The invention relates to a gene chip for evaluating a transport stress model and an application of the gene chip for evaluating the transport stress model. The gene chip comprises one or a plurality of probes used for detecting ribos nucleic acid (RNA), complementary RNA (cRNA) or complementary deoxyribose nucleic acid (cDNA) which is generated by the following genes: Gdnf, Lifr, Ppp3rl, Sphkl, B4galtl, Phldal, Il24, Adm, Gsdmal, Acvrl, Naip2, Aqp2, Ptprc, Bcl2l1, Rela, Ctnnb1, Pdcd6ip, Tgfbr1, Hmox1, Sort1, Tpt1, Aldh1a1, Actc1, Rhot2 and Bax. According to the chip, the typical variations of indexes after livestock and poultry are subjected to transport stress are comprehensively evaluated in a multilayer multi-target point manner through once real-time synchronous high throughput detection of the variation of nerve-immunity-internal secretion indexes, and guiding significance in livestock and poultry organism conditions and production performances during a transport evaluating process is obtained. The chip not only has the characteristics of sample saving and time saving in new drug research, but also saves money and has an important practical meaning.