651 results about "Trichoderma harzianum" patented technology

The invention discloses a functional microbial fertilizer and a production method thereof. The production method comprises the following steps: grinding 60%-70% of mushroom residue and 15%-25% of livestock manure to 2-3mm to obtain the major material by a grinder, regulating water content to be 60%-65%; adding composite fermenting agent consisting of bacillus subtilis, bacillus licheniformis, trichoderma harzianum and trichoderma viride in accordance with 50g-100g / T; stacking, fermenting and maturing for 15-30d, and turning 5-10 times during the process; evenly mixing the major material prepared from fermentation with auxiliary materials consisting of 6%-10% humic acid and 6%-10% rice hull; adding arbuscular mycorrhiza agent in accordance with 50g-100g / T; and sieving with a 3mm sieve to obtain the fertilizer. The invention provides an economic and effective technological approach for the resource recycling of agricultural wastes in China, and has important application prospect on promoting the development of green agriculture, controlling and reducing rural environmental pollution, improving rural eco-environment and other aspects.

The invention relates to a bio-protein feed prepared by adopting a cellulose raw material, and the bio-protein feed provided by the invention comprises an organic material and complex bacteria, wherein the weight of the complex bacteria is 1-10% of that of the organic material; and the organic material comprises the cellulose raw material, rapeseed meal and bran; the cellulose raw material is one or more of corn stalks, corn cobs, straw, manioc waste and fruit residue; and the complex bacteria comprises bacillus subtilis, microzyme, lactobacillus and cellulose decomposition bacteria, and the cellulose decomposition bacteria is one or more of trichoderma harzianum and white-rot fungi. In the bio-protein feed, the content of probiotics is more than 108cfu / g, the bio-protein feed contains a variety of prolease, cellulase, amylase and the like, the preparation process is simple, and the fermentation period is short. The invention further discloses a preparation method of the feed.

The invention relates to a trichoderma harzianum composite bacteria culture and application of the same in the aspect of plant protection, and belongs to the technical field of biology. The strain of the trichoderma harzianum composite bacteria culture is preserved in the China General Microbiological Culture Collection Center (CGMCC), and classified and named as Trichoderma harzianum MDCGTH 18, with a preservation number of CGMCC NO.2965. The Trichoderma harzianum MDCGTH 18 composite bacteria culture utilizes antagonistic microbes for biological prevention and treatment, simultaneously has the functions of inducing the disease resistance of plants and increasing the yield, has obvious effect of controlling aspen root rot, apple root rot, citrus yellow shoot, watermelon fusarium wilt, tobacco bacterial wilt, wheat scab, full rot, root rot, banded sclerotial blight, cucumber powdery mildew, downy mildew, vegetable gray mold, fusarium wilt and various other epidemic diseases, can be independently taken as a biological control microbial inoculum for plant diseases, and can also be mixed with other biological antiseptics, plant antibiotics, plant growth regulators and the prior antiseptics to prepare various biological antiseptics for controlling different plant diseases.

The invention relates to a biotechnology dealing agricultural discard stem with an highly effective stem decomposing composite bacterial, which dissolves the problem of single species of applying microbiological bacterial, weak breaking up, low total degradability rate of cellulose, long developmental cycle. The composite bacterial comprises the following parts: yellow archespore wool bacterium leather bacterial, mushroom bacterial, haci trichoderma, green trichoderma, coninic trichoderma, aspergillus niger, bacillus subtilis, feed bacillus, bacillus megaterium, candida, distillery yeast, food-yeast, brown sphere azotobacter, thermoactinomyces, and so on. The composite bacterial can overall degrade lignin, cellulose, hemicellulose and other organic material in the stem, has a more than 80% degrading rate, a 10-15 days short cycle, keeps a long time for providing a solid bacteria.

