110results about How to "Rapid identification" patented technology

The invention provides a primer for identifying tilapia mossambica as well as a detection kit and application thereof, and belongs to the technical field of molecular biology. The invention provides a primer for identifying the variety of tilapia mossambica, which can be used for quickly, efficiently and accurately identifying Oreochromis niloticus, Oreochromis aurea and Oreochromis spp., so that a scientific basis is provided for germplasm resource protection and variety identification of the tilapia mossambica.

The invention discloses an Indel molecular marker for identifying ricefield eel strains in two Guangdong regions. The nucleotide sequence of the Indel molecular marker A is shown as SEQ ID NO: 5 or SEQ ID NO: 7; the nucleotide sequence of the Indel marker B is as shown in SEQ ID NO: 6 or SEQ ID NO: 8. The invention also provides a primer pair for amplifying the Indel molecular marker. By adopting the Indel molecular marker primer pair provided by the invention to amplify genome DNA of ricefield eel strains in different regions, the ricefield eel strains in the two regions can be quickly screened out according to amplified fragments. The Indel marker provided by the invention can be applied to rapid identification of germplasm resources of a specific geographical strain so as to obtain excellent characters of the strain and finally realize precise breeding.

The invention relates to the technical field of microbial identification, in particular to an identification method of a streptococcus thermophilus CICC 6063 strain and development and application of a strain level primer of the streptococcus thermophilus CICC 6063 strain. The invention provides an application of a nucleotide sequence as shown in SEQ ID NO: 1 in identification of a streptococcus thermophilus CICC 6063 strain. The invention provides an identification method of a streptococcus thermophilus CICC 6063 strain. The method comprises the following steps: extracting genome DNA of a to-be-detected strain, designing a primer aiming at a nucleotide sequence shown in SEQ ID NO: 1, amplifying the genome DNA, and judging whether the to-be-detected strain is the streptococcus thermophilus CICC 6063 strain or not by comparing the length of an actual amplification product with the length of a target amplification product and the condition of polymorphic site basic groups. The invention provides a simple and rapid method for identifying a streptococcus thermophilus CICC 6063 strain by utilizing a specific conserved gene sequence of streptococcus. The primer pair provided by the invention can be used for rapidly and accurately identifying the CICC 6063 strain on the strain level.

The invention discloses a canopy leaf vertical distribution index construction method, and relates to the field of crop monitoring, and the method comprises the steps: S1, manually measuring the plant height (PH) of each cell of corn, dividing the plant height into five layers according to the PH, and obtaining LAI values of the whole canopy and leaves of each layer; s2, fitting cumulative LAI values of different leaf layers by using a Logistic equation; s3, calculating a first-order derivative of the fitted curve to obtain a canopy LAD distribution curve; and S4, extracting the kurtosis (Kurtosis) index and the skewness (Skewness) index of the LAD curve. Meanwhile, a rapid and accurate estimation method for canopy configuration indexes is provided. The method has the advantages that a novel canopy leaf vertical distribution index is constructed according to accumulated LAI values of different heights; and fusing unmanned aerial vehicle multispectral, RGB and LIDAR data, estimating canopy LAI, plant height and constructed indexes, and realizing rapid identification of the corn plant type.

The invention provides a larimichthys crocea fast-growing character associated SNP marker combination and breeding application, the combination is prepared from a probe for detecting 10 SNP molecular marker combinations of larimichthys crocea fast-growing characters, and information of positions of all SNP molecular markers on chromosomes, alleles with changed sites and reference genes is shown in a table 1. According to the SNP marker combination disclosed by the invention, the optimal large yellow croaker individuals with fast-growing potential and a certain scale can be selected from the breeding offspring in advance for breeding and breed conservation, and the large yellow croaker individuals are bred to the parent without breeding and breed conservation of a large number of offspring seeds in the breeding process, so that the breeding cost can be greatly reduced. The SNP marker combination provided by the invention can solve the problem that non-lethal detection of the growth speed of the large yellow croaker cannot be realized in a traditional breeding mode, and can ensure smooth development of fast-growing character breeding work of the large yellow croaker.

