123results about How to "Aids in diagnosis" patented technology

The invention belongs to the technical field of neoplastic disease diagnosis, and discloses a kit for early diagnosis of neoplastic disease, a method and applications of the kit. The kit includes a specific capturing and detecting system for a tumor exosome and a molecular beacon fluorescent detection system. The specific capturing and detecting system for the tumor exosome includes a solution ofa magnetic gold nanosphere composite modified by a tumor exosome specific ligand and adopting ferric ferrous oxide as a core, and a gold nanocage solution modified by the exosome specific ligand. Themolecular beacon fluorescent detection system includes a molecular beacon probe provided with a fluorescent group, fluorescent amplification substrate hybrid chains provided with fluorescent groups and a buffer liquid. The kit and the method combine tumor exosome specific capturing and analysis and a molecular beacon fluorescent detection method, and greatly increases accuracy and reliability of early diagnosis of tumor.

The invention belongs to the fields of gene engineering and oncology and discloses a serum / plasma miRNA marker associated with breast cancer and application thereof. The marker is a combination of miR-16, miR-25, miR-222 and miR-324-3p. The maker and primers thereof can be used for preparing a diagnosis kit and assisting early diagnosis of the breast cancer.

The invention provides a bladder cancer patient urine specific metabolite spectrum, an establishing method and an application, and especially provides a method for establishing a spectrum of specific metabolites in urine of bladder cancer patients, and a method for screening a related specific biomarker. Through metabonomics, especially metabonomics based on liquid chromatography-mass spectrometry combined technology, the invention establishes a bladder cancer patient urine specific metabolite spectrum. The invention provides a basis for early diagnosis of bladder cancer and bladder cancer diseases with different pathological stages, and also provides a spectrum of specific metabolites in urine of bladder cancer patients and a related specific biomarker. The method provided by the invention has the characteristics of noninvasiveness, convenience, and rapidness, can accurately reflect the difference of metabolite spectra of bladder cancer patients and normal people, and has high specificity.

The invention relates to a kit for a serum / plasma lncRNA marker related to stomach cancer. The serum / plasma lncRNA marker is combination of MALAT1 and HOTAIR, a primer is a primer for the MALAT1 and the HOTAIR, and the upstream primer sequence and the downstream primer sequence of the MALAT1 are SEQ No.1 and SEQ No.2; the upstream primer and the downstream primer of the HOTAIR are SEQ No.3 and SEQ No.4. By means of the kit, the defect that a kit only adopting one serum / plasma lncRNA as the tumor marker is low in sensitivity and specificity, so that the fault diagnosis rate and the rate of early gastric cancer diagnosis are greatly raised is overcome; by means of the kit, the sensitivity and specificity of disease diagnosis are enhanced, successful development of the lncRNA biological marker is beneficial to auxiliary diagnosis of stomach cancer and provides reference for research of biological markers of other diseases, lncRNA chip detection is adopted to obtain a serum / plasma lncRNA expression profile for disease specificity and abnormity expression, a qRT-PCR method is applied to conduct single verification, and dye method is adopted to conduct verification.

The invention belongs to the fields of genetic engineering and oncology, and discloses a blood serum / blood plasma micro ribonucleic acid (miRNA) marker relevant with pancreatic cancer and application thereof. The marker is formed by combining miR-451 and miR-490-3p. The marker and primers of the marker can be used for preparing a diagnostic kit and are used for the auxiliary diagnosis of pancreatic cancer.

Provided is a simulation test system of a hydraulic support electro-hydraulic control system. The simulation test system comprises a small hydraulic support model, a hydraulic support display board, a simulation device, and a PC server, the small hydraulic support is provided with a plurality of sensors and used for displaying the movement of a hydraulic support and attitude of each part of the support, the hydraulic support display board is used for displaying the movement of the hydraulic support and the states of main sensors in a graphic visual manner, the simulation device is electrically connected with the sensors and used for receiving data of the sensors and performing preliminary processing of the data, and the PC server is electrically connected with the simulation device and used for receiving the data after preliminary processing by the simulation device and performing post-processing of the data and downloading on-site system operation data to a test system and performing reproduction of the on-site operation data and simulation of the system operating environment. According to the simulation test system, the on-site system operation data is collected and downloaded to the test system and is mutually integrated with the simulated test system via a control system, and the laboratory simulation tests in the condition of on-site environment are realized.

