8results about How to "Stable expression" patented technology

This invention relates to the field of natural language processing technology, and more particularly to a method and system for language transfer recognition based on cultural semantic embedding. The method includes the following steps: acquiring source language data and target language data; performing semantic isomorphic centering on the source and target language data to obtain semantically equivalent data; decoupling pragmatic features based on the semantically equivalent data to obtain pragmatic feature data; performing contrastive cultural latent space embedding based on the pragmatic feature data to obtain bilateral cultural representation data; performing cultural modality affine transformation based on the bilateral cultural representation data to obtain cultural difference data; performing implicit distortion pattern discrimination based on the cultural difference data to obtain cultural semantic distortion data; and generating transfer results based on the cultural semantic distortion data to obtain language transfer result data. This invention decouples pragmatic features under semantic equivalence constraints and achieves language transfer recognition through cultural semantic embedding and modality affine modeling.

The invention discloses an intestinal bacterium-derived melatonin synthetase and an application thereof. Specifically, the melatonin synthetase is selected from one or more of a DDC enzyme, an AANAT enzyme and an AST enzyme, the DDC enzyme comprises an amino acid sequence as shown in SEQ ID NO.2 or an active fragment of the amino acid sequence, or an analogue of which the similarity with the amino acid sequence as shown in SEQ ID NO.2 is more than 80%, and the DDC enzyme comprises an amino acid sequence as shown in SEQ ID NO.2 or an active fragment of the amino acid sequence as shown in SEQ ID NO.2 or an analogue of which the similarity with the amino acid sequence as shown in SEQ ID NO.2 is more than 80%. The AANAT enzyme comprises an amino acid sequence as shown in SEQ ID NO.34 or an active fragment thereof, or an analogue of which the similarity with the amino acid sequence as shown in SEQ ID NO.34 is more than 80%, and the AST enzyme comprises an amino acid sequence as shown in SEQ ID NO.42 or an active fragment thereof, or an analogue of which the similarity with the amino acid sequence as shown in SEQ ID NO.42 is more than 80%. On one hand, the melatonin synthetase can be used for improving the melatonin level of a host and improving related diseases; on the other hand, the method can be used for biosynthesis of melatonin and has a good application prospect.

The invention discloses a DNA structure for saccharomyces cerevisiae single-cell barcode labeling and chromatin state conjoint analysis and application thereof, and belongs to the technical field of molecular biology and single-cell multi-omics analysis. From the 5'end to the 3 'end, a fluorescent protein gene, a Tn5ME structure, a reverse transcription primer binding region, a CYC1 terminator fragment and a linearized shuttle plasmid skeleton are subjected to homologous recombination, and a pCM carrier is obtained; after NdeI / BamHI enzyme digestion, oligonucleotides with random sequences, which are subjected to annealing, amplification, Klenow filling-in and enzyme digestion, are connected, and transformation extraction is carried out, so that the DNA structure for saccharomyces cerevisiae single-cell barcode labeling and chromatin state combined analysis is obtained. On the basis of the constructed DNA structure, ATAC-seq and a bar code reverse transcription method are combined, important data related to the transcription state and chromatin state of a single cell can be obtained at the same time through bar code information, single cell pedigree tracking of the saccharomyces cerevisiae is achieved, and research on single cell epigenomics of the saccharomyces cerevisiae and other microorganisms is promoted.

This invention relates to the use of a gene carrier as a drug delivery carrier, the gene carrier comprising metal nanoparticles and a double-stranded nucleic acid molecule bound to the surface of the metal nanoparticles. The invention particularly relates to the use of the carrier for treating metabolic diseases, and to pharmaceutical compositions for the prevention or treatment of metabolic diseases, the pharmaceutical compositions comprising the carrier.

The application provides a construction method and application of recombinant escherichia coli for efficiently preparing high-purity sialyllactose-N-tetraose a. The application precisely integrates genes encoding beta-1,3-N-acetylglucosamine glycosyltransferase, beta-1,3-galactosyltransferase and alpha-2,3-sialic acid glycosyltransferase in the genome of escherichia coli BL21 (DE3), knocks out endogenous competitive metabolic genes, and optimizes the metabolic pathways of precursor substances UDP-acetylglucosamine, UDP-galactose and CMP-N-acetylneuraminic acid, effectively reduces the generation of non-target byproducts including lactose-N-triose, lactose-N-tetraose and 3'-sialyllactose, and successfully constructs a recombinant chassis strain capable of efficiently producing high-purity sialyllactose-N-tetraose a. Fermentation culture of the constructed engineering bacteria can efficiently prepare high-purity sialyllactose-N-tetraose a under the premise of guaranteeing biosafety, and is suitable for the production demand in the food field.

The invention relates to the technical field of gene therapy, in particular to a method for knocking out a Bax gene on the basis of an HEK293 cell line by utilizing a CRISPR / Cas9 technology, constructing a stable cell line expressed by foreign protein and improving the AAV packaging efficiency. The invention discloses a Bax2.6 cell strain. A pro-apoptosis factor Bax gene is knocked out from an HEK293T cell. Compared with a wild type HEK293T cell, the Bax2.6 cell strain provided by the invention shows higher cell activity under various stress conditions, the cell survival rate of the Bax2.6 cell strain is improved by at least 20% under the treatment conditions of small molecule inhibitors ZV-FMK, Nec-1, puromycin (Puro), cis-platinum (CDDP) and rotenone, and the Bax2.6 cell strain shows excellent environmental tolerance and production robustness. When the Bax2.6 cell strain provided by the invention is used for transient transfection and packaging of an adeno-associated virus (AAV) vector, the copy number of the obtained AAV virus can be up to 2 * 10 copies / mL, and the AAV packaging efficiency is improved by more than 10 times compared with that of a conventional HEK293T cell line.

The application provides a fusion protein for antibody site-specific coupling and application thereof. The fusion protein of the application is mainly fused by an antibody Fc affinity protein binding monomer and an avidin protein, realizes direct coupling of the antibody Fc affinity protein and a biotin-labeled molecule, and no longer uses a click chemistry scheme, so that the production and use difficulty of the antibody Fc affinity protein Protein G is greatly simplified, the Protein G protein no longer needs to be activated through the click chemistry in the production, the transportation and storage conditions no longer need to be strictly controlled, and even one-step antibody molecule coupling can be realized.

The invention discloses a recombinant antigen protein, a recombinant vector, a recombinant host cell and application thereof and a vaccine, and relates to the technical field of antigen preparation, the recombinant antigen protein comprises a capsid protein of porcine circovirus type 2 and a polypeptide in a capsid protein of porcine circovirus type 3; the polypeptide in the porcine circovirus type 3 capsid protein comprises B cell neutralization site polypeptide and / or T cell neutralization site polypeptide. The recombinant antigen protein disclosed by the invention can react with PCV2 type positive serum and PCV3 type positive serum at the same time; the vaccine prepared by mixing the recombinant antigen protein with an adjuvant is an efficient bivalent vaccine, and can effectively stimulate a pig body to generate high-concentration specific antibodies of PCV2 and PCV3, so that a reference is provided for joint prevention of the two viruses. The recombinant antigen protein has the characteristics of stable expression, high yield and good immunogenicity.