20 results about "Bipolaris maydis" patented technology

The invention belongs to the technical field of agricultural science, and particularly provides application of oligosaccharide in prevention and treatment of corn southern leaf blight. The oligosaccharide has an inhibiting effect on the bipolaris maydis and can be used as an inhibitor of the bipolaris maydis. Meanwhile, the oligosaccharide can be used as a plant immune resistance inducer for preventing and treating the corn southern leaf blight, and the occurrence and development of the corn southern leaf blight are prevented and controlled by inducing basic resistance and enhancing the plant immunity. The oligosaccharide application method is simple and convenient to operate, green and environment-friendly, green prevention and control are facilitated, and grain loss caused by diseases is reduced more economically and more environmentally.

The invention belongs to the field of microorganisms, and particularly relates to a banana endophyte compound fungicide as well as a preparation method and application thereof. According to the invention, a novel endophytic bacillus velezensis JDB15 strain and an endophytic trichoderma harzianum JDL4 strain are separated from a banana plant. The trichoderma harzianum JDL4 can inhibit botrytis cinerea, fusarium graminearum, rhizoctonia solani, peach anthracnose pathogen, peach brown rot pathogen and alternaria alternata, and has a good broad-spectrum antibacterial effect. Meanwhile, after the filtrate of the fermentation liquor of the trichoderma harzianum JDL4 and the bacillus velezensis JDB15 are compounded, the antibacterial effect is remarkably improved, and the control effect on banana fusarium wilt, pyricularia oryzae, tomato fusarium wilt and corn bipolaris maydis is also remarkably improved. The compound bacterial agent has wide biocontrol bacterial activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other plant diseases.

The invention belongs to the field of microorganisms, and particularly relates to bacillus velezensis with high stress resistance, bacterial powder, a biocontrol microbial inoculum and preparation and application thereof. The bacillus velezensis is bacillus velezensis Shannan.BV80-12 and is preserved in the China Center for Type Culture Collection, and the preservation number of the bacillus velezensis is CCTCC (China Center for Type Culture Collection) NO: M 2024386. The bacillus velezensis Shannan.BV80-12 provided by the invention has relatively strong tolerance to drought, and has the capability of producing indoleacetic acid (IAA). Meanwhile, the bacterial strain also has a broad-spectrum antibacterial ability, and has a relatively strong inhibition effect on fusarium graminearum, alternaria alternata, magnaporthe oryzae, bipolaris maydis, exserohilum turcicum, sclerotinia sclerotiorum, physalospora piricola, alternaria solani and citrus brown rot pathogen.

The invention relates to bacillus paralicheniformis CBJ-7, and the preservation number of the bacillus paralicheniformis CBJ-7 is CGMCC (China General Microbiological Culture Collection Center) No.31960. The bacillus paralicheniformis CBJ-7 has the advantages that the preservation number is CGMCC No.31960; the bacillus paralicheniformis disclosed by the invention has the capacity of producing cellulase and amylase, and has an obvious antagonistic effect on fusarium oxysporum of cotton and bipolaris maydis. When applied to anaerobic fermentation of agricultural organic wastes, the biogas yield can be increased by more than 15%; when being used for composting the livestock and poultry manure, the organic fertilizer can effectively improve the fermentation temperature and shorten the decomposition time, and has important significance on promoting the recycling and energy utilization of organic wastes.

The present application relates to the application of allantoin in the prevention and treatment of fungal diseases of grain crops. The present application finds that allantoin can inhibit the growth of pathogenic fungi of grain crops, especially inhibit the infection of pathogenic fungi of grain crops to host cells or inhibit the expansion of pathogenic fungi of grain crops in host cells, thus indicating that allantoin can be used as a pesticide to prevent and treat pathogenic fungi of grain crops, especially Magnaporthe oryzae, Cochliobolus miyabeanus and Ustilaginoidea virens.

This invention belongs to the field of biological control technology for plant diseases and pests, and provides a strain of Chaetomium globosum ( Chaetomium globosum EF-14 and its applications. This strain is deposited at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 40618. The *Chaetomium globosum* EF-14 provided by this invention is an endophytic fungus of maize that can simultaneously inhibit *Fusarium graminearum* (…). Fusarium gramineae ) and Corn spores ( Bipolaris maydis It can effectively reduce corn stalk rot and corn leaf spot caused by fall armyworm ( ) and can also effectively reduce the incidence of fall armyworm ( ). Spodoptera frugiperda This invention provides a highly efficient and safe microbial resource for the green control of major corn diseases and pests, and broadens the application scope of Chaetomium globosum in agricultural biocontrol, achieving synergistic control of diseases and pests through "one fungus targeting multiple targets".

