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48 results about "Bipolaris maydis" patented technology

Southern corn leaf blight (SCLB) is a fungal disease of maize caused by the plant pathogen Bipolaris maydis (also known as Cochliobolus heterostrophus in its teleomorph state). The fungus is an Ascomycete and can use conidia or ascospores to infect.

Trifluoromethyl-containing pyrimidinamine compound, preparation method thereof, and application of trifluoromethyl-containing pyrimidinamine compound used as bacteriacide

InactiveCN102617482AHigh bactericidal activityBiocideOrganic chemistryDiseaseExserohilum species
The invention relates to a 4-substituted phenyl-6-trifluoromethyl-2-amino pyrimidinamine compound which has a novel chemical structure and a general formula III, a synthesis method for the compound and the application of the compound used as bacteriacide. The method comprises the following steps of: reacting substituted phenyl methyl ketone used as an initial raw material and trifluoroacetic acid ethyl ester under catalysis of sodium alcoholate to generate 4,4,4-trifluoro-1-substituded phenylbutane-1,3-diketone, and reacting 4,4,4-trifluoro-1-substituded phenylbutane-1,3-diketone and guanidinium salt under an alkaline condition to generate a III series compound with the general formula III. The compound with the general formula III can inhibit growth of pathogenic bacteria such as rice sheath blight, rice bakanae disease, exserohilum turcicum, bipolaris maydis, curvulavia lunata, corn kernel rot, corn top rot pathogenic bacteria, fusarium graminearum, cotton anthracnose, bean fusarium, rape sclerotinia rot, botrytis cinerea, cucumber fusarium wilt pathogenic bacteria, cucumber brown rot pathogenic bacteria, cucumber scab pathogenic bacteria, hot pepper root rot pathogenic bacteria, kidney bean anthracnose bacteria, and pythium aphanidermatum. In the formula III, R is methyl, ethyl, methoxy group, fluorine, chlorine, bromine, trifluoromethyl and other groups, and the number of R is 1 to 3.
Owner:SHENYANG AGRI UNIV

Corn southern leaf blight field natural inducement technology and application of technology

InactiveCN106416654AEasy to getFully reflect the ability to resist diseases and expand vertically upwardsPlant cultivationCultivating equipmentsDiseased plantPlastic mulch
The invention relates to the technical field of crop disease research, in particular to a corn southern leaf blight field natural inducement technology and application of the technology. The technology basically includes the steps that plastic film mulching is conducted; individual core seedlings which all have 3-4 leaves and 1 core are planted in two rows, wherein the line spacing is 0.4-0.5 m; in the stage when each corn plant in the field has 7-8 leaves, fresh corn southern leaf blight diseased plants artificially inoculated in a greenhouse serve as a field inducement bacterium source and horizontally placed on moist surfaces between the corn plants, and field normal irrigation is used for keeping soil moist. The infected corn southern leaf blight diseased plants which are artificially inoculated serve as the inducement bacterium source, operation is easy, and the inducement bacterium source is easy to obtain. The structure of the inducement bacterium source is similar to that of a wild bipolaris maydis population, and the pathogenic ability is reliable. Good and stable pathogen conditions can be provided. The technology is applicable to corn southern leaf blight resistance screening of corn germplasm and also applicable to screening of disease prevention insecticides and technical research.
Owner:INST OF PLANT PROTECTION FAAS

Efficient disease-resistant authenticated inoculum for corn leaf blight and preparation method thereof

The invention provides an efficient disease-resistant authenticated inoculum for corn leaf blight and a preparation method thereof. The inoculum comprises the following raw materials in proportion byweight: 100 g of sorghum, 0.20 to 0.25 g of magnesium sulfate, 0.20 to 0.25 g of sodium phosphate and 15 to 30 g of corn leaves. The preparation method comprises the following steps: (1) screening ofcorn leaf blight virulent bacterial strains; (2) storage of corn leaf blight virulent bacterial strains; (3) preparation of secondary cultures; (4) preparation of the inoculum; and (5) cultivation ofthe inoculum. The inoculum has the advantages that the obtained bacterial strains for authenticating corn leaf blight disease resistance are bacterial strains with high pathogenicity, high purity andhigh activity, and guarantees are provided for authenticating corn disease resistance inoculation; the work efficiency is greatly improved compared with a plate medium; the infection of gnats and infectious microbes is avoided, so that the high-quality inoculum finished product is obtained; the cultivation of the inoculum and a cultivation condition thereof are beneficial for generation of corn leaf blight germ conidium, so that the inoculum is high in spore formation speed, large in spore formation quantity and long in spore formation period.
Owner:GUANGXI AGRI VOCATIONAL COLLEGE

