22results about How to "Rapid identification" patented technology

This invention relates to a method for establishing and detecting the fingerprint spectrum of the traditional Chinese medicine composition Taohongyin. The raw materials of the traditional Chinese medicine composition include: chicken gizzard lining, papaya, hawthorn, chrysanthemum, tangerine peel, peach kernel, and wolfberry. The fingerprint spectrum establishment method includes the preparation of a reference solution and a test solution; detection of the reference solution and the test solution using high-performance liquid chromatography (HPLC); the mobile phase used in the HPLC is acetonitrile and phosphoric acid aqueous solution, and the elution method is gradient elution.

This invention provides the application of phosphatidylinositol (16:0 / 18:3(9 cis, 12 cis, 15 cis)) in the identification of Hainan camellia, relating to the field of food testing technology. The phosphatidylinositol (16:0 / 18:3(9 cis, 12 cis, 15 cis)) can be used as a characteristic marker for the identification of Hainan camellia. This invention is the first to discover that the kernels of Hainan camellia contain high levels of phosphatidylinositol (16:0 / 18:3(9 cis, 12 cis, 15 cis)), while phosphatidylinositol (16:0 / 18:3(9 cis, 12 cis, 15 cis)) is not found or present in extremely low amounts in the kernels of other camellia species besides Hainan camellia. Phosphatidylinositol (16:0 / 18:3(9 cis, 12 cis, 15 cis)) can be used to identify and distinguish Hainan camellia from other camellia species. Furthermore, this identification method is simple to operate and achieves rapid, objective, and accurate identification of Hainan camellia.

The application discloses a primer pair, a kit and a method for detecting the expression amount of a liver function gene HNF1A. The primer pair comprises a forward primer as shown in SEQ ID NO:1 and a reverse primer as shown in SEQ ID NO:2. The primer pair of the application can realize monitoring of the relative expression amount of the HNF1A gene in a liver function cell product, and can realize rapid identification of liver function cells and relative expression of the HNF1A function gene of the cells, and the method is simple and convenient to operate.

The application discloses a SSR molecular marker primer related to Chinese chives dormancy and a detection method and application thereof. In view of the problems that traditional Chinese chives dormancy trait identification relies on field morphological observation for 4 months, is inefficient and lacks special molecular markers, 7 specific SSR primers (SSR005 / 170 / 249 / 325 / 423 / 478 / 610) are developed based on cross-species genome resequencing (taking Chinese onion as a reference genome, and the alignment rate is 18.76%-18.89%) for the first time. Through the establishment of a matching detection system: CTAB method for rapid DNA extraction, optimization of the PCR system (20 muL contains 30 ng of template DNA, 0.4 U of Taq enzyme, 0.2 muM of primer, 0.2 mM of Mg 2+ 2.0 mM of dNTPs, 57 DEG C annealing), 8% polyacrylamide gel electrophoresis combined with silver staining development, the dormancy trait can be accurately identified within 7 days. For example, the SSR423 primer has no band at 177 bp, and the non-dormant / dormant germplasm cannot be distinguished. The detection results of 52 germplasms (such as dormancy germplasm 21-1 and non-dormancy germplasm 22-7) are consistent with the field phenotypes at a rate of 100%.

The utility model discloses a kind of intelligent key cabinet with display screen, comprising: cabinet, intelligent control panel and key management kit, key management kit includes: key management cavity, door plate, electric lock, RFID sensing module and electronic display screen, and the key stored in key management kit is attached with RFID chip.On the key of key management kit, RFID chip is detected and identified by RFID sensing module, and detection information is fed back to intelligent control panel, and the vehicle code corresponding to key is displayed on the corresponding electronic display screen, so that the vehicle information corresponding to the key stored in key management kit is conveniently and quickly identified. Before user needs to take the vehicle corresponding key, whether the required vehicle key has been lent out can be identified by the information displayed on electronic display screen, so as to improve the efficiency of key lending and returning, and facilitate the storage and lending management of vehicle key.

The application discloses a detection method of Baizhu San standard decoction fingerprint combined with liquid chromatography-mass spectrometry, the fingerprint and application thereof, and belongs to the field of traditional Chinese medicine quality standard research and application. Test sample solution and single control sample solution are injected into a high performance liquid chromatograph to obtain respective chromatograms; mass spectrometry analysis is conducted on the test sample solution to obtain mass spectrometry results; common peaks existing in the chromatogram of the test sample solution are taken as common peaks, chemical components in the Baizhu San standard decoction are identified in combination with the chromatogram of the single control sample solution and the mass spectrometry results of the test sample solution, and the Baizhu San standard decoction fingerprint is obtained. The fingerprint is constructed through liquid chromatography-mass spectrometry, 10 intersecting components are identified in combination with the mass spectrometry information of the control sample, so that the quality control of the Baizhu San standard decoction is realized, the method is simple, easy to operate, high in precision, good in stability and reproducibility, and can effectively solve the technical problem that the quality of the Baizhu San cannot be effectively evaluated.

