32results about How to "Simplify the experimental process" patented technology

The invention discloses a carbon ceramic composite conductive microsphere, a ceramic resistor and a preparation method, and belongs to the technical field of production of special materials of electrical equipment, and the method comprises the following steps: sequentially dipping porous mullite in a precursor solution of carbon and slurry containing carbon black, and carrying out drying treatment and carbon burying sintering to obtain the carbon ceramic composite conductive microsphere; the preparation method comprises the following steps: preparing a ceramic raw material, performing vacuum-assisted centrifugal impregnation on the ceramic raw material and slurry consisting of a binder in pug consisting of the ceramic raw material and a surfactant, sequentially performing drying and glue discharging treatment, primary isostatic pressing forming and air pressure sintering, cooling to room temperature, and performing mechanical treatment and electrode spraying to obtain the ceramic resistor. Impregnation improves the dispersibility and uniformity of the carbon material in the porous mullite to form a continuous conductive path, the carbon black-containing slurry improves the conductivity of the ceramic resistor, carbon-buried sintering generates in-situ carbon with abundant types, the combination of carbon and a ceramic interface is promoted, and the oxidation resistance, mechanical properties and electrical properties of the ceramic resistor are improved.

The invention discloses a lithium ion battery DC internal resistance-based battery health state prediction method and system, and relates to the technical field of battery health state prediction. The method comprises the following steps: collecting pulse voltage data of a whole life cycle of a single lithium ion battery, and preprocessing the pulse voltage data; extracting a battery voltage rebound characteristic curve according to the preprocessed pulse voltage data; according to the extracted battery voltage rebound characteristic curve, the current battery health state is evaluated, and an evaluation result is output; and predicting the battery life according to the current battery health state evaluation result, and outputting a prediction result. According to the method and the system, the process of predicting the health state of the battery can be effectively simplified in the process of predicting the health state of the battery, the method and the system can be widely applied to actual situations, and the precision and the efficiency of predicting the health state of the battery are effectively improved; and internal aging and safety conditions of the battery are effectively fed back, so that an abnormal battery is timely replaced and handled.

The utility model relates to the technical field of liquid chromatographs, in particular to a liquid chromatogram sampling device which comprises a base, supporting legs are fixedly mounted at four corners of the top of the base, a top seat is fixedly mounted at the tops of the supporting legs, and a sample storage mechanism is fixedly mounted at the top of the top seat. By twisting the adjusting handle and pushing the guide block to rotate, the adjusting plate moves outwards, then the adjusting frame and the connecting shaft are pushed to move outwards, the limiting block is contracted, the limiting frame is separated, and the injector can be pulled out, meanwhile, the elastic clamping frame can stably clamp the piston rod, dismounting is convenient, and during mounting, a new injector is inserted into the limiting frame, the adjusting handle is loosened, and the piston rod can be pulled out. The limiting spring is reset, the limiting block is pushed to move inwards, the injector is clamped and positioned, then the top of the piston rod is clamped through the elastic clamping frame, and installation is completed, so that the operation process is simplified, the use convenience is improved, the injector can be rapidly replaced, and the device operation is further simplified.

The invention discloses a light-activated chemiluminescence immunodetection method for rapidly quantifying C-reactive protein (CRP), and belongs to the technical field of marker detection. Comprising a luminous microsphere-CRP coated antibody complex, a biotin-CRP labeled antibody complex and a streptavidin coated photosensitive microsphere. The light-sensitive microspheres and the light-emitting microspheres are ingeniously combined, and key factors such as coupling efficiency of antibodies on the surfaces of the light-emitting microspheres, reagent dosage, incubation temperature and time and the like are elaborately optimized, so that an optimized light-activated chemiluminescence detection system is constructed. The system not only simplifies the experimental process, but also ensures the reliability and consistency of the detection result by enhancing the stability and specificity of signal transduction. By screening and optimizing conditions of the system, the invention successfully develops a novel CRP (C-reactive protein) light-activated chemiluminescence immunoassay kit, and the detection limit is 6.20 ng.mL <-1 >.

The invention discloses a space-time transcriptome method which comprises the following steps: step 1, constructing a space transcriptome sequencing chip which is provided with a capture probe with space coordinates; step 2, carrying out metabolic marking by adopting a nucleotide analogue, freezing and embedding a slice after marking is completed, pasting the cut tissue slice on a sequencing chip, and carrying out fixed permeabilization so as to enable cells to release mRNA to be captured in situ by a capture probe on the space transcriptome sequencing chip; 3, carrying out treatment by using a nucleoside conversion reagent to cause base mutation in the corresponding cDNA, so as to distinguish the new RNA from the original RNA; and 4, analyzing the dynamic change of RNA under different external stimulation time.

