10 results about "New mutation" patented technology

Provided are a method and apparatus for recommending a protein mutation, and a computer device and a storage medium. The method comprises: receiving a mutation task request, wherein the request carries a specified first mutation instruction; in response to the request, generating third mutation instructions on the basis of the first mutation instruction and second mutation instructions; mutating an original protein sequence, so as to obtain mutated protein sequences corresponding to the third mutation instructions on a one-to-one basis; then, displaying scoring results and the third mutation instructions in a manner of corresponding to each other on a one-to-one basis; and determining whether a new mutation task request has been received, and if so, returning to continue to receive the new mutation task request. It can be seen that after the scoring results and the third mutation instructions are displayed in a manner of corresponding to each other on a one-to-one basis and a user performs experimental verification, a first mutation instruction is specified in a mutation task request again, thus forming a cyclic operation until the user finds an optimal protein sequence. Therefore, the user does not need to manually input a protein sequence, and thus the operation is convenient, and the efficiency of mutated-protein screening can be improved.

The present invention relates to a subtilisin BPN' variant or homologue thereof comprising new mutations compared to subtilisin BPN' as shown in SEQ ID NO: 2 or a homologous sequence thereof. Such mutations can occur at amino acid positions selected from the group consisting of L96, D99, A223 and S224. The present invention also relates to a method for the enzymatic synthesis of a peptide by coupling of peptide fragments, wherein said coupling is catalysed by said subtilisin BPN' variant or homologue thereof.

The application discloses a test case automatic generation method and system based on MoMuTUML, reduces and deletes original function overlapping mutation operators, and adds three new mutation operators which are not functionally overlapped with the original mutation operators, so that the generation of a syntax equivalent mutation model is reduced, and the test case generation time cost is reduced; test cases are generated according to a mutation model set and a model under test, and equivalent mutation models are deleted according to the test cases, so that effective mutation models are obtained, the problem of too many equivalent mutation models in MoMuTUML is avoided, and the test case generation efficiency is improved; through a feedback-based mutation method, the mutation operators corresponding to the effective mutation models are scored, an optimal mutation operator set is constructed, and the mutation operators in the set are selected to perform mutation operations on the model under test, so that the problem of low efficiency of the MoMuTUML mutation method is solved, and the test case generation efficiency is further improved.

The invention provides a novel base editing system for mediating multi-base saturation mutation. Specifically, the present invention provides a multi-base editor comprising one or more elements selected from the group consisting of: (a) a nuclear localization signal; (b) a cytosine deaminase; (c) adenine deaminase; (d) a Cas protein or a variant thereof or an active fragment thereof; (e) an alkyl adenine DNA glycosylase or a variant thereof; (f) a linker; and optionally (g) an N-methylpurine DNA glycosylase protein. The invention also provides a multi-base editing system based on the multi-base editor, a corresponding polynucleotide, a vector, a host cell and application thereof. According to the multi-base editor and the editing system disclosed by the invention, saturation mutation of two bases and three bases (mediating Agt; c / T / G and / or Cgt; t / G / A and / or Ggt; c / T / A), in particular, a new mutation type Agt is generated; c / Tamp; cgt; t / G / A and Agt; c / T / Gamp; cgt; t / G / Aamp; ggt; the rich saturated mutation spectrum of C / T / A can greatly promote gene function screening, protein evolution, pedigree tracing and other applications.

The invention discloses a probe set for evaluating whole-body tumor load of small cell lung cancer and application of the probe set, the probe set comprises a plurality of oligonucleotide probes for specifically capturing mutation regions of SCLC related genes in a targeted mode, and the genes are selected from TP53, RB1, CREBBP, EGFR, PTEN, PIK3CA, LRP1B and NOTCH1; and the probe set is capable of covering at least 94.9% of at least one mutation existing in an SCLC patient sample. The invention relates to the technical field of biotechnology and molecular diagnosis, and has the beneficial effects that based on a clinical queue, the probe group incorporates high-frequency mutant genes of SCLC, can be superposed with personalized probes for use, and is used for ctDNA detection of SCLC patients and evaluation of whole-body tumor load. The SCLC cancer species specific probe group provided by the invention plays a role in monitoring tumor evolution and new mutation, can overcome the space-time heterogeneity of tumors to a certain extent, and also can improve the capture efficiency at the same time.

