38 results about "Infection time" patented technology

The invention provides a method of full-artificial culturing Cordyceps sinensis, including sampling, strain separating, host insect collecting, breeding and feeding, infecting and continuing feeding, on artificial condition, largely culturing Hirsutella sinensis; feeding host insects in an scale room at low altitude, completing the infection of Hirsutella sinensis to the host insects and sexuality of Cordyceps sinensis. By artificial culture, the host grubs are big and good-quality, and the success rate of infection is high, the infection time is short, the quality is stable and controllable, and the pesticide effect test fully indicates that the pesticide effect of the produced Cordyceps sinensis is equivalent to that of the natural Cordyceps sinensis and it can overcomes the disadvantages of long growing time of natural Cordyceps sinensis and low natural infection ratio, and implements the industrialized production of Cordyceps sinensis.

The invention belongs to the antiviral field of medical chemistry and relates to a novel pyridine derivative shown in a formula (I), a stereisomer, pharmaceutical salt, a solvent compound or a crystalof the pyridine derivative and an application of the pyridine derivative and the stereisomer, pharmaceutical salt, the solvent compound or the crystal in preparing drugs for preventing or treating viral infection diseases such as influenza A and/or influenza B, particularly in preparing a cap dependent endonuclease inhibitor in preventing or treating influenza A and/or influenza B viral infectiondiseases. The compound has remarkable activity for inhibiting influenza endonuclease and influenza DNA, can be independently used or can be combined with a neuraminidase inhibitor, nucleoside medicines, a PB2 inhibitor, a PB1 inhibitor, an M2 inhibitor or other anti-influenza drugs, the influenza infection time is notably shortened, the death rate is remarkably reduced, and the pyridine derivative has very good clinical application prospects.

The invention provides an anti-infective medical polymer material, comprising 100 dose of heat curable silicone rubber, 2.5 to 15 dose of Na-montmorillonite, 0.75 to 6.2 dose of quaternary ammonium salt intercalation compounds, 0.3 to 5.6 doses of antibiotics. Preparation method of the materials is: convert the montmorillonite clay to Na-montmorillonite, and then intercalate quaternary ammonium salts to montmorillonite to obtain the organic-montmorillonite with anti-infective drugs attached to it, and compound with heat curable silicone rubber at last. The material has a long anti-infection time, good physical and mechanical properties, and not easy to age. It can be used in the preparation of various medical catheters, such as catheters, deep venous catheters, drainage tubes, trachea intubation and so on.

The invention discloses a method for inducing and quickly propagating hairy roots of tartary buckwheat. The method includes (1), pre-cultivating sterile explants of the tartary buckwheat; (2), infecting the pre-cultivated explants of the tartary buckwheat by the aid of agrobacterium rhizogene infection liquid; (3), co-cultivating the infected explants; (4), transferring the co-cultivated explants into induction cultivation media and carrying out induction cultivation on the co-cultivated explants; (5), carrying out secondary cultivation and amplification cultivation on the hairy roots of the tartary buckwheat. The hairy roots of the tartary buckwheat are generated by means of induction. The method has the advantages that key parameters such as the explant pre-cultivation time, the agrobacterium rhizogene infection liquid concentration, the infection time, the co-cultivation time and the types of agrobacterium rhizogene which can obviously affect the induction efficiency for the hairy roots of the tartary buckwheat are optimized, accordingly, effects of inducing the hairy roots of the tartary buckwheat can be obviously improved, and the hairy roots of the tartary buckwheat can be quickly and efficiently induced; the method is applicable to inducing hairy roots of different varieties of tartary buckwheat, materials are convenient to obtain, the method is high in applicability and induction speed and easy and convenient to implement, heritance is stable, the cost can be saved, and the like.

