495results about How to "Rapid identification" patented technology

The invention discloses an Indel molecular marker for identifying a ricefield eel strain in the southwest region. The nucleotide sequences of the Indel molecular marker are respectively shown as SEQ ID NO: 9-12 or SEQ ID NO: 13-16. The invention also provides a primer pair for amplifying the Indel molecular marker. By adopting the Indel molecular marker primer pair provided by the invention to amplify genome DNA of ricefield eel strains in different regions, the ricefield eel strains in the southwest region can be quickly screened out according to amplified fragments. When the Indel marker provided by the invention is applied to practice, the genetic identification of the ricefield eel strain in the southwest region can be quickly realized, the domestication breeding of wild germplasm resources can be accelerated, and the precise breeding can be realized.

The invention discloses a method for identifying the authenticity of hybrid filial generations of tea trees based on SSR and KASP molecular markers, and belongs to the technical field of molecular biology and tea tree breeding. According to the invention, the authenticity of the hybrid offspring of two tea tree varieties is identified by utilizing a primer group designed by an SSR (Simple Sequence Repeat) marker, a method for reducing the number of tested samples is invented by combining the real offspring with re-sequencing result data of male and female parents, and an SNP (Single Nucleotide Polymorphism) molecular marker for identifying the hybrid offspring of the tea tree is developed. The KASP primer group capable of identifying the authenticity of the hybrid offspring of the tea tree is screened out, and by adopting the primer group disclosed by the invention, the identification of the authenticity of the hybrid offspring of the two tea trees can be realized, and the hybrid offspring polluted by pollen can be excluded; the identification method disclosed by the invention can replace an identification method for screening a specific SSR marker by utilizing a capillary electrophoresis technology, and the identification of the hybrid offspring of the tea tree can be quickly, accurately and effectively realized. Important basis and support are provided for molecular breeding for efficiently creating tea germplasm.

The invention belongs to the technical field of biomolecules, and particularly relates to an SMAD7 gene for judging chicken sex and application. Through the molecular marker and the design of a new PCR primer, the migration distance difference of two bands amplified by a ZW chromosome is 299bp, and compared with the migration distance difference of 150bp of a traditional sex identification gene CHD1 amplification system, the migration distance difference of the bands of the two items is larger, and judgment is easier; the fragments amplified by the designed PCR primer are smaller and are only 517bp and 816p, the PCR amplification time is remarkably shortened, and the sex identification time is shortened; in addition, amplification and agarose gel electrophoresis can be directly carried out by extracting chicken tissues, the sex of the chicken can be rapidly judged according to an electrophoresis result, the DNA extraction time is shortened, the time length can be controlled within 1 hour, operation is easy, the identification result is accurate, basic-level application and popularization are facilitated, and the method has a wide market application prospect.

The invention discloses a screening method of fentanyl substances, and belongs to the technical field of detection and identification of fentanyl substances. Comprising the following steps: S1, acquiring m types of fentanyl and fentanyl substance samples, and cracking to obtain characteristic warning fragments; s2, establishing a feature warning fragment database; s3, the warning ion database is imported into TraceFinder software, after a sample to be screened is split, fragments and characteristic warning fragments are deduced through the TraceFinder software according to the fragmentation law and the characteristic theory of each type of fentanyl, and whether the fragments and the characteristic warning fragments are fentanyl substances or not is judged. According to the method, n characteristic warning fragments of fentanyl are obtained after high-resolution mass spectrum pyrolysis characteristics of m known typical fentanyl compounds are obtained, and the characteristic warning fragments are used for constructing a database, so that fentanyl substances can be quickly screened and identified.

