182 results about "Cunninghamia" patented technology

The invention relates to a mountainous area non-reclaimed slope water-collecting and fertilizer-maintaining forest medicine ecological combined method and belongs to the technical field of plant cultivation. The method is the improved method and is characterized in that equidistant horizontal tabletops which are internally tangent and externally cushioned are overturned and dug on a slope along a contour line from top to bottom, the width of the tabletops is 0.5-1 m, the height of the tabletops is 30-50 cm and the depth of soil is 15 cm; multiple layers of water-collecting and fertilizer-maintaining horizontal closure tabletops are formed along the slope; the table distance is kept to be 20-30 cm when the tabletops are overturned and dug, and gentle slope shrub and herbaceous plant belts between the tabletops are reserved; alnus nepalensis or cunninghamia lanceolata or fir with the height being 1.0-1.5 m is transplanted along the tabletops according to the spacing in the rows and the spacing between the rows, wherein the spacing is 2*2 mm. Gentiana rigescen or salviae miltiorrhizae is selected to be planted for 3 years, paris polyphylla or rhizoma polygonati is selected to be interplanted for 3-5 years, or rhizoma panacis majoris or pinellia ternate which is resistant to shade and damp is selected continuously to be interplanted for 3-5 years, and circulation plantation of varieties is performed according to the man-made forest plantation period or formed shading degree conditions. The mountainous area non-reclaimed slope water-collecting and fertilizer-maintaining forest medicine ecological combined method has the advantages of being easy to operate, low in cost, easy to popularize and friendly to the environment. A good foundation is laid for the development of medicine under a forest, and good economic benefits, ecological benefits and social benefits are achieved.

The invention discloses a tissue culture and rapid propagation method for Cunninghamia lanceolate which is a main afforestation tree species specific to the southern China. At present, seedlings of the Cunninghamia lanceolate are mainly cultured with traditional methods such as cutting, grafting, seeding and the like, and defects that the seedling culture cost is high, seedlings grow poorly and are irregular, the potential of improved varieties is not fully played and the like exist. According to the method, excellent clone twigs are used as explants, in-vitro regenerated plants of the Cunninghamia lanceolate are obtained through processes of explant disinfection, induction culture, cluster bud propagation, adventitious root taking, acclimatization, transplantation and the like, a tissue culture and rapid propagation technology system for the Cunninghamia lanceolate is established, excellent characteristics of clone female parents of the Cunninghamia lanceolate can be kept, and mass production of excellent clone seedlings of the Cunninghamia lanceolate is facilitated.

The invention discloses a somatic embryogenesis method for cunninghamia lanceolata, which comprises the steps of initial induction of an embryogenic suspensor cell mass, maintenance and multiplication of an ESM (embryogenic suspensor mass), and development and maturity of a somatic embryo, wherein the development and maturity of the somatic embryo can adopt an indirect somatic embryogenesis way or a direct somatic embryogenesis way. With the adoption of the somatic embryogenesis method for the cunninghamia lanceolata, browning in an establishment process of the conventional liquid suspension system can be effectively improved, cell division under a low density condition is started, and the stability of suspensor cells is maintained, so that a stable multiplication liquid suspension cell line is established. The somatic embryogenesis frequency of the cunninghamia lanceolata can be increased by about 20 times at most through the direct and indirect somatic embryogenesis ways, the problem of low somatic embryogenesis frequency caused by an incomplete somatic embryogenesis system of the cunninghamia lanceolata can be well solved, the cost is saved, conditions are prepared for factory production of the cunninghamia lanceolata, and a pace of industrial production of the cunninghamia lanceolata is accelerated.

The invention discloses a rooting induction method of a test-tube plantlet of cunninghamia lanceolata. The rooting induction method comprises the steps of: cutting a 1-2cm multiplication subculture plantlet, inoculating in a pre-rooting culture medium for culturing, wherein the pre-rooting culture medium uses 1/2MS-MS as a base culture medium, and also comprises 0.02-0.08mg/L of NAA (Naphthalene Acetic Acid), 2.5 percent of cane sugar and 0.7 percent of agar; carrying out rooting culture after pre-rooting and culturing for 15-25 days, wherein a rooting culture medium uses improved 1/2 MS as a basic culture medium, and also comprises 0.10-0.25mg/L, 0.05-0.08mg/L of IAA (Indole-3-Acetic Acid), 2.5 percent of cane sugar and 0.7 percent of agar; and placing in a proper environment for culturing to obtain a rooted test-tube plantlet. After the cunninghamia lanceolata is rooted and cultured by adopting the rooting induction method, the rooting rate, the root system quantity and the root system germination uniformity of the test-tube plantlet of the cunninghamia lanceolata can be increased, more excellent rooted test-tube plantlets are provided for production and application, and good economic benefit and social benefit are obtained.