The invention discloses an abnormal user identification method suitable for being executed in computing equipment, and the method comprises the steps: collecting user identification records generatedby a plurality of pieces of equipment in a service request, each user identification record comprising a plurality of user identifications; generating a user identifier relation between every two useridentifiers in each user identifier record; generating a corresponding user relationship network by taking the user identifier as a network node and the user identifier relationship as a network path, the user relationship network comprising the reliability of the network node and the strength of the network path; identifying a normal network in the user relationship network, and monitoring the infection time and infection nodes when the normal network is infected as an abnormal network; and marking the user identifier corresponding to the infected node as an abnormal user identifier. The invention also discloses a corresponding abnormal user identification device and computing equipment, which can effectively distinguish a normal network from an abnormal network and solve the trouble ofabnormal identification on business and data processing.

The invention discloses a modeling method of a helicobacter pylori infection animal model. The modeling method comprises the steps of: 1, by using a 4-weeks old female C57BL/6 mouse as the experimental animal, performing intra-gastric administration for 3 to 7 days by mixed antibiotic, wherein the mixed antibiotic is composed of gentamicin, vancomycin and nystatin; 2, raising the C57BL/6 mouse which has been subjected to the intra-gastric administration in the step 1, for 6 to 8 days; 3, inoculating helicobacter pylori: fasting the C57BL/6 mouse obtained in the step 2, carrying out intra-gastric administration by helicobacter pylori, and feeding after 8 to 12 hours, wherein the inoculating in the step 3 is performed one time every other day, and performed 2 to 4 times in total; and 4, raising the mouse which has been inoculated for 2 to 4 times in the step 3. In the modeling method provided by the invention, the experimental time is short, the operation time is less, the order of magnitude of the helicobacter pylori infection is high, and the infection time is endurable; the model can be widely used for the method of helicobacter pylori infection animal in related researches.

The invention discloses a method for efficiently and rapidly stabilizing gene transformation for tomatoes. The method comprises the steps that 1, activation of stains is conducted; 2, preparation of plant materials is conducted; 3, infection is conducted; 4, screening and culturing are conducted. According to the method, the tomato transgenic efficiency is increased, and the transformation rate can reach over 30 percent. The concentration of bacterial liquid is reduced, the infection time is prolonged, and on one hand, agrobacterium pollution is avoided; on the other hand, the infection efficiency is increased. The infection bacterial liquid is not added with drugs for assisting in infection, so that damage to tomato leaves is reduced, the survival rate and the regeneration rate of leaf culture are increased; in a traditional method, drugs such as acetosyringone which assists in infection are usually added, damage is caused to leaves, and the regeneration capacity of the leaves is reduced. By means of carriers carrying strong expressed reporter genes, transgenic plants can be obtained directly by screening through observation, and tedious work in traditional detection is avoided. The cycle of transgenes is shortened.

The invention discloses an induction and rapid propagation method of salvia chinensis hairy roots, belonging to the technology of biological culture. The method comprises the following steps: (1) infecting salvia chinensis explants with an agrobacterium rhizogenes infection solution; (2) co-culturing the infected explants; (3) transferring the co-cultured explants to an induction medium for induction culture; (4) performing degerming subculture and multiplication culture on salvia chinensis hairy roots generated by induction. Agrobacterium rhizogenes type, explant type and key parameters suchas pre-culture time, infection time and co-cultivation time of the explants, which significantly affect the induction efficiency of the salvia chinensis hairy roots, are optimized to significantly improve the induction effect of the salvia chinensis hairy roots, achieve rapid and efficient induction of the salvia chinensis hairy roots, and expand sources for obtaining cyclic peptide; the method has the advantages of convenience in material extraction, simpleness in operation, fast induction, genetic stability and the like.

The invention provides a method for evaluating a lung infection medicine. In the method, an acinetobacter baumannii lung infection model is constructed by an ultrasonic atomization method, bacterium quantitative culture and pathological inspection are carried out, and comprehensive evaluation is carried out according to various indexes, such as general situations, pathological changes, lung bacterium number changes and the like, of rats at different infection time. The method for evaluating the medicine, which is provided by the invention, has the advantages of strong practicability, very high repeatability, reliability, applicability and controllability of the model, very small difference between model rats, good stability of the model, high similarity degree with a clinical pathogenic process, high model formation speed and the like, has the most pivotal advantage of simple operation of the model constructed by the method, can carry out batch operation simultaneously and provides a basis for researching on acinetobacter baumannii lung infection pathogenic mechanism and sieving treating medicines.