The invention discloses a gas phase fingerprint spectrum of a transverse meridian mat. The gas phase fingerprint spectrum comprises a peak 1-5, and the retention time of the peak 1-5 is 16.89 + / -0.1 min, 17.36 + / -0.1 min, 17.89 + / -0.1 min, 19.37 + / -0.1 min and 19.6 + / -0.1 min in sequence; the construction method of the gas-phase fingerprint of the membranaceous beautyberry root comprises the following steps: crushing membranaceous beautyberry root to obtain membranaceous beautyberry root powder, adding water into the membranaceous beautyberry root powder, adding ethyl acetate, slightly boiling for 4 hours, naturally cooling to room temperature, standing for layering, and collecting an ethyl acetate layer as a test solution; carrying out liquid chromatography detection on the test solution; the method comprises the following steps: collecting 10 or more membranaceous beautyleaf root medicinal materials, carrying out detection operation according to the method, comparing obtained detection results, and selecting common characteristic peaks to obtain the gas-phase fingerprint spectrum of membranaceous beautyleaf root. The gas-phase fingerprint spectrum constructed by the method can be used for rapidly and accurately determining and identifying the membranaceous beautyberry root traditional Chinese medicinal material.

The invention provides a primer for identifying tilapia mossambica as well as a detection kit and application thereof, and belongs to the technical field of molecular biology. The invention provides a primer for identifying the variety of tilapia mossambica, which can be used for quickly, efficiently and accurately identifying Oreochromis niloticus, Oreochromis aurea and Oreochromis spp., so that a scientific basis is provided for germplasm resource protection and variety identification of the tilapia mossambica.

The invention discloses a method for rapidly detecting bacterial drug resistance by combining a bacterial metabolism fingerprint spectrum after short-time antibiotic stimulation with machine learning. Bacteria are incubated in a liquid culture medium containing antibiotics with break point concentration for a short time, the metabolic pathway of the bacteria can be changed, and then generation of downstream metabolites is affected. As the drug-resistant bacteria have a special drug-resistant mechanism, the change of the metabolic state of the drug-resistant bacteria is different from that of the sensitive bacteria. Analyzing the metabolite fingerprint spectrum of the bacteria after short-time stimulation of the antibiotics by adopting matrix-assisted laser desorption ionization time-of-flight mass spectrometry; after data acquisition, classified analysis is performed through machine learning, so that rapid detection of the antibiotic resistance of bacteria is realized. Compared with the prior art, the method has the advantages that bacterial drug resistance detection can be completed within 2 hours, high practicability and operability are achieved, and meanwhile the method has the advantages of being high in throughput and low in experiment cost.

The invention discloses a database construction method for identifying cunninghamia lanceolata germplasm resources and application, and belongs to the technical field of biological breeding. The invention discloses a database construction method for identifying cunninghamia lanceolata germplasm resources. The method comprises the following steps: 1) collecting data; 2) data standardization processing; 3) designing a database; 4) creating a database; 5) algorithm integration; 6) establishing a database website; and 7) inputting, updating and maintaining data. The database for identifying the cedarwood germplasm resources comprises a cedarwood germplasm resource data management module, a picture management module, a data analysis module and a user management module. The result of the embodiment shows that the database is suitable for genetic diversity analysis, principal component analysis and comprehensive evaluation of the cunninghamia lanceolata germplasm resources.

The invention provides an SSR (Simple Sequence Repeat) marker primer group and a detection method for identifying filial generations of hibiscus varieties, and belongs to the technical field of molecular markers, and the SSR marker HBG18 is used for carrying out PCR (Polymerase Chain Reaction) detection on a male parent'hibiscus rosa 'and female parent'chiffon' hybrid combination and a male parent'blue bird 'and female parent'Han-plus' hybrid combination offspring; the SSR marker HBG7 is used for carrying out PCR detection on the hybridized combination of the male parent pink heart double petals and the female parent Chinese chiffon; the SSR marker HBG21 is used for carrying out PCR detection on a hybridized combination of a male parent'common powder 'and a female parent'red heart' and a hybridized combination of a male parent'blue chiffon 'and a female parent'long bract double petal', agarose gel electrophoresis detection shows that the three SSR markers can accurately distinguish hibiscus parents and hybrid filial generations of the hibiscus parents, and the purpose of rapidly identifying the hybrid F1 generation is achieved.

