418 results about "Trichoderma harzianum" patented technology

The invention relates to the technical field of fertilizer preparation, and discloses an ecological balance fertilizer for yield increase and disease resistance of peanuts and a preparation method of the ecological balance fertilizer. The preparation process comprises the following steps: constructing a PLGA core containing trichoderma harzianum spores and magnesium carbonate through a micro-fluidic chip low-temperature water-in-oil emulsion method; coating bacillus amyloliquefaciens and potassium silicate with ultraviolet-induced cross-linked temperature-sensitive hydrogel to form a middle layer; loading rhizobium by using aminated mesoporous S < iO2 >, and spraying and assembling an enzyme response shell through a fluidized bed; finally, the hydroxyapatite nanorod and a binder are combined and granulated and formed through a fluidized bed. Precise controlled release of functional bacteria and nutrients is realized by constructing a temperature-sensitive-enzyme response type release system, and the activity of a fungicide is guaranteed to be preserved by virtue of trichoderma harzianum-rhizobium synergistic interaction and silicon bond linkage soil remediation in combination with a low-temperature fluidized bed process, so that a'fertilizer-bacterium-soil 'ecological cycle system is finally constructed.

The invention discloses a functional microbial organic fertilizer for saline-alkali soil improvement and a preparation method and application thereof, and belongs to the technical field of fertilizers. In order to solve the technical problem that high salt ion content in saline-alkali soil causes multiple adverse effects on plant growth, the invention provides a functional microbial organic fertilizer for saline-alkali soil improvement. The functional microbial agent is prepared from bacillus amyloliquefaciens, insecticidal cupriavidus, trichoderma harzianum, halophilic bacillus grignard and halotolerant bacillus, and the functional microbial carrier organic fertilizer is added with furfural residues and citric acid residues. The functional microbial organic fertilizer provided by the invention has the functions of improving the microbial abundance of saline-alkali soil, improving the physicochemical properties of the soil, adjusting the pH value of the soil, increasing the proportion of soil aggregates and increasing the contents of rapidly available phosphorus and rapidly available potassium. The functional microbial organic fertilizer provided by the invention can be applied to effectively improving saline-alkali soil.

The invention provides bacillus velezensis NR10, and the preservation number of the bacillus velezensis NR10 is CGMCC (China General Microbiological Culture Collection Center) NO.31771. The bacillus velezensis NR10 is a strawberry endophyte, has good bacteriostatic activity, has good antagonistic action on botrytis cinerea, fusarium oxysporum, colletotrichum gloeosporioides, colletotrichum oxysporum, trichoderma harzianum, trichoderma longibrachiatum and the like, is a potential biological preparation for preventing and treating strawberry diseases and the like, and has broad application prospects. And the bacteriostatic action of the bacillus velezensis NR10 has thermal stability. In addition, the bacillus velezensis also has good capacities of producing siderophores, fixing nitrogen and dissolving potassium, producing protease and cellulase and the like. The bacillus velezensis NR10 provided by the invention has wider-spectrum disease resistance, wide development space and good development and application prospects.

The invention relates to the technical field of microbial agents, in particular to a composite microbial agent and application of the composite microbial agent in prevention and treatment of forest root diseases, and the composite microbial agent comprises the following components in parts by weight: 15-25 parts of Trichoderma harzianum ATCC 20847; 10 to 20 parts of bacillus subtilis ATCC 605, 10 to 20 parts of bacillus subtilis ATCC 605, 10 to 20 parts of bacillus subtilis ATCC 606 and 10 to 20 parts of bacillus subtilis ATCC 607. 5-10 parts of a seaweed extract; 3 to 8 parts of chitosan; 2-5 parts of humic acid; 1 to 3 parts of glycine betaine; 0.5 to 1.5 parts of a trace element compound; 30 to 40 parts of perlite powder; according to the present invention, the fertilizer has characteristics of significant inhibition effect on a variety of forest root pathogenic bacteria, wide prevention and control spectrum, and long-term and stable disease prevention effect by adjusting the rhizosphere micro-ecosystem. Diseases are prevented and controlled, and the growth and root development of the forest trees are remarkably promoted.