The invention relates to the technical field of culture devices, and discloses a bipolaris maydis culture and observation device which comprises a culture box, a box door arranged on the culture box, an observation window arranged on the box door and a display panel arranged on the side wall of the culture box. A groove is formed in the middle of the placement frame, and a mounting seat is arranged in the culture cavity; through cooperative arrangement of the driving system, the mounting base and the macro camera in the culture cavity, the function of observing the growth condition of the bipolaris maydis in the culture dish without opening the box door is achieved, and the driving system can drive the mounting base to accurately move in the X direction, the Y direction and the Z direction; the microspur camera can move to the position over any target culture dish, shot images are displayed in real time through the display panel, and the problem that in a traditional observation mode, due to the fact that a box door is opened, external unfiltered and disinfected air enters a culture cavity is effectively solved.

The application belongs to the field of antibacterial activity research, and discloses a preparation method of guaiane sesquiterpenes and application thereof in resisting plant pathogenic fungi. Firstly, leaf blades of Artemisia stolonifera are dried, extracted, concentrated, and then a crude fraction is obtained through extraction, and then the crude fraction is further purified through normal silica gel, dextran gel and reversed-phase macroporous resin column chromatography, and finally three guaiane sesquiterpenes are obtained through liquid phase preparation. The compounds can effectively inhibit the growth of Sclerotinia sclerotiorum, Cochliobolus sativus and Verticillium dahliae, and the minimum inhibitory concentration (MIC) of moxartenolide is 6.25, 12.5 and 12.5 μg / mL respectively, the minimum inhibitory concentration (MIC) of artemdubolide C is 12.5, 12.5 and 12.5 μg / mL respectively, and the minimum inhibitory concentration (MIC) of artemvulactone F is 12.5, 12.5 and 12.5 μg / mL respectively, and the compounds have great application potential in resisting plant pathogenic fungi.

The invention discloses an antagonistic indigenous bacterium UVK8 for efficiently preventing and treating rice false smut and application of the antagonistic indigenous bacterium UVK8. The antagonistic indigenous bacterium UVK8 is classified and named as bacillus amyloliquefaciens, and is preserved in the China General Microbiological Culture Collection Center on June 11, 2025, the preservation number is CGMCC NO.34861, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The antagonistic indigenous bacterium UVK8 shows excellent antagonistic action and bacteriostatic activity on rice ustilaginoidea virens, watermelon fusarium oxysporum, peach monilinia fusarium, banana fusarium oxysporum, exserohilum turcicum, bipolaris maydis and orchid fusarium oxysporum, and has relatively high biofilm content and certain rice straw degradation capability. Field test results show that the antagonistic indigenous bacterium UVK8 has a good prevention and treatment effect on rice false smut, the disease clump prevention effect reaches 86.28%, and the clump disease grain number prevention effect reaches 58.03%. Therefore, the antagonistic indigenous bacterium UVK8 has an important biocontrol application value.

The present invention relates to a Bacillus paralicheniformis ( Bacillus paralicheniform ) CBJ-7, with a deposit number of CGMCC No. 31960. The Bacillus paralicheniformis strain of the present invention is capable of producing cellulase and amylase, and has a significant antagonistic effect against cotton wilt pathogen and corn leaf spot pathogen. When applied to anaerobic fermentation of agricultural organic waste, it can increase biogas production by more than 15%. When used in livestock and poultry manure composting, it can effectively increase fermentation temperature and shorten composting time, which is of great significance for promoting the resource and energy utilization of organic waste.

