal flat, a movable circulating container cultivation mode is adopted, mud dice is cultivated on bare beaches inside and outside the mangrove forest, and the mud dice cultivation method combining mangrove forest restoration and tidal flat efficient utilization is created. The method can be used for rapidly repairing a damaged mangrove forest ecological system, the quality of tidal flat soil is improved in the efficient cultivation process of mud dices, and sustainable utilization of the tidal flat is achieved.

The invention provides an artificial breeding method of spinibarbus capito, which is suitable for Dongting Lake in Hunan Province and four water basins of Hunan, Zi, active yuan and halite, and comprises the steps of parent fish rearing, artificial spawning induction and artificial incubation, and spawning can be carried out for 1-2 times in one year. In the spinibarbus capito parent fish rearing process, puffed floating fish feed is used as a main material and properly matched with green feed, animal bait is added for feeding 2 months before winter, accumulation of fish body fat can be guaranteed so that the fish can smoothly pass the winter, vitamin E, vitamin C and flowing water stimulation are supplemented in the feed before antenatal, and ovary development and ovum maturation can be effectively promoted; the artificial spawning induction adopts a two-needle injection method, the spawning induction efficiency is high, and the fertilization rate is high; through comparison of different hatching devices, the hatching effect of the hatching frame is best, the hatching rate reaches 80% or above, the hatching barrel is inferior, and the small net cage is worst. By means of the artificial propagation method, large-scale production of spinibarbus capito can be achieved, and the method is of great significance in improving the economic benefits of farmers and popularizing the spinibarbus capito artificial propagation technology.

The invention provides a high-pressure-resistant low-permeability hydrogen rubber sealing material and a preparation method thereof, and the sealing material comprises the following components by weight: 100 parts of ethylene propylene rubber; 4-8 parts of an active agent; 1-3 parts of an anti-aging agent; 20 to 50 parts of a nanometer material; 50 to 60 parts of a filling reinforcing agent; 2-5 parts of a coupling agent; 3-4 parts of a vulcanizing agent dicumyl peroxide; 1 to 2 parts of trialkenyl triisourea serving as an auxiliary vulcanizing agent; 3-8 parts of vulcanized resin; the invention also provides a preparation method of the high-pressure-resistant low-permeability hydrogen rubber sealing material. The preparation method comprises the following steps: pre-mixing, feeding and mixing, adding cross-linking components, standing and curing, hot-pressing and vulcanizing, post-curing and the like. The sealing material disclosed by the invention realizes high-level balance in the aspects of hydrogen permeation barrier, high-pressure burst resistance, wide-temperature interval tolerance and the like, and can effectively meet the sealing requirements of a hydrogen storage system and a high-pressure hydrogen conveying pipeline of a current hydrogen fuel automobile; the material has great advantages in the aspects of raw material cost, manufacturing process and performance stability.

The invention provides a Ilex plant germination accelerating, sowing and seedling raising method, and relates to the technical field of Ilex seedling raising. The Ilex plant germination accelerating, sowing and seedling raising method comprises the following steps of fruit collection, after-ripening treatment, natural stratification treatment, germination accelerating and soaking, seed germination and sowing seedling stage management. Through optimization measures in the aspects of after-ripening treatment, germination accelerating soaking, natural stratification treatment, sand storage and soil storage composite germination accelerating and germination combined with proper seedling stage management after planting and the like, the germination rate of ilex plant seeds and the growth quality of seedlings are remarkably improved, and meanwhile the method has the advantages of being environmentally friendly and recycling resources. The method is an efficient, economical and environment-friendly seedling raising method.

The invention relates to the field of recycling of rare and noble metal secondary resources. The invention particularly relates to a method for recovering platinum group metals and rare earth in a spent automobile three-way catalyst. The invention relates to a method for recovering platinum group metal and rare earth in a spent automobile three-way catalyst. The method comprises the following steps: A, crushing and fine grinding; b, uniformly mixing and briquetting; C, vacuum high-temperature smelting; d, fine crushing and magnetic separation; and E, acid leaching. The method has the advantages of simple process flow, high resource recovery rate, environmental protection and the like, and has wide industrial application prospects.

The invention relates to a pulmonary fibrosis model and a construction method and application thereof. The pulmonary fibrosis model provided by the invention is good in stability and controllability and high in maturity, can better simulate functions and structural characteristics of pulmonary alveoli-capillary barriers, has the advantage of high simulation, and has a good application prospect in the aspects of disease simulation, drug detection, screening, evaluation, research and development and the like.

