56results about How to "Rapid identification" patented technology

The invention relates to the field of genes, in particular to molecular markers for judging flowering time of brassica napus L. The molecular markers are Indel-282, Indel-326 and Indel-353 respectively and are located on an A02 chromosome of the brassica napus L.. The molecular marker has high specificity and stability, and is significantly related to the flowering time. The markers show high specificity and stability in brassica napus plants under different genetic backgrounds. By detecting the molecular markers through PCR amplification and electrophoresis, the flowering time of different plants can be quickly and accurately identified in the early stage of breeding. Therefore, the flowering time of the plant can be determined in the early stage, so that the breeding efficiency is improved. The molecular markers can be used for molecular marker-assisted selective breeding, and plants with early flowering or late flowering characteristics can be quickly screened by detecting the markers, so that the breeding process of excellent varieties is accelerated, and the functions and regulation mechanisms of related genes are further disclosed.

This invention relates to a method for establishing and detecting the fingerprint spectrum of the traditional Chinese medicine composition Taohongyin. The raw materials of the traditional Chinese medicine composition include: chicken gizzard lining, papaya, hawthorn, chrysanthemum, tangerine peel, peach kernel, and wolfberry. The fingerprint spectrum establishment method includes the preparation of a reference solution and a test solution; detection of the reference solution and the test solution using high-performance liquid chromatography (HPLC); the mobile phase used in the HPLC is acetonitrile and phosphoric acid aqueous solution, and the elution method is gradient elution.

This invention discloses a SNP molecular marker, primer set, kit, and application associated with the low fertility trait of flower bud splitting in mung beans, belonging to the field of plant molecular marker screening and molecular genetic breeding technology. The molecular marker is located at position 43652302 on chromosome 4 of the mung bean genome, on the EVM0022977 gene; the SNP molecular marker is the nucleotide sequence shown in SEQ ID NO.1, where the 120th base is either T or A. The SNP molecular marker and primer set provided by this invention are specific, accurate, reliable, and easy to operate, enabling rapid identification of mung bean flower bud splitting low fertility mutants, meeting the needs of assisted mung bean molecular breeding and shortening the breeding cycle.

This invention discloses an intelligent voiceprint anti-counterfeiting method, device, and equipment based on hops signals. It collects the voiceprint signals generated by hops hitting the bottle wall, then denoises the signals and extracts PLP features. These feature signals are then input into a CNN-GMM-HMM voiceprint model for training to construct an original standard database. After collecting sample voiceprint signals, they are preprocessed and then matched and identified with the voiceprint information in the standard database to achieve authenticity verification. Compared to traditional identification methods, this method can identify genuine and counterfeit liquor faster and more accurately, greatly improving work efficiency while helping to ensure the quality and safety of liquor.

The application provides a construction of a fluorescent protein labeled microtubulin and a microtubule binding protein universal bivalent carrier, and the process is as follows: a first stage is to construct a GFP-alpha tubulin carrier, and a second stage is to construct a GFP-alpha tubulin-mCherry universal bivalent co-expression carrier; in the second stage, 35S, mCherry and NOS sequences are inserted into a KpnI enzyme cutting site of a multiple cloning site of the GFP-alpha tubulin carrier constructed in the first stage, and a single nucleic acid enzyme cutting site is inserted into the 5' end and the 3' end of the mCherry sequence, respectively, and the single nucleic acid enzyme cutting site is XbaI, KpnI / Acc65I and AscI sequences, respectively, so as to obtain the universal bivalent carrier. The universal bivalent carrier provided by the application can express two target genes simultaneously, has the fluorescent signals of GFP and mCherry, can quickly and accurately identify a transgenic plant, is convenient for positive seedling screening, can be used for a tobacco transient expression experiment, can shorten an experimental period, and saves time and effort.

This invention provides a maize plant architecture regulating gene ZmEXO1 and its application, belonging to the field of maize molecular breeding technology. The amino acid sequence of ZmEXO1 is shown in SEQ ID NO.7. Studies have found that ZmEXO1 can regulate maize leaf angle and plant height; mutants with reduced or silenced ZmEXO1 expression lead to smaller leaf angles and reduced plant height. Therefore, gene editing of the ZmEXO1 gene or genetic breeding improvement using Zmexo1 low-expression mutants can obtain maize germplasm with improved plant architecture, which is of great significance for enriching maize germplasm and breeding high-yielding, dense-planting-tolerant varieties. This invention also provides a Kasp molecular marker and Kasp primers closely linked to ZmEXO1, which can be used for marker-assisted selection breeding and the discovery of related functional genes, enabling rapid screening and identification of maize germplasm containing ZmEXO1-modified molecules.