The present invention belongs to the field of microorganisms, and specifically relates to a banana endophyte compound agent, a preparation method, and an application thereof. The present invention isolates a novel endophytic Bacillus Velez JDB15 and an endophytic Trichoderma harzianum JDL4 from banana plants. The Trichoderma harzianum JDL4 can inhibit Botrytis cinerea, Fusarium graminearum, Rhizoctonia solani, Colletotrichum syringae prunellae, Peach anthracnose, Peach brown rot, and Alternaria alternata, and has a good broad-spectrum antibacterial effect. At the same time, the filtrate of the fermentation broth of Trichoderma harzianum JDL4 is compounded with Bacillus Velez JDB15 to significantly enhance the antibacterial effect, and the control effect against banana wilt pathogen, rice blast pathogen, tomato wilt, and corn leaf spot pathogen is also significantly enhanced. The compound agent has a wide range of biocontrol activities and can be further developed in the future to provide a sustainable and green solution for the control of banana wilt and other plant diseases.

The invention belongs to the technical field of biological detection, and particularly relates to a sequence combination for rapidly detecting Paphanerospora maydis based on RPA-CRISPR / Cas12a and application of the sequence combination. The invention relates to a sequence combination for rapidly detecting Philospora maydis based on RPA-CRISPR / Cas12a, which is characterized in that the sequence combination comprises RPA (recombinase polymerase amplification) primers Bm-F3 / Bm-R3 and Bm-crRNA (complementary ribosomal ribonucleic acid) sequences. The specific RPA primer is designed for the bipolaris maydis which is high in separation frequency, wide in distribution range and serious in harm, and field visual rapid detection of the bipolaris maydis can be achieved by combining recombinase polymerase amplification with a CRISPR / Cas12a detection technology. The technology is easy and convenient to operate, reaction can be carried out under the constant-temperature condition, expensive instruments are not needed, and detection time is short. Aerosol pollution caused by secondary cover opening can also be avoided by using a one-tube method for detection, meanwhile, the specificity and sensitivity of RPA detection can be improved by using a CRISPR / Cas12a system, and an efficient and reliable detection scheme can be provided for field monitoring of the Philospora maydis.

The invention belongs to the technical field of molecular biological detection, and particularly relates to a primer pair, a detection reagent and an RPA-LFD kit for visually, ultrasensitively and rapidly detecting puccinia zeae and application. The sequences of an upstream primer and a downstream primer of the RPA primer pair are respectively shown as SEQ ID NO: 1 and SEQ ID NO: 2. The RPA primer pair can be used for visually detecting the puccinia zeae through RPA amplification or RPA-LFD by naked eyes, the sensitivity is ultrahigh (0.01 pg / mu L), and the specificity is strong; and no specific band exists on puccinia polystachys, bipolaris maydis, corn ear rot pathogen and curvularia maydis leaf spot pathogen.

The invention discloses specific primers and a kit for identifying microspecies O and microspecies T of bipolaris maydis and application of the specific primers and the kit. The specific primers comprise an upstream primer and a downstream primer for detecting the O race and an upstream primer and a downstream primer for detecting the T race, the nucleotide sequence of the O race upstream primer is shown as SEQ ID NO.1, and the nucleotide sequence of the downstream primer is shown as SEQ ID NO.2; the nucleotide sequence of the upstream primer of the T races is shown as SEQ ID NO.3, and the nucleotide sequence of the downstream primer of the T races is shown as SEQ ID NO.4. On the basis of the primers, a PCR (Polymerase Chain Reaction) detection system for the O races and the T races of the P. maydis is established. The specific primer provided by the invention has the advantages of strong specificity, high sensitivity, high accuracy, rapidness, simplicity and convenience in operation and the like when being used for detecting the O microspecies and the T microspecies, and can be used for effectively identifying the O microspecies and the T microspecies of the P. maydis.

The invention discloses multifunctional cold-resistant bacillus subtilis and application thereof, and belongs to the technical field of microorganisms. According to the invention, a strain of the Peribacillusfrigitorirans YM763 is obtained through separation and screening, and the strain can be used for preventing and treating corn diseases caused by Curcularia lunata, Exserohilum tubers and Bipolar maydis at the same time and degrading a chemical insecticide chlorantraniliprole, and has no cross resistance with existing bactericides and insecticides. The invention further provides a preparation method of the strain of the Peribacillusfrigitorirans YM763, and the strain of the invention has the advantages that the strain of the Peribacillusfrigitorirans YM763 can be used for preventing and treating corn diseases caused by Curcularia lunata, Exserohilum tubers and Bipolar maydis and degrading the chemical insecticide chlorantraniliprole; compared with the existing chemical synthesis pesticide for prevention and treatment, the method has the advantages of high efficiency, low toxicity, no environmental pollution and low possibility of generating drug resistance because of being from the natural environment.

