71 results about "3D cell culture" patented technology

The invention discloses a high-stability and low-sensitization 3D cell culture meat scaffold and a preparation method thereof, and belongs to the field of future food science and technology processing. The fish gelatin sodium alginate cell culture meat scaffold is prepared through covalent modification of sodium alginate with different concentrations, the problem that fish gelatin gel is unstable at 37 DEG C is remarkably improved, the gel strength is improved by about 282.42%, the hydrophilicity is remarkably improved, and the cell culture meat scaffold is suitable for being used for preparing the fish gelatin sodium alginate cell culture meat scaffold. Meanwhile, the release amount of mouse plasma histamine of intragastric sodium alginate covalently modified group fish gelatin is reduced by about 54.35%, and the release rate of beta-hexosaminidase of RBL-2H3 cells is reduced from 27.50% to 11.31%. The high-stability and low-sensitization cell culture meat scaffold is prepared from fish gelatin, sodium alginate and calcium chloride, the problem of low stability of a single fish gelatin cell scaffold is solved, and the potential sensitization risk of the cell culture meat scaffold is reduced.

The invention belongs to the technical field of biology, and particularly relates to an endogenous cell aggregate source exosome and application thereof.The exosome is obtained by culturing mesenchymal stem cells in a 3D cell culture system added with a cell culture medium and IGF-1 to obtain mesenchymal stem cell aggregates and secreting the mesenchymal stem cell aggregates in a culture medium; the cell culture medium is prepared from the following components in parts by weight: 1 to 3 parts of hE-cad-Fc and 1 to 3 parts of hN-cad-Fc. The preparation method of the mesenchymal stem cell source exosome pretreated by combining the cadherin with the IGF-1 has extremely high exosome production efficiency, and the prepared mesenchymal stem cell source exosome pretreated by combining the cadherin with the IGF-1 is complete in structure, uniform in particle size distribution, high in production efficiency and high in effective functional component content.

A method for monitoring a process of producing a multicellular structure from a population of cells in a 3D cell culture is described. The method comprises obtaining one or more 3D images of the 3D cell culture obtained by imaging the cell culture at different depths and / or from different angles, processing the one or more images to obtain a plurality of image-derived features, and determining values of one or more indicators indicative of progress or outcome of the maturation process using a statistical model, the statistical model is adapted to predict values of one or more indicators indicative of progress or outcome of the maturation process using an input comprising a plurality of image-derived features.

The invention relates to the technical field of biology, in particular to a matrix-enabled mesenchymal stem cell aggregate and application, and the matrix-enabled mesenchymal stem cell aggregate is obtained by subculturing matrix-enabled mesenchymal stem cells in a 3D cell culture system. The matrix-enabled mesenchymal stem cells are obtained by subculture of mesenchymal stem cells in a cell culture matrix, and the cell culture matrix is prepared from 1-3 parts of hE-cad-Fc and 1-3 parts of hN-cad-Fc. Endogenous epithelium cadherin and nerve cadherin are highly expressed in cell aggregation cells, so that transcription factors are promoted to enter nuclei, the expression levels of anti-inflammatory immune regulation related factors and matrix degradation and cell migration related factors are up-regulated, and activated macrophages are promoted to be polarized to M2 type macrophages; the effects of anti-inflammation, immune regulation, tissue damage repair and the like of intravenous implantation of the matrix-enabled mesenchymal stem cell aggregate for treating diseases such as hepatic fibrosis, diabetes mellitus, nerve injury and the like are remarkably improved.

The invention relates to an automatic microfluid cell culture system and method integrated with artificial intelligence, and belongs to the field of biomedical engineering. Through a modular micro-fluidic chip and an advanced monitoring algorithm, efficient and automatic management of a cell culture process is realized. The system analyzes cell behaviors in real time by utilizing machine learning, automatically adjusts culture conditions, and ensures consistency and stability of cell growth. The application of the digital microfluidic technology allows accurate control of single cells or microspheres, and optimizes the high-throughput screening process. In addition, the system supports 3D cell culture and organoid model construction, and provides an experimental platform closer to physiological conditions. Through integration of automatic operation and remote monitoring functions, cell culture is more convenient and controllable, and the efficiency and accuracy of biomedical research and drug screening are remarkably improved. The design of the system pays attention to environmental protection and sustainability, resource consumption is reduced, and innovativeness and practicability in a cell culture technology are shown.

