291 results about "Trichoderma harzianum" patented technology

The invention relates to the technical field of fertilizer preparation, and discloses an ecological balance fertilizer for yield increase and disease resistance of peanuts and a preparation method of the ecological balance fertilizer. The preparation process comprises the following steps: constructing a PLGA core containing trichoderma harzianum spores and magnesium carbonate through a micro-fluidic chip low-temperature water-in-oil emulsion method; coating bacillus amyloliquefaciens and potassium silicate with ultraviolet-induced cross-linked temperature-sensitive hydrogel to form a middle layer; loading rhizobium by using aminated mesoporous S < iO2 >, and spraying and assembling an enzyme response shell through a fluidized bed; finally, the hydroxyapatite nanorod and a binder are combined and granulated and formed through a fluidized bed. Precise controlled release of functional bacteria and nutrients is realized by constructing a temperature-sensitive-enzyme response type release system, and the activity of a fungicide is guaranteed to be preserved by virtue of trichoderma harzianum-rhizobium synergistic interaction and silicon bond linkage soil remediation in combination with a low-temperature fluidized bed process, so that a'fertilizer-bacterium-soil 'ecological cycle system is finally constructed.

The invention relates to the technical field of microbial agents, in particular to a composite microbial agent and application of the composite microbial agent in prevention and treatment of forest root diseases, and the composite microbial agent comprises the following components in parts by weight: 15-25 parts of Trichoderma harzianum ATCC 20847; 10 to 20 parts of bacillus subtilis ATCC 605, 10 to 20 parts of bacillus subtilis ATCC 605, 10 to 20 parts of bacillus subtilis ATCC 606 and 10 to 20 parts of bacillus subtilis ATCC 607. 5-10 parts of a seaweed extract; 3 to 8 parts of chitosan; 2-5 parts of humic acid; 1 to 3 parts of glycine betaine; 0.5 to 1.5 parts of a trace element compound; 30 to 40 parts of perlite powder; according to the present invention, the fertilizer has characteristics of significant inhibition effect on a variety of forest root pathogenic bacteria, wide prevention and control spectrum, and long-term and stable disease prevention effect by adjusting the rhizosphere micro-ecosystem. Diseases are prevented and controlled, and the growth and root development of the forest trees are remarkably promoted.

Methods are provided for enhancing the health, growth and / or sturdiness of turf grasses by applying a combination of microbes and / or their growth by-products to the turf grass roots and / or to soil. Specifically, in one embodiment, the subject invention utilizes Trichoderma harzianum and a strain of Bacillus amyloliquefaciens. The methods can further be used to improve maintenance and upkeep of natural turf grass fields used in, for example, athletics and recreation, as well as to increase the functionality and safety of these natural turf grass fields.

This invention discloses a microbial inoculant for controlling peanut root rot and its preparation method, belonging to the field of microbial materials technology. This invention screened and obtained a *Trichoderma harzianum* strain with highly efficient inhibitory effects against the pathogen causing peanut root rot. Firstly, the strain itself exhibits antagonistic activity against the peanut pathogen. Secondly, it was prepared as a spore suspension and fermented in solid-state with rice husks and wheat bran. During the solid-state fermentation process, the metabolites produced by *Trichoderma harzianum* promote the growth and development of plant roots, increasing plant biomass and yield. This microbial inoculant effectively controls peanut root rot, exhibiting good inhibitory effects against the pathogen, thus improving peanut quality and disease resistance. Furthermore, it improves soil ecological activity to a certain extent, making it worthy of application and promotion.

The present invention discloses a preparation for promoting strontium absorption in blueberries, belonging to the technical field of blueberry cultivation. The preparation consists of citric acid, potassium fulvate and Trichoderma harzianum. On the one hand, the preparation promotes the biological availability of strontium in strontium-rich soil, and on the other hand, by improving soil air permeability, it promotes the growth of blueberry roots and enhances the strontium absorption capacity of blueberry roots, thereby increasing the strontium content in blueberry fruits. The preparation for promoting strontium absorption in blueberries provided by the present invention has a simple formula, a simple process, and the raw materials used are all common raw materials, with a low cost. Applying the preparation for promoting strontium absorption in blueberries provided by this application can not only increase the strontium content in blueberry fruits, but also increase the yield of blueberries, which is of great significance for blueberry cultivation.