The application provides a primer probe combination, a kit and a detection method for LAMP-LFD detection of corn small spot fungus, and belongs to the technical field of biology.The primer probe combination for LAMP-LFD detection of corn small spot fungus comprises an external upstream primer F3, an external downstream primer B3, an internal upstream primer FIP, an internal downstream primer BIP and a probe Bm-HP.The detection primer probe combination is used to take the genomic DNA of a sample to be detected as a template, and after LAMP constant-temperature amplification, LFD test paper is used for detection.If the quality control band and the detection band both show color, it indicates that the corn small spot fungus detection result of the sample is positive.The primer probe combination has good specificity, high accuracy, high sensitivity, and the detection limit is 1fg. ‑1 μL, is simple, fast and easy to operate, and has important significance for early warning and prevention and control of corn small spot, and prevention and control of the spread and spread of diseases.

The invention relates to application of allurea in prevention and treatment of fungal diseases of food crops. The invention finds that the allurea can inhibit the growth of the pathogenic bacteria of the food crops, especially inhibit the pathogenic bacteria of the food crops from infecting host cells or inhibiting the pathogenic bacteria of the food crops from expanding in the host cells, so that the allurea can be used as a pesticide to prevent and inhibit the pathogenic bacteria of the food crops, especially pyricularia grisea, bipolaris maydis and ustilaginoidea virens.

The invention provides a primer probe combination, a kit and a detection method for corn bipolaris maydis LAMP-LFD (loop-mediated isothermal amplification-lateral flow dipstick) detection, and belongs to the technical field of biology. The primer and probe combination for LAMP-LFD detection of the bipolaris maydis comprises an external upstream primer F3, an external downstream primer B3, an internal upstream primer FIP, an internal downstream primer BIP and a probe Bm-HP. According to the detection primer probe combination, genome DNA of a to-be-detected sample is used as a template, LAMP isothermal amplification is performed, then the LFD test strip is used for detection, and if a quality control band and a detection band both develop colors, it is indicated that the detection result of the corn bipolaris maydis of the sample is positive. The primer probe combination is good in specificity, high in accuracy and high in sensitivity, the detection limit is 1 fg.mu L <-1 >, operation is easy, convenient and rapid, and the primer probe combination has important significance on early warning, prevention and control of corn southern leaf blight and prevention and control of disease diffusion and spreading.

The invention belongs to the technical field of microorganisms, and particularly relates to a rubber tree red root disease biocontrol bacterium QHL1 and application thereof. The biocontrol bacterium QHL1 for the red root disease of the rubber tree, disclosed by the invention, has broad-spectrum bacteriostatic activity, and has a good antagonistic effect on red root pathogen of the rubber tree, rhizoctonia solani, fusarium moniliforme, eggplant fusarium wilt pathogen, alternaria solani, fusarium graminearum, wheat root rot pathogen, phytophthora parasitica var nicotianae, alternaria alternata, bipolaris maydis and the like; the biocontrol bacterium is a potential biological agent for preventing and treating hevea brasiliensis red root diseases, rice bakanae diseases, eggplant fusarium wilt and the like, and also has good capabilities of producing protease, cellulase, beta-glucanase, amylase, siderophore and the like. The biocontrol bacterium QHL1 for the red root disease of the rubber tree has a wide development space and a good development and application prospect, and provides a candidate resource for prevention and treatment of the red root disease of the rubber tree and other plant diseases.

A special fertilizer for corn suitable for saline-alkali soil, a preparation method therefor, and a use thereof, belonging to the technical field of fertilizers. The special fertilizer comprises a modifier and a compound microbial agent. The modifier comprises veratramine, zeolite powder, biochar, and humic acid, and the dosage ratio of the veratramine, the zeolite powder, the biochar, and the humic acid is (3-6):(3-5):(1-2):(5-8). The compound microbial agent comprises Cryptococcus and Trichothecium roseum, the preservation number of the Cryptococcus is GDMCC 2.205, and the preservation number of the Trichothecium roseum is GIM 3.500. The introduction of the modifier and the compound microbial agent into a traditional base fertilizer enables said fertilizer to effectively improve the quality of corn, and also prevent and control the infection of corn by Bipolaris maydis.