The invention relates to the technical field of 3D cell culture materials, in particular to a preparation method of a 3D cell culture material, which comprises the following steps: S1, raw material preparation: raw materials comprise sodium alginate, chitosan, polyvinyl alcohol and a phosphate buffer according to a mass ratio of 1: (0.6-0.8): (0.1-0.3): (1-3). According to the invention, the porous structure is also beneficial to enhancing the mechanical properties of the material, after the chitosan is pretreated, the molecular chain of the chitosan can be stretched, the compatibility with other components is better, the formation of a stable network structure is facilitated, and the gelatin and the genipin are subjected to a cross-linking reaction through ultraviolet irradiation to form a three-dimensional network structure; the cross-linked structure can improve the mechanical property of the material, so that the material has better stability and deformation resistance, and the use performance of the material is effectively improved.

The invention relates to a cellulose-based hydrogel prepared based on a cellulose copolymer, a cellulose derivative copolymer or a blend thereof, ultramicro fibers of cellulose form a net structure, and the cellulose-based hydrogel has a highly-ordered three-dimensional micro-nano structure, is better in transparency and is more beneficial to observation of a cell state. The cellulose-based hydrogel is suitable for culture of 3D cell culture tissues and organoids, the stem cells cultured by the cellulose-based hydrogel can form cluster culture stem cells and organoids, the operation is simple, compact fused monolayer endoderm cells are formed, non-directional differentiation of the stem cells can be inhibited, and the formed organoids are thick in vesicle wall and are in a solid shape. And the obtained product is a differentiated mature organ and can be used for high-throughput drug screening, drug toxicity testing and regenerative medicine.

A set of 3D cell culture colloids and a method for 3D cell culture therewith are provided. [Solution] The 3D cell culture colloid set includes gel material A, buffer solution C, and buffer solution D. The 3D cell culture method includes the steps of adding cells to a mixture containing gel material A, allowing it to react at low temperature to obtain a colloid containing the cells, adding buffer solution C to the colloid to crosslink it, removing buffer solution C and adding a growth medium, and culturing the cells until spheroids are formed within the colloid. The present invention also further dissolves the colloid with buffer solution D, and extracts and analyzes the cells after culturing. Therefore, the 3D cell culture colloid set of the present invention is easy to use and can be used for 3D cell culture of a variety of cell lines.

Provided herein are 3D bioassays to measure antibody-dependent cell-mediated cytotoxicity (ADCC). In particular, provided is a method for assessing ADCC comprising contacting an antibody or a functional antibody fragment thereof with a target cell and an effector cell expressing FcR, in a 3D cell culture under a condition allowing the interactions between those substances; and detecting the changes in the expression levels of biomarkers CXCL9, CXCL10 and CXCL11 and 0, 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 of the biomarkers selected from UBD, IDOL, STEAP4, JAG1, APOL4, GBP4, CD274, GBP5, CCL3 and CCL4; and assessing ADCC of the antibody based on the expression results. Also provided are the use and product relate to the method.

A microcavity bioreactor is provided that allows cell growth and cell differentiation in the same vessel, such that higher order three-dimensional cell cultures such as organoids can be generated in a singular vessel. The microcavity bioreactor may be part of a microcavity bioreactor system that allows for perfusion based cell culture and perfusion based cell harvesting. The microcavity bioreactor includes at least one, and preferably at least two, fluid distributor structures in the housing vessel of the microcavity bioreactor.

The present invention relates to the technical field of organoid culture, and discloses a method for culturing goose kidney organoids, wherein the method firstly takes goose embryos, separates primary kidney tissues, washes, crushes, digests, filters, and washes twice to obtain primary cells, then mixes the primary cells with matrix gel, coagulates, obtains a colloidal mixture, and then places the mixture in a culture medium containing goose serum and cultures it for at least 10 days to obtain goose kidney organoids; wherein, the method comprises: 4 The primary cells are mixed with 70 μL of matrix gel and solidified to obtain a colloidal mixture. Through the above design, the goose kidney organoid obtained in this application is a 3D cell culture system, which is highly similar to the tissue or organ derived from the body, can replicate the complex spatial morphology of differentiated tissues, and can show the interaction between cells and cells, and cells and matrix. In addition, the application also discloses a goose kidney organoid and its application.

