207 results about "Trichoderma harzianum" patented technology

This invention discloses a microbial inoculant for controlling peanut root rot and its preparation method, belonging to the field of microbial materials technology. This invention screened and obtained a *Trichoderma harzianum* strain with highly efficient inhibitory effects against the pathogen causing peanut root rot. Firstly, the strain itself exhibits antagonistic activity against the peanut pathogen. Secondly, it was prepared as a spore suspension and fermented in solid-state with rice husks and wheat bran. During the solid-state fermentation process, the metabolites produced by *Trichoderma harzianum* promote the growth and development of plant roots, increasing plant biomass and yield. This microbial inoculant effectively controls peanut root rot, exhibiting good inhibitory effects against the pathogen, thus improving peanut quality and disease resistance. Furthermore, it improves soil ecological activity to a certain extent, making it worthy of application and promotion.

The invention provides a microbial complex microbial inoculant and application thereof in prevention and treatment of plant diseases, the microbial complex microbial inoculant comprises the following strains by mass: 30%-50% of bacillus subtilis; 20%-40% of bacillus amyloliquefaciens; 10%-30% of pseudomonas fluorescens; 5%-15% of trichoderma harzianum; and 2%-5% of an auxiliary agent. According to the microbial complex microbial inoculant disclosed by the invention, a multiple prevention and control mechanism of'bacteriostasis-competition-parasitism 'is constructed through the synergistic effect of various strains, and meanwhile, the double-layer effect of thallus protection and function enhancement is realized through the assistance of the auxiliaries, so that the prevention and control effect of the prepared microbial complex microbial inoculant is greatly improved.

The invention belongs to the technical field of coastal saline-alkali soil remediation, and particularly relates to a composite microbial agent for coastal saline-alkali soil remediation. The microbial agent is in a granule form, and comprises a carrier, which is a mixture of straw powder and duck manure; the active flora comprises bacillus licheniformis, bacillus megaterium, brevibacillus laterosporus, trichoderma harzianum spore powder, paenibacillus polymyxa, halomonas and pseudomonas fluorescens, the active flora is adsorbed in the carrier, and the halomonas and the pseudomonas fluorescens are subjected to embedding treatment; and the spraying film is sprayed on the surface of the carrier. Through the synergistic effect of multiple strains, the improvement effect on saline-alkali soil and the growth promoting effect on crop growth are improved, and the defect that the function of a single strain is limited is overcome.

The invention discloses dsRNA of a targeted BdSpt7 gene, a biocontrol agent and application of the dsRNA. The sequence of the dsRNA of the targeted BdSpt7 gene is as shown in SEQ ID No.2. The invention further discloses a preparation method of the biocontrol agent of the targeted BdSpt7 gene. The active ingredient of the biocontrol agent is the dsRNA of the target BdSpt7 gene. The invention also provides an application of the botryosphaeria dothidea BdSpt7 gene in prevention and treatment of apple botryosphaeria dothidea, and a nucleotide sequence of the botryosphaeria dothidea BdSpt7 gene is shown as SEQ ID No.1. The invention also provides an African trichoderma harzianum engineering strain for expressing dsRNA, the African trichoderma harzianum engineering strain is integrated with a dsRNA expression box for interfering gene expression of a target gene BdSpt7, the dsRNA expression box is shown as SEQ ID No.1, and the African trichoderma harzianum is an African trichoderma harzianum with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 41709. The dsRNA targeting the BdSpt7 gene can significantly reduce the pathogenicity of the physalospora piricola through interference, and the African trichoderma harzianum engineering strain can colonize on the plant surface and continuously express the dsRNA so as to play a role in preventing and treating the physalospora piricola, and is green and environment-friendly.

The invention belongs to the technical field of agricultural microbial control, and particularly relates to a growth-promoting and disease-preventing compound microbial agent and a preparation method and application thereof, and the compound microbial agent specifically comprises microbial powder, a growth auxiliary material and a drying agent; the microorganisms comprise vermiform yellow, trichoderma harzianum, bacillus velezensis, bacillus licheniformis and brevibacillus laterosporus. According to the compound microbial agent, through the synergistic effect of multiple strains, the control effect of the compound microbial agent on verticillium dahliae is enhanced, and the defects that a single biocontrol bacterium is single in biocontrol mechanism, poor in environment adaptability, incapable of being colonized in soil for a long time and unstable in control effect are overcome; the compound microbial agent can increase the yield of cotton and pepper and improve the quality of cotton fibers, and provides scientific and technological support and decision service for realizing green, environment-friendly, cost-saving and efficiency-increasing modern agriculture.

