42 results about "Template switching" patented technology

The present invention relates generally to methods for single-cell nucleic acid profiling, and nucleic acids useful in those methods. For example, it concerns using barcode sequences to track individual nucleic acids at single-cell resolution, utilizing template switching and sequencing reactions to generate the nucleic acid profiles. These methods and compositions are also applicable to other starting materials, such as cell and tissue lysates or extracted / purified RNA.

The present invention combines the use of template switching with nucleic acid amplification—such as PCR—to generate amplification products representative of an entire RNA population. An RNA polymerase promoter sequence allows transcription-based amplification to be performed on the derived amplification products such that antisense amplified RNA (aRNA) or complementary RNA (cRNA) is produced for subsequent downstream applications.

The invention provides a device for intelligently configuring an operation interface of electronic equipment, which is connected with a server through a transmission unit arranged in the device. The device comprises a central processing unit, an interface template unit and a template switching unit, wherein the interface template unit and the template switching unit are respectively connected with the central processing unit; the interface template unit is used for storing a plurality of operation interface templates scheduled by the central processing unit, and each operation interface template comprises a plurality of application programs; the template switching unit is used for sending a switching signal to the central processing unit so as to schedule a corresponding interface template; a clock unit is used for supplying a user use operation interface template and the time of a corresponding application program to the central processing unit; and the central processing unit is used for accumulatively recording use habits of the electronic equipment at different times and different places and use habits of the application program on the operation interface and intelligently regulating the operation interface of the electronic equipment according to the use habits to ensure that the operation interface of the electronic equipment better accords with the actual requirement on a user, therefore, the user experience is effectively enhanced.

A method, an apparatus and a system for a rapid motion search applied in template matching are disclosed. The method includes: selecting motion vectors of blocks related to a current block as candidate motion vectors of the current block; after the uniqueness of a series of the candidate motion vectors of the current block is maintained, calculating the cost function of the candidate motion vectors in a corresponding template area of a reference frame, and obtaining the motion vector of the best matching template from the candidate motion vectors of the current block. In the embodiments of the present invention, there is no need to determine a large search range and no need to determine the corresponding search path template, and it is only necessary to perform a search in a smaller range.

A method of preparing a DNA copy of a target polynucleotide using template switching is described. The method includes mixing a double stranded template / primer substrate made up of a DNA primer oligonucleotide associated with a complementary oligonucleotide template strand with a target polynucleotide in a reaction medium and adding a suitable amount of a non-retroviral reverse transcriptase to the reaction medium to extend the DNA primer oligonucleotide from its 3′ end to provide a DNA copy polynucleotide. The DNA copy polynucleotide includes a complementary target DNA polynucleotide that is synthesized using the target polynucleotide as a template. Methods of adding nucleotides to the double stranded template / primer substrate are also described. The method can be used to facilitate detection, PCR amplification, cloning, and determination of RNA and DNA sequences.

The invention relates to a safety control redundant system and method for a fully-intelligent master control system, belonging to the field of industrial control. The system and the method are used for solving the problems that the accuracy of a control result is not fully taken into account and the self-recovery can not be realized after failure of a control scheme adopting a distributed control system (DCS) of a dual machine temperature backup control mode. According to the system, a repeated two-out-of-three voting manner is adopted for the acquisition and processing of data information in the system, the accuracy of system input is guaranteed through carrying out two-out-of-three voting on acquired data, the accuracy of system calculation is guaranteed through carrying out two-out-of-three voting on an operation result on control templates, a control template with the best health condition is guaranteed to serve as a master control template through dynamic master control template switching, the accuracy of the system is further improved, and thus, the aim of safety control of the system is realized. The self-recovery of a failure condition of the control template is realized through the seamless reconstruction of the control template in failure, so that the continuity and stability of work of the control system are guaranteed.

Disclosed herein include systems, methods, compositions, and kits for 5′-based gene expression profiling. Some embodiments provide synthetic particles (e.g., beads) associated with a first plurality of oligonucleotide barcodes and a second plurality of oligonucleotide barcodes. In some embodiments, nucleic acid targets (e.g., mRNAs) are initially barcoded on the 3′ end with the first plurality of oligonucleotide barcodes and subsequently barcoded on the 5′ end following a template switching reaction and intermolecular hybridization with the first plurality of oligonucleotide barcodes and extension. Immune repertoire profiling methods are also provided in some embodiments.

The present invention provides a new approach to creating novel polynucleotide sequences by point mutation and recombination in vitro of a set of parental sequences. The new polynucleotide sequences can be useful in themselves or they can be used for the templated synthesis of polymers, e.g. polypeptides composed of α-amino acids as occurring naturally in protein synthesis or polymers comprised of β-amino acids or other building blocks as described (Templated molecules).