The invention relates to the technical field of biology, in particular to a DNA tag library construction method based on droplet sorting and a magnetic microsphere technology, according to the method, the concentration of DNA tag template sequences is adjusted through Poisson distribution, it is ensured that each droplet contains at most one DNA tag template sequence, the problem that multiple templates are mixed into the droplets can be solved, and the DNA tag library construction efficiency is improved. The method has the advantages that the method is simple and convenient to operate, label repetition and collision are avoided, uniqueness of DNA labels in liquid drops is guaranteed, DNA label sequences after PCR amplification are further captured through magnetic microspheres, it is guaranteed that the amplified DNA labels can be efficiently recycled and purified, interference of non-specific reaction in PCR amplification is avoided, and the method is suitable for large-scale popularization and application. The combination of the technical points ensures the application advantages of the technical scheme in high-throughput sequencing, including diversity, accuracy and high efficiency of tags.

The application provides a construction of a fluorescent protein labeled microtubulin and a microtubule binding protein universal bivalent carrier, and the process is as follows: a first stage is to construct a GFP-alpha tubulin carrier, and a second stage is to construct a GFP-alpha tubulin-mCherry universal bivalent co-expression carrier; in the second stage, 35S, mCherry and NOS sequences are inserted into a KpnI enzyme cutting site of a multiple cloning site of the GFP-alpha tubulin carrier constructed in the first stage, and a single nucleic acid enzyme cutting site is inserted into the 5' end and the 3' end of the mCherry sequence, respectively, and the single nucleic acid enzyme cutting site is XbaI, KpnI / Acc65I and AscI sequences, respectively, so as to obtain the universal bivalent carrier. The universal bivalent carrier provided by the application can express two target genes simultaneously, has the fluorescent signals of GFP and mCherry, can quickly and accurately identify a transgenic plant, is convenient for positive seedling screening, can be used for a tobacco transient expression experiment, can shorten an experimental period, and saves time and effort.

The invention belongs to the technical field of battery materials, discloses a method for auxiliary recovery of a waste lithium battery negative electrode material by using a transition metal burning reducer, and designs a method for auxiliary recovery of a waste lithium battery negative electrode material by using a transition metal burning reducer. Transition metal salt ferrous sulfide and acid-leached graphite are mixed and subjected to heat treatment after the acid leaching process, the reaction activity of the graphite material is enhanced through the ferrous sulfide, and therefore the temperature needed by recovery of a waste graphite heat treatment crystal structure is reduced. The selected transition metal salt ferrous sulfide is low in cost. On the basis of the existing research, the sintering temperature is further reduced to 750 DEG C by adopting ferrous sulfide, and the regenerated graphite has a good crystal structure. The filtrate without sulfur and iron elements can be reused to regenerate iron metal and can also be reused to reduce burning. The whole experiment process is relatively simple, and the experiment repeatability is high.

This invention discloses a sequencing technique for enriching mutations after library construction. The co-priming amplification (Co-PCR) technique of this invention couples a mutation probe to a short P5 / P7 primer. This short primer has a low melting temperature (Tm) and cannot hybridize and amplify on its own at the reaction temperature. Only when the mutation probe hybridizes to the mutation site can it assist the coupled short P5 / P7 primer in hybridizing to the adjacent P5 / P7 sequence at the end of the pre-library, initiating amplification. Therefore, this Co-PCR technique not only amplifies a specific mutation in a specified gene but also amplifies from both ends of the original pre-library, replicating the complete sequence information of the pre-library, including sequencing adapters, indexes, and sequencing primers. The amplified products can be directly sequenced after purification. By optimizing the co-primers, Co-PCR enrichment amplification of five sites in the pre-library was achieved, resulting in nearly 100-fold enrichment.