Methods are provided for enhancing the health, growth and / or sturdiness of turf grasses by applying a combination of microbes and / or their growth by-products to the turf grass roots and / or to soil. Specifically, in one embodiment, the subject invention utilizes Trichoderma harzianum and a strain of Bacillus amyloliquefaciens. The methods can further be used to improve maintenance and upkeep of natural turf grass fields used in, for example, athletics and recreation, as well as to increase the functionality and safety of these natural turf grass fields.

This invention discloses a microbial inoculant for controlling peanut root rot and its preparation method, belonging to the field of microbial materials technology. This invention screened and obtained a *Trichoderma harzianum* strain with highly efficient inhibitory effects against the pathogen causing peanut root rot. Firstly, the strain itself exhibits antagonistic activity against the peanut pathogen. Secondly, it was prepared as a spore suspension and fermented in solid-state with rice husks and wheat bran. During the solid-state fermentation process, the metabolites produced by *Trichoderma harzianum* promote the growth and development of plant roots, increasing plant biomass and yield. This microbial inoculant effectively controls peanut root rot, exhibiting good inhibitory effects against the pathogen, thus improving peanut quality and disease resistance. Furthermore, it improves soil ecological activity to a certain extent, making it worthy of application and promotion.

The invention discloses a special fertilizer composition for high-yield cultivation of sugarcane, and belongs to the technical field of sugarcane planting. Comprising a disease-resistant fertilizer and an insect-resistant fertilizer; wherein the disease-resistant fertilizer comprises the following raw materials in parts by mass: 50 parts of diatomite, 5-10 parts of biomass charcoal, 1-2 parts of a compound microbial agent, 150-170 parts of an organic fertilizer and 50-60 parts of an inorganic fertilizer; wherein the compound microorganism bacterium agent comprises a bacillus subtilis bacterium agent, a trichoderma harzianum bacterium agent, a trichoderma viride bacterium agent and a paecilomyces lilacinus bacterium agent; the insect prevention fertilizer comprises beauveria bassiana suspension and matrine in a volume ratio of (3.5-4.5): 1. According to the fertilizer composition special for high-yield cultivation of sugarcane, the synergistic effect of microorganisms is utilized, the broad-spectrum inhibition effect on microorganisms in sugarcane continuous cropping disease soil can be achieved, the kieselguhr and the biomass charcoal cooperate to form a porous substance, nutrient elements are adsorbed in pores, the slow-release effect is improved, the fertilizer efficiency is prolonged, and the effect is good. Moreover, the soil structure can be improved, and the disease resistance of the sugarcane during continuous cropping is greatly improved through the synergistic interaction with the microbial complex microbial inoculants.

The invention relates to the field of microorganisms, and particularly provides three types of trichoderma strains, namely, a trichoderma harzianum strain T31818-A (the preservation number is GDMCC (Gramineae Microorganism Center for March Center) NO.64772), a trichoderma gramineae strain YNE00410-A (the preservation number is GDMCC NO.64822), a trichoderma gramineae strain YNE00489-A (GDMCC NO.64775), a trichoderma viride strain YNE00489-A (the preservation number is GDMCC NO.64775) and a trichoderma viride strain YNE00489-A (the preservation number is GDMCC NO.64775). The invention also provides application of the three trichoderma strains in agriculture: promoting plant growth, preventing and treating plant diseases and improving plant disease resistance.

