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Tissue culture method of ginseng adventitious root

The invention discloses a tissue culture method of ginseng adventitious root, comprising the steps of induction, amplification and successive transfer culture of ginseng callus; the induced callus is inoculated on the prepared solid culture medium and added with plant growth regulator to be cultured for 3-4 weeks under the conditions of sterility, the temperature of 23-25 DEG C and keeping in dark place; the amplified callus is inoculated on the prepared solid culture medium and added with proper plant growth regulator to be cultured for 3-5 weeks under the conditions of sterility, the temperature of 23-25 DEG C and keeping in dark place, and then the adventitious root can be formed; the formed adventitious root is picked out and inoculated on the prepared liquid culture medium and added with the plant growth regulator to be cultured for 4-5 weeks under the conditions of sterility, room temperature and sunshine. The method has higher actual significance than ginseng cell cultivation, and the growth speed of the adventitious root is much higher than that of the original root; furthermore, the production rate of active secondary metabolite of the adventitious root is high and stabler and is not influenced by natural environment such as natural disaster and the like; the tissue culture method also has the characteristics of simple technique process, high inductivity, good repeatability, rapid propagation of the adventitious root, etc.
Owner:TIANJIN UNIV
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