The invention discloses an RNA (Ribonucleic Acid) target region enrichment method based on a circular probe, and belongs to the technical field of biomolecule detection and gene sequencing. The method comprises the following steps: designing an annular probe containing an anchoring sequence, a UMI sequence and a universal primer sequence (at least one anchoring sequence 5'end is phosphorylated and modified); the method comprises the following steps: hybridizing a probe with target RNA (Ribonucleic Acid), enriching a hybridization compound through oligo d (T) magnetic beads, cyclizing the probe through DNA ligase, and carrying out PCR (Polymerase Chain Reaction) amplification to obtain an enriched product. Reverse transcription and extension steps are not needed, a solid-phase substrate is not needed, the experimental process is simplified, and the operation time is shortened; through combination of specific probe design and magnetic bead enrichment, high-selectivity enrichment of target RNA is realized, the method is especially suitable for differential expression analysis of samples with low expression quantity, and the template recovery rate and the effective data proportion are effectively improved.

The invention provides a single-point light-operated DNA synthesis device which comprises a light path module, the light path module comprises at least one synthesis starting light source, a collimation and convergence lens group of the synthesis starting light source and a synthesis flow cell, and the collimation and convergence lens group is arranged to collimate and converge light emitted by the synthesis starting light source to the synthesis flow cell. And forming a light spot of a single DNA synthesis site. The single-point light-operated DNA synthesis device disclosed by the invention adopts a structural scheme that only one DNA synthesis site is arranged, so that the single-point light-operated DNA synthesis device is suitable for a research and development process, and various indexes of photosensitive molecules in a chemical reaction are obtained during a whole-process test; due to the fact that only one synthesis site is adopted, various indexes are reduced, the complexity of an instrument can be reduced, the cost of equipment can be reduced, the experimental process can be simplified, and various indexes and performance of photochemical synthesis DNA can be rapidly, efficiently and accurately obtained at low cost.

The invention provides an ion transmembrane hydration number testing method based on non-equilibrium thermodynamics. The testing method comprises the following steps: clamping a membrane in an H-type electrolytic tank, and respectively filling different solutions at two sides of the membrane, so as to measure the potential difference at two sides of the membrane. Specifically, the electrolytic tank on one side is filled with a mixed solution containing salt and non-membrane neutral molecules, the electrolytic tank on the other side is only filled with a salt solution, and the salt concentrations on the two sides are the same. The potential difference between the two sides of the membrane is tested through an electrochemical workstation, mathematical fitting is conducted according to the change of the potential difference along with time, and therefore the relation between the potential difference and the ion transmembrane hydration number is obtained, and finally measurement of the ion transmembrane hydration number is achieved. The method can quantify the transmembrane hydration state of the ions, and is of great significance to research on an ion mass transfer mechanism, selective separation of the ions, physicochemical characteristics of the membrane and the like.

The invention discloses a reconstruction method and a reconstruction device of a space protein expression map and a storage medium. Comprising the following steps: receiving a multi-channel high-resolution tissue image of a tissue region obtained based on tissue slices; receiving a polymerization true value of the expression quantity of each target protein on each first-direction tissue band and each second-direction tissue band, wherein the polymerization true value is obtained after the first-direction tissue band and the second-direction tissue band are cut on the first tissue slice and the second tissue slice; a deep learning network is trained based on the aggregation true values of the protein expression quantities on the tissue strips in the first direction and the second direction and the multi-channel high-resolution tissue image, and the trained deep learning network predicts the expression quantity of each target protein on each image unit based on the multi-channel high-resolution tissue image; and reconstructing a two-dimensional protein expression map. According to the method, the two-dimensional space protein map can be reconstructed with high precision only by using as few as two tissue slices and the aggregation value of the protein expression quantity on the tissue strip which is relatively easy to obtain without the protein expression quantity of each unit.

The utility model belongs to the field of atom inertia measurement, and particularly discloses an auxiliary device for evaluating the Coriolis force of an atom interferometer, the auxiliary device comprises a lifting rotation supporting module and an inclination detection adjusting module, and the inclination detection adjusting module comprises an inclination detection unit and an inclination adjusting unit; the inclination detection unit is used for detecting the inclination of the vacuum container; the inclination adjusting unit is located at the bottom of the vacuum container and used for supporting the vacuum container and adjusting the vacuum container to be horizontal according to the inclination. The lifting rotation supporting module can be coaxially arranged under a vacuum container of the atom interferometer and can drive the vacuum container to rotate so as to modulate the Coriolis force. According to the invention, the effect of rotating the vacuum container in the atom interferometer with high efficiency and high precision can be realized.