The present invention discloses a preparation for promoting strontium absorption in blueberries, belonging to the technical field of blueberry cultivation. The preparation consists of citric acid, potassium fulvate and Trichoderma harzianum. On the one hand, the preparation promotes the biological availability of strontium in strontium-rich soil, and on the other hand, by improving soil air permeability, it promotes the growth of blueberry roots and enhances the strontium absorption capacity of blueberry roots, thereby increasing the strontium content in blueberry fruits. The preparation for promoting strontium absorption in blueberries provided by the present invention has a simple formula, a simple process, and the raw materials used are all common raw materials, with a low cost. Applying the preparation for promoting strontium absorption in blueberries provided by this application can not only increase the strontium content in blueberry fruits, but also increase the yield of blueberries, which is of great significance for blueberry cultivation.

The invention discloses a special bacterial fertilizer for sorghum and application of the special bacterial fertilizer in prevention and control of sorghum diseases, and belongs to the technical field of microbial fertilizers and agriculture. The special bacterial fertilizer for sorghum comprises the following components in parts by weight: 8-10 parts of a composite microbial agent, 40-50 parts of sorghum residues, 5-10 parts of bentonite, 1-2 parts of potassium humate, 1-2 parts of composite trace elements, 10-20 parts of sawdust and 10-20 parts of rice bran, wherein the complex microbial inoculant consists of a bacillus subtilis microbial inoculant, a rhizobium microbial inoculant, a bacillus velezensis microbial inoculant and a trichoderma harzianum microbial inoculant. The special bacterial fertilizer for sorghum provided by the invention contains rich microorganisms and nutrient substances, and can effectively prevent and control sorghum diseases and enhance the disease resistance of sorghum. The components such as microorganisms and organic acids in the special bacterial fertilizer for sorghum can promote the formation of soil organic matters, improve the soil structure, improve the soil quality and effectively improve the growth environment of sorghum.

The invention discloses an African trichoderma harzianum strain 6-5 and application thereof, the disclosed strain is named as Trichoderma harzianum, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No. 41930. The bacterial strain has obvious bacteriostatic activity on crop pathogenic bacteria such as colletotrichum capsici, apple cyst, botrytis cinerea, alternaria longibrachiata and phytophthora capsici, and fermentation liquor of the bacterial strain has good prevention and control effects on apple anthracnose leaf blight, apple bitter rot and apple tree canker.

The invention relates to the technical field of fertilizers, and discloses a rice sheath blight disease prevention fertilizer, which comprises a fertilizer, medium trace elements, an adhesion functional carrier, a functional additive, and a microbial agent or a bactericide, the fertilizer comprises a nitrogen-phosphorus-potassium compound fertilizer, an original fertilizer, calcium superphosphate and ammonium sulfate, wherein the original fertilizer comprises decomposed livestock and poultry manure, crop straw compost, bean cakes and rape seed cakes; the medium trace elements comprise boric acid, copper sulfate and borax; the microbial agent comprises a bacillus subtilis microbial agent and a trichoderma harzianum microbial agent; the bactericide comprises validamycin wettable powder, a thifluzamide suspending agent and a tebuconazole emulsion in water. Through the components of the fertilizer, the occurrence rate of rice sheath blight disease can be remarkably reduced, the yield and quality of rice can be improved, the microbial agent can increase the number and activity of beneficial microorganisms in soil, the soil structure and fertility are improved, and the yield and quality of rice are improved. A good soil condition is created for rice growth, so that the rice has a better resistance effect.

The invention belongs to the technical field of microorganisms, and particularly relates to a synthetic flora for promoting growth of macadamia nuts as well as a construction method and application of the synthetic flora. The invention relates to a synthetic flora. The synthetic flora is prepared by mixing bacillus subtilis X17, pseudomonas fluorescens X62 and trichoderma harzianum Z27 in equal volume. According to the invention, strains are separated and screened from healthy rhizosphere soil, and the strains which grow well are subjected to a pairwise confrontation test, so that the strains which have no antagonism in pairwise are screened out. According to molecular biology identification, three strains with the growth promoting effect, namely bacillus subtilis X17, pseudomonas fluorescens X62 and trichoderma harzianum Z27, are screened, then the three strains are constructed into a synthetic flora, pot experiment verification shows that the constructed synthetic flora can promote growth of macadimia nut plants and accumulation of biomass, meanwhile, soil can be activated, the yield of macadimia nut plants is increased, and the yield of macadimia nut plants is increased. The soil nutrient content, the enzyme activity and the microbial biomass carbon, nitrogen and phosphorus content are improved, and support can be provided for planting and economic development of macadamia nuts.