The invention discloses a method and a system for rapidly identifying a photo-thermo-sensitive sterile line of forage grass sorghum, and relates to the technical field of agricultural remote sensing. The method comprises the following specific steps: taking a suspected photo-thermo-sensitive sterile line as a material to be detected; acquiring hyperspectral data of the to-be-detected material after heading and before pollen formation by using a hyperspectral imaging technology; extracting identification characteristics related to the photo-thermo-sensitive sterile line of the forage grass sorghum from the hyperspectral data; and establishing an identification model based on the identification characteristics, and inputting a to-be-detected sample into the identification model for rapid identification of the photo-thermo-sensitive sterile line. The method is based on a hyperspectral imaging technology and is combined with a machine learning algorithm to realize rapid, lossless, visual and accurate identification of the photo-thermo-sensitive sterile line of the forage grass sorghum, and the method can greatly improve the identification efficiency and accuracy.

The invention belongs to the technical field of biology, and relates to a barley germination period salt-tolerant gene HvHCT1, a protein and an application of the protein in salt damage stress resistance, and the protein comprises an amino acid sequence shown as SEQ ID NO: 2. According to the method, genetic loci related to the salt tolerance of the barley in the germination period are mined through a whole genome association analysis method, then transcriptome sequencing is used for revealing barley salt tolerance differential gene expression changes after salt damage stress, and in combination with association analysis and transcriptome sequencing data, salt tolerance candidate genes in the germination period of the barley are determined. The transgenic arabidopsis thaliana is used for verification; the research provides an excellent germplasm material and a new salt-tolerant gene for barley salt-tolerant breeding research, and also provides a theoretical basis for barley salt-tolerant molecular marker-assisted breeding in the future.

The invention discloses a lithium battery combined health state estimation and service life prediction method, which comprises the following steps of: 1, predicting the health state and the residual service life of a lithium battery by carrying out pulse test on the lithium battery and establishing a Thevenin model at the aging normal operation stage of the lithium battery; 2, after the lithium battery enters a deep aging stage, performing an EIS test on the lithium battery to obtain impedance spectrum data, and correcting the Thevenin model into a variable order RC model; and 3, estimating the health state of the deep aging stage by using the impedance spectrum data and parameters identified by the variable order RC model, and when the prediction error of the two parameters is smaller than an error threshold value, considering that a battery health state and residual life prediction model suitable for the deep aging stage is successfully established. Otherwise, adjusting the order of the variable-order RC model to reduce the error. According to the invention, the reliability of lithium battery life prediction and estimation in a complete life cycle can be ensured, so that the control complexity and cost of a test working condition can be effectively reduced.

The invention discloses a power grid operation weak point identification method and system based on a transmission and distribution network topological structure, and relates to the technical field of power grid operation, and the method effectively reduces the calculation cost, reduces the model complexity through a graph theory and a network simplification technology, optimizes the use of calculation resources, improves the data processing efficiency, and reduces the calculation cost. Therefore, accurate identification under limited calculation performance is realized; data quality indexes are read and evaluated, and data enhancement and dimensionless processing are performed, so that the integrity and accuracy of data are improved, and the stability of the system during data quality fluctuation is ensured; a deep learning algorithm is adopted, a model training process is optimized through cross validation and hyper-parameter tuning, and supervised learning is performed by using fault and operation historical data, so that dependence on a large amount of labeled data and computing resources is reduced, and efficient and accurate power grid operation weak point identification is realized.

The present invention relates to the technical field of silicon wafer detection, and specifically to a multi-angle visual inspection jig for surface defects of semiconductor silicon wafers, comprising an integral external mechanism, wherein the integral external mechanism also comprises an integral bottom plate. The present invention utilizes the characteristic that light energy can be refracted through a mirror surface. After the semiconductor silicon wafer is processed, the surface of the processed original part has mirror characteristics. At this time, the processed original part is irradiated by an external irradiation mechanism, and the light reaches a photosensitive area along a set light path. When dust exists on the surface of the processed original part or the polishing effect is poor during grinding, the refraction efficiency will be low, and the refracted light projected into the photosensitive area will be reduced. The product avoids the trouble of an operator having to hold the silicon wafer by hand for inspection, reduces the chance of the semiconductor silicon wafer coming into contact with external objects, and avoids collisions between the processed original part and other equipment.