The invention discloses a production method used for producing cunninghamia lanceolata biological essential oil from cunninghamia lanceolata saw dust. According to the production method, cunninghamia lanceolata saw dust is delivered into a vertical distillation tank, boiler steam is delivered into the vertical distillation tank from the bottom, after three-stage distillation, obtained essential oil is collected from a distillation outlet pipe on the upper part of the vertical distillation tank together with water vapor, and is delivered into a condenser, and cunninghamia lanceolata biological essential oil is obtained oil-water separation after cooling. According to the production method, waste saw dust generated in wood processing is used; the cunninghamia lanceolata biological essential oil is extracted via fine processing; enterprise economic benefit is improved; discharge of harmful gas is reduced; and the ecological environment is protected.

The invention provides a preparation method of a traditional Chinese medicine for treating meat-accumulation type hyperlipidemia and belongs to the technical field of traditional Chinese medicine preparation methods. At present, hypolipidemic is commonly used for treating the meat-accumulation type hyperlipidemia, which easily causes adverse effects. The technical scheme of the invention is as follows: the method comprises the following steps: soaking hawthorns, malts, rice sprouts, endothelium corneum gigeriae galli, buckwheat, medicated leaven, radish seeds, papaya, quince, greater celandine herb, elecampane, cunninghamia lanceolata, citrus chirocarpus, clove, fructus akebiae, truestar anisetree, kamuning, aspongopus, semen canavaliae, elecampane, szechwan chinaberry fruit, caesalpinia sepiaria, radix Linderae, rhizoma nardostachyos, rhizome, ambergris, radix aucklandiae and radix liquiritiae in water; decocting with slow fire, filtering the decoction with gauze to obtain a liquid medicine, namely the traditional Chinese medicine for treating meat-accumulation type hyperlipidemia. The traditional Chinese medicine for treating meat-accumulation type hyperlipidemia provided by the invention has the advantages that the traditional Chinese medicine has small side effect and small toxicity, is well absorbed in a digestive tract after oral administration, and is stable in curative effective and ensures that the possibility of meat-accumulation type hyperlipidemia reoccurrence is small.

The invention discloses a mutation breeding method for cunninghamia lanceolata. The method comprises the following steps: especially adopting ripen seeds of a cunninghamia lanceolata seed garden; carrying out low-temperature overwintering treatment, carrying out constant-temperature seed soaking stimulation on a gibberellin solution with specific concentration, immediately putting the gibberellin solution subjected to constant-temperature seed soaking stimulation into clear water, carrying out variable-temperature soaking for 3 days, and carrying out sprout promoting treatment; respectively carrying out colchicine mutation treatment, ethylmethane sulfonate (EMS) mutation treatment and sodium azide (NaN3) mutation treatment; and finally seeding and germinating the cunninghamia lanceolata seeds which are subjected to mutation treatment, so as to obtain a large group of cunninghamia lanceolata mutation seedlings. The cunninghamia lanceolata mutation seedlings obtained by the mutation method have the characteristics of obvious phenotypic characteristic variation, large population growth characteristic variation range and the like; a great number of mutated cunninghamia lanceolata materials can be formed within a short period of time by the method disclosed by the invention; and a foundation is laid for breeding of a new variety of cunninghamia lanceolata.

The invention relates to a tea interplanting method which refers to a method of interplanting vetches, tea trees and cunninghamia lanceolata in hillside woodland. The method particularly includes steps of hillside woodland modification, tea plantation and fertilization, cunninghamia lanceolata and milk vetch interplanting and the like. The cunninghamia lanceolata trees form a fence outside a ditch, and large tea seedlings which are planted inside can grow well due to the fact that tea trees are sciophilous. Therefore, the whole economic benefit can be increased by means of multi-crop interplanting.

The invention discloses a microorganic aerobic composting method of kitchen garbage. The microorganic aerobic composting method comprises the following steps: water is used for cleaning kitchen waste so as to obtain an oil-water mixture and solid garbage; wood chips and alicyclobacillus are added into the solid garbage, and then aerobic composting is carried out so as to obtain a composting product. According to the invention, reducing, harmless and resourceful treatment to kitchen waste is carried out by adopting a microbic aerobic composting way. According to the invention, oil-water separation and reverse osmosis water treatment methods are adopted for treating kitchen waste, so as to reduce oil components in the kitchen waste and improve the composting effect, and the water resource reutilization is achieved. In the aerobic composting treating process, carbon-nitrogen ratio and water content are adjusted by adding cunninghamia lanceolata chips, the high-temperature-resistant and acid-proof alicyclobacillus are used, during linked optimization processes of heating, stirring and time controlling, energy conservation is achieved, the treatment efficiency is improved, the composting product meeting national standards and industrial standards is produced, and requirements of environment protection and sustainable development are met.