The invention provides a microbial complex microbial inoculant and application thereof in prevention and treatment of plant diseases, the microbial complex microbial inoculant comprises the following strains by mass: 30%-50% of bacillus subtilis; 20%-40% of bacillus amyloliquefaciens; 10%-30% of pseudomonas fluorescens; 5%-15% of trichoderma harzianum; and 2%-5% of an auxiliary agent. According to the microbial complex microbial inoculant disclosed by the invention, a multiple prevention and control mechanism of'bacteriostasis-competition-parasitism 'is constructed through the synergistic effect of various strains, and meanwhile, the double-layer effect of thallus protection and function enhancement is realized through the assistance of the auxiliaries, so that the prevention and control effect of the prepared microbial complex microbial inoculant is greatly improved.

The invention discloses a continuous cropping Chinese cabbage planting method based on a microbial agent and application, and belongs to the technical field of crop cultivation. According to the planting method, Chinese cabbage and garlic are intercropped according to the ratio of 1: 1, and a trichoderma harzianum agent is applied. Results show that the planting method can significantly increase the growth indexes such as the plant height, the root length, the leaf chlorophyll content and the soluble sugar content of the Chinese cabbage; the soluble protein content, the peroxidase activity, the superoxide dismutase activity and the malondialdehyde content of the Chinese cabbage leaves are obviously reduced. Meanwhile, the content of rapidly available phosphorus, rapidly available potassium, effective calcium and other substances in the soil can be increased; the activities of urease, catalase and alkaline phosphatase in the soil are also obviously improved. In addition, the number of fungi, bacteria and actinomycetes in the soil is also remarkably increased. Therefore, according to the planting method, the quality of continuous cropping soil can be improved, Chinese cabbage growth is promoted, and theoretical support is provided for healthy and sustainable development of the Chinese cabbage industry.

The invention belongs to the technical field of coastal saline-alkali soil remediation, and particularly relates to a composite microbial agent for coastal saline-alkali soil remediation. The microbial agent is in a granule form, and comprises a carrier, which is a mixture of straw powder and duck manure; the active flora comprises bacillus licheniformis, bacillus megaterium, brevibacillus laterosporus, trichoderma harzianum spore powder, paenibacillus polymyxa, halomonas and pseudomonas fluorescens, the active flora is adsorbed in the carrier, and the halomonas and the pseudomonas fluorescens are subjected to embedding treatment; and the spraying film is sprayed on the surface of the carrier. Through the synergistic effect of multiple strains, the improvement effect on saline-alkali soil and the growth promoting effect on crop growth are improved, and the defect that the function of a single strain is limited is overcome.

The invention discloses a method for inducing plant disease resistance by using a hydrolase active mutant of xyloglucan specific glucanase XEG1. A pichia pastoris eukaryotic expression system is utilized to obtain the purified hydrolase activity mutant XEG1E136D protein of the phytophthora sojae core pathogenic factor xyloglucan specific glucanase XEG1, and the hydrolase activity mutant XEG1E136D protein does not have the hydrolase activity of plant cell walls and can activate the systemic resistance of plants. The XEG1E136D is overexpressed into a biocontrol bacterium trichoderma harzianum by utilizing an agrobacterium-mediated fungal transformation optimization system, and the XEG1E136D is proved to be capable of secreting XEG1E136D protein to the outside of cells and activating the broad-spectrum systemic resistance of plants. The XEG1E136D protein expressed by the trichoderma harzianum can be used as a plant vaccine to improve the prevention and treatment effect, and the trichoderma harzianum XEG1E136D protein has a wide application prospect in the field of plant disease prevention and treatment.