The invention provides a special bacterial manure for peanut planting, which comprises the following components in parts by weight: 5-25 parts of a compound microbial agent, 30-65 parts of dreg composite powder, 5-20 parts of starch and 10-25 parts of bentonite, wherein the compound microbial agent is composed of bacillus subtilis agent, a rhodopseudomonas palustris agent, a trichoderma harzianum agent and a paecilomyces lilacinus agent; the mass ratio of the bacillus subtilis agent to the rhodopseudomonas palustris agent to the trichoderma harzianum agent to the paecilomyces lilacinus agent is 5:2:1:2, and the total effective viable count of the compound microbial agent is10 billion cfu / g. The bacterial manure is mainly used as a base fertilizer, and can change the microenvironment around the roots of peanuts to prevent various plant diseases and insect pests while promoting nutrient absorption of the peanuts, so that the yield and quality of the peanuts are improved.

The invention discloses a microbial fertilizer producing by using agricultural wastes and preparation thereof. Main materials are obtained by mixing and smashing 20%-40% oil sally bean dregs, 40%-60% crop straw and 15%-25% poultry excrement, moisture content achieves 60-65%, composite leaven is added according to 150g-200g / T to pile to be cone-shaped, yellow mud is used on the outside for sealing, a plastic film is used for covering in a sealing mode, after stacking, fermentation and digesting, and the composite leaven is composed of bacillus subtilis, bacillus licheniformis, trichoderma harzianum and trichoderma viride according to the mass ratio (W / W) of 1.5:1:1:2.5:4. Auxiliary materials made of 5%-10% mushroom dregs and 6%-10% rice husk ash are evenly mixed with fermented and prepared main materials, arbuscular mycorrhizal fungi agent are added according to 600-1000g / T for compounding, and the microbial fertilizer is prepared after passing a 3mm sieve. Good production and efficiency improvement is obtained through application, and the microbial fertilizer has good application value.

Described herein are compositions and methods related to isolated Trichoderma harzianum and strains thereof.

The invention provides a novel microbial fertilizer special for tobaccos. The fertilizer comprises the following components in parts by weight: 5 to 25 parts of a complex microbial inoculant and 75 to 95 parts of a carrier, wherein the complex microbial inoculant contains 1.6 to 8 hundred million cfu / g of bacillus subtilis, 1.6 to 8 hundred million cfu / g of bacillus licheniformis, 0.4 to 2.0 hundred million cfu / g of trichoderma harzianum and 0.4 to 2.0 hundred million cfu / g of calicheamicin streptomyces hygroscopicus; the carrier is a powdered material which are made by performing microbe fermentation and decomposition on rape seed cakes, bean pulp cakes and sesame-seed cakes, and then performing drying and sieveing. The microbial fertilizer contains bacterial strains for antagonizing fungus pathogenic bacteria, bacterial strains for preventing and curing the breeding of soil pathogenic bacteria, bacterial strains for improving the soil environment and the like. The carrier is fermented three-cake fertilizer. The invention also provides a preparing method for the microbial fertilizer. The microbial fertilizer special for the tobaccos, provided by the invention, is powder, is capable of absorbing the water and preserving the moisture. The fertilizer is mainly used as the base fertilizer when fertilizing, the soil fertility can be improved, the nutrient absorption of crops is improved, the diseases of a plurality of crops can be prevented, and the quality and the yield of tobacco leaves can be effectively improved.

The invention discloses a trichoderma preparation for preventing and treating soil-borne diseases of crops and a preparation method thereof. The compositions of the preparation comprise that: a carrier comprises a useful quantity of Trichoderma harzianum strains Th-192 CGMCC NO.2634. The preparation method comprises the steps of propagation of the strains Th-192, liquid and solid fermentation culture, addition of an accelerant, and the like. The preparation has the advantages of wide sources of raw materials, short production period, low cost, large spore yield, convenient use, wide applicable crops and obvious functions of promoting growth and increasing production.

