33 results about "Cunninghamia" patented technology

The invention discloses a database construction method for identifying cunninghamia lanceolata germplasm resources and application, and belongs to the technical field of biological breeding. The invention discloses a database construction method for identifying cunninghamia lanceolata germplasm resources. The method comprises the following steps: 1) collecting data; 2) data standardization processing; 3) designing a database; 4) creating a database; 5) algorithm integration; 6) establishing a database website; and 7) inputting, updating and maintaining data. The database for identifying the cedarwood germplasm resources comprises a cedarwood germplasm resource data management module, a picture management module, a data analysis module and a user management module. The result of the embodiment shows that the database is suitable for genetic diversity analysis, principal component analysis and comprehensive evaluation of the cunninghamia lanceolata germplasm resources.

The invention provides a cunninghamia lanceolata tree height curve prediction method based on a cunninghamia lanceolata phoebe bournei near-natural transformation forest stand, and the method comprises the steps: completing the construction of a cunninghamia lanceolata tree height curve model in a cunninghamia lanceolata phoebe bournei near-natural transformation mode by using a multivariate and dumb variable combined and a basic method support is provided for estimating the height of the cedarwood in the near-natural transformation mode of the cedarwood phoebe bournei, subsequently estimating the cedarwood storage amount and the wood outturn amount of the wood species, and screening a proper near-natural transformation mode of the cedarwood phoebe bournei.

The invention relates to the technical field of construction of clone cutting orchards of China fir, in particular to a method for constructing a cutting orchard by grafting and cultivating stock plants through root stocks of China fir. According to the method, the cunninghamia lanceolata roots serve as stocks, and branches on excellent single plants are grafted. The stock plant survived after grafting does not germinate coppice shoots due to the fact that the rootstock is a seedling root. After earthing, the grown coppice shoots are coppice shoots on the scions, namely the coppice shoots of excellent clonal materials. Moreover, after the soil covering treatment, the coppice shoots growing from the soil are subjected to a'yellowing process', so that the'rejuvenation 'effect of the coppice shoots is realized. When the coppice shoots are used for cutting seedling raising, the effect is good, growth is rapid, and the problem that the survival rate is low when branches are picked from forest branches for cutting is effectively solved. The method can be used for building a cutting orchard in the current year, and is an effective method for rapidly breeding clone scions with large yield increasing benefits. The application can bring remarkable economic benefits to forestry production.

The invention discloses bacillus subtilis Bs1 as well as an antagonistic substance and application thereof. According to the invention, Bs1 with antagonism on colletotrichum gloeosporioides is separated and screened for the first time, an antagonistic substance is clear as Bs1 serine-producing protease Vpr, a Bs1 bacteriostasis mechanism and biological control potential excavation are analyzed in a multi-omics manner, a peripheral colonization rule of Bs1-Rif is clear, and an indoor control strategy of the strain Bs1 on cunninghamia lanceolata anthracnose is optimized. When the strain Bs1 is applied on the day when pathogenic bacteria are inoculated, a certain prevention and treatment effect is achieved, and it is indicated that the strain Bs1 can rapidly occupy the ecological niche; the average antibacterial rate of the strain Bs1 can reach 66.69%. The biocontrol strain and theoretical basis are provided for prevention and control of Chinese fir diseases, the application of biological prevention and control in forestry is promoted, the interaction mechanism of biocontrol bacteria and pathogenic bacteria can be deeply understood, and the sustainable development requirement of modern forestry is met.

The utility model discloses efficient cedarwood chip drying equipment, and belongs to the field of cedarwood drying, the efficient cedarwood chip drying equipment comprises a box body, a rotating device is arranged on the box body, the rotating device comprises a fixing plate, the bottom of the fixing plate is rotatably connected with a first rotating rod through a bearing, and the first rotating rod movably penetrates through the fixing plate and extends to the bottom; and the top of the first rotating rod is fixedly connected with a second rotating rod. The cunninghamia lanceolata is placed on the top of the tray, the heating pipe is started to dry the cunninghamia lanceolata, the output end of the motor drives the tray to rotate, each cunninghamia lanceolata can be heated to be dried, and due to the fact that all the cunninghamia lanceolata can be evenly heated, the efficiency of the whole drying process Therefore, the drying task can be completed more quickly, the production efficiency and the product turnover rate are improved, and the problem that different positions of the cunninghamia lanceolata cannot be dried when the cunninghamia lanceolata is dried in the prior art is solved.