The invention relates to the technical field of microbial fermentation feeds, in particular to a method for producing a protein feed through solid-state fermentation of distiller's grains based on Trichoderma harzianum pretreatment. According to the method, the vinasse is pretreated by the crude enzyme prepared from trichoderma harzianum, and the formula and fermentation conditions of an enzyme production culture medium are optimized, so that the filter paper enzyme activity of cellulase is remarkably improved. By adding the surfactant into a fermentation system and optimizing the variety of the surfactant, the yield of the fermented monosaccharide is improved, and the problem of musty taste in a traditional fermented product is solved while the degradation efficiency of lignocellulose is improved. Furthermore, a synchronous saccharification and fermentation process is adopted, vinasse is fermented through compounded saccharomycetes and crude enzyme liquid, the trichoderma harzianum fermented protein feed is prepared and obtained, digestion and absorption of animals are easier, lactic acid fermentation is conducted on the trichoderma harzianum fermented protein feed again through lactobacillus fermentum, the flavor of the feed is improved, the bitter taste of the feed is reduced, and the sour taste is improved. The method realizes efficient utilization of vinasse resources.

The invention provides an acid-blocking and aluminum-controlling composite modifier and a preparation method and application thereof, the modifier is composed of microcrystalline activated minerals, a silicon-calcium material, a biomass alkaline material and a microbial agent, and the microbial agent comprises rhizobium, trichoderma harzianum and pseudomonas fluorescens. The specific raw material ratio can be adjusted according to different requirements, and the effect of materials with different ratios on improving the soil acidity and improving the crop yield and quality is researched by adopting field trials, so that an acid soil conditioner formula is formed; the preparation method comprises the steps of ultrafine grinding, microcrystal activation, hybridization, mixing and the like, the microcrystal activation adopts an activator solution for treatment, and an organic binder is added in the hybridization process to form an organic-inorganic hybrid structure. The composite modifier can effectively improve the physical action effect and the chemical reaction speed of the acid conditioner, and can be used for improving acid soil.

The invention relates to a soil remediation complex microbial inoculant composition and a preparation method thereof, and belongs to the technical field of soil remediation. Comprising the following raw materials: 10-15 parts of a microbial complex inoculant, 40-50 parts of a porous carrier, 20-25 parts of an organic matter and 3-5 parts of a surface protective agent, wherein the porous carrier is obtained by treating diatomite with phytic acid and polylysine. Paenibacillus polymyxa, trichoderma harzianum, bacillus subtilis and azotobacter chroococcum are taken as a microbial compound inoculant, modified diatomite is taken as a porous carrier, and the microbial compound inoculant is mixed with organic matters under the action of a protective agent, so that the soil remediation compound inoculant composition which can effectively improve soil and is excellent in strain activity is obtained.

The invention belongs to the field of microorganisms, and particularly relates to a banana endophyte compound fungicide as well as a preparation method and application thereof. According to the invention, a novel endophytic bacillus velezensis JDB15 strain and an endophytic trichoderma harzianum JDL4 strain are separated from a banana plant. The trichoderma harzianum JDL4 can inhibit botrytis cinerea, fusarium graminearum, rhizoctonia solani, peach anthracnose pathogen, peach brown rot pathogen and alternaria alternata, and has a good broad-spectrum antibacterial effect. Meanwhile, after the filtrate of the fermentation liquor of the trichoderma harzianum JDL4 and the bacillus velezensis JDB15 are compounded, the antibacterial effect is remarkably improved, and the control effect on banana fusarium wilt, pyricularia oryzae, tomato fusarium wilt and corn bipolaris maydis is also remarkably improved. The compound bacterial agent has wide biocontrol bacterial activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other plant diseases.