The invention provides a phosphate-solubilizing composite microorganism fungicide, which has effective components of pantoea ananatis, pythium oligandrum, trichoderma harzianum and paecilomyceslilacinus. According to the invention, high-efficiency phosphate-solubilizing bacterium-increase bacterium pantoea ananatis with wide applications and fungus of high-efficiency antagonistic crop disease areorganically combined, by applying the composite fungicide of the invention, while soil phosphorin is efficiently utilized and phosphatic fertilizer utilization rate is improved, the effect of antagonistic microorganisms in preventing soil-borne diseases can be sufficiently played, and the effects of solubilizing phosphate to increase yield and resisting diseases to increase yield are achieved. The phosphate-solubilizing composite microorganism fungicide has tremendous application value.

A strain of Trichoderma harzianum is obtained that is useful as a nematode inhibitor, fungicide and plant growth promoter. The strain has ATCC accession number PTA-3701. The strain is isolated by treating Trichoderma harzianum isolated from experimental fields of Central Institute of Medicinal and Aromatic Plants (CIMAP) Field Station with a mutagen such as ethyl methyl sulphonate, and isolating a whitish and fast growing strain of Trichoderma harzianum.

The invention relates to Trichoderma harzianum Rifai WRF-2 and use thereof. The bacterium strain was preserved in China General Microbiological Culture Collection Center on January 21st, 2009, with a preservation number of CGMCC No.2870. The bacterium belongs to deuteromycetes and is a lower fungus that is short in culture period and quick in growing and propagating speed and produces a large amount of uniform culture liquid for lignin degrading enzyme-generating bacteria in a short time compared with a major part of basidiomycetes to which whiterot fungi belong. The invention also provides a method for culturing and producing lignin peroxydase (Lip) and manganese peroxidase (MnP) by the fermentation of the bacterial strain in a liquid enzyme-generating culture medium, which can produce high-acitivity LiP and MnP in a short time, and is simple in fermentation process, stable, low in cost, and high in yield.

The invention relates to a compound microbial fertilizer and a preparation method thereof. The compound microbial fertilizer is characterized by being prepared from the following materials in percentage by weight: 40.0-50.0 % of rape seed cakes, 10.0-20.0% of crops straws, 1.5-3.0% of mycothallus, 0.5-1.0% of bacterium thallus and 35.0-40.0% of water. By using the solid fermentation method twice, the invention organically combines the fungi (Trichoderma harzianum and Aspergillus aculeatus for biological control effect) and the bacteria (Bacillus subtillis and Bacillus megatherium for growth-promoting effect), and realizes a simple preparation method. The compound microbial fertilizer has the advantages of environmental condition resistance, high count of active viable bacteria, less influence by the environment after application, slow release of thallus and long-term effective colonization at rhizosphere of plants, promotes plant growth and simultaneously has a certain biological control effect on soil-borne diseases.

The invention relates to a high-efficiency Trichoderma harzianum strain for controlling plant fungus diseases and promoting plant growth. The Trichoderma harzianum strain has been collected at the China General Microbiological Culture Collection Center, and the collection number is CGMCC No.7230. The Trichoderma harzianum strain provided by the invention has the advantages of high capacity for inhibiting plant pathogenic fungus growth, no environment pollution and high ecological safety, can be widely used for controlling plant pathogenic fungi, especially Pythium spp., Fusarium spp. and Botrytis cinerea, and has high application value in biological control on melons, fruits, vegetables and crops. The high-efficiency Trichoderma harzianum strain can be used independently, or used together with other organic and inorganic nutrients required by plant growth; and the prepared biological compound fertilizer has obvious effects on promoting growth, inhibiting premature senility, preventing diseases, increasing yield and improving quality.

The invention provides a composite microbial inoculant for controlling plant continuous-cropping diseases, which comprises trichoderma harzianum, bacillus subtilis, and gliocladium roseum, and also comprises phosphate-solubilizing bacteria and potassium-solubilizing bacteria; the accession number of the trichoderma harzianum strain is CGMCC NO. 7861; the accession number of the bacillus subtilis strain is CGMCC NO. 7850; the accession number of the gliocladium roseum strain is CGMCC NO. 9008. The invention also provides a preparation method and an application of the composite microbial inoculant. Through the selection of high-efficient bacterial strains such as disease-preventing trichoderma, bacillus subtilis, gliocladium roseum, phosphate-solubilizing bacteria and potassium-solubilizing bacteria, the composite microbial inoculant of the invention promotes the growth of crops, improves the utilization rate of fertilizers, enhances the stress resistance such as disease resistance and the like, improves the quality of crops, and can control diseases such as root rot, verticillium wilt, damping off, botrytis, epidemic diseases, powdery mildew, sclerotinia rot, seedling-stage damping off, and the like.