The invention discloses a trans-boundary synthetic flora (SMC) for preventing and treating wheat stem rot as well as preparation and application of the trans-boundary synthetic flora. The synthetic bacterial flora is prepared by compounding Trichoderma harzianum T19 with the preservation number of CCTCC (China Center for Type Culture Collection) No: M 20251896, bacillus subtilis BS-Z15 with the preservation number of CCTCC No: M 20251892, two strains of growth promoting bacillus PM9 and PM16, and two strains of auxiliary bacillus S8 and S32 according to a specific proportion. Compared with an existing single antagonistic bacterial agent, the synthetic flora disclosed by the invention has the advantages that the bacteriostasis rate of the synthetic flora on Fusarium pseudograminearum under a non-sterile soil condition can reach 70% or above, and the disease index is reduced by 70.01%; meanwhile, the plant height of wheat is increased by 28.63%, the root length is increased by 24.53%, the fresh weight is increased by 181.21%, the dry weight is increased by 184.64%, the thousand seed weight is increased by 10.39%, and the rhizosphere microbial community structure and the soil enzyme activity are remarkably improved. The method can be used for green prevention and control and yield increase of basal stem rot of wheat and other gramineous crops.

The invention discloses a Trichoderma harzianum-containing biocontrol fertilizer and a preparation method thereof, and belongs to the technical field of mixed fertilizers, the fertilizer comprises composite matrix particles (containing an organic carrier, an inorganic fertilizer and trace elements), a microsphere coating layer (composed of sodium alginate / chitosan gel microspheres) coated with Trichoderma harzianum on the surface, and a water-soluble fertilizer coating layer (composed of sodium alginate / chitosan gel microspheres) coated with Trichoderma harzianum on the surface, and the outermost layer is a biodegradable protective film (modified starch / PVA / PLA). The preparation method comprises the following steps: granulating a matrix, preparing a bacterial suspension, forming bacteria-containing gel microspheres (the spore recovery rate is greater than or equal to 95%), attaching the microspheres to the matrix, and coating the outer layer. According to the structure, the survival rate of viable bacteria stored at room temperature for 120 days is larger than or equal to 68.3%, the rhizosphere bacterial count is larger than or equal to 4.58 * 10 < 6 > CFU / g soil after the viable bacteria are applied to the soil for 30 days, soil-borne diseases are remarkably inhibited, and the crop yield is increased by 13.5% (cucumber yield is increased by 13.5%).

The invention provides a compound microbial agent capable of resisting diseases and insect pests and a preparation method thereof, and the compound microbial agent consists of the following substances in parts by mass: 50-100 parts of bacillus subtilis, 40-80 parts of beauveria bassiana, 35-75 parts of trichoderma harzianum, 20-50 parts of modified straw powder, 10-15 parts of a humic acid-diatomite complex, 10-15 parts of trehalose, 5-10 parts of chitin powder and 5-10 parts of shellac. According to the invention, the modified straw powder and the humic acid-diatomite complex are adopted as a core carrier, so that the defects of poor adsorbability and nutrition deficiency of the traditional diatomite or synthetic carrier are overcome, and a high-porosity and nutrient-rich living environment is provided for bacillus subtilis, beauveria bassiana and trichoderma harzianum; compared with the prior art, the method provided by the invention has the advantages that the cost is low, the field planting rate and the field survival rate of viable bacteria in the microbial inoculum are remarkably improved, meanwhile, controllable coating is performed by utilizing an ethanol solution of shellac, a refractory synthetic polymer coating material is replaced, thalli are protected from environmental stress, and meanwhile, the risk of lasting residual pollution of a chemical coating material in soil is avoided.