The application belongs to the technical field of gene detection, and particularly relates to a construction method and kit of a gene library for detecting new mutations of dominant monogenic diseases before embryo implantation. The application provides a construction method of a gene library for detecting new mutations of dominant monogenic diseases before embryo implantation, which comprises the step of amplifying the gene of the new mutation of dominant monogenic diseases before embryo implantation and related copy number variation fragments in the genomic DNA of specific screening. The application precisely applies NGS sequencing technology to the screening of dominant monogenic diseases in trace cell samples, breaks through the technical bottleneck of high-throughput sequencing under extremely low initial DNA amount in the past, and realizes the screening of new mutations before embryo implantation, thereby providing a brand-new key technical path for blocking genetic birth defects at an earlier stage.

The application discloses a virus genome data analysis and prediction system based on deep learning, relates to the technical field of data management, and extracts local features of a genome and realizes classification and variation detection by using a convolutional neural network through a deep learning model module; a robustness evaluation module quantifies the performance fluctuation of the model in new mutations by analyzing a new mutation test data set; a confidence analysis module evaluates the abnormality of the model prediction confidence distribution; a comprehensive analysis module generates a comprehensive stability score in combination with the robustness and confidence results, dynamically quantifies the stability of the model to new mutations; and a model optimization module iteratively updates the model architecture and training data set based on the analysis results, thereby significantly improving the prediction performance of the model on dynamic variation of viruses, effectively solving the problem that existing models are difficult to adapt to the cumulative drift of virus genome mutations, providing an efficient and stable solution for rapid detection of new mutations, and having important public health significance.

This invention discloses a BTD gene knockdown hepatocyte injury model and its construction method, relating to the fields of molecular biology and cell biology. The model is used to knock down the expression of the BTD gene in human hepatocytes to construct a hepatocyte injury model. Its sense strand nucleotide sequence is 5'-GCGAUUGGUCUCAAGCUAA(dT)(dT)-3', and its antisense strand nucleotide sequence is 5'-UUAGCUUGAGACCAAUCGC(dT)(dT)-3'. This invention designs specific siRNA sequences to directionally knock out the BTD gene in human hepatocytes, thereby observing and verifying whether it leads to abnormalities in liver injury indicators. It clarifies the direct causal relationship between BTD gene functional defects and hepatocyte injury, providing powerful experimental tools and data support for the gene diagnosis of unexplained liver diseases, the pathogenicity assessment of new BTD gene mutation sites, and the development of related drugs.

The application provides a method and device for recommending protein mutations, a computer device and a storage medium, comprising: receiving a mutation task request, the request carrying a specified first mutation instruction; in response to the request, generating a third mutation instruction based on the first mutation instruction and a second mutation instruction; mutating an original protein sequence to obtain a mutated protein sequence corresponding to each third mutation instruction; then displaying the score results and the third mutation instruction one by one; determining whether a new mutation task request is received; if yes, continue to receive the new mutation task request; it can be seen that the score results and the third mutation instruction are displayed one by one, and the first mutation instruction is specified in the mutation task request after the user performs experimental verification, thus forming a cyclic operation until the user finds the optimal protein sequence, so that the user does not need to manually input the protein sequence, the operation is convenient, and the screening efficiency of the mutated protein can be improved.

The application belongs to the technical field of molecular detection, and particularly relates to a detection reagent for detecting the resistance of a spider mite to abamectin based on a mutation site of a GluCl2 subunit and a PCR-RFLP detection method. GluCl Gene sequence analysis finds that there is a new mutation site T76S on the GluCl2 subunit, the mutation exists in multiple abamectin-resistant field populations, and the mutation frequency of the site is increased after abamectin treatment. In the process of identifying the above mutation site, it is found that there is a specific recognition site (T'CAG) of DdeI restriction endonuclease in the sequence, and thus an amplification primer is designed, and a PCR-RFLP method for detecting the T76S mutation site is established. The method is fast in enzyme digestion reaction, simple in operation, not easy to be contaminated, short in time cost, accurate in experimental results, and easier in judgment method.