The invention discloses a method, a device, equipment, a medium and a product for estimating the outturn quantity and the outturn rate of non-saving materials of a Chinese fir based on a cutting degree equation, and relates to the technical field of forest cultivation, the method comprises the following steps: estimating the height and the diameter of a Chinese fir trunk based on non-saving material data of the Chinese fir according to an optimal cutting degree equation model of the Chinese fir; determining the pruning height and the pruning strength of the cedarwood; the China fir non-section material comprises a China fir non-section plate and a China fir non-section column material. Based on the pruning strength and the pruning height of the cedarwood, the non-saving wood outturn amount of the cedarwood is determined; according to the method, the non-saving material outturn percentage of the cunninghamia lanceolata is determined based on the non-saving material outturn percentage of the cunninghamia lanceolata, so that the problems that only the outturn percentage and the outturn percentage of normal forests can be estimated, limitation is large, and especially for defective forests, the non-saving material outturn percentage of the cunninghamia lanceolata and the outturn percentage evaluation error is large are solved.

The utility model relates to a cunninghamia lanceolata florescence pollination cover which comprises a C-shaped ring, a clamping assembly is arranged in the C-shaped ring, a conical cover is fixedly installed at the bottom of the C-shaped ring, a cylinder is fixedly installed at the bottom of the conical cover, notches corresponding to the C-shaped ring are formed in the side wall of the conical cover and the side wall of the cylinder, and the C-shaped ring is arranged in the C-shaped ring. Sliding grooves are formed in the outer wall of the C-shaped ring and the outer wall of the cylinder, sliding blocks are installed in the two sliding grooves in a sliding mode, and plastic outer covers are fixedly installed on the outer sides of the two sliding blocks. According to the pollination sleeve cover for the flowering phase of the cunninghamia lanceolata plant, firstly, a cunninghamia lanceolata branch with a ball flower is put into the device through the notch, then the plastic outer cover is slid to cover the notch, pollen spraying equipment can be used for pollinating the cunninghamia lanceolata autumn flower at the bottoms of the cylinder and the plastic outer cover after covering, and after pollination is completed, the plastic outer cover is slid to the outside of the device and can be rapidly taken down. The whole use process is simple and convenient to operate, and the pollination efficiency is remarkably improved.

The application discloses a method for regulating growth morphological indexes of Cunninghamia lanceolata seedlings and belongs to the technical field of plant growth promotion. ‑2 ·s ‑1 With 40 μmol m ‑2 ·s ‑1 mol m Blue light, red light, 1:1 L red / far red light, 1:2 L red / far red light and far red light with 40 μmol m The results show that the root system of the Cunninghamia lanceolata seedlings cultivated under 68% light intensity grows more vigorously, the increment of lateral branch length of the Cunninghamia lanceolata seedlings increases under low light intensity, the root-shoot ratio decreases, and the leaf area is large and thin.Compared with white light, blue light can promote the elongation of the Cunninghamia lanceolata seedling height, but the seedling stem thickening is inhibited, and the seedling root system grows weakly with low root-shoot ratio.Red light promotes the growth of the Cunninghamia lanceolata seedling leaves and root system, and the root-shoot ratio is high, but the elongation of the Cunninghamia lanceolata seedling height is inhibited.The far red light and the increase of the far red light proportion are beneficial to the elongation of the Cunninghamia lanceolata seedling height.

