5results about How to "Simplify the experimental process" patented technology

This invention discloses a sequencing technique for enriching mutations after library construction. The co-priming amplification (Co-PCR) technique of this invention couples a mutation probe to a short P5 / P7 primer. This short primer has a low melting temperature (Tm) and cannot hybridize and amplify on its own at the reaction temperature. Only when the mutation probe hybridizes to the mutation site can it assist the coupled short P5 / P7 primer in hybridizing to the adjacent P5 / P7 sequence at the end of the pre-library, initiating amplification. Therefore, this Co-PCR technique not only amplifies a specific mutation in a specified gene but also amplifies from both ends of the original pre-library, replicating the complete sequence information of the pre-library, including sequencing adapters, indexes, and sequencing primers. The amplified products can be directly sequenced after purification. By optimizing the co-primers, Co-PCR enrichment amplification of five sites in the pre-library was achieved, resulting in nearly 100-fold enrichment.

The utility model relates to chemical analysis instrument technical field, concretely is a kind of automatic titration device, including support, reagent bottle is equipped with inverted installation on support, the convex peak that can be inserted into reagent bottle and multiple pipes on connecting convex peak, multiple first pipe holes are equipped on the convex peak, the inside of support is equipped with supporting plate, multiple second pipe holes are equipped on supporting plate, one end of pipe is connected first pipe hole, and the other end is connected titration tube through second pipe hole, color sensor is equipped above each titration tube, conical flask is equipped below each titration tube, magnetic stirrer is equipped below each conical flask, device supports multichannel synchronous operation, end point color can be calibrated storage, solve artificial titration end point determination error and fatigue problem, significantly improve the accuracy and efficiency of batch detection.

The present application belongs to the field of precision measurement, and particularly discloses a method for measuring the transverse temperature of an atomic group in an atomic interferometer. The present application first controls the size of the atomic group at the moment of acting on the Raman light pulse by adjusting the opening time, and then performs a Rabi oscillation experiment under different atomic group sizes. Then, the experimental data are fitted with a trigonometric function, and the key parameters reflecting the transverse temperature are extracted therefrom. Finally, the transverse temperature of the atomic group is obtained by inversion calculation. The method is based on the Gaussian distribution of the atomic group density and the Raman light intensity in the transverse space, and the coupling result of the two is the non-uniformity of atomic transition in the transverse space, thereby affecting the average transition probability of the whole atomic group, i.e. the linearity of the Rabi oscillation curve. Conversely, the linearity of the Rabi oscillation of the atomic group contains the transverse parameters of the atomic group, and the transverse parameters of the atomic group are extracted from the linearity of the Rabi oscillation. The present application can be completed only by relying on the atomic interferometer itself, and has low requirements for equipment, simple process and easy operation.

The application discloses a universal programmable segmented linear function simulation circuit and relates to the technical field of electronic information engineering, comprising a judgment module, a selection module and an addition module; the judgment module comprises multiple judgment branches, each of which comprises a comparator and a threshold voltage, the noninverting input end of the comparator is connected to an original input signal, and the inverting input end is grounded through the threshold voltage; the selection module comprises a controllable bias voltage and analog switches equal in number to the judgment branches, the control ends of the analog switches are connected to the output ends of the comparators one by one, and the input ends of all the analog switches are grounded through the controllable bias voltage; the addition module comprises an inverting adder and an input resistor, the input end of the inverting adder is connected to the output ends of all the analog switches and is connected to the original input signal through the input resistor, and the output end of the inverting adder serves as a circuit output. The application simulates a segmented linear function and has the characteristics of universality, online programmability and easy expansion.

This invention relates to the field of marine clean energy simulation technology, and discloses a rapid indoor simulation non-destructive evaluation method for hydrate-containing sediments, comprising the following steps: S1, preparing an indoor simulation system for simulation: the indoor simulation system includes a sample saturation component, a sample component, an acoustic testing component, a temperature control component, and a data acquisition component; S2, preparing and saturating the sample. This invention can rapidly generate hydrates and directly prepare saturated sediment samples under indoor conditions, significantly shortening the sample preparation cycle; it avoids hydrate decomposition or structural disturbance during saturation, improving sample structural stability and experimental repeatability; the sample remains within the ring cutter throughout the preparation and testing process, eliminating the need for demolding and significantly simplifying the experimental procedure; it achieves non-destructive evaluation of hydrate saturation through acoustic testing, and can combine temperature monitoring to reflect the internal phase transition process and latent heat effect of the sample in real time.