The application belongs to the technical field of soybean molecular genetics and breeding, and discloses a KASP marker for detecting a SNP site related to a soybean petiole angle and application thereof. The SNP site is located at position 4589285 on chromosome 10 of a soybean reference genome Glycine max Wm82.a4.v1, and a C / T base substitution occurs at the site. Three KASP primers are developed according to the SNP site, and the primers are shown as SEQ ID NO. 2-4, respectively. The primers are used for PCR amplification and genotyping of a soybean to be tested, and if the detection result shows that the genotyping of the marker position is 1 / 1, it is determined that the petiole angle of the soybean variety is large; if the detection result is 0 / 0, it is determined that the petiole angle is small. The SNP molecular marker can be used for molecular marker assisted selection of the petiole angle trait of soybean, and has important theoretical and practical guiding significance for accelerating genetic improvement of compact plant type of soybean and improving breeding selection efficiency. The marker can quickly identify the petiole angle of soybean, the method is simple and fast, the identification result is accurate, and the marker has a good application prospect.

The application provides a molecular marker and identification method for identifying genuine producing areas and non-genuine producing areas of Astragalus membranaceus. The molecular marker contains a nucleotide sequence with a polymorphism of A / T at the 131st base of the sequence shown in SEQ ID NO: 1 of the 5th exon of Am02G035490 of the Astragalus membranaceus genome. The application also provides a method for identifying the genuine producing areas of Astragalus membranaceus by using the SNP molecular marker, which provides technical support for the precise traceability, stable quality and healthy and orderly development of the Astragalus membranaceus industry of the genuine producing areas of Astragalus membranaceus.

This utility model discloses an integrated emergency lighting device capable of real-time detection of human body location. The device includes an emergency lighting fixture body, which integrates a millimeter-wave radar sensing module, a thermal imaging sensor, a voice recognition module, a CO gas sensor, a PLC control module, a drive unit, and an audible alarm. The millimeter-wave radar sensing module, thermal imaging sensor, voice recognition module, CO gas sensor, drive unit, and audible alarm are all electrically connected to the PLC control module. The emergency lighting fixture body has two evacuation lights, each electrically connected to the PLC control module via a drive unit, forming independent working circuits that do not interfere with each other. This utility model can promptly monitor and locate the position of trapped personnel, providing firefighters with accurate rescue information, improving rescue precision, avoiding blind searches, shortening rescue time, and reducing casualties and property damage.

This invention discloses a functional gene grb Application of recombinant mutant molecular markers in hybrid bream breeding, this functional gene grb Recombinant mutant molecular markers are used to identify hybrid bream germplasm or screen backcross progeny of hybrid bream with fast growth traits, among which functional genes... grb Recombinant variant molecular markers are located in functional genes grb The base mutations at positions 2079, 2083, 2101, and 2161 are as follows: position 2079 is C or A, position 2083 is A or G, position 2101 is C or A, and position 2161 is G or C. This is the functional gene of the present invention. grb Recombinant mutant molecular markers can not only quickly identify the germplasm of hybrid bream, but also accurately screen backcross progeny of hybrid bream with fast growth traits, providing technical support for precision hybridization breeding and providing screening sites for the development of breeding chips.

This invention belongs to the field of biotechnology and discloses a molecular marker Cl_Chr08_5111232 related to watermelon fruit weight and its applications. This invention measures the weight of watermelon fruits at maturity and, by constructing a high-density genetic linkage map, obtains a stable and reliable molecular marker Cl_Chr08_5111232 that is closely linked to watermelon fruit weight. This molecular marker is located at position 5111232 on chromosome 8 of the watermelon reference genome 97103V1, and a marker for SNP detection is developed based on this site. This invention also provides a method for rapidly identifying watermelon fruit weight using primer sets based on the aforementioned molecular marker. This method is simple, fast, and accurate, and has good prospects for widespread application.

The application relates to the field of agricultural biotechnology, and particularly discloses a KASP molecular marker for identifying the resistance of soybeans to phytophthora root rot and application. The KASP molecular marker primer sequence is shown in the upstream primer SEQ ID No. 1 (connected with a FAM fluorescent tag), the upstream primer SEQ ID No. 2 (connected with a VIC fluorescent tag) and the universal downstream primer SEQ ID No. 3. The KASP molecular marker can realize efficient, accurate and low-cost identification and genotyping of the resistance of soybeans to phytophthora root rot, and the effective identification and detection reaches 98.55%, and the accuracy reaches 84.80%.