The invention discloses an Indel molecular marker for identifying ricefield eel strains in two Guangdong regions. The nucleotide sequence of the Indel molecular marker A is shown as SEQ ID NO: 5 or SEQ ID NO: 7; the nucleotide sequence of the Indel marker B is as shown in SEQ ID NO: 6 or SEQ ID NO: 8. The invention also provides a primer pair for amplifying the Indel molecular marker. By adopting the Indel molecular marker primer pair provided by the invention to amplify genome DNA of ricefield eel strains in different regions, the ricefield eel strains in the two regions can be quickly screened out according to amplified fragments. The Indel marker provided by the invention can be applied to rapid identification of germplasm resources of a specific geographical strain so as to obtain excellent characters of the strain and finally realize precise breeding.

The invention relates to the technical field of microbial identification, in particular to an identification method of a streptococcus thermophilus CICC 6063 strain and development and application of a strain level primer of the streptococcus thermophilus CICC 6063 strain. The invention provides an application of a nucleotide sequence as shown in SEQ ID NO: 1 in identification of a streptococcus thermophilus CICC 6063 strain. The invention provides an identification method of a streptococcus thermophilus CICC 6063 strain. The method comprises the following steps: extracting genome DNA of a to-be-detected strain, designing a primer aiming at a nucleotide sequence shown in SEQ ID NO: 1, amplifying the genome DNA, and judging whether the to-be-detected strain is the streptococcus thermophilus CICC 6063 strain or not by comparing the length of an actual amplification product with the length of a target amplification product and the condition of polymorphic site basic groups. The invention provides a simple and rapid method for identifying a streptococcus thermophilus CICC 6063 strain by utilizing a specific conserved gene sequence of streptococcus. The primer pair provided by the invention can be used for rapidly and accurately identifying the CICC 6063 strain on the strain level.

The invention discloses a canopy leaf vertical distribution index construction method, and relates to the field of crop monitoring, and the method comprises the steps: S1, manually measuring the plant height (PH) of each cell of corn, dividing the plant height into five layers according to the PH, and obtaining LAI values of the whole canopy and leaves of each layer; s2, fitting cumulative LAI values of different leaf layers by using a Logistic equation; s3, calculating a first-order derivative of the fitted curve to obtain a canopy LAD distribution curve; and S4, extracting the kurtosis (Kurtosis) index and the skewness (Skewness) index of the LAD curve. Meanwhile, a rapid and accurate estimation method for canopy configuration indexes is provided. The method has the advantages that a novel canopy leaf vertical distribution index is constructed according to accumulated LAI values of different heights; and fusing unmanned aerial vehicle multispectral, RGB and LIDAR data, estimating canopy LAI, plant height and constructed indexes, and realizing rapid identification of the corn plant type.

The invention provides a larimichthys crocea fast-growing character associated SNP marker combination and breeding application, the combination is prepared from a probe for detecting 10 SNP molecular marker combinations of larimichthys crocea fast-growing characters, and information of positions of all SNP molecular markers on chromosomes, alleles with changed sites and reference genes is shown in a table 1. According to the SNP marker combination disclosed by the invention, the optimal large yellow croaker individuals with fast-growing potential and a certain scale can be selected from the breeding offspring in advance for breeding and breed conservation, and the large yellow croaker individuals are bred to the parent without breeding and breed conservation of a large number of offspring seeds in the breeding process, so that the breeding cost can be greatly reduced. The SNP marker combination provided by the invention can solve the problem that non-lethal detection of the growth speed of the large yellow croaker cannot be realized in a traditional breeding mode, and can ensure smooth development of fast-growing character breeding work of the large yellow croaker.