The invention relates to molecular genetics, and provides a bovine serum microRNA molecular marker of a milk cow fatty liver disease and a milk cow perinatal period-associated metabolic disease. The molecular marker is used for early detection and diagnosis of a diseased cow in production practice of the milk cow, thus greatly improving diagnosis accuracy and greatly avoiding economic loss to the great extent. The marker can be used for variety breeding and idioplasm improvement of the milk cow.

The invention discloses a blood miRNA marker related to liver cancer and an application thereof. The marker is the combination of tumor-induced exosome sourced miRNA-26a and miRNA-199a in human blood. According to the invention, tumor-induced exosome in peripheral blood of liver cancer patient and liver benign disease patient can be separated and purified, miRNA of two expression difference can be analyzed and screened by a miRNA chip, and the above conclusion is passed by a QPCR verification. The marker and its detection reagent can be used for preparing a diagnostic kit, which can be used for early diagnosis of liver cancer.

A multi-parameter photoelectric human balance function test system belongs to the field of medical apparatus and instruments. Photoelectric technology and serial image analysis technology are adopted. The test system consists of a digital CCD camera (1), a USB cable (2), a printer (3), a mechanical bracket (4), a plurality of sighting targets (5.6.7), a computer (8) and a human balance analysis system software. The system synchronously records the wobbling regularity of a plurality of key parts of human body on a 2D plane to acquire an objective indicator describing human balance capacity and perform the balance function of a multi-joint in balance adjustment. The multi-parameter photoelectric human balance function test system overcomes the defect of the single test indicator of a current balance admeasuring apparatus, evaluates the balance state of a plurality of parts and provides more objective data for the diagnosis of diseases. The multi-parameter photoelectric human balance function test system has the advantages of simplicity, directviewing, low cost of a complete appliance, easy spread and exploitation, etc.

The invention belongs to the technical field of wind tunnel test apparatus, and specifically relates to a wind tunnel double light path schlieren flow field display apparatus. The wind tunnel double light path schlieren flow field display apparatus includes a fiber coupling LED color light source, a condenser set, a spectroscope, a planar mirror, an optical window, a wind tunnel test model, a spherical reflector, a wind tunnel test section, a cutter edge, an imaging object lens and a camera, wherein the wind tunnel test section is a vacuum sealed compartment; and the test model and the spherical reflector are successively arranged in the wind tunnel test section. The wind tunnel double light path schlieren flow field display apparatus has the advantages of being simple in structure, beinglow in cost, being preferable in the flow field display effect, and being convenient for operation. The wind tunnel double light path schlieren flow field display apparatus solves the flow field display problem which cannot be solved by a conventional schlieren and glow discharge apparatus for nearly continuous flow to rarefied transitional flow (the corresponding flow field static pressure is 100Pa-20Pa and the corresponding test Mach number is about M8-M12). The wind tunnel double light path schlieren flow field display apparatus also solves the problem which is not preferably solved for more than 40 years, that is, the double light path schlieren flow field display apparatus is easy to generate flow field image ghosting.

The invention provides a method and system for processing a medical image. The method comprises the following steps that the image is read and displayed, the image is zoomed and rotated, negative film effect processing is conducted on the image, the image is cut, the grey level of the image is transformed, the contrast ratio of the image is increased, the image is displayed in the mode of a histogram, the histogram of the image is equalized, noise of the image is eliminated, the edges of the image are detected, the image is smoothed, and the image is sharpened. The system comprises a central processing unit, an image collecting module, an image processing module, a display module and a storage module, wherein the image collecting module, the image processing module, the display module and the storage module are respectively connected with the central processing unit. According to the method and system for processing the medical image, the medical image is read in and displayed, so that a series of processing is conducted on the medical image, the image processing technologies are fully used for effectively improving the image quality, diagnosis and treatment which are relative to the state of an illness and conducted by a doctor are facilitated, and the phenomena of missed diagnosis and misdiagnosis are avoided.

The invention relates to an ERBB signal pathway mutation targeted sequencing method for the prognosis evaluation of gallbladder carcinoma and provides an application of an ERBB signal pathway mutation targeted sequencing reagent / kit for gallbladder carcinoma patients. In the invention, the whole-genome exon technology is adopted for performing mutation target sequencing of the ERBB signal pathway of a gallbladder carcinoma patient; and in combination with the pathological clinical data of the gallbladder carcinoma patient, the Kaplan-Meier survival curve analysis indicates that the lifetime of the patient after operation is closely related to the gene mutation of the ERBB signal pathway, and the multiple-factor analysis of Cox risk regression model indicates that the gene mutation of the ERBB signal pathway can serve as an independent prognosis factor of gallbladder carcinoma. The prompt of screening the ERBB signal pathway related gene mutation of the gallbladder carcinoma cancer is favorable to the gene-level diagnosis and parting of gallbladder carcinoma, the establishment of therapeutic target, the prognosis analysis and the like.