A composite microbial agent for promoting the decomposition of corn stalks, the composite microbial agent is composed of Myrotheca verrucosus ( Myrothecium verrucaria )、Aspergillus niger( Aspergillus niger ) Trichoderma harzianum ) and Trichoderma reesei ( Trichoderma reesei ). In the present invention, the compound of Myrocera verrucosus, Aspergillus niger, Trichoderma harzianum and Trichoderma reesei produces a synergistic effect, effectively improves the degradation rate of lignocellulose in straw, and the degradation rates of cellulose, hemicellulose and lignin are 39.38%, 37.67% and 31.04% respectively, and the humic acid content in the compost reaches 114.94 mg / g, and the stability of humic acid is effectively improved, and the maturity of the compost is effectively improved, and the GI of the compost reaches 91.39%.

The invention belongs to the field of biological medicine and materials, and particularly relates to a nano vaccine for promoting antigen cross presentation and a preparation method and application thereof, the method comprises the following steps: inducing tumor cell immunogenicity death to obtain a tumor cell immunogenicity death product; a polylactic acid-glycolic acid copolymer is mixed with alpha-galactosylceramide to serve as a carrier, a tumor cell immunogenicity death product, racimod and endosome-lysosome escape promoting peptide are wrapped, and the nano vaccine is prepared. The method for inducing immunogenic death of the tumor cells comprises the following steps: co-incubating the tumor cells and the iridium composite oligoarginine polypeptide and / or irradiating the tumor cells with the photosensitizer by laser. The nano vaccine has efficient antigen cross presentation capability, and can stimulate expression of dendritic cell co-stimulation signals and secretion of cell factors in multiple ways, so that the biological effect of synergistically activating cellular immunity is achieved.

The invention relates to the technical field of aerobic treatment biology, and discloses an aerobic treatment biological synergistic process which comprises the following steps: adding sewage or organic waste or biogas residue / biogas slurry into a first flora culture tank; adding a PH regulator to regulate the PH value; and introducing gas into the first flora culture tank by using an air blower for stirring. According to the aerobic treatment biological synergistic process, the targeted dominant flora can be autonomously cultured according to different treatment objects, and the autonomously cultured flora is automatically added by monitoring various parameters, so that the aerobic reaction process is accelerated, the reaction degree is improved, the reaction time of aerobic treatment is shortened, the process treatment effect is improved, and the treatment cost is reduced. For a sewage aerobic biological treatment process, the COD (Chemical Oxygen Demand) removal rate can be improved; for a process for producing an organic fertilizer through aerobic treatment of organic wastes, the decomposition degree of the organic fertilizer can be improved, the quality of the organic fertilizer can be improved, manual operation is not needed, full-automatic control operation can be realized, meanwhile, dependence on external bacteria or flora is avoided, and the method is worthy of popularization.

An in vitro method for constructing a human vaginal epithelial model, which uses two stages of submerged culture and air-liquid surface culture to form a multilayered in vitro 3D model. During the submerged culture process, a low concentration of Ca 2+ (0.7~1.5mM) prevents the differentiation of vaginal epithelial cells; vitamin D is added to ensure that there are enough epithelial cells to maintain cell differentiation in the subsequent gas-liquid culture stage. In the gas-liquid interface culture stage, all-trans retinoic acid and 17 β-estradiol are added to the differentiation medium, among which all-trans retinoic acid can inhibit the continuous keratinization of vaginal epithelial cells and prevent excessive keratinization to form a stratum corneum. 17 β-estradiol enhances the barrier function of the vaginal epithelial model. Therefore, the joint use of all-trans retinoic acid and 17 β-estradiol can ensure that the epithelial layer of the vaginal epithelial model cannot differentiate into a mature stratum corneum, but still has a certain barrier function.

The invention discloses a ternary carrier-free nanoparticle as well as a preparation method and application thereof. The ternary carrier-free nanoparticles comprise nanoparticles which are formed by the chlorine e6, the lonidamine and the RG108 through an intermolecular interaction force. The nanoparticles can improve effective absorption and accumulation of drugs in tumors, can induce tumor specific pyroptosis and immunogenic effect under laser irradiation, and can promote maturation of antigen presenting cells and recruitment and activation of cytotoxic T lymphocytes, thereby starting adaptive anti-tumor immunity.