The invention relates to the technical field of cyanobacterial bloom control in water, and particularly discloses a compound enzyme-bacterium synergistic preparation for cyanobacterial bloom control, a preparation method and a use method. The preparation comprises the following components in percentage by mass: compound enzymes (10-20% of cellulase, 8-12% of beta-1, 3-glucanase and 3-8% of lipase), functional bacteria (10-20% of bacillus subtilis, 8-15% of trichoderma harzianum and 5-10% of bacillus amyloliquefaciens), an enzyme stabilizer (3-8% of trehalose), an adsorption carrier (15-25% of Fe < 3 + > modified charcoal and 8-15% of diatomite) and auxiliary components. The preparation method comprises the steps of carrier modification, solid fermentation and microcapsule embedding. During use, 3-20kg / 1000m < 3 > of water is added for the first time, 2-10kg / 1000m < 3 > of water is added every 7-14 days in the maintenance stage, and synchronous aeration is performed. Through the synergistic effect of beta-1, 3-glucanase and trichoderma harzianum, the algicidal rate is 92%, and the spore germination inhibition rate is 82%; the Fe < 3 + > modified charcoal improves the stability of the enzyme in an alkaline environment (80% of activity is retained when the pH value is 9); the method is suitable for eutrophic water with chlorophyll a greater than or equal to 30 [mu] g / L, and has no chemical residue risk.

The invention belongs to the technical field of soil improvement, and discloses a preparation method of a composite microbial agent and application of the composite microbial agent in continuous cropping stress resistance, the active ingredients of the composite microbial agent comprise Gliocladium mucilaginosum, Trichoderma harzianum and Pseudomonas chlororaphis, and a carrier contains a basic carrier, a synergistic component and a soil adaptive component (oyster shell powder or wormcast particles). The preparation method comprises the following steps: strain fermentation, carrier construction, solid-state co-fermentation, and mixing of a dried fermentation product and a carrier. After the compound microbial agent is used, the abundance of gliocladium mucilaginosum, trichoderma harzianum and pseudomonas chlororaphis in continuous cropping crop soil is obviously increased, and the abundance of fusarium oxysporum is obviously reduced; the acidification condition of the acidified soil is obviously improved, the content of organic acids (ferulic acid and vanillic acid) in the soil is obviously reduced, and the soil becomes loose from hardening, so that the root activity is improved, and the plant diseases and insect pests are resisted; the single fruit weight and sugar degree can be remarkably improved, the fruit storage rotting rate is reduced, and the effect is remarkable.

The invention discloses a modifier for reducing wine grape soil obstacles and a preparation method and application thereof, and belongs to the technical field of soil improvement. According to the invention, the specific complex microbial inoculant composed of bacillus subtilis, bacillus licheniformis and trichoderma harzianum is adopted to ferment the grape pomace, antibacterial substances and organic acids can be generated, and saline-alkaline obstacles of soil can be relieved; the fermentation product contains a large amount of bacillus subtilis, bacillus licheniformis and trichoderma harzianum, and can occupy the ecological niche of the root system through competition, optimize the rhizosphere environment and improve the biological enzyme activity of soil. Through the synergistic effect with the decomposed straw, the humic acid and the furfural residues, the rhizosphere microenvironment can be remarkably improved, decomposition and nutrient conversion of organic substances in soil are accelerated, the soil fertility is improved, root systems can better extend and absorb nutrients, plant growth is promoted, and the yield and quality of fruits are improved.

The invention discloses a preparation method and application of an anti-continuous cropping microbial fertilizer, and aims to solve the problems of aggravation of crop diseases and insect pests and reduction of yield and quality caused by continuous cropping planting. The preparation method comprises the following steps: activating and uniformly mixing bacillus subtilis and bacillus licheniformis, and adding an alpha-lactalbumin solution and a calcium chloride solution to prepare a bacillus aggregate; adding bentonite into the polytrehalose solution, and performing ultrasonic treatment on the mixture and the humic acid solution to prepare a modified polytrehalose-bentonite compound; uniformly mixing acremonium terricola, trichoderma harzianum and a bacillus aggregate, and adding a wall material solution to prepare a compound bacterium microcapsule preparation; and finally, mixing the microcapsules with the corn straw biochar, the chitosan oligosaccharide and the like to obtain a finished product. Through calcium ion induction and humic acid modified embedding, the strain stability and viable count are improved, soil organic matter and nitrogen phosphorus and potassium nutrients can be activated, pathogenic bacteria are inhibited, the crop rhizosphere environment is improved, the growth index and quality of red kidney beans and potatoes are effectively improved, and the method is suitable for continuous cropping resistant planting of crops.