Near-infrared Raman spectroscopy can be applied to identify preneoplastic lesions of the bronchial tree. Real-time in vivo Raman spectra of lung tissues may be obtained with a fiber optic catheter passed down the instrument channel of a bronchoscope. Using prototype apparatus, preneoplastic lesions were detected with sensitivity and specificity of 96 % and 91 % respectively. The use of Raman spectroscopy apparatus and methods in conjunction with other bronchoscopy imaging modalities can substantially reduce the number of false positive results.

The invention discloses a system and a method for real-time whole body meridian detection. The system mainly comprises a server, a host and a plurality of sense units. The server is provided with a man-machine interface, a database, an image processing module and at least one display module and is connected with the host and then electrically connected with the sensor units worn on corresponding positions of a human body. The system and the method have the advantages that electronic analog signals of meridian acupuncture points can be sensed rapidly by the aid of multiple sensors arranged in the sense units, are subjected to filtering and amplification and analog to digital conversion and then transmitted to the server via the host, and are turned into graphic digital data convenient to read after processing, so that whole body physiological statuses are monitored in real time, and detection efficiency is improved greatly while medical diagnosis is facilitated.

The invention provides a kit for quantitative detection on a serum amyloid protein A. The kit comprises a horse radish peroxidase marked monoclonal antibody of a serum amyloid protein A, a carrier wrapped by the monoclonal antibody of the serum amyloid protein A, a serum amyloid protein A calibration product, and a chemiluminiscence substrate. A preparation method of the kit comprises the following steps: marking the monoclonal antibody of the serum amyloid protein A by using horse radish peroxidase; wrapping the carrier by the monoclonal antibody of the serum amyloid protein A; preparing theserum amyloid protein A calibration product by using a pure serum amyloid protein A product; subpackaging the serum amyloid protein A calibration product, the horse radish peroxidase marked monoclonalantibody of the serum amyloid protein A, and the chemiluminiscence substrate under the action of HRP (Horse Radish Peroxidase); preparing a finished product through assembling. The kit provided by the invention is high in sensitivity, good in specificity, high in quantitative detection result accuracy, low in use cost and relatively easy to popularize and apply.

The embodiment of the invention discloses a medical image segmentation method, device, equipment and system, and a computer storage medium. The method can comprise the steps of calling a target detection model to detect a candidate region where a target organ is located from a to-be-segmented medical image; calling a target positioning model different from the neural network model to position a final area where the target organ is located from the candidate areas; and calling a target segmentation model different from the neural network model to segment the final region to obtain a segmentation result of the target organ. By utilizing the technical scheme provided by the invention, full-automatic segmentation of the medical image can be realized, and a doctor can be helped to diagnose.

A peripheral blood exosome-sourced liver cancer diagnosis and prognosis marker is disclosed. The marker is exosome-sourced Rab27A protein and / or Rab27B protein. Through western blot detection, it is found that the positive expression rate of the Rab27A protein in a peripheral blood exosome of a patient with liver cancer is obviously higher than that of a common person, and the negative expression rate of the Rab27B protein in the peripheral blood exosome of the patient with liver cancer is obviously higher than that of a common person. Analysis about the abovementioned experiment results in combination with clinical data shows that content abnormity of the Rab27A protein and the Rab27B protein is significantly correlated to occurrence and development of liver cancer; and survival time of patients with positive expression of the Rab27A protein and negative expression of the Rab27B protein is shortened. The diagnosis and prognosis marker provides a method having higher sensitivity and higher specificity for liver cancer diagnosis and prognosis in clinic.

The invention belongs to the field of gene detection, and relates to a method for quickly detecting mitochondrial gene mutation or deficiency in a high-flux mode. The method is characterized in that after DNA of human whole genome is extracted, a specific primer is utilized for amplifying mitochondria DNA, interrupt response is performed after an amplified product is purified; after being recycled, a target fragment is modified and connected with a sequencing joint to form a DNA library; and data are sequenced and analyzed. The method can be used for quickly, comprehensively and simultaneouslydetecting information such as mutation and deficiency of the whole mitochondria DNA of a human body, has the characteristics of being time-saving and labor-saving, and strong in specificity, can timely find existence of mitochondria disease, and enriches a database of the mitochondria disease, so that basis can be provided for clinical diagnosis.

