17 results about "Physalospora" patented technology

The invention discloses an African trichoderma harzianum strain 6-5 and application thereof, the disclosed strain is named as Trichoderma harzianum, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No. 41930. The bacterial strain has obvious bacteriostatic activity on crop pathogenic bacteria such as colletotrichum capsici, apple cyst, botrytis cinerea, alternaria longibrachiata and phytophthora capsici, and fermentation liquor of the bacterial strain has good prevention and control effects on apple anthracnose leaf blight, apple bitter rot and apple tree canker.

The invention discloses dsRNA of a targeted BdSpt7 gene, a biocontrol agent and application of the dsRNA. The sequence of the dsRNA of the targeted BdSpt7 gene is as shown in SEQ ID No.2. The invention further discloses a preparation method of the biocontrol agent of the targeted BdSpt7 gene. The active ingredient of the biocontrol agent is the dsRNA of the target BdSpt7 gene. The invention also provides an application of the botryosphaeria dothidea BdSpt7 gene in prevention and treatment of apple botryosphaeria dothidea, and a nucleotide sequence of the botryosphaeria dothidea BdSpt7 gene is shown as SEQ ID No.1. The invention also provides an African trichoderma harzianum engineering strain for expressing dsRNA, the African trichoderma harzianum engineering strain is integrated with a dsRNA expression box for interfering gene expression of a target gene BdSpt7, the dsRNA expression box is shown as SEQ ID No.1, and the African trichoderma harzianum is an African trichoderma harzianum with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 41709. The dsRNA targeting the BdSpt7 gene can significantly reduce the pathogenicity of the physalospora piricola through interference, and the African trichoderma harzianum engineering strain can colonize on the plant surface and continuously express the dsRNA so as to play a role in preventing and treating the physalospora piricola, and is green and environment-friendly.

The invention relates to the field of microorganisms, in particular to a broad-spectrum antagonistic bacterium bacillus velezensis SJD33 and application thereof. The preservation number of the bacillus velezensis SJD33 provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.29456. The bacillus velezensis SJD33 provided by the invention has the advantages that the preservation number is CGMCC No.29456; the strain has a remarkable inhibition effect on pear fruit rot pathogens (physalospora piricola, penicillium, alternaria alternata, athelia fruit rot pathogens, botrytis cinerea and colletotrichum gloeosporioides) and other fruit pathogenic bacteria (apple core pathogens, brown rot pathogens and pomegranate dry rot pathogens). The SJD33 can obviously reduce the natural rotting rate of the pear fruits; the SJD33 can be rapidly colonized in fruit wounds; the SJD33 does not influence the fruit quality and can delay the reduction of the fruit hardness and the L-ascorbic acid; the SJD33 secondary metabolite is stable in activity and has great application potential. The strain provides a new strain resource for a fruit disease biocontrol resource library.

The invention discloses application of MdDRK1 and MdDRKIP1 genes in improvement of apple ring spot resistance, and belongs to the technical field of gene engineering. According to the application of the MdDRK1 gene and the MdDRKIP1 gene in improving the resistance to the apple ring spot, a CDS sequence for coding the MdDRK1 gene is as shown in SEQ ID NO.7, and a CDS sequence for coding the MdDRKIP1 gene is as shown in SEQ ID NO.15. According to the application of the MdDRK1 gene and the MdDRKIP1 gene in improving the resistance to the apple ring spot. The contents of resistant substances, namely total phenols and total flavonoids, in the overexpression MdDRK1 apple callus and the overexpression MdDRKIP1 apple callus are obviously increased. The research shows that the MdDRK1 and MdDRKIP1 genes play an important role in improving the resistance of apples to the ring spot, have the potential to be applied to gene editing and cultivation of new varieties resistant to the ring spot, and have important significance on directed genetic improvement of the disease resistance of the apples.

The invention discloses application of a KASP molecular marker in identification of resistance to physalospora piricola of tea trees, and belongs to the technical field of plant molecular biology. The invention aims to develop the KASP molecular marker closely linked with the resistance of the tea tree physalospora piricola and accelerate the process of assisted breeding of the molecular marker with the resistance of the tea tree physalospora piricola. The KASP marker for identifying the resistance to the physalospora piricola of the tea tree is Cs-KASP, and the marker is positioned to an SNP related to identification of the resistance to the physalospora piricola of the tea tree through generic genome sequence alignment. A group of KASP molecular markers closely linked with the resistance of the tea tree physalospora piricola are developed according to the SNP site. The marker can be used for genotype detection of different resistant materials of the tea tree physalospora piricola, can be used for successfully distinguishing a disease-resistant material from a disease-susceptible material, and is combined with a high-throughput genotyping detection system to quickly identify physalospora piricola-resistant germplasm resources, so that the breeding efficiency is improved.

