110 results about "Trichoderma harzianum" patented technology

The invention discloses dsRNA of a targeted BdSpt7 gene, a biocontrol agent and application of the dsRNA. The sequence of the dsRNA of the targeted BdSpt7 gene is as shown in SEQ ID No.2. The invention further discloses a preparation method of the biocontrol agent of the targeted BdSpt7 gene. The active ingredient of the biocontrol agent is the dsRNA of the target BdSpt7 gene. The invention also provides an application of the botryosphaeria dothidea BdSpt7 gene in prevention and treatment of apple botryosphaeria dothidea, and a nucleotide sequence of the botryosphaeria dothidea BdSpt7 gene is shown as SEQ ID No.1. The invention also provides an African trichoderma harzianum engineering strain for expressing dsRNA, the African trichoderma harzianum engineering strain is integrated with a dsRNA expression box for interfering gene expression of a target gene BdSpt7, the dsRNA expression box is shown as SEQ ID No.1, and the African trichoderma harzianum is an African trichoderma harzianum with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 41709. The dsRNA targeting the BdSpt7 gene can significantly reduce the pathogenicity of the physalospora piricola through interference, and the African trichoderma harzianum engineering strain can colonize on the plant surface and continuously express the dsRNA so as to play a role in preventing and treating the physalospora piricola, and is green and environment-friendly.

The invention discloses a trans-boundary synthetic flora (SMC) for preventing and treating wheat stem rot as well as preparation and application of the trans-boundary synthetic flora. The synthetic bacterial flora is prepared by compounding Trichoderma harzianum T19 with the preservation number of CCTCC (China Center for Type Culture Collection) No: M 20251896, bacillus subtilis BS-Z15 with the preservation number of CCTCC No: M 20251892, two strains of growth promoting bacillus PM9 and PM16, and two strains of auxiliary bacillus S8 and S32 according to a specific proportion. Compared with an existing single antagonistic bacterial agent, the synthetic flora disclosed by the invention has the advantages that the bacteriostasis rate of the synthetic flora on Fusarium pseudograminearum under a non-sterile soil condition can reach 70% or above, and the disease index is reduced by 70.01%; meanwhile, the plant height of wheat is increased by 28.63%, the root length is increased by 24.53%, the fresh weight is increased by 181.21%, the dry weight is increased by 184.64%, the thousand seed weight is increased by 10.39%, and the rhizosphere microbial community structure and the soil enzyme activity are remarkably improved. The method can be used for green prevention and control and yield increase of basal stem rot of wheat and other gramineous crops.

The application discloses a kind of fermentation complex microbial inoculant and preparation method and application thereof, the fermentation complex microbial inoculant includes the following weight parts of component: bacillus subtilis microbial inoculant 2-5 parts, bacillus mucilaginosus microbial inoculant 5-10 parts, yeast microbial inoculant 2-10 parts, trichoderma harzianum microbial inoculant 10-20 parts, aspergillus niger microbial inoculant 10-20 parts and streptococcus thermophilus microbial inoculant 2-5 parts;The fermentation complex microbial inoculant of the application is prepared by selecting each strain and amount, is added to pigeon manure and is fermented to prepare organic fertilizer, can better decompose macromolecular substance, significantly improve the organic matter and total nitrogen content in preparation organic fertilizer, and can better improve seed germination rate;In addition, the preparation method of pigeon manure organic fertilizer of the application is simple in process, easy to operate, and convenient for production.

The invention discloses a bio-organic fertilizer and application thereof, belongs to the field of bio-organic fertilizers, and solves the problem of environmental pollution caused by stacking or landfill of potato residues. The bio-organic fertilizer comprises potato residue zymolyte, a decomposed organic fertilizer and a compound microbial agent, the compound microbial agent is prepared from trichoderma harzianum, bacillus subtilis and bacillus velezensis; the preparation method comprises the following steps: mixing the potato residue zymolyte, the decomposed organic fertilizer and the compound microbial agent, controlling the water content to be 40-50%, fermenting at 40-50 DEG C for 10-15 days, and airing and crushing a fermented product to obtain a finished product. The invention relates to application of a bio-organic fertilizer in quality improvement and yield increase of crops. According to the method, the potato residues which are difficult to degrade are converted into organic fertilizers with high added values through a directional enzymolysis technology, so that the problem of environmental pollution is solved; the compound microbial flora and the enzymolysis product have a synergistic effect to accelerate the conversion of organic matters; the fertilizer is rich in active organic matters and functional microorganisms, and can significantly improve the soil structure.