The invention relates to a plasma / serum circulation microRNA marker related to mlignnt melnom and application of the marker. The plasma / serum circulation microRNA marker related to mlignnt melnom is combination of miR-610, miR-1224-5p, miR-125a-3p and miR-557. The invention provides the plasma / serum circulation microRNA marker which has the capability of early diagnosis and mlignnt melnom screening and is related to mlignnt melnom, and the application of the marker.

The invention discloses a kit for detection and identification of normal plasma cell and clonal plasma cell and application thereof. The kit is matched with a flow cytometry for usage. The kit is equipped with monoclonal antibody combination according to the following substances: Ig, kappa Light Chain, Ig, lambda Light Chain, CD19, CD117, CD138, CD56, CD38 and CD45. CD38 and CD138 are first used for the identification of the plasma cells; CD45, CD56, CD19 and CD117 are employed for recognizing normal or abnormal state of the plasma cell surface; gating is conducted on normal and abnormal phenotypes (R4 and R5); and then the expression ratio of cKappa and cLambda in normal and abnormal phenotypes are respectively observed. The kit of the invention is used to identify all cPCs and has the advantages of high accuracy, fast analysis and simple method.

The invention relates to a real-time fluorescence quantitative probe and a kit for detecting IVS7-2A>G, which is one of 10 common mutations of an autosomal recessive inheritance large vestibular aqueduct-related deafness gene SLC26A4, wherein the kit comprises the real-time fluorescence quantitative probe. The wild-type probe is positioned at a site of 23403bp-23425bp; the mutant-type probe is positioned is positioned at a site of 23404bp-23425bp. In allusion to the Taqman mutant-type probe and the wild-type probe at mutation points and a pair of primers, the probes are high in specificity, high in sensitivity, and intuitive, accurate and reliable in test results, applicable to mass screening of the common mutation IVS7-2A>G of the autosomal recessive inheritance large vestibular aqueduct-related deafness gene SLC26A4 and rapid diagnosis of a SLC26A4 gene-related large vestibular aqueduct deafness.

The invention discloses a temperature measuring method, device, probe and system. The method includes: acquiring first temperature collected by a first temperature sensor and second temperature collected by a second temperature sensor, wherein the first temperature sensor is disposed in the direction, with the largest heat loss, of the first temperature sensor, and the first temperature sensor and the second temperature sensor are thermal resistance fixation; calculating the temperature difference of the first temperature and the second temperature by subtracting the second temperature from the first temperature, and acquiring the final measured temperature according to the temperature difference. The temperature measuring method, device, probe and system has the advantages that the temperature of a to-be-measured heat source can be measured accurately, simple structure is achieved, the measuring method is simple, great significance in disease confirmation or diagnosis and treatment is achieved, medical science advance and medical diagnosis are helped, and great help is provided to medical cause.

The invention belongs to the field of molecular biology, and discloses a pancreatic cancer-associated serum / plasma tsRNA marker and application thereof. The marker is tRF-Pro-AGG-004 and / or tRF-Leu-CAG-002. The tsRNA screened by the invention has significantly higher specificity and sensitivity for detection of pancreatic cancer patients than protein markers currently used in clinical practice, and greatly improves the accuracy of diagnosis. The marker can be used to prepare a diagnostic kit for auxiliary early diagnosis of pancreatic cancer.

The invention relates to a quantitative detection device for the equilibrium stability of a posture of a Parkinson's disease patient. The quantitative detection device comprises wearable wireless human body three-dimensional joint angle acquisition equipment, a signal acquisition processing and communication system arranged in the equipment, an upper data acquisition unit and computer end real-time display and storage software. The system is used for measuring joint motion data of a healthy person or the Parkinson's disease patient; by processing of acquired data, the quantitative detection device can automatically estimate whether a subject suffers from the Parkinson's disease and can also quantitatively classify the equilibrium stability of the posture of the Parkinson's disease patient, so that the level of the Parkinson's disease can be diagnosed favorably, and an accurate data support can be supplied to rehabilitation of the patient; the joint angle acquisition equipment is worn on each main joint of a human body; by completion of an appointed experiment action, estimation of the illness is completed. With the wearable characteristic, the quantitative detection device is higher in environmental suitability and practicability.

