4results about How to "Reduce cell viability" patented technology

The invention discloses an MMP2 gene promoter region SNP (Single Nucleotide Polymorphism) site and application thereof in pig breeding. According to the invention, an MMP2 gene promoter region is taken as a research object, and the relevance between the MMP2 gene promoter region and sow sexual maturity is researched by adopting a molecular and cell biology method, so that the situation that g.30088284G > A site and g.30088004C > T site are obviously related to the group maturity day age of Dumet black pigs is found; then different vectors are transfected, and different influences of different genotypes of the sites on the activity of the IRS1 gene promoter are studied. Further, sow ovarian granular cells are used as experimental materials, experimental technologies such as transcriptome sequencing, a total iron colorimetric method and a malondialdehyde colorimetric method are adopted, the influence of the MMP2 gene on the transcriptome level and ferroptosis of the sow ovarian granular cells is researched, and it is found that cell transcriptome differential genes influenced by the MMP2 gene are mainly enriched to signal channels such as cell differentiation; and ferroptosis of cells is promoted.

The invention discloses a pharmaceutical composition for inhibiting tumor drug resistance and application thereof. The pharmaceutical composition comprises an exosome inhibitor, nintedanib and gemcitabine, the exosome inhibitor, the nintedanib and the gemcitabine are used in a combined manner, so that the cell viability of the CTS can be remarkably reduced, and the proportion of apoptotic cells in the CTS can be remarkably increased; the pharmaceutical composition interferes with the mutual transformation process between normal fibroblasts and CAFs, and inhibits secretion and release of EVs and reduces formation of new CAFs while restoring the CAFs existing in TME to normal fibroblasts, thereby successfully reversing the drug resistance of tumors.

The invention belongs to the technical field of traditional Chinese medicines, particularly discloses application of an Anluo chemical fiber pill in preparation of medicines for inhibiting liver cancer cell proliferation and promoting liver cancer cell apoptosis, and discloses that the Anluo chemical fiber pill can reduce the cell survival rate of human liver cancer cells Hep G2, Hep 3B and SK-Hep-1 and has time and dose dependence; the Anluo chemical fiber pills can inhibit proliferation, invasion and migration of Hep G2 and Hep 3B cells and induce apoptosis of the Hep G2 and Hep 3B cells at the same time, and the apoptosis mostly occurs at the early stage of cell apoptosis; the Anluo chemical fiber pill can promote apoptosis of Hep G2 and Hep 3B cells by increasing the content of ROS in the Hep G2 and Hep 3B cells, and has dependence on time and dosage, so that the Anluo chemical fiber pill can be used for preparing the medicine for inhibiting liver cancer cell proliferation and promoting apoptosis of liver cancer cells.

This invention discloses the application of a polysaccharide coating membrane in the preparation of cell culture products, belonging to the field of cell culture food technology. Specifically, after cells are seeded onto a scaffold, a polysaccharide coating membrane is formed around it. This can alleviate the phenomenon of cells detaching from the scaffold during culture, thereby ensuring the effective proliferation and differentiation of muscle stem cells and / or adipose stem cells on the scaffold, providing a new solution for the efficient and standardized production of cell culture products.