The utility model discloses a multi-slit interference device based on spatial light phase modulation. The device belongs to the field of diffraction light field regulation and control and comprises a light source, a collimating lens, a first spectroscope, a phase-type spatial light modulator, a first projection system lens, a diaphragm, a second projection system lens, a digital micromirror, a second spectroscope, a camera and the like. According to the utility model, the spatial light modulator and the digital micromirror are controlled through software to modulate the phases and the shapes of the multiple slits respectively, and any phase type multiple slits are constructed; according to the phase-type multi-slit diffraction and interference theory provided by the utility model, the device can flexibly construct multiple slits and control the spatial form of interference fringes so as to generate a target light field with multiple purposes; the experimental device has the characteristics of modularization, small occupied volume and low assembly difficulty.

The present invention relates to a soybean seed vitality evaluation method based on multi-index comprehensive evaluation, which belongs to the field of plant production technology. The artificial accelerated aging method was used to measure 11 seed vitality-related indicators of 126 soybean local varieties screened from a germplasm bank containing 23,587 soybean resources. Through principal component analysis, a soybean seed vitality comprehensive evaluation system was established, and on this basis, a concise and efficient seed vitality evaluation model was constructed: V=‑ 0.026+0.625×RSL+0.485×RGI. In this model, relative seedling length (RSL) and relative germination index (RGI) have a significant positive effect on seed vigor. The model provides excellent vigor assessment performance and 87.30% vigor classification accuracy. The present invention provides a new method for the scientific evaluation of soybean seed vigor, which can effectively evaluate the vigor of soybean seeds before sowing and monitor the changes in soybean seed vigor, thereby improving soybean planting efficiency and yield, and at the same time provides a valuable method for seed quality control and screening.

The invention provides a development method and application of a novel molecular marker, and belongs to the technical field of molecular markers. The invention provides a molecular marker development method, according to a significant variation site provided by a GWAS result, a common primer is designed near the significant variation site, a new variation site is found through Sanger sequencing, and genetic typing, correlation analysis and the like are carried out by utilizing a molecular biological technology for verification, so that a brand new molecular marker is obtained. According to the method, the number of variation sites is large, the requirement for experimental equipment is relatively low, the experimental process is relatively simple, result guiding is more accurate, purposiveness is higher, and developed molecular markers are also remarkable. The brand new molecular marker screened by the method provided by the invention provides an experimental basis for genetic breeding of HuaMongolian mutton sheep, and provides a new idea for accelerating breeding of new sheep varieties.

The utility model relates to chemical analysis instrument technical field, concretely is a kind of automatic titration device, including support, reagent bottle is equipped with inverted installation on support, the convex peak that can be inserted into reagent bottle and multiple pipes on connecting convex peak, multiple first pipe holes are equipped on the convex peak, the inside of support is equipped with supporting plate, multiple second pipe holes are equipped on supporting plate, one end of pipe is connected first pipe hole, and the other end is connected titration tube through second pipe hole, color sensor is equipped above each titration tube, conical flask is equipped below each titration tube, magnetic stirrer is equipped below each conical flask, device supports multichannel synchronous operation, end point color can be calibrated storage, solve artificial titration end point determination error and fatigue problem, significantly improve the accuracy and efficiency of batch detection.

The invention discloses a dew point temperature measuring method and system for mixed gas containing perfluoroisobutyronitrile, and relates to the technical field of power switchs.The method comprises the steps that a pressure container is vacuumized, and the perfluoroisobutyronitrile mixed gas in a specific proportion is inflated through an inflation device; measuring initial pressure and temperature by using an air pressure sensor and a temperature sensor to obtain an initial condition pressure-temperature ratio; the temperature of the temperature control box is adjusted, and the real-time pressure-temperature ratio is obtained; continuously reducing the temperature until the real-time pressure-temperature ratio is smaller than the initial condition to obtain the dew-point temperature; according to the invention, accurate temperature control and real operation condition simulation are facilitated, and the measurement accuracy is ensured; the reliability of a measurement result is improved; the experiment process is simplified, and the efficiency is improved; the dew-point temperature can be measured without a gas chromatograph, the cost and the operation difficulty are reduced, the dew-point air pressure change in the operation process of the power equipment is simulated, and the evaluation of the actual application performance is facilitated.