Disclosed herein include systems, methods, compositions, and kits for labeling nucleic acid targets in a sample. In some embodiments, nucleic acid targets (e.g., mRNAs) are initially barcoded on the 3′ end and are subsequently barcoded on the 5′ end following a template switching reaction and intermolecular and / or intramolecular hybridization and extension. 5′- and / or 3′-barcoded nucleic acid targets can serve as templates for amplification reactions and / or random priming and extension reactions to generate a sequencing library. The method can comprise generating a full-length sequence of the nucleic acid target by aligning a plurality of sequencing reads. Immune repertoire profiling methods are also provided in some embodiments.

The invention discloses a method for achieving user-defined graphical user interface. The method includes setting a template pack catalog in a graphical user interface material catalog; respectively storing templates in a folder manner according to template names in the template pack catalog, setting a configuration file of the user-defined graphical user interface in the graphical user interface material catalog, and setting template option values in the configuration file; adding an image file designating naming rules in a designation material catalog, wherein the image file is identified as an icon of the designation material catalog or file; reading the template option values in the configuration file, loading materials of each optional template, displaying preview images and template switching buttons of each optional template, and selecting corresponding templates according to operations of a user; reading the configuration file and catalog and file listings in the graphical user interface material catalog, identifying configuration items and the naming rules, correspondingly processing each rule and configuration, and displaying the graphical user interface according to processing results. The invention further discloses a device for achieving the user-defined graphical user interface.

This application discloses methods for cDN′A synthesis with improved reverse transcription, template switching and preamplification to increase both yield and average length of cDNA libraries generated from individual cells. The new methods include exchanging a single nucleoside residue for a locked nucleic acid (INA) at the TSO 3′ end, using a methyl group donor, and / or a MgCb concentration higher than conventionally used. Single-cell transcriptome analyses incorporating these differences have full-length coverage, improved sensitivity and accuracy, have less bias and are more amendable to cost-effective automation. The invention also provides cDNA molecules comprising a locked nucleic acid at the 3′-end, compositions and cDNA libraries comprising these cDNA molecules, and methods for single-cell transcriptome profiling.

The invention discloses a method and a device for template rendering. The method includes the following steps: acquiring a corresponding template and generating a corresponding Java-type file according to a web page browsing request of a user, and calling a confirmed variable type and / or a confirmed variable operation method in the template by using a Java direct calling method; and then, executing the Java-type file to generate a corresponding web page, and returning the wed page to the user. Thus, code execution efficiency in template switching can be effectively improved, the number of query per second of a service platform can be improved, response time is shortened, and therefore garbage recovery of a Java virtual machine of a system can be reduced, then performance of a whole application system can be improved, and performance bottlenecks caused by the template rendering can be avoided.

The invention relates to a template switching method for construction of an integrated process of product reliability, maintainability and supportability (RMS) and performance. The template switching method comprises the following steps: (1) selecting an integrated process template according to the product, and by adding, deleting and modifying, constructing an initial integrated process to be optimized; (2) by using the bilateral switching relation between a process directed graph and a design structure matrix, constructing an initial design structure matrix N on the basis of determining the information dependency relation among working items; (3) solving an adjacent matrix A and a reachable matrix R corresponding to N, and a product of R and a transposed matrix RT thereof to find out a coupled set in the matrix; (4) sequencing the working items in the coupled set by a segmentation algorithm, an aggregation algorithm and a tearing algorithm; (5) regarding the coupled set as a working item to be sequenced and graded with other independent items, and forming the design structure matrix after optimization; (6) by using the bilateral switching relation between the process directed graph and the design structure matrix, switching the design structure matrix after optimization to be the integrated design process of RMS and performance to be determined; and (7) inspecting the integrated process of RMS and performance to be determined by a standard inspection list, and forming a new integrated process template for later use.

The invention discloses a template engine realization method used for Web development. The template engine realization method comprises the following specific implementation processes: designing multiple sets of general html templates for switching template styles; corresponding to different business pages to develop and design a HTML(Hypertext Markup Language) / XML (Extensive Markup Language) generator; operating business logics and data in advance, displaying a final result by the generated HTML, and dynamically displaying business data by the tag of the HTML; adding a style switching entry for background management to achieve a template switching effect; and revising contents in the template by interface design personnel, and revising the generator by program development personnel to finish the maintenance of a whole module. Compared with the prior art, the template engine realization method used for Web development has a simple integral realization process, C# codes are separated from a web page, so that convenience is brought for the long-term maintenance of a web system, and the template engine realization method has the advantages of being high in practicality, wide in applicable range and easy in popularization.