The invention discloses a construction method of a cunninghamia lanceolata genetic transformation system, and belongs to the technical field of genetic transformation. The invention discloses a construction method of a cunninghamia lanceolata genetic transformation system, which comprises the following steps: preparing an EHA105 agrobacterium infection solution with OD600 of 1.2 by taking a 11C genotype cunninghamia lanceolata tissue culture seedling as a transformation receptor, carrying out-60kPa vacuumizing treatment for 25 minutes, and co-culturing in a dark environment at 23 DEG C for 48 hours after infection to complete transformation. According to the method, an efficient instantaneous genetic transformation system which is simple and convenient to operate is successfully established, the problem that traditional breeding genetic gain of the cunninghamia lanceolata is limited is solved, key parameters such as bacterial liquid concentration, infection time and co-culture conditions are optimized through an orthogonal test by utilizing an agrobacterium tumefaciens-mediated instantaneous transformation technology, and the transformation efficiency is remarkably improved. GUS enzyme activity determination and dyeing verification show that the system is high in transformation efficiency and good in repeatability, and an efficient technical platform is provided for Chinese fir gene function research and genetic improvement.

The invention discloses a planting method for improving the soil quality of a Chinese fir forest by cultivating ganoderma lucidum in bags. The planting method comprises the following steps: selecting a Chinese fir forest land planted for more than 5 years; adding water into sawdust, wheat bran and lime according to a mass ratio of 180: 40: 3, uniformly mixing, filling into a plastic barrel bag to prepare a mushroom stick, and sterilizing; inoculating the mushroom sticks with ganoderma lucidum strains in a sterile environment by adopting a two-end inoculation mode; carrying the inoculated mushroom sticks into a culture field, and carrying out crisscross stacking culture for 30-40 days; ditching between lines of the cunninghamia lanceolata, removing the plastic tube bags from the cultured ganoderma lucidum sticks, putting the ganoderma lucidum sticks into the ditches, and covering soil; and a step of ganoderma lucidum emergence management. The fungus stick substrate takes agricultural and forestry organic wastes (such as wood chips and bran) which can be quickly degraded as main components, and can be continuously mineralized and decomposed in the growth process of ganoderma lucidum mycelia, so that a large amount of water-soluble organic carbon, nitrogen and other mineral nutrients can be released to soil in a short time, and the fertility of surface soil is remarkably improved.

The invention discloses a breeding method for fir stem tip tissue culture, and belongs to the technical field of plant tissue culture. Comprising the following steps: (1) selecting a stem tip on an excellent seed tree as an explant, sterilizing, inoculating to a callus induction culture medium, and culturing and inducing to obtain a callus; then inoculating to a callus proliferation culture medium to carry out callus proliferation; (2) carrying out hot air treatment on the proliferated callus; and (3) sequentially inoculating the callus treated by the hot air to an adventitious bud induction culture medium and a rooting culture medium to form tissue culture seedlings. According to the breeding method for cedarwood stem tip tissue culture, the culture period is remarkably shortened, operation is easy, cost is low, the method is not limited by seasons, and the survival rate is greatly increased.

The invention discloses burkholderia with phosphorus dissolving and plant growth promoting functions and application of the burkholderia. The invention provides burkholderia cunninghamiae or a progeny of the burkholderia cunninghamiae, the burkholderia cunninghamiae is burkholderia cunninghamiae, the strain number of the burkholderia cunninghamiae is SSBN10R, and the registration number of the burkholderia cunninghamiae in the China General Microbiological Culture Collection Center is CGMCC (China General Microbiological Culture Collection Center) No. 35175. The cunninghamia lanceolata Burkholderia sp. Provided by the invention has the characteristics of dissolving phosphorus and producing IAA, and can promote the growth of cunninghamia lanceolata and increase the content of available phosphorus in soil. The method has important significance in the aspects of Chinese fir cultivation, soil improvement, microbial resource research, biological fertilizer development and the like.

The invention discloses a method for screening soil under a cunninghamia lanceolata forest by carbon sequestration bacteria and application of the method. The carbon sequestration bacteria provided by the invention are Gemmobacter lutimaris bacteria, the carbon sequestration bacteria are separated and screened by adopting a mode of combining three culture mediums, namely a carbon-free inorganic liquid culture medium, a carbon-free inorganic solid culture medium and an LB solid culture medium, and compared with other bacteria screening technologies singly using the carbon-free inorganic culture medium, the method has the advantages of low cost, high efficiency, convenience in operation and wide application prospect. The strain is screened from the soil under the cunninghamia lanceolata forest in the karst region of Guizhou province, the strain has high carbon sequestration capacity, carbon can be effectively stored in the soil, and soil carbon adsorption and storage and accumulated carbon sequestration are facilitated.