The invention discloses an embedding method of an arbuscular mycorrhizal fungus and a preparation method of a capsule fungus. The invention uses sodium alginate as a basic carrier to spore, mycorrhizal and hyphae of the fungus Glomus intraroote Embedded in gel particles, the complete infection process can be completed when sown in non-sterilized soil, and the infection rate has a certain increase with the extension of the infection time. Chitosan, gelatin, glycerin, rice, peat, coal cinder, and malt root added different nutrients, so that the infection rate of the bacterial agent was different, and the infection rate of rice was the highest, reaching 42.9% after 25 days.

The invention discloses an agrobacterium-induced strawberry leaf transient gene expression method and an application of same, wherein the method is established through a vacuum-infection technology. In the method, a strawberry leaf serves as an infection material, and variety of the strawberry, seedling age of the strawberry leaf, leaf position, vacuum infection time, and growth culture medium type are optimized with yellow fluorescent protein YFP being a report gene, thereby obtaining the optimum conditions for expressing foreign proteins. The method is rapid and high-effective and can be applied to the fields of foreign protein expression, gene sub-cell positioning, promoter activity detection, resistance gene function verification, etc.

The invention relates to an agrobacterium tumefaciens mediated transformation system for efficiently obtaining transgenic plants of paspalum vaginatum and application thereof. The agrobacterium tumefaciens mediated transformation system adopts the following steps of: by an efficient regeneration system, obtaining an embryonic callus receptor material; culturing donor agrobacterium tumefaciens strains; determining ablastin cephalosporin concentration; optimizing agrobacterium tumefaciens transformation conditions; carrying out screening culture and plant generation of resistant calli and carrying out molecular detection of the transgenic plants. By optimizing influence factors of bacteria concentration, infection time, infection conditions, co-culture conditions and the like in the agrobacterium tumefaciens transformation process, the paspalum vaginatum agrobacterium tumefaciens mediated transformation system is established. The invention overcomes the defect that an existing agrobacterium tumefaciens mediated technology is difficult to apply to genetic transformation of warm-season turfgrass, and establishes the efficient paspalum vaginatum agrobacterium tumefaciens transformation system which uses embryonic calluses as the receptor material.

The invention provides a cell screening system for anti-SGIV drug screening based on cell activity analysis, comprising: serum concentration of 5%, when DMSO needs to be added as a solvent, the DMSO concentration is not higher than 1%, the multiplicity of infection of the virus is 0.3, and the virus The infection time is 48h. The invention also provides an optimization method of a cell screening system for screening anti-SGIV drugs and a drug screening method. The advantage of the method of the present invention is that the cytopathic effect (CPE) caused by quantitative SGIV infection can be quantified for the first time, the cell screening system for anti-SGIV drug screening is optimized, and a simple screening method is established using this system, using ddPCR for The detection of screening results is accurate and fast.

The invention discloses a method for genetic transformation of zoysia matrella mediated by agrobacterium tumefaciens. The method includes the steps of the concentration of a bacterial solution, the infection time, the co-culture time, the antibiotic concentration, acclimatization and transplantation, PCR detection of transgenic plants and the like. The method includes the following steps that an optimal genetic transformation system is that an OD600 value of the bacterial solution is 0.4, infection is conducted for 30 min, and selective culture is conducted after co-culture is conducted for 3d; the optimal bacteriostatic concentration of Timentin in a selective culture phase is 250 mg/L, the optimum selection pressure for hygromycin callus screening is 40 mg/L, and the optimum concentration of root taking screening is 15 mg/L; and histochemical analysis and PCR identification of GUS activity show that a target gene is successfully transferred into a genome of the zoysia matrella. Theabove research provides important technical support for gene function research and molecular breeding of the zoysia matrella. The method has the advantages that a stable and high-efficiency zoysia matrella genetic transformation system is obtained, the convenience is provided for genetic improvement of the zoysia matrella, and meanwhile, a key preliminary foundation is laid for verification of functional genes of the zoysia matrella.

