9results about How to "Good experimental repeatability" patented technology

The application relates to an anti-GCDFP-15 monoclonal antibody and a preparation method and application thereof, and belongs to the technical field of immunology. The anti-GCDFP-15 monoclonal antibody provided by the application has the amino acid sequence of a heavy chain variable region as shown in SEQ ID NO. 1 and the amino acid sequence of a light chain variable region as shown in SEQ ID NO. 2. The nucleotide sequences of the heavy chain variable region and the light chain variable region of the monoclonal antibody are also provided, and the monoclonal antibody can be prepared by using genetic engineering technology. The monoclonal antibody prepared by the application has the advantages of stable property, high experimental repeatability and high titer, is suitable for immunological detection, and can specifically recognize the GCDFP-15 protein expressed in human skin and breast tissue.

The application discloses a kind of super-low freezing point condensate oil freezing point test experimental device and method, the experimental device includes support system, cooling system, sample placement system, temperature testing device;The sample placement system includes glass test tube and the sealing plug of sealing the glass test tube, the glass test tube is used to hold condensate oil to be tested, and the lower part of the glass test tube is equipped with three parallelly arranged scale lines A, B, C from bottom to top;The temperature testing device is used for testing the temperature of the condensate oil to be tested by passing through the sealing plug;The cooling system includes glass sleeve, isolation glass bottle, open glass bottle, the glass test tube is arranged in the glass sleeve, the glass sleeve is arranged in the isolation glass bottle, and the isolation glass bottle is arranged in the open glass bottle;The support system has height adjustment and rotation function.The application can predict freezing point in advance, and can efficiently test the freezing point of super-low freezing point condensate oil.

The invention discloses a device and a method for automatically identifying a near-dry state of a solution in a small chamber. The device comprises a camera, an annular light source, a base, a heating part, a photoelectric sensor and a temperature sensor, the heating part is located on the base, and the camera and the circumferential light source are located at the top of the heating part; the annular light source emits a light beam with a specific wavelength to vertically irradiate a sample solution in the heating part, the photoelectric sensor receives a transmission light signal / reflection light signal penetrating through the solution, and the temperature sensor synchronously collects the temperature of the heating part. The method is suitable for a radioactive metal oxide powder sample pretreatment scene. The method can objectively and accurately judge that the solution is evaporated and concentrated to the nearly dry state in real time, and manual intervention is not needed.

A skeletal muscle injury experiment device relates to the technical field of medical experiment equipment and comprises an experiment table, a bracket, a sleeve, a sliding rod, a balancing weight, an experiment piece and a locking piece, the support is fixedly connected to the experiment table, and the sleeve is connected to the support. The two ends of the sliding rod are detachably connected with the balancing weight and the experiment piece. The sleeve is provided with a sleeve cavity; the sliding rod is slidably connected with the sleeve, and at least part of the sliding rod is located in the sleeve cavity; the balancing weight is located outside the cavity of the sleeve. The locking piece is connected with the sleeve and the sliding rod, the locking piece can enable the sliding rod to be locked at the preset height of the sleeve, and the locking piece is configured to be capable of unlocking the sliding rod when being opened, so that the sliding rod moves along the cavity of the sleeve under the action of gravity and drives the experiment piece to downwards abut against or approach the experiment table. The utility model provides a skeletal muscle injury experimental device, which aims to solve the technical problems of complicated operation, poor experimental repeatability and poor stability of experimental results in the prior art.

The application provides a reagent mixing processor for medical immunological detection, which comprises a workbench and a driving unit, the driving unit drives a bearing shell to swing in a circular trajectory in the horizontal direction; the bearing shell is located on the top of the workbench and is overlapped with an annular bearing plate on the top of the workbench shell, the bearing plate is used for supporting the bearing shell, and the driving unit does not bear the supporting action; a fixing assembly for fixing a reagent container, the fixing assembly is several, and the several fixing assemblies are uniformly distributed on the bearing shell; the fixing assembly clamps the middle or middle upper part of the container, and with the swinging of the bearing shell, the container swings in a circular trajectory with the clamping point as the center. The device realizes the circular swing of the bottom of the container by driving the bearing shell to swing in a circular trajectory, simulates the action of gently swinging the test tube manually, and realizes efficient and gentle mixing without high-speed stirring or magnetic auxiliary.