A composition may be useful for a 3D printing support or a 3D cell culture support and be excellent in salt tolerance, including a (A) a polymer having a structural unit of formula (1)R1 and R2 independently being a hydrogen atom or an alkyl group having 1 to 10 carbon atoms, or R1 and R2 together optionally forming a ring structure having 3 to 10 carbon atoms; and (B) an aqueous medium.

The invention relates to a stable sericin biopolymer composition comprising a sericin, a cross-linking agent and optionally a catalyst. The stable sericin biopolymer composition is prepared by freezing-thawing process. The process of cross-linking sericin using the freeze- thaw cycle does not require curing at high temperature or under strong pH thus preventing the damage to the sericin proteins. The stable sericin biopolymer is used for preparation of cost effective sericin substrate platform such as gels, films, scaffolds and electrospun films for 3D cell culture and other biomedical applications.

The present invention is directed to a method for ex-vivo-engineering of cells, in particular stem cells or T cells, preferably hematopoictic stem and / or progenitor cells, mesenchymal stem cells, or T cells comprising a step of culturing the cells on a three-dimensional scaffold. The method of the invention is capable of improving the efficiency of genetic modification of cells and the functionality of the engineered cells.

The present invention relates to fibroblast growth factor 2 (FGF-2) polypeptides which have improved stability as compared to wild-type FGF-2 while maintaining biological activity. The invention provides a preparation method and application thereof in biotechnology research and industrial application, medicine, pharmaceutical industry, cosmetics, clean meat industry, organoid generation and 3D cell culture models and other related applications.

The present invention provides an apparatus and method for in-well high throughput 3D cell culture, the 3D orifice plate plug-in perfusion bioreactor comprising: an inlet in fluid communication with a first chamber and an outlet in fluid communication with a second chamber; and a plurality of hairy structures extending downwardly from the lower surface of the bioreactor, each hairy structure comprising a supply channel fluidly communicating the first chamber with the hairy structure tip, and a return channel fluidly communicating the second chamber with the hairy structure tip, wherein the supply channel and the return channel are in fluid communication with each other at the tip of the hairy structure. According to the invention, the real biological environment of cells in vivo can be better simulated, which is crucial for realizing accurate drug screening and does not need to sacrifice the high throughput advantage of traditional 2D culture plate screening.

A gellable material for encapsulating a cellular aggregate in a well plate is provided. The cellular aggregate, before or after encapsulation, can be organoid or a spheroid, and typically, the cellular aggregate, once encapsulated, has substantially no movement during media exchange, handling of the well plate or tilting of the well plate. Methods for producing encapsulated cellular aggregates, and uses thereof are also provided.

The utility model discloses a 3D cell culture chip structure which comprises a chip support and chip bodies, a base is fixedly installed at the bottom of the chip support, partition plates are symmetrically installed in the chip support, and the 3D cell culture chip structure further comprises positioning assemblies which are symmetrically arranged on the two sides of the plurality of chip bodies in the chip support, the positioning assembly comprises two groups of sliding grooves which are symmetrically formed in the upper portion of the interior of the chip support in a penetrating mode and located on the two sides of the chip body, positioning blocks are slidably connected to the interiors of the two groups of sliding grooves, two groups of sliding rods are fixedly installed on the sides, away from each other, of the two groups of positioning blocks correspondingly, and the sliding rods are slidably connected with the sliding grooves; one sides of the exteriors of the two groups of sliding rods are sleeved with telescopic springs, and one ends of the telescopic springs are fixedly connected with the chip bracket; and a user can conveniently place and take down the chip body, the chip body can be conveniently positioned, the practicability and convenience are improved, and the practicability is improved.

The invention belongs to the technical field of biology, and particularly relates to a mesenchymal stem cell aggregate jointly energized by matrix and cell factors and application of the mesenchymal stem cell aggregate. The aggregate is prepared by culturing mesenchymal stem cells in a culture medium added with a cell culture matrix and IGF-1 (Insulin-like Growth Factor-1) to obtain pretreated mesenchymal stem cells; and continuing subculture in a 3D cell culture system to obtain the cadherin cell culture medium, wherein the cadherin cell culture medium is prepared from 1-3 parts of hE-cad-Fc and 1-3 parts of hN-cad-Fc. The obtained aggregate is complete in structure and clear in boundary, cell endogenous cadherin and phosphorylated IGF-1 receptors are highly expressed, and the curative effect of treating refractory and repeated immune disorder diseases, such as systemic sclerosis, xerosis and systemic lupus erythematosus, with complex etiology is remarkably improved.