The invention discloses a microbial composition for preventing and treating vomitoxin as well as a preparation method and application of the microbial composition. The microbial composition for preventing and treating vomitoxin is mainly prepared by mixing a component I and a component II according to a mass ratio of (50.0-0.1): (50.0-99.9), the component I comprises trichoderma harzianum powder, trichoderma asperellum powder and penicillium bayi powder; and the component II comprises potassium phosphite, alkene potassium phosphate and potassium tripolyphosphate. The preparation method comprises the following steps: adding the component I and the component II into a stirrer, and stirring and mixing to obtain the coating. The invention also discloses an application of the microbial composition for preventing and treating vomitoxin. The microbial composition disclosed by the invention can generate metabolites with a biocontrol effect, has efficient inhibitory activity on fusarium, can reduce generation of vomitoxin, can effectively regulate diversity of soil microorganisms, realizes crop yield increase and soil improvement, and is green and environment-friendly. The method and application are simple, the cost is low, and the method is suitable for agricultural application.

The invention belongs to the technical field of biological phosphorus-silicon fertilizers, and particularly relates to a liquid complex microbial inoculant capable of repairing heavy metals and activating phosphorus and silicon and a biological phosphorus-silicon fertilizer thereof. The liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria Cupriavidus and Ochrobactrum, nitrogen-fixing bacteria Azospirillum brasilense, phosphate solubilizing bacteria Bacillus megaterium and Pseudomonas, potassium solubilizing bacteria Bacillus mucilaginosus, growth-promoting bacteria Pseudomonas fluorescens, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants and microbial inoculants. The biocontrol bacteria are trichoderma harzianum and bacillus subtilis, and the synergistic bacteria are saccharomyces cerevisiae; the biological phosphorus-silicon fertilizer is prepared from the liquid complex microbial inoculant. According to the biological phosphorus-silicon fertilizer provided by the invention, through scientific compatibility of various functional strains, heavy metal remediation and soil phosphorus and silicon solubilization are realized, absorption of phosphorus and silicon in soil is promoted, and the crop yield is increased.

The application provides a Rhodiola kirilowii root rot harmless prevention and control method and relates to the technical field of plant disease prevention and control. The Rhodiola kirilowii root rot harmless prevention and control method is characterized by the following steps: after seed disinfection, the seed is treated by immersion in a solution of a Yunnan sclerotia of Sclerotinia spore powder extract, and a fermentation substrate of Bacillus subtilis and Bacillus amyloliquefaciens is used, and then the roots are irrigated with a solution of a Yunnan sclerotia of Sclerotinia spore powder extract and a Trichoderma harzianum liquid. The method can effectively prevent and control the Rhodiola kirilowii root rot, reduce the use of chemical pesticides, reduce pesticide residues, and improve the safety of planting.

The invention discloses a Chinese angelica out-of-season single seedling raising method suitable for mechanical transplanting and application thereof, and belongs to the field of Chinese herbal medicine seedling raising and cultivation. According to the method, a sunlight greenhouse or a gutter-connected greenhouse with the altitude being 2300 m or above and the lowest temperature being larger than or equal to 8 DEG C in winter is selected, field soil without continuous cropping obstacles is matched with fried rapeseeds, trichoderma harzianum and bacillus subtilis to prepare a special matrix, a seedling raising paper book with the single-hole diameter being 19-25 mm and the height being 12 cm is adopted, pelletized seeds are dibbled in a double-grain mode from late November to early December, and the seeds are sown in the seedling raising paper book with the single-hole diameter being 19-25 mm and the height being 12 cm. Through management of precise temperature and humidity regulation and control, foliage dressing, seedling hardening and leaf cutting and the like, standardized single seedlings are cultivated and transplanted through a transplanter. According to the method, the early bolting rate of the angelica sinensis is reduced, the seedling uniformity and the transplanting survival rate are improved, the transplanting efficiency is improved, the labor cost is reduced, the yield of the angelica sinensis can be increased, efficient utilization of out-of-season resources is achieved, and support is provided for industrial large-scale standardized development.

