15 results about "Coding strand" patented technology

Disclosed are methods of making a genetically cell that expressed FOXP3 and methods of treatment. In some embodiments, the method can providing a first nucleotide sequence, wherein the first nucleotide sequence comprises a coding strand, the coding strand comprising one or more regulatory elements and a FOXP3 gene or portion thereof providing a nuclease and performing a gene editing process on the first nucleotide sequence, which edits said one or more regulatory elements, and optionally edits the FOXP3 gene or portion thereof. Methods of treating a subject suffering from an autoimmune disease and subjects suffering the effects of organ transplantation are also provided.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for identifying the fiber length of sea island cotton as well as an identification method and application of the SNP molecular marker. The invention belongs to the technical field of biology, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker for identifying the fiber length of sea island cotton as well as an identification method and application of the SNP molecular marker. The invention provides a method for identifying or assisting in identifying the length of sea island cotton fibers, which comprises the following steps: detecting the genotype of an SNP (Single Nucleotide Polymorphism) site in a sea island cotton genome to be detected, and identifying or assisting in identifying the length of the sea island cotton fibers according to the genotype, the SNP site is an SNP site on a sea island cotton D10 chromosome, and the nucleotide type in a coding chain is C or T; the nucleotide is the 100th nucleotide of the sequence 1 in the sequence table. The SNP molecular marker obviously related to the sea island cotton fiber length can be used for assisting in breeding a sea island cotton variety with excellent fiber quality, and a breakthrough cotton variety with longer fiber and better quality can be bred in an accelerated manner.

The invention belongs to the technical field of information storage, and particularly provides a self-adaptive fluorescence signal reading method based on a convolutional neural network, which comprises the following steps: S1, hybridizing a DNA decoding chain with a coding chain in a micro pool of a storage substrate to obtain a primary image group of the hybridized storage substrate, and marking a fluorescence probe at the tail end of the decoding chain; s2, segmenting the primary image according to a micro-pool unit, extracting a plurality of sub-images, and performing data enhancement to obtain a secondary image; s3, marking the secondary image; s4, dividing the data set into a training set and a verification set; s5, inputting a training set into the CNN model for training, and assisting dynamic adjustment and optimization of hyper-parameters; and S6, applying the trained CNN model to test a secondary image, and outputting a fluorescence recognition result. According to the method, the judgment standard can be automatically learned according to a large number of training samples, the fluorescence signal can be accurately, stably and efficiently read, manual threshold value presetting is not needed, the influence of interference factors is small, and the robustness and accuracy of DNA information storage can be effectively improved.

The invention discloses a DNA encoding method and device and a DNA decoding method and device, and belongs to the technical field of information storage and security. The DNA encoding method comprises the steps that target information is converted into computer codes; converting the computer code into a DNA coding chain or a DNA coding chain combination; and cross-linking the DNA coding chain and / or the DNA coding chain combination in a storage micro-pool on a DNA information storage medium so as to realize the storage of the target information. According to the method, the information storage process is completely independent of DNA synthesis and DNA sequencing technologies, and the method has the advantages of high parallelism, short access time, good encryption, low cost, strong expansibility and the like.

A sender may provide, to a recipient, encrypted DNA material comprising a set of DNA fragments. The sender may provide, to the recipient, a decryption key including instructions for ligating the set of DNA fragments to construct an encoded DNA strand having a nucleotide sequence that encodes a message.

The present invention discloses a multi-level directory DNA storage encoding and decoding method based on modulation technology, comprising the following steps: S100: generating a modulation code table for primer sequences and file data sequences, constructing a primer sequence data set and a file absolute path set; S200: modulating the binary file to be stored into a DNA sequence, synthesizing and storing it in vitro; S300: selecting target primers, amplifying DNA molecules under the target logical disk from the synthesis pool, and sequencing them; S400: calculating the absolute file path of each read length of the sequenced data based on the observed modulation sequence, and grouping the sequenced data based on this; S500: generating a modulation sequence for decoding each grouped data based on the encoded DNA chain length and the absolute file path; and using this modulation sequence to decode the data according to the modulation DNA storage decoding algorithm. The present invention designs an addressing sequence based on modulation technology and proposes a multi-level directory DNA storage encoding and decoding method, which can realize an efficient and flexible DNA storage file management method.

The present invention is concerned with linear double stranded DNA, which is coupled to a single support or a tag at the 3′ end of its non-coding strand and methods for producing said linear double stranded DNA. The present invention further relates to the use of said linear double stranded DNA in an RNA in vitro transcription reaction and also to a method for producing RNA in vitro. The present invention also relates to a bioreactor for RNA in vitro transcription.

This invention discloses a method and system for evaluating DNA storage sequencing depth based on channel simulation, addressing the problems of inaccurate predictions and limited guidance in existing uniform distribution models. The method includes: when sequencing data is available, fitting real data to obtain log-normal distribution parameters μ and σ; when sequencing data is unavailable, obtaining μ and σ through simulation modeling based on experimental parameters; and combining these parameters to calculate the decoding ratio of the coding strand in the noiseless channel and the sequencing depth boundary in the noisy channel. The system includes input, storage channel modeling, sequencing depth calculation, and output modules. The model of this invention closely reflects reality, provides accurate predictions, reduces DNA storage and retrieval costs, improves the success rate of single-sequencing decoding, and is easy to use, facilitating the practical application of the technology.

