13results about How to "Simplify the experimental process" patented technology

The application provides a construction of a fluorescent protein labeled microtubulin and a microtubule binding protein universal bivalent carrier, and the process is as follows: a first stage is to construct a GFP-alpha tubulin carrier, and a second stage is to construct a GFP-alpha tubulin-mCherry universal bivalent co-expression carrier; in the second stage, 35S, mCherry and NOS sequences are inserted into a KpnI enzyme cutting site of a multiple cloning site of the GFP-alpha tubulin carrier constructed in the first stage, and a single nucleic acid enzyme cutting site is inserted into the 5' end and the 3' end of the mCherry sequence, respectively, and the single nucleic acid enzyme cutting site is XbaI, KpnI / Acc65I and AscI sequences, respectively, so as to obtain the universal bivalent carrier. The universal bivalent carrier provided by the application can express two target genes simultaneously, has the fluorescent signals of GFP and mCherry, can quickly and accurately identify a transgenic plant, is convenient for positive seedling screening, can be used for a tobacco transient expression experiment, can shorten an experimental period, and saves time and effort.

This invention discloses a sequencing technique for enriching mutations after library construction. The co-priming amplification (Co-PCR) technique of this invention couples a mutation probe to a short P5 / P7 primer. This short primer has a low melting temperature (Tm) and cannot hybridize and amplify on its own at the reaction temperature. Only when the mutation probe hybridizes to the mutation site can it assist the coupled short P5 / P7 primer in hybridizing to the adjacent P5 / P7 sequence at the end of the pre-library, initiating amplification. Therefore, this Co-PCR technique not only amplifies a specific mutation in a specified gene but also amplifies from both ends of the original pre-library, replicating the complete sequence information of the pre-library, including sequencing adapters, indexes, and sequencing primers. The amplified products can be directly sequenced after purification. By optimizing the co-primers, Co-PCR enrichment amplification of five sites in the pre-library was achieved, resulting in nearly 100-fold enrichment.

The invention discloses an RNA (Ribonucleic Acid) target region enrichment method based on a circular probe, and belongs to the technical field of biomolecule detection and gene sequencing. The method comprises the following steps: designing an annular probe containing an anchoring sequence, a UMI sequence and a universal primer sequence (at least one anchoring sequence 5'end is phosphorylated and modified); the method comprises the following steps: hybridizing a probe with target RNA (Ribonucleic Acid), enriching a hybridization compound through oligo d (T) magnetic beads, cyclizing the probe through DNA ligase, and carrying out PCR (Polymerase Chain Reaction) amplification to obtain an enriched product. Reverse transcription and extension steps are not needed, a solid-phase substrate is not needed, the experimental process is simplified, and the operation time is shortened; through combination of specific probe design and magnetic bead enrichment, high-selectivity enrichment of target RNA is realized, the method is especially suitable for differential expression analysis of samples with low expression quantity, and the template recovery rate and the effective data proportion are effectively improved.

The invention discloses a reconstruction method and a reconstruction device of a space protein expression map and a storage medium. Comprising the following steps: receiving a multi-channel high-resolution tissue image of a tissue region obtained based on tissue slices; receiving a polymerization true value of the expression quantity of each target protein on each first-direction tissue band and each second-direction tissue band, wherein the polymerization true value is obtained after the first-direction tissue band and the second-direction tissue band are cut on the first tissue slice and the second tissue slice; a deep learning network is trained based on the aggregation true values of the protein expression quantities on the tissue strips in the first direction and the second direction and the multi-channel high-resolution tissue image, and the trained deep learning network predicts the expression quantity of each target protein on each image unit based on the multi-channel high-resolution tissue image; and reconstructing a two-dimensional protein expression map. According to the method, the two-dimensional space protein map can be reconstructed with high precision only by using as few as two tissue slices and the aggregation value of the protein expression quantity on the tissue strip which is relatively easy to obtain without the protein expression quantity of each unit.

The utility model belongs to the field of atom inertia measurement, and particularly discloses an auxiliary device for evaluating the Coriolis force of an atom interferometer, the auxiliary device comprises a lifting rotation supporting module and an inclination detection adjusting module, and the inclination detection adjusting module comprises an inclination detection unit and an inclination adjusting unit; the inclination detection unit is used for detecting the inclination of the vacuum container; the inclination adjusting unit is located at the bottom of the vacuum container and used for supporting the vacuum container and adjusting the vacuum container to be horizontal according to the inclination. The lifting rotation supporting module can be coaxially arranged under a vacuum container of the atom interferometer and can drive the vacuum container to rotate so as to modulate the Coriolis force. According to the invention, the effect of rotating the vacuum container in the atom interferometer with high efficiency and high precision can be realized.

The utility model relates to chemical analysis instrument technical field, concretely is a kind of automatic titration device, including support, reagent bottle is equipped with inverted installation on support, the convex peak that can be inserted into reagent bottle and multiple pipes on connecting convex peak, multiple first pipe holes are equipped on the convex peak, the inside of support is equipped with supporting plate, multiple second pipe holes are equipped on supporting plate, one end of pipe is connected first pipe hole, and the other end is connected titration tube through second pipe hole, color sensor is equipped above each titration tube, conical flask is equipped below each titration tube, magnetic stirrer is equipped below each conical flask, device supports multichannel synchronous operation, end point color can be calibrated storage, solve artificial titration end point determination error and fatigue problem, significantly improve the accuracy and efficiency of batch detection.

