The invention belongs to the medical field of genetic engineering and tumors, and discloses an SNP marker related to auxiliary diagnosis of lung cancer of non-smoking women and applications thereof. The marker is a combination of 25 SNPs such as rs10051970, rs10087140, rs10483845, rs11233751, rs11643408, rs11732247, rs1255485, rs12612657, rs11643408, rs11732247, rs16935064, rs12612657, rs1535271, rs166402, rs16935064, rs12612657, rs1535271, rs166042, rs16935064, 17040797, rs17073021, rs17623941, rs17670695, rs2550911, rs40395, rs4454390 and rs4640379. The marker can be used for preparing a kit for the auxiliary diagnosis of lung cancer of non-smoking women, and applications thereof.