The invention relates to the technical field of plant propagation, and provides a method for constructing a cutting orchard for a crowned trunk embedding method of cunninghamia lanceolata. The methodcomprises the following steps: selecting a nursery ground, carrying out soil preparation and applying a base fertilizer, planting seedlings, shaping the scion cutting mother trees, constructing a scion cutting surface and carrying out hormone treatment, and constructing a photosynthesis surface and carrying out hormone treatment. The method solves the problems that the scion quality is poor, the rejuvenation degree is low, the yield is low, and the service life of the cutting orchard is short in other types of cunninghamia lanceolata cutting orchards.

The invention discloses a breeding method for gastrodia elata. After cunninghamia lanceolata sawdust and shale-free sand are uniformly stirred, the stirred substance is sprayed with water to be wet and the wet substance replaces sandy soil as a substrate, so that the weight of each breeding bag filled with gastrodia elata seeds is reduced from 15-20kg to 7-8kg, the labor intensity is reduced, and the cost input is reduced; when the breeding bags are placed under shading woods, no covering is needed, the management is facilitated, and the yield is stable; the yield of the gastrodia elata is increased from about 0.2kg of each bag on average in the past to over 0.8kg of each bag, and planting benefits are remarkably improved.

The invention relates to an interplanting method of selenium-rich tea. The interplanting method is one for interplanting osmunda japonica thunb, tea and cunninghamia lanceolata in a hillside woodland, and particularly comprises the following steps: improving the hillside woodland, planting the tea, applying a selenium-rich fertilizer, interplanting cunninghamia lanceolata trees and astragalus smicus. The cunninghamia lanceolata trees are planted at the outside of a ditch to form a fence; the larger tea seedlings are planted on the inner side of the trees; the tea trees are suitable for the shady environment, so that the tea trees can grow well; the multi-crop interplanting can improve the whole economic benefit.

The present invention relates to a psoriasis treating external application traditional Chinese medicine ointment. The preparation method comprises: screening coptis chinensis, scutellaria baicalensis, cortex phellodendri, dandelion, sophora flavescens ait, spina gleditsiae and cortex dictamni according to a certain weight part ratio, removing impurities, washing, soaking for 2-3 h with water, placing into an autoclave, cooking for 1-2 h, adding a right amount of water to boil, filtering the drug liquid after 1-2 h, placing the drug liquid into an iron pot, decocting with mild fire until 100 g of the drug liquid is retained for spare, and uniformly mixing and stirring cunninghamia lanceolata oil, sesame oil, benzoic acid, salicylic acid, borneol, medical vaseline and the drug liquid so as to prepare the psoriasis treating external application traditional Chinese medicine ointment. The psoriasis treating external application traditional Chinese medicine ointment of the present invention has effects of rapid killing of stubborn fungi and bacteria, and skin infection eliminating, is suitable for all types of psoriasis, has characteristics of significant treatment effect, high cure rate, external application ointment, easy use, rich drug source, simple preparation method, low cost, smooth skin after cure, no cicatrice, no injury on human body, no pain, and no toxic-side effect.

The invention discloses a tea saponin containing special fertilizer for cunninghamia lanceolata. The special fertilizer comprises the following effective components in percentage by weight: 5.1-14.2% of N, 6.9-11.7% of P2O5, 7.5-20% of K2O, 8.0-21.25% of organic matters, 1.14-3.0% tea saponin, 0.00-0.46% of water-soluble Si, 0.00-0.37% of Zn, 0.00-0.24% of B and 0.00-0.3% of Cu. The special fertilizer disclosed by the invention can meet the nutrients needed for the growth of the cunninghamia lanceolata planted in Guangxi province, is rich in organic matters, contains tea saponin and trace elements, has the functions of improving the efficiency of photosynthesis, enhancing the disease and insect resistance and the drought resistance of the cunninghamia lanceolata, increasing the utilization rate of phosphorus and improving the soil, releases nutrients slowly and also has the function of comprehensively balancing and regulating the distribution of nutrients in the cunninghamia lanceolata. In addition, the special fertilizer has high utilization rate and has the functions of improving the yield and the quality of the cunninghamia lanceolata.