The invention discloses a microbial compound fermentation inoculant, a straw fermentation product and application of the straw fermentation product, and belongs to the technical field of agricultural microorganisms. The preparation method comprises the following steps: compounding bacillus subtilis, trichoderma harzianum and trichoderma longibrachiatum to form a microbial compound fermentation inoculant; when the microbial compound fermentation inoculant is used for fermenting ginger straws, the content of pyrogallic acid in a prepared straw fermentation product can be synergistically improved. The pyrogallic acid has an obvious antibacterial effect on fusarium moniliforme and fusarium laminatum which cause the apple continuous cropping obstacle; the growth of apple seedlings planted in continuous cropping soil can be effectively promoted. Therefore, the straw fermentation product disclosed by the invention can be used for preventing and treating apple continuous cropping obstacles, improving the biomass and root activity of apple seedlings under continuous cropping conditions and reducing the quantity of harmful fusarium in continuous cropping soil.

This invention belongs to the field of gene technology for disease control. This invention provides a method for preparing biocontrol engineered bacteria using *Trichoderma harzianum* as a dsRNA vector, comprising the following steps: determining the target gene based on the pathogenic fungus; using the target gene as an arm sequence, and centering on the VdTublin intron sequence of the *Verticillium dahliae* endogenous gene, connecting two identical arm sequences to both ends of the intron in a forward and reverse direction, respectively, to construct the target sequence; and transferring the target sequence into *Trichoderma harzianum* using ATMT to obtain engineered *Trichoderma harzianum* bacteria. The engineered strain obtained by this invention has a good inhibitory effect on the growth and pathogenicity of the corresponding fungus.

The invention provides a rapid dormancy breaking method for sand whip seeds and dormancy breaking sand whip seeds, and relates to the technical field of plant seed treatment.The method comprises the following steps that 1, physical initiation treatment is conducted, specifically, the disinfected sand whip seeds are placed in warm water at the temperature of 45-55 DEG C to be soaked for 10-14 h; (2) chemical initiation treatment: soaking the seeds treated in the step (1) in a gibberellin solution with the mass concentration of 0.03%-0.07% for 20-28 hours; (3) biological seed dressing treatment: carrying out seed dressing on the seeds treated in the step (2) and a trichoderma harzianum fungicide, wherein the mass of the fungicide is 0.05-0.15% of the mass of the seeds; according to the method, the dormancy mechanism of the sand penis seeds can be effectively broken, germination of the seeds is promoted, the germination rate of the sand penis seeds treated by the optimized method can reach 87.00% on the 21st day, the germination synchronism of the seeds can be higher, and the problem of irregular natural germination is solved.

The present application relates to the technical field of microorganism, and particularly relates to a microbial composition, a compound microbial agent and application.The microbial composition of the present application comprises Bacillus amyloliquefaciens, Bacillus brevis and Trichoderma harzianum, the microbial composition of the present application can simultaneously prevent and treat rice seed production process rice blast and storage process aspergillus flavus pollution, realize the whole life cycle protection of rice seed from production to storage, solve the problem that different agents need to be used to prevent and treat rice blast and aspergillus flavus pollution in the prior art.In addition, the compound microbial agent containing the microbial composition is safe, efficient, environmentally friendly, can replace chemical pesticides, reduce environmental pollution and pesticide residues, and the preparation method is simple, easy to industrial production, and has wide application prospect.