The invention discloses a cunninghamia lanceolata seedling growth state recognition and quantity statistics method and device based on an improved YOLOv5s network, a medium and a product, and relates to the technical field of target detection, and the method comprises the steps: obtaining a target image; the target image is an image of a Chinese fir seedling planting area to be detected; inputting the target image into a cunninghamia lanceolata seedling detection model to obtain the target image marked with the prediction frame and a prediction value of the growth state of each cunninghamia lanceolata seedling in the cunninghamia lanceolata seedling planting area to be detected; the cunninghamia lanceolata seedling detection model is obtained by training an improved YOLOv5s network, and the improved YOLOv5s network is constructed based on a ShuffleNetV2 model, an ESCA module and the YOLOv5s network; and based on the target image marked with the prediction box, determining the number of the cedarwood seedlings in the cedarwood seedling planting area to be detected. The efficiency and precision of Chinese fir seedling growth state recognition and quantity statistics are improved.

The invention provides a large-range cedarwood stock estimation method and system based on a CR-RF hybrid model, and the method comprises the steps: 1, collecting Landsat time series data in a research region, and carrying out the data preprocessing; step 2, constructing NBR annual median synthesis time sequence data; 3, post-processing the forest age data to obtain a Chinese fir forest age distribution diagram in the research area; 4, taking the forest age of the cunninghamia lanceolata obtained in the step 3 in combination with the auxiliary data as an independent variable, and taking the actually measured stock of the sample plot as a dependent variable to construct a specific growth equation of the region; 5, extracting growth process variables from the Landsat long time series data, and performing variable screening by adopting a recursive feature elimination algorithm RFECV based on cross validation; step 6, constructing a hybrid model CR-RF by using a random forest RF method; and step 7, exporting a spatial distribution diagram of the cedarwood stock through the hybrid model CR-RF trained in the step 6. By applying the technical scheme, the robustness and interpretability of the accumulation estimation under different ecological regions and operation intensities can be improved.

According to the preparation technology of the carbon dot modified flame-retardant cunninghamia lanceolata plywood and the plywood, a cunninghamia lanceolata veneer is combined with flame-retardant carbon dot impregnation liquid, flame-retardant carbon dots are made to evenly permeate into wood of the cunninghamia lanceolata veneer through vacuum impregnation, and the flame-retardant performance of the cunninghamia lanceolata veneer is effectively improved; meanwhile, the natural mechanical property of the cedarwood veneer is kept. In the process, the impregnation pressure and the hot pressing parameters are accurately controlled, so that tight combination of the flame-retardant carbon dots and the cedarwood veneers is ensured, and the manufactured plywood has excellent stability and mechanical strength. By optimizing the drying temperature and the water content of the impregnated veneers, the problem of wood cracking caused by traditional flame retardant treatment is effectively avoided. The flame-retardant adhesive is suitable for the field of building decoration with high requirements on fireproof performance and environmental protection, and has a remarkable market application value.

The invention discloses a cunninghamia lanceolata chloroplast SNP molecular marker and application thereof. The invention develops a cunninghamia lanceolata chloroplast SNP molecular marker combination for individual identification of cunninghamia lanceolata, the cunninghamia lanceolata chloroplast SNP molecular marker combination comprises 60 cunninghamia lanceolata chloroplast SNP loci, and after a cunninghamia lanceolata variety to be detected is amplified by using a primer pair capable of specifically amplifying the chloroplast SNP molecular marker combination, sequencing is performed to obtain an SNP sequence of the characteristics of the Therefore, through the SNP molecular marker and the amplification primer thereof developed by the invention, the cedarwood sample can be accurately and efficiently identified, and technical support is provided for protection and utilization of high-quality cedarwood individual resources.

