4091 results about "Active site" patented technology

A method for achieving site specific integration of a desired DNA at a target site in a mammalian cell via homologous recombination is described. This method provides for the reproducible selection of cell lines wherein a desired DNA is integrated at a predetermined transcriptionally active site previously marked with a marker plasmid. The method is particularly suitable for the production of mammalian cell lines which secrete mammalian proteins at high levels, in particular immunoglobulins. Novel vectors and vector combinations for use in the subject cloning method are also provided.

Convenient techniques for discriminating the base type in a base sequence of a nucleic acid are provided. The technique includes the step (a) of preparing a sample solution containing a nucleic acid, a primer having a base sequence that includes a complementary binding region which complementarily binds to the nucleic acid, and a nucleotide; the step (b) of allowing the sample solution to stand under a condition to cause an extension reaction of the primer, and producing pyrophosphate when the extension reaction is caused; the step (c) of bringing the sample solution into contact with the front face of a H+ hardly permeable membrane having H+-pyrophosphatase, which penetrates from front to back of the membrane, of which active site that hydrolyzes pyrophosphate being exposed to the front face; the step (d) of measuring the H+ concentration of at least either one of the solution at the front face side of the H+ hardly permeable membrane or the solution at the back face side of the H+ hardly permeable membrane, in a state where the H+-pyrophosphatase is immersed in the solution; the step (e) of detecting the extension reaction on the basis of the result of measurement in the step (d) ; and the step (f) of discriminating the base type in the base sequence of the nucleic acid on the basis of the result of detection in the step (e).

New RNA cap analogs are disclosed containing one or more phosphorothioates groups. The analogs also contain modifications at the 2′-O position of 7-methylguanosine that prevent them from being incorporated in the reverse orientation during in vitro synthesis of mRNA and that hence are “anti-reverse cap analogs” (ARCAs). The ARCA modification ensures that the S atom is precisely positioned within the active sites of cap-binding proteins in both the translational and decapping machinery. The new S-ARCA analogs are resistant to in vivo decapping enzymes. Some S-ARCAs have a higher affinity for eIF4E than the corresponding analogs not containing a phosphorothioate group. When mRNAs containing the various S-ARCAs are introduced into cultured cells, some are translated as much as five-fold more efficiently than mRNAs synthesized with the conventional analog m7GpppG.

A method for reducing odor is provided. The method comprises forming a coordination complex between a transition metal and a polydentate compound, and contacting the coordinated complex with an odorous compound. The transition metal provides one or more active sites for capturing the odorous compound. In some embodiments, the polydentate compound may be a polyalkylimine, such as polyethyleneimine, polypropyleneimine, or a dendrimer thereof.

The invention discloses a bulk hydrotreating catalyst and a preparation method thereof. The catalyst comprises three active metal components, namely Mo, W and Ni, and the sum of active metal oxides is 30 to 70 weight percent. In the method, a proper amount of water-soluble nitrogen-containing compound is added in the bulk catalyst gelatinating process, the pore structure of the catalyst is improved, more metal active sites are exposed on the surface of the catalyst, the utilization rate of active metals is improved, the catalyst has obviously high activity compared with the common bulk catalyst under the condition of the same metal content, and the consumption of the active metals for the common bulk catalyst can be reduced and the preparation cost of the catalyst can be reduced under thecondition of the equivalent activity. In addition, the bulk catalyst has increased pore diameter and pore volume, the Ni-W high active center is fully utilized, complicated macromolecules are easier to contact the active center, and the effect is particularly more obvious when the catalyst treats distillates with high macromolecule content.

The present invention is concerned with new compounds, and particularly those having a fused bicyclic ring substituted with an amidine moiety. These compounds are each potent inhibitors of Factor D of the alternate pathway of complement, C1s of the classical pathway of complement, Factors Xa, XIIa, VIIa and thrombin of the coagulation pathway, plasmin in the fibrinolytic pathway, and kallikrein and high molecular weight kininogen in the inflammatory pathways. These proteases, which have serine in their active site, are called serine proteases and they are pivotal to most of the processes of inflammation and coagulation. In fact, these various systems are interactive with one another and it is difficult to activate one pathway without it influencing the others.

The invention discloses a high-activity catalyst which catalyzing and activating carbon dioxide to perform the copolymerization reaction with alkylene oxide to synthesize polycarbonate. The catalyst is characterized in that the catalyst is a tetradentate schiff base complex contains one or two organic base groups and has single active site initiation and controllable polymerization. The catalyst has the advantages that under milder conditions and at lower concentration of the catalyst, the catalyst can highly and efficiently catalyze the carbon dioxide to make the copolymerization reaction with the alkylene oxide to prepare the polycarbonate, the catalytic efficiency is 10<6>g polymer / mol catalyst, the polymer molecular weight is adjusted in the range of between 5,000 and 500,000, the molecular weight distribution is less than 2, the alternating structure is over 97 percent, and the catalyst can be degraded into small molecule compounds under certain condition. By utilizing the catalyst, the telomerization reaction between the carbon dioxide and two or more than two types of alkylene oxide can be catalyzed to synthesize a polycarbonate telomer with adjusting glass transition temperature.