The invention relates to a microbial inoculum for improving the transplanting survival rate of xanthoceras sorbifolia bunge and a preparation method thereof.The microbial inoculum for improving the transplanting survival rate of xanthoceras sorbifolia bunge is characterized by comprising trichoderma harzianum, yeast cell walls and organic matter, and the organic matter contains monosaccharide or polysaccharide capable of being utilized by the trichoderma harzianum. The transplanting survival rate of xanthoceras sorbifolia bunge can be increased.

The invention belongs to the technical field of biological bacterial fertilizers, and particularly relates to a high-salt-tolerance microbial liquid complex microbial inoculant suitable for saline-alkali desertification land and a biological bacterial fertilizer thereof. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation of three stages: salt-tolerant repair bacteria Nocardia halophila, salt-tolerant functional bacteria Bacillus subtilis and Azospirillum brasilense, nitrogen-fixing functional bacteria Azotobacter chroococcum, phosphorus-solubilizing functional bacteria Pseudomonas fluorescens, and nitrogen-solubilizing functional bacteria Pseudomonas fluorescens. The bacillus mucilaginosus is used as a potassium-dissolving functional bacterium, the trichoderma asperellum is used as a biocontrol growth-promoting bacterium, and the trichoderma harzianum is used as a rhizosphere optimizing bacterium. The biological bacterial fertilizer is prepared from the liquid complex microbial inoculant. Through scientific compatibility of different strains provided by the invention, efficient improvement and crop yield increase of the saline-alkali desertification land are realized, and the strain has important economic and social values.

A method for purifying active polysaccharides from *Trichoderma harzianum* based on continuous chromatography is disclosed, relating to the food industry. The method includes the following steps: (1) extracting *Trichoderma harzianum* to obtain a polysaccharide extract; (2) fractionating and refining the polysaccharide extract using continuous chromatography to obtain a refined liquid; (3) removing salts from the refined liquid using membrane separation technology, concentrating the refined liquid, and freeze-drying it to obtain the refined active polysaccharides from *Trichoderma harzianum*. The active polysaccharides from *Trichoderma harzianum* obtained using this method achieve a purity of 80%-90% and a recovery rate of 75%-85%, respectively, and exhibit high activity. This method provides high product activity, high yield, high separation efficiency, and large processing capacity, significantly improving the utilization rate of raw materials, enabling continuous operation, and reducing labor costs.

The invention relates to the crossing field of agricultural biological control and fertilizer technology, and discloses a compound microbial pesticide fertilizer capable of simultaneously controlling root-knot nematode and soil-borne fungal diseases, the pesticide fertilizer has a core-shell structure, and comprises an inner inorganic nutrition core, a middle isolation buffer layer and an outer functional bacterial layer; the raw materials comprise inorganic nutrition core particles, a biological activation organic carrier, composite functional bacterial powder and a modified slow-release binder. Wherein the binder is prepared from oxidized starch and PVA through borax crosslinking, so that the particle strength is enhanced; the carrier is fermented and activated, and synergistic components such as azadirachtin are added. An organic isolation buffer layer is constructed through a layered coating process, osmotic pressure stress of inorganic salt on microorganisms is effectively blocked, and the problem of strain inactivation is solved; the synergistic compounding of paecilomyces lilacinus, pochonia chlamydosporia, trichoderma harzianum and bacillus subtilis is utilized to realize the comprehensive prevention and control of root-knot nematode and soil-borne fungal diseases, and has the functions of nutrient slow release and crop resistance induction.

The invention relates to the technical field of microbial fertilizer preparation, and provides a coating method for improving the coating rate of microbial fertilizer particles in order to solve the problem of poor affinity between a hydrophilic material and hydrophobic microbial fertilizer particles and improve the coating rate of the hydrophilic material as a film layer to the microbial fertilizer particles. The fertilizer is composed of a microbial fertilizer particle core layer and a modified film layer coating the outer layer of the fertilizer, and the mass ratio of the core layer to the film layer is 100: (0.5-10). Wherein the core layer can be made of trichoderma harzianum, penicillium baylii or aspergillus niger microbial fertilizer particles; the raw material of the film layer can be composed of the following components: polyvinyl alcohol (PVA), a hydrophilic material (such as starch, glucose, maltose or dextrin) capable of providing glycogen, a surfactant capable of changing the surface tension of water, a wetting agent, a dispersing agent (such as lauryl sodium sulfate, disodium methylene dinaphthalene sulfonate, sodium dodecyl benzene sulfonate or fatty acid salt) and the like. According to the coating method for improving the coating rate of the microbial fertilizer particles, the higher coating rate is achieved, the coated microbial fertilizer further has the longer container life and the higher pressure resistance, the looseness problem is greatly solved, the problems that traditional microbial fertilizer particles are prone to inactivation, breaking and loosening are solved, and the yield of the microbial fertilizer particles is improved. Good popularization and application values are realized.

