Installation method of fluorescent visual high-transfer human liver cancer nude mouse model

Abstract

The invention belongs to the field of microorganism animal cell system, and relates to an establishment method of a fluorescence visualization human hepatoma nude mice model which can self emit high strength red or green fluorescences and has the metastatic ability. After obtaining a human high-metastatic fluorescence hepatoma cell line HCCLM3-R, HCCLM3-G, HCCLM6-R and HCCLM6-G of fluorescence genes with high chromosome conformable degree, high fluorescence intensity and stable expression by using the method of pseudotype slow virus infection, hepatoma cells expressed by the fluorescences is placed beneath nude mice skin to establish a fluorescence visualization high-metastatic human hepatoma nude mice subcutaneous tumor model or subcutaneous fluorescence expression tumor tissues is placed in nude mice hepar to establish a fluorescence visualization high-metastatic human hepatoma nude mice hepar primary tumor model. The got model can be used as a tracer of animal in vivo hepatoma cells, and can be used for molecular mechanism study of hepatoma recurrence and metastatic and prophase therapeutic effect discrimination for new anti-hepatoma therapy and new anti-hepatoma drugs, etc.

Description

technical field [0001] The invention belongs to the field of microbial animal cell lines, and relates to a method for establishing a nude mouse model of highly metastatic human liver cancer, in particular to a method for establishing a nude mouse model of human liver cancer with visible red or green fluorescence and high metastatic ability. Background technique [0002] Primary hepatocellular carcinoma is one of the most common malignant tumors in my country and even in the world. In my country, about 200,000 people die of liver cancer every year, accounting for about half of the global liver cancer deaths. Primary hepatocellular carcinoma has increasingly become a major malignant disease that seriously affects people's health in our country. In recent years, the governments of various countries, relevant medical and scientific research institutions, and drug R&D companies have increased their clinical and basic research on primary hepatocellular carcinoma and the developme...

AI Technical Summary

Problems solved by technology

It may be that the onset of liver cancer is hidden, and tumor cells are easy to disseminate early; traditional therapy cannot completely eliminate liver cancer cells in the patient's body; traditional therapy has relatively large toxic and side effects, which limits its repeated clinical application; The mechanism is unknown

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