The invention discloses a method and a system for rapidly identifying a photo-thermo-sensitive sterile line of forage grass sorghum, and relates to the technical field of agricultural remote sensing. The method comprises the following specific steps: taking a suspected photo-thermo-sensitive sterile line as a material to be detected; acquiring hyperspectral data of the to-be-detected material after heading and before pollen formation by using a hyperspectral imaging technology; extracting identification characteristics related to the photo-thermo-sensitive sterile line of the forage grass sorghum from the hyperspectral data; and establishing an identification model based on the identification characteristics, and inputting a to-be-detected sample into the identification model for rapid identification of the photo-thermo-sensitive sterile line. The method is based on a hyperspectral imaging technology and is combined with a machine learning algorithm to realize rapid, lossless, visual and accurate identification of the photo-thermo-sensitive sterile line of the forage grass sorghum, and the method can greatly improve the identification efficiency and accuracy.

The invention belongs to the technical field of biology, and relates to a barley germination period salt-tolerant gene HvHCT1, a protein and an application of the protein in salt damage stress resistance, and the protein comprises an amino acid sequence shown as SEQ ID NO: 2. According to the method, genetic loci related to the salt tolerance of the barley in the germination period are mined through a whole genome association analysis method, then transcriptome sequencing is used for revealing barley salt tolerance differential gene expression changes after salt damage stress, and in combination with association analysis and transcriptome sequencing data, salt tolerance candidate genes in the germination period of the barley are determined. The transgenic arabidopsis thaliana is used for verification; the research provides an excellent germplasm material and a new salt-tolerant gene for barley salt-tolerant breeding research, and also provides a theoretical basis for barley salt-tolerant molecular marker-assisted breeding in the future.

The invention discloses an InDel marker primer for identifying authenticity of sisal hemp interspecific hybridization F1 generation and application of the InDel marker primer. According to re-sequencing data of parent varieties of sisal hemp, an InDel marker primer for identifying the authenticity of a hybrid F1 generation of sisal hemp '292' and 'Induced 7' is developed, identification is carried out in a seedling stage of the hybrid F1 generation, and a result shows that offspring which can simultaneously amplify male parent and female parent characteristic bands is a true hybrid; the hybrid seeds are regarded as false hybrids when any one stripe is lacked or two stripes are lacked, so that the detection purity of the F1-generation real hybrid seeds is improved, and the identification time is advanced. Meanwhile, compared with a traditional hybrid identification method, the method for identifying the authenticity of the sisal hemp variety interspecific hybridization F1 hybrid is rapid, convenient, accurate, reliable, short in breeding period and low in breeding cost, breeding target offspring can be screened in advance, the identification efficiency of sisal hemp interspecific hybrids is effectively improved, and the method is suitable for popularization and application. And a more effective method is provided for rapidly identifying the authenticity of sisal hemp interspecific filial generations.

The invention discloses a KASP molecular marker closely linked with the early fruiting property of larch and application of the KASP molecular marker, and relates to the technical field of forest molecular marker-assisted breeding. The KASP molecular marker is characterized in that the basic group of G / A at the 1431418th site of a chromosome sca 1151: 1431418 is mutated; the nucleotide sequence of the KASP molecular marker is as shown in SEQ ID NO. 4. The KASP molecular marker primer disclosed by the invention can be used for rapidly identifying the premature fruit phenotype of the larch, has the advantages of accuracy, rapidness, low cost, short identification period, simplicity and convenience in operation and the like, can be used for assisting in new strain breeding of the larch, and has a wide application prospect.