The invention provides a DSE chlamydospore water aqua as well as a preparation method and application thereof, and belongs to the technical field of microbial preparations, the DSE chlamydospore water aqua comprises chlamydospore from at least one dark partitioned endophytic fungus, and further comprises 5g / mL-10g / mL of preservative and 5g / mL-10g / mL of protective agent, and the content of the chlamydospore is greater than 2.5 * 10 < 8 > CFU / mL. The product property of the water aqua is improved by reasonably optimizing the dosage of DSE chlamydospore and auxiliary materials, the content of chlamydospore in the obtained water aqua finished product can reach 2.54 * 10 < 8 > CFU / mL, and all indexes meet the national standard; the conidium water aqua can be stored for more than 12 months at normal temperature, and the shelf life is prolonged by more than 9 months compared with a conventional conidium water aqua. The DSE chlamydospore aqueous solution can effectively prevent and treat tomato bacterial wilt and southern rice black-streaked dwarf disease, improve the growth vigor of tomato plants and rice plants, and achieve a prevention and treatment effect which is not inferior to or even superior to that of positive control trichoderma harzianum. The DSE chlamydospore aqueous solution provided by the invention is simple to prepare, controllable in cost and excellent in performance, and is suitable for large-scale popularization and application as a novel biopesticide product.

Disclosed in this disclosure are Acid Sulfate Soil (ASS) improvement materials, and preparation methods and application methods thereof. ASS improvement materials include mixed particle improvement materials and microbial improvement materials. The mixed particle improvement materials include natural mineral materials, organic carbon materials, lignosulfonates, silicates, high molecular polymers, shell powder, fish bone powder, and hydrated lime powder; and the microbial improvement materials include Bacillus megaterium, Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Trichoderma harzianum, yeast, and photosynthetic bacteria.During use, the mixed particle improvement material is evenly distributed in the soil, and is well mixed with the soil through rotary tillage; the microbial improvement material is dissolved in water and added to the irrigated land, the water surface depth is 5-10 cm, the land is continuously soaked for 5-7 days, and then agricultural cultivation activities are carried out after the water level naturally decreases. The ASS improvement material in the present disclosure has the effects of deacidification, maintenance and improvement of fertilizer, and increase in production, and has the characteristics of efficiency, green, environmental friendliness, etc.

The invention belongs to the field of microbial agents, and particularly provides an agricultural microbial agent and a preparation method thereof.The preparation method includes the steps that S01, bacillus subtilis fermentation liquor, trichoderma harzianum spore suspension liquid and rhodopseudomonas palustris fermentation liquor are mixed to obtain compound bacteria liquid; s02, mixing the compound bacterial liquid with the composite carrier powder, oscillating and adsorbing to obtain a wet-state bacterium-loaded complex; s03, mixing the wet bacterium-loaded complex with a freeze-drying protective additive, performing freeze-drying and crushing, and sieving with a 60-mesh sieve to obtain freeze-dried particles; and S04, carrying out spray granulation on the freeze-dried granules by using a coating solution, and then sieving by using a 40-mesh sieve to obtain the microbial agent. The microbial agent prepared in the invention has strong stability to saline-alkali environment, and can play a role for a long time.

