Targeted non-viral DNA insertions

a dna insertion and non-viral technology, applied in the field of targeted non-viral dna insertions, can solve the problems of protein to be knocked out of the frame, many applications cannot be served by this method, and mass cell death, so as to reduce the loss of cell viability and reduce the off-target
US20200362355A1Inactive Publication Date: 2020-11-19RGT UNIV OF CALIFORNIA
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
RGT UNIV OF CALIFORNIA
Publication Date
2020-11-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

Provided herein are methods and compositions for editing the genome of a cell. In some embodiments, a nucleotide sequence of at least 200 nucleotides in length is inserted into a target region in the genome of a cell.
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Description

PRIOR RELATED APPLICATIONS

[0001] This application claims the benefit of U.S. Provisional Application No. 62 / 520,117 filed on Jun. 15, 2017 and U.S. Provisional Application No. 62 / 552,180 filed on Aug. 30, 2017, both of which are hereby incorporated by reference in their entireties.STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSORED RESEARCH AND DEVELOPMENT

[0002] This invention was made with government support under grant no. P50 GM082250 awarded by the National Institutes of Health. The government has certain rights in the invention.BACKGROUND OF THE INVENTION

[0003] The ability to introduce small mutations (indels) at targeted sites in the genome of cells by electroporating a Cas9-gRNA complex (RNP) into the cells has been developed. However, since these mutations are random and introduced by non-homologous end joining, they can cause a protein to be knocked out of frame (Schumann et al. PNAS 112(33): 10437-10442 (2015)). Other methods have been developed to introduce a...

Claims

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