The invention relates to the field of genes, in particular to molecular markers for judging flowering time of brassica napus L. The molecular markers are Indel-282, Indel-326 and Indel-353 respectively and are located on an A02 chromosome of the brassica napus L.. The molecular marker has high specificity and stability, and is significantly related to the flowering time. The markers show high specificity and stability in brassica napus plants under different genetic backgrounds. By detecting the molecular markers through PCR amplification and electrophoresis, the flowering time of different plants can be quickly and accurately identified in the early stage of breeding. Therefore, the flowering time of the plant can be determined in the early stage, so that the breeding efficiency is improved. The molecular markers can be used for molecular marker-assisted selective breeding, and plants with early flowering or late flowering characteristics can be quickly screened by detecting the markers, so that the breeding process of excellent varieties is accelerated, and the functions and regulation mechanisms of related genes are further disclosed.

The invention discloses a public health real information evaluation and early warning system based on an AI technology, and relates to the field of artificial intelligence technology application, and the system comprises a database module which is used for storing information collected by a collection module and providing powerful support for subsequent model construction and real-time response, and a data collection module which is used for collecting real-time information of public health. The invention discloses a public health data collection and processing method, which is used for collecting data related to public health from various sources (such as news, social media, professional forums and the like), including multi-modal data such as texts, pictures, videos and the like, and ensuring that the data sources cover data sources of a knowledge graph and data sources of a false information base. The information processing efficiency is greatly improved through a knowledge graph and advanced model construction, the system has strong adaptive capacity for novel false information, the system can rapidly learn and recognize a new false information mode through fusion of the knowledge graph and a large model, and the risk caused by the false information is effectively reduced.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the tolerance character of white spot syndrome virus (WSSV) of procambarus clarkia, the molecular marker is located in the third intron region of a TRIM gene, is specifically located at 585bp of a DNA (Deoxyribose Nucleic Acid) sequence as shown in SEQ ID NO.1, and is Tgt; the T / C heterozygous genotype is obviously related to the WSSV tolerance character of the procambarus clarkia. The invention provides a favorable marker for breeding of disease-resistant molecules of procambarus clarkii, and can accelerate the breeding process of disease-resistant varieties of procambarus clarkii.

The invention discloses a probe primer combination and a kit for rapidly detecting five kinds of diarrheogenic Escherichia coli. The method comprises the following steps: respectively designing a group of specific primers and Taqman probes according to escV, stx1, stx2 and bfp genes of EPEC and STEC / EHEC, an ipaH gene of EIEC, lt, stp and sth genes of ETEC, and aggR, pic and astA genes of EAEC; the kit can be used for detecting various diarrheogenic Escherichia coli in a single tube at the same time, or can be used for detecting five kinds of diarrheogenic Escherichia coli in different reaction tubes within 30 minutes at the soonest, can meet higher sensitivity, and has definite clinical significance and potential application value.

The invention discloses a customer transaction service visual monitoring method, which comprises a transaction channel, a transaction log database and a front-end monitoring interface which are communicated by adopting a network, log data of customer service transactions are obtained through the transaction channel and are stored in the transaction log database; data needing to be monitored are integrated in a data integration layer of the transaction log database according to a data model, the data are calculated and processed, transaction data are transmitted to a front-end monitoring interface through an API interface, and the front-end monitoring interface presents the current customer transaction situation through a graph; the transaction log database is stored in the local server and / or the cloud server; and the rear end of the front-end monitoring interface is deployed on a local server, so that real-time monitoring of customer transactions can be realized, monitoring personnel can conveniently and quickly distinguish problem sources and make responses, support guarantee is provided for quick operation and maintenance, and the overall operation capability of services is improved.