The invention belongs to the technical field of virus gene recombination, and particularly relates to a method for inserting exogenous DNA fragments into a specific large DNA virus genome in a fixed-point, directional and efficient manner by utilizing a non-homologous recombination mechanism. The method comprises the following steps of 1) designing and constructing a CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9) system for a specific cleavage site of the DNA virus genome; 2) designing and constructing an exogenous gene donor carrying one or more same cleavage sites; 3) introducing an expression vector of the CRISPR-Cas9 system and the exogenous gene donor into a specific cell according to a certain proportion and sequence; 4) aftera period of time, according to virus characteristics, selecting a proper infection time point and multiplicity of infection, and infecting the cell with viruses; 5) selecting an optimized time point to collect progeny recombinant viruses generated after virus infection; and 6) separating, screening and identifying target recombinant progeny DNA viruses. By means of the method, the recombination efficiency of inserting an exogenous gene into the DNA virus genome in a fixed-point and directional manner can be remarkably improved, so that the construction and screening time of the recombinant viruses can be shortened.

The invention discloses a combination increasing the yield of alfalfa and improving the quality of alfalfa and application thereof. The combination used for preparing rhizobia culture media is composed of active components of components A and components B, the components A are prepared from stachydrine or hydrochloride of stachydrine, and the components B are prepared from trigonelline or hydrochloride of trigonelline. Alfalfa seed rhizobia seed coatings prepared from the combination used for preparing the rhizobia culture media increase the yield of medicago sativa L.cv.Zhongmu No.1 and improve the quality of the medicago sativa L.cv.Zhongmu No.1. Raw materials of the rhizobia seed coatings for the medicago sativa L.cv.Zhongmu No.1 are easy to obtain, the coating mode is easy to operate, coated seeds are suitable for storage, transportation and sowing, and the coating mode can provide favorable infection time and space for rhizobia infection root system inoculation.

The present invention includes the following steps: obtaining embryonic callus acceptor material via high frequency regeneration system, culturing donor strain, determining concentration of ablastin, optimizing agrobacterium transforming condition, screening and culturing transformed callus and molecular detection of plant regeneration and resisting plant. By means of optimizing the conditions for transforming agrobacterium, including bacterium liquid concentration, infection time, infection condition, co-culture time, ablastin concentration, etc., the present invention provides agrobacterium mediated transformation system suitable for perennial ryegrass. The present invention solves the technological problem of applying agrobacterium mediating technology in genetic transformation of gramineous plant and establishes efficient agrobacterium mediated transformation system with embryonic callus as acceptor material for perennial ryegrass.

The invention discloses a method for constructing an efficient genetic transformation system of lilium lancifolium, which comprises the following steps: obtaining explants, activating Agrobacterium, infecting the Agrobacterium and co-cultivating Agrobacterium, and inducing bud differentiation and rooting. The method adopts a 2-3mm thin slice of the sterile small scale of lilium lancifolium as a transformation material, and establishes the efficient genetic transformation system of lilium lancifolium by medium culture improvement and optimization of Agrobacterium liquid concentration and infection time. The instantaneous transformation efficiency of lilium lancifolium is more than 80%, the stable conversion rate is above 25.00%, the conversion efficiency is high, the bacteria are not easilystained, and the chimera is not easily produced. The method solves the problem that the sterile small scale is used as the explant or a tTCL technology is used alone, and the genetic conversion efficiency of the lilium lancifolium is low; at the same time, the method overcomes the defect that lilium lancifolium callus is used as a transforming receptor, and the callus is too sensitive to Agrobacterium to finally obtain the transgenic plants, and the method has important guiding significance and reference value for the construction of the genetic transformation system of Agrobacterium-sensitive lilium lancifolium.