The invention discloses Chinese herbal bait. The Chinese herbal bait comprises the following ingredients in parts by weight: 15-30 parts of radix aconiti, 10-20 parts of chaulmoogratree seed, 10-20 parts of semen torreyae, 30-45 parts of dalavay silene root, 12-18 parts of fern leaves, 12-18 parts of cunninghamia lanceolata cones, 30-45 parts of thunder god vines, 15-25 parts of cortex meliae, and 40-45 parts of a carrier. The Chinese herbal medicine bait is prepared by a traditional technology. The Chinese herbal bait has the characteristics of being strong in termite attraction force and large in termite feeding amount, having an obvious killing effect, and not polluting the environment.

The invention relates to an insect damage prevention type tea planting method which refers to a method of interplanting vetches, tea trees and cunninghamia lanceolata in hillside woodland. The method particularly includes steps of hillside woodland modification, tea plantation and fertilization, cunninghamia lanceolata and ryegrass interplanting and the like. The cunninghamia lanceolata trees form a fence outside a ditch, and large tea seedlings which are planted inside can grow well due to the fact that tea trees are sciophilous. Therefore, the whole economic benefit can be increased by means of multi-crop interplanting.

The invention discloses a stress-resistance and health-care mycorhiza microbial inoculum of Cunninghamia lanceolata and an application method of the stress-resistance and health-care mycorhiza microbial inoculum. The stress-resistance and health-care mycorhiza microbial inoculum is prepared from a mycorhiza microbial inoculum and naphthylacetic acid by compounding; the mycorhiza microbial inoculum is formed by fermenting Lactarius subdulcis or Lactarius akahatsu or Lactarius hatsudake through a solid fermentation culture medium. The stress-resistance and health-care mycorhiza microbial inoculum is used for carrying out slurry dipping treatment on seedling root systems or is buried into Cunninghamia lanceolata forest soil in a forestation process, so that not only can root-taking and growth of the Cunninghamia lanceolata be increased, but also Cunninghamia lanceolata vigor is enhanced and the stress resistance is improved; and therefore Cunninghamia lanceolata yellows, seedling damping-off and Pestalotiopsis apiculatus are prevented from happening.

The invention relates to a rooting culture and hardening seedling method of a cunninghamia lanceolata tissue-cultured seedling. The method comprises steps of selection of tissue-cultured bud seedlings, rooting and inoculation, rooting culture, rooting and cap-sealed hardening seedling, and uncapped hardening seedling. The method combines rooting with hardening seedling, short-period rooting culture is carried out in a culture room, and rooting and cap-sealed hardening seedling and uncapped hardening seedling culture are carried out, so that the tissue-cultured seedling is subjected to rooting and hardening seedling at the same time. Time for rooting and hardening seedling of the cunninghamia lanceolata tissue-cultured seedlings is obviously shortened. Compared with a conventional method, the method allows the seedling culture period to be obviously shortened, provides suitable root length, and obviously improves the seedling culture efficiency. Energy and expense saving is achieved through natural temperature control and light control. The problems of high cost and low survival rate of transplanting in a production process of a cunninghamia lanceolata tissue-cultured seedling are solved. The method is beneficial for establishing an excellent asexual seedling culture base for cunninghamia lanceolata and provides superior seedlings for forestry production.

The invention provides a Ganoderma lucidum cultivation method using Cunninghamia lanceolata sawdust. The method comprises the steps of compost preparation, inoculating box and inoculating chamber disinfection, bagging, sterilization, hypha management, contaminating microorganism inspection and control, fruiting body growing, harvesting and the like. The Cunninghamia lanceolata sawdust is used as a raw material for essential oil extracting, cedar wood oil is replaced by essential oil which is used for the flavor industry, and the Cunninghamia lanceolata sawdust after essential oil extracting is used for Ganoderma lucidum cultivation. In the way, not only can the Cunninghamia lanceolata sawdust be used for food preservation and freshness retaining and the raw material of the flavor industry, but also waste is used, and resources are saved, so that environment protection is promoted.

The invention discloses a multifunctional health-care purple sand filtering water-purifying material. The purple sand filtering water-purifying material is composed of the following raw materials: 40-50 wt% of purple sand ore, 20-25 wt% of composite mineral calcined particles, 7-10 wt% of bamboo charcoal, 12-17 wt% of coconut shell powder, 2-4 wt% of cunninghamia lanceolata sawdust, 2-3 wt% of calcite, 1-2 wt% of sodium humate and 0.1-0.3 wt% of CMC., wherein the composite mineral calcined particles are composed of the following raw materials: 35-45 wt% of medical stone, 30-40 wt% of Muyu stone, 10-15 wt% of selenium-rich rock powder, 10-15 wt% of tourmaline and 5-10 wt% of monazite. In addition, the invention further discloses a preparation method of the purple sand filtering water-purifying material. The water quality is promoted to become slightly alkaline water while water is filtered and purified, and the water purifying material is rich in various minerals beneficial to human bodies, so that the use requirements of healthy drinking water of people are well met.