This application provides a method, apparatus, and system for high-precision assembly quality control of aerospace composite materials, relating to the fields of aerospace manufacturing assembly and intelligent control technology. The method includes: acquiring the initial shape deviation between the current shape of the section to be assembled and the target shape; controlling each actuator in the actuator array to apply test loads in a preset order, recording the displacement data of each actuator, and identifying the local response matrix between the actuators; inputting the local response matrix into a pre-constructed joint Bayesian prior model to calculate the global response matrix of the section to be assembled, and calculating the optimal actuator control force vector based on the initial shape deviation and the global response matrix. The method, apparatus, and system for high-precision assembly quality control of aerospace composite materials provided by this application can fully utilize the sensing function of the actuators to quickly identify the stiffness characteristics of each section without adding additional external measurements, thereby achieving efficient and high-precision shape control.

This invention discloses a method for identifying the stipe length of Ganoderma lucidum using genetic differences, belonging to the field of Ganoderma lucidum breeding and cultivation technology. This method utilizes specific primer pairs del670 or insertion2441 to perform PCR amplification of Ganoderma lucidum mycelial DNA. Early prediction of the stipe phenotype is achieved by detecting the size of the amplified product fragments: if a 1569 bp fragment is obtained using the del670 primer pair or a 1357 bp fragment is obtained using the insertion2441 primer pair, the strain is identified as short-stalked; if an 899 bp fragment is obtained using the del670 primer pair or a 3798 bp fragment is obtained using the insertion2441 primer pair, the strain is identified as long-stalked. This method enables rapid and accurate identification of Ganoderma lucidum stipe length at the mycelial stage, eliminating the need for traditional fruiting cultivation, significantly shortening the breeding cycle and reducing testing costs.

This invention relates to the field of genetic engineering technology, and more particularly to a SNP molecular marker related to wheat appearance quality and its application. The SNP molecular marker described in this invention contains a nucleotide sequence with a polymorphism of C or T at position 767257977 bp on wheat chromosome 2A. This invention has determined that this site is a polymorphic site affecting grain length and protein content, and has designed a specific KASP primer combination to achieve rapid and accurate identification of wheat grain length and protein content.

The application discloses a quadruple RT-qPCR method for simultaneously detecting canine digestive tract viruses, a kit and application, and the real-time fluorescent quantitative PCR detection kit comprises PCR primer probes, the PCR primer probes comprise forward PCR amplification primers, reverse PCR amplification primers and detection probes respectively for canine parvovirus, canine coronavirus, canine adenovirus type 1 and canine rotavirus; the sequences are shown in SEQ ID NO. 1 to SEQ ID NO. 12, respectively, the application can detect canine parvovirus, canine coronavirus, canine adenovirus type 1 and canine rotavirus, has very high sensitivity and specificity, and is favorable for early diagnosis or treatment of sick dogs.

This application relates to a method for detecting paper fibers, comprising the following steps: mixing the paper sample to be tested with a solvent, and dissolving it at a rotation speed of 8000 r / min to 25000 r / min for 1 min to 11 min to obtain a dissolved sample; adding a dye to the dissolved sample to dye the wood and hemp fibers in the dissolved sample to obtain a dyed sample; and performing microscopic detection on the dyed sample to analyze the detection results. Through controllable mechanical shear force, the wood and hemp fibers in the paper sample to be tested can be efficiently and fully dispersed, while minimizing the damage to fiber morphology caused by traditional chemical or high-temperature dissociation methods, laying a physical foundation for subsequent accurate identification. Therefore, this application can rapidly and accurately identify and detect fiber type and proportion through dyeing reaction and microscopic morphology, resulting in more scientific and practical analytical results.

The application discloses an InDel molecular marker for identifying MeiGua single and double petal characters, a primer and application thereof, and comprises two InDel molecular markers, namely InDel 9 located at 5549358~5549382bp of a No.1 chromosome of MeiGua and InDel 90 located at 6309277~6309290bp of the No.1 chromosome of MeiGua. The application also develops a primer for amplifying the two InDel markers. The molecular marker can be used for quickly and efficiently identifying MeiGua single and double petal varieties at a seedling stage of MeiGua, and the accuracy rate reaches 100%. Compared with traditional breeding which needs to wait for about four years for flowering, the marker has the advantages of early time, low cost, accuracy and rapidness when applied to assisted breeding.

This invention belongs to the field of silicon carbide single crystal detection technology, specifically involving a silicon carbide polymorphism identification method and system based on stress distribution characteristics. The method acquires stress distribution data of the entire silicon carbide wafer, identifies at least two statistically stable background stress plateau regions and continuously distributed high-stress bands between them, extracts the mean of the plateau regions and the peak value of the high-stress bands, and uses logical judgment based on the difference and multiple relationship between the two to determine the polymorphism coexistence state. When polymorphism coexistence exists, a local extreme point search is performed on the high-stress bands to generate polymorphism boundaries, and the mean of the plateau regions is compared with a preset polymorphism feature database to output the specific polymorphism type. This invention utilizes the stress increase characteristics at the polymorphism interface corresponding to structural mismatch and stacking sequence abrupt changes to achieve rapid and accurate polymorphism identification and interface localization without point-by-point spectral scanning.