The invention discloses dsRNA of a targeted BdSpt7 gene, a biocontrol agent and application of the dsRNA. The sequence of the dsRNA of the targeted BdSpt7 gene is as shown in SEQ ID No.2. The invention further discloses a preparation method of the biocontrol agent of the targeted BdSpt7 gene. The active ingredient of the biocontrol agent is the dsRNA of the target BdSpt7 gene. The invention also provides an application of the botryosphaeria dothidea BdSpt7 gene in prevention and treatment of apple botryosphaeria dothidea, and a nucleotide sequence of the botryosphaeria dothidea BdSpt7 gene is shown as SEQ ID No.1. The invention also provides an African trichoderma harzianum engineering strain for expressing dsRNA, the African trichoderma harzianum engineering strain is integrated with a dsRNA expression box for interfering gene expression of a target gene BdSpt7, the dsRNA expression box is shown as SEQ ID No.1, and the African trichoderma harzianum is an African trichoderma harzianum with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 41709. The dsRNA targeting the BdSpt7 gene can significantly reduce the pathogenicity of the physalospora piricola through interference, and the African trichoderma harzianum engineering strain can colonize on the plant surface and continuously express the dsRNA so as to play a role in preventing and treating the physalospora piricola, and is green and environment-friendly.

The invention relates to the technical field of biocontrol microorganisms, in particular to trichoderma harzianum and application thereof. The trichoderma harzianum is preserved in the China General Microbiological Culture Collection Center (CGMCC) on August 20, 2024, and the preservation number is CGMCC No.41490. The trichoderma harzianum is a trichoderma harzianum strain. The invention also provides a culture, a fermentation broth, a bacterial suspension, a bacterial agent and bacterial powder containing the strain, and a fermentation supernatant prepared from the strain. In addition, the invention also provides application of the strain and a culture, a fermentation broth, a bacterial suspension, a bacterial agent, bacterial powder and a fermentation supernatant thereof in the fields of plant growth promotion and rot disease prevention and treatment. According to the application, the trichoderma harzianum is screened from the ginger rot soil, the trichoderma harzianum not only can effectively inhibit harmful pathogenic bacteria such as fusarium and the like, but also can promote seed germination and normal growth of crops and increase the yield of the crops, and the trichoderma harzianum has a good effect on growth promotion of the ginger and prevention and treatment of the rot in field application.

A functional microbial organic fertilizer for improving saline-alkali land, and its preparation method and application, belong to the field of fertilizer technology. In order to solve the technical problem that the high salt ion content in saline-alkali soil has multiple adverse effects on plant growth, the present invention provides a functional microbial organic fertilizer for improving saline-alkali land, which is prepared by a functional microbial agent prepared from Bacillus amyloliquefaciens, Copperbacterium insecticide, Trichoderma harzianum, Halobacterium gesneri and Bacillus halogenide, and a functional microbial carrier organic fertilizer added with furfural residue and citric acid residue. The functional microbial organic fertilizer provided has the functions of increasing the abundance of saline-alkali soil microorganisms, improving soil physical and chemical properties, regulating soil pH, increasing the proportion of soil aggregates, and increasing the content of available phosphorus and available potassium. The functional microbial organic fertilizer provided by the present invention can be used to effectively improve saline-alkali soil.

The present invention belongs to the field of Trichoderma secondary metabolites, and discloses a natural product derived from Trichoderma harzianum, a preparation method and an application thereof. The natural product has a structure shown in Formula I. The present invention separates and purifies the Trichoderma harzianum secondary metabolites to obtain the Trichoderma harzianum source natural product LU-11, which can be used to inhibit the growth of Fusarium graminearum PH-1, a wheat head blight disease, and induce and improve the disease resistance of wheat, providing a certain theoretical basis for the development of biological pesticides.