The invention provides a trichoderma fungicide for promoting growth of allium plants and application of the trichoderma fungicide, the trichoderma fungicide is a trichoderma harzianum strain MCBC3, the trichoderma fungicide and diammonium phosphate are applied in a combined mode, the application amount of the diammonium phosphate is 15 kg / mu, and the application amount of the trichoderma fungicide is 1 L / mu; existing trichoderma resource development focuses on a normal soil environment, the trichoderma harzianum MCBC3 is screened out from the saline-alkali soil for the first time, the salt resistance and the alkali resistance of the trichoderma harzianum MCBC3 directly aim at double stress of high salt and high alkali of the saline-alkali soil, and the technical bottlenecks that a traditional strain is poor in adaptability and low in survival rate in an extreme environment are solved.

The invention discloses a method for carrying out soil treatment by using ardealite and a microbial agent, and aims to solve the problem that ardealite as an industrial by-product is difficult to recycle. According to the method, 5% of a microbial agent (one of trichoderma harzianum, arbuscular mycorrhizal fungi or bacillus laterosporus) and 5%-15% of biochar are applied to phosphogypsum in a combined mode, the nutrient content of a phosphogypsum matrix is remarkably increased, and meanwhile specific pollutants (such as soluble phosphorus, fluorine and heavy metal) are stabilized and solidified. Experimental results show that the technology can effectively regulate and control the pH value of the phosphogypsum matrix, obviously increase the diversity of effective microbial communities and improve the micro-ecological environment of the phosphogypsum matrix. The ardealite combined with the microbial agent and the biochar can be used as a plant growth matrix or a soil substitute material for ecological restoration of mines, has the characteristics of low cost and high efficiency, and provides a feasible way for harmlessness and soil utilization of the ardealite.

The invention discloses a trichoderma complex microbial inoculant capable of promoting wheat growth and preventing and treating wheat stem rot and application of the trichoderma complex microbial inoculant. The trichoderma complex microbial inoculant comprises a trichoderma asperellum strain XX-57 and a trichoderma harzianum strain PY-127, and the preservation number of the trichoderma asperellum strain XX-57 is CGMCC No. 41937; the preservation number of the trichoderma harzianum strain PY-127 is CGMCC (China General Microbiological Culture Collection Center) NO. 41938. The invention further specifically discloses application of the trichoderma complex microbial inoculant in promoting wheat seed germination and wheat growth and preventing and treating wheat basal stem rot, the trichoderma complex microbial inoculant is applied 10-20 days after wheat seedling emergence, and it is found that compared with single trichoderma inoculation, the trichoderma complex microbial inoculant has the promoting effect on wheat plant height, root length, SPAD value, fresh weight and dry weight; the control effect on wheat stem rot is better, and particularly the control effect is more obvious when pathogenic bacteria are inoculated in advance.

This invention relates to the field of fertilizer technology, specifically to a disease-resistant microbial compound fertilizer and its preparation method. The disease-resistant microbial compound fertilizer comprises the following raw materials in parts by weight: 10-15 parts nitrogen fertilizer, 10-20 parts phosphorus fertilizer, 6-10 parts potassium fertilizer, 50-70 parts fermented organic matter, 8-12 parts fertilizer efficiency enhancer, 5-8 parts disease-resistant composition, and 6-10 parts chitosan oligosaccharide. The disease-resistant composition is a mixture of sodium selenite and potassium humate in a weight ratio of 1:1.5-2.5. The microbial compound fertilizer prepared by this invention not only achieves a combination of rapid and long-lasting effects, improving fertilizer utilization, but also increases organic matter, activates fixed phosphorus and potassium elements in the soil, and improves the micro-ecological environment. Furthermore, through the synergistic effect of Trichoderma harzianum inoculum, Paecilomyces lilacinus, the disease-resistant composition, and chitosan oligosaccharide, it exhibits good inhibitory effects on soil-borne diseases and some foliar diseases, promoting robust crop growth and improving both crop yield and quality.

