51 results about "Coding strand" patented technology

A method of screening a candidate compound for susceptibility to biliary excretion by a hepatocyte transport protein. The method includes the steps of providing a culture of hepatocytes comprising a transport protein, the culture having at least one bile canaliculus; exposing a candidate compound to the culture; and determining an amount of candidate compound in the at least one bile canaliculus, the amount of candidate compound in the at least one bile canaliculus indicating the susceptibility of the candidate compound to biliary excretion by the transport protein. In some embodiments determining the amount of candidate compound in the bile canaliculus comprises inhibiting expression of the transport protein, measuring the amount of candidate compound in the bile canaliculus and comparing amounts of compound in the canalicules with and without inhibition of the transport protein. A difference in the amount of candidate compound in the canaliculus indicates susceptibility of the candidate compound to biliary excretion by the transport protein. In one embodiment, expression of the transport protein is inhibited through introduction of a RNA having a sequence corresponding to a coding strand of the gene encoding the transport protein into the hepatocyte. Optionally, the culture of hepatocytes is a long-term culture in a sandwich configuration. The method is particularly applicable to the screening of multiple candidate compounds in a single effort.

Disclosed are methods of making a genetically cell that expressed FOXP3 and methods of treatment. In some embodiments, the method can providing a first nucleotide sequence, wherein the first nucleotide sequence comprises a coding strand, the coding strand comprising one or more regulatory elements and a FOXP3 gene or portion thereof providing a nuclease and performing a gene editing process on thefirst nucleotide sequence, which edits said one or more regulatory elements, and optionally edits the FOXP3 gene or portion thereof. Methods of treating a subject suffering from an autoimmune diseaseand subjects suffering the effects of organ transplantation are also provided.

The invention provides a DNA molecule comprising a multicistronic transcription unit coding for i) a polypeptide of interest, and for ii) a selectable marker polypeptide functional in a eukaryotic host cell, wherein the polypeptide of interest has a translation initiation sequence separate from that of the selectable marker polypeptide, and wherein the coding sequence for the polypeptide of interest is upstream from the coding sequence for the selectable marker polypeptide in the multicistronic transcription unit, and wherein an internal ribosome entry site is present downstream from the coding sequence for the polypeptide of interest and upstream from the coding sequence for the selectable marker polypeptide, and wherein the nucleic acid sequence coding for the selectable marker polypeptide in the coding strand comprises a GTG or a TTG start codon. Also provided are methods for obtaining host cells expressing a polypeptide of interest, such host cells comprising DNA molecules of the invention. Further provided is the production of polypeptides of interest, comprising culturing host cells comprising the DNA molecules of the invention.

The present invention provides a new gene sequence and its application. The gene sequence includes the following characteristics: the first deoxynucleoside of the first codon of the coding chain of the giant embryo allele corresponding to the normal embryo rice "Nipponbare" An adenine deoxynucleotide is inserted between the upstream ‑777 and ‑778 deoxynucleotides of the acid, and at +125 downstream of the first deoxynucleotide of the first codon in the coding strand, a guanine Deoxynucleotides are mutated to adenine deoxynucleotides. The GABA content and vitamin E content of rice brown rice homozygous for this gene sequence are significantly higher than those of rice brown rice without this gene sequence. At the same time, the glutathione and maltooligosaccharide content in brown rice are also significantly higher than those without this gene sequence Rice Brown Rice Raised. Therefore, by transferring the gene sequence into normal embryo rice and obtaining homozygous giant embryo offspring, a new variety of giant embryo rice with a giant embryo phenotype and the content of various nutrients in brown rice can also be significantly improved.