9 results about "Physalospora" patented technology

The invention discloses dsRNA of a targeted BdSpt7 gene, a biocontrol agent and application of the dsRNA. The sequence of the dsRNA of the targeted BdSpt7 gene is as shown in SEQ ID No.2. The invention further discloses a preparation method of the biocontrol agent of the targeted BdSpt7 gene. The active ingredient of the biocontrol agent is the dsRNA of the target BdSpt7 gene. The invention also provides an application of the botryosphaeria dothidea BdSpt7 gene in prevention and treatment of apple botryosphaeria dothidea, and a nucleotide sequence of the botryosphaeria dothidea BdSpt7 gene is shown as SEQ ID No.1. The invention also provides an African trichoderma harzianum engineering strain for expressing dsRNA, the African trichoderma harzianum engineering strain is integrated with a dsRNA expression box for interfering gene expression of a target gene BdSpt7, the dsRNA expression box is shown as SEQ ID No.1, and the African trichoderma harzianum is an African trichoderma harzianum with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 41709. The dsRNA targeting the BdSpt7 gene can significantly reduce the pathogenicity of the physalospora piricola through interference, and the African trichoderma harzianum engineering strain can colonize on the plant surface and continuously express the dsRNA so as to play a role in preventing and treating the physalospora piricola, and is green and environment-friendly.

The invention discloses application of a KASP molecular marker in identification of resistance to physalospora piricola of tea trees, and belongs to the technical field of plant molecular biology. The invention aims to develop the KASP molecular marker closely linked with the resistance of the tea tree physalospora piricola and accelerate the process of assisted breeding of the molecular marker with the resistance of the tea tree physalospora piricola. The KASP marker for identifying the resistance to the physalospora piricola of the tea tree is Cs-KASP, and the marker is positioned to an SNP related to identification of the resistance to the physalospora piricola of the tea tree through generic genome sequence alignment. A group of KASP molecular markers closely linked with the resistance of the tea tree physalospora piricola are developed according to the SNP site. The marker can be used for genotype detection of different resistant materials of the tea tree physalospora piricola, can be used for successfully distinguishing a disease-resistant material from a disease-susceptible material, and is combined with a high-throughput genotyping detection system to quickly identify physalospora piricola-resistant germplasm resources, so that the breeding efficiency is improved.

The invention relates to the technical field of gene engineering, in particular to application of a CsMAPK3 gene in resisting physalospora piricola of tea trees, the base sequence of the CsMAPK3 gene is shown as SEQ ID NO.3, and the resistance of the tea trees to physalospora piricola is increased through overexpression of the CsMAPK3 gene. The regulation and control mechanism of the CsMAPK3 gene disclosed in the invention on the resistance of the tea tree physalospora piricola provides a basis for the subsequent application of the CsMAPK3 gene to the resistance of the tea tree physalospora piricola.

The invention provides a strain of bacillus siamensis Bsiamensis and application thereof, the strain is classified and named as bacillus siamensis Bsiamensis, the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO: 35785, and the preservation date is September 1, 2025. The bacterial suspension, the fermentation liquor and the ethyl acetate extract of the fermentation liquor of the bacterial strain have good bacteriostatic activity on fusarium graminearum, fusarium oxysporum, fusarium laminatum, alternaria longibrachiata, colletotrichum gloeosporioides and colletotrichum frutescens.

The invention relates to the technical field of films, in particular to a greenhouse film capable of preventing and treating aleyrodid diseases and insect pests and tomato physalospora piricola and a preparation method of the greenhouse film. According to the greenhouse film, new materials such as aluminum oxide coated aluminum nanoparticles, graphene nanosheets and double-brush modified silicon dioxide are introduced, so that deep inhibition of a UV-A high-sensitivity section and low-dosage retention of UV-B are realized, and the greenhouse film has excellent visible light transmittance and mechanical properties. The film can effectively inhibit aleyrodid colonization and physalospora piricola germination, improves the greenhouse planting effect, has high dispersity, low additive migration and good weather resistance, is suitable for prevention and control and green production of modern efficient agriculture, and has wide popularization prospects.

The invention discloses an isophorone derivative and application thereof, the isophorone derivative has excellent bacteriostatic action on rhizoctonia solani, botrytis cinerea, sclerotinia sclerotiorum, fusarium graminearum, valsa mali and physalospora piricola, and can be used for preventing and treating various plant fungal diseases.

The invention discloses a chalcone compound, and particularly discloses a structure of the compound. The invention also provides a preparation method and application thereof. A biological activity test result shows that the compound has relatively good inhibitory activity on six plant fungal diseases including lycium barbarum root rot bacteria, apple alternaria leaf spot bacteria, botrytis cinerea, celery leaf spot bacteria, valsa mali and potato black scurf bacteria, and the inhibitory activity of part of the compound on the bacteria is superior to that of azoxystrobin; the compound has a prospect of becoming a novel bactericide.

The application discloses an arecoline ester derivative, a preparation method and application thereof, relates to the technical field of chemical pesticides, and has a brand-new molecular structure, and the bacteriostatic activity and insecticidal activity of the arecoline ester derivative are significantly enhanced compared with arecoline; the arecoline ester derivative has certain inhibiting effects on Puccinia recondita, Diplodia natalensis, Gibberella saubinetii, Pyricularia grisea, Phomopsis vexans, Physalospora piricola, Pestalotiopsis versiflorea, and Colletotrichum gloeosporioides; and the arecoline ester derivative also has certain insecticidal activity on Aedes aegypti larvae.

The invention provides a preparation method of a COS-dsRNA nano nucleic acid pesticide for preventing and treating apple ring rot, which comprises the following steps: carrying out in-vitro synthesis and purification on dsRNA of a key pathogenic effect gene BdNIS1 of targeted silence botryosphaeria dothidea to obtain dsRNA, and adding the dsRNA into a nano chitosan oligosaccharide aqueous solution to obtain the COS-dsRNA nano nucleic acid pesticide for preventing and treating botryosphaeria dothidea. The invention further provides application for preventing and treating apple ring spot. The exposed dsRNA and the nano chitosan oligosaccharide are compounded, the prepared COS-dsRNA nano nucleic acid pesticide has the effect of resisting the physalospora piricola, the expression quantity of pathogenic bacteria related target genes in the infection stage is obviously inhibited, and the infection capacity of the physalospora piricola is obviously reduced. The nano chitosan oligosaccharide is low in cost, easy to degrade in the environment, eco-friendly to the field and free of residues. Therefore, the RNAi nucleic acid pesticide has a wide application prospect in the field of prevention and control of physalospora piricola.