The present application belongs to the technical field of species provenance tracing, and particularly relates to a primer set of molecular markers for identifying populations of endangered plant Cunninghamia lanceolata and application thereof. The present application provides five molecular markers DYS, SHS, BMS, HP and DLS for identifying populations of endangered plant Cunninghamia lanceolata for the first time, wherein the molecular markers DYS, SHS, BMS, HP and DLS are respectively used for identifying the Dayaoshan population, the Shunhuangshan population, the Bamianshan population, the Huaping population and the Daluoshan population of Cunninghamia lanceolata. Then, a primer set for amplifying the molecular markers DYS, SHS, BMS, HP and DLS is designed, and a tracing method of the source of Cunninghamia lanceolata is developed. The method can trace the geographical source of Cunninghamia lanceolata simply and efficiently, and provides reliable technical support for the judicial identification and species control of the endangered plant Cunninghamia lanceolata.

The invention provides a mountain fir large-diameter timber ecological forest culture method, and belongs to the technical field of forestry. The invention relates to a mountain fir large-diameter timber ecological forest culture method which is used for transforming a fir degenerated man-made forest or a fir pure man-made forest. The method comprises the following steps of cutting and pile killing, ecological weed control, forest land design, deep plowing and pier making, fir making in a forest belt, weed control in the forest belt, pier protection and fertilization, ecological maintenance and large-diameter timber separated cutting and culture. According to the mountain China fir large-diameter timber ecological forest culture method, ecological prior ecological forest culture new-quality productivity is implemented, about 2 / 3 of the forest land is not cultivated to keep original ecology, the biological diversity of the forest land is protected, and about 1 / 3 of the forest land is used for building a forestation zone for culturing China fir large-diameter timber. The method can promote fast growth of the cunninghamia lanceolata, achieves the standard of large-diameter materials, and gives consideration to efficient and sustainable operation of the forest land.

The invention discloses an interplanting mode and method of a new Chinese fir variety and camellia oleifera, and belongs to the technical field of artificial afforestation. According to the method, a multi-layer cultivation and composite operation mode which can be in symbiotic complementation with oil-tea camellia is built by utilizing the variation characteristics of narrow and small crown and high and straight trunk of a new Chinese fir variety, namely, Hubei cunning-1 (cunninghamia lanceolata (cunninghamia lanceolata for short); the selected tree species are scientific and reasonable in structure, the wide crown breadth of the camellia oleifera and the narrow crown breadth of the cunninghamia lanceolata are mutually spaced, the high-trunk type of the cunninghamia lanceolata and the low-trunk type of the camellia oleifera are spatially staggered, the camellia oleifera can be shaded in the early stage, the crown layering in the later stage can keep sufficient illumination in the forest, and the two tree species form a symbiotic and complementary spatial structure relationship; meanwhile, the forest stand structure is more optimized, the young forest canopy density is improved, the bare forest land is reduced, the forest land environment is improved, the forest stand quality, the health condition, the operation benefits and the ecological landscape effect are effectively improved, one-land multi-purpose and one-land multi-harvest are achieved, efficient utilization of land resources is promoted, and the ecological, economic and social multifunctional benefits of artificial forests are brought into play; diversified and high-quality supply of forest products such as oil and wood is guaranteed, and the double purposes of sustainable operation of artificial forest land and high-quality development of the camellia oleifera industry are achieved.

The invention provides a forest waste biochar base fertilizer for coastal low-lying land soil improvement, which is characterized in that biochar with different characteristics is prepared through a directional carbonization process by utilizing the difference of tree wastes such as leaves, barks and branches in fiber density and chemical composition, and pseudomonas putida and montmorillonite are respectively loaded according to the porosity difference. Layered matching is carried out according to requirements of soil layers, the physical structure of soil is effectively improved, hardening and salt stress are relieved, meanwhile, the long-acting slow-release characteristic is achieved, the nutritional requirements of taxodium orientalis seedlings in the whole growing season can be continuously met, overground part growth and root development of taxodium orientalis can be effectively promoted, and the survival rate of taxodium orientalis seedlings is increased. And a green, economic and sustainable technical approach is provided for ecological restoration and vegetation reconstruction of the coastal saline-alkali land.

