8 results about "Soft lithography" patented technology

The invention relates to the technical field of tissue engineering, and particularly discloses a lamina-free three-dimensional aneurysm chip as well as a preparation method and application thereof. The method comprises the following steps: firstly, designing a non-layered three-dimensional aneurysm chip mold which comprises a semi-aneurysm part capable of being accurately matched with aneurysm physiological structural characteristics, then preparing a non-layered three-dimensional aneurysm chip mold blank by adopting a photocuring 3D printing technology, and then coating a photosensitive resin mixture to obtain the non-layered three-dimensional aneurysm chip mold. Laminated lines on the surface layer of the mold blank are eliminated through the steps of spin coating treatment, ultraviolet curing and the like, the roughness of the surface of the mold blank is optimized, and finally the three-dimensional aneurysm chip is prepared through the soft photoetching technology. According to the method, through a series of combined processes of mold design, mold blank 3D printing, mold blank layer grain elimination and soft photoetching of the chip, the recarving precision of the three-dimensional aneurysm chip is remarkably improved, and the strict requirement of an aneurysm in-vitro simulation experiment for the structural precision is met; the technical defects that an existing three-dimensional aneurysm chip is low in precision, obvious in lamina and the like are overcome.

This invention relates to a microfluidic chip driven by acoustically excited droplet-shaped microbubbles and its fabrication process. A droplet-shaped microbubble array is obtained through microfluidic chip structure design. Under acoustic excitation, the microbubbles oscillate, generating asymmetric acoustic-flow vortices, which in turn directionally drive the microfluidic fluid within the microchannels. The flow rate can be linearly controlled by adjusting the driving voltage, offering advantages such as non-contact, pollution-free, and highly efficient directional driving. The fabrication process is based on MEMS technology, employing nitrogen plasma bonding and soft lithography, resulting in low cost and suitability for mass production. This invention solves the problem of the lack of directionality in traditional symmetrical acoustic-flow vortices and can be widely applied in fields such as biomedicine, drug delivery, and chemical analysis.

The invention relates to the technical field of biomedical detection, in particular to a micro-fluidic chip manufacturing method and an application method based on a 3D printing technology, and the method comprises the following steps: manufacturing a micro-fluidic chip through combination of 3D printing and soft lithography, optimizing a liquid drop generation and detection process, and developing a YOLO-Drop deep learning model to realize automatic identification and counting of liquid drop targets. According to the invention, the manufacturing cost and time of the micro-fluidic chip can be obviously reduced, the droplet generation precision and detection efficiency are improved, and meanwhile, ultra-sensitive protein detection is realized through intelligent analysis. Experimental verification shows that the detection limit of the system reaches 0.015 fM, the recovery rate range is 94.0%-116.96%, the coefficient of variation is lower than 10%, and an innovative solution is provided for biomedical detection.

The present invention relates to a method of manufacturing a single-layered ultra-thin film anti-reflection sticker by a soft etching process, and an ultra-thin film anti-reflection sticker. To this end, the present invention provides a method of manufacturing an ultra-thin film anti-reflection sticker, characterized by comprising: a step of manufacturing a master mold, the master mold being a mold structure in which a plurality of hemispherical shapes are formed on an upper surface; a sticker material coating step of coating a sticker forming material on the upper surface of the master mold; a cured film covering step of covering a cured film on an upper surface of the sticker forming material; a curing step of curing the sticker forming material; a cured film removing step of removing the cured film from the sticker forming material; and a sticker peeling step of peeling the cured sticker forming material from the master mold, and the peeled sticker forming material is formed as a single-layered ultra-thin film anti-reflection sticker in which a plurality of anti-reflection structures are formed on an upper surface.

The invention discloses an optical fiber end face microstructure precise packaging method based on a double-layer PDMS mold. The method comprises the following steps that 1, a micro-groove mold is prepared on a silicon wafer through the photoetching technology, a PDMS bottom layer with a micro-groove is obtained through transfer printing, a micro-cylinder mold is prepared on the silicon wafer through the photoetching technology, and a PDMS top layer with a through hole is obtained through transfer printing; step 2, concentrically aligning and bonding the PDMS top layer with the through hole and the PDMS bottom layer with the micro groove under a microscope to form a composite die with a guide hole-micro cavity structure; and step 3, filling the cavity with ultraviolet curing resin, vertically inserting the optical fiber into the top guide hole and abutting against the bottom groove, and demolding after ultraviolet curing to realize precise integration of the microstructure on the end face of the optical fiber. According to the method, a double-layer PDMS composite mold is prepared by using a soft lithography technology, and blind-mating self-alignment packaging of the optical fiber is realized through physical limiting.

A method of manufacturing a monolayer-structured ultra-thin antireflection sticker through a soft lithography process and an ultra-thin antireflection sticker, includes: a master mold manufacturing step in which a master mold having a plurality of semi-spherical mold structures on an upper surface thereof is manufactured; a sticker material coating step in which a sticker material is coated onto the upper surface of the master mold; a cured film covering step in which a cured film is disposed to cover an upper surface of the sticker material; a curing step in which the sticker material is cured; a cured film removal step in which the cured film is removed from the sticker material; and a sticker peeling step in which the cured sticker material is peeled off the master mold, the peeled-off sticker material forming a monolayer-structured ultra-thin antireflection sticker having a plurality of antireflective structures on an upper surface thereof.

The application provides a preparation method of a biomimetic microfluidic device for studying fluid migration in carbonate rock micro-nano cracks, comprising the following steps: step one, analyzing and classifying scanning electron microscope images of carbonate rock core slices by a random forest algorithm to generate a chip design drawing; step two, based on the chip design drawing, adopting soft lithography technology, copying crack structures through a photoresist template and polydimethylsiloxane, and bonding with a glass substrate to form a microfluidic chip; step three, injecting a precursor liquid containing calcite nanocrystal seeds into the flow channel of the microfluidic chip, fixing the crystal seeds by ultraviolet irradiation, and realizing calcium carbonate mineral precipitation by injecting a saturated equilibrium liquid to form a biomimetic microfluidic device. The application can be used for coupling research of all fluids and carbonate rock cracks, and provides a method for restoring the structure of carbonate rock cracks to the greatest extent and a method for cloning the properties of rock surfaces.

The present invention relates to an organ-on-a-chip device for mimicking the bone marrow microenvironment (1) comprising: a perivascular region (2) representing the perivascular niche; a central region (3) representing the central niche; an endosteal region (4) representing the endosteal niche; at least one fluid inlet channel (5); at least one channel for injecting cells, fluids and hydrogels (6) for each region (2, 3, 4); at least one containment port (7) to allow independent filling of the at least one channel for injecting cells, fluids and hydrogels (6); and at least one fluid outlet channel (8). The present invention also relates to a method for producing the device moulds using additive manufacturing, a method for producing the device from PDMS using soft lithography, a method for filling the device with hydrogel and performing three-dimensional culture, and use of the device.