This invention discloses a Trichoderma harzianum JT3 and its applications, relating to the field of microbial technology. The aforementioned Trichoderma harzianum ( Trichoderma harzianum JT3, deposited on September 22, 2025, at the China Center for Type Culture Collection (CCTCC), accession number: CCTCC NO: M 20252082, address: Wuhan University, Wuhan, China. This invention also provides applications of *Trichoderma harzianum* JT3. The *Trichoderma harzianum* JT3 strain provided by this invention can produce various lignocellulose-degrading enzymes, efficiently degrading lignin, cellulose, and hemicellulose in agricultural waste such as straw and producing ethyl styrosinate. Simultaneously, it exhibits significant inhibitory effects on various plant pathogens, effectively solving the problems of environmental pollution caused by the difficult degradation of lignin in straw, the decline of ancient trees due to wood-rotting fungi, and the over-reliance on chemical pesticides for plant disease control.

The application discloses a strain of Trichoderma harzianum HMS1 and application of the strain in degradation of low-grade lignite, and belongs to the technical field of microorganisms. The strain is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 42173. The strain has heat resistance, the optimal growth temperature is 35-38 DEG C, and the highest growth temperature is 44 DEG C, so that the strain is suitable for high-temperature environment or reactor operation, and cooling energy consumption is significantly reduced. The strain can directly use untreated low-grade lignite as the only carbon source, and the final degradation rate reaches 49+ / -0.5% through solid culture by means of a plate method for 21 days, the final degradation rate reaches 50.4+ / -0.8% through liquid culture by means of a shaking flask method for 7 days, and the final degradation rate reaches 51.3+ / -1.2% through liquid culture by means of a shaking flask method for 14 days. The strain overcomes the problem that the activity of the existing mesophilic strain is significantly reduced or even inactivated in a high-temperature environment (greater than 35 DEG C), and is particularly suitable for lignite biotransformation treatment and high-temperature composting in a hot and humid area (such as Yunnan, the eastern part of Inner Mongolia, etc. in China) where lignite resources are rich.

The invention relates to the technical field of special fertilizer effect compositions, and particularly discloses a seedling raising and plant promoting composition for mountain mangoes and a rapid production and field planting method. The composition comprises various components such as microbial metabolite fermentation liquor dry powder, trichoderma fermentation filter residue powder, tea bran residue crushed matter, perlite loaded chitosan oligosaccharide composite particles, a kaolin-sepiolite compound and sodium alginate gel particles, and the core components are matched and coordinated to construct a stable seedling environment structure. The invention further provides a rapid production and field planting method matched with the composition, the whole process of seedling raising, bag transferring, shaping and management before outplanting is covered, and the method is suitable for intensive cultivation and efficient transplanting of mangoes under complex mountain conditions and has good technical adaptability and application prospects.

The invention relates to the technical field of seed coating, in particular to a coating agent and a preparation method thereof. The coating agent comprises compound microorganisms, an antibacterial enhancer, a film-forming agent and a dispersing agent; the compound microorganisms comprise azospirillum, pseudomonas fluorescens, bacillus mucilaginosus and trichoderma harzianum; the antibacterial reinforcing agent is prepared from chitosan and epsilon-polylysine; the mass ratio of the compound microorganisms to the antibacterial enhancer is (1-3): (0.5-1). According to the coating agent and the preparation method thereof provided by the invention, the double effects of seed storage and preservation and growth promotion can be taken into account, and meanwhile, the environmental adaptability can be further improved.

This invention discloses a method for preparing bio-organic fertilizer that reduces the incidence of tobacco root-knot nematodes. The bio-organic fertilizer consists of a fermentation substrate, microbial inoculant, and earthworm castings in a mass ratio of 10:0.2-0.4:2-3. The invention uses Bacillus subtilis, Trichoderma harzianum, and Bacillus mucilaginosus in a mass ratio of 15-25:8-13:30-40 to prepare the microbial inoculant. The fermentation substrate and microbial inoculant are mixed and fermented. The fermentation substrate includes cow dung, wheat bran, straw, and earthworm castings. The bio-organic fertilizer of this invention can reduce the incidence of tobacco root-knot nematodes and also promotes tobacco root growth and increases tobacco leaf yield.