The invention discloses an application of trichoderma harzianum in biological prevention and treatment of wild apple tree canker, the classification name of the trichoderma harzianum is Bacillus velezensis Rifai, the preservation unit is China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO. 42231, and the preservation time is September 29, 2025. Experimental results show that the trichoderma harzianum has remarkable antagonistic activity on parasitic cyst, apple cyst, nutshell cyst, cyst hormaechei, prunus cyst and pear cyst, is environment-friendly and pollution-free, and also provides a safe and reliable new way for preventing and treating the valsa mali of the wild apples; in addition, the research also finds that the fermentation product of the trichoderma harzianum has a certain biocontrol effect on the in-vitro branches infected by the physosporium piricola, so that the trichoderma harzianum and the fermentation product of the trichoderma harzianum provided by the invention can be used for preventing and treating the wild apple tree canker, and have good development and application prospects.

The application discloses a kind of fermentation complex microbial inoculant and preparation method and application thereof, the fermentation complex microbial inoculant includes the following weight parts of component: bacillus subtilis microbial inoculant 2-5 parts, bacillus mucilaginosus microbial inoculant 5-10 parts, yeast microbial inoculant 2-10 parts, trichoderma harzianum microbial inoculant 10-20 parts, aspergillus niger microbial inoculant 10-20 parts and streptococcus thermophilus microbial inoculant 2-5 parts;The fermentation complex microbial inoculant of the application is prepared by selecting each strain and amount, is added to pigeon manure and is fermented to prepare organic fertilizer, can better decompose macromolecular substance, significantly improve the organic matter and total nitrogen content in preparation organic fertilizer, and can better improve seed germination rate;In addition, the preparation method of pigeon manure organic fertilizer of the application is simple in process, easy to operate, and convenient for production.

The invention discloses a bio-organic fertilizer and application thereof, belongs to the field of bio-organic fertilizers, and solves the problem of environmental pollution caused by stacking or landfill of potato residues. The bio-organic fertilizer comprises potato residue zymolyte, a decomposed organic fertilizer and a compound microbial agent, the compound microbial agent is prepared from trichoderma harzianum, bacillus subtilis and bacillus velezensis; the preparation method comprises the following steps: mixing the potato residue zymolyte, the decomposed organic fertilizer and the compound microbial agent, controlling the water content to be 40-50%, fermenting at 40-50 DEG C for 10-15 days, and airing and crushing a fermented product to obtain a finished product. The invention relates to application of a bio-organic fertilizer in quality improvement and yield increase of crops. According to the method, the potato residues which are difficult to degrade are converted into organic fertilizers with high added values through a directional enzymolysis technology, so that the problem of environmental pollution is solved; the compound microbial flora and the enzymolysis product have a synergistic effect to accelerate the conversion of organic matters; the fertilizer is rich in active organic matters and functional microorganisms, and can significantly improve the soil structure.

The invention discloses a microbial composition for preventing and treating vomitoxin as well as a preparation method and application of the microbial composition. The microbial composition for preventing and treating vomitoxin is mainly prepared by mixing a component I and a component II according to a mass ratio of (50.0-0.1): (50.0-99.9), the component I comprises trichoderma harzianum powder, trichoderma asperellum powder and penicillium bayi powder; and the component II comprises potassium phosphite, alkene potassium phosphate and potassium tripolyphosphate. The preparation method comprises the following steps: adding the component I and the component II into a stirrer, and stirring and mixing to obtain the coating. The invention also discloses an application of the microbial composition for preventing and treating vomitoxin. The microbial composition disclosed by the invention can generate metabolites with a biocontrol effect, has efficient inhibitory activity on fusarium, can reduce generation of vomitoxin, can effectively regulate diversity of soil microorganisms, realizes crop yield increase and soil improvement, and is green and environment-friendly. The method and application are simple, the cost is low, and the method is suitable for agricultural application.

Carrier-based agricultural biofertilizer composition comprising an isolated Trichoderma harzianum CGAJ2T-3 of deposit accession number KY495199 and an agriculturally acceptable formulation comprising a stabilizer (e.g., carboxymethyl cellulose (CMC)). The strain may be isolated from the vegetable rhizosphere and rhizoplane from Yola, Nigeria; e.g., by serial dilution and spread plate method. The formulation may comprise carriers selected from: talc, vermiculite, peat, compost. Also disclosed method for preparing carrier-based agricultural biofertilizer composition, comprising: preparing nutrient medium comprising carbon and nitrogen sources (e.g., sucrose, glucose, malt extract, peptone); inoculating medium with a pure culture of Trichoderma harzianum CGAJ2T-3; performing fermentation; extracting fungal spores and mycelia from the broth (e.g., filtration, centrifugation); employing a solid substrate fermentation technique (e.g., utilising rice bran and wheat straw) to attain 3.0×10^9 CFU / g; diluting formulation to 3.0×10^9 CFU / mL within talc powder-based or liquid formulation; and administering formulation to soil (e.g., spraying onto foliage or root zones).

