18 results about "3D cell culture" patented technology

A set of 3D cell culture colloids and a method for 3D cell culture therewith are provided. [Solution] The 3D cell culture colloid set includes gel material A, buffer solution C, and buffer solution D. The 3D cell culture method includes the steps of adding cells to a mixture containing gel material A, allowing it to react at low temperature to obtain a colloid containing the cells, adding buffer solution C to the colloid to crosslink it, removing buffer solution C and adding a growth medium, and culturing the cells until spheroids are formed within the colloid. The present invention also further dissolves the colloid with buffer solution D, and extracts and analyzes the cells after culturing. Therefore, the 3D cell culture colloid set of the present invention is easy to use and can be used for 3D cell culture of a variety of cell lines.

A microcavity bioreactor is provided that allows cell growth and cell differentiation in the same vessel, such that higher order three-dimensional cell cultures such as organoids can be generated in a singular vessel. The microcavity bioreactor may be part of a microcavity bioreactor system that allows for perfusion based cell culture and perfusion based cell harvesting. The microcavity bioreactor includes at least one, and preferably at least two, fluid distributor structures in the housing vessel of the microcavity bioreactor.

A gellable material for encapsulating a cellular aggregate in a well plate is provided. The cellular aggregate, before or after encapsulation, can be organoid or a spheroid, and typically, the cellular aggregate, once encapsulated, has substantially no movement during media exchange, handling of the well plate or tilting of the well plate. Methods for producing encapsulated cellular aggregates, and uses thereof are also provided.

The present invention relates to a pharmaceutical composition for preventing or treating cartilage disease or inflammatory joint disease and a method for preparing a chondrospheroid having chondrocyte differentiation ability. The present invention provides a pharmaceutical composition comprising a stem cell-derived spheroid as an active ingredient for preventing or treating cartilage disease or inflammatory joint disease, and a method for preparing a chondrospheroid having chondrocyte differentiation ability, the method comprising the steps of: Inoculating stem cells into a three-dimensional (3D) cell culture vessel made of a plastic material; treating the stem cells inoculated into the 3D cell culture vessel with a chondrocyte differentiation-inducing factor; culturing the stem cells treated with the chondrocyte differentiation-inducing factor to form chondrospheroids; and separating the formed chondrospheroids.

The invention provides a chip model for detecting whether candidate drugs can cause cytokine storm, and belongs to the technical field of biomedicine. The chip model comprises a culture plate, the culture plate comprises a plurality of culture units, and each culture unit comprises a culture solution channel and a plurality of cell growth holes communicated with the culture solution channel; the bottom of the cell growth hole is lower than the bottom of the culture solution channel to form a growth hole concave part. According to the chip model, medicine combined immune cell killing can be carried out while 3D cell culture is realized, and whether side reaction is generated or not is detected in subsequent experiments. According to the application, higher-throughput culture and detection can be carried out, the experimental purpose of detecting and evaluating a plurality of anti-tumor drugs on the same chip is achieved, and drug safety evaluation can be carried out through components of a culture solution while drug effect evaluation is carried out.

The application belongs to the technical field of biology and specifically relates to a kind of mesenchymal stem cell aggregate jointly enabled by matrix and cytokine and application, the aggregate is by mesenchymal stem cell in the medium added cell culture matrix and IGF-1 is cultivated and obtains pretreated mesenchymal stem cell, continue in 3D cell culture system and obtain by generation culture, the calcium adhesion cell culture matrix is prepared by 1-3 parts hE-cad-Fc and 1-3 parts hN-cad-Fc;The aggregate structure obtained is complete, boundary is clear, high expression intracellular endogenous calcium adhesion and phosphorylated IGF-1 receptor, the curative effect of complex etiology, refractory, recurrent immune disorder diseases is significantly improved, such as systemic sclerosis, xerophthalmia and systemic lupus erythematosus.

