A
biosensor in the form of a strip. In one embodiment, the
biosensor strip comprises an
electrode support, a first
electrode, i.e., a
working electrode, a second
electrode, i.e., a counter electrode, and a third electrode, i.e., a
reference electrode. Each of the electrodes is disposed on and supported by the electrode support. Each of the electrodes is spaced apart from the other two electrodes. The
biosensor strip can include a covering layer, which defines an enclosed space over the electrodes. This enclosed space includes a zone where an
analyte in the sample reacts with
reagent(s) deposited at the
working electrode. This zone is referred to as the
reaction zone. The covering layer has an aperture for receiving a sample for introduction into the
reaction zone. The biosensor strip can also include at least one layer of mesh interposed in the enclosed space between the covering layer and the electrodes in the
reaction zone. This layer of mesh facilitates transporting of the sample to the electrodes in the reaction zone. In another embodiment, a biosensor strip can be constructed to provide a configuration that will allow the sample to be introduced to the reaction zone by action of capillary force. In this embodiment, the layer of mesh can be omitted. The invention also provides a method for determining the concentration of glucose in a sample of
whole blood by using the biosensor of this invention.