The invention relates to the field of tea tree planting methods, in particular to an ecological niche complementary method for interplanting camphor trees with tea trees and application of the ecological niche complementary method. The method has the following effects: 1, interplanting of the cinnamomum camphora and the tea tree has an inhibition phenomenon on growth of physalospora piricola and colletotrichum gloeosporioides; 2, tea polyphenol of tea leaves can be reduced by interplanting the camphor trees and the tea trees; 3, the soil conductivity and the content of organic matters, alkali-hydrolyzable nitrogen and available phosphorus can be improved by intercropping cinnamomum camphora in the tea garden; and 4, tea amino acids can be increased by interplanting the camphor trees and the tea trees.

The invention relates to the technical field of gene engineering, in particular to application of a CsMAPK3 gene in resisting physalospora piricola of tea trees, the base sequence of the CsMAPK3 gene is shown as SEQ ID NO.3, and the resistance of the tea trees to physalospora piricola is increased through overexpression of the CsMAPK3 gene. The regulation and control mechanism of the CsMAPK3 gene disclosed in the invention on the resistance of the tea tree physalospora piricola provides a basis for the subsequent application of the CsMAPK3 gene to the resistance of the tea tree physalospora piricola.

The invention provides a strain of bacillus siamensis Bsiamensis and application thereof, the strain is classified and named as bacillus siamensis Bsiamensis, the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO: 35785, and the preservation date is September 1, 2025. The bacterial suspension, the fermentation liquor and the ethyl acetate extract of the fermentation liquor of the bacterial strain have good bacteriostatic activity on fusarium graminearum, fusarium oxysporum, fusarium laminatum, alternaria longibrachiata, colletotrichum gloeosporioides and colletotrichum frutescens.

The present invention provides a Brevibacillus formosus strain SXM-17, its derivative products and applications in preventing and controlling crop diseases, belonging to the technical field of functional microorganisms. In the present invention, a strain SXM-17 is isolated and purified from wheat plants, and the preservation number is CGMCC No. 30198, which has good inhibitory effects on Gibberella zeae, Physalospora piricola, Botrytis cinerea, Alternaria solani, Rhizoctonia solani and Gaeumannomyces graminis var. tritici, etc. Experiments show that the culture solution, bacterial suspension and supernatant of the strain SXM-17 all have good control effects. In addition, the strain SXM-17 also has a growth-promoting effect on crops planted in natural planting environments and salinized environments. The strain SXM-17 provided by the present invention enriches the resource library of biocontrol strains against Gibberella zeae and the resources of green and efficient biological control microorganisms.

The invention relates to the technical field of films, in particular to a greenhouse film capable of preventing and treating aleyrodid diseases and insect pests and tomato physalospora piricola and a preparation method of the greenhouse film. According to the greenhouse film, new materials such as aluminum oxide coated aluminum nanoparticles, graphene nanosheets and double-brush modified silicon dioxide are introduced, so that deep inhibition of a UV-A high-sensitivity section and low-dosage retention of UV-B are realized, and the greenhouse film has excellent visible light transmittance and mechanical properties. The film can effectively inhibit aleyrodid colonization and physalospora piricola germination, improves the greenhouse planting effect, has high dispersity, low additive migration and good weather resistance, is suitable for prevention and control and green production of modern efficient agriculture, and has wide popularization prospects.

The invention discloses an isophorone derivative and application thereof, the isophorone derivative has excellent bacteriostatic action on rhizoctonia solani, botrytis cinerea, sclerotinia sclerotiorum, fusarium graminearum, valsa mali and physalospora piricola, and can be used for preventing and treating various plant fungal diseases.

The invention discloses a chalcone compound, and particularly discloses a structure of the compound. The invention also provides a preparation method and application thereof. A biological activity test result shows that the compound has relatively good inhibitory activity on six plant fungal diseases including lycium barbarum root rot bacteria, apple alternaria leaf spot bacteria, botrytis cinerea, celery leaf spot bacteria, valsa mali and potato black scurf bacteria, and the inhibitory activity of part of the compound on the bacteria is superior to that of azoxystrobin; the compound has a prospect of becoming a novel bactericide.