This invention relates to a method for establishing and detecting the fingerprint spectrum of the traditional Chinese medicine composition Taohongyin. The raw materials of the traditional Chinese medicine composition include: chicken gizzard lining, papaya, hawthorn, chrysanthemum, tangerine peel, peach kernel, and wolfberry. The fingerprint spectrum establishment method includes the preparation of a reference solution and a test solution; detection of the reference solution and the test solution using high-performance liquid chromatography (HPLC); the mobile phase used in the HPLC is acetonitrile and phosphoric acid aqueous solution, and the elution method is gradient elution.

The invention relates to the technical field of winter bamboo shoot detection, and discloses a winter bamboo shoot detection method and system based on electromagnetic induction, and the method comprises the steps: obtaining an area map and a driving path; according to the regional map and the driving path, the ground penetrating radar performs detection in sequence along the driving path to obtain at least one detection image; judging whether a preset feature exists in each detection image or not; when the preset features do not exist in the detection images, executing the step of enabling the ground penetrating radar to perform detection in sequence along the driving path according to the area map and the driving path to obtain the detection images; when the preset features exist in any detection image, determining the detection point corresponding to the detection image with the preset features as an operation position; and a preset mark is drawn on the operation position through the marking mechanism. The underground winter bamboo shoots are identified, then the marking mechanism draws the operation positions corresponding to the detection images, the underground positions of the winter bamboo shoots are rapidly identified, and the collection efficiency of the winter bamboo shoots is effectively improved.

This invention discloses a SNP molecular marker, primer set, kit, and application associated with the low fertility trait of flower bud splitting in mung beans, belonging to the field of plant molecular marker screening and molecular genetic breeding technology. The molecular marker is located at position 43652302 on chromosome 4 of the mung bean genome, on the EVM0022977 gene; the SNP molecular marker is the nucleotide sequence shown in SEQ ID NO.1, where the 120th base is either T or A. The SNP molecular marker and primer set provided by this invention are specific, accurate, reliable, and easy to operate, enabling rapid identification of mung bean flower bud splitting low fertility mutants, meeting the needs of assisted mung bean molecular breeding and shortening the breeding cycle.

This invention discloses an intelligent voiceprint anti-counterfeiting method, device, and equipment based on hops signals. It collects the voiceprint signals generated by hops hitting the bottle wall, then denoises the signals and extracts PLP features. These feature signals are then input into a CNN-GMM-HMM voiceprint model for training to construct an original standard database. After collecting sample voiceprint signals, they are preprocessed and then matched and identified with the voiceprint information in the standard database to achieve authenticity verification. Compared to traditional identification methods, this method can identify genuine and counterfeit liquor faster and more accurately, greatly improving work efficiency while helping to ensure the quality and safety of liquor.

The application provides a construction of a fluorescent protein labeled microtubulin and a microtubule binding protein universal bivalent carrier, and the process is as follows: a first stage is to construct a GFP-alpha tubulin carrier, and a second stage is to construct a GFP-alpha tubulin-mCherry universal bivalent co-expression carrier; in the second stage, 35S, mCherry and NOS sequences are inserted into a KpnI enzyme cutting site of a multiple cloning site of the GFP-alpha tubulin carrier constructed in the first stage, and a single nucleic acid enzyme cutting site is inserted into the 5' end and the 3' end of the mCherry sequence, respectively, and the single nucleic acid enzyme cutting site is XbaI, KpnI / Acc65I and AscI sequences, respectively, so as to obtain the universal bivalent carrier. The universal bivalent carrier provided by the application can express two target genes simultaneously, has the fluorescent signals of GFP and mCherry, can quickly and accurately identify a transgenic plant, is convenient for positive seedling screening, can be used for a tobacco transient expression experiment, can shorten an experimental period, and saves time and effort.