The invention discloses a special bacterial fertilizer for sorghum and application of the special bacterial fertilizer in prevention and control of sorghum diseases, and belongs to the technical field of microbial fertilizers and agriculture. The special bacterial fertilizer for sorghum comprises the following components in parts by weight: 8-10 parts of a composite microbial agent, 40-50 parts of sorghum residues, 5-10 parts of bentonite, 1-2 parts of potassium humate, 1-2 parts of composite trace elements, 10-20 parts of sawdust and 10-20 parts of rice bran, wherein the complex microbial inoculant consists of a bacillus subtilis microbial inoculant, a rhizobium microbial inoculant, a bacillus velezensis microbial inoculant and a trichoderma harzianum microbial inoculant. The special bacterial fertilizer for sorghum provided by the invention contains rich microorganisms and nutrient substances, and can effectively prevent and control sorghum diseases and enhance the disease resistance of sorghum. The components such as microorganisms and organic acids in the special bacterial fertilizer for sorghum can promote the formation of soil organic matters, improve the soil structure, improve the soil quality and effectively improve the growth environment of sorghum.

The invention discloses an African trichoderma harzianum strain 6-5 and application thereof, the disclosed strain is named as Trichoderma harzianum, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No. 41930. The bacterial strain has obvious bacteriostatic activity on crop pathogenic bacteria such as colletotrichum capsici, apple cyst, botrytis cinerea, alternaria longibrachiata and phytophthora capsici, and fermentation liquor of the bacterial strain has good prevention and control effects on apple anthracnose leaf blight, apple bitter rot and apple tree canker.

The invention provides a microbial complex microbial inoculant and application thereof in prevention and treatment of plant diseases, the microbial complex microbial inoculant comprises the following strains by mass: 30%-50% of bacillus subtilis; 20%-40% of bacillus amyloliquefaciens; 10%-30% of pseudomonas fluorescens; 5%-15% of trichoderma harzianum; and 2%-5% of an auxiliary agent. According to the microbial complex microbial inoculant disclosed by the invention, a multiple prevention and control mechanism of'bacteriostasis-competition-parasitism 'is constructed through the synergistic effect of various strains, and meanwhile, the double-layer effect of thallus protection and function enhancement is realized through the assistance of the auxiliaries, so that the prevention and control effect of the prepared microbial complex microbial inoculant is greatly improved.

The invention belongs to the technical field of coastal saline-alkali soil remediation, and particularly relates to a composite microbial agent for coastal saline-alkali soil remediation. The microbial agent is in a granule form, and comprises a carrier, which is a mixture of straw powder and duck manure; the active flora comprises bacillus licheniformis, bacillus megaterium, brevibacillus laterosporus, trichoderma harzianum spore powder, paenibacillus polymyxa, halomonas and pseudomonas fluorescens, the active flora is adsorbed in the carrier, and the halomonas and the pseudomonas fluorescens are subjected to embedding treatment; and the spraying film is sprayed on the surface of the carrier. Through the synergistic effect of multiple strains, the improvement effect on saline-alkali soil and the growth promoting effect on crop growth are improved, and the defect that the function of a single strain is limited is overcome.

The invention relates to the technical field of microbial fermentation feeds, in particular to a method for producing a protein feed through solid-state fermentation of distiller's grains based on Trichoderma harzianum pretreatment. According to the method, the vinasse is pretreated by the crude enzyme prepared from trichoderma harzianum, and the formula and fermentation conditions of an enzyme production culture medium are optimized, so that the filter paper enzyme activity of cellulase is remarkably improved. By adding the surfactant into a fermentation system and optimizing the variety of the surfactant, the yield of the fermented monosaccharide is improved, and the problem of musty taste in a traditional fermented product is solved while the degradation efficiency of lignocellulose is improved. Furthermore, a synchronous saccharification and fermentation process is adopted, vinasse is fermented through compounded saccharomycetes and crude enzyme liquid, the trichoderma harzianum fermented protein feed is prepared and obtained, digestion and absorption of animals are easier, lactic acid fermentation is conducted on the trichoderma harzianum fermented protein feed again through lactobacillus fermentum, the flavor of the feed is improved, the bitter taste of the feed is reduced, and the sour taste is improved. The method realizes efficient utilization of vinasse resources.

