4results about How to "Short experiment time" patented technology

The invention relates to a method for determining silicon dioxide in quartz glass based on a high-temperature melting reaction-inductively coupled plasma atomic emission spectrometer. The method comprises the following steps: crushing and grinding a sample into powder; adding a strong alkali flux into the obtained powdery sample, and uniformly mixing to obtain a mixture; melting the mixture at high temperature; leaching reactants with hot water and fixing the volume; drawing a silicon element concentration-spectral line emission intensity standard curve; testing the content of silicon in the sample solution; and calculating the content of silicon dioxide according to the measured content of silicon. The method comprises the following steps: adding a strong alkali solvent into a powdery sample obtained by grinding the sample, carrying out a high-temperature melting reaction, heating water to dissolve a reactant, and determining the content of silicon in the solution by adopting an inductively coupled plasma atomic emission spectrometer, so as to calculate the content of silicon dioxide in the quartz glass sample.

The invention discloses an early warning evaluation method based on canceration caused by pulmonary nodule immune microenvironment imbalance, which comprises the following steps: S1, acquiring autoantibody detection data of normal, pulmonary nodule and lung cancer people from a database, and establishing a pulmonary nodule canceration data set Panne l containing 159 autoantibodies as risk early warning factors of pulmonary nodule canceration; s2, preparing a lung cancer risk factor Panne l protein chip according to the Panne l data, wherein the lung cancer risk factor Panne l protein chip comprises antigen proteins of 159 lung cancer related autoantibodies; s3, using a Panne l protein chip to detect the serum of the crowd; and S4, according to the detection data, establishing a risk early warning model of pulmonary nodule canceration for the analysis and early warning of the lung cancer occurrence risk of the crowd (mainly aiming at the pulmonary nodule crowd). According to the method, the cancer related autoantibodies are systematically detected through a protein chip method for people with high detection rate of pulmonary nodules in China, and the method is used for early warning of the occurrence risk of pulmonary nodule immune abnormality canceration of people.

The invention discloses a coal bed gas horizontal well drilling fluid plugging performance evaluation method, and relates to the technical field of oil and gas exploitation, coal dust and coal gangue returned by a coal bed gas horizontal well are taken, dried and crushed, a cementing agent is added, a glue-containing mixture is prepared, and curing and cutting are performed to obtain a coal rock plate or a coal rock plate with microcracks; a coal rock plate is used as a filtration medium of the plugging instrument, and the measured plugging filtration loss and the plugging filtration loss reduction rate are used as evaluation indexes for evaluating the coal rock plugging anti-collapse performance of the drilling fluid; a coal rock plate with micro-cracks is used as a filtration medium of the plugging instrument, and the measured plugging filtration loss and the plugging filtration loss reduction rate are used as evaluation indexes for evaluating the plugging anti-collapse performance of the drilling fluid on coal rock with micro-crack development, so that the anti-collapse performance of horizontal well water-based drilling fluid such as coal bed gas and the like can be reflected more reliably, quickly and accurately; the method is used for guiding safe and rapid construction of horizontal wells easy to collapse, such as deep coal bed gas and carbonaceous mudstone.

The present application belongs to the technical field of analytical chemistry, and particularly relates to a screening method for FcRn small molecule antagonists based on phase transition combined with FRET. In the present application, a fluorescent donor dye required in a FRET process is labeled on IgG, and a fluorescent acceptor dye is labeled on a phase transition material P4VP-SSO3Na-4VP; the phase transition material can reversibly change the phase when the environmental pH changes; when the material changes from a homogeneous system to a heterogeneous system, free IgG in the solution is embedded in the system, the distance between the fluorescent donor and the acceptor in the FRET process is shortened, and the fluorescent signal of the acceptor in the FRET system is detected; and further detection is made on whether the compound has antagonistic effect on FcRn, so as to screen FcRn small molecule antagonists. The present application overcomes the deficiency that some small molecule compounds have no suitable sites for dye labeling; has repeatability, low experimental cost, and can realize high-throughput and rapid screening of FcRn small molecule antagonists.