The invention belongs to the technical field of microorganism, in particular to a compound microbial preparation used for directly decomposing crop straws, namely an efficient straw-decomposing inoculant. The efficient straw-decomposing inoculant comprises the following raw materials by weight percentage: 20-30 percent of bacillus subtilis, 10-15 percent of white light gray streptomycete, 5-10 percent of endomycopsis fibuligera, 5-10 percent of candida utilis, 10-15 percent of aspergillus, 10-15 percent of paecilomyces lilacinus, 10-15 percent of trichoderma harzianum and 20-30 percent of thermophilus streptomycete. The efficient straw-decomposing inoculant is organically combined with soil no-tillage, shifting and fallow to achieve the comprehensive purposes of protecting the environment, improving soil, increasing the utilization rate of fertilizers, increasing the quantity and the quality of agricultural products and developing the ecological agriculture.

The invention relates to a composite microbial inoculant and its preparation method, and overcomes the problems of slow decomposing agent start and incomplete decomposition in the prior art. The invention provides a straw decomposing agent for quick start of decomposition at low temperature, and the straw decomposing agent comprises a strain YO-H, bacillus subtilis, Saccharomyces cerevisiae, Aspergillus niger and Trichoderma harzianum. The straw decomposing agent provided by the invention has the advantages that: 1. it employs the strain YO-H, and can quickly start decomposition under the condition of a low environmental temperature; 2. the strain YO-H cooperates with other decomposing fungi to achieve a better decomposing effect; and 3. an additional enzyme preparation is added to improve the cellulose activity of the decomposing agent, about 3-4 days are left before mass propagation of decomposing fungi and generation of new enzyme, and during the period, the additionally added enzyme preparation is employed to degrade lignin and cellulose, that is to say the decomposition time is 3-4 days ahead of schedule.

The invention provides a microbial soil conditioner. The microbial soil conditioner comprises the following raw materials: an organism carrier, bacillus amyloliquefaciens with the concentration higher than or equal to 2 billion cfu / g, bacillus mucilaginosus with the concentration being 0.15-0.3 billion cfu / g, trichoderma harzianum with the concentration being 30-100 million cfu / g, paecilomyces lilacinus with the concentration being 10-50 million cfu / g and streptomyces griseus with the concentration being 10-50 million cfu / g. The invention further provides a preparation method of the microbial soil conditioner. The preparation method comprises an organism carrier preparation step and a finished product preparation step. The microbial soil conditioner can activate beneficial microbial flora of local soil, form a PGPR (plant growth promoting rhinoacteria) action with the beneficial microbial flora, enhance the activity of a plant root system, promote growth and effectively control pest and disease damage; the preparation method is simple and convenient to use.

The invention relates to a composite microbe fertilizer. The composite microbe fertilizer is prepared by 25-35 wt% of vegetable garden soil, 5-10 wt% of active functional bacteria, 5-15 wt% of mineral powder, 25-30 wt% of an organic fertilizer, 15-20 wt% of an inorganic composite fertilizer, and water; wherein the sum of the components is 100%. The active functional bacteria comprise lactic acid bacteria, bacillus subtilis, Bacillus megaterium, trichoderma, aspergillus niger, monascus, Trichoderma harzianum, aspergillus aculeatus and yeast, the mineral powder is a mixture of one or more of peat, zeolite, vermiculite, montmorillonite, calcium carbonate and expanded perlite, fineness of the mineral powder is less than or equal to 200 meshes, the organic fertilizer contains decomposed manure, a rapeseed cake fertilizer, and straw, and the weight ratio of the manure to the rapeseed cake fertilizer to straw is 5.5-7.5:2.5-3.5:0.5-1.5.