Polyketide synthase PreuA and application thereof in preparation of red powder moss acid

The invention relates to a polyketide synthase and an application thereof in preparation of red powder moss acid, and belongs to the technical field of microbial chemistry. According to the invention, the polyketide synthase PreuA related to synthesis of the rubrorubicacid is cloned from the phorbia fungus for the first time, and a yeast mutant strain capable of efficiently producing the rubrorubicacid is constructed based on a saccharomyces cerevisiae heterologous expression technology; and the prepared red powder moss acid is taken as an object, and the inhibitory activity of the red powder moss acid on seven kinds of crop pathogenic fungi is researched. Research finds that the red powder moss acid has different degrees of inhibition effects on four crop pathogenic fungi including apple ring spot bacteria, corn bipolaris maydis, potato verticillium wilt bacteria and sclerotium rolfsii, and has a very strong antagonistic effect (MIC, 25 [mu]g / mL) on the apple ring spot bacteria. The invention greatly enriches the production source of the red powder moss acid, and has important scientific value and application prospect for expanding the derivatization approach of the red powder moss acid and researching and developing novel biopesticides.
Owner:SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES

Rapid detection method of pathogens of corn southern leaf blight

InactiveCN105543382AMonitor for latent infestationTake early and effective preventive measuresMicrobiological testing/measurementForward primerA-DNA
The invention belongs to the technical field of gene engineering, relates to a method for rapidly detecting pathogens by a specific primer, and in particular relates to the specific primer for rapidly detecting corn southern leaf blight and a detection method of the corn southern leaf blight. A rapid detection method of pathogens of the corn southern leaf blight comprises the following steps: (1) collecting fungal hyphae; (2) extracting fungal DNAs; (3) carrying out PCR (Polymerase Chain Reaction) amplification, wherein a PCR system is as follows: 1 microliter of a forward primer X-EF, 1 microliter of a reverse primer X-ER, 1 microliter of a DNA template, 12.5 microliters of Taq PCR Master Mix, and the balance of ddH2O which is added until the volume is 25 microliters; a PCR procedure: pre-denaturing for 3 minutes at 94 DEG C; denaturing for 30 seconds at 94 DEG C, annealing for 30 seconds at 52.9 DEG C, and extending for 30 seconds at 72 DEG C, circulating for 32 times; extending for 10 minutes at 72 DEG C and preserving at 4 DEG C. In order to provide evidences for rapidly and accurately monitoring whether corn leaves are latently infected by bipolaris maydis or not, prevention and control measures can be easily and effectively adopted as soon as possible.
Owner:HENAN AGRICULTURAL UNIVERSITY

Trifluoromethylpyrimidine-containing ammonia compound, preparation method and use as bactericide

InactiveCN102617482BHigh bactericidal activityBiocideOrganic chemistryExserohilum speciesKetone
The invention relates to a 4-substituted phenyl-6-trifluoromethyl-2-amino pyrimidinamine compound which has a novel chemical structure and a general formula III, a synthesis method for the compound and the application of the compound used as bacteriacide. The method comprises the following steps of: reacting substituted phenyl methyl ketone used as an initial raw material and trifluoroacetic acid ethyl ester under catalysis of sodium alcoholate to generate 4,4,4-trifluoro-1-substituded phenylbutane-1,3-diketone, and reacting 4,4,4-trifluoro-1-substituded phenylbutane-1,3-diketone and guanidinium salt under an alkaline condition to generate a III series compound with the general formula III. The compound with the general formula III can inhibit growth of pathogenic bacteria such as rice sheath blight, rice bakanae disease, exserohilum turcicum, bipolaris maydis, curvulavia lunata, corn kernel rot, corn top rot pathogenic bacteria, fusarium graminearum, cotton anthracnose, bean fusarium, rape sclerotinia rot, botrytis cinerea, cucumber fusarium wilt pathogenic bacteria, cucumber brown rot pathogenic bacteria, cucumber scab pathogenic bacteria, hot pepper root rot pathogenic bacteria, kidney bean anthracnose bacteria, and pythium aphanidermatum. In the formula III, R is methyl, ethyl, methoxy group, fluorine, chlorine, bromine, trifluoromethyl and other groups, and the number of R is 1 to 3.
Owner:SHENYANG AGRI UNIV
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