This invention relates to the field of microbial technology, specifically to the application of a microbial strain for remediating soil pollution. The strain used in this invention is *Trichoderma harzianum* 124D, which is not only a broad-spectrum biocontrol agent but also significantly reduces the accumulation of copper in the soil, alleviating the stress on wheat growth.

The present application relates to the field of bio-agriculture, and particularly relates to a bio-agricultural compound microbial fertilizer and a preparation method thereof, which comprises, by weight, 2-8 parts of functional microbial flora, 20-48 parts of organic carrier, 5-20 parts of nutrient component, 0.5-4 parts of microbial body protective agent, and the balance of water; the functional microbial flora is composed of Pseudomonas fluorescens, Trichoderma harzianum, Saccharomyces cerevisiae and Bacillus mycoides; and the organic carrier is composed of a peanut shell-sugarcane residue mixture, modified diatomite and humic acid. The present application forms a complete compound microbial fertilizer technical solution through scientific compounding of multiple strains, directional modification of the organic carrier, balanced optimization of the nutrient component and low-temperature preparation process synergy, and has multiple outstanding beneficial effects.

This invention relates to the field of soil improvement technology and discloses a microbial soil remediation material for resisting peanut continuous cropping obstacles and its preparation method. The method includes: pressing sodium alginate solution and Bacillus subtilis or Trichoderma harzianum into the pores of acid-heat activated diatomaceous earth under negative pressure; spraying an acidic chitosan solution under stirring to form a primary coating layer; spraying an alkaline potassium humate solution in a fluidized bed, controlling the wet-bulb temperature at 45-55℃, and the dry mass ratio of humic acid to chitosan at 1.8:1-2.4:1, inducing the directional migration of hydrophobic groups in humic acid and causing in-situ complexation reactions; and performing deep dehydration and solidification. This invention constructs an asymmetric shell structure with a hydrophobic outer surface and retained hydrophilic ion transport channels internally, which can resist soil moisture erosion, allow small molecule phenolic acids to permeate for microbial degradation, and simultaneously retain large molecule pathogens, achieving targeted degradation of continuous cropping toxins and efficient colonization of functional bacteria.

U I T T R E K S E L The present invention belongs to the field of agricultural microbial control technology, and specifically relates to a growth—promoting and disease—preventing compound microbial inoculant, a preparation method and an application thereof. The compound microbial inoculant specifically includes microbial powder, growth auxiliary materials, and. a desiccant. The microorganisms include Talaromyces flavus, Trichoderma harzianum, Bacillus velezensis, Bacillus licheniformis, and Brevibacillus laterosporus. Through the synergistic effect of multiple microbial strains, the compound microbial inoculant of the present invention enhances the control effect against Verticillium wilt pathogens, and overcomes the shortcomings of a single biocontrol microbe, such as a single biocontrol mechanism, poor environmental adaptability, inability to colonize in soil for a long time, and unstable control effect. Furthermore, the compound microbial inoculant of the present invention can increase the yields of cotton and pepper, and improve the fiber quality of cotton. The present invention provides scientific and technological support and decision-making services for realizing green, environmentally friendly, cost—saving, and efficiency-increasing modern agriculture.

This invention relates to the field of agricultural biological control technology, specifically to a synthetic microbial co-culture fermentation broth, its inoculum, and its application. The synthetic microbial co-culture fermentation broth is prepared by sequential co-inoculation fermentation of *Trichoderma harzianum* TW21990, *Burkholderia viminalis* P418, and *Bacillus atrophicus* BsR05; wherein *Trichoderma harzianum* TW21990 has the CGMCC No. 12864, *Burkholderia viminalis* P418 has the CGMCC No. 1212, and *Bacillus atrophicus* BsR05 has the CGMCC No. 11665. Compared to single biocontrol strains, the antibacterial and growth-promoting secondary metabolites produced by the above-mentioned synthetic microbial co-culture fermentation are broad-spectrum, making it less likely to induce drug resistance in the control of gray mold in leeks. The control effect is stable, there is no antagonism within the synthetic microbial community, and the efficacy of the strains is synergistically enhanced, with a significant growth-promoting effect on leeks.