Disclosed herein is a perfusable scaffold for used in a three-dimensional cell culture and method of fabrication of a perfusable three-dimensional cell culture containing a perfusion scaffold. Also disclosed herein are the perfusable three-dimensional cell cultures and uses thereof.

The invention discloses a preparation method of a 3D cell culture body. The preparation method comprises the following steps: (A) constructing a framework based on a sacrificial material; (B) arranging a first culture cell and a curable biological medium around the skeleton, and then curing the biological medium to form a cured biological medium block; (C) covering the biological medium block with a curable packaging material, then curing the packaging material, and forming a packaging layer on the outer side of the biological medium block; and (D) removing the skeleton so as to form a perfusion structure in the biological medium block, thereby obtaining the 3D cell culture body.

The utility model discloses a novel culture mould for 3d cell culture capable of being used for toxicological research, which belongs to the technical field of cell culture moulds and comprises a micropore mould, a partition mould is arranged on the micropore mould, a plurality of culture chambers are arranged inside the partition mould, a plurality of micro holes are arranged on the culture chambers, and the micro holes are communicated with the micropore mould. According to the three-dimensional cell culture mould, a plurality of cells with different drug concentrations can be cultured in the same mould at the same time, so that the operation amount and complexity of an experiment are greatly reduced, the preparation operation process of a three-dimensional cell structure is simplified, and the production efficiency is improved. According to the invention, the experimental error is reduced, the drug concentration can be ensured not to interfere with each other between partitions, the experimental efficiency is improved, a plurality of samples can be treated in the same mold at the same time, the experimental cost is reduced, and the consumption of reagents and materials is reduced.

The present invention relates to a pharmaceutical composition for preventing or treating cartilage disease or inflammatory joint disease and a method for preparing a chondrospheroid having chondrocyte differentiation ability. The present invention provides a pharmaceutical composition comprising a stem cell-derived spheroid as an active ingredient for preventing or treating cartilage disease or inflammatory joint disease, and a method for preparing a chondrospheroid having chondrocyte differentiation ability, the method comprising the steps of: Inoculating stem cells into a three-dimensional (3D) cell culture vessel made of a plastic material; treating the stem cells inoculated into the 3D cell culture vessel with a chondrocyte differentiation-inducing factor; culturing the stem cells treated with the chondrocyte differentiation-inducing factor to form chondrospheroids; and separating the formed chondrospheroids.

The invention provides a chip model for detecting whether candidate drugs can cause cytokine storm, and belongs to the technical field of biomedicine. The chip model comprises a culture plate, the culture plate comprises a plurality of culture units, and each culture unit comprises a culture solution channel and a plurality of cell growth holes communicated with the culture solution channel; the bottom of the cell growth hole is lower than the bottom of the culture solution channel to form a growth hole concave part. According to the chip model, medicine combined immune cell killing can be carried out while 3D cell culture is realized, and whether side reaction is generated or not is detected in subsequent experiments. According to the application, higher-throughput culture and detection can be carried out, the experimental purpose of detecting and evaluating a plurality of anti-tumor drugs on the same chip is achieved, and drug safety evaluation can be carried out through components of a culture solution while drug effect evaluation is carried out.

Multi-well plate compatible device or insert for three-dimensional (3D) cell cultures, use and kit thereof are provided. The device includes outer and inner walls, the inner wall defining a volume and is located within the outer wall, forming a cavity therebetween. It also includes a base configured to position a cell culture sample below the volume within the inner wall and one or more partitions connected to the base, connecting the inner wall to the outer wall and segmenting the cavity into a plurality of voids. Openings in surfaces forming the voids allow fluid flow from a first void, below the volume within the inner wall to a second void, the fluid flow configured to interact with the cell culture sample when flowing through a sample region within the inner wall. The sample region has a depth defined by a height of the inner wall greater than a length defined by an inner distance across the volume within the inner wall.