The invention relates to the technical field of pathogen detection, and discloses a kit for rapid detection and simultaneous identification of Zika virus and Japanese encephalitis virus by a one-tube method and an application thereof. The kit comprises an enzyme dry powder group, an RPA dual amplification system and a dual-target nucleic acid test strip, wherein the RPA dual amplification system comprises a ZIKV-F / ZIKV-R primer pair, a JEV-F / JEV-R primer pair, a ZIKV-Pb probe and a JEV-Pb probe; the ZIKV-F / ZIKV-R primer pair comprises SEQ ID NO.1 and SEQ ID NO.2; the JEV-F / JEV-R primer pair comprises SEQ ID NO.4 and SEQ ID NO.5; the ZIKV-Pb probe comprises SEQ ID NO.3; and the JEV-Pb probe comprises SEQ ID NO.6. This scheme designs specific primer pairs and probes for the genomic RNA of the two viruses, and modifies the primers / probes of the two viruses with different positioning antigen molecules and color antigen molecules, so as to facilitate the rapid and simultaneous detection of whether the sample contains one or both of the two viruses, thereby achieving simultaneous detection of the two viruses.

This invention provides a maize plant architecture regulating gene ZmEXO1 and its application, belonging to the field of maize molecular breeding technology. The amino acid sequence of ZmEXO1 is shown in SEQ ID NO.7. Studies have found that ZmEXO1 can regulate maize leaf angle and plant height; mutants with reduced or silenced ZmEXO1 expression lead to smaller leaf angles and reduced plant height. Therefore, gene editing of the ZmEXO1 gene or genetic breeding improvement using Zmexo1 low-expression mutants can obtain maize germplasm with improved plant architecture, which is of great significance for enriching maize germplasm and breeding high-yielding, dense-planting-tolerant varieties. This invention also provides a Kasp molecular marker and Kasp primers closely linked to ZmEXO1, which can be used for marker-assisted selection breeding and the discovery of related functional genes, enabling rapid screening and identification of maize germplasm containing ZmEXO1-modified molecules.

The present application relates to the field of biotechnology, and more particularly to a mylopharyngodon piceus gender-specific molecular marker, primer and application thereof, wherein the primer designed for the molecular marker is used for PCR amplification, and the genetic sex of the mylopharyngodon piceus can be accurately and rapidly identified. The molecular marker presents stable difference between male and female individuals, and the identification accuracy is 100%. In addition, the molecular marker can be stably applied to mylopharyngodon piceus of different specifications and different breeding strains, and shows good universality and reliability. The present application provides important technical support for the unisexual breeding, germplasm resource management and population structure monitoring of mylopharyngodon piceus, and has wide application prospect.

This invention provides the application of phosphatidylinositol (16:0 / 18:3(9 cis, 12 cis, 15 cis)) in the identification of Hainan camellia, relating to the field of food testing technology. The phosphatidylinositol (16:0 / 18:3(9 cis, 12 cis, 15 cis)) can be used as a characteristic marker for the identification of Hainan camellia. This invention is the first to discover that the kernels of Hainan camellia contain high levels of phosphatidylinositol (16:0 / 18:3(9 cis, 12 cis, 15 cis)), while phosphatidylinositol (16:0 / 18:3(9 cis, 12 cis, 15 cis)) is not found or present in extremely low amounts in the kernels of other camellia species besides Hainan camellia. Phosphatidylinositol (16:0 / 18:3(9 cis, 12 cis, 15 cis)) can be used to identify and distinguish Hainan camellia from other camellia species. Furthermore, this identification method is simple to operate and achieves rapid, objective, and accurate identification of Hainan camellia.

The present invention discloses a quality detection method for goat milk leaf medicinal materials, including identification and / or content determination; the identification of goat milk leaf medicinal materials is a thin layer chromatography identification method; the content determination is to determine the content of silver forging glycosides in goat milk leaf medicinal materials by high performance liquid chromatography. The thin layer identification method of goat milk leaf medicinal materials adopted in the present application has the characteristics of high sensitivity, strong specificity and good separation effect, is simple and feasible, has strong reproducibility, and can accurately identify the authenticity of medicinal materials; the method for determining the content of silver forging glycosides in goat milk leaf medicinal materials adopted in the present application can accurately and quickly determine the content of goat milk leaf medicinal materials, and provide effective qualitative and quantitative analysis means for goat milk leaf medicinal materials, so as to facilitate the quality control of goat milk leaf medicinal materials. The quality detection method of goat milk leaf medicinal materials provided by the present invention can effectively control the overall quality of goat milk leaf medicinal materials and ensure the safety and effectiveness of goat milk leaf medicinal materials in clinical use.