The invention belongs to the technical field of biological phosphorus-silicon fertilizers, and particularly relates to a liquid complex microbial inoculant capable of repairing heavy metals and activating phosphorus and silicon and a biological phosphorus-silicon fertilizer thereof. The liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria Cupriavidus and Ochrobactrum, nitrogen-fixing bacteria Azospirillum brasilense, phosphate solubilizing bacteria Bacillus megaterium and Pseudomonas, potassium solubilizing bacteria Bacillus mucilaginosus, growth-promoting bacteria Pseudomonas fluorescens, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants and microbial inoculants. The biocontrol bacteria are trichoderma harzianum and bacillus subtilis, and the synergistic bacteria are saccharomyces cerevisiae; the biological phosphorus-silicon fertilizer is prepared from the liquid complex microbial inoculant. According to the biological phosphorus-silicon fertilizer provided by the invention, through scientific compatibility of various functional strains, heavy metal remediation and soil phosphorus and silicon solubilization are realized, absorption of phosphorus and silicon in soil is promoted, and the crop yield is increased.

The application provides a Rhodiola kirilowii root rot harmless prevention and control method and relates to the technical field of plant disease prevention and control. The Rhodiola kirilowii root rot harmless prevention and control method is characterized by the following steps: after seed disinfection, the seed is treated by immersion in a solution of a Yunnan sclerotia of Sclerotinia spore powder extract, and a fermentation substrate of Bacillus subtilis and Bacillus amyloliquefaciens is used, and then the roots are irrigated with a solution of a Yunnan sclerotia of Sclerotinia spore powder extract and a Trichoderma harzianum liquid. The method can effectively prevent and control the Rhodiola kirilowii root rot, reduce the use of chemical pesticides, reduce pesticide residues, and improve the safety of planting.

The invention discloses a Chinese angelica out-of-season single seedling raising method suitable for mechanical transplanting and application thereof, and belongs to the field of Chinese herbal medicine seedling raising and cultivation. According to the method, a sunlight greenhouse or a gutter-connected greenhouse with the altitude being 2300 m or above and the lowest temperature being larger than or equal to 8 DEG C in winter is selected, field soil without continuous cropping obstacles is matched with fried rapeseeds, trichoderma harzianum and bacillus subtilis to prepare a special matrix, a seedling raising paper book with the single-hole diameter being 19-25 mm and the height being 12 cm is adopted, pelletized seeds are dibbled in a double-grain mode from late November to early December, and the seeds are sown in the seedling raising paper book with the single-hole diameter being 19-25 mm and the height being 12 cm. Through management of precise temperature and humidity regulation and control, foliage dressing, seedling hardening and leaf cutting and the like, standardized single seedlings are cultivated and transplanted through a transplanter. According to the method, the early bolting rate of the angelica sinensis is reduced, the seedling uniformity and the transplanting survival rate are improved, the transplanting efficiency is improved, the labor cost is reduced, the yield of the angelica sinensis can be increased, efficient utilization of out-of-season resources is achieved, and support is provided for industrial large-scale standardized development.

The invention discloses a microbial compound fermentation inoculant, a straw fermentation product and application of the straw fermentation product, and belongs to the technical field of agricultural microorganisms. The preparation method comprises the following steps: compounding bacillus subtilis, trichoderma harzianum and trichoderma longibrachiatum to form a microbial compound fermentation inoculant; when the microbial compound fermentation inoculant is used for fermenting ginger straws, the content of pyrogallic acid in a prepared straw fermentation product can be synergistically improved. The pyrogallic acid has an obvious antibacterial effect on fusarium moniliforme and fusarium laminatum which cause the apple continuous cropping obstacle; the growth of apple seedlings planted in continuous cropping soil can be effectively promoted. Therefore, the straw fermentation product disclosed by the invention can be used for preventing and treating apple continuous cropping obstacles, improving the biomass and root activity of apple seedlings under continuous cropping conditions and reducing the quantity of harmful fusarium in continuous cropping soil.

