554 results about "Sense strand" patented technology

Methods and compositions for performing RNA interference with decreased off-target effects are provided The methods and compositions permit effective and efficient applications of RNA interference to applications such as diagnostics and therapeutics through the use of modifications to the siRNA. Uniquely modified siRNAs have been developed that reduce off-target effects incurred in gene-silencing. The modifications comprise 2′-O-alkyl or mismatch modification(s) at specific positions on the sense and / or antisense strands.

This patent application discloses siRNA sequences against the constant region of the influenza virus nucleoprotein gene comprising:Sense strand:5′ UGAAGGAUCUUAUUUCUUCdTdT 3′(SEQ ID NO: 1)Anti sense strand:3′ dTdTACUUCCUAGAAUAAAGAAG 5′(SEQ ID NO: 2)Sense strand:5′ UGAAGGAUCUUAUUUCUUCGGdTdT 3′(SEQ ID NO: 3)Anti sense strand:3′ dTdTACUUCCUAGAAUAAAGAAGCC 5′(SEQ ID NO: 4)Sense strand:5′ GGAUCUUAUUUCUUCGGAGACdTdT 3′(SEQ ID NO: 5)Anti sense strand:3′ dTdTCCUAGAAUAAAGAAGCCUCUG 5′(SEQ ID NO: 6)said sequences being inhibitory against influenza virus in animals including humans. The invention further includes one or more of said siRNA sequences in the form of an aqueous suspension suitable for nasal inhalation. Still further, the invention includes one or more of said siRNA sequences in the form of a plasmid expressing intracellularly in animals including humans. In another aspect, the invention includes one or more of said siRNA sequences in the form of an AAV vector adapted to express intercellularly and establish a permanent inhibitory effect against influenza virus by integrating to the cellular chromosome of animals including humans. The invention also includes the administration to an animal including humans, in a therapeutically effective amount, at least one of said siRNA sequences.

The present disclosure provides compositions and methods for accurately detecting mutations by uniquely tagging double stranded nucleic acid molecules with dual cyphers such that sequence data obtained from a sense strand can be linked to sequence data obtained from an anti-sense strand when sequenced, for example, by massively parallel sequencing methods.