The embodiment of the invention discloses a tRNA (transfer ribonucleic acid) site specific fragmentation-sequencing method based on gamma-toxin, and relates to the technical field of molecular biology. The technical problem that in the prior art, the recognition accuracy of a modification site of tRNA is low is solved. The tRNA site specific fragmentation-sequencing method based on gamma-toxin comprises the following steps: constructing to obtain a recombinant gamma-toxin protein, and purifying the recombinant gamma-toxin protein to obtain an intermediate product; carrying out a gamma-CT-seq process on the intermediate product; the gamma-CT-seq process comprises the following steps: enriching and demethylating small RNA (Ribonucleic Acid), demethylating AlkB in vitro, specifically cutting a gamma-toxin site, and constructing a library and sequencing.

The present application belongs to the field of precision measurement, and particularly discloses a method for measuring the transverse temperature of an atomic group in an atomic interferometer. The present application first controls the size of the atomic group at the moment of acting on the Raman light pulse by adjusting the opening time, and then performs a Rabi oscillation experiment under different atomic group sizes. Then, the experimental data are fitted with a trigonometric function, and the key parameters reflecting the transverse temperature are extracted therefrom. Finally, the transverse temperature of the atomic group is obtained by inversion calculation. The method is based on the Gaussian distribution of the atomic group density and the Raman light intensity in the transverse space, and the coupling result of the two is the non-uniformity of atomic transition in the transverse space, thereby affecting the average transition probability of the whole atomic group, i.e. the linearity of the Rabi oscillation curve. Conversely, the linearity of the Rabi oscillation of the atomic group contains the transverse parameters of the atomic group, and the transverse parameters of the atomic group are extracted from the linearity of the Rabi oscillation. The present application can be completed only by relying on the atomic interferometer itself, and has low requirements for equipment, simple process and easy operation.

A rigorous conditional expression promoter P535 is obtained through data analysis of a transcriptome and the like of vibrio natriureticus, Cas9 protein is expressed by using the promoter, a pMMB-TFC plasmid is constructed by integrating a naturally transformed gene tfox and a Cas9 encoding gene (P535 promoter), a p15A plasmid containing targeted sgRNA and a homologous arm is constructed, and the rigorous promoter is obtained. And efficient natural transformation and gene traceless editing are realized. According to the method, the gene editing efficiency is improved, and the experimental process is simplified.

The invention provides a ligand surface preparation implementation method and device, electronic equipment and a storage medium. The SPR equipment comprises a plurality of channels; the method comprises the following steps: detecting binding information of a ligand solution corresponding to each channel; on the basis of binding information, detected by the SPR equipment, of the ligand solution corresponding to each channel, sample introduction parameters of the ligand solution in each channel are adjusted respectively, so that the ligand binding amount of each channel is controlled.

The invention relates to the technical field of high-throughput sequencing and library building, and discloses a DAP-seq sequencing and library building method, which comprises the following steps: firstly, designing specific primers which can be accurately identified and combined to a target gene sequence; then, the desired gene segments are amplified through the PCR technology, purification is conducted, and it is ensured that high-purity target DNA is obtained to serve as a gene template for in-vitro protein translation; in an in-vitro protein expression stage, a specific expression system is utilized, and a template is input to synthesize a target protein. The proteins are subsequently combined with specific DNA in the screening process of DNA fragments, and the DNA fragments are screened out through an affinity purification technology by utilizing the interaction between the proteins and the DNA. The DNA fragments are constructed into a library for computer sequencing. The operations can accurately identify, extract and utilize the target DNA fragment, and a new way is opened up for exploring the combination of the transcription factor and DNA.

The invention belongs to the field of toxin protein recognition, and particularly relates to an aquatic echinocystis toxin protein recognition method, system, terminal and medium, a toxin protein recognition model is constructed, and the toxin protein recognition model comprises a feature coding module and a classifier module. The feature coding module performs toxin protein coding by using amino acid composition, a word2vec technology and normalized Moreau-Broto self-correlation description to obtain coding features, and the classifier module classifies the coding features to identify the aquatic echinocystis toxin protein; training the constructed toxin protein recognition model; and identifying the target toxin protein by using the trained toxin protein identification model so as to judge whether the target toxin protein is the aquatic echinocystis toxin protein or not. According to the method, the toxin protein of the aquatic echinocystis is identified through the model based on the random forest, the efficiency and accuracy of identifying the toxin protein of the aquatic echinocystis are effectively improved, and the cost is relatively low.