The invention relates to a soil remediation complex microbial inoculant composition and a preparation method thereof, and belongs to the technical field of soil remediation. Comprising the following raw materials: 10-15 parts of a microbial complex inoculant, 40-50 parts of a porous carrier, 20-25 parts of an organic matter and 3-5 parts of a surface protective agent, wherein the porous carrier is obtained by treating diatomite with phytic acid and polylysine. Paenibacillus polymyxa, trichoderma harzianum, bacillus subtilis and azotobacter chroococcum are taken as a microbial compound inoculant, modified diatomite is taken as a porous carrier, and the microbial compound inoculant is mixed with organic matters under the action of a protective agent, so that the soil remediation compound inoculant composition which can effectively improve soil and is excellent in strain activity is obtained.

The invention belongs to the field of microorganisms, and particularly relates to a banana endophyte compound fungicide as well as a preparation method and application thereof. According to the invention, a novel endophytic bacillus velezensis JDB15 strain and an endophytic trichoderma harzianum JDL4 strain are separated from a banana plant. The trichoderma harzianum JDL4 can inhibit botrytis cinerea, fusarium graminearum, rhizoctonia solani, peach anthracnose pathogen, peach brown rot pathogen and alternaria alternata, and has a good broad-spectrum antibacterial effect. Meanwhile, after the filtrate of the fermentation liquor of the trichoderma harzianum JDL4 and the bacillus velezensis JDB15 are compounded, the antibacterial effect is remarkably improved, and the control effect on banana fusarium wilt, pyricularia oryzae, tomato fusarium wilt and corn bipolaris maydis is also remarkably improved. The compound bacterial agent has wide biocontrol bacterial activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other plant diseases.

The invention discloses dsRNA of a targeted BdSpt7 gene, a biocontrol agent and application of the dsRNA. The sequence of the dsRNA of the targeted BdSpt7 gene is as shown in SEQ ID No.2. The invention further discloses a preparation method of the biocontrol agent of the targeted BdSpt7 gene. The active ingredient of the biocontrol agent is the dsRNA of the target BdSpt7 gene. The invention also provides an application of the botryosphaeria dothidea BdSpt7 gene in prevention and treatment of apple botryosphaeria dothidea, and a nucleotide sequence of the botryosphaeria dothidea BdSpt7 gene is shown as SEQ ID No.1. The invention also provides an African trichoderma harzianum engineering strain for expressing dsRNA, the African trichoderma harzianum engineering strain is integrated with a dsRNA expression box for interfering gene expression of a target gene BdSpt7, the dsRNA expression box is shown as SEQ ID No.1, and the African trichoderma harzianum is an African trichoderma harzianum with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 41709. The dsRNA targeting the BdSpt7 gene can significantly reduce the pathogenicity of the physalospora piricola through interference, and the African trichoderma harzianum engineering strain can colonize on the plant surface and continuously express the dsRNA so as to play a role in preventing and treating the physalospora piricola, and is green and environment-friendly.

A functional microbial organic fertilizer for improving saline-alkali land, and its preparation method and application, belong to the field of fertilizer technology. In order to solve the technical problem that the high salt ion content in saline-alkali soil has multiple adverse effects on plant growth, the present invention provides a functional microbial organic fertilizer for improving saline-alkali land, which is prepared by a functional microbial agent prepared from Bacillus amyloliquefaciens, Copperbacterium insecticide, Trichoderma harzianum, Halobacterium gesneri and Bacillus halogenide, and a functional microbial carrier organic fertilizer added with furfural residue and citric acid residue. The functional microbial organic fertilizer provided has the functions of increasing the abundance of saline-alkali soil microorganisms, improving soil physical and chemical properties, regulating soil pH, increasing the proportion of soil aggregates, and increasing the content of available phosphorus and available potassium. The functional microbial organic fertilizer provided by the present invention can be used to effectively improve saline-alkali soil.