New compositions and methods of using those compositions as bonding compositions are provided. The compositions comprise a polymer dispersed or dissolved in a solvent system, and can be used to bond an active wafer to a carrier wafer or substrate to assist in protecting the active wafer and its active sites during subsequent processing and handling. The compositions form bonding layers that are chemically and thermally resistant, but that can also be softened to allow the wafers to slide apart at the appropriate stage in the fabrication process.

The invention discloses a preparation method of a hydrogenation catalyst composition. According to the method, sodium meta-aluminate is adopted as an aluminum source in the preparation of a mixture of an NixWyOz composite oxide precursor and an Al2O3 precursor through a coprecipitation process, and a proper amount of a CO2 gas is accessed in the colloid forming process, so a difficult shaping problem of a bulk phase catalyst is solved, physicochemical properties of the catalyst are adjusted, and the composition which has the characteristics of large specific surface area and uniform aperture distribution and makes many metal active sites be exposed on the catalyst surface allows the utilization rate of active metals to be improved. The catalyst composition of the invention, which has increased apertures and pore volumes, allows the Ni-W high activity sites to be fully utilized and complex macrostructure molecules to easily contact with the active sites, so the catalyst composition is especially suitable for ultra-deep desulfurization reactions for the production of ultraclean diesel oil.

A build-up structure for chip to chip interconnects and System-In-Package utilizing multi-angle vias for electrical and optical routing or bussing of electronic information and controlled CTE dielectrics including mesocomposites to achieve optimum electrical and optical performance of monolithic structures. Die, multiple die, Microelectromechanical Machines (MEMs) and / or other active or passive components such as transducers or capacitors can be accurately positioned on a substrate such as a copper heatsink and multi-angle stud bumps can be placed on the active sites of the components. A first dielectric layer is preferably placed on the components, thereby embedding the components in the structure. Through various processes of photolithography, laser machining, soft lithography or anisotropic conductive film bonding, escape routing and circuitry is formed on the first metal layer. Additional dielectric layers and metal circuitry are formed utilizing multi-angle vias to form escape routing from tight pitch bond pads on the die to other active and passive components. Multi-angle vias can carry electrical or optical information in the form of digital or analog electromagnetic current, or in the form of visible or non-visible optical bussing and interconnections.

Compounds having the active site of natural lipoxins, but a longer tissue half-life are disclosed. In particular, 15-epi-lipoxins and their use in ameliorating undesired cell proliferation, which characterizes diseases such as cancer, are also disclosed.

A process and apparatus for the decontamination of gaseous contaminants (especially oxygen, carbon dioxide and water vapor) from hydride gases (including their lower alkyl analogs) down to <=100 ppb contaminant concentration are described. The critical component is a high surface area metal oxide substrate with reduced metal active sites, which in various physical forms is capable of decontaminating such gases to <=100 ppb, <=50 ppb or <=10 ppb level without being detrimentally affected by the hydride gases. The surface area of the substrate will be >=100 m2 / g, and preferably 200-800 m2 / g. Oxides of various metals, especially manganese or molybdenum, can be used, and mixtures of integrated oxides, or one type of oxide coated on another, may be used. The substrate is preferably retained in a hydride-gas-resistant container which is installed in a gas supply line, such as to a gas- or vapor-deposition manufacturing unit. The invention provides final decontamination for hydride gas streams intended for gas- or vapor-deposition formation of high purity LED, laser (especially blue laser), electronic, optical or similar products, and can be used in combination with upstream preliminary decontamination process and / or upstream or downstream solid particulate removal units.

A novel process for the selective elimination of fatty acid compounds containing carbon-carbon double bonds in trans configuration from a substrate containing cis- and trans-isomers of said fatty acid compounds, by selective adsorption by a microporous zeolite material is disclosed. The pore size and shape of usable zeolite materials enable differentiation between cis- and trans-isomers of unsaturated fatty acid chains. The zeolite materials used have a selectivity ratio alphatrans / cis higher than 1.00; this ratio is defined based on the elution properties of cis and trans double bond containing fatty acid methylesters dissolved in n-hexane during a column chromatography experiment with the zeolite material as the stationary phase and n-hexane as the mobile phase. Besides selective adsorption of trans-unsaturated fatty acid compounds, simultaneous or subsequent total or partial hydrogenation of the double bonds in said compounds can be carried out while using the same or similar zeolite material, containing finely dispersed catalytic active metals. The majority of these catalytic active sites must be inside the pores.