The present application relates to the field of biotechnology, and more particularly to a mylopharyngodon piceus gender-specific molecular marker, primer and application thereof, wherein the primer designed for the molecular marker is used for PCR amplification, and the genetic sex of the mylopharyngodon piceus can be accurately and rapidly identified. The molecular marker presents stable difference between male and female individuals, and the identification accuracy is 100%. In addition, the molecular marker can be stably applied to mylopharyngodon piceus of different specifications and different breeding strains, and shows good universality and reliability. The present application provides important technical support for the unisexual breeding, germplasm resource management and population structure monitoring of mylopharyngodon piceus, and has wide application prospect.

This invention provides the application of phosphatidylinositol (16:0 / 18:3(9 cis, 12 cis, 15 cis)) in the identification of Hainan camellia, relating to the field of food testing technology. The phosphatidylinositol (16:0 / 18:3(9 cis, 12 cis, 15 cis)) can be used as a characteristic marker for the identification of Hainan camellia. This invention is the first to discover that the kernels of Hainan camellia contain high levels of phosphatidylinositol (16:0 / 18:3(9 cis, 12 cis, 15 cis)), while phosphatidylinositol (16:0 / 18:3(9 cis, 12 cis, 15 cis)) is not found or present in extremely low amounts in the kernels of other camellia species besides Hainan camellia. Phosphatidylinositol (16:0 / 18:3(9 cis, 12 cis, 15 cis)) can be used to identify and distinguish Hainan camellia from other camellia species. Furthermore, this identification method is simple to operate and achieves rapid, objective, and accurate identification of Hainan camellia.

The application discloses a primer pair, a kit and a method for detecting the expression amount of a liver function gene HNF1A. The primer pair comprises a forward primer as shown in SEQ ID NO:1 and a reverse primer as shown in SEQ ID NO:2. The primer pair of the application can realize monitoring of the relative expression amount of the HNF1A gene in a liver function cell product, and can realize rapid identification of liver function cells and relative expression of the HNF1A function gene of the cells, and the method is simple and convenient to operate.

The application discloses a SSR molecular marker primer related to Chinese chives dormancy and a detection method and application thereof. In view of the problems that traditional Chinese chives dormancy trait identification relies on field morphological observation for 4 months, is inefficient and lacks special molecular markers, 7 specific SSR primers (SSR005 / 170 / 249 / 325 / 423 / 478 / 610) are developed based on cross-species genome resequencing (taking Chinese onion as a reference genome, and the alignment rate is 18.76%-18.89%) for the first time. Through the establishment of a matching detection system: CTAB method for rapid DNA extraction, optimization of the PCR system (20 muL contains 30 ng of template DNA, 0.4 U of Taq enzyme, 0.2 muM of primer, 0.2 mM of Mg 2+ 2.0 mM of dNTPs, 57 DEG C annealing), 8% polyacrylamide gel electrophoresis combined with silver staining development, the dormancy trait can be accurately identified within 7 days. For example, the SSR423 primer has no band at 177 bp, and the non-dormant / dormant germplasm cannot be distinguished. The detection results of 52 germplasms (such as dormancy germplasm 21-1 and non-dormancy germplasm 22-7) are consistent with the field phenotypes at a rate of 100%.

This utility model discloses a burr detection device for longitudinal shearing of silicon steel coils, belonging to the field of burr detection technology. It includes a trolley with a weighing and unwinding mechanism on its top, on which a longitudinally sheared silicon steel coil is mounted. A limiting mechanism is located on the side of the trolley, and a supporting mechanism is mounted on the limiting mechanism. A lifting box is fixedly connected to the top surface of the trolley, and a screw is rotatably connected to the inner cavity of the lifting box. A lifting seat is threaded onto the outer side of the screw. In this utility model, two stroboscopes capture images of the top and bottom of the released silicon steel sheet, and the captured images are transmitted to an external monitoring device. Under the action of the stroboscopes, the silicon steel sheet appears to be in a static state, thus allowing the monitoring device to clearly display defects. Even minute defects can be quickly identified, greatly improving production efficiency and reducing safety risks.