The invention discloses a method for promoting growth or resisting diseases of oilseed rape. The research finds that any one or a combination of more of bacillus subtilis B.subtilis 168, trichoderma harzianum T.harzianum 25852 and trichoderma harzianum T.harzianum 52623 can effectively promote the growth of rape seedlings, and the growth mainly shows that the plant stem diameter, the plant height and the increase of the biomass of the overground part and the underground part. Wherein the trichoderma harzianum T.harzianum 25852, and the combination of the bacillus subtilis B.subtillis 168 and the trichoderma harzianum T.harzianum 25852 also have a relatively good sclerotinia rot prevention and treatment effect. The microorganism provided by the invention has the characteristics of high efficiency, no pollution, difficult generation of drug resistance and the like in the aspect of oilseed rape production, has great application potential, and has important significance for promoting the improvement of oil productivity and green low-carbon efficient production in China.

The invention provides an industrialized seedling production and cultivation method for shortening the growth period of fritillaria cirrhosa. The industrialized seedling production and cultivation method comprises the following steps: step 1, selecting cultivation germplasm and a cultivation environment; step 2, purchasing a plurality of culture racks according to the size of a plant, and arranging the plurality of culture racks into a plurality of rows; an environment control integrated master controller is also arranged in the plant; each layer of shelf of each culture shelf is provided with seedling culture equipment; hole trays are placed in the seedling raising equipment; 3, seeds with the embryo rate (embryo length / endosperm length) larger than 90% are disinfected and then sown in a hole tray, and the seeds are covered with a matrix by 1-1.5 cm; 4, a nutrient solution is added into the seeds, and after the seeds sprout, fish protein water-soluble fertilizer and bacillus subtilis and trichoderma harzianum compound bacterial fertilizer are additionally applied; and step 5, controlling growth conditions, setting the growth cycle of the bulbus fritillariae cirrhosae to be 120-160 days, wherein 100-150 days are a growth period and 10-30 days are a dormancy period, and performing cultivation to realize cultivation of 2-3 growth cycles within one year.

The invention belongs to the technical field of agricultural microbial control, and particularly relates to a growth-promoting and disease-preventing compound microbial agent and a preparation method and application thereof, and the compound microbial agent specifically comprises microbial powder, a growth auxiliary material and a drying agent; the microorganisms comprise vermiform yellow, trichoderma harzianum, bacillus velezensis, bacillus licheniformis and brevibacillus laterosporus. According to the compound microbial agent, through the synergistic effect of multiple strains, the control effect of the compound microbial agent on verticillium dahliae is enhanced, and the defects that a single biocontrol bacterium is single in biocontrol mechanism, poor in environment adaptability, incapable of being colonized in soil for a long time and unstable in control effect are overcome; the compound microbial agent can increase the yield of cotton and pepper and improve the quality of cotton fibers, and provides scientific and technological support and decision service for realizing green, environment-friendly, cost-saving and efficiency-increasing modern agriculture.

The invention belongs to the technical field of microorganisms, and particularly discloses trichoderma harzianum NBU-1 and application thereof. The Trichoderma harzianum NBU-1 is preserved in the Guangdong Microbial Culture Collection Center on March 26, 2025, the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCCNO: 66053. The invention discloses trichoderma harzianum NBU-1 and application thereof, the trichoderma harzianum NBU-1 is applied to prevention and treatment of wheat sharp eyespot, and the activity of rhizoctonia cerealis R0301 and the growth of scab are effectively inhibited.

The invention relates to the field of fertilizers, and discloses a microbial agent for replacing a chemical fertilizer and a preparation method thereof, and the microbial agent comprises the following components: trichoderma harzianum with a specific dosage of 0.5-1 billion CFU / g, bacillus mucilaginosus with a specific dosage of 10-20 billion CFU / g, and bacillus subtilis with a specific dosage of 21-20 billion CFU / g. The microbial agent and a carrier are prepared into a proper application concentration, the mixture is mixed with farmyard manure according to a weight ratio of 1: 1250-2500, and the mixture is applied to crops, so that more excellent growth promoting and yield increasing effects can be achieved compared with the mixture of a compound fertilizer and the farmyard manure, an unexpected technical effect is achieved, and the microbial agent is suitable for large-scale popularization and application.