The application discloses a kind of molecular inversion probes, molecular inversion probes based on mandarin fish genotyping chip and application, it is related to fish molecular breeding technical field, the molecular inversion probe of the present application sequentially includes left arm specificity sequence, general skeleton sequence and right arm specificity sequence from 5'to 3 ', general skeleton sequence includes molecular tag for high-throughput sequencing;The present application also provides the application including genotyping chip and in mandarin fish genotyping.The present application successfully designs and selects 10,000 specific probes, by verification, the chip system average enrichment success rate reaches 92.3%, and 53,454 high-quality single nucleotide polymorphism sites are captured, sequencing data comparison rate reaches 91.96%, target area is evenly covered, with the characteristics of high accuracy, high uniformity and high cost performance, provides efficient and reliable technical solutions for the molecular breeding and population genetics research of mandarin fish even other aquatic economic species.

The invention discloses an in-situ measurement device and method for material viscosity, and belongs to the technical field of precise rheological measurement and acoustochemical process analysis. The in-situ measuring device for the material viscosity comprises a main box body which is hollow, and a measuring groove for storing a material to be measured is formed in the top of the main box body; the ultrasonic generation unit comprises a plurality of ultrasonic transducers and a plurality of rigid conduction rods; the temperature control unit comprises a heating element attached to the outer wall of the measuring groove and a temperature sensor arranged at the bottom of the groove, and is used for keeping the temperature of the material constant; the control unit comprises a processor and a human-computer interaction interface, the processor is arranged in the main box body, and the main box body, the ultrasonic generation unit, the rotary viscosity measurement unit, the temperature control unit and the control unit realize in-situ viscosity measurement under the action of ultrasonic waves through integrated design; the transducer and the titanium alloy conduction rod which are arranged in an axial symmetry mode are adopted to generate a uniform ultrasonic field.

The invention discloses a 3D hepatotoxicity test monodisperse microgel preparation method, a 3D hepatotoxicity test monodisperse microgel preparation system and a medium, and belongs to the technical field of 3D cell culture and drug screening. The system comprises a preparation module, a statistical analysis module, a material processing module and a control system, the method comprises the following steps: collecting historical data to train an adaptive neural network, and establishing a mapping relation between control parameters and microgel quality indexes; a neural network is used as a prediction model, and optimal control input is solved through a model prediction controller; performing microgel preparation; acquiring images in real time to obtain and feed back actual particle sizes and variation coefficients; applying the new data to incremental updating of the neural network; and performing iteration to form a closed-loop optimization process. According to the method, multi-parameter self-adaptive optimization is realized through an artificial intelligence algorithm, the problem of manual exploration of parameters of different hydrogel materials is solved, the monodispersity and consistency of microgel are improved, and full-process automation is realized.

This invention relates to the field of marine clean energy simulation technology, and discloses a rapid indoor simulation non-destructive evaluation method for hydrate-containing sediments, comprising the following steps: S1, preparing an indoor simulation system for simulation: the indoor simulation system includes a sample saturation component, a sample component, an acoustic testing component, a temperature control component, and a data acquisition component; S2, preparing and saturating the sample. This invention can rapidly generate hydrates and directly prepare saturated sediment samples under indoor conditions, significantly shortening the sample preparation cycle; it avoids hydrate decomposition or structural disturbance during saturation, improving sample structural stability and experimental repeatability; the sample remains within the ring cutter throughout the preparation and testing process, eliminating the need for demolding and significantly simplifying the experimental procedure; it achieves non-destructive evaluation of hydrate saturation through acoustic testing, and can combine temperature monitoring to reflect the internal phase transition process and latent heat effect of the sample in real time.