This invention belongs to the field of gene technology for disease control. This invention provides a method for preparing biocontrol engineered bacteria using *Trichoderma harzianum* as a dsRNA vector, comprising the following steps: determining the target gene based on the pathogenic fungus; using the target gene as an arm sequence, and centering on the VdTublin intron sequence of the *Verticillium dahliae* endogenous gene, connecting two identical arm sequences to both ends of the intron in a forward and reverse direction, respectively, to construct the target sequence; and transferring the target sequence into *Trichoderma harzianum* using ATMT to obtain engineered *Trichoderma harzianum* bacteria. The engineered strain obtained by this invention has a good inhibitory effect on the growth and pathogenicity of the corresponding fungus.

The invention provides a rapid dormancy breaking method for sand whip seeds and dormancy breaking sand whip seeds, and relates to the technical field of plant seed treatment.The method comprises the following steps that 1, physical initiation treatment is conducted, specifically, the disinfected sand whip seeds are placed in warm water at the temperature of 45-55 DEG C to be soaked for 10-14 h; (2) chemical initiation treatment: soaking the seeds treated in the step (1) in a gibberellin solution with the mass concentration of 0.03%-0.07% for 20-28 hours; (3) biological seed dressing treatment: carrying out seed dressing on the seeds treated in the step (2) and a trichoderma harzianum fungicide, wherein the mass of the fungicide is 0.05-0.15% of the mass of the seeds; according to the method, the dormancy mechanism of the sand penis seeds can be effectively broken, germination of the seeds is promoted, the germination rate of the sand penis seeds treated by the optimized method can reach 87.00% on the 21st day, the germination synchronism of the seeds can be higher, and the problem of irregular natural germination is solved.

The present application relates to the technical field of microorganism, and particularly relates to a microbial composition, a compound microbial agent and application.The microbial composition of the present application comprises Bacillus amyloliquefaciens, Bacillus brevis and Trichoderma harzianum, the microbial composition of the present application can simultaneously prevent and treat rice seed production process rice blast and storage process aspergillus flavus pollution, realize the whole life cycle protection of rice seed from production to storage, solve the problem that different agents need to be used to prevent and treat rice blast and aspergillus flavus pollution in the prior art.In addition, the compound microbial agent containing the microbial composition is safe, efficient, environmentally friendly, can replace chemical pesticides, reduce environmental pollution and pesticide residues, and the preparation method is simple, easy to industrial production, and has wide application prospect.

The invention relates to a microbial inoculum for improving the transplanting survival rate of xanthoceras sorbifolia bunge and a preparation method thereof.The microbial inoculum for improving the transplanting survival rate of xanthoceras sorbifolia bunge is characterized by comprising trichoderma harzianum, yeast cell walls and organic matter, and the organic matter contains monosaccharide or polysaccharide capable of being utilized by the trichoderma harzianum. The transplanting survival rate of xanthoceras sorbifolia bunge can be increased.

The invention belongs to the technical field of biological bacterial fertilizers, and particularly relates to a high-salt-tolerance microbial liquid complex microbial inoculant suitable for saline-alkali desertification land and a biological bacterial fertilizer thereof. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation of three stages: salt-tolerant repair bacteria Nocardia halophila, salt-tolerant functional bacteria Bacillus subtilis and Azospirillum brasilense, nitrogen-fixing functional bacteria Azotobacter chroococcum, phosphorus-solubilizing functional bacteria Pseudomonas fluorescens, and nitrogen-solubilizing functional bacteria Pseudomonas fluorescens. The bacillus mucilaginosus is used as a potassium-dissolving functional bacterium, the trichoderma asperellum is used as a biocontrol growth-promoting bacterium, and the trichoderma harzianum is used as a rhizosphere optimizing bacterium. The biological bacterial fertilizer is prepared from the liquid complex microbial inoculant. Through scientific compatibility of different strains provided by the invention, efficient improvement and crop yield increase of the saline-alkali desertification land are realized, and the strain has important economic and social values.