The invention discloses a Sorosil decoction specific chromatogram and a construction method and application thereof, and belongs to the technical field of traditional Chinese medicine analysis and detection. The method comprises the following steps: respectively dissolving reference substances of bergenin, arbutin, glycyrrhizic acid, liquiritin, liquiritigenin and gallic acid in a solvent to obtain reference substance solutions; adding soroxil decoction into a solvent, carrying out ultrasonic extraction, and filtering to obtain a test solution; and injecting the reference solution and the test solution into a high performance liquid chromatograph for detection, taking the specific chromatogram of the reference solution as a reference chromatogram, selecting common peaks from the specific chromatogram of the test solution, and constructing the specific chromatogram of the soroxil decoction. The invention also discloses a Sorosil decoction characteristic spectrum and application thereof. According to the specific chromatogram of the soroxi decoction, rapid identification of the formula of the soroxi decoction can be realized, the method is simple, the research blank of the specific chromatogram in quality control of the classic famous-prescription soroxi decoction and the preparation thereof is filled up, and a powerful guarantee is provided for quality improvement of the classic famous-prescription soroxi decoction.

The utility model discloses a kind of intelligent key cabinet with display screen, comprising: cabinet, intelligent control panel and key management kit, key management kit includes: key management cavity, door plate, electric lock, RFID sensing module and electronic display screen, and the key stored in key management kit is attached with RFID chip.On the key of key management kit, RFID chip is detected and identified by RFID sensing module, and detection information is fed back to intelligent control panel, and the vehicle code corresponding to key is displayed on the corresponding electronic display screen, so that the vehicle information corresponding to the key stored in key management kit is conveniently and quickly identified. Before user needs to take the vehicle corresponding key, whether the required vehicle key has been lent out can be identified by the information displayed on electronic display screen, so as to improve the efficiency of key lending and returning, and facilitate the storage and lending management of vehicle key.

The application discloses a specific primer pair for detecting Coccidioides immitis and application thereof, and belongs to the technical field of microorganism detection. Six groups of forward primers and reverse primers are disclosed, and the specific primer pair of any group can be used to accurately identify Coccidioides immitis. The detection method is rapid, specific, convenient to operate and accurate in identification.

The application discloses a detection method of Baizhu San standard decoction fingerprint combined with liquid chromatography-mass spectrometry, the fingerprint and application thereof, and belongs to the field of traditional Chinese medicine quality standard research and application. Test sample solution and single control sample solution are injected into a high performance liquid chromatograph to obtain respective chromatograms; mass spectrometry analysis is conducted on the test sample solution to obtain mass spectrometry results; common peaks existing in the chromatogram of the test sample solution are taken as common peaks, chemical components in the Baizhu San standard decoction are identified in combination with the chromatogram of the single control sample solution and the mass spectrometry results of the test sample solution, and the Baizhu San standard decoction fingerprint is obtained. The fingerprint is constructed through liquid chromatography-mass spectrometry, 10 intersecting components are identified in combination with the mass spectrometry information of the control sample, so that the quality control of the Baizhu San standard decoction is realized, the method is simple, easy to operate, high in precision, good in stability and reproducibility, and can effectively solve the technical problem that the quality of the Baizhu San cannot be effectively evaluated.

The invention belongs to the technical field of identification of variety resources and germplasm innovation, and discloses an SSR (simple sequence repeat) core primer combination for identifying the content of'high saikoside 'in a radix bupleuri variety, a kit and application. According to the invention, a pair of specific SSR core primers (CHSSR1F / CHSSR1R) are screened by using a microsatellite molecular marker development technology. The primer combination can specifically amplify a characteristic band of 136 bp in a Chinese thorowax root variety with high saikosaponin, so that the Chinese thorowax root variety with low saikosaponin can be quickly and accurately identified and distinguished from other Chinese thorowax root varieties with low saikosaponin. The SSR core primer combination, the kit and the identification method provided by the invention overcome the defects that the traditional chromatographic identification method is time-consuming, high in cost, dependent on equipment and the like, have the advantages of reliable result, simplicity and convenience in operation, high detection efficiency and the like, and are beneficial to popularization and protection of a'high saikosaponin 'radix bupleuri variety and medicinal material quality control.

The application belongs to the technical field of soybean molecular genetics and breeding, and discloses a KASP marker for detecting a SNP site related to a soybean petiole angle and application thereof. The SNP site is located at position 4589285 on chromosome 10 of a soybean reference genome Glycine max Wm82.a4.v1, and a C / T base substitution occurs at the site. Three KASP primers are developed according to the SNP site, and the primers are shown as SEQ ID NO. 2-4, respectively. The primers are used for PCR amplification and genotyping of a soybean to be tested, and if the detection result shows that the genotyping of the marker position is 1 / 1, it is determined that the petiole angle of the soybean variety is large; if the detection result is 0 / 0, it is determined that the petiole angle is small. The SNP molecular marker can be used for molecular marker assisted selection of the petiole angle trait of soybean, and has important theoretical and practical guiding significance for accelerating genetic improvement of compact plant type of soybean and improving breeding selection efficiency. The marker can quickly identify the petiole angle of soybean, the method is simple and fast, the identification result is accurate, and the marker has a good application prospect.