The present invention belongs to the field of Trichoderma secondary metabolites, and discloses a natural product derived from Trichoderma harzianum, a preparation method and an application thereof. The natural product has a structure shown in Formula I. The present invention separates and purifies the Trichoderma harzianum secondary metabolites to obtain the Trichoderma harzianum source natural product LU-11, which can be used to inhibit the growth of Fusarium graminearum PH-1, a wheat head blight disease, and induce and improve the disease resistance of wheat, providing a certain theoretical basis for the development of biological pesticides.

The invention belongs to the technical field of agricultural microbial control, and particularly relates to a growth-promoting and disease-preventing compound microbial agent and a preparation method and application thereof, and the compound microbial agent specifically comprises microbial powder, a growth auxiliary material and a drying agent; the microorganisms comprise vermiform yellow, trichoderma harzianum, bacillus velezensis, bacillus licheniformis and brevibacillus laterosporus. According to the compound microbial agent, through the synergistic effect of multiple strains, the control effect of the compound microbial agent on verticillium dahliae is enhanced, and the defects that a single biocontrol bacterium is single in biocontrol mechanism, poor in environment adaptability, incapable of being colonized in soil for a long time and unstable in control effect are overcome; the compound microbial agent can increase the yield of cotton and pepper and improve the quality of cotton fibers, and provides scientific and technological support and decision service for realizing green, environment-friendly, cost-saving and efficiency-increasing modern agriculture.

The invention belongs to the technical field of microorganisms, and particularly discloses trichoderma harzianum NBU-1 and application thereof. The Trichoderma harzianum NBU-1 is preserved in the Guangdong Microbial Culture Collection Center on March 26, 2025, the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCCNO: 66053. The invention discloses trichoderma harzianum NBU-1 and application thereof, the trichoderma harzianum NBU-1 is applied to prevention and treatment of wheat sharp eyespot, and the activity of rhizoctonia cerealis R0301 and the growth of scab are effectively inhibited.

The invention discloses a microbial agent for promoting sand fixation in sand land and a preparation method and application thereof, and belongs to the technical field of microorganisms. The microbial agent comprises bacillus megatherium, pseudomonas fluorescens, lactobacillus mucus, trichoderma harzianum and azotobacter chroococcum which are mixed in the form of bacterial liquid, the volume ratio of the bacterial liquid is (3-5): (2-3): (2-3): (1-2): (1-2), and all bacterial strains have a synergistic effect. When the microbial agent is applied to soil, the soil stability is obviously improved after the microbial agent is applied, the water retention rate of sand land soil can be improved by 15.9 times within 10 days, the sand rising wind speed reaches 18.5 m / s and above, and the vegetation coverage can reach 32% and above after one year. The microbial agent disclosed by the invention is simple and convenient in preparation method, can remarkably promote sand fixation in the sand land and improve the survival rate of plants in the sand land, has the characteristics of environmental protection and high efficiency, and is easy to popularize and apply.

The invention discloses a trans-boundary synthetic flora (SMC) for preventing and treating wheat stem rot as well as preparation and application of the trans-boundary synthetic flora. The synthetic bacterial flora is prepared by compounding Trichoderma harzianum T19 with the preservation number of CCTCC (China Center for Type Culture Collection) No: M 20251896, bacillus subtilis BS-Z15 with the preservation number of CCTCC No: M 20251892, two strains of growth promoting bacillus PM9 and PM16, and two strains of auxiliary bacillus S8 and S32 according to a specific proportion. Compared with an existing single antagonistic bacterial agent, the synthetic flora disclosed by the invention has the advantages that the bacteriostasis rate of the synthetic flora on Fusarium pseudograminearum under a non-sterile soil condition can reach 70% or above, and the disease index is reduced by 70.01%; meanwhile, the plant height of wheat is increased by 28.63%, the root length is increased by 24.53%, the fresh weight is increased by 181.21%, the dry weight is increased by 184.64%, the thousand seed weight is increased by 10.39%, and the rhizosphere microbial community structure and the soil enzyme activity are remarkably improved. The method can be used for green prevention and control and yield increase of basal stem rot of wheat and other gramineous crops.