The utility model belongs to the technical field of 3D cell culture, and discloses a 3D cell culture plate which comprises a plate body, a plurality of reaction tanks are arranged on the plate body, and the plurality of reaction tanks are distributed in an adjacent array; a sample adding hole and a plurality of reaction holes are formed in the bottom of each reaction tank, and the plurality of reaction holes are arranged around the circumferential direction of the sample adding hole. A culture medium can be sucked, discarded and added through the sample adding hole formed in the middle, so that the culture medium is collected from the sample adding hole to be sucked and discarded in the suction and discarding culture process; and in the process of adding the culture medium, the culture medium is diffused from the sample adding hole to the surrounding reaction holes, so that the interference on cells in the processes of absorbing and abandoning the culture medium and adding the culture medium is reduced, the efficiency is improved, the data stability is improved, and meanwhile, the plurality of reaction tanks can also meet the simultaneous culture of a plurality of cell modules.

The invention relates to the technical field of biology, in particular to a novel phenylboronic acid modified chitosan microcarrier, a preparation method and application, and the phenylboronic acid modified chitosan microcarrier is obtained by mixing 4-formyl phenylboronic acid and medium-viscosity chitosan with gelatin and crosslinking. The particle size of the microcarrier is about 100 [mu] m, and scanning electron microscope analysis shows that the microcarrier shows a clear spherical porous form, has uniform pore shape and uniform pore size, and can provide a three-dimensional environment for cell growth. The prepared porous material has moderate hardness, elasticity and pore wall, is more beneficial to growth of more cells and transmission of nutrient substances, has higher stability, is more suitable for cell culture, shows excellent biological safety and biocompatibility, and is more suitable for a 3D cell culture system.

The invention relates to a Fibroblast Growth Factor 2 (FGF-2) polypeptides with improved stability while maintaining biological activity compared to the wild-type of FGF-2. The invention provides the process for the preparation, stabilisation and the use thereof in biotechnological research and industrial applications, the pharmaceutical industry, cosmetics, clean meat industry, generation of organoids, 3D cell culture models, in combination with physical modalities and other related applications.

The application discloses a 3D hepatotoxicity test monodisperse microgel preparation method and system and medium, and belongs to the technical field of 3D cell culture and drug screening. The system comprises a preparation module, a statistical analysis module, a material processing module and a control system; the method comprises the following steps: collecting historical data to train an adaptive neural network, and establishing a mapping relationship between control parameters and microgel quality indexes; taking the neural network as a prediction model, and solving optimal control input through a model predictive controller; executing microgel preparation; collecting images in real time to obtain actual particle sizes and variation coefficients and feeding back; using new data for incremental updating of the neural network; and iteratively forming a closed-loop optimization process. The application realizes multi-parameter adaptive optimization through an artificial intelligence algorithm, solves the problem of artificial groping of different hydrogel material parameters, improves the monodispersity and consistency of the microgel, and realizes full-process automation.

A 3D perfusion bioreactor system and method for more accurate modeling of in vivo environmental conditions and cellular behaviors. In some embodiments, the bioreactor includes a cavity for containing culture media and cells. A plurality of capillary tubes cross the cavity. Artery capillary tubes may transport culture media received from an inlet of the bioreactor, across the cavity, and into a return compartment. Vein capillary tubes may transport culture media from the return compartment, across the cavity, and to an outlet of the bioreactor. Metabolites may diffuse from the capillary tubes into the cavity for cellular consumption, and metabolic waste may diffuse from the cavity into the capillary tubes for removal from the bioreactor. In some embodiments, culture media discharged from the outlet may be processed via a waste treatment device and returned to the inlet.

The application discloses a kind of human mesenchymal stem cell 3D culture and amplification method, belong to stem cell culture amplification technical field.The application is prepared 3D cell culture matrix by structure design and component optimization, realize the accurate simulation of stem cell in-vivo microenvironment.The matrix is modified sodium alginate by amino hexene acid and is compounded with methacryl gelatin, and is crosslinked by lithium chloride and calcium chloride, and is synergistically formed by photocrosslinking, and the three-dimensional network has uniform pore size and excellent connectivity.The application significantly improves cell adhesion rate and proliferation efficiency, effectively maintains the biological characteristics of hMSC such as immune regulation and differentiation, the degradation rate of the matrix is dynamically matched with the cell proliferation cycle, the material has excellent biocompatibility and the degradation product is non-toxic, which solves the defects of traditional 2D culture and existing 3D technology, and provides an efficient and safe technical solution for hMSC scale-up and clinical translation.