The application discloses a primer pair, a kit and a method for detecting the expression amount of a liver function gene HNF1A. The primer pair comprises a forward primer as shown in SEQ ID NO:1 and a reverse primer as shown in SEQ ID NO:2. The primer pair of the application can realize monitoring of the relative expression amount of the HNF1A gene in a liver function cell product, and can realize rapid identification of liver function cells and relative expression of the HNF1A function gene of the cells, and the method is simple and convenient to operate.

The application discloses a SSR molecular marker primer related to Chinese chives dormancy and a detection method and application thereof. In view of the problems that traditional Chinese chives dormancy trait identification relies on field morphological observation for 4 months, is inefficient and lacks special molecular markers, 7 specific SSR primers (SSR005 / 170 / 249 / 325 / 423 / 478 / 610) are developed based on cross-species genome resequencing (taking Chinese onion as a reference genome, and the alignment rate is 18.76%-18.89%) for the first time. Through the establishment of a matching detection system: CTAB method for rapid DNA extraction, optimization of the PCR system (20 muL contains 30 ng of template DNA, 0.4 U of Taq enzyme, 0.2 muM of primer, 0.2 mM of Mg 2+ 2.0 mM of dNTPs, 57 DEG C annealing), 8% polyacrylamide gel electrophoresis combined with silver staining development, the dormancy trait can be accurately identified within 7 days. For example, the SSR423 primer has no band at 177 bp, and the non-dormant / dormant germplasm cannot be distinguished. The detection results of 52 germplasms (such as dormancy germplasm 21-1 and non-dormancy germplasm 22-7) are consistent with the field phenotypes at a rate of 100%.

The invention provides a method for portable mass spectrum identification of bacterial species based on substrate-enzyme reaction, which comprises the following steps: culturing to-be-detected bacteria to obtain a bacterial culture solution, washing and diluting to obtain a bacterial suspension; adding a plurality of pre-selected substrates into the bacterial suspension for co-incubation, so that the bacterial suspension and the pre-selected substrates are subjected to enzyme reaction to obtain a bacterial substrate mixed solution; detecting the bacterial substrate mixed solution by using electrospray ionization mass spectrometry to obtain the mass spectrum signal intensity of the preselected substrates and the enzyme reaction product so as to calculate the conversion degree data of each preselected substrate; in a coordinate system based on a preselected substrate type and a transformation degree, drawing a characteristic column map of the to-be-detected bacteria; and matching the characteristic column spectrum with the characteristic column spectrum in the bacterium variety database, and identifying the variety of the to-be-detected bacterium. The method disclosed by the invention can realize species identification of bacteria in the bacterial infection biological sample within 30 minutes, has the advantages of rapidness, accuracy and wide application range, and can be suitable for field detection of the bacterial sample.

This utility model discloses a burr detection device for longitudinal shearing of silicon steel coils, belonging to the field of burr detection technology. It includes a trolley with a weighing and unwinding mechanism on its top, on which a longitudinally sheared silicon steel coil is mounted. A limiting mechanism is located on the side of the trolley, and a supporting mechanism is mounted on the limiting mechanism. A lifting box is fixedly connected to the top surface of the trolley, and a screw is rotatably connected to the inner cavity of the lifting box. A lifting seat is threaded onto the outer side of the screw. In this utility model, two stroboscopes capture images of the top and bottom of the released silicon steel sheet, and the captured images are transmitted to an external monitoring device. Under the action of the stroboscopes, the silicon steel sheet appears to be in a static state, thus allowing the monitoring device to clearly display defects. Even minute defects can be quickly identified, greatly improving production efficiency and reducing safety risks.