10 results about "Local Reaction" patented technology

The invention relates to the technical field of space-time transcriptomes, in particular to a space-time transcription technology-based heterologous target sequence specific enrichment method and application. The invention provides a heterologous target sequence specific enrichment method based on a space-time transcription technology and application. In the reaction process of space-time transcriptome analysis, the method provided by the invention can be used for specifically enriching interested specific sequences (heterologous target sequences) while space-time gene information of the whole tissue slice is reserved, so that the relationship between heterologous genes and gene expression modes in tissues can be better researched. According to the method provided by the invention, an enrichment process based on combination of space transcriptome and multi-omics analysis is perfected, and a reliable basis is provided for multi-omics research, for example, bacterial distribution information in a host, infection mode information of the host, local response of the host and the like can be obtained from a bacterial infected tissue sample.

The invention develops a self-driving and self-catalyzing method for detecting microRNA-21 (miR-21) on the basis of a three-legged DNA (deoxyribonucleic acid) nano machine. If a sample to be detected contains miR-21, the miR-21 can open an arm 1 of the three-legged DNA nano machine through a proximity effect, and the arm 1 and a nearby arm 2 form a DNA enzyme structure. The DNA enzyme can cut a substrate chain on the arm 2, the DNA arm 2 exposed after cutting is dissociated to compete with the arm 3, and a DNA fragment (sequence Q) marked with a quenching group is generated to leave the DNA nano-machine, so that a fluorescence signal is recovered. Meanwhile, the released sequence Q can catalyze the next self-driven and self-catalyzed circular reaction of the three-legged DNA nano machine. The component with close space positioning is constructed on a single DNA nanostructure, so that the local reactant concentration and the reaction rate are remarkably improved. And the fluorescence signal and the miR-21 have a linear relationship between the concentration of 10 pM and 50 nM. Compared with a free diffusion reactant, the effective concentration of the reactant is greatly increased. Therefore, the incubation time is obviously shortened to 35 minutes. The strategy has important significance in the aspect of providing reference value for miRNA detection and disease diagnosis.

A kit (100) and method (200) are provided for administering an active ingredient (112) to a subject to elicit a response. The kit comprises a first container (110) with an active ingredient (112) and a second container (120) with an adjuvant (122). The containers permit the ingredients to be admixed to form a compounded preparation for delivery into the subject's tissue. The active ingredient can comprise an allergen. The kit can also include temperature control and mixing components, potentially involving a third container (130). The method comprises providing the kit (100), admixing the ingredients, and injecting the compounded form into the subject's tissue such as a lymph node. The injection can be monitored and repeated. The approach can elicit various responses such as desensitization according to the type of active ingredient used. Overall, the kit (100) and method (200) provide targeted delivery of active ingredients to efficiently provoke a localized response in the subject.

A formulation of naltrexone that ameliorates undesirable localized reactions at the site of implantation.

The invention discloses a breast cancer cell precise imaging platform based on a self-energized DNAzyme logic circuit and application of the breast cancer cell precise imaging platform, a DNAzyme element capable of realizing dual-microRNA logic detection after sequence modification and a substrate hairpin element of the DNAzyme element construct an intracellular logic circuit for breast cancer cell recognition, and in order to enhance the output capability of the circuit and the stability of intracellular operation, the DNAzyme element can realize dual-microRNA logic detection. The DNA is integrated on a DNA tetrahedron, and the problems of output capacity and stability are solved by utilizing local reaction concentration increase caused by a space confinement effect of the tetrahedron and an anti-enzymolysis effect enhanced by high nucleic acid concentration; meanwhile, by means of the characteristic that MnO2 nanosheets are degraded into Mn < 2 + > by GSH when exposed to a tumor microenvironment, self-sufficiency of in-situ divalent metal ions is achieved, an intelligent imaging platform is constructed and applied to accurate imaging of breast cancer cells, and a new thought and technical support are provided for early diagnosis and prognosis improvement of breast cancer.

The present invention proposes the use of a tool (13) that is connected to a device for locomotion, such as, for example, a robot, and provided with an umbilical cable (06) to bring about a controlled reaction near the blockage, in order to remove it. In order to carry out that controlled reaction, an injection, and control system is used, which may be a closed loop control system.

The invention relates to the technical field of medical auxiliary instruments, in particular to a uterine cavity full-suture anchoring device which is characterized in that the uterine cavity full-suture anchoring device comprises an outer sheath, an inner sheath, a puncture needle, a fixing line and a full-suture anchor nail, a first cavity penetrating through the two ends of the outer sheath is formed in the outer sheath, the inner sheath penetrates through the first cavity, a second cavity penetrating through the two ends of the inner sheath is formed in the inner sheath, and the puncture needle is arranged in the second cavity. The puncture needle is arranged in the second cavity in a penetrating mode, the full-suture anchor is arranged in the middle of the fixing line in a sleeved mode, the fixing line is bent from the middle of the full-suture anchor to be in a U shape and forms two line tails, and a needle head of the puncture needle detachably hooks the middle of the full-suture anchor and enables the two line tails of the fixing line to be arranged in the second cavity in a penetrating mode. Under the cooperation of the full-suture anchor, the middle of the fixing line can be fixed in the uterine muscle layer, the fixing line is not prone to falling off, the bearing capacity is high, fixing is firm, local reaction is small, operation is easy and convenient, and the effect of fixing the contraceptive ring or the medicine ring is achieved by knotting and fixing the fixing line and the tail wire of the contraceptive ring or the medicine ring.

This invention provides a programmable mechanical metamaterial structure generation method and system based on a reaction-diffusion morphogenesis mechanism. By establishing a reaction-diffusion kinetic model, the structural morphology self-organizes under the coupling effect of local reaction and spatial diffusion, thereby generating structural morphologies with complex topological features and aperiodic spatial distribution characteristics. By setting spatial parameter fields such as diffusion coefficient, supply rate, and disappearance rate within the design domain, and introducing a direction control field to adjust the diffusion direction, the scale characteristics, connectivity, and anisotropy characteristics of the generated structure can be regionalized and continuously controlled. Through threshold transformation, boundary extraction, and geometric reconstruction of the morphology generation results, the generated structure can be directly converted into a two-dimensional or three-dimensional geometric model. This invention expands the design space of mechanical metamaterial structures and realizes an effective connection between structure generation and engineering design and manufacturing.

The utility model relates to medical instrument technical field especially, and more particularly to a kind of measuring scale of vaccine clinical test inoculation local reaction.The technical scheme, including measuring scale main part, is provided with graduated scale for vaccine inoculation site reaction on the measuring scale main part;The measuring scale main part includes the first circular disc, second circular disc, third circular disc and fourth circular disc of increasing diameter in turn;The first circular disc, second circular disc, third circular disc and fourth circular disc and connecting strip are all transparent flexible material.The utility model can quickly and accurately measure the maximum diameter of irregular shape, reduce measurement error, can be visually fast and carry out the grading evaluation of local reaction, use disposable film, reduce the risk of skin infection.

A formulation of naltrexone that ameliorates undesirable localized reactions at the site of implantation.