The invention provides a non-spherical porous foam material and a preparation method and application thereof, the preparation method comprises the following steps: (1) mixing a polymerizable monomer, a cross-linking agent, an emulsifier, a stabilizer and an initiator to obtain an oil phase; (2) mixing deionized water, electrolyte and an initiator to obtain a water phase; (3) preparing a rodlike high internal phase emulsion or an ellipsoidal high internal phase emulsion from the oil phase and the water phase by adopting microfluidic equipment; and (4) carrying out ultraviolet precuring on the rodlike high-internal-phase emulsion or the ellipsoidal high-internal-phase emulsion, and then carrying out thermocuring and post-treatment to respectively obtain a rodlike porous foam material or an ellipsoidal porous foam material, namely the non-spherical porous foam material. The invention provides an accurate preparation scheme of the non-spherical HIPE based on a microfluidic technology, controllable preparation of the non-spherical HIPE is realized, and the non-spherical porous foam material formed after curing can provide a high-performance carrier for 3D cell culture in the fields of regeneration, repair and the like.

A well plate and a 3D cell culture plate are described. The well plate having a repeating pattern. The wells of the well plate is continuously provided with a space part in between a main well to be injected with culture solution and a sub well including a recessed part where the cells are cultured. The main well of the well plate is connected to the space part with forming a step, and the sub well is connected to the part with forming an inclined surface.

This invention provides compositions of polyvinylpyrrolidone (PVP-based) synthetic hydrogels and methods of using them. The PVP-based hydrogels comprise thiol-functionalized PVP, multi-arm polyethylene glycol sulfone, RGD peptides, and VPM peptides. The PVP-based synthetic hydrogels are suitable for three-dimensional cell culture and are readily soluble. These PVP-based synthetic hydrogels possess at least the following advantages: their mechanical properties are comparable to non-synthetic Matrigel. ® Similar, but with a more specific definition.

Compositions of chitosan-based synthetic hydrogels and methods of using the same are provided. The chitosan-based hydrogel is prepared from functionalized chitosan, multi-arm polyethylene glycol vinyl sulfone, RGD peptide and VPM peptide. The chitosan-based synthetic hydrogel can be used for three-dimensional cell culture and can be dissolved. These chitosan-based synthetic hydrogels have at least the following advantages: their mechanical properties are similar to those of non-synthetic Matrigol, but the definition is clearer.

Fluid supply module for a cell culture system for 3D in vitro perfusion cell culture, wherein the fluid supply module comprises a fluid drive mechanism configured to move the fluid over a cell culture area of ​​a cell culture module, and wherein the fluid supply module is configured to be assembled with the cell culture module to form a cell culture system for 3D in vitro perfusion cell culture that is fluidically isolated from the external environment.

The invention discloses a 3D hepatotoxicity test monodisperse microgel preparation method, a 3D hepatotoxicity test monodisperse microgel preparation system and a medium, and belongs to the technical field of 3D cell culture and drug screening. The system comprises a preparation module, a statistical analysis module, a material processing module and a control system, the method comprises the following steps: collecting historical data to train an adaptive neural network, and establishing a mapping relation between control parameters and microgel quality indexes; a neural network is used as a prediction model, and optimal control input is solved through a model prediction controller; performing microgel preparation; acquiring images in real time to obtain and feed back actual particle sizes and variation coefficients; applying the new data to incremental updating of the neural network; and performing iteration to form a closed-loop optimization process. According to the method, multi-parameter self-adaptive optimization is realized through an artificial intelligence algorithm, the problem of manual exploration of parameters of different hydrogel materials is solved, the monodispersity and consistency of microgel are improved, and full-process automation is realized.