The invention provides a high-fidelity time sequence fluorescent DNA bar code and application thereof. The high-fidelity time sequence fluorescent DNA bar code comprises a coding chain and a reading chain, the coding chain is formed by connecting three sections of coding areas and four sections of positioning areas in series; the reading chain comprises a reading chain 1, a reading chain 2 and a reading chain 3; after the reading chain 1 is specifically combined with the coding chain, a signal carried on the reading chain 1 is generated; the reading chain 2 replaces and releases the reading chain 1 combined on the bar code through a toehold-mediated chain replacement reaction, and generates a signal carried by the reading chain 2; the reading chain 3 replaces and releases the reading chain 2 combined on the bar code through a toehold-mediated chain replacement reaction, and generates a signal carried by the reading chain 3; therefore, a time sequence change signal which can be detected is generated and is used for identifying the target molecule. The DNA bar code can exponentially expand the coding capability of the DNA bar code, and in-situ multiple imaging and analysis of various proteins, nucleic acids or polysaccharides can be easily realized.

The present invention is concerned with linear double stranded DNA, which is coupled to a single support or a tag at the 3′ end of its non-coding strand and methods for producing said linear double stranded DNA. The present invention further relates to the use of said linear double stranded DNA in an RNA in vitro transcription reaction and also to a method for producing RNA in vitro. The present invention also relates to a bioreactor for RNA in vitro transcription.

This application discloses a gene, a recombinant vector, an engineered bacterium, and their applications. A limonene synthase (LS) gene is provided, wherein the limonene synthase (LS) gene is selected from the following DNA fragments: a1) or a2); a1) comprises a DNA fragment whose coding sequence is the DNA fragment represented by SEQ ID NO. 1; and a2) comprises a DNA fragment that is 90% or greater identical to the DNA fragment defined in a1) and encodes a protein having the same function. The limonene synthase (LS) gene is optimized and can be successfully expressed in host cells.

The invention discloses a DNA short chain hybridization fluorescence signal threshold calculation and discrimination method, and relates to the technical field of DNA storage, and the method comprises the following steps: obtaining fluorescence signal intensity data of DNA coding chains in a plurality of micro-pools to form a data set; calculating at least three statistics according to the data set, wherein the statistics comprise a strong signal mean value and a weak signal mean value; calculating a dynamic threshold based on the statistics; comparing the fluorescence signal intensity data with the dynamic threshold value, and judging the existence state of the DNA coding chain in each micro-pool; and generating binary information according to the existence state combination. Based on the obtained fluorescence intensity data, a reasonable fluorescence threshold value is dynamically set, and effective signals and error interference signals can be effectively distinguished; and on the basis of actual data distribution characteristics, a fluorescence threshold is dynamically calculated, and whether a signal exists or not is scientifically judged, so that DNA information is accurately and reliably read under various complex and abnormal environment conditions.

The invention belongs to the technical field of biology. More specifically, the invention provides a gene of a rice virus coding protein. The invention discloses the existence of extra small protein ORF8 on a rice stripe mosaic virus (RSMV) coding chain for the first time, and researches show that a rice plant overexpressed with the ORF8 gene shows significantly reduced virus accumulation in the middle and later periods of RSMV infection, which indicates that overexpression of the ORF8 gene can effectively inhibit replication and diffusion of the RSMV in rice, so that the rice plant can effectively inhibit the rice from being infected by the RSMV. Therefore, the harm of viruses to plants is obviously reduced. The invention provides a method for constructing an anti-RSMV plant variety by using the ORF8 gene, and the resistance of the plant to the RSMV virus is enhanced, so that the prevention and treatment of the rice stripe mosaic disease are realized, the application prospect and value are very good, and an important technical support is provided for sustainable development of agricultural production.

A method for screening αβ-TCR mutants, the method comprising the construction of a phagemid. In the construction, a gene encoding an A1 polypeptide and a gene encoding B1 polypeptide are inserted into the 3′ ends of genes encoding the constant regions of the α chain and the β chain of TCR respectively, and a ribosome binding site and a molecular chaperone gene are integrated into a phage vector, wherein the amino acid sequence of the A1 polypeptide is as shown in SEQ ID NO: 1 or is represented by the variant of the A1 polypeptide; the amino acid sequence of the B1 polypeptide is as shown in SEQ ID NO: 2 or is represented by the variant of the B1 polypeptide; and the variants at least retain the function of the polypeptides before mutation. The A1 peptide and the B1 peptide are used for promoting the correct pairing and folding of the αβ heterodimer TCR, so that the efficiency of displaying the correctly folded TCR on the surface of a phage is improved. The method can efficiently complete the screening of αβ heterodimer TCR mutants.

The application belongs to the technical field of life science, and particularly relates to a method for preparing a hyperlipidemia mouse model. The method uses BE4max to target edit an antisense strand of the 6th exon of a mouse Ldlr gene, introduces a stop codon on the coding strand of the Ldlr gene by editing C on the target site into T, causes the Ldlr protein expression to be lost, and obtains the hyperlipidemia mouse model. The application also provides a specific sgRNA used in the method, and the sequence of the sgRNA is shown as SEQ ID NO:1. The method has high construction efficiency, short cycle, and controllable editing accuracy, the positive rate of F0 generation mice reaches 100%, and the editing efficiency of part of the mice is more than 90%, and after being induced by high-fat feed, the mice can produce significant hyperlipidemia and atherosclerosis phenotype, and can be widely applied to mechanism research and drug screening of hyperlipidemia and atherosclerosis.