The invention discloses a primer group and a kit for identifying varieties of cabbage mustard and application of the primer group and the kit. The primer group for identifying the variety of the cabbage mustard comprises a first primer pair to a 500th primer pair, forward primers of the first primer pair to the 500th primer pair are sequentially shown as SEQ ID NO.1 to 500, reverse primers of the first primer pair to the 500th primer pair are sequentially shown as SEQ ID NO.501 to 1000, namely, the forward primer and the reverse primer of the first primer pair are respectively shown as SEQ ID NO.1 and 501, and so on. The primer group disclosed by the invention is used for carrying out PCR amplification and sequencing on a to-be-detected sample to obtain DNA fingerprint data of the to-be-detected sample, and comparing the DNA fingerprint data with DNA fingerprint data of cabbage mustard of a known variety to obtain a variety identification result of the to-be-detected sample; when the primer group is used for identifying the variety of the cabbage mustard, the resolution reaches a single-base level, the accuracy and the digitization degree are high, the variety of the cabbage mustard can be identified quickly at high throughput, and the accuracy and the efficiency of identifying the variety of the cabbage mustard are remarkably improved.

The application discloses an anti-MxA antibody or a functional fragment thereof and application thereof. The anti-MxA antibody or the functional fragment thereof disclosed by the application comprises a heavy chain complementarity determining region and a light chain complementarity determining region, has good specificity and sensitivity to MxA, and can be used for detecting MxA and diagnosing or assisting in diagnosing related diseases taking MxA as a marker, and has important clinical value.

The application provides a molecular marker and identification method for identifying genuine producing areas and non-genuine producing areas of Astragalus membranaceus. The molecular marker contains a nucleotide sequence with a polymorphism of A / T at the 131st base of the sequence shown in SEQ ID NO: 1 of the 5th exon of Am02G035490 of the Astragalus membranaceus genome. The application also provides a method for identifying the genuine producing areas of Astragalus membranaceus by using the SNP molecular marker, which provides technical support for the precise traceability, stable quality and healthy and orderly development of the Astragalus membranaceus industry of the genuine producing areas of Astragalus membranaceus.

This invention discloses a quinoa SSR molecular marker primer set and its application in DNA fingerprinting, belonging to the field of molecular biology. This invention developed a quinoa SSR molecular marker primer set consisting of 25 pairs of SSR primers, the nucleotide sequences of which are shown in SEQ ID NO. 1-50. This invention also used this primer set to perform genetic diversity analysis and population genetic structure assessment on 170 quinoa germplasm resources, further screening out 18 core primer pairs, and using these core primers to construct a quinoa DNA fingerprint. The results show that the quinoa DNA fingerprint can effectively characterize quinoa varieties, simultaneously identify multiple quinoa varieties, and the identification method is convenient, rapid, accurate, and stable. This invention provides reliable theoretical and technical support for the precise identification and innovation of quinoa germplasm resources, the breeding of new quinoa varieties, and the protection of variety rights.

This utility model discloses an integrated emergency lighting device capable of real-time detection of human body location. The device includes an emergency lighting fixture body, which integrates a millimeter-wave radar sensing module, a thermal imaging sensor, a voice recognition module, a CO gas sensor, a PLC control module, a drive unit, and an audible alarm. The millimeter-wave radar sensing module, thermal imaging sensor, voice recognition module, CO gas sensor, drive unit, and audible alarm are all electrically connected to the PLC control module. The emergency lighting fixture body has two evacuation lights, each electrically connected to the PLC control module via a drive unit, forming independent working circuits that do not interfere with each other. This utility model can promptly monitor and locate the position of trapped personnel, providing firefighters with accurate rescue information, improving rescue precision, avoiding blind searches, shortening rescue time, and reducing casualties and property damage.

The invention belongs to the field of cassava molecular markers, and particularly relates to a cassava KASP marker and application of the cassava KASP marker in cassava variety distinguishing. The 27 KASP markers provided by the invention have good distinguishing ability in cassava genetic analysis, and can effectively reveal genetic differences among different materials. The DNA fingerprint spectrum of 184 parts of cassava materials is constructed based on 27 core KASP markers, and the purpose of rapid variety identification can be achieved. The KASP marker disclosed by the invention can be used for effectively identifying false selfing offspring. In conclusion, the KASP marker provided by the invention provides a new method and thought for genetic improvement and variety identification of cassava, provides a powerful tool for subsequent cassava breeding and genetic improvement research, and is beneficial to breeding of new varieties with excellent characters.