The invention discloses a Trichoderma harzianum-containing biocontrol fertilizer and a preparation method thereof, and belongs to the technical field of mixed fertilizers, the fertilizer comprises composite matrix particles (containing an organic carrier, an inorganic fertilizer and trace elements), a microsphere coating layer (composed of sodium alginate / chitosan gel microspheres) coated with Trichoderma harzianum on the surface, and a water-soluble fertilizer coating layer (composed of sodium alginate / chitosan gel microspheres) coated with Trichoderma harzianum on the surface, and the outermost layer is a biodegradable protective film (modified starch / PVA / PLA). The preparation method comprises the following steps: granulating a matrix, preparing a bacterial suspension, forming bacteria-containing gel microspheres (the spore recovery rate is greater than or equal to 95%), attaching the microspheres to the matrix, and coating the outer layer. According to the structure, the survival rate of viable bacteria stored at room temperature for 120 days is larger than or equal to 68.3%, the rhizosphere bacterial count is larger than or equal to 4.58 * 10 < 6 > CFU / g soil after the viable bacteria are applied to the soil for 30 days, soil-borne diseases are remarkably inhibited, and the crop yield is increased by 13.5% (cucumber yield is increased by 13.5%).

The invention provides a compound microbial agent capable of resisting diseases and insect pests and a preparation method thereof, and the compound microbial agent consists of the following substances in parts by mass: 50-100 parts of bacillus subtilis, 40-80 parts of beauveria bassiana, 35-75 parts of trichoderma harzianum, 20-50 parts of modified straw powder, 10-15 parts of a humic acid-diatomite complex, 10-15 parts of trehalose, 5-10 parts of chitin powder and 5-10 parts of shellac. According to the invention, the modified straw powder and the humic acid-diatomite complex are adopted as a core carrier, so that the defects of poor adsorbability and nutrition deficiency of the traditional diatomite or synthetic carrier are overcome, and a high-porosity and nutrient-rich living environment is provided for bacillus subtilis, beauveria bassiana and trichoderma harzianum; compared with the prior art, the method provided by the invention has the advantages that the cost is low, the field planting rate and the field survival rate of viable bacteria in the microbial inoculum are remarkably improved, meanwhile, controllable coating is performed by utilizing an ethanol solution of shellac, a refractory synthetic polymer coating material is replaced, thalli are protected from environmental stress, and meanwhile, the risk of lasting residual pollution of a chemical coating material in soil is avoided.

The invention relates to the technical field of cyanobacterial bloom control in water, and particularly discloses a compound enzyme-bacterium synergistic preparation for cyanobacterial bloom control, a preparation method and a use method. The preparation comprises the following components in percentage by mass: compound enzymes (10-20% of cellulase, 8-12% of beta-1, 3-glucanase and 3-8% of lipase), functional bacteria (10-20% of bacillus subtilis, 8-15% of trichoderma harzianum and 5-10% of bacillus amyloliquefaciens), an enzyme stabilizer (3-8% of trehalose), an adsorption carrier (15-25% of Fe < 3 + > modified charcoal and 8-15% of diatomite) and auxiliary components. The preparation method comprises the steps of carrier modification, solid fermentation and microcapsule embedding. During use, 3-20kg / 1000m < 3 > of water is added for the first time, 2-10kg / 1000m < 3 > of water is added every 7-14 days in the maintenance stage, and synchronous aeration is performed. Through the synergistic effect of beta-1, 3-glucanase and trichoderma harzianum, the algicidal rate is 92%, and the spore germination inhibition rate is 82%; the Fe < 3 + > modified charcoal improves the stability of the enzyme in an alkaline environment (80% of activity is retained when the pH value is 9); the method is suitable for eutrophic water with chlorophyll a greater than or equal to 30 [mu] g / L, and has no chemical residue risk.

