7 results about "3D cell culture" patented technology

A set of 3D cell culture colloids and a method for 3D cell culture therewith are provided. [Solution] The 3D cell culture colloid set includes gel material A, buffer solution C, and buffer solution D. The 3D cell culture method includes the steps of adding cells to a mixture containing gel material A, allowing it to react at low temperature to obtain a colloid containing the cells, adding buffer solution C to the colloid to crosslink it, removing buffer solution C and adding a growth medium, and culturing the cells until spheroids are formed within the colloid. The present invention also further dissolves the colloid with buffer solution D, and extracts and analyzes the cells after culturing. Therefore, the 3D cell culture colloid set of the present invention is easy to use and can be used for 3D cell culture of a variety of cell lines.

A microcavity bioreactor is provided that allows cell growth and cell differentiation in the same vessel, such that higher order three-dimensional cell cultures such as organoids can be generated in a singular vessel. The microcavity bioreactor may be part of a microcavity bioreactor system that allows for perfusion based cell culture and perfusion based cell harvesting. The microcavity bioreactor includes at least one, and preferably at least two, fluid distributor structures in the housing vessel of the microcavity bioreactor.

The present invention relates to a pharmaceutical composition for preventing or treating cartilage disease or inflammatory joint disease and a method for preparing a chondrospheroid having chondrocyte differentiation ability. The present invention provides a pharmaceutical composition comprising a stem cell-derived spheroid as an active ingredient for preventing or treating cartilage disease or inflammatory joint disease, and a method for preparing a chondrospheroid having chondrocyte differentiation ability, the method comprising the steps of: Inoculating stem cells into a three-dimensional (3D) cell culture vessel made of a plastic material; treating the stem cells inoculated into the 3D cell culture vessel with a chondrocyte differentiation-inducing factor; culturing the stem cells treated with the chondrocyte differentiation-inducing factor to form chondrospheroids; and separating the formed chondrospheroids.

The application discloses a 3D hepatotoxicity test monodisperse microgel preparation method and system and medium, and belongs to the technical field of 3D cell culture and drug screening. The system comprises a preparation module, a statistical analysis module, a material processing module and a control system; the method comprises the following steps: collecting historical data to train an adaptive neural network, and establishing a mapping relationship between control parameters and microgel quality indexes; taking the neural network as a prediction model, and solving optimal control input through a model predictive controller; executing microgel preparation; collecting images in real time to obtain actual particle sizes and variation coefficients and feeding back; using new data for incremental updating of the neural network; and iteratively forming a closed-loop optimization process. The application realizes multi-parameter adaptive optimization through an artificial intelligence algorithm, solves the problem of artificial groping of different hydrogel material parameters, improves the monodispersity and consistency of the microgel, and realizes full-process automation.

The application discloses a kind of human mesenchymal stem cell 3D culture and amplification method, belong to stem cell culture amplification technical field.The application is prepared 3D cell culture matrix by structure design and component optimization, realize the accurate simulation of stem cell in-vivo microenvironment.The matrix is modified sodium alginate by amino hexene acid and is compounded with methacryl gelatin, and is crosslinked by lithium chloride and calcium chloride, and is synergistically formed by photocrosslinking, and the three-dimensional network has uniform pore size and excellent connectivity.The application significantly improves cell adhesion rate and proliferation efficiency, effectively maintains the biological characteristics of hMSC such as immune regulation and differentiation, the degradation rate of the matrix is dynamically matched with the cell proliferation cycle, the material has excellent biocompatibility and the degradation product is non-toxic, which solves the defects of traditional 2D culture and existing 3D technology, and provides an efficient and safe technical solution for hMSC scale-up and clinical translation.

This invention provides compositions of polyvinylpyrrolidone (PVP-based) synthetic hydrogels and methods of using them. The PVP-based hydrogels comprise thiol-functionalized PVP, multi-arm polyethylene glycol sulfone, RGD peptides, and VPM peptides. The PVP-based synthetic hydrogels are suitable for three-dimensional cell culture and are readily soluble. These PVP-based synthetic hydrogels possess at least the following advantages: their mechanical properties are comparable to non-synthetic Matrigel. ® Similar, but with a more specific definition.

The invention discloses a 3D hepatotoxicity test monodisperse microgel preparation method, a 3D hepatotoxicity test monodisperse microgel preparation system and a medium, and belongs to the technical field of 3D cell culture and drug screening. The system comprises a preparation module, a statistical analysis module, a material processing module and a control system, the method comprises the following steps: collecting historical data to train an adaptive neural network, and establishing a mapping relation between control parameters and microgel quality indexes; a neural network is used as a prediction model, and optimal control input is solved through a model prediction controller; performing microgel preparation; acquiring images in real time to obtain and feed back actual particle sizes and variation coefficients; applying the new data to incremental updating of the neural network; and performing iteration to form a closed-loop optimization process. According to the method, multi-parameter self-adaptive optimization is realized through an artificial intelligence algorithm, the problem of manual exploration of parameters of different hydrogel materials is solved, the monodispersity and consistency of microgel are improved, and full-process automation is realized.