The invention discloses a cunninghamia lanceolata seed source breeding and configuration method integrating a hydraulic structure and resource utilization efficiency, and belongs to the technical field of seed source breeding. The method comprises the following steps: S1, establishing a cunninghamia lanceolata provenance functional trait database based on leaf traits, wood traits, root traits and hydraulic traits of cunninghamia lanceolata of different And S2, constructing a water-carbon efficiency strategy map based on the provenance functional character database, and carrying out Chinese fir breeding and configuration on different regions based on the water-carbon efficiency strategy map. According to the method, the water consumption-carbon sequestration tradeoff relation of different provenances is quantified, and a quantitative basis is provided for regional water resource management and carbon sink afforestation.

This invention discloses a method for the selection and configuration of Chinese fir (Cunninghamia lanceolata) germplasm that integrates hydraulic structure and resource utilization efficiency, belonging to the field of germplasm selection technology. The method includes: S1, establishing a functional trait database of Chinese fir germplasm based on leaf, wood, root, and hydraulic characteristics of different germplasm sources; S2, constructing a water-carbon efficiency strategy map based on the germplasm functional trait database, and selecting and configuring Chinese fir in different regions based on the water-carbon efficiency strategy map. This invention quantifies the "water consumption-carbon sequestration" trade-off relationship of different germplasm sources, providing a quantitative basis for regional water resource management and carbon sequestration afforestation.

The invention provides a Chinese fir pruning height snap ring and a using method, the Chinese fir pruning height snap ring comprises an up-and-down walking assembly and a diameter measuring assembly, the up-and-down walking assembly comprises a surrounding frame and four roller telescopic assemblies, the surrounding frame comprises a surrounding frame body and a movable frame plate, the surrounding frame body and the movable frame plate are detachably connected, and the four roller telescopic assemblies are arranged on the surrounding frame body. The surrounding frame body and the movable frame plate are assembled to form a closed shape; the four rolling wheel telescopic assemblies are distributed on two crossed straight lines, each rolling wheel telescopic assembly comprises a rolling wheel and a spring, and the rolling wheels are arranged on the surrounding frame in a telescopic sliding mode through the springs; the diameter measuring assembly comprises two detection plates and a distance measuring sensor, the two detection plates are fixed to the two roller telescopic assemblies located on the same cross straight line, and the transmitting end and the receiving end of the distance measuring sensor are fixed to the two detection plates respectively. The Chinese fir trunk diameter can be measured in real time.

The invention discloses a cunninghamia lanceolata forest soil plant silicon body carbon sink potential estimation method, and relates to the field of data analysis, and the method comprises the steps: selecting a sampling land based on a cunninghamia lanceolata forest distribution region, a site condition type and stand development integrity, and dividing the sampling land into an initial stage forest, a middle stage forest, a mature stage forest and a special regeneration forest according to stand development stages, selecting grids in the sample plot based on three-dimensional grid division and random sampling so as to collect soil samples of soil layers with different depths; through the design of sample plot selection and three-dimensional grid sampling, the representativeness and uniformity of soil sample collection are improved, the accuracy of detection indexes is ensured through standard pretreatment and an efficient plant silicon body extraction technology, meanwhile, core driving factors are analyzed and identified in combination with multi-dimensional coupling, and the accuracy of the detection indexes is improved. Forest stand development and environmental factors are integrated into the constructed dynamic correlation model, and the carbon sink scale of each stage can be accurately deduced.

The application discloses a Chinese fir chloroplast SNP molecular marker and application thereof. The application develops a Chinese fir chloroplast SNP molecular marker combination for identifying Chinese fir individuals, and the combination comprises 60 Chinese fir chloroplast SNP sites. After primers capable of specifically amplifying the chloroplast SNP molecular marker combination are used to amplify a Chinese fir variety to be tested, sequencing is performed, and a SNP sequence characteristic of the Chinese fir variety to be tested is obtained. Therefore, by using the SNP molecular marker and the amplification primer thereof developed by the application, Chinese fir samples can be accurately and efficiently identified, and technical support is provided for high-quality Chinese fir individual resource protection and utilization.