The invention discloses an intelligent elderly hypertension self-care method based on Internet. The method comprises the following steps of collecting the vital sign data of a patient and uploading toa background management system; through using traditional Chinese medicine time and a pulse condition, and a recipe, providing a full-day diet and daily life guidance service, through the method of combining quantification and quantization, form an electronic diary for each patient, and transmitting to a corresponding community hospital to form a systematic file management; publishing the healthcalendar of an elderly inpatient with hypertension through a background management system, refining and quantifying the intakes of staple food, vegetable, fruit, oil and fat, and listing the health calendar of a hypertensive patient with other disease types separately; and prompting daily medication time and the generated diet and exercise plan of the day for the patient. The self-care state of the elderly hypertensive patient is tracked, investigated and assessed through the Internet, and regular intervention is performed, which is good for improving the life quality of and the health state of the elderly patient.

The invention provides a hybridoma cell strain of NNMT protein monoclonal antibody, a monoclonal antibody secreted by the cell strain and application. The monoclonal antibody of the invention has a high antibody titer, affinity and high purity after purification to NNMT proteins, thus indicating that the monoclonal antibody can be applied in the scientific research field of NNMT proteins as well as in clinic detection for immunohistochemistry of pathological sections of different pathologic tissues to detect expression of NNMT proteins in the tissues. Therefore, the monoclonal antibody provided in the invention contributes to diagnosis, treatment and prognosis of NNMT associated tumors.

The present invention relates to a multifunctional integrated anal tube drainage device. The multifunctional integrated anal tube drainage device comprises a drainage main pipe 1 for loose stool drainage, a liquid injection branch pipe 2 for injecting medicinal liquid or rinsing liquid and attached to an inner side wall of the drainage main pipe 1, a viscous exudate absorption sticker 9 for positioning, a guide wire 7 for dredging and an anal temperature measuring instrument. The integrated anal tube drainage device can be used for loose stool drainage and anorectal administration of patients,is good in positioning and fixing effects, and not liable to prolapse, reduces incidence of incontinence-associated dermatitis, avoids stool pollution and cross infection, reduces suffering of the patients and helps to achieve effective cooperation with medical care work.

The present invention discloses a GFAP detection method based on a fluorescence resonance energy transfer method, wherein a micro-pore plate is coated with a purified glial fibrillary acidic protein (GFAP) antibody to prepare a solid-phase antibody, glial fibrillary acidic protein (GFAP) is sequentially added to the monoclonal antibody-coated micro-pores, combination with Alexa Fluor488 labeled and Alexa Fluor594 labeled GFAP antibody to form an antibody-antigen two-fluorescent-labeling-antibody complex, absorbance (OD value) is determined by using a fluorescent enzyme analyzer according to the FRET principle, and the human glial fibrillary acidic protein (GFAP) content in the sample is calculated by using a standard curve. According to the present invention, with the use of the three kinds of the antibodies to simultaneously identify, such that the specificity is high, the accuracy is good, the detection time is substantially shortened compared to the existing method, the reference information is provided for the clinical diagnosis, the clinicians can be helped to diagnose and assess the progression of stroke, and the prognosis of patients can be improved.

The invention provides an optical coherence tomography angiography method and device. The method comprises the steps: collecting OCT original data of a retina, obtaining B-scan images, and carrying out registration on each group of images; calculating blood flow information of a coordinate point according to the square of the difference value of the maximum image value and the minimum image valueon the same coordinate point in each group, and further obtaining a blood flow signal graph corresponding to the current group; synthesizing each group of images into a high-definition image and layering the high-definition images; and , obtaining the OCTA among the layers according to the blood flow signal graph and the layering result. Compared with the prior art, the influence on the noise partis small, the blood flow signal is enhanced, and the discrimination between the blood flow signal and the noise is increased, so that the quality of the obtained angiography image is higher, capillary details are richer, and therefore a doctor can diagnose fundus diseases of a patient easily. Meanwhile, the algorithm complexity is low, the calculation amount is small, the burden of a computer isreduced, the calculation speed of the blood flow signals is increased, and therefore the working efficiency is improved.

The invention discloses human TSHR extracellular fusion protein and a preparation method thereof. The N end of the human TSHR extracellular fusion protein is connected with exogenous secretion signal peptide, and the C end thereof is connected with a purification marker sequence; when the fusion protein is expressed and purified, the fusion protein the N end of which is connected with the exogenous secretion signal peptide is resected, and the specific amino acid sequence is shown in SEQ ID No.1. The invention obtains high-expression soluble recombinant protein with the antigenic secretion form of TSH receptor in a good in-vitro expression system by constructing recombinant pichia pastoris containing pPICZ alpha A-hTSHRecd expression vector.