The invention provides an automatic management method and system for the number of people in a hospital infection state in a specific time period. The method comprises the following steps: determiningan authority department of a user according to identity information of the user by utilizing transfer information, hospitalization process information, infection information, selected statistical time and department; acquiring infection time and return time of the patient, and constructing time period parameters corresponding to infection; according to the infection-related time period parametersand the start-stop time period list, screening the cases in the hospital infection state effectively; and respectively determining infection information of the bound scalpel and infection informationof the unbound scalpel, and combining the infection information of the unbound scalpel and the infection information of the bound scalpel to obtain infection diagnosis information. According to the method, the number of people in the hospital infection state can be automatically managed, the infection-related time period parameters and the start-stop time period list are fully utilized, the counted number of people in the hospital infection state is high in practicability, and the application value is high.

The invention belongs to the field of biological research and specifically relates to the establishment of a hepatitis B virus (HBV) infection model under a novel human liver biological scaffold three-dimensional culture system. According to the invention, the hepatitis B virus infection model is established through wide and deep research; the model has the main characteristics that the infectionefficiency is high, a viral load used in an infection process is much lower than that of other models, but the replication level of a virus in cells is high. Furthermore, the infection lasting time ofthe model is long, and specifically, the in vitro infection of common primary liver cells only can last for about 10 days, but the infection time of the model involved in the invention can last for nearly 20 days. In addition, the expression of the liver cells in the model involved in the invention is close to an in vivo real state and the model can be used for screening hepatitis B resisting medicines.

The embodiment of the invention relates to an infectious disease infection probability calculation method and device, a storage medium and electronic equipment, and relates to the technical field of big data processing. The method comprises the following steps: acquiring a movement track of an infectious disease patient in a preset time period, and segmenting the movement track according to a preset segmentation rule to obtain a plurality of track segments; determining an infection space-time range of each track segment, and obtaining a to-be-predicted object in each infection space-time range from preset space-time data; calculating the local infection probability of each to-be-predicted object according to the first distance between each to-be-predicted object and the infectious disease patient in each infection time-space range; and calculating a target infection probability of each to-be-predicted object on the moving track according to the local infection probability of each to-be-predicted object in each infection time-space range. According to the embodiment of the invention, the accuracy of the target infection probability is improved.

The invention provides an aquilaria sinensis hairy root induction and genetic transformation method. According to the method, on the basis of research on conditions such as explant types, infection modes, infection times and hairy root culture temperatures, conditions applicable to aquilaria sinensis hairy root induction and culture are tested and screened, and most appropriate infection conditions for different explants of aquilaria sinensis are obtained. In addition, optimal induction conditions for inducing hairy roots from different parts (cotyledon, stems, roots and leaves) of SW101 aquilaria sinensis are provided. The invention provides a method for improving aquilaria sinensis hairy root genetic transformation efficiency. A green fluorescent protein (fgp) is introduced into aquilaria sinensis hairy roots, whether aquilaria sinensis hairy root genetic transformation succeeds or not can be visibly identified, and meanwhile, the effectiveness of a transformation system can be doubly verified in a PCR (Polymerase Chain Reaction) detection mode. Good, efficient and simple options are provided for aquilaria sinensis function gene study and/or industrialization of aquilaria sinensis hairy root metabolite.

The invention provides a wheat genetic gene transformation method. After agrobacterium attaches to the wound tissue of plants, through the interaction between the agrobacterium and the wound tissue of the plants, agrobacterium can form small cellulose fibrils which are fixed to cell walls of the plants, meanwhile, the injured plant tissue is induced to release some phenolic compounds, such as acetosyringone (AS) and hydroxy acetosyringone (AS-OH), and the phenolic compounds are taken as signal molecules to activate the transcription of other Vir genes and activate T-DNA with target gene segments of meloidogynosis to be inserted into chromosomes of the callus. The result shows that the genotype is the key factor influencing the formation and differentiation and regeneration of the wheat explant callus; the factors including the concentration of inoculated bacterium fluid, the infection time, the co-culture conditions and the like have great influences on the genetic transformation efficiency of the wheat explant callus; the obtained transgenic wheat regenerated plant lays a solid foundation for the genetic transformation research of wheat and the genetic analysis for the transgenic progenies.