The invention relates to the field of wave-soldering and particularly provides an auxiliary soldering device and method for PCB components. The auxiliary soldering device comprises carrier shielding templates and a template switching mechanism. The carrier shielding templates comprise the left template and the right template, first through holes used for exposing first soldering positions of a PCB are formed in the left template, second through holes used for exposing second soldering positions of the PCB are formed in the right template, and the template switching mechanism is used for controlling the left template and the right template to be switched at the position of a wave crest spraying opening. When soldering at the first soldering positions is finished, soldering at the second positions is directly conducted after the right template is switched to the position of the wave crest spraying opening, and separated soldering of the first soldering positions and the second soldering positions is achieved; furthermore, due to the fact that only one carrier and one wave crest spraying opening are needed, the production and maintenance cost is lowered, and the production technology is simplified.

Disclosed herein, are compositions, methods, and kits comprising engineered reverse transcription enzymes that exhibit several desired properties such as thermal stability, processive reverse transcription, non-templated base addition, and template switching ability. The engineered reverse transcription enzymes described herein demonstrate unexpectedly higher resistance to cell lysate inhibition,greater ability to capture full-length mRNA transcripts, and demonstrate improved results in small reaction volumes as compared to other engineered reverse transcription enzymes.

A method of preparing a DNA copy of a target polynucleotide using template switching is described. The method includes mixing a double stranded template / primer substrate made up of a DNA primer oligonucleotide associated with a complementary oligonucleotide template strand with a target polynucleotide in a reaction medium and adding a suitable amount of a non-retroviral reverse transcriptase to the reaction medium to extend the DNA primer oligonucleotide from its 3′ end to provide a DNA copy polynucleotide. The DNA copy polynucleotide includes a complementary target DNA polynucleotide that is synthesized using the target polynucleotide as a template. Methods of adding nucleotides to the double stranded template / primer substrate are also described. The method can be used to facilitate detection, PCR amplification, cloning, and determination of RNA and DNA sequences.

The invention discloses a high-throughput sequencing library construction method for trace free RNA of body fluid. The method comprises the following steps: purifying a body fluid sample exRNA with asilicon substrate, performing dephosphorylation, adding polyA tails, and performing inverse transcription on tailing products by using template switching and nucleic acid locking technologies so as toobtain cDNA of which the 5'-end is provided with linkers; and performing low-cycle PCR (Polymerase Chain Reaction), and finally obtaining the high-throughput sequencing library. According to the method disclosed by the invention, by using the template switching and nucleic acid locking technologies, deep optimization is performed on problems encountered by trace RNA in the process of constructingthe high-throughput sequencing library, and the library construction time and cost can be greatly reduced. By using the technology in the invention, library construction can be completed on less than10ng of trace RNA in one day, high-quality data can be acquired by virtue of high-throughput sequencing, and many existing disease-related RNA biomarkers can be found by analysis.

The invention discloses an online coiled material appearance inspection machine. The online coiled material appearance inspection machine is composed of an upper rack, a lower rack, a discharging mechanism, an encoder, a light source, an inspection platform, an operation platform, a power mechanism, an inspection system, a defective product treatment platform, a laminating mechanism, a material collecting mechanism, a display system and a guiding mechanism. The discharging mechanism and the material collecting mechanism are arranged at the two ends of the upper rack correspondingly. The inspection platform is arranged on the lower rack through a support. The operation platform is arranged at the top end of the lower rack. The power mechanism and the guiding mechanism are symmetrically arranged on the two sides of the part, at the top end of the lower rack, of the support. The inspection system is arranged at the top end of the support. The defective product treatment platform is arranged at the top end of the part, between the support and the material collecting mechanism, of the lower rack. The laminating mechanism is located at the top end of the part, between the inspection system and the material collecting mechanism, of the upper rack. The online coiled material appearance inspection machine is high in inspection efficiency, online inspection efficiency is higher than manual visual inspection efficiency by five or more times, the misjudgment rate is low, many items can be inspected, template switching is rapid, and the strict degree for product inspection conditions can be adjusted according to actual requirements.

A method, an apparatus and a system for a rapid motion search applied in template matching are disclosed. The method includes: selecting motion vectors of blocks related to a current block as candidate motion vectors of the current block; after the uniqueness of a series of the candidate motion vectors of the current block is maintained, calculating the cost function of the candidate motion vectors in a corresponding template area of a reference frame, and obtaining the motion vector of the best matching template from the candidate motion vectors of the current block. In the embodiments of the present invention, there is no need to determine a large search range and no need to determine the corresponding search path template, and it is only necessary to perform a search in a smaller range.

