1results about How to "Low mismatch rate" patented technology

This invention relates to the interdisciplinary field of molecular biology and bioinformatics, specifically to a method and system for designing multi-site mutation primers based on rule constraints and multi-objective optimization. The method includes the following steps: receiving the target DNA sequence and mutation site information; automatically grouping the mutation sites according to their position distribution and spacing in the DNA sequence; determining the corresponding primer design region by extending a fixed length upstream and downstream from the center position of each mutation group; generating multiple candidate primer sequences based on the primer design region using a sliding window method; constructing a multi-objective optimization scoring function based on annealing temperature, GC content, and primer length to comprehensively score and screen the candidate primers to obtain the optimal primer; optimizing the 3' end of the optimal primer to preferentially contain stable bases; and outputting the optimized primer sequence and related design parameters and explanation information, effectively solving the pain points of existing multi-site mutation primer designs.