The invention discloses a preparation method of a cunninghamia lanceolata synthetic seed. The preparation method comprises the following steps: (1) using buds obtained by short stem multiplication in a cunninghamia lanceolata tissue culture process as propagules, wherein the bud multiplication culture medium is prepared from MS, 0.3 mg/L of IBA, 0.4 mg/L of 6-BA, 0.2 mg/L of VC, 30 g of sugar, 6.0 g of agar and 0.5 g of Ac; (2) preparing a seed embedding matrix with 1.5% of CaCl2, 1% of sodium alginate and agar according to complexing time of 5 minutes, after complexing, washing the seed embedding matrix with distilled water, adding 0.5% of agar into the embedding matrix, drying the water on the surface, sealing, and placing the embedding matrix into a refrigerator at the temperature of 4 DEG C. According to the preparation method disclosed by the invention, the sources of raw materials are accessible; the preparation method is simple and convenient to operate; compared with a test-tube plantlet, the cunninghamia lanceolata synthetic seed is small in size, convenient to transport and store and low in cost; the preparation method lays a foundation for popularizing and applying the cunninghamia lanceolata synthetic seed.

The invention relates to a biomass fuel with swine excrement and a preparation method thereof. The fuel is prepared from the following raw materials in parts by weight: 100 parts to 120 parts of the swine excrement, 50 parts to 60 parts of sawdust powder, 30 parts to 40 parts of branches and leaves of cunninghamia lanceolata, 30 parts to 40 parts of branches and leaves of pine wood, 20 parts to 30 parts of straw fermented materials, 10 parts to 20 parts of coal dust and 10 parts to 20 parts of clay. The preparation method of the biomass fuel comprises the following steps: mixing the branches and leaves of the cunninghamia lanceolata, the branches and leaves of the pine wood and the sawdust powder to obtain a mixture 1; then mixing the mixture with the swine excrement and the straw fermented materials to obtain a mixture 2; then mixing the mixture 2 with the coal dust, the clay and the remaining sawdust powder and uniformly stirring; drying and then feeding into a granulating machine; extruding into cylindrical grains; finally baking the cylindrical grains to obtain the biomass fuel. According to the biomass fuel prepared by adopting the method disclosed by the invention, the swine excrement is developed as the fuel, so that the cost of a farm is reduced, the problem of the shortage of the fuel also can be solved, treatment ways of the excrement is added, the waste of the excrement is avoided, and the fuel can be supplied while environment protection can be achieved at the same time; moreover, the biomass fuel has significant economic benefits and environmental benefits.

The invention discloses a culture method of cunninghamia lanceolata callus. The culture method comprises the steps of: screening cunninghamia lanceolata seeds with filled particles and without pests as explants, carrying out sterile culture to obtain sterile seedlings, clipping leaves, stems or root systems of the sterile seedlings, inoculating in an induction culture medium for inducing callusogenesis, and finally transferring the callus formed by induction into a proliferation medium for proliferation. The culture method is simple and easy to operate, is capable of effectively shortening the induction and proliferation time of the callus and increasing the induction rate of the cunninghamia lanceolata callus, provides a large quantity of excellent callus materials with good colors and compact structures for the development of the cunninghamia lanceolata industry, and has a good economic benefit.

The invention provides a screening method for reference genes in different tissues of cunninghamia lanceolata and application of the reference genes and belongs to the field of fluorescent quantitation PCR (Polymerase Chain Reaction) detection. The screening method comprises the following steps: taking a glyceraldehyde-3-phosphate dehydrogenase gene, a transcriptional elongation factor, 18S rRNA,28S rRNA and a ubiquitin gene as candidate reference genes, and using the candidate reference genes as template design primers; extracting total RNA of roots, stems and leaf tissues of cunninghamia lanceolata seedlings and synthesizing cDNA with the total RNA as a template; carrying out qPCR amplification with the cDNA as the template to obtain a Ct value and relative expression Q; carrying out stability evaluation on the candidate reference genes by adopting a plurality of software according to the relative expression Q value and Ct value; sorting the stability of the candidate reference genes from strong to weak, and selecting candidate genes ranking the top two as the reference genes of the cunninghamia lanceolata. The 18S rRNA and/or ubiquitin gene obtained by screening is applied as the reference gene in different tissues of the cunninghamia lanceolata.