The invention discloses a 3D hepatotoxicity test monodisperse microgel preparation method, a 3D hepatotoxicity test monodisperse microgel preparation
system and a medium, and belongs to the technical field of 3D
cell culture and
drug screening. The
system comprises a preparation module, a
statistical analysis module, a material
processing module and a
control system, the method comprises the following steps: collecting historical data to
train an adaptive neural network, and establishing a mapping relation between
control parameters and microgel quality indexes; a neural network is used as a prediction model, and
optimal control input is solved through a
model prediction controller; performing microgel preparation; acquiring images in real time to obtain and feed back actual particle sizes and variation coefficients; applying the new data to incremental updating of the neural network; and performing iteration to form a closed-
loop optimization process. According to the method, multi-parameter self-
adaptive optimization is realized through an
artificial intelligence algorithm, the problem of manual exploration of parameters of different hydrogel materials is solved, the monodispersity and consistency of microgel are improved, and full-process
automation is realized.