The invention discloses an efficient construction method of a cunninghamia lanceolata genetic transformation system with seeds as materials, and belongs to the technical field of genetic transformation. The efficient construction method of the cunninghamia lanceolata genetic transformation system taking the seeds as the materials mainly comprises the following steps: disinfecting and peeling the cunninghamia lanceolata seeds, sequentially performing germination culture, adding the cunninghamia lanceolata seeds into an agrobacterium suspension, performing vacuum-pumping infection treatment, performing co-culture, performing degerming culture to form seedlings, taking stem segments of the seedlings as explants, and culturing the explants to obtain the cunninghamia lanceolata genetic transformation system. And inoculating to an adventitious bud induction culture medium added with screening antibiotics, and carrying out screening induction culture until the heredity-transformed regenerated adventitious buds of the cunninghamia lanceolata are obtained. The technical system can be widely applied to the field of genetic improvement of cunninghamia lanceolata, and a reliable technical platform is provided for efficient transformation of disease-resistant genes, stress-resistant genes and wood quality related genes.

The invention provides a cunninghamia lanceolata rapid propagation method using stem segments as explants, and belongs to the technical field of plant tissue culture, the method comprises the following steps: S1, using the stem segments of cunninghamia lanceolata as the explants, and inoculating the stem segments on a first culture medium for induction culture; s2, inoculating the adventitious buds obtained in the step S1 to a second culture medium for multiplication culture; s3, inoculating the adventitious buds obtained in the step S2 into a third culture medium for rooting induction culture; s4, transplanting the aseptic seedlings obtained in the step S3; wherein the first culture medium and the second culture medium contain 6-BA and IBA, and the third culture medium contains SNP and NAA. According to the method, the tender stem of the cunninghamia lanceolata is taken as the explant, a cunninghamia lanceolata aseptic seedling micropropagation seedling rapid propagation system is successfully established for the first time, the transplanting survival rate is extremely high, and a reliable way is provided for keeping the excellent characters of the cunninghamia lanceolata and rapidly propagating the cunninghamia lanceolata.

The invention relates to the technical field of preparation and purification of active ingredients of natural products, and discloses a cryptomeria fortunei essence anti-hair-loss hair-nourishing scalp care agent and a preparation method thereof.The preparation method comprises the steps that cryptomeria fortunei raw materials are extracted in a low-temperature high-concentration ethanol water solution through ultrasonic assistance; then low-temperature pure water is added for high-speed dilution, and slightly soluble impurities are induced to be separated out into a solid state; the method comprises the following steps: removing solid medicine residues and separating out impurities through primary solid-liquid separation, adding biocompatible protein into clear liquid, adjusting the pH value to realize co-precipitation so as to remove soluble sensitizing impurities in a targeted manner, and finally, carrying out secondary solid-liquid separation to obtain the cryptomeria fortunei essence. Decoupling of the active matter and sensitization impurities in dissolution characteristics is achieved, degradation of heat-sensitive active matter caused by high temperature is avoided, and meanwhile the problems that the cost is high and the yield is low when high-similarity matter is separated through a traditional purification means are solved.

The invention discloses a method for improving the embryogenic ability of cedarwood based on 5-azacytidine, and belongs to the technical field of plant biology. The method comprises the following steps: carrying out dark culture on cunninghamia lanceolata embryogenic calluses in a proliferation culture medium containing 5-azacytidine; carrying out dark culture on the calluses in an induction culture medium to induce cotyledon cell embryos; and finally, carrying out illumination culture on the somatic embryos in a germination culture medium. According to the method, 5-azacytidine with a specific concentration is exogenously added in explant proliferation and induction stages, so that the cell DNA methylation level can be remarkably reduced, and the embryogenic state of the callus is effectively maintained. Practice shows that the method can significantly improve the proliferation efficiency (up to 2.6 times) and the somatic embryo occurrence rate (up to 2.9 times) of cunninghamia lanceolata calluses of different genotypes, and an effective scheme is provided for solving the technical problem that the somatic embryogenesis capacity of cunninghamia lanceolata declines.