The present invention belongs to the field of pesticide chemistry, and relates to a pyrrolidine spirobisoxindole compound containing quinazolinone. The present invention discloses the molecular formula of the compound and also discloses its preparation method: substituting isatin, benzylamine and Schizocommunin are dissolved in a reaction solvent in proportion and reacted by the "one-pot method". After the reaction of Schizocommunin is detected to be complete by thin layer chromatography, the reaction solvent is removed under reduced pressure to obtain a crude product, which is obtained after recrystallization in a solvent. The method of the present invention does not require a catalyst, is simple and efficient. The raw materials are easily available and the atomic utilization rate is high; the operation is simple and convenient; the substrate range is wide and the functional group compatibility is good, and pyrrolidine spirobisoxindole compounds containing quinazolinone with rich structures can be obtained in high yields. The compounds disclosed by the present invention exhibit good activities against plant pathogenic fungi and can be used for controlling plant pathogenic fungi, especially Physalospora piricola Nose.

The invention provides a botryosphaeria dothidea effect protein BdNIS1 as well as a coding gene and application thereof. The amino acid sequence of the botryosphaeria dothidea effect protein BdNIS1 is shown as SEQ ID NO.1, and the nucleotide sequence of the coding gene of the botryosphaeria dothidea effect protein BdNIS1 is shown as SEQ ID NO.2 Research finds that after the BdNIS1 gene is knocked out, the growth of the botryosphaeria dothidea is not influenced, the stress resistance and the cell wall integrity of the botryosphaeria dothidea are not influenced, but the pathogenicity of the botryosphaeria dothidea to apple fruits and branches is remarkably reduced, so that the BdNIS1 is a key pathogenic gene in the botryosphaeria dothidea infection process. The BdNIS1 is transiently expressed in nicotiana benthamiana, PCD reaction caused by Bax in nicotiana benthamiana leaf cells can be inhibited, and infection of pathogenic bacteria to plants is promoted. The invention has important significance for analyzing the molecular mechanism related to the physalospora piricola and establishing the comprehensive prevention and control technical strategy of the physalospora piricola.

The invention discloses a phenylthiazole isoxazoline / isoxazole compound and application thereof as an agricultural bactericide, and relates to the technical field of organic synthesis of pesticides. The invention specifically provides a compound of phenyl thiazole isoxazoline or phenyl thiazole isoxazole type. According to the present invention, the two compounds having the general structural formula have good inhibition effects on crop infection fungi such as Botryodiplodia theobromae, Fusarium versicola, Magnaporthe oryzae, Diaporthe actinidia, Colletotrichum gloeosporioides, Physalospora piricola and the like under the condition of the low dose, can be used for preventing and treating diseases caused by the fungi, and further can be used for preventing and treating the crop infection, such that the crop infection prevention and treatment effects can be provided, and the crop infection prevention and treatment effects can be provided; and the selection diversity of the bactericide is increased.

The application discloses an arecoline ester derivative, a preparation method and application thereof, relates to the technical field of chemical pesticides, and has a brand-new molecular structure, and the bacteriostatic activity and insecticidal activity of the arecoline ester derivative are significantly enhanced compared with arecoline; the arecoline ester derivative has certain inhibiting effects on Puccinia recondita, Diplodia natalensis, Gibberella saubinetii, Pyricularia grisea, Phomopsis vexans, Physalospora piricola, Pestalotiopsis versiflorea, and Colletotrichum gloeosporioides; and the arecoline ester derivative also has certain insecticidal activity on Aedes aegypti larvae.

The invention provides a preparation method of a COS-dsRNA nano nucleic acid pesticide for preventing and treating apple ring rot, which comprises the following steps: carrying out in-vitro synthesis and purification on dsRNA of a key pathogenic effect gene BdNIS1 of targeted silence botryosphaeria dothidea to obtain dsRNA, and adding the dsRNA into a nano chitosan oligosaccharide aqueous solution to obtain the COS-dsRNA nano nucleic acid pesticide for preventing and treating botryosphaeria dothidea. The invention further provides application for preventing and treating apple ring spot. The exposed dsRNA and the nano chitosan oligosaccharide are compounded, the prepared COS-dsRNA nano nucleic acid pesticide has the effect of resisting the physalospora piricola, the expression quantity of pathogenic bacteria related target genes in the infection stage is obviously inhibited, and the infection capacity of the physalospora piricola is obviously reduced. The nano chitosan oligosaccharide is low in cost, easy to degrade in the environment, eco-friendly to the field and free of residues. Therefore, the RNAi nucleic acid pesticide has a wide application prospect in the field of prevention and control of physalospora piricola.