The invention provides a preparation method of a high-density transition metal monoatomic load graphene-based catalyst. The preparation method comprises the following steps of dissolving a nitrogen-containing precursor, a carbon source and a soluble transition metal salt, and performing evaporation drying, grinding and calcination to obtain the high-density transition metal monoatomic load graphene-based catalyst. According to the preparation method, only the soluble metal salt is utilized, glucose and the like are used as the carbon source, dicyandiamide is used as the nitrogen-doped source, and the high-efficiency catalyst with high active site density and high monoatomic loading quantity is successfully prepared only by one-step calcination method.

This invention relates to modified high surface area materials, such as nanoparticles that are coated with metal ions, and articles treated with the modified high surface area materials. The modified nanoparticles have active sites that bind various gases and / or odorous compounds, thereby removing these compounds from a medium such as air or water. Metal ions are adsorbed onto the surface of the nanoparticle and bound strongly to the surface. By selection of the metal ion, specific gaseous compounds and / or odorous compounds can be targeted and removed efficiently and effectively from both aqueous phase and from the air. The modified nanoparticles are applied to nonwoven webs to provide odor removing articles for industrial and consumer use in refrigerators and trash containers.

The invention discloses a preparation method of a ferro-nickel bi-metal hydroxide of a sheet structure on foamed nickel through in-situ growth, and belongs to the field of electrochemistry. Nickel nitrate hexahydrate, iron nitrate nonahydrate, urea, ammonium fluoride and foamed nickel are adopted as main raw materials, and an electrocatalyst through water electrolysis oxygen production is successfully prepared. The sheet structure of the catalyst supplies abundant active sites, due to existence of substrate foamed nickel, the electrical conductivity is enhanced, and when the hydroxide is adopted as the electro-catalysis oxygen production catalyst, the excellent catalysis activity is shown. The activity of a traditional non-noble metal material under an oxygen evolution reaction (OER) in analkaline electrolyte is not ideal. Accordingly, the OER catalysis performance of the non-noble metal material under the alkaline condition is improved, and the method is of great significance in solving the fossil fuel energy crisis. Nickel and iron earth reserve volumes are quite abundant, the OER catalytic performance of the ferro-nickel bi-metal hydroxide of the sheet structure under the alkaline condition is excellent, and the hydroxide is a catalytic material with the wide prospect.

The present invention is based, at least in part, on the identification of a novel active site on BCL-2 family polypeptide such as BAX, which when bound by a compound, modifies the activity of the BCL-2 family polypeptide.

The present invention relates to a process for producing a modified polymer, comprising modifying a polymer having an active site of an organometal type in a molecule by reacting the site thereof with a hydrocarbyloxysilane compound and adding a condensation accelerator to the reaction system in the middle of the above reaction and / or after completion thereof and a rubber composition comprising the modified polymer obtained by the process described above, preferably a rubber composition comprising 100 parts by weight of (A) a rubber component containing at least 3% by weight of the above modified polymer and 10 to 100 parts by weight of (B) silica and / or carbon black. According to the present invention, capable of being provided is a silica and / or carbon black-blended rubber composition which enhances interaction with silica and carbon black and which elevates the fracture characteristic, the abrasion resistance and the low heat buildup property and can exhibit a good workability.

The present invention relates to nucleic acid sequencing methods, kits and reagents, and more particularly to methods of sequencing nucleic acid which employ a nucleic acid processing enzyme and one or more nucleotide analogues that are capable of binding to the active site of the enzyme and to complementary bases in the nucleic acid molecule being sequenced, but which are non-incorporable or inhibitors of the nucleic acid processing enzyme. In further aspects, the present invention relates to conjugates which comprise a deoxyribonucleotide triphosphates (DNTPs) or an analogue thereof linked to an intercalating dye.

To provide a method for producing a wide variety of carbazole derivatives which have a simple and uncomplicated process and in which variations in the yield, purity, etc. of a desired substance which are caused by an aryl group introduced is reduced as much as possible. A method for producing a carbazole derivative represented by General Formula (1) is provided, in which 9-[4-(10-phenyl-9-anthryl)phenyl]-9H-carbazole having an active site at the 3-position of the carbazole skeleton and an aromatic compound having an active site are coupled.In the formula, Ar1 represents an aryl group with 6 to 13 carbon atoms in a ring, and Ar1 may have a substituent.