This invention discloses a functional gene grb Application of recombinant mutant molecular markers in hybrid bream breeding, this functional gene grb Recombinant mutant molecular markers are used to identify hybrid bream germplasm or screen backcross progeny of hybrid bream with fast growth traits, among which functional genes... grb Recombinant variant molecular markers are located in functional genes grb The base mutations at positions 2079, 2083, 2101, and 2161 are as follows: position 2079 is C or A, position 2083 is A or G, position 2101 is C or A, and position 2161 is G or C. This is the functional gene of the present invention. grb Recombinant mutant molecular markers can not only quickly identify the germplasm of hybrid bream, but also accurately screen backcross progeny of hybrid bream with fast growth traits, providing technical support for precision hybridization breeding and providing screening sites for the development of breeding chips.

The invention belongs to the technical field of vegetable planting, and discloses vegetable planting equipment which comprises a supporting frame, a telescopic supporting pipe is fixedly installed in an inner cavity of the supporting frame, a conveying pump is fixedly connected to the left side of the telescopic supporting pipe, and a nutrient solution pipe is fixedly connected to the right side of the telescopic supporting pipe. A spray nozzle is fixedly connected to the front side of the nutrient solution pipe, an air bag is fixedly installed in an inner cavity of the nutrient solution pipe, a rotating rod is fixedly connected to the bottom end of the nutrient solution pipe, a servo motor is fixedly connected to the right side of the supporting frame, and an output shaft of the servo motor is fixedly sleeved with a ball screw; the surface of the ball screw is engaged with a moving frame, and the left side of the moving frame is fixedly connected with a coil spring barrel. Therefore, when the nutrient solution is sprayed to the dysplastic vegetables, the spraying angle of the nozzle can be changed, and more nutrient solution can be sprayed.

This patent discloses a method for early warning of mold growth in cigar tobacco leaves using Aspergillus montmorillonite. The method includes dynamically detecting the Aspergillus montmorillonite content in cigar tobacco leaves using a reagent kit (Aspergillus montmorillonite 4.4-A, accession number CCTCC NO: M2025990); classifying the dynamically detected Aspergillus montmorillonite content using a central processing unit (CPU); establishing a cigar mold growth equation to calculate the probability of mold growth in the cigar tobacco leaves; and correlating the probability of mold growth with the classification. Under different classification standards, the CPU performs one or more combinations of system recording, alert linkage, or automatic processing to achieve early warning of mold growth in cigar tobacco leaves. This provides sufficient time for mold prevention in cigar tobacco leaves, avoiding irreversible losses due to excessively long detection times and improving the usability of the cigar tobacco leaves.

The invention belongs to the technical field of molecular breeding of vegetables, and particularly relates to an InDel molecular marker co-segregated with the length of a fruit stem of a bitter gourd and application of the InDel molecular marker. The mutation site of the InDel molecular marker co-separated from the length of the fruit stem of the bitter gourd corresponds to the insertion / deletion of TTTTAAAAAAT at the 14693294bp on the No.3 chromosome of a bitter gourd genome, and the polymorphism of the base at the site affects the length of the fruit stem of the bitter gourd. The invention also provides a primer pair, a kit and the like for identifying the InDel molecular marker, which can be used for molecular marker-assisted selective breeding of the length of the fruit stem of the bitter gourd, improve the efficiency of genetic improvement of the length of the fruit stem of commercial fruit of the bitter gourd, shorten the breeding period and reduce the breeding cost, and has a wide application prospect in practice of the length of the fruit stem of the bitter gourd.

This invention belongs to the field of biotechnology and discloses a molecular marker Cl_Chr08_5111232 related to watermelon fruit weight and its applications. This invention measures the weight of watermelon fruits at maturity and, by constructing a high-density genetic linkage map, obtains a stable and reliable molecular marker Cl_Chr08_5111232 that is closely linked to watermelon fruit weight. This molecular marker is located at position 5111232 on chromosome 8 of the watermelon reference genome 97103V1, and a marker for SNP detection is developed based on this site. This invention also provides a method for rapidly identifying watermelon fruit weight using primer sets based on the aforementioned molecular marker. This method is simple, fast, and accurate, and has good prospects for widespread application.