The embodiment of the invention discloses a tRNA (transfer ribonucleic acid) site specific fragmentation-sequencing method based on gamma-toxin, and relates to the technical field of molecular biology. The technical problem that in the prior art, the recognition accuracy of a modification site of tRNA is low is solved. The tRNA site specific fragmentation-sequencing method based on gamma-toxin comprises the following steps: constructing to obtain a recombinant gamma-toxin protein, and purifying the recombinant gamma-toxin protein to obtain an intermediate product; carrying out a gamma-CT-seq process on the intermediate product; the gamma-CT-seq process comprises the following steps: enriching and demethylating small RNA (Ribonucleic Acid), demethylating AlkB in vitro, specifically cutting a gamma-toxin site, and constructing a library and sequencing.

The present application belongs to the field of precision measurement, and particularly discloses a method for measuring the transverse temperature of an atomic group in an atomic interferometer. The present application first controls the size of the atomic group at the moment of acting on the Raman light pulse by adjusting the opening time, and then performs a Rabi oscillation experiment under different atomic group sizes. Then, the experimental data are fitted with a trigonometric function, and the key parameters reflecting the transverse temperature are extracted therefrom. Finally, the transverse temperature of the atomic group is obtained by inversion calculation. The method is based on the Gaussian distribution of the atomic group density and the Raman light intensity in the transverse space, and the coupling result of the two is the non-uniformity of atomic transition in the transverse space, thereby affecting the average transition probability of the whole atomic group, i.e. the linearity of the Rabi oscillation curve. Conversely, the linearity of the Rabi oscillation of the atomic group contains the transverse parameters of the atomic group, and the transverse parameters of the atomic group are extracted from the linearity of the Rabi oscillation. The present application can be completed only by relying on the atomic interferometer itself, and has low requirements for equipment, simple process and easy operation.

The invention provides a ligand surface preparation implementation method and device, electronic equipment and a storage medium. The SPR equipment comprises a plurality of channels; the method comprises the following steps: detecting binding information of a ligand solution corresponding to each channel; on the basis of binding information, detected by the SPR equipment, of the ligand solution corresponding to each channel, sample introduction parameters of the ligand solution in each channel are adjusted respectively, so that the ligand binding amount of each channel is controlled.

The application discloses a universal programmable segmented linear function simulation circuit and relates to the technical field of electronic information engineering, comprising a judgment module, a selection module and an addition module; the judgment module comprises multiple judgment branches, each of which comprises a comparator and a threshold voltage, the noninverting input end of the comparator is connected to an original input signal, and the inverting input end is grounded through the threshold voltage; the selection module comprises a controllable bias voltage and analog switches equal in number to the judgment branches, the control ends of the analog switches are connected to the output ends of the comparators one by one, and the input ends of all the analog switches are grounded through the controllable bias voltage; the addition module comprises an inverting adder and an input resistor, the input end of the inverting adder is connected to the output ends of all the analog switches and is connected to the original input signal through the input resistor, and the output end of the inverting adder serves as a circuit output. The application simulates a segmented linear function and has the characteristics of universality, online programmability and easy expansion.

This invention relates to the fields of genetic engineering and plant biotechnology, specifically to a method for single expression cassette knockout and overexpression of multiple genes. This method constructs a single expression cassette comprising a constitutive promoter, a Cas9 gene, a linker CAATCAAAC, multiple overexpressed genes, multiple sgRNA expression cassettes, and a terminator. Plants are transformed using Agrobacterium-mediated transformation, achieving simultaneous knockout of multiple endogenous gene sites and overexpression of multiple exogenous genes in a single genetic transformation. The linker connects the Cas9 gene to the overexpressed genes and among the overexpressed genes themselves. This invention offers advantages such as ease of operation, high efficiency, short cycle time, and the ability to simultaneously regulate complex metabolic pathways, making it of significant application value in crop genetic engineering and metabolic breeding.

This invention relates to the field of marine clean energy simulation technology, and discloses a rapid indoor simulation non-destructive evaluation method for hydrate-containing sediments, comprising the following steps: S1, preparing an indoor simulation system for simulation: the indoor simulation system includes a sample saturation component, a sample component, an acoustic testing component, a temperature control component, and a data acquisition component; S2, preparing and saturating the sample. This invention can rapidly generate hydrates and directly prepare saturated sediment samples under indoor conditions, significantly shortening the sample preparation cycle; it avoids hydrate decomposition or structural disturbance during saturation, improving sample structural stability and experimental repeatability; the sample remains within the ring cutter throughout the preparation and testing process, eliminating the need for demolding and significantly simplifying the experimental procedure; it achieves non-destructive evaluation of hydrate saturation through acoustic testing, and can combine temperature monitoring to reflect the internal phase transition process and latent heat effect of the sample in real time.

The application discloses a method for inducing the mycelial phase of Leucomycicella bicuspidata based on carbon quantum dots and application thereof, and the method comprises the following steps: preparing carbon quantum dots by a hydrothermal method, inducing culture of the mycelial phase, and morphological observation and confirmation. The carbon quantum dots are prepared by hydrothermal reaction of citric acid and urea at 230 DEG C for 15 hours, and the particle size is 2-8 nm; the carbon quantum dots are added into a Sabouraud glucose culture medium, the final concentration is 0.5 mg / mL, and the Leucomycicella bicuspidata is cultured at 28 DEG C for 24-28 hours, so that the mycelial phase of the Leucomycicella bicuspidata can be significantly induced; in combination with microscopic observation and PCR molecular confirmation, the mycelial formation rate can reach more than 85%. The method provides stable and reliable experimental materials for the study of the pathogenic mechanism and the prevention and control technology of the Leucomycicella bicuspidata.