The invention relates to a preparation method of trichoderma multifunctional soil modifying agents. The preparation method comprises the steps that: trichoderma harzianum SH2303 with the CGMCC (China General Microbiological Culture Collection Center) NO. 4963 is cultured on a potato dextrose agar (PDA) culture medium for 2 to 3 days, a bacterial cake is punched by a hole puncher with the diameter being 5mm, the bacterial cake is inoculated into a seed culture medium for 5 to 7 days at the bacterium inoculation quantity of three tablets per bottle and is then inoculated into a spore production culture medium, the fermentation is carried out for 5 to 7 days under the optimized culture condition in a 300 L fermentation tank, and the number of thick-wall spores is (6 to 7)*10<8> / mL; and fermenting liquid and carriers are mixed according to the weight ratio of 1:1, the secondary open type solid fermentation is carried out for 5 to 7 days at the normal temperature, and the number of trichoderma conidiospores and chlamydospores is 200 to 500 million / g. The soil modifying agents prepared by the method have the main purposes of improving the primary and secondary salinization of vegetable planting soil and degrading the organic phosphorus pesticide residue soil, and have the effects of treating soil-borne diseases, promoting the crop growth and improving the crop yield. Because only one trichoderma strain is utilized, the fermentation process is easy to optimize, the microbial inoculum preparation cost is low, the commercial large-scale popularization is easy to realize, and high field general use values are reached.

The invention discloses a biological organic fertilizer containing compound bacteria and a preparation method. The method comprises the following steps: mixing a raw material containing an organic matter and plant ash to prepare a material to be fermented; dissolving cellulase and EM bacteria liquid in water to prepare a material fermentation spray liquid; uniformly spraying the fermentation spray liquid onto the material to be fermented, performing anaerobic fermentation for 6-7 days and aerobic fermentation for 15-20 days; then granulating the fermented and decomposed raw material, sprayingplant oil on the fertilizer particles in a coating machine, finally uniformly mixing Trichoderma harzianum chlamydospore powder, Trichoderma viride chlamydospore powder and a powder form bacterium powder carrier in a feed mixer and sending them in the coating machine, and coating the powder mixture above on the particles coated with plant oil. Compared with the prior art, the fertilizer has the advantages of thoroughly decomposing of the raw material, large hardness of the fertilizer particles, less impurity bacterium, high bacterium survival rate, comprehensive nutrition, and obvious diseases prevention and treatment effects to the crops as well as production and efficiency enhancement performance.

The invention relates to an environment-friendly prevention and control method of panax notoginseng diseases and belongs to the technical fields of plant disease prevention and control and plant cultivation. The prevention and control method comprises land selecting and soil preparation, panax notoginseng planting and seed handling and growing period management, wherein thoroughly-decomposed cow dung, begasses, compound fertilizers and plant ash are mixed according to the volume ratio of 5:4:1:1; 6000-10000kg / hm<2> of improved soil is adopted; xiandi, 1cm of marigold limbs and trichoderma harzianum are applied after ditching and less xiandi is used for mixing with roots before transplanting; crofton weed and the marigold limbs are covered on a moisture face after transplanting, bottom-ventilated row covers are constructed, two sides of the bottom-ventilated row covers are vertical to the ground, and the bottom-ventilated row covers are 15cm in length, 1.8m in width and 1.2m in arc height; the trichoderma harzianum and the marigold infusion are applied in a sprayed mode according to the ratio of 1:200 after emergence of seedling to irrigate roots, and 100g / L of jasmonic acid methyl ester are applied to leaf faces in a sprayed mode. The environment-friendly prevention and control method has the advantages of being simple in operation, low in cost and easy to popularize, protecting the environment, being environment-friendly and the like, lays a good foundation to pollution-free planting of the panax notoginseng and has good economic benefits, ecological benefits and social benefits.

