Rapid detection method of pathogens of corn southern leaf blight

A technology of corn spot disease and detection method, which is applied in the field of specific primers for rapid detection of corn spot spot and its detection field, which can solve the problems of difficult pathogenic bacteria, long time-consuming, strong experience, etc.
CN105543382AInactive Publication Date: 2016-05-04HENAN AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HENAN AGRICULTURAL UNIVERSITY
Publication Date
2016-05-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the technical field of gene engineering, relates to a method for rapidly detecting pathogens by a specific primer, and in particular relates to the specific primer for rapidly detecting corn southern leaf blight and a detection method of the corn southern leaf blight. A rapid detection method of pathogens of the corn southern leaf blight comprises the following steps: (1) collecting fungal hyphae; (2) extracting fungal DNAs; (3) carrying out PCR (Polymerase Chain Reaction) amplification, wherein a PCR system is as follows: 1 microliter of a forward primer X-EF, 1 microliter of a reverse primer X-ER, 1 microliter of a DNA template, 12.5 microliters of Taq PCR Master Mix, and the balance of ddH2O which is added until the volume is 25 microliters; a PCR procedure: pre-denaturing for 3 minutes at 94 DEG C; denaturing for 30 seconds at 94 DEG C, annealing for 30 seconds at 52.9 DEG C, and extending for 30 seconds at 72 DEG C, circulating for 32 times; extending for 10 minutes at 72 DEG C and preserving at 4 DEG C. In order to provide evidences for rapidly and accurately monitoring whether corn leaves are latently infected by bipolaris maydis or not, prevention and control measures can be easily and effectively adopted as soon as possible.
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Description

technical field

[0001] The invention belongs to the technical field of genetic engineering, and relates to a method for rapidly detecting pathogens with specific primers, in particular to a specific primer for rapidly detecting maize leaf spot and a detection method thereof. Background technique

[0002] Corn small spot bacterium is half-knowledge fungus, Helminthosporium genus, Helminthospora maize, formal name used at school: Bipolarismaydis (NisikadoeTMiyake) Shoem, synonym: HelminthosporiummaydisNisikadoeTMiyake, Drechsleramaydis (NisikadoetMiyake) Subram&Jain. The sex is Cochliobolus Heterostrophus (Drechsler) Drechsler, and its synonym is: Ophiobolus Heterostrophus Drechsler. The fungal ascus is black, nearly spherical, 357-642×276-443 (μm), the apex of the ascus is blunt, and the base has a short stalk, the size is 124-183×23-29 (μm). The number of ascospores in each ascus varies, generally about three, and the ascospores are long linear, about 7.3×6.3-8.8 (μm) in si...

Claims

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