The invention relates to a preparation method of a plant disease control fungicide, which comprises the following steps: taking traditional Chinese medicines with an antibacterial effect as raw materials, and carrying out first-step fermentation by adopting Trichoderma harzianum; beauveria bassiana and Penicillium baylii are subjected to second-step fermentation, bacillus velezensis is subjected to third-step fermentation, fermentation liquor is collected, a filter cake is further subjected to reflux extraction, an extracting solution and the fermentation liquor are collected, combined and concentrated into clear paste, an ethanol solution is added, standing is carried out, supernate is taken, borneol, gelatin and cellulose acetate are added to prepare mixed liquor, ultrafiltration is carried out, ultrafiltrate is collected, and freeze-dried powder is obtained. The microbial inoculum required by the invention is obtained. According to the invention, specific traditional Chinese medicines are adopted, an excellent broad-spectrum antibacterial system is formed through specific microbial fermentation, and the antibacterial system is compounded with borneol, gelatin, cellulose acetate and other components to form the fungicide, so that the effectiveness duration and the effect taking rate of the fungicide on the surface of a plant are effectively improved, and efficient prevention and treatment on various diseases such as fungi, bacteria and the like are formed.

The invention provides a pill-type organic fertilizer special for vegetables. The pill-type organic fertilizer is prepared from the following raw materials in parts by weight: modified biochar, fermented livestock and poultry manure, plant source protein zymolyte, a complex microbial inoculant, a trace element supplement and a binder, and a method therefor; the complex microbial inoculants comprise actinomycetes, bacillus amyloliquefaciens and trichoderma harzianum; the trace element supplement comprises EDTA (Ethylene Diamine Tetraacetic Acid) chelated zinc, EDTA chelated iron and boric acid. Therefore, on one hand, the organic fertilizer can effectively improve the facility agriculture soil environment, adjust the soil acidity and alkalinity, reduce the salinization degree, promote balance and stability of microbial communities and restore and improve the ecological function of soil; on the other hand, the utilization rate of the fertilizer can be greatly improved, and the plant-source proteolysis provides a quick-acting nitrogen source to promote root development; the microelement supplement meets the requirements of vegetables on microelements; nutrients are balanced, vegetable stress resistance is enhanced, and photosynthetic efficiency is improved; and the binder enables the fertilizer to form uniform particles, is suitable for mechanical fertilization, and improves the fertilization efficiency.

The invention belongs to the technical field of soil improvement, and discloses a preparation method of a composite microbial agent and application of the composite microbial agent in continuous cropping stress resistance, the active ingredients of the composite microbial agent comprise Gliocladium mucilaginosum, Trichoderma harzianum and Pseudomonas chlororaphis, and a carrier contains a basic carrier, a synergistic component and a soil adaptive component (oyster shell powder or wormcast particles). The preparation method comprises the following steps: strain fermentation, carrier construction, solid-state co-fermentation, and mixing of a dried fermentation product and a carrier. After the compound microbial agent is used, the abundance of gliocladium mucilaginosum, trichoderma harzianum and pseudomonas chlororaphis in continuous cropping crop soil is obviously increased, and the abundance of fusarium oxysporum is obviously reduced; the acidification condition of the acidified soil is obviously improved, the content of organic acids (ferulic acid and vanillic acid) in the soil is obviously reduced, and the soil becomes loose from hardening, so that the root activity is improved, and the plant diseases and insect pests are resisted; the single fruit weight and sugar degree can be remarkably improved, the fruit storage rotting rate is reduced, and the effect is remarkable.

The invention discloses a modifier for reducing wine grape soil obstacles and a preparation method and application thereof, and belongs to the technical field of soil improvement. According to the invention, the specific complex microbial inoculant composed of bacillus subtilis, bacillus licheniformis and trichoderma harzianum is adopted to ferment the grape pomace, antibacterial substances and organic acids can be generated, and saline-alkaline obstacles of soil can be relieved; the fermentation product contains a large amount of bacillus subtilis, bacillus licheniformis and trichoderma harzianum, and can occupy the ecological niche of the root system through competition, optimize the rhizosphere environment and improve the biological enzyme activity of soil. Through the synergistic effect with the decomposed straw, the humic acid and the furfural residues, the rhizosphere microenvironment can be remarkably improved, decomposition and nutrient conversion of organic substances in soil are accelerated, the soil fertility is improved, root systems can better extend and absorb nutrients, plant growth is promoted, and the yield and quality of fruits are improved.