The invention relates to equipment and a method for monitoring physiological indexes of a water-soluble fertilizer. A light-transmitting plate covered with capillary action media such as non-woven fabric, filter paper or Chinese art paper serves as a monitoring plate, a water-soluble fertilizer solution is evenly distributed on the monitoring plate through the capillary action, the permeation and distribution states of the fertilizer in soil are simulated, and the influence of the fertilizer solution on seeds such as the growth speed and the germination rate is monitored in real time. Therefore, the physiological indexes of the water-soluble fertilizer can be accurately evaluated. According to the overall design of the equipment, the testing process of the physiological indexes of the water-soluble fertilizer is simpler and more efficient, errors caused by manual operation are reduced, the testing efficiency and accuracy are improved, the physiological indexes of the water-soluble fertilizer can be tested repeatedly, the reliability and comparability of testing results are improved, and the testing cost is reduced. More scientific and reasonable fertilizer use suggestions can be provided for agricultural production, and the yield and quality of crops are improved.

The utility model relates to the technical field of extraction of organic selenium in food, in particular to a vacuum filtration and purification device for extraction of organic selenium in food, and adopts the technical scheme that the vacuum filtration and purification device comprises an air pump body, a storage box and a purification bin, the air pump body is fixedly mounted on the front surface of the storage box, the purification bin comprises a bottom bin, and a top bin is movably mounted on the upper surface of the bottom bin; a hole is formed in the top of the inner side of the top bin and is communicated with a communicating pipe, a sampling pipe is movably inserted into the communicating pipe, a placing frame is movably installed in the bottom bin, a material receiving pipe is movably inserted into the placing frame and is located under the sampling pipe, and a hole is formed in the rear end of the bottom bin and is communicated with the sucking pump body through a guide pipe. According to the invention, modernization and high efficiency of an experimental sample preparation process are realized, a reliable and efficient operation platform is provided for the experimental process, meanwhile, the whole preparation process is more active and efficient, and the common problems of slow dripping, filtering blockage and the like in gravity filtration are avoided.

The application discloses a universal programmable segmented linear function simulation circuit and relates to the technical field of electronic information engineering, comprising a judgment module, a selection module and an addition module; the judgment module comprises multiple judgment branches, each of which comprises a comparator and a threshold voltage, the noninverting input end of the comparator is connected to an original input signal, and the inverting input end is grounded through the threshold voltage; the selection module comprises a controllable bias voltage and analog switches equal in number to the judgment branches, the control ends of the analog switches are connected to the output ends of the comparators one by one, and the input ends of all the analog switches are grounded through the controllable bias voltage; the addition module comprises an inverting adder and an input resistor, the input end of the inverting adder is connected to the output ends of all the analog switches and is connected to the original input signal through the input resistor, and the output end of the inverting adder serves as a circuit output. The application simulates a segmented linear function and has the characteristics of universality, online programmability and easy expansion.

This invention relates to the fields of genetic engineering and plant biotechnology, specifically to a method for single expression cassette knockout and overexpression of multiple genes. This method constructs a single expression cassette comprising a constitutive promoter, a Cas9 gene, a linker CAATCAAAC, multiple overexpressed genes, multiple sgRNA expression cassettes, and a terminator. Plants are transformed using Agrobacterium-mediated transformation, achieving simultaneous knockout of multiple endogenous gene sites and overexpression of multiple exogenous genes in a single genetic transformation. The linker connects the Cas9 gene to the overexpressed genes and among the overexpressed genes themselves. This invention offers advantages such as ease of operation, high efficiency, short cycle time, and the ability to simultaneously regulate complex metabolic pathways, making it of significant application value in crop genetic engineering and metabolic breeding.

The invention relates to a CNCs modification method. The CNCs modification method comprises the following steps: S1, preparing a CNCs aqueous solution; and S2, GMA is added into the CNCs aqueous solution for chemical modification, hydroxyl groups of the CNCs and epoxy groups of the GMA are subjected to a ring-opening reaction, so that the GMA is grafted to the surfaces of the CNCs to form a CNCs-GMA aqueous solution, and carbon-carbon double bonds of the GMA are reserved and introduced into a CNCs-GMA structure. According to the CNCs modification method, a more advanced CNCs stimulation response system can be obtained, the stability of the material can be guaranteed, additional chemical properties can be given, the method can be used for preparing a CNCs photonic crystal sensor, the detection range of CNCs is widened, new possibility and thought are provided for CNCs in the field of molecular detection, and then the application field of CNCs is expanded.