Embodiments of the present invention relate to a system and method for controlling a tissue treatment device using results from a spectroscopic tissue analyzer. One embodiment provides a system that uses a direct signal from a spectroscopic tissue analyzer to control the tissue treatment device. Alternatively, the system may provide an audible feedback signal to a user, allowing the user to choose how to adjust the tissue treatment device. A final embodiment provides a probe that has active sites for treating tissue, and an optical system to convey light to the active sites, allowing an analysis of the tissue parameter at the active sites.

The present invention discloses a heteratom doped carbon nano-tube-graphene complex and a preparation method thereof, and belongs to the technical field of new material preparation. The complex comprises carbon nano-tubes and graphene, wherein heteroatoms are introduced into the complex. The preparation method comprises: adopting catalyst active component loading layered material as a catalyst, adopting chemical vapor deposition, and growing the heteratom doped carbon nano-tube and graphene complex in the presence of a heteratom source. The heteratom doped carbon nano-tube-graphene complex has excellent characteristics of more active sites, good conductivity, easy chemical modification and the like based on maintaining of intrinsic e excellent performances of carbon nano-tubes and graphene, and provides good application prospects in the fields of catalysis, electrochemistry, energy storage and conversion, electronic devices, and the like. The preparation method has characteristics of simpleness, easy engineering magnification, and macroscopic quantity preparation achievement so as to promote carbon nano-tube-graphene complex industrial application.

The present invention provides a bimodal porous carbon capsule with a hollow core and a mesoporous shell structure, which can be employed as an electrocatalyst support for a fuel cell; electrocatalysts for the fuel cell using the bimodal porous carbon capsule, and a method of preparing the same. The electrocatalyst according to the present invention has higher catalysis activity as compared with the Pt—Ru or Pt catalyst supported by the conventional carbon black, so that the performance of the fuel cell is enhanced, and it can be easily prepared in an aqueous solution state. According to the present invention, the porous carbon support employed as the support for the catalyst has excellent conductivity and a high surface area, so that the loaded catalyst can be prepared with a smaller amount than that of the conventional carbon black. Further, metal particles having an extremely fine size of 2˜3 nm are uniformly distributed on the support, so that the area of an active site at which catalysis reaction is performed is increased, thereby increasing the catalyst activity with respect to the oxidation reaction of the fuel such as methanol, ethanol, hydrogen, etc. Also, a fine pore of a porous carbon support secures a fuel dispersing passage, so that the fuel including alcohol such as methanol, ethanol or the like, hydrogen, etc. can be easily transferred and dispersed, thereby efficiently performing its oxidation-reduction reaction. On the other hand, an air electrode can efficiently function as the catalyst due to the same principle.

The compound (W-Z-M) according to the invention comprises a component (M) chosen from the group consisting of markers and therapeutic agents possessing an intracellular active site (A.S.), linked to a ligand (W-Z) comprising an arm (Z) linked to a terminal group (W), characterized in that the linkage between the arm (Z) of the ligand (W-Z) and the component (M) prevents intracellular entry of the compound (W-Z-M) and / or inhibits expression of the marker (M), in that said linkage can be selectively cleaved by factors secreted by target cells so as to permit expression of the marker (M) or entry of the therapeutic agent (M) into said target cells, and in that the terminal group (W) provides for the stability of the compound (W-Z-M) in the serum and circulating blood.

This application provides methods of improving gene therapy by combining gene therapy with active site-specific chaperones (ASSCs). The ASSC increases the stability and efficiency of the protein encoded by the recombinant gene that is administered.

The invention discloses a preparation method of a cobalt electro-catalysis oxygen reduction material wrapping a nitrogen-doped carbon nanotube. The method includes the following steps of making melamine, cobalt nitrate hexahydrate and glucose react to obtain a self-assembled melamine precursor, and baking the self-assembled melamine precursor to obtain a cobalt nanoparticle hybrid material electro-catalysis oxygen reduction agent wrapping the nitrogen-doped carbon nanotube. In preparation of the precursor, a self-assembled body can be formed through the electrostatic interaction and the hydrogen-bond interaction by means of nitrate radicals and melamine under the acid condition, cobalt ions are adsorbed into the self-assembled body, ordered arrangement and assembly of materials in the high-temperature baking process are facilitated by the pre-assembled precursor, and therefore more catalysis active sites are exposed, and the improvement of the performance of a catalyst is facilitated.

New spin-on, bonding compositions and methods of using those compositions are provided. The cured bonding compositions comprise a crosslinked oxazoline (either crosslinked with another oxazoline or with a crosslinking agent), and can be used to bond an active wafer to a carrier wafer or substrate to assist in protecting the active wafer and its active sites during subsequent processing and handling. The compositions form bonding layers that are chemically and thermally resistant, but that can be thermally decomposed at 285° C. or higher to allow the wafers to slide apart at the appropriate stage in the fabrication process.