2results about How to "Short experiment time" patented technology

The invention discloses a coal bed gas horizontal well drilling fluid plugging performance evaluation method, and relates to the technical field of oil and gas exploitation, coal dust and coal gangue returned by a coal bed gas horizontal well are taken, dried and crushed, a cementing agent is added, a glue-containing mixture is prepared, and curing and cutting are performed to obtain a coal rock plate or a coal rock plate with microcracks; a coal rock plate is used as a filtration medium of the plugging instrument, and the measured plugging filtration loss and the plugging filtration loss reduction rate are used as evaluation indexes for evaluating the coal rock plugging anti-collapse performance of the drilling fluid; a coal rock plate with micro-cracks is used as a filtration medium of the plugging instrument, and the measured plugging filtration loss and the plugging filtration loss reduction rate are used as evaluation indexes for evaluating the plugging anti-collapse performance of the drilling fluid on coal rock with micro-crack development, so that the anti-collapse performance of horizontal well water-based drilling fluid such as coal bed gas and the like can be reflected more reliably, quickly and accurately; the method is used for guiding safe and rapid construction of horizontal wells easy to collapse, such as deep coal bed gas and carbonaceous mudstone.

The present application belongs to the technical field of analytical chemistry, and particularly relates to a screening method for FcRn small molecule antagonists based on phase transition combined with FRET. In the present application, a fluorescent donor dye required in a FRET process is labeled on IgG, and a fluorescent acceptor dye is labeled on a phase transition material P4VP-SSO3Na-4VP; the phase transition material can reversibly change the phase when the environmental pH changes; when the material changes from a homogeneous system to a heterogeneous system, free IgG in the solution is embedded in the system, the distance between the fluorescent donor and the acceptor in the FRET process is shortened, and the fluorescent signal of the acceptor in the FRET system is detected; and further detection is made on whether the compound has antagonistic effect on FcRn, so as to screen FcRn small molecule antagonists. The present application overcomes the deficiency that some small molecule compounds have no suitable sites for dye labeling; has repeatability, low experimental cost, and can realize high-throughput and rapid screening of FcRn small molecule antagonists.