The invention discloses a preparation method and application of an anti-continuous cropping microbial fertilizer, and aims to solve the problems of aggravation of crop diseases and insect pests and reduction of yield and quality caused by continuous cropping planting. The preparation method comprises the following steps: activating and uniformly mixing bacillus subtilis and bacillus licheniformis, and adding an alpha-lactalbumin solution and a calcium chloride solution to prepare a bacillus aggregate; adding bentonite into the polytrehalose solution, and performing ultrasonic treatment on the mixture and the humic acid solution to prepare a modified polytrehalose-bentonite compound; uniformly mixing acremonium terricola, trichoderma harzianum and a bacillus aggregate, and adding a wall material solution to prepare a compound bacterium microcapsule preparation; and finally, mixing the microcapsules with the corn straw biochar, the chitosan oligosaccharide and the like to obtain a finished product. Through calcium ion induction and humic acid modified embedding, the strain stability and viable count are improved, soil organic matter and nitrogen phosphorus and potassium nutrients can be activated, pathogenic bacteria are inhibited, the crop rhizosphere environment is improved, the growth index and quality of red kidney beans and potatoes are effectively improved, and the method is suitable for continuous cropping resistant planting of crops.

The invention provides a trichoderma fungicide for promoting growth of allium plants and application of the trichoderma fungicide, the trichoderma fungicide is a trichoderma harzianum strain MCBC3, the trichoderma fungicide and diammonium phosphate are applied in a combined mode, the application amount of the diammonium phosphate is 15 kg / mu, and the application amount of the trichoderma fungicide is 1 L / mu; existing trichoderma resource development focuses on a normal soil environment, the trichoderma harzianum MCBC3 is screened out from the saline-alkali soil for the first time, the salt resistance and the alkali resistance of the trichoderma harzianum MCBC3 directly aim at double stress of high salt and high alkali of the saline-alkali soil, and the technical bottlenecks that a traditional strain is poor in adaptability and low in survival rate in an extreme environment are solved.

The invention discloses a method for carrying out soil treatment by using ardealite and a microbial agent, and aims to solve the problem that ardealite as an industrial by-product is difficult to recycle. According to the method, 5% of a microbial agent (one of trichoderma harzianum, arbuscular mycorrhizal fungi or bacillus laterosporus) and 5%-15% of biochar are applied to phosphogypsum in a combined mode, the nutrient content of a phosphogypsum matrix is remarkably increased, and meanwhile specific pollutants (such as soluble phosphorus, fluorine and heavy metal) are stabilized and solidified. Experimental results show that the technology can effectively regulate and control the pH value of the phosphogypsum matrix, obviously increase the diversity of effective microbial communities and improve the micro-ecological environment of the phosphogypsum matrix. The ardealite combined with the microbial agent and the biochar can be used as a plant growth matrix or a soil substitute material for ecological restoration of mines, has the characteristics of low cost and high efficiency, and provides a feasible way for harmlessness and soil utilization of the ardealite.

The invention discloses a trichoderma complex microbial inoculant capable of promoting wheat growth and preventing and treating wheat stem rot and application of the trichoderma complex microbial inoculant. The trichoderma complex microbial inoculant comprises a trichoderma asperellum strain XX-57 and a trichoderma harzianum strain PY-127, and the preservation number of the trichoderma asperellum strain XX-57 is CGMCC No. 41937; the preservation number of the trichoderma harzianum strain PY-127 is CGMCC (China General Microbiological Culture Collection Center) NO. 41938. The invention further specifically discloses application of the trichoderma complex microbial inoculant in promoting wheat seed germination and wheat growth and preventing and treating wheat basal stem rot, the trichoderma complex microbial inoculant is applied 10-20 days after wheat seedling emergence, and it is found that compared with single trichoderma inoculation, the trichoderma complex microbial inoculant has the promoting effect on wheat plant height, root length, SPAD value, fresh weight and dry weight; the control effect on wheat stem rot is better, and particularly the control effect is more obvious when pathogenic bacteria are inoculated in advance.