The invention provides a business flow chart template switching method and device. The method comprises the steps of acquiring relation information between nodes in a business flow chart under a first template, wherein the relation information includes leader-member relations between the nodes and connecting line states of each node and the other nodes; acquiring a first design parameter of each node in the business flow chart under the first template; acquiring a second node size of the business flow chart under a second temperate selected by a user; according to the relation information, the first design parameter and the second node size, generating a second design parameter of each node in the business flow chart under the second template; forming a target business flow chart under the second template according to the second design parameter. The method enables the same business flow chart to be switched freely among multiple templates and solves the problem that new business flow charts need to be developed according to requirements of users and thus time costs are high in the prior art.

The invention discloses a vehicle body large part positioning tool with a template switching function, and belongs to the field of auxiliary positioning tools of railway vehicle body large parts. The positioning tool comprises double guide rails and a template positioning base, wherein the double guide rails consist of two identical guide rails; chutes with outer side stops are formed in the upper end surfaces of the guide rails; the two identical guide rails are symmetrically arranged in parallel; the template positioning base comprises two square baffle plates and a clamping plate component; the clamping plate component comprises four clamping plates with through grooves, and multiple template positioning screw holes; and the four clamping plates are integrally molded, and are arranged at intervals by circumference angles. Vehicle body large part templates can quickly finish the switching of selected template stations according to actual requirements, so that the operation of replacing the vehicle body large part templates in batches is convenient and quick, the production efficiency can be largely improved, the production cost is saved, and the benefit is created. In addition, the positioning tool has such advantages as simple and practical structure, convenience for operation, low cost and convenience for promotion and popularization.

This application discloses methods for cDN′A synthesis with improved reverse transcription, template switching and preamplification to increase both yield and average length of cDNA libraries generated from individual cells. The new methods include exchanging a single nucleoside residue for a locked nucleic acid (INA) at the TSO 3′ end, using a methyl group donor, and / or a MgCb concentration higher than conventionally used. Single-cell transcriptome analyses incorporating these differences have full-length coverage, improved sensitivity and accuracy, have less bias and are more amendable to cost-effective automation. The invention also provides cDNA molecules comprising a locked nucleic acid at the 3′-end, compositions and cDNA libraries comprising these cDNA molecules, and methods for single-cell transcriptome profiling.

Presented herein are methods and compositions for multiplexed single cell gene expression analysis. Some methods and compositions include the use of droplets and / or beads bearing unique barcodes such as unique molecular barcodes (UMI).

High throughput methods and compositions (e.g., kits) for the amplification of RNA fragments, including in particular, for the detection of fusion mutations in a high volume of samples, e.g., by high throughput sequencing method. These methods may include barcoding cDNA preparations with template switching reactions, indexing pools of libraries and intensive use of automatic liquid handling, and providing a ready-to-sequence library mix.

The invention discloses a method and a device for template rendering. The method includes the following steps: acquiring a corresponding template and generating a corresponding Java-type file according to a web page browsing request of a user, and calling a confirmed variable type and / or a confirmed variable operation method in the template by using a Java direct calling method; and then, executing the Java-type file to generate a corresponding web page, and returning the wed page to the user. Thus, code execution efficiency in template switching can be effectively improved, the number of query per second of a service platform can be improved, response time is shortened, and therefore garbage recovery of a Java virtual machine of a system can be reduced, then performance of a whole application system can be improved, and performance bottlenecks caused by the template rendering can be avoided.

The invention relates to a safety control redundant system and method for a fully-intelligent master control system, belonging to the field of industrial control. The system and the method are used for solving the problems that the accuracy of a control result is not fully taken into account and the self-recovery can not be realized after failure of a control scheme adopting a distributed control system (DCS) of a dual machine temperature backup control mode. According to the system, a repeated two-out-of-three voting manner is adopted for the acquisition and processing of data information in the system, the accuracy of system input is guaranteed through carrying out two-out-of-three voting on acquired data, the accuracy of system calculation is guaranteed through carrying out two-out-of-three voting on an operation result on control templates, a control template with the best health condition is guaranteed to serve as a master control template through dynamic master control template switching, the accuracy of the system is further improved, and thus, the aim of safety control of the system is realized. The self-recovery of a failure condition of the control template is realized through the seamless reconstruction of the control template in failure, so that the continuity and stability of work of the control system are guaranteed.