The invention discloses a microbial agent, a preparation method thereof, and an application thereof, which relate to the technical field of microbial agent. The microbial agent includes Streptomyces albidofuscus CGMCC No.13717, Paecilomyces lilacinus CGMCC No. 13898, Streptomyces longus and mixed bacteria, wherein the mixed bacteria includes yeast, Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Brevibacillus laterosporus and Trichoderma harzianum. Through combination of bacteria-fungi and the synergistic effect of multi-strains, the microbial agent has the advantages ofhigh activity of strains, high efficiency and multi-functions in treating waste water, waste gas or waste. In addition, the invention also provides a preparation method of a microbial agent, which adopts a double-layer immobilization (preliminary immobilization and secondary immobilization combination) method of the microbial agent to improve the stability of the microbial agent. The preparationmethod is simple in operation and easy to be popularized and applied in industry.

The invention provides efficient phosphorus-dissolution promotion bacteria and a microbial agent prepared from the same. The effective components of the microbial agent include Pantoea agglomerans P36 and Trichoderma harzianum. In the invention, the widely-adaptable efficient phosphorus-dissolution yield-increasing bacteria Pantoea agglomerans P36 and the efficient anti-crop disease Trichoderma harzianum are organically combined; by applying the composite bacterial agent provided by the invention, the effect of the microorganism in preventing soil-borne diseases is sufficiently exerted while efficiently utilizing the soil phosphorus and improving the utilization rate of phosphate fertilizer; an effect of increasing yield by dissolving phosphorus and resisting diseases is realized; and theinvention has tremendous application values in agriculture.

The invention discloses a vegetable waste composting bacterial agent, a using method thereof and an organic substrate prepared from the vegetable waste composting bacterial agent. The vegetable waste composting bacterial agent is prepared from the following components in parts by weight: 1-3 parts of phanerochaete chrysosporium, 1-3 parts of trichoderma harzianum, 1-3 parts of bacillus subtilis and 1-3 parts of bacillus mucilaginosus, wherein the effective viable count of the complex bacterial agent is up to 4*10<8> / g. The vegetable waste composting bacterial agent can be used for fermenting and composting vegetable wastes and resisting to cucumber fusarium wilt and has the fermentation period of 25 days for treating vegetable wastes and more than 50 days for treating uninoculated vegetable wastes. The organic substrate is prepared through fermenting by using a special process after adding 1kg of composting bacterial agent into every 5 cubic meters of vegetable wastes, wherein the vegetable wastes comprise the following components in parts by weight: 40-60 parts of eggplant straw, 20-40 parts of cucumber vine, 5-15 parts of Chinese cabbage leaves and 5-15 parts of brown coal, wherein the organic substrate can be used as a seedling substrate of tomatoes, has the emergence rate of 92% and has the same effect as a seedling substrate sold on the market.

The invention relates to the technical field of biology, and particularly relates to a straw decomposition agent and a producing method thereof. The straw decomposition agent includes peat and a microbial flora containing a bacteria active ingredient, the bacteria active ingredient is bacillus, trophozoite or spore; wherein the microbial flora includes trichoderma harzianum, bacillus subtilis, saccharomyces cerevisiae and metarhizium anisopliae; and the microbial flora content is: 50-500 million / g peat. The straw decomposition agent plays the role of decomposition of straw and also has the effect of inhibition of the growth and reproduction of diseases and insect pests.

The invention discloses a method for producing trichoderma solid spawn by taking an amino acid hydrolytic solution and rice straw as raw materials. A filamentous fungus Trichoderma harzianum with a good plant rhizosphere growth-promoting effect is utilized, the rice straw rich in lignocellulose and abandoned livestock amino acid hydrolytic solution nutrients are fully utilized under the culture condition of 28 DEG C, rapid growth and spore production are achieved, after solid fermentation is conducted for 5 days to 6 days, the number of chlamydospores of trichoderma guizhouense NJAU4742 reaches up to 2.4*10<10> spore / g, the solid spawn is directly added into a decomposed organic fertilizer, a trichoderma amino acid bio-organic fertilizer of which the number of stable and effective living bacteria is larger than 2*10<8> can be rapidly produced, and the bio-organic fertilizer has a very good rhizosphere growth-promoting effect on tomatoes.