The utility model discloses a microcarrier structure for 3D (three-dimensional) cell culture, belongs to the technical field of cell culture, and solves the problem that the center of a cell ball still lacks a growth microenvironment while the three-dimensional growth of the cell ball is realized by the existing cell culture device. The device comprises a sphere-like polyhedron with a hollow interior, the interior of the sphere-like polyhedron is filled with a culture medium, the plane of the sphere-like polyhedron comprises a solid plane, and a plurality of round holes penetrating through the solid plane are uniformly formed in the solid plane; the plane of the sphere-like polyhedron comprises at least one group of two cavity planes. The sphere-like polyhedron disclosed by the utility model is similar to a sphere and is beneficial to rapid and stable adhesion and growth of cells, and meanwhile, a culture medium can be filled in the hollow interior of the sphere-like polyhedron so as to simulate a central microenvironment when a cell cluster or a cell sphere is formed, so that the exchange of nutrition and oxygen of internal cells and the excretion of wastes are met; and the necrosis of cells in a cell cluster or a cell ball during 3D cell culture is avoided.

Methods and compositions for modulating hydrogel gelation and mechanics for 3D cell culture are provided. Hydrogels have a variety of benefits for cell and tissue culture, including without limitation homogeneous distribution of materials and cells at the initiation of the 3D cell culture, disruption of network formation and disassociation of the 3D cell culture for cell retrieval, etc. The method for modulating the stiffness of a hydrogel solution comprises contacting a hydrogel solution with an effective amount of a competitor agent sufficient to modulate the stiffness of the hydrogel solution. In some embodiments, the methods further comprise contacting the hydrogel solution with a catalyst agent wherein the catalyst agent increases the forward and reverse reaction rate of reversible covalent bond formation that occurs in the hydrogel solution.

The application discloses an immortalized human hair papilla cell line and application thereof, and belongs to the technical field of immortalized cells, and particularly relates to a preparation method of an immortalized human hair papilla cell line; the method comprises the following steps: infecting human hair papilla cells by using an SV40 LT overexpression vector combined with a dyeing aid, treating the human hair papilla cells by using a puromycin screening solution, picking single clone cells by using a limited dilution method, and expanding the culture of the single clone cells to obtain the immortalized human hair papilla cell line; the dyeing aid comprises polybrene; compared with human hair papilla cells, the immortalized human hair papilla cell line prepared by the preparation method has higher cell activity and cell proliferation capacity, and can form 3D cell balls when cultured in vitro in a 3D mode, and the immortalized human hair papilla cell line retains the ability of inducing hair follicle formation, and can be applied to the fields of hair follicle tissue engineering, 3D cell culture or hair follicle organoid construction and the like.

The invention provides a human tonsil / adenoid epithelial cell culture method and a culture medium, and belongs to the technical field of biomedicine. The culture method comprises the following steps: separating epithelial tissues from in-vitro human tonsil / adenoid tissues; digesting epithelial tissues to obtain cell precipitates; lymphocytes are removed in a magnetic bead sorting mode, and primary human tonsil / adenoid epithelial cells are obtained through separation; carrying out amplification and differentiation culture by adopting a 3D cell culture medium, generating organoids at the upper part and generating adherent cells at the bottom; and carrying out enzymolysis on the adherent cells, and separating to obtain the human tonsil / adenoid epithelial cells. The culture method provided by the invention is relatively high in success rate and simple in steps, the human tonsil / adenoid epithelial cells obtained by culture are high in purity and can be continuously proliferated, and long-term in-vitro culture of the tonsil / adenoid epithelial cells is successfully realized.

The application belongs to the technical field of biology and specifically relates to a kind of mesenchymal stem cell aggregate jointly enabled by matrix and cytokine and application, the aggregate is by mesenchymal stem cell in the medium added cell culture matrix and IGF-1 is cultivated and obtains pretreated mesenchymal stem cell, continue in 3D cell culture system and obtain by generation culture, the calcium adhesion cell culture matrix is prepared by 1-3 parts hE-cad-Fc and 1-3 parts hN-cad-Fc;The aggregate structure obtained is complete, boundary is clear, high expression intracellular endogenous calcium adhesion and phosphorylated IGF-1 receptor, the curative effect of complex etiology, refractory, recurrent immune disorder diseases is significantly improved, such as systemic sclerosis, xerophthalmia and systemic lupus erythematosus.