The invention belongs to the technical field of plant breeding, and relates to a transgenic potato event ST2400643 and a detection method thereof. The invention provides a nucleic acid molecule for detecting a transgenic potato event ST2400643. The sequence of the nucleic acid molecule is shown as SEQ ID NO: 1-2 or a complementary sequence of the SEQ ID NO: 1-2. Or the sequence of the nucleic acid molecule is shown as SEQ ID NO: 3 or a complementary sequence thereof; the nucleic acid molecule is derived from a transgenic potato event ST2400643, and a potato plant containing the transgenic potato event ST2400643 has been preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC NO.46132. A potato plant comprising the transgenic potato event ST2400643 of the invention has the following advantages: the potato plant can tolerate imidazolinone herbicides; the potato yield is not reduced; and the breeding efficiency is enhanced, and the molecular marker can be used for tracking transgenic insertion fragments in a breeding population and a progeny thereof.

The invention discloses a construction method and application of an HPLC (High Performance Liquid Chromatography) specific chromatogram of sunburn prevention and treatment cream, and relates to the technical field of pharmaceutical analysis. The sunshine prevention and treatment cream is composed of honeysuckle, perfoliote knotweed herb, sedum sarmentosum, dayflower, radix polygonati officinalis, lithospermum erythrorhizon, aloe, asparagus fern, lucid ganoderma, coix seed and bee pollen, and is one of representative products of Guizhou Miao medicine brands. The specific chromatogram of the sunshine prevention and treatment cream is established by adopting a high performance liquid chromatography, and the five medicinal materials including honeysuckle, lithospermum erythrorhizon, aloe, sedum sarmentosum and perfoliote knotweed herb can be identified at the same time. The HPLC characteristic chromatogram of the sunshine prevention and treatment cream established by the method can effectively characterize the quality of the sunshine prevention and treatment cream, and is beneficial to comprehensively monitoring the quality of medicinal materials. Meanwhile, the method has the advantages of being simple, convenient, stable, high in precision, good in reproducibility and the like, and the authenticity of the product can be rapidly and accurately identified.

The invention discloses a near-infrared fluorescence sensor, a near-infrared fluorescence sensor array and a preparation method and application of the near-infrared fluorescence sensor array, and belongs to the technical field of biological medicine. The composite material comprises a single-walled carbon nanotube, a single-stranded DNA (deoxyribonucleic acid) and hydrogel, the single-stranded DNA is connected and modified on the surface of the single-walled carbon nanotube, and the single-walled carbon nanotube modified with the single-stranded DNA is fixed in the hydrogel. Bacterial metabolites enter the hydrogel and influence the structure of the single-stranded DNA, so that the fluorescence intensity of the single-walled carbon nanotube is influenced. A plurality of near-infrared fluorescence sensors form an array to quickly capture component change caused by growth of bacteria in an object to be detected, and respond to fluorescence signal change, so as to visualize whether the components change or not. Whether bacteria grow or not is judged through response information of the multiple sensors, so that the bacteria are rapidly recognized, and the method has the advantages of being high in sensitivity, short in response time and small in needed sample size.

The invention relates to the technical field of edible mushrooms, in particular to a molecular marker for detecting the pileus color of a selfing population of flammulina velutipes and application of the molecular marker. The molecular marker for detecting the pileus color of the needle mushroom selfing population can rapidly and accurately identify the pileus color of the brown needle mushroom selfing population, has the advantages of being accurate, efficient, good in typing effect and the like, can accelerate screening of target varieties, is used for assisting breeding of needle mushroom varieties, provides technical support for needle mushroom breeding work, and has wide application prospects. A theoretical basis is laid.

The invention belongs to the technical field of molecular biology, and particularly relates to a pseudo-ginseng component rapid detection method based on a Proofman-LMTIA technology. According to the method, a pseudo-ginseng ITS2 sequence is taken as a specific target, and high-specificity and high-sensitivity detection of pseudo-ginseng components is realized through a designed LMTIA primer and a Proofman probe. Compared with the traditional detection technology, the invention has the advantages of simplified primer design, low-cost equipment requirement, rapid detection (only about 30 minutes) and wide application range. The method is not only suitable for detection of raw materials such as pseudo-ginseng seeds, seedlings, roots and rhizomes, decoction pieces and the like, but also can be used for authenticity identification of terminal products such as food, medicines, health care products and the like containing pseudo-ginseng components. Through innovative application of the Proofman-LMTIA technology, an effective solution is provided for rapid and accurate identification of pseudo-ginseng and derivative products thereof, and the method has important market application prospects and social benefits.

The invention belongs to the field of molecular genetic breeding, and particularly relates to a KASP molecular marker method related to peanut aspergillus flavus seed kernel infection resistance and application of the KASP molecular marker method. Through whole genome association analysis, the SNP locus is located at the 133198521th bp position of the peanut B06 chromosome (the reference genome is peanut), the nucleotide sequence polymorphism is C / A (the antisense strand is G / T), and the SNP locus is named as qARIB06. Based on the SNP site, KASP molecular marker primer sequences (SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3) are designed and developed, and the KASP molecular marker primer sequences are used for rapidly and accurately identifying the genotype of a peanut sample.

The invention discloses a lithium battery combined health state estimation and service life prediction method, which comprises the following steps of: 1, predicting the health state and the residual service life of a lithium battery by carrying out pulse test on the lithium battery and establishing a Thevenin model at the aging normal operation stage of the lithium battery; 2, after the lithium battery enters a deep aging stage, performing an EIS test on the lithium battery to obtain impedance spectrum data, and correcting the Thevenin model into a variable order RC model; and 3, estimating the health state of the deep aging stage by using the impedance spectrum data and parameters identified by the variable order RC model, and when the prediction error of the two parameters is smaller than an error threshold value, considering that a battery health state and residual life prediction model suitable for the deep aging stage is successfully established. Otherwise, adjusting the order of the variable-order RC model to reduce the error. According to the invention, the reliability of lithium battery life prediction and estimation in a complete life cycle can be ensured, so that the control complexity and cost of a test working condition can be effectively reduced.

The invention belongs to the technical field of food safety detection, and discloses a dual-mode aquatic product freshness intelligent indication label and a preparation method and application thereof. The preparation method comprises the steps that after gold nanoparticles are dispersed in a reducing agent aqueous solution, a silver salt solution is dropwise added, then vibration treatment is conducted, and gold-core silver-shell nanoparticles are obtained; adjusting the pH value of the anthocyanin concentrated solution to be acidic, and uniformly mixing the anthocyanin concentrated solution with the gold-core silver-shell nanoparticles to obtain a gold-core silver-shell nanoparticle / anthocyanin aggregate solution; and dispersing agar and polyvinyl alcohol in water, heating to form gel, adding the gold-core silver-shell nanoparticle / anthocyanin aggregate solution at 45-50 DEG C, uniformly mixing, and cooling and solidifying to obtain the dual-mode intelligent indication label for freshness of aquatic products. The preparation method provided by the invention is simple and easy to operate, green and environment-friendly, and easy for batch preparation, and the prepared dual-mode aquatic product freshness intelligent indication label can realize sensitive and accurate quantitative analysis of aquatic product freshness indexes.

The invention discloses a chicken embryo sex determination kit directly using blood as a template and application thereof. Belongs to the technical field of biology. The kit comprises Taq DNA polymerase, an upstream primer, a downstream primer and water. Compared with the prior art, the method has the beneficial effects that a PCR reaction system is optimized by adopting the super-fidelity Taq DNA polymerase, whole blood is diluted and then is directly used as a template for PCR reaction, tedious DNA extraction is not needed, meanwhile, a PCR reaction enhancer and a PCR system stabilizer are not needed to be added into the reaction system, and the operation is simple and convenient. The method has the advantages of simple and convenient operation, less time consumption, rapid identification and the like, and avoids the defects of time and labor consumption and error proneness of a traditional method.

The invention relates to the technical field of traditional Chinese medicine detection, and particularly provides a construction method of specific chromatograms of common carpesium fruit and a preparation thereof. Comprising the following steps: by taking octadecylsilane chemically bonded silica as a filling agent and a mobile phase comprising methanol and an acid-containing aqueous solution, carrying out gradient elution, and screening through a large number of experiments to obtain a specific elution procedure, so as to obtain 13 or more common characteristic peaks, and realizing the purification of the chlorogenic acid, the caffeic acid, the chlorogenic acid, the cryptochlorogenic acid, the 1, 4-dicaffeoylquinic acid, the 1, 4-dicaffeoylquinic acid, the 1, 4-dicaffeoylquinic acid, the 1, 4-dicaffeoylquinic acid and the 1, 4-dicaffeoylquinic acid. The method can effectively separate characteristic peaks of 2, 5-dicaffeoylquinic acid, cosmosiin and carabrone, is good in peak shape, stable in base line, short in detection time, provides a basis for quality detection and control of the common carpesium fruit and the preparation thereof, can fully reflect the integrity and characteristics of the common carpesium fruit and the preparation thereof, is simple to operate, and is suitable for industrial production. The method is suitable for quality control of different preparation types of common carpesium fruit.

The invention discloses a rapid sex identification method for propylaea japonica adults, and relates to the technical field of insect sex identification. Collecting adult propylaea japonica; if triangular black spots or hourglass-shaped black spots exist in the middle of the forehead of the head of the adult, the adult is female; if the middle part of the forehead of the head of the adult has no triangular black spot and is yellow or milky copper bell-shaped, the adult is male; the propylaea japonica adults are adults within the third day to the thirtieth day of eclosion, the elytra color spots are in a turtle pattern shape, and the forehead of the head is yellow or milky white. According to the method disclosed by the invention, the cost is hardly needed, the operation is very simple, the sex of the propylaea japonica adults can be accurately identified under the condition of hardly damaging the propylaea japonica adults, and related researchers can completely grasp the sex of the propylaea japonica adults only by being slightly familiar with the forehead morphological characteristics of the adults.

The invention provides an industrial robot kinetic parameter identification method based on an adaptive mutation particle swarm algorithm, and the method comprises the following steps: S1, building a kinetic model according to a DH parameter of a robot and a Newton-Euler kinetic equation, and directly determining a minimum parameter set according to the DH parameter and a joint type; s2, optimizing two mixed trajectories of Fourier series and polynomial in MATLAB (matrix laboratory); s3, driving the robot to run an excitation track, collecting data, and performing filtering calculation to obtain an observation matrix and a joint torque; s4, calculating a group of initial solutions through a least square method, and taking the initial solutions as a particle swarm optimization algorithm search range of adaptive variation in a certain range of the initial solutions; and S5, driving the robot to run the verification track, and processing the collected data to obtain an observation matrix of the verification track. According to the method, the mixed trajectory based on the Fourier series and the polynomial serves as the excitation trajectory, one-time overall identification is carried out through the self-adaptive variation particle swarm optimization algorithm, the identification result is accurate, and the predicted torque following performance is high.

The invention belongs to the field of biological detection, and particularly relates to a kit and a method capable of doubly detecting verticillium dahliae and fusarium oxysporum. The optimal primer pair aiming at verticillium dahliae and fusarium oxysporum is found, an RPA single detection method and an RPA dual detection method are established based on the primer pair, and the RPA-LFD primer pair is suitable for RPA-LFD detection. Compared with the common technology for detecting the verticillium wilt at present, the method has the advantages that high specificity and low detection limit can be ensured, verticillium dahliae and fusarium oxysporum in related cotton samples can be detected more sensitively, quickly and conveniently, dependence on professional technicians and laboratory precise instruments can be avoided, and the method is suitable for large-scale popularization and application. The method is of great significance to rapid and